Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0010997

Physical Location & Seq

Genomic Coordinates Reverse (-)

598210 .. 598782

Gene Structure 573 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1005400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 573 bp

ATGTTACCTATAAAAACGTTTCTCGCACACTTTCGCAACTACCAACTAACTCAACAAACCCCATCTCTCGACATGACTCGCCCTTTGAGATTGTTAAGCGCCTTCAAGCCTTCCTCGCTGTTTTCCGGACCGGAAGATGAACCCTCGTCCTCCGCCACCATGGGCTCAGACTCCGCTTTAGTCCTCCTCGCCCTCTTCTGCGCTCTCGTCTGCGTCCTCGGCCTCGTCGTCATCGCTCGTTGCGTCTGGCTTCGCCGCCTCACCGCTGCCGCCACAACTACCACACCTCCTCTTCCTCCGCCACCGCCGCCAAATAAGGGCTTGAAGAAAAAGGTCCTACGCTCTCTCCCGAAGCTTACGTACACGGCGGACTGCACTTCCAAGTTCACGGAGTGCGCCATCTGCCTCACCGAGTTCGCTTCCGGGGACGAGATCCGGGAGCTTCCGCAGTGTGGCCACGGCTTCCACGTTGCGTGCATCGACACGTGGCTCCGGTCGCACTCGTCCTGCCCGTCCTGCCGTCAGGTTCCGGAGATCTCTAATAAGTGCCACAAGTGTGGGGGTTACTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009266 GO:0009409 GO:0009628 GO:0009909 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010498 GO:0010966 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0032879 GO:0033554 GO:0036211 GO:0042221 GO:0042493 GO:0043161 GO:0043170 GO:0043269 GO:0043412 GO:0043632 GO:0044070 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044464 GO:0048580 GO:0048831 GO:0050789 GO:0050793 GO:0050896 GO:0051049 GO:0051239 GO:0051603 GO:0051716 GO:0061630 GO:0061659 GO:0065007 GO:0070417 GO:0070647 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901700 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

191

Amino Acids

20.72

Weight (kDa)

8.87

Isoelectric Point (pI)

64.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-rbx1	PF12678	125 - 174	3.8e-08	RING-H2 zinc finger domain
zf-RING_2	PF13639	131 - 174	1.6e-15	Ring finger domain
zf-RING_11	PF17123	131 - 160	1.8e-11	RING-like zinc finger
zf-C3HC4_2	PF13923	131 - 173	1.9e-09	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	132 - 173	1.5e-07	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010997)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20823 AT1G76410
fragaria_vesca	FvH4_1g10140
malus_domestica	MD02G1113400.v1.1 MD06G1005400.v1.1 MD15G1228600.v1.1
prunus_persica	Prupe.5G006700_v2.0.a1 Prupe.7G185500_v2.0.a1
pyrus_communis	pycom02g08840
rosa_chinensis	RchiOBHm_Chr2g0096911
rosa_laevigata	RLG00000016682
rosa_multiflora	Rmu_sc0033632.1_g000003 Rmu_ssc0000215.1_g000003
rosa_roxburghii	Rroxscaffold_2G00145160
rosa_rugosa	Rorug02G0061800
rosa_samantha	Rh2AG109800 Rh2BG111700 Rh2CG113800 Rh2DG112900
rosa_wichuraiana	Rw2G008480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccIII	TCCGGA	2 cut(s)	125, 529
AclI	AACGTT	1 cut(s)	17
AclWI	GGATC	1 cut(s)	427
AcoI	YGGCCR	1 cut(s)	454
AcvI	CACGTG	1 cut(s)	486
AdeI	CACNNNGTG	1 cut(s)	393
AfaI	GTAC	1 cut(s)	362
AfiI	CCNNNNNNNGG	2 cut(s)	317, 452
AflIII	ACRYGT	1 cut(s)	483
AgsI	TTSAA	2 cut(s)	106, 325
AleI	CACNNNNGTG	1 cut(s)	555
AluBI	AGCT	2 cut(s)	355, 442
AluI	AGCT	2 cut(s)	355, 442
AlwI	GGATC	1 cut(s)	427
Aor13HI	TCCGGA	2 cut(s)	125, 529
AoxI	GGCC	2 cut(s)	220, 454
ApeKI	GCWGC	1 cut(s)	266
ArsI	GACNNNNNNTTYG	2 cut(s)	67, 99
AspLEI	GCGC	3 cut(s)	101, 203, 398
AspS9I	GGNCC	2 cut(s)	128, 334
AsuC2I	CCSGG	2 cut(s)	424, 437
AsuHPI	GGTGA	2 cut(s)	253, 400
AvaII	GGWCC	2 cut(s)	128, 334
BalI	TGGCCA	1 cut(s)	456
BanII	GRGCYC	1 cut(s)	167
BarI	GAAGNNNNNNTAC	2 cut(s)	344, 376
BbrPI	CACGTG	1 cut(s)	486
BbvI	GCAGC	1 cut(s)	253
BccI	CCATC	2 cut(s)	70, 407
BceAI	ACGGC	3 cut(s)	381, 475, 504
BcnI	CCSGG	2 cut(s)	424, 437
BfoI	RGCGCY	1 cut(s)	102
BglII	AGATCT	1 cut(s)	534
BisI	GCNGC	4 cut(s)	256, 267, 270, 308
BlsI	GCNGC	4 cut(s)	257, 268, 271, 309
Bme1390I	CCNGG	2 cut(s)	424, 437
Bme18I	GGWCC	2 cut(s)	128, 334
BmgT120I	GGNCC	2 cut(s)	128, 334
BmiI	GGNNCC	2 cut(s)	491, 528
BmrFI	CCNGG	2 cut(s)	424, 437
BmsI	GCATC	1 cut(s)	486
BpuMI	CCSGG	2 cut(s)	424, 437
BsaAI	YACGTR	2 cut(s)	360, 486
BsaJI	CCNNGG	4 cut(s)	159, 217, 423, 457
BsaWI	WCCGGW	4 cut(s)	125, 130, 492, 529
BsaXI	ACNNNNNCTCC	4 cut(s)	271, 301, 330, 360
Bsc4I	CCNNNNNNNGG	2 cut(s)	317, 452
BseAI	TCCGGA	2 cut(s)	125, 529
BseDI	CCNNGG	4 cut(s)	159, 217, 423, 457
BseLI	CCNNNNNNNGG	2 cut(s)	317, 452
BseMII	CTCAG	1 cut(s)	180
BseRI	GAGGAG	2 cut(s)	176, 279
BseXI	GCAGC	1 cut(s)	253
BsgI	GTGCAG	1 cut(s)	358
Bsh1285I	CGRYCG	1 cut(s)	497
BshFI	GGCC	2 cut(s)	222, 456
BsiEI	CGRYCG	1 cut(s)	497
BsiSI	CCGG	6 cut(s)	126, 131, 423, 436, 493, 530
BslFI	GGGAC	1 cut(s)	440
BslI	CCNNNNNNNGG	2 cut(s)	317, 452
BsmFI	GGGAC	1 cut(s)	440
BsnI	GGCC	2 cut(s)	222, 456
Bsp1286I	GDGCHC	1 cut(s)	167
Bsp13I	TCCGGA	2 cut(s)	125, 529
Bsp143I	GATC	2 cut(s)	432, 534
Bsp19I	CCATGG	1 cut(s)	159
BspANI	GGCC	2 cut(s)	222, 456
BspCNI	CTCAG	1 cut(s)	179
BspEI	TCCGGA	2 cut(s)	125, 529
BspLI	GGNNCC	2 cut(s)	491, 528
BspPI	GGATC	1 cut(s)	427
BssECI	CCNNGG	4 cut(s)	159, 217, 423, 457
BssMI	GATC	2 cut(s)	432, 534
BssT1I	CCWWGG	1 cut(s)	159
Bst6I	CTCTTC	2 cut(s)	200, 297
BstBAI	YACGTR	2 cut(s)	360, 486
BstC8I	GCNNGC	1 cut(s)	475
BstDEI	CTNAG	1 cut(s)	166
BstDSI	CCRYGG	2 cut(s)	159, 457
BstH2I	RGCGCY	1 cut(s)	102
BstHHI	GCGC	3 cut(s)	101, 203, 398
BstKTI	GATC	2 cut(s)	435, 537
BstMBI	GATC	2 cut(s)	432, 534
BstMCI	CGRYCG	1 cut(s)	497
BstMWI	GCNNNNNNNGC	6 cut(s)	115, 219, 307, 402, 496, 516
BstSCI	CCNGG	2 cut(s)	422, 435
BstSNI	TACGTA	1 cut(s)	360
BstV1I	GCAGC	1 cut(s)	253
BstX2I	RGATCY	2 cut(s)	432, 534
BstXI	CCANNNNNNTGG	1 cut(s)	557
BstYI	RGATCY	2 cut(s)	432, 534
BsuRI	GGCC	2 cut(s)	222, 456
BtgI	CCRYGG	2 cut(s)	159, 457
BtgZI	GCGATG	1 cut(s)	217
BtsI	GCAGTG	1 cut(s)	455
BtsIMutI	CAGTG	1 cut(s)	455
Cac8I	GCNNGC	1 cut(s)	475
CfoI	GCGC	3 cut(s)	101, 203, 398
Cfr13I	GGNCC	2 cut(s)	128, 334
CpoI	CGGWCCG	1 cut(s)	128
CseI	GACGC	2 cut(s)	202, 232
Csp6I	GTAC	1 cut(s)	361
CspI	CGGWCCG	1 cut(s)	128
CviAII	CATG	2 cut(s)	73, 160
CviQI	GTAC	1 cut(s)	361
DdeI	CTNAG	1 cut(s)	166
DpnI	GATC	2 cut(s)	434, 536
DpnII	GATC	2 cut(s)	432, 534
DraIII	CACNNNGTG	1 cut(s)	393
EaeI	YGGCCR	1 cut(s)	454
Eam1104I	CTCTTC	2 cut(s)	200, 297
EarI	CTCTTC	2 cut(s)	200, 297
EciI	GGCGGA	3 cut(s)	142, 288, 383
Eco105I	TACGTA	1 cut(s)	360
Eco130I	CCWWGG	1 cut(s)	159
Eco24I	GRGCYC	1 cut(s)	167
Eco47I	GGWCC	2 cut(s)	128, 334
Eco72I	CACGTG	1 cut(s)	486
EcoO109I	RGGNCCY	1 cut(s)	334
EcoT14I	CCWWGG	1 cut(s)	159
EcoT38I	GRGCYC	1 cut(s)	167
ErhI	CCWWGG	1 cut(s)	159
FaeI	CATG	2 cut(s)	76, 163
FaiI	YATR	3 cut(s)	11, 74, 161
FaqI	GGGAC	1 cut(s)	440
FatI	CATG	2 cut(s)	72, 159
Fnu4HI	GCNGC	4 cut(s)	256, 267, 270, 308
FriOI	GRGCYC	1 cut(s)	167
Fsp4HI	GCNGC	4 cut(s)	256, 267, 270, 308
GlaI	GCGC	3 cut(s)	100, 202, 397
GluI	GCNGC	4 cut(s)	256, 267, 270, 308
HaeII	RGCGCY	1 cut(s)	102
HaeIII	GGCC	2 cut(s)	222, 456
HapII	CCGG	6 cut(s)	126, 131, 423, 436, 493, 530
HgaI	GACGC	2 cut(s)	202, 232
HhaI	GCGC	3 cut(s)	101, 203, 398
Hin1II	CATG	2 cut(s)	76, 163
Hin6I	GCGC	3 cut(s)	99, 201, 396
HinP1I	GCGC	3 cut(s)	99, 201, 396
HindIII	AAGCTT	1 cut(s)	353
HinfI	GANTC	2 cut(s)	76, 170
HpaII	CCGG	6 cut(s)	126, 131, 423, 436, 493, 530
HphI	GGTGA	2 cut(s)	253, 400
Hpy166II	GTNNAC	2 cut(s)	363, 387
Hpy188I	TCNGA	1 cut(s)	169
Hpy188III	TCNNGA	4 cut(s)	68, 126, 349, 530
Hpy8I	GTNNAC	2 cut(s)	363, 387
Hpy99I	CGWCG	1 cut(s)	230
HpyAV	CCTTC	2 cut(s)	112, 120
HpyCH4IV	ACGT	4 cut(s)	17, 359, 468, 485
HpyCH4V	TGCA	2 cut(s)	375, 477
HpyF10VI	GCNNNNNNNGC	6 cut(s)	115, 219, 307, 402, 496, 516
HpyF3I	CTNAG	1 cut(s)	166
HpySE526I	ACGT	4 cut(s)	17, 359, 468, 485
Hsp92II	CATG	2 cut(s)	76, 163
HspAI	GCGC	3 cut(s)	99, 201, 396
Kpn2I	TCCGGA	2 cut(s)	125, 529
Kzo9I	GATC	2 cut(s)	432, 534
LmnI	GCTCC	2 cut(s)	439, 495
Lsp1109I	GCAGC	1 cut(s)	253
LweI	GCATC	1 cut(s)	486
MaeII	ACGT	4 cut(s)	17, 359, 468, 485
MaeIII	GTNAC	2 cut(s)	3, 563
MalI	GATC	2 cut(s)	434, 536
MboI	GATC	2 cut(s)	432, 534
MboII	GAAGA	4 cut(s)	146, 187, 284, 337
MflI	RGATCY	2 cut(s)	432, 534
MhlI	GDGCHC	1 cut(s)	167
MlsI	TGGCCA	1 cut(s)	456
MluNI	TGGCCA	1 cut(s)	456
MlyI	GAGTC	2 cut(s)	70, 164
Mox20I	TGGCCA	1 cut(s)	456
MroI	TCCGGA	2 cut(s)	125, 529
MscI	TGGCCA	1 cut(s)	456
MseI	TTAA	2 cut(s)	95, 571
MslI	CAYNNNNRTG	1 cut(s)	555
Msp20I	TGGCCA	1 cut(s)	456
MspA1I	CMGCKG	1 cut(s)	266
MspI	CCGG	6 cut(s)	126, 131, 423, 436, 493, 530
MspR9I	CCNGG	2 cut(s)	424, 437
MwoI	GCNNNNNNNGC	6 cut(s)	115, 219, 307, 402, 496, 516
NciI	CCSGG	2 cut(s)	424, 437
NcoI	CCATGG	1 cut(s)	159
NdeII	GATC	2 cut(s)	432, 534
NlaIII	CATG	2 cut(s)	76, 163
NlaIV	GGNNCC	2 cut(s)	491, 528
NmeAIII	GCCGAG	1 cut(s)	198
OliI	CACNNNNGTG	1 cut(s)	555
PcsI	WCGNNNNNNNCGW	4 cut(s)	225, 240, 465, 509
PfoI	TCCNGGA	1 cut(s)	435
PkrI	GCNGC	4 cut(s)	257, 268, 271, 309
PleI	GAGTC	2 cut(s)	70, 164
PmaCI	CACGTG	1 cut(s)	486
PmlI	CACGTG	1 cut(s)	486
PpsI	GAGTC	2 cut(s)	70, 164
Ppu21I	YACGTR	2 cut(s)	360, 486
PpuMI	RGGWCCY	1 cut(s)	334
Psp1406I	AACGTT	1 cut(s)	17
Psp5II	RGGWCCY	1 cut(s)	334
PspCI	CACGTG	1 cut(s)	486
PspN4I	GGNNCC	2 cut(s)	491, 528
PspPI	GGNCC	2 cut(s)	128, 334
PspPPI	RGGWCCY	1 cut(s)	334
PsuI	RGATCY	2 cut(s)	432, 534
RsaI	GTAC	1 cut(s)	362
RsaNI	GTAC	1 cut(s)	361
RseI	CAYNNNNRTG	1 cut(s)	555
Rsr2I	CGGWCCG	1 cut(s)	128
RsrII	CGGWCCG	1 cut(s)	128
SaqAI	TTAA	2 cut(s)	95, 571
SatI	GCNGC	4 cut(s)	256, 267, 270, 308
Sau3AI	GATC	2 cut(s)	432, 534
Sau96I	GGNCC	2 cut(s)	128, 334
SchI	GAGTC	2 cut(s)	70, 164
ScrFI	CCNGG	2 cut(s)	424, 437
SduI	GDGCHC	1 cut(s)	167
SfaNI	GCATC	1 cut(s)	486
SinI	GGWCC	2 cut(s)	128, 334
SmiMI	CAYNNNNRTG	1 cut(s)	555
SnaBI	TACGTA	1 cut(s)	360
StyD4I	CCNGG	2 cut(s)	422, 435
StyI	CCWWGG	1 cut(s)	159
TaiI	ACGT	4 cut(s)	20, 362, 471, 488
TaqI	TCGA	2 cut(s)	69, 480
TauI	GCSGC	3 cut(s)	258, 272, 310
Tru1I	TTAA	2 cut(s)	95, 571
Tru9I	TTAA	2 cut(s)	95, 571
TscAI	CASTG	1 cut(s)	455
TseI	GCWGC	1 cut(s)	266
TspDTI	ATGAA	1 cut(s)	153
TspGWI	ACGGA	1 cut(s)	404
TspRI	CASTG	1 cut(s)	455
VpaK11BI	GGWCC	2 cut(s)	128, 334

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.