MD15G1228600.v1.1

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010997
Physical Location & Seq
Reverse (-)
18638066 .. 18638716
651 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1228600.v1.1.491

Sequence Viewer

Length: 651 bp
ATGCCTCGCTCTCAAAGATTTCTAGGCAGCAGCACCCCCTCCGCCAACTTCTCAAACATAACAGCAGCTCCGCCTCCTGAGGCCGTGGTTCTCGAGTCGGACTTCGTCGTCATACTGGCAGCGCTGCTCTGCGCCGTCATATGCGTGGTGGGTCTACTCGCCGTCGCACGCTGCGCCTGGCTCCGCCGCGGATCCGAGGGCAGAGCCGCTATCGGATCGGCTTCTTCATCCGCCAACAAGGGCCTCAAGAAGAAGGTCCTCCAGTCCCTTCCGAAGTTCACGTACGGAACCGGCGGCGCCGAGCCCCCGCCGAAGCTGGGTTCGGAGTGCGCCATCTGCTTAGGGGAGTTCGCGGAGGGGCACGAGATCAGGGTTCTGCCGCAGTGCGGGCACGTGTTCCATGTCGGGTGCATTGACACGTGGCTTGGATCCCACTCCTCGTGCCCGTCGTGCCGGCAGATCTTGGTGGTGGCTCGGTGTCACAAGTGCGGTGGGTTTCCGGCTCCAATATCAGAGGCGGAGCTCAAGGCTCACCAGGACCACACCAACCATTCAGCTCCTCCTCCGGCAGCGGCGGCTGCAGCTGCACCCAGTACTGCCATTAGTATCAGTTCTTCTAATACGGCTACAACTACCAATTTTCTGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

217

Amino Acids

22.28

Weight (kDa)

8.52

Isoelectric Point (pI)

57.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 109 - 152 3.9e-15 Ring finger domain
zf-RING_11 PF17123 109 - 138 2.1e-11 RING-like zinc finger
zf-C3HC4_2 PF13923 109 - 151 4.4e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 109 - 153 3.7e-06 zinc-RING finger domain
zf-RING_UBOX PF13445 110 - 149 1.6e-06 RING-type zinc-finger
zf-C3HC4 PF00097 110 - 151 1.8e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 123 - 152 8.5e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 107
AccB1I GGYRCC 1 cut(s) 296
AccI GTMKAC 1 cut(s) 154
AccII CGCG 2 cut(s) 189, 353
AclWI GGATC 5 cut(s) 186, 199, 223, 423, 436
AcvI CACGTG 2 cut(s) 394, 420
AcyI GRCGYC 1 cut(s) 297
AfaI GTAC 2 cut(s) 284, 595
AfeI AGCGCT 1 cut(s) 123
AfiI CCNNNNNNNGG 2 cut(s) 317, 386
AflIII ACRYGT 2 cut(s) 393, 417
AjnI CCWGG 2 cut(s) 176, 534
AluBI AGCT 5 cut(s) 68, 316, 523, 557, 584
AluI AGCT 5 cut(s) 68, 316, 523, 557, 584
Alw21I GWGCWC 1 cut(s) 525
AlwI GGATC 5 cut(s) 186, 199, 223, 423, 436
Ama87I CYCGRG 1 cut(s) 92
Aor51HI AGCGCT 1 cut(s) 123
AoxI GGCC 2 cut(s) 81, 241
AspLEI GCGC 5 cut(s) 124, 134, 176, 299, 332
AspS9I GGNCC 3 cut(s) 241, 256, 538
AsuHPI GGTGA 1 cut(s) 524
AvaI CYCGRG 1 cut(s) 92
AvaII GGWCC 2 cut(s) 256, 538
AxyI CCTNAGG 1 cut(s) 78
BaeGI GKGCMC 3 cut(s) 363, 393, 446
BamHI GGATCC 2 cut(s) 191, 428
BanI GGYRCC 1 cut(s) 296
BanII GRGCYC 2 cut(s) 306, 525
BarI GAAGNNNNNNTAC 4 cut(s) 266, 298, 598, 630
BauI CACGAG 2 cut(s) 362, 439
BbrPI CACGTG 2 cut(s) 394, 420
Bbv12I GWGCWC 1 cut(s) 525
BccI CCATC 1 cut(s) 341
BceAI ACGGC 4 cut(s) 68, 119, 146, 639
BciT130I CCWGG 2 cut(s) 178, 536
BfaI CTAG 1 cut(s) 23
BfmI CTRYAG 1 cut(s) 579
BfoI RGCGCY 2 cut(s) 125, 300
BglII AGATCT 1 cut(s) 459
BmcAI AGTACT 1 cut(s) 595
Bme1390I CCNGG 2 cut(s) 178, 536
Bme18I GGWCC 2 cut(s) 256, 538
BmeT110I CYCGRG 1 cut(s) 92
BmgT120I GGNCC 3 cut(s) 241, 256, 538
BmiI GGNNCC 6 cut(s) 182, 193, 289, 298, 430, 504
BmrFI CCNGG 2 cut(s) 178, 536
BmrI ACTGGG 1 cut(s) 585
BmuI ACTGGG 1 cut(s) 585
BpmI CTGGAG 1 cut(s) 245
Bpu10I CCTNAGC 1 cut(s) 340
BpuEI CTTGAG 2 cut(s) 230, 509
BsaAI YACGTR 3 cut(s) 282, 394, 420
BsaHI GRCGYC 1 cut(s) 297
BsaJI CCNNGG 3 cut(s) 84, 187, 195
BsaXI ACNNNNNCTCC 2 cut(s) 52, 82
Bsc4I CCNNNNNNNGG 2 cut(s) 317, 386
Bse118I RCCGGY 2 cut(s) 290, 453
Bse1I ACTGG 3 cut(s) 120, 262, 591
Bse21I CCTNAGG 1 cut(s) 78
BseBI CCWGG 2 cut(s) 178, 536
BseDI CCNNGG 3 cut(s) 84, 187, 195
BseGI GGATG 1 cut(s) 227
BseLI CCNNNNNNNGG 2 cut(s) 317, 386
BseMII CTCAG 1 cut(s) 69
BseNI ACTGG 3 cut(s) 120, 262, 591
BseRI GAGGAG 3 cut(s) 427, 549, 552
BseSI GKGCMC 3 cut(s) 363, 393, 446
BseYI CCCAGC 1 cut(s) 316
BsgI GTGCAG 1 cut(s) 570
Bsh1236I CGCG 2 cut(s) 189, 353
BshFI GGCC 2 cut(s) 83, 243
BshNI GGYRCC 1 cut(s) 296
BsiHKAI GWGCWC 1 cut(s) 525
BsiHKCI CYCGRG 1 cut(s) 92
BsiSI CCGG 4 cut(s) 291, 454, 500, 566
BsiWI CGTACG 1 cut(s) 282
BslFI GGGAC 1 cut(s) 250
BslI CCNNNNNNNGG 2 cut(s) 317, 386
BsmFI GGGAC 1 cut(s) 250
BsnI GGCC 2 cut(s) 83, 243
BsoBI CYCGRG 1 cut(s) 92
Bsp1286I GDGCHC 5 cut(s) 306, 363, 393, 446, 525
Bsp143I GATC 5 cut(s) 191, 215, 366, 428, 459
BspANI GGCC 2 cut(s) 83, 243
BspCNI CTCAG 1 cut(s) 70
BspFNI CGCG 2 cut(s) 189, 353
BspLI GGNNCC 6 cut(s) 182, 193, 289, 298, 430, 504
BspMAI CTGCAG 1 cut(s) 583
BspPI GGATC 5 cut(s) 186, 199, 223, 423, 436
BspT107I GGYRCC 1 cut(s) 296
BsrFI RCCGGY 2 cut(s) 290, 453
BsrI ACTGG 3 cut(s) 120, 262, 591
BssAI RCCGGY 2 cut(s) 290, 453
BssECI CCNNGG 3 cut(s) 84, 187, 195
BssMI GATC 5 cut(s) 191, 215, 366, 428, 459
BssNI GRCGYC 1 cut(s) 297
BssSI CACGAG 2 cut(s) 362, 439
Bst2BI CACGAG 2 cut(s) 362, 439
Bst2UI CCWGG 2 cut(s) 178, 536
BstACI GRCGYC 1 cut(s) 297
BstBAI YACGTR 3 cut(s) 282, 394, 420
BstC8I GCNNGC 3 cut(s) 169, 389, 455
BstDEI CTNAG 2 cut(s) 78, 340
BstDSI CCRYGG 2 cut(s) 84, 187
BstF5I GGATG 1 cut(s) 227
BstFNI CGCG 2 cut(s) 189, 353
BstH2I RGCGCY 2 cut(s) 125, 300
BstHHI GCGC 5 cut(s) 124, 134, 176, 299, 332
BstKTI GATC 5 cut(s) 194, 218, 369, 431, 462
BstMBI GATC 5 cut(s) 191, 215, 366, 428, 459
BstMWI GCNNNNNNNGC 8 cut(s) 173, 336, 388, 450, 575, 578, 581, 584
BstNI CCWGG 2 cut(s) 178, 536
BstSCI CCNGG 2 cut(s) 176, 534
BstSFI CTRYAG 1 cut(s) 579
BstSLI GKGCMC 3 cut(s) 363, 393, 446
BstUI CGCG 2 cut(s) 189, 353
BstX2I RGATCY 3 cut(s) 191, 428, 459
BstYI RGATCY 3 cut(s) 191, 428, 459
Bsu36I CCTNAGG 1 cut(s) 78
BsuRI GGCC 2 cut(s) 83, 243
BtgI CCRYGG 2 cut(s) 84, 187
BtsCI GGATG 1 cut(s) 227
BtsI GCAGTG 1 cut(s) 389
BtsIMutI CAGTG 1 cut(s) 389
Cac8I GCNNGC 3 cut(s) 169, 389, 455
CfoI GCGC 5 cut(s) 124, 134, 176, 299, 332
Cfr10I RCCGGY 2 cut(s) 290, 453
Cfr13I GGNCC 3 cut(s) 241, 256, 538
Cfr42I CCGCGG 1 cut(s) 190
Csp6I GTAC 2 cut(s) 283, 594
CspCI CAANNNNNGTGG 2 cut(s) 472, 507
CviAII CATG 1 cut(s) 401
CviQI GTAC 2 cut(s) 283, 594
DdeI CTNAG 2 cut(s) 78, 340
DinI GGCGCC 1 cut(s) 298
DpnI GATC 5 cut(s) 193, 217, 368, 430, 461
DpnII GATC 5 cut(s) 191, 215, 366, 428, 459
DrdI GACNNNNNNGTC 1 cut(s) 107
DseDI GACNNNNNNGTC 1 cut(s) 107
EciI GGCGGA 5 cut(s) 31, 60, 173, 220, 533
Ecl136II GAGCTC 1 cut(s) 523
Eco24I GRGCYC 2 cut(s) 306, 525
Eco47I GGWCC 2 cut(s) 256, 538
Eco47III AGCGCT 1 cut(s) 123
Eco53kI GAGCTC 1 cut(s) 523
Eco72I CACGTG 2 cut(s) 394, 420
Eco81I CCTNAGG 1 cut(s) 78
Eco88I CYCGRG 1 cut(s) 92
EcoICRI GAGCTC 1 cut(s) 523
EcoO109I RGGNCCY 2 cut(s) 241, 256
EcoRII CCWGG 2 cut(s) 176, 534
EcoT38I GRGCYC 2 cut(s) 306, 525
EgeI GGCGCC 1 cut(s) 298
EheI GGCGCC 1 cut(s) 298
FaeI CATG 1 cut(s) 404
FaiI YATR 5 cut(s) 59, 113, 140, 142, 402
FaqI GGGAC 1 cut(s) 250
FatI CATG 1 cut(s) 400
FauI CCCGC 2 cut(s) 315, 380
FauNDI CATATG 1 cut(s) 140
FblI GTMKAC 1 cut(s) 154
FokI GGATG 1 cut(s) 214
FriOI GRGCYC 2 cut(s) 306, 525
FspBI CTAG 1 cut(s) 23
GlaI GCGC 5 cut(s) 123, 133, 175, 298, 331
GsaI CCCAGC 1 cut(s) 320
GsuI CTGGAG 1 cut(s) 245
HaeII RGCGCY 2 cut(s) 125, 300
HaeIII GGCC 2 cut(s) 83, 243
HapII CCGG 4 cut(s) 291, 454, 500, 566
HhaI GCGC 5 cut(s) 124, 134, 176, 299, 332
Hin1I GRCGYC 1 cut(s) 297
Hin1II CATG 1 cut(s) 404
Hin6I GCGC 5 cut(s) 122, 132, 174, 297, 330
HinP1I GCGC 5 cut(s) 122, 132, 174, 297, 330
HinfI GANTC 1 cut(s) 95
HpaII CCGG 4 cut(s) 291, 454, 500, 566
HphI GGTGA 1 cut(s) 524
Hpy166II GTNNAC 2 cut(s) 155, 279
Hpy188I TCNGA 6 cut(s) 100, 196, 215, 273, 325, 514
Hpy188III TCNNGA 3 cut(s) 77, 92, 247
Hpy8I GTNNAC 2 cut(s) 155, 279
Hpy99I CGWCG 3 cut(s) 110, 167, 451
HpyAV CCTTC 2 cut(s) 247, 278
HpyCH4IV ACGT 3 cut(s) 281, 393, 419
HpyCH4V TGCA 3 cut(s) 411, 581, 587
HpyF10VI GCNNNNNNNGC 8 cut(s) 173, 336, 388, 450, 575, 578, 581, 584
HpyF3I CTNAG 2 cut(s) 78, 340
HpySE526I ACGT 3 cut(s) 281, 393, 419
Hsp92I GRCGYC 1 cut(s) 297
Hsp92II CATG 1 cut(s) 404
HspAI GCGC 5 cut(s) 122, 132, 174, 297, 330
KasI GGCGCC 1 cut(s) 296
KroI GCCGGC 1 cut(s) 453
KroNI GCCGGC 1 cut(s) 455
KspI CCGCGG 1 cut(s) 190
Kzo9I GATC 5 cut(s) 191, 215, 366, 428, 459
LmnI GCTCC 5 cut(s) 73, 186, 508, 520, 562
MaeI CTAG 1 cut(s) 23
MaeII ACGT 3 cut(s) 281, 393, 419
MaeIII GTNAC 1 cut(s) 479
MalI GATC 5 cut(s) 193, 217, 368, 430, 461
MboI GATC 5 cut(s) 191, 215, 366, 428, 459
MboII GAAGA 3 cut(s) 216, 262, 606
MflI RGATCY 3 cut(s) 191, 428, 459
MhlI GDGCHC 5 cut(s) 306, 363, 393, 446, 525
MluCI AATT 1 cut(s) 637
Mly113I GGCGCC 1 cut(s) 297
MlyI GAGTC 1 cut(s) 104
MmeI TCCRAC 1 cut(s) 78
MroNI GCCGGC 1 cut(s) 453
MseI TTAA 1 cut(s) 649
MslI CAYNNNNRTG 1 cut(s) 143
MspA1I CMGCKG 3 cut(s) 189, 572, 584
MspI CCGG 4 cut(s) 291, 454, 500, 566
MspR9I CCNGG 2 cut(s) 178, 536
MvaI CCWGG 2 cut(s) 178, 536
MvnI CGCG 2 cut(s) 189, 353
MwoI GCNNNNNNNGC 8 cut(s) 173, 336, 388, 450, 575, 578, 581, 584
NaeI GCCGGC 1 cut(s) 455
NarI GGCGCC 1 cut(s) 297
NdeI CATATG 1 cut(s) 140
NdeII GATC 5 cut(s) 191, 215, 366, 428, 459
NgoMIV GCCGGC 1 cut(s) 453
NlaIII CATG 1 cut(s) 404
NlaIV GGNNCC 6 cut(s) 182, 193, 289, 298, 430, 504
NmeAIII GCCGAG 1 cut(s) 325
NmuCI GTSAC 1 cut(s) 479
PaeR7I CTCGAG 1 cut(s) 92
PcsI WCGNNNNNNNCGW 1 cut(s) 446
PdiI GCCGGC 1 cut(s) 455
Pfl23II CGTACG 1 cut(s) 282
PflFI GACNNNGTC 1 cut(s) 104
PleI GAGTC 1 cut(s) 103
PluTI GGCGCC 1 cut(s) 300
PmaCI CACGTG 2 cut(s) 394, 420
PmlI CACGTG 2 cut(s) 394, 420
PpsI GAGTC 1 cut(s) 103
Ppu21I YACGTR 3 cut(s) 282, 394, 420
PpuMI RGGWCCY 1 cut(s) 256
Psp124BI GAGCTC 1 cut(s) 525
Psp5II RGGWCCY 1 cut(s) 256
Psp6I CCWGG 2 cut(s) 176, 534
PspCI CACGTG 2 cut(s) 394, 420
PspFI CCCAGC 1 cut(s) 316
PspGI CCWGG 2 cut(s) 176, 534
PspLI CGTACG 1 cut(s) 282
PspN4I GGNNCC 6 cut(s) 182, 193, 289, 298, 430, 504
PspPI GGNCC 3 cut(s) 241, 256, 538
PspPPI RGGWCCY 1 cut(s) 256
PstI CTGCAG 1 cut(s) 583
PsuI RGATCY 3 cut(s) 191, 428, 459
PsyI GACNNNGTC 1 cut(s) 104
PvuII CAGCTG 1 cut(s) 584
RsaI GTAC 2 cut(s) 284, 595
RsaNI GTAC 2 cut(s) 283, 594
RseI CAYNNNNRTG 1 cut(s) 143
SacI GAGCTC 1 cut(s) 525
SacII CCGCGG 1 cut(s) 190
SaqAI TTAA 1 cut(s) 649
Sau3AI GATC 5 cut(s) 191, 215, 366, 428, 459
Sau96I GGNCC 3 cut(s) 241, 256, 538
ScaI AGTACT 1 cut(s) 595
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 2 cut(s) 178, 536
SduI GDGCHC 5 cut(s) 306, 363, 393, 446, 525
SetI ASST 9 cut(s) 70, 258, 284, 318, 396, 422, 525, 559, 586
SfcI CTRYAG 1 cut(s) 579
SfoI GGCGCC 1 cut(s) 298
Sfr274I CTCGAG 1 cut(s) 92
Sfr303I CCGCGG 1 cut(s) 190
SgrBI CCGCGG 1 cut(s) 190
SinI GGWCC 2 cut(s) 256, 538
SlaI CTCGAG 1 cut(s) 92
SmiMI CAYNNNNRTG 1 cut(s) 143
SmlI CTYRAG 3 cut(s) 92, 245, 524
SmoI CTYRAG 3 cut(s) 92, 245, 524
Sse9I AATT 1 cut(s) 637
SspDI GGCGCC 1 cut(s) 296
SspMI CTAG 1 cut(s) 23
SstI GAGCTC 1 cut(s) 525
StyD4I CCNGG 2 cut(s) 176, 534
TaiI ACGT 3 cut(s) 284, 396, 422
TaqI TCGA 1 cut(s) 93
TasI AATT 1 cut(s) 637
TatI WGTACW 1 cut(s) 593
TauI GCSGC 6 cut(s) 189, 209, 297, 382, 575, 578
Tru1I TTAA 1 cut(s) 649
Tru9I TTAA 1 cut(s) 649
TscAI CASTG 1 cut(s) 389
TseFI GTSAC 1 cut(s) 479
Tsp45I GTSAC 1 cut(s) 479
TspDTI ATGAA 1 cut(s) 216
TspGWI ACGGA 1 cut(s) 300
TspRI CASTG 1 cut(s) 389
Tth111I GACNNNGTC 1 cut(s) 104
VpaK11BI GGWCC 2 cut(s) 256, 538
XhoI CTCGAG 1 cut(s) 92
XmiI GTMKAC 1 cut(s) 154
XspI CTAG 1 cut(s) 23
ZrmI AGTACT 1 cut(s) 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.