MD02G1113400.v1.1

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0010997
Physical Location & Seq
Reverse (-)
9206058 .. 9206705
648 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1113400.v1.1.491

Sequence Viewer

Length: 648 bp
ATGCCTCGCTCTCAGAGATTTCTAGGCAGCAGCACCCCCGCCACCAACTTCTCAACCGTATCGGCAGCTCCACCTCCTGAGGCCGTGGCTCTGGAGTCGGATTTAGTTGTCATACTAGCAGCGCTGCTCTGCGCCGTCATATGCGTGGTGGGTCTTCTCGCCGTCGCCCGCTGCGCCTGGCTCCGCCGCGGCCCCGGCGGCAGAGCCGCTATCGGCTCGGCTTCTTCATCCGCCAACAAGGGCCTCAAGAAGAAGGTCCTCCAGTCCCTCCCGAAGTTCACGTACGGATCCGGCGGCGCCGAGCCGCCGCCAAAGCTGGGTTCGGAGTGCGCCATCTGCTTAGGGGAGTTCGCGGAGGGGCACGAGATCAGGATTCTGCCGCAGTGCGGGCACGTGTTCCACGTTGGATGCATCGACACGTGGCTTGGGTCTCACTCCTCGTGCCCTTCGTGTCGGCAGATCTTGGTGGTTGCTCGGTGTCACAAGTGCGGCGGGTTTCCGGCTCCAATATCTGAATCTGAGGCGGAGCTCAAGGCTCGCCAGGACCACACCAACCATTCAGCTCCTCCTCCGGCGGCGGCTGCACCCACTACTGCCATTAGTATTAGTTCTTCTAATACGGCTCCAACTACAAATTTTCTCCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

216

Amino Acids

22.16

Weight (kDa)

8.69

Isoelectric Point (pI)

60.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 109 - 152 4.1e-15 Ring finger domain
zf-RING_11 PF17123 109 - 138 3e-11 RING-like zinc finger
zf-C3HC4_2 PF13923 109 - 151 4.8e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 109 - 153 4.6e-06 zinc-RING finger domain
zf-C3HC4 PF00097 110 - 151 1.4e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 110 - 149 2.9e-06 RING-type zinc-finger
zf-rbx1 PF12678 125 - 152 1.7e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 296
AccII CGCG 2 cut(s) 189, 353
AclWI GGATC 2 cut(s) 282, 295
AcsI RAATTY 1 cut(s) 634
AcvI CACGTG 2 cut(s) 394, 420
AcyI GRCGYC 1 cut(s) 297
AfaI GTAC 1 cut(s) 284
AfeI AGCGCT 1 cut(s) 123
AfiI CCNNNNNNNGG 2 cut(s) 317, 386
AflIII ACRYGT 2 cut(s) 393, 417
AjnI CCWGG 2 cut(s) 176, 540
AjuI GAANNNNNNNTTGG 2 cut(s) 304, 336
AluBI AGCT 4 cut(s) 68, 316, 529, 563
AluI AGCT 4 cut(s) 68, 316, 529, 563
Alw21I GWGCWC 1 cut(s) 531
Alw26I GTCTC 1 cut(s) 435
AlwI GGATC 2 cut(s) 282, 295
Aor51HI AGCGCT 1 cut(s) 123
AoxI GGCC 3 cut(s) 81, 190, 241
ApeKI GCWGC 7 cut(s) 27, 30, 65, 119, 124, 171, 581
ApoI RAATTY 1 cut(s) 634
AspLEI GCGC 5 cut(s) 124, 134, 176, 299, 332
AspS9I GGNCC 4 cut(s) 191, 241, 256, 544
AsuC2I CCSGG 1 cut(s) 195
AvaII GGWCC 2 cut(s) 256, 544
AxyI CCTNAGG 1 cut(s) 78
BaeGI GKGCMC 3 cut(s) 363, 393, 446
BamHI GGATCC 1 cut(s) 287
BanI GGYRCC 1 cut(s) 296
BanII GRGCYC 1 cut(s) 531
BarI GAAGNNNNNNTAC 4 cut(s) 266, 298, 595, 627
BauI CACGAG 2 cut(s) 362, 439
BbrPI CACGTG 2 cut(s) 394, 420
BbsI GAAGAC 1 cut(s) 146
Bbv12I GWGCWC 1 cut(s) 531
BbvI GCAGC 7 cut(s) 39, 42, 77, 111, 131, 158, 568
BccI CCATC 1 cut(s) 341
BceAI ACGGC 4 cut(s) 68, 119, 146, 636
BciT130I CCWGG 2 cut(s) 178, 542
BcnI CCSGG 1 cut(s) 195
BcoDI GTCTC 1 cut(s) 435
BfaI CTAG 2 cut(s) 23, 116
BfoI RGCGCY 2 cut(s) 125, 300
BglI GCCNNNNNGGC 1 cut(s) 198
BglII AGATCT 1 cut(s) 459
Bme1390I CCNGG 3 cut(s) 178, 195, 542
Bme18I GGWCC 2 cut(s) 256, 544
BmgT120I GGNCC 4 cut(s) 191, 241, 256, 544
BmiI GGNNCC 6 cut(s) 182, 193, 289, 298, 504, 624
BmrFI CCNGG 3 cut(s) 178, 195, 542
BmsI GCATC 2 cut(s) 398, 420
BpiI GAAGAC 1 cut(s) 146
BpmI CTGGAG 2 cut(s) 113, 245
Bpu10I CCTNAGC 1 cut(s) 340
BpuEI CTTGAG 2 cut(s) 230, 515
BpuMI CCSGG 1 cut(s) 195
BsaAI YACGTR 3 cut(s) 282, 394, 420
BsaHI GRCGYC 1 cut(s) 297
BsaI GGTCTC 1 cut(s) 435
BsaJI CCNNGG 3 cut(s) 84, 187, 193
Bsc4I CCNNNNNNNGG 2 cut(s) 317, 386
Bse1I ACTGG 1 cut(s) 262
Bse21I CCTNAGG 1 cut(s) 78
BseBI CCWGG 2 cut(s) 178, 542
BseDI CCNNGG 3 cut(s) 84, 187, 193
BseGI GGATG 2 cut(s) 227, 413
BseLI CCNNNNNNNGG 2 cut(s) 317, 386
BseMII CTCAG 3 cut(s) 26, 69, 510
BseNI ACTGG 1 cut(s) 262
BseRI GAGGAG 3 cut(s) 427, 555, 558
BseSI GKGCMC 3 cut(s) 363, 393, 446
BseXI GCAGC 7 cut(s) 39, 42, 77, 111, 131, 158, 568
BseYI CCCAGC 1 cut(s) 316
BsgI GTGCAG 1 cut(s) 567
Bsh1236I CGCG 2 cut(s) 189, 353
BshFI GGCC 3 cut(s) 83, 192, 243
BshNI GGYRCC 1 cut(s) 296
BsiHKAI GWGCWC 1 cut(s) 531
BsiSI CCGG 4 cut(s) 195, 291, 500, 572
BsiWI CGTACG 1 cut(s) 282
BslFI GGGAC 1 cut(s) 250
BslI CCNNNNNNNGG 2 cut(s) 317, 386
BsmAI GTCTC 1 cut(s) 435
BsmFI GGGAC 1 cut(s) 250
BsnI GGCC 3 cut(s) 83, 192, 243
Bso31I GGTCTC 1 cut(s) 435
Bsp1286I GDGCHC 4 cut(s) 363, 393, 446, 531
Bsp143I GATC 3 cut(s) 287, 366, 459
BspANI GGCC 3 cut(s) 83, 192, 243
BspCNI CTCAG 3 cut(s) 25, 70, 511
BspFNI CGCG 2 cut(s) 189, 353
BspLI GGNNCC 6 cut(s) 182, 193, 289, 298, 504, 624
BspPI GGATC 2 cut(s) 282, 295
BspT107I GGYRCC 1 cut(s) 296
BspTNI GGTCTC 1 cut(s) 435
BsrI ACTGG 1 cut(s) 262
BssECI CCNNGG 3 cut(s) 84, 187, 193
BssMI GATC 3 cut(s) 287, 366, 459
BssNI GRCGYC 1 cut(s) 297
BssSI CACGAG 2 cut(s) 362, 439
Bst2BI CACGAG 2 cut(s) 362, 439
Bst2UI CCWGG 2 cut(s) 178, 542
Bst4CI ACNGT 1 cut(s) 58
BstACI GRCGYC 1 cut(s) 297
BstBAI YACGTR 3 cut(s) 282, 394, 420
BstC8I GCNNGC 3 cut(s) 169, 389, 538
BstDEI CTNAG 4 cut(s) 12, 78, 340, 519
BstDSI CCRYGG 2 cut(s) 84, 187
BstF5I GGATG 2 cut(s) 227, 413
BstFNI CGCG 2 cut(s) 189, 353
BstH2I RGCGCY 2 cut(s) 125, 300
BstHHI GCGC 5 cut(s) 124, 134, 176, 299, 332
BstKTI GATC 3 cut(s) 290, 369, 462
BstMAI GTCTC 1 cut(s) 435
BstMBI GATC 3 cut(s) 287, 366, 459
BstMWI GCNNNNNNNGC 7 cut(s) 173, 195, 198, 313, 336, 388, 581
BstNI CCWGG 2 cut(s) 178, 542
BstSCI CCNGG 3 cut(s) 176, 193, 540
BstSLI GKGCMC 3 cut(s) 363, 393, 446
BstUI CGCG 2 cut(s) 189, 353
BstV1I GCAGC 7 cut(s) 39, 42, 77, 111, 131, 158, 568
BstV2I GAAGAC 1 cut(s) 146
BstX2I RGATCY 2 cut(s) 287, 459
BstYI RGATCY 2 cut(s) 287, 459
Bsu36I CCTNAGG 1 cut(s) 78
BsuRI GGCC 3 cut(s) 83, 192, 243
BtgI CCRYGG 2 cut(s) 84, 187
BtsCI GGATG 2 cut(s) 227, 413
BtsI GCAGTG 1 cut(s) 389
BtsIMutI CAGTG 1 cut(s) 389
Cac8I GCNNGC 3 cut(s) 169, 389, 538
CfoI GCGC 5 cut(s) 124, 134, 176, 299, 332
Cfr13I GGNCC 4 cut(s) 191, 241, 256, 544
Cfr42I CCGCGG 1 cut(s) 190
Csp6I GTAC 1 cut(s) 283
CviQI GTAC 1 cut(s) 283
DdeI CTNAG 4 cut(s) 12, 78, 340, 519
DinI GGCGCC 1 cut(s) 298
DpnI GATC 3 cut(s) 289, 368, 461
DpnII GATC 3 cut(s) 287, 366, 459
EciI GGCGGA 3 cut(s) 173, 220, 539
Ecl136II GAGCTC 1 cut(s) 529
Eco24I GRGCYC 1 cut(s) 531
Eco31I GGTCTC 1 cut(s) 435
Eco47I GGWCC 2 cut(s) 256, 544
Eco47III AGCGCT 1 cut(s) 123
Eco53kI GAGCTC 1 cut(s) 529
Eco72I CACGTG 2 cut(s) 394, 420
Eco81I CCTNAGG 1 cut(s) 78
EcoICRI GAGCTC 1 cut(s) 529
EcoO109I RGGNCCY 2 cut(s) 241, 256
EcoRII CCWGG 2 cut(s) 176, 540
EcoT22I ATGCAT 1 cut(s) 413
EcoT38I GRGCYC 1 cut(s) 531
EgeI GGCGCC 1 cut(s) 298
EheI GGCGCC 1 cut(s) 298
FaiI YATR 3 cut(s) 113, 140, 142
FaqI GGGAC 1 cut(s) 250
FauI CCCGC 4 cut(s) 46, 176, 380, 485
FauNDI CATATG 1 cut(s) 140
FokI GGATG 2 cut(s) 214, 420
FriOI GRGCYC 1 cut(s) 531
FspBI CTAG 2 cut(s) 23, 116
GlaI GCGC 5 cut(s) 123, 133, 175, 298, 331
GsaI CCCAGC 1 cut(s) 320
GsuI CTGGAG 2 cut(s) 113, 245
HaeII RGCGCY 2 cut(s) 125, 300
HaeIII GGCC 3 cut(s) 83, 192, 243
HapII CCGG 4 cut(s) 195, 291, 500, 572
HhaI GCGC 5 cut(s) 124, 134, 176, 299, 332
Hin1I GRCGYC 1 cut(s) 297
Hin6I GCGC 5 cut(s) 122, 132, 174, 297, 330
HinP1I GCGC 5 cut(s) 122, 132, 174, 297, 330
HinfI GANTC 3 cut(s) 95, 373, 515
HpaII CCGG 4 cut(s) 195, 291, 500, 572
Hpy166II GTNNAC 1 cut(s) 279
Hpy188I TCNGA 5 cut(s) 15, 100, 325, 514, 520
Hpy188III TCNNGA 5 cut(s) 77, 92, 247, 271, 370
Hpy8I GTNNAC 1 cut(s) 279
Hpy99I CGWCG 1 cut(s) 167
HpyAV CCTTC 2 cut(s) 247, 456
HpyCH4III ACNGT 1 cut(s) 58
HpyCH4IV ACGT 4 cut(s) 281, 393, 402, 419
HpyCH4V TGCA 2 cut(s) 411, 584
HpyF10VI GCNNNNNNNGC 7 cut(s) 173, 195, 198, 313, 336, 388, 581
HpyF3I CTNAG 4 cut(s) 12, 78, 340, 519
HpySE526I ACGT 4 cut(s) 281, 393, 402, 419
Hsp92I GRCGYC 1 cut(s) 297
HspAI GCGC 5 cut(s) 122, 132, 174, 297, 330
KasI GGCGCC 1 cut(s) 296
KspI CCGCGG 1 cut(s) 190
Kzo9I GATC 3 cut(s) 287, 366, 459
LmnI GCTCC 6 cut(s) 73, 186, 508, 526, 568, 628
Lsp1109I GCAGC 7 cut(s) 39, 42, 77, 111, 131, 158, 568
LweI GCATC 2 cut(s) 398, 420
MaeI CTAG 2 cut(s) 23, 116
MaeII ACGT 4 cut(s) 281, 393, 402, 419
MaeIII GTNAC 1 cut(s) 479
MalI GATC 3 cut(s) 289, 368, 461
MboI GATC 3 cut(s) 287, 366, 459
MboII GAAGA 4 cut(s) 146, 216, 262, 603
MflI RGATCY 2 cut(s) 287, 459
MhlI GDGCHC 4 cut(s) 363, 393, 446, 531
MluCI AATT 1 cut(s) 634
Mly113I GGCGCC 1 cut(s) 297
MlyI GAGTC 1 cut(s) 104
MmeI TCCRAC 2 cut(s) 78, 385
Mph1103I ATGCAT 1 cut(s) 413
MseI TTAA 1 cut(s) 646
MslI CAYNNNNRTG 1 cut(s) 143
MspA1I CMGCKG 2 cut(s) 171, 189
MspI CCGG 4 cut(s) 195, 291, 500, 572
MspR9I CCNGG 3 cut(s) 178, 195, 542
MvaI CCWGG 2 cut(s) 178, 542
MvnI CGCG 2 cut(s) 189, 353
MwoI GCNNNNNNNGC 7 cut(s) 173, 195, 198, 313, 336, 388, 581
NarI GGCGCC 1 cut(s) 297
NciI CCSGG 1 cut(s) 195
NdeI CATATG 1 cut(s) 140
NdeII GATC 3 cut(s) 287, 366, 459
NlaIV GGNNCC 6 cut(s) 182, 193, 289, 298, 504, 624
NmeAIII GCCGAG 2 cut(s) 197, 325
NmuCI GTSAC 1 cut(s) 479
NsiI ATGCAT 1 cut(s) 413
PcsI WCGNNNNNNNCGW 2 cut(s) 399, 446
PfeI GAWTC 2 cut(s) 373, 515
Pfl23II CGTACG 1 cut(s) 282
PleI GAGTC 1 cut(s) 103
PluTI GGCGCC 1 cut(s) 300
PmaCI CACGTG 2 cut(s) 394, 420
PmlI CACGTG 2 cut(s) 394, 420
PpsI GAGTC 1 cut(s) 103
Ppu21I YACGTR 3 cut(s) 282, 394, 420
PpuMI RGGWCCY 1 cut(s) 256
Psp124BI GAGCTC 1 cut(s) 531
Psp5II RGGWCCY 1 cut(s) 256
Psp6I CCWGG 2 cut(s) 176, 540
PspCI CACGTG 2 cut(s) 394, 420
PspFI CCCAGC 1 cut(s) 316
PspGI CCWGG 2 cut(s) 176, 540
PspLI CGTACG 1 cut(s) 282
PspN4I GGNNCC 6 cut(s) 182, 193, 289, 298, 504, 624
PspPI GGNCC 4 cut(s) 191, 241, 256, 544
PspPPI RGGWCCY 1 cut(s) 256
PsuI RGATCY 2 cut(s) 287, 459
RsaI GTAC 1 cut(s) 284
RsaNI GTAC 1 cut(s) 283
RseI CAYNNNNRTG 1 cut(s) 143
SacI GAGCTC 1 cut(s) 531
SacII CCGCGG 1 cut(s) 190
SaqAI TTAA 1 cut(s) 646
Sau3AI GATC 3 cut(s) 287, 366, 459
Sau96I GGNCC 4 cut(s) 191, 241, 256, 544
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 3 cut(s) 178, 195, 542
SduI GDGCHC 4 cut(s) 363, 393, 446, 531
SfaNI GCATC 2 cut(s) 398, 420
SfoI GGCGCC 1 cut(s) 298
Sfr303I CCGCGG 1 cut(s) 190
SgrBI CCGCGG 1 cut(s) 190
SinI GGWCC 2 cut(s) 256, 544
SmiMI CAYNNNNRTG 1 cut(s) 143
SmlI CTYRAG 2 cut(s) 245, 530
SmoI CTYRAG 2 cut(s) 245, 530
Sse9I AATT 1 cut(s) 634
SspDI GGCGCC 1 cut(s) 296
SspMI CTAG 2 cut(s) 23, 116
SstI GAGCTC 1 cut(s) 531
StyD4I CCNGG 3 cut(s) 176, 193, 540
TaaI ACNGT 1 cut(s) 58
TaiI ACGT 4 cut(s) 284, 396, 405, 422
TaqI TCGA 1 cut(s) 414
TasI AATT 1 cut(s) 634
TfiI GAWTC 2 cut(s) 373, 515
Tru1I TTAA 1 cut(s) 646
Tru9I TTAA 1 cut(s) 646
TscAI CASTG 1 cut(s) 389
TseFI GTSAC 1 cut(s) 479
TseI GCWGC 7 cut(s) 27, 30, 65, 119, 124, 171, 581
Tsp45I GTSAC 1 cut(s) 479
TspDTI ATGAA 1 cut(s) 216
TspGWI ACGGA 1 cut(s) 300
TspRI CASTG 1 cut(s) 389
VpaK11BI GGWCC 2 cut(s) 256, 544
XapI RAATTY 1 cut(s) 634
XspI CTAG 2 cut(s) 23, 116
Zsp2I ATGCAT 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.