FvH4_1g18020

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0008509
Physical Location & Seq
Forward (+)
10489495 .. 10490508
1014 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g18020.t1

Sequence Viewer

Length: 534 bp
ATGGAAGCTGCTTACTCTTCATCCTCTTCAATCACCTCCAAGCAAGCACCACTGCCTTACCACCACTCCTCACTCCACTCCGTCAAGAAAAACTCAGCCAAACCGTGGCGGAAGCCGGCAGTAGCGCCACTCCCTCCGACTCCTCCCCGAGTCTACAAAGTCGACCCCATCAACTTTCGTGACCTTGTCCAGAAGCTCACCAGTGCACCCGAGTACCAGAACGAGCCGACTCGCTCGACTAACCTCCAGAGTGTGGCGCCTCCACCTATCGAAATCCAACGAGGACCGCCACATGTTCCTTCTCCGGTTTCGGCTAAGGTTACCGGCAGCACTTCGCCGTTTTCGGTTATGTACAAGGACTTGGCTGAGACGTTAGGGCTGAGTATTACTCCTCAGCAACAGAAGAAGCCAGTGGATCATCAGAATATCATGGGAGACTCGAGCTATCTGAAGTTGAATTTGTCGCCGGCTTCGCAGCATTGGTTCTCTTTTCCTATGTTGAGCCCTGGAACTCTGACCAGTCTTGAACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VQ

Protein Analysis

178

Amino Acids

19.39

Weight (kDa)

9.58

Isoelectric Point (pI)

60.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 47 - 67 5.7e-08 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 256
AccB7I CCANNNNNTGG 2 cut(s) 105, 253
AccI GTMKAC 2 cut(s) 153, 162
AciI CCGC 2 cut(s) 109, 287
AclWI GGATC 1 cut(s) 423
AcsI RAATTY 1 cut(s) 457
AcuI CTGAAG 1 cut(s) 470
AcyI GRCGYC 1 cut(s) 257
AfaI GTAC 2 cut(s) 215, 353
AfiI CCNNNNNNNGG 2 cut(s) 105, 253
AflIII ACRYGT 1 cut(s) 292
AgsI TTSAA 3 cut(s) 30, 457, 527
AjnI CCWGG 1 cut(s) 505
AluBI AGCT 3 cut(s) 8, 196, 444
AluI AGCT 3 cut(s) 8, 196, 444
Alw21I GWGCWC 1 cut(s) 208
Alw26I GTCTC 2 cut(s) 362, 429
Alw44I GTGCAC 1 cut(s) 204
AlwI GGATC 1 cut(s) 423
Ama87I CYCGRG 3 cut(s) 147, 209, 439
ApaLI GTGCAC 1 cut(s) 204
ApeKI GCWGC 3 cut(s) 8, 327, 475
ApoI RAATTY 1 cut(s) 457
AspLEI GCGC 2 cut(s) 127, 259
AspS9I GGNCC 1 cut(s) 284
AsuHPI GGTGA 2 cut(s) 25, 190
AvaI CYCGRG 3 cut(s) 147, 209, 439
AvaII GGWCC 1 cut(s) 284
BaeGI GKGCMC 1 cut(s) 208
BaeI ACNNNNGTAYC 2 cut(s) 197, 230
BanI GGYRCC 1 cut(s) 256
BanII GRGCYC 1 cut(s) 506
Bbv12I GWGCWC 1 cut(s) 208
BbvCI CCTCAGC 1 cut(s) 393
BbvI GCAGC 2 cut(s) 339, 487
BccI CCATC 1 cut(s) 176
BceAI ACGGC 1 cut(s) 322
BciT130I CCWGG 1 cut(s) 507
BcoDI GTCTC 2 cut(s) 362, 429
BfaI CTAG 1 cut(s) 532
BfoI RGCGCY 2 cut(s) 128, 260
BisI GCNGC 3 cut(s) 9, 328, 476
BlsI GCNGC 3 cut(s) 10, 329, 477
Bme1390I CCNGG 1 cut(s) 507
Bme18I GGWCC 1 cut(s) 284
BmeT110I CYCGRG 3 cut(s) 147, 209, 439
BmgT120I GGNCC 1 cut(s) 284
BmiI GGNNCC 1 cut(s) 258
BmrFI CCNGG 1 cut(s) 507
BplI GAGNNNNNCTC 2 cut(s) 373, 405
BpmI CTGGAG 1 cut(s) 230
Bpu10I CCTNAGC 2 cut(s) 315, 393
BsaHI GRCGYC 1 cut(s) 257
BsaJI CCNNGG 2 cut(s) 104, 505
BsaWI WCCGGW 1 cut(s) 304
BsaXI ACNNNNNCTCC 2 cut(s) 50, 80
Bsc4I CCNNNNNNNGG 2 cut(s) 105, 253
Bse118I RCCGGY 3 cut(s) 115, 323, 466
Bse1I ACTGG 3 cut(s) 201, 410, 519
BseBI CCWGG 1 cut(s) 507
BseDI CCNNGG 2 cut(s) 104, 505
BseGI GGATG 1 cut(s) 20
BseLI CCNNNNNNNGG 2 cut(s) 105, 253
BseMII CTCAG 4 cut(s) 108, 357, 371, 407
BseNI ACTGG 3 cut(s) 201, 410, 519
BseRI GAGGAG 3 cut(s) 58, 132, 381
BseSI GKGCMC 1 cut(s) 208
BseXI GCAGC 2 cut(s) 339, 487
BshNI GGYRCC 1 cut(s) 256
BsiHKAI GWGCWC 1 cut(s) 208
BsiHKCI CYCGRG 3 cut(s) 147, 209, 439
BsiSI CCGG 4 cut(s) 116, 305, 324, 467
BslI CCNNNNNNNGG 2 cut(s) 105, 253
BsmAI GTCTC 2 cut(s) 362, 429
BsmBI CGTCTC 1 cut(s) 362
BsoBI CYCGRG 3 cut(s) 147, 209, 439
Bsp1286I GDGCHC 2 cut(s) 208, 506
Bsp1407I TGTACA 1 cut(s) 351
Bsp143I GATC 1 cut(s) 415
BspACI CCGC 2 cut(s) 109, 287
BspCNI CTCAG 4 cut(s) 107, 358, 372, 406
BspLI GGNNCC 1 cut(s) 258
BspPI GGATC 1 cut(s) 423
BspT107I GGYRCC 1 cut(s) 256
BsrFI RCCGGY 3 cut(s) 115, 323, 466
BsrGI TGTACA 1 cut(s) 351
BsrI ACTGG 3 cut(s) 201, 410, 519
BssAI RCCGGY 3 cut(s) 115, 323, 466
BssECI CCNNGG 2 cut(s) 104, 505
BssMI GATC 1 cut(s) 415
BssNI GRCGYC 1 cut(s) 257
Bst2UI CCWGG 1 cut(s) 507
Bst4CI ACNGT 1 cut(s) 105
Bst6I CTCTTC 2 cut(s) 22, 31
BstACI GRCGYC 1 cut(s) 257
BstAUI TGTACA 1 cut(s) 351
BstC8I GCNNGC 3 cut(s) 45, 117, 468
BstDEI CTNAG 5 cut(s) 94, 315, 366, 380, 393
BstDSI CCRYGG 1 cut(s) 104
BstEII GGTNACC 1 cut(s) 319
BstF5I GGATG 1 cut(s) 20
BstH2I RGCGCY 2 cut(s) 128, 260
BstHHI GCGC 2 cut(s) 127, 259
BstKTI GATC 1 cut(s) 418
BstMAI GTCTC 2 cut(s) 362, 429
BstMBI GATC 1 cut(s) 415
BstMWI GCNNNNNNNGC 1 cut(s) 472
BstNI CCWGG 1 cut(s) 507
BstNSI RCATGY 1 cut(s) 296
BstPI GGTNACC 1 cut(s) 319
BstSCI CCNGG 1 cut(s) 505
BstSLI GKGCMC 1 cut(s) 208
BstV1I GCAGC 2 cut(s) 339, 487
BtgI CCRYGG 1 cut(s) 104
BtsCI GGATG 1 cut(s) 20
BtsI GCAGTG 1 cut(s) 50
BtsIMutI CAGTG 3 cut(s) 50, 208, 417
Cac8I GCNNGC 3 cut(s) 45, 117, 468
CfoI GCGC 2 cut(s) 127, 259
Cfr10I RCCGGY 3 cut(s) 115, 323, 466
Cfr13I GGNCC 1 cut(s) 284
Csp6I GTAC 2 cut(s) 214, 352
CviAII CATG 2 cut(s) 293, 430
CviQI GTAC 2 cut(s) 214, 352
DdeI CTNAG 5 cut(s) 94, 315, 366, 380, 393
DinI GGCGCC 1 cut(s) 258
DpnI GATC 1 cut(s) 417
DpnII GATC 1 cut(s) 415
Eam1104I CTCTTC 2 cut(s) 22, 31
EarI CTCTTC 2 cut(s) 22, 31
EciI GGCGGA 1 cut(s) 124
Eco24I GRGCYC 1 cut(s) 506
Eco47I GGWCC 1 cut(s) 284
Eco57I CTGAAG 1 cut(s) 470
Eco88I CYCGRG 3 cut(s) 147, 209, 439
Eco91I GGTNACC 1 cut(s) 319
EcoO65I GGTNACC 1 cut(s) 319
EcoRII CCWGG 1 cut(s) 505
EcoT38I GRGCYC 1 cut(s) 506
EgeI GGCGCC 1 cut(s) 258
EheI GGCGCC 1 cut(s) 258
Esp3I CGTCTC 1 cut(s) 362
FaeI CATG 2 cut(s) 296, 433
FaiI YATR 4 cut(s) 294, 350, 431, 497
FatI CATG 2 cut(s) 292, 429
FblI GTMKAC 2 cut(s) 153, 162
Fnu4HI GCNGC 3 cut(s) 9, 328, 476
FokI GGATG 1 cut(s) 7
FriOI GRGCYC 1 cut(s) 506
Fsp4HI GCNGC 3 cut(s) 9, 328, 476
FspBI CTAG 1 cut(s) 532
GlaI GCGC 2 cut(s) 126, 258
GluI GCNGC 3 cut(s) 9, 328, 476
GsuI CTGGAG 1 cut(s) 230
HaeII RGCGCY 2 cut(s) 128, 260
HapII CCGG 4 cut(s) 116, 305, 324, 467
HhaI GCGC 2 cut(s) 127, 259
Hin1I GRCGYC 1 cut(s) 257
Hin1II CATG 2 cut(s) 296, 433
Hin6I GCGC 2 cut(s) 125, 257
HinP1I GCGC 2 cut(s) 125, 257
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 4 cut(s) 139, 150, 229, 437
HpaII CCGG 4 cut(s) 116, 305, 324, 467
HphI GGTGA 2 cut(s) 25, 190
Hpy166II GTNNAC 3 cut(s) 154, 163, 206
Hpy188I TCNGA 4 cut(s) 138, 423, 450, 516
Hpy188III TCNNGA 5 cut(s) 85, 179, 190, 247, 524
Hpy8I GTNNAC 3 cut(s) 154, 163, 206
HpyAV CCTTC 1 cut(s) 309
HpyCH4III ACNGT 1 cut(s) 105
HpyCH4IV ACGT 1 cut(s) 371
HpyCH4V TGCA 1 cut(s) 206
HpyF10VI GCNNNNNNNGC 1 cut(s) 472
HpyF3I CTNAG 5 cut(s) 94, 315, 366, 380, 393
HpySE526I ACGT 1 cut(s) 371
Hsp92I GRCGYC 1 cut(s) 257
Hsp92II CATG 2 cut(s) 296, 433
HspAI GCGC 2 cut(s) 125, 257
KasI GGCGCC 1 cut(s) 256
KroI GCCGGC 2 cut(s) 115, 466
KroNI GCCGGC 2 cut(s) 117, 468
Kzo9I GATC 1 cut(s) 415
Lsp1109I GCAGC 2 cut(s) 339, 487
MaeI CTAG 1 cut(s) 532
MaeII ACGT 1 cut(s) 371
MaeIII GTNAC 2 cut(s) 179, 319
MalI GATC 1 cut(s) 417
MboI GATC 1 cut(s) 415
MboII GAAGA 3 cut(s) 9, 18, 415
MhlI GDGCHC 2 cut(s) 208, 506
MluCI AATT 1 cut(s) 457
Mly113I GGCGCC 1 cut(s) 257
MlyI GAGTC 4 cut(s) 133, 159, 223, 431
MmeI TCCRAC 2 cut(s) 161, 301
MnlI CCTC 9 cut(s) 34, 46, 79, 144, 153, 254, 270, 275, 402
MroNI GCCGGC 2 cut(s) 115, 466
MspI CCGG 4 cut(s) 116, 305, 324, 467
MspR9I CCNGG 1 cut(s) 507
MvaI CCWGG 1 cut(s) 507
MwoI GCNNNNNNNGC 1 cut(s) 472
NaeI GCCGGC 2 cut(s) 117, 468
NarI GGCGCC 1 cut(s) 257
NdeII GATC 1 cut(s) 415
NgoMIV GCCGGC 2 cut(s) 115, 466
NlaIII CATG 2 cut(s) 296, 433
NlaIV GGNNCC 1 cut(s) 258
NmuCI GTSAC 1 cut(s) 179
NspI RCATGY 1 cut(s) 296
PaeR7I CTCGAG 1 cut(s) 439
PciI ACATGT 1 cut(s) 292
PdiI GCCGGC 2 cut(s) 117, 468
PflFI GACNNNGTC 1 cut(s) 185
PflMI CCANNNNNTGG 2 cut(s) 105, 253
PkrI GCNGC 3 cut(s) 10, 329, 477
PleI GAGTC 4 cut(s) 133, 158, 223, 431
PluTI GGCGCC 1 cut(s) 260
PpsI GAGTC 4 cut(s) 133, 158, 223, 431
PscI ACATGT 1 cut(s) 292
Psp6I CCWGG 1 cut(s) 505
PspEI GGTNACC 1 cut(s) 319
PspGI CCWGG 1 cut(s) 505
PspN4I GGNNCC 1 cut(s) 258
PspPI GGNCC 1 cut(s) 284
PspXI VCTCGAGB 1 cut(s) 439
PsyI GACNNNGTC 1 cut(s) 185
RsaI GTAC 2 cut(s) 215, 353
RsaNI GTAC 2 cut(s) 214, 352
SalI GTCGAC 1 cut(s) 161
SatI GCNGC 3 cut(s) 9, 328, 476
Sau3AI GATC 1 cut(s) 415
Sau96I GGNCC 1 cut(s) 284
SchI GAGTC 4 cut(s) 133, 159, 223, 431
ScrFI CCNGG 1 cut(s) 507
SduI GDGCHC 2 cut(s) 208, 506
SetI ASST 9 cut(s) 10, 38, 186, 198, 246, 268, 321, 374, 446
SfoI GGCGCC 1 cut(s) 258
Sfr274I CTCGAG 1 cut(s) 439
SinI GGWCC 1 cut(s) 284
SlaI CTCGAG 1 cut(s) 439
SmlI CTYRAG 1 cut(s) 439
SmoI CTYRAG 1 cut(s) 439
Sse9I AATT 1 cut(s) 457
SsiI CCGC 2 cut(s) 109, 287
SspDI GGCGCC 1 cut(s) 256
SspMI CTAG 1 cut(s) 532
StyD4I CCNGG 1 cut(s) 505
TaaI ACNGT 1 cut(s) 105
TaiI ACGT 1 cut(s) 374
TaqI TCGA 4 cut(s) 162, 236, 270, 440
TasI AATT 1 cut(s) 457
TatI WGTACW 1 cut(s) 351
TscAI CASTG 3 cut(s) 57, 208, 417
TseFI GTSAC 1 cut(s) 179
TseI GCWGC 3 cut(s) 8, 327, 475
Tsp45I GTSAC 1 cut(s) 179
TspDTI ATGAA 1 cut(s) 9
TspGWI ACGGA 1 cut(s) 70
TspRI CASTG 3 cut(s) 57, 208, 417
Tth111I GACNNNGTC 1 cut(s) 185
Van91I CCANNNNNTGG 2 cut(s) 105, 253
VneI GTGCAC 1 cut(s) 204
VpaK11BI GGWCC 1 cut(s) 284
XapI RAATTY 1 cut(s) 457
XceI RCATGY 1 cut(s) 296
XhoI CTCGAG 1 cut(s) 439
XmiI GTMKAC 2 cut(s) 153, 162
XspI CTAG 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.