pycom02g15290

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0008509
Physical Location & Seq
Forward (+)
12034593 .. 12035159
567 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g15290.1

Sequence Viewer

Length: 567 bp
ATGGAATCTTACGCCTCTTCCTCTTCAGCCTCCTCCACCACCACATACTTGTCCCAAACCAAACAACCAACAAAGGGTTCAATTTCAACCTCAAAGCAGCTTCCCAATCACCAGTCAGCACTCCACTCGGCTCGAAAATCTTCGTCGAAGCCATGGAAGAAACCTGCTGTTGCACCACTTCCACCAACCCCACCAAGAATCTACAAAGTTGACCCCATTAACTTCAGGGAGCTTGTCCAGAGCCTAACCAGCGAGCCAGAGTTTATGGAGACTCGGTCTCTCCAGAGCGTAGCTCCTCCACCGATCGATATCCGTGTGTCTTCTGTACCAAACCATGCCCCTTCTTCTTCACCAAAAATTAGCTCACCATTTTCGGTTATGTACAAGGAATTGTCGGATACATTGGAGCTGAGTCCGATGCCTCGACAACAGAAGGTGCATGATAGCACGGTGGACTCGAGTTACCTGAGACTGAATCTGCAGCCCCCGTCTTCCCATCATTGGTTTTCTTTCCCTATGCTCAGCCCAGGGACTTTGTCCAGTATCGAACGACCCGAAGTTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VQ

Protein Analysis

189

Amino Acids

20.74

Weight (kDa)

9.52

Isoelectric Point (pI)

77.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 63 - 83 6.5e-07 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 172
AcuI CTGAAG 2 cut(s) 9, 208
AfaI GTAC 2 cut(s) 327, 383
AfiI CCNNNNNNNGG 2 cut(s) 74, 501
AgsI TTSAA 2 cut(s) 81, 87
AjnI CCWGG 1 cut(s) 526
AjuI GAANNNNNNNTTGG 2 cut(s) 61, 93
AluBI AGCT 5 cut(s) 100, 232, 293, 363, 409
AluI AGCT 5 cut(s) 100, 232, 293, 363, 409
Alw26I GTCTC 3 cut(s) 263, 282, 463
Ama87I CYCGRG 1 cut(s) 457
ApeKI GCWGC 2 cut(s) 97, 481
Asp700I GAANNNNTTC 1 cut(s) 139
AsuHPI GGTGA 3 cut(s) 101, 342, 357
AvaI CYCGRG 1 cut(s) 457
BbsI GAAGAC 2 cut(s) 312, 483
BbvI GCAGC 2 cut(s) 109, 493
BccI CCATC 1 cut(s) 504
BcgI CGANNNNNNTGC 2 cut(s) 108, 142
BciT130I CCWGG 1 cut(s) 528
BciVI GTATCC 1 cut(s) 391
BcoDI GTCTC 3 cut(s) 263, 282, 463
BfmI CTRYAG 1 cut(s) 479
BfuAI ACCTGC 1 cut(s) 172
BfuI GTATCC 1 cut(s) 391
BisI GCNGC 2 cut(s) 98, 482
BlpI GCTNAGC 1 cut(s) 521
BlsI GCNGC 2 cut(s) 99, 483
Bme1390I CCNGG 1 cut(s) 528
BmeT110I CYCGRG 1 cut(s) 457
BmrFI CCNGG 1 cut(s) 528
BmsI GCATC 1 cut(s) 408
BpiI GAAGAC 2 cut(s) 312, 483
BplI GAGNNNNNCTC 2 cut(s) 277, 309
BpmI CTGGAG 1 cut(s) 266
Bpu1102I GCTNAGC 1 cut(s) 521
Bsa29I ATCGAT 1 cut(s) 306
BsaBI GATNNNNATC 1 cut(s) 308
BsaI GGTCTC 1 cut(s) 282
BsaJI CCNNGG 3 cut(s) 152, 526, 527
BsaXI ACNNNNNCTCC 2 cut(s) 260, 290
Bsc4I CCNNNNNNNGG 2 cut(s) 74, 501
Bse1I ACTGG 2 cut(s) 112, 540
Bse8I GATNNNNATC 1 cut(s) 308
BseBI CCWGG 1 cut(s) 528
BseCI ATCGAT 1 cut(s) 306
BseDI CCNNGG 3 cut(s) 152, 526, 527
BseJI GATNNNNATC 1 cut(s) 308
BseLI CCNNNNNNNGG 2 cut(s) 74, 501
BseMII CTCAG 3 cut(s) 401, 458, 535
BseNI ACTGG 2 cut(s) 112, 540
BseRI GAGGAG 2 cut(s) 22, 285
BseXI GCAGC 2 cut(s) 109, 493
Bsh1285I CGRYCG 1 cut(s) 306
BshVI ATCGAT 1 cut(s) 306
BsiEI CGRYCG 1 cut(s) 306
BsiHKCI CYCGRG 1 cut(s) 457
BslFI GGGAC 2 cut(s) 37, 544
BslI CCNNNNNNNGG 2 cut(s) 74, 501
BsmAI GTCTC 3 cut(s) 263, 282, 463
BsmFI GGGAC 2 cut(s) 37, 544
Bso31I GGTCTC 1 cut(s) 282
BsoBI CYCGRG 1 cut(s) 457
Bsp1407I TGTACA 1 cut(s) 381
Bsp143I GATC 1 cut(s) 303
Bsp1720I GCTNAGC 1 cut(s) 521
Bsp19I CCATGG 1 cut(s) 152
BspCNI CTCAG 3 cut(s) 402, 459, 534
BspDI ATCGAT 1 cut(s) 306
BspMAI CTGCAG 1 cut(s) 483
BspMI ACCTGC 1 cut(s) 172
BspTNI GGTCTC 1 cut(s) 282
BsrGI TGTACA 1 cut(s) 381
BsrI ACTGG 2 cut(s) 112, 540
BssECI CCNNGG 3 cut(s) 152, 526, 527
BssMI GATC 1 cut(s) 303
BssT1I CCWWGG 1 cut(s) 152
Bst2UI CCWGG 1 cut(s) 528
Bst4CI ACNGT 1 cut(s) 451
Bst6I CTCTTC 2 cut(s) 22, 28
BstAUI TGTACA 1 cut(s) 381
BstC8I GCNNGC 1 cut(s) 254
BstDEI CTNAG 3 cut(s) 410, 467, 521
BstDSI CCRYGG 1 cut(s) 152
BstKTI GATC 1 cut(s) 306
BstMAI GTCTC 3 cut(s) 263, 282, 463
BstMBI GATC 1 cut(s) 303
BstMCI CGRYCG 1 cut(s) 306
BstMWI GCNNNNNNNGC 1 cut(s) 249
BstNI CCWGG 1 cut(s) 528
BstSCI CCNGG 1 cut(s) 526
BstSFI CTRYAG 1 cut(s) 479
BstV1I GCAGC 2 cut(s) 109, 493
BstV2I GAAGAC 2 cut(s) 312, 483
Bsu15I ATCGAT 1 cut(s) 306
BsuI GTATCC 1 cut(s) 391
BsuTUI ATCGAT 1 cut(s) 306
BtgI CCRYGG 1 cut(s) 152
BveI ACCTGC 1 cut(s) 172
Cac8I GCNNGC 1 cut(s) 254
ClaI ATCGAT 1 cut(s) 306
Csp6I GTAC 2 cut(s) 326, 382
CviAII CATG 3 cut(s) 153, 335, 440
CviQI GTAC 2 cut(s) 326, 382
DdeI CTNAG 3 cut(s) 410, 467, 521
DpnI GATC 1 cut(s) 305
DpnII GATC 1 cut(s) 303
Eam1104I CTCTTC 2 cut(s) 22, 28
EarI CTCTTC 2 cut(s) 22, 28
Eco130I CCWWGG 1 cut(s) 152
Eco31I GGTCTC 1 cut(s) 282
Eco32I GATATC 1 cut(s) 310
Eco57I CTGAAG 2 cut(s) 9, 208
Eco88I CYCGRG 1 cut(s) 457
EcoRII CCWGG 1 cut(s) 526
EcoRV GATATC 1 cut(s) 310
EcoT14I CCWWGG 1 cut(s) 152
ErhI CCWWGG 1 cut(s) 152
FaeI CATG 3 cut(s) 156, 338, 443
FaiI YATR 7 cut(s) 46, 154, 266, 336, 380, 441, 518
FaqI GGGAC 2 cut(s) 37, 544
FatI CATG 3 cut(s) 152, 334, 439
Fnu4HI GCNGC 2 cut(s) 98, 482
Fsp4HI GCNGC 2 cut(s) 98, 482
GluI GCNGC 2 cut(s) 98, 482
GsuI CTGGAG 1 cut(s) 266
Hin1II CATG 3 cut(s) 156, 338, 443
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HinfI GANTC 6 cut(s) 5, 198, 271, 412, 455, 475
HphI GGTGA 3 cut(s) 101, 342, 357
Hpy166II GTNNAC 2 cut(s) 211, 454
Hpy188I TCNGA 2 cut(s) 397, 417
Hpy188III TCNNGA 2 cut(s) 238, 283
Hpy8I GTNNAC 2 cut(s) 211, 454
Hpy99I CGWCG 1 cut(s) 148
HpyAV CCTTC 2 cut(s) 351, 427
HpyCH4III ACNGT 1 cut(s) 451
HpyCH4V TGCA 3 cut(s) 173, 439, 481
HpyF10VI GCNNNNNNNGC 1 cut(s) 249
HpyF3I CTNAG 3 cut(s) 410, 467, 521
Hsp92II CATG 3 cut(s) 156, 338, 443
Kzo9I GATC 1 cut(s) 303
LmnI GCTCC 3 cut(s) 229, 298, 406
Lsp1109I GCAGC 2 cut(s) 109, 493
LweI GCATC 1 cut(s) 408
MaeIII GTNAC 1 cut(s) 461
MalI GATC 1 cut(s) 305
MboI GATC 1 cut(s) 303
MboII GAAGA 8 cut(s) 9, 15, 132, 169, 312, 336, 339, 483
MluCI AATT 3 cut(s) 81, 357, 389
MlyI GAGTC 3 cut(s) 265, 421, 449
MmeI TCCRAC 1 cut(s) 375
MnlI CCTC 7 cut(s) 25, 31, 40, 43, 100, 306, 432
MroXI GAANNNNTTC 1 cut(s) 139
MseI TTAA 1 cut(s) 219
MspR9I CCNGG 1 cut(s) 528
MvaI CCWGG 1 cut(s) 528
MwoI GCNNNNNNNGC 1 cut(s) 249
NcoI CCATGG 1 cut(s) 152
NdeII GATC 1 cut(s) 303
NlaIII CATG 3 cut(s) 156, 338, 443
NmeAIII GCCGAG 1 cut(s) 107
PaeR7I CTCGAG 1 cut(s) 457
PasI CCCWGGG 1 cut(s) 527
PcsI WCGNNNNNNNCGW 2 cut(s) 455, 552
PdmI GAANNNNTTC 1 cut(s) 139
PfeI GAWTC 3 cut(s) 5, 198, 475
PflFI GACNNNGTC 2 cut(s) 274, 535
PkrI GCNGC 2 cut(s) 99, 483
Ple19I CGATCG 1 cut(s) 306
PleI GAGTC 3 cut(s) 265, 420, 449
PpsI GAGTC 3 cut(s) 265, 420, 449
Psp6I CCWGG 1 cut(s) 526
PspGI CCWGG 1 cut(s) 526
PspXI VCTCGAGB 1 cut(s) 457
PstI CTGCAG 1 cut(s) 483
PsyI GACNNNGTC 2 cut(s) 274, 535
PvuI CGATCG 1 cut(s) 306
RsaI GTAC 2 cut(s) 327, 383
RsaNI GTAC 2 cut(s) 326, 382
SaqAI TTAA 1 cut(s) 219
SatI GCNGC 2 cut(s) 98, 482
Sau3AI GATC 1 cut(s) 303
SchI GAGTC 3 cut(s) 265, 421, 449
ScrFI CCNGG 1 cut(s) 528
SetI ASST 9 cut(s) 92, 102, 166, 234, 295, 365, 411, 438, 468
SfaNI GCATC 1 cut(s) 408
SfcI CTRYAG 1 cut(s) 479
Sfr274I CTCGAG 1 cut(s) 457
SlaI CTCGAG 1 cut(s) 457
SmlI CTYRAG 1 cut(s) 457
SmoI CTYRAG 1 cut(s) 457
Sse9I AATT 3 cut(s) 81, 357, 389
StyD4I CCNGG 1 cut(s) 526
StyI CCWWGG 1 cut(s) 152
TaaI ACNGT 1 cut(s) 451
TaqI TCGA 6 cut(s) 133, 146, 306, 424, 458, 546
TaqII GACCGA 1 cut(s) 264
TasI AATT 3 cut(s) 81, 357, 389
TatI WGTACW 1 cut(s) 381
TfiI GAWTC 3 cut(s) 5, 198, 475
Tru1I TTAA 1 cut(s) 219
Tru9I TTAA 1 cut(s) 219
TseI GCWGC 2 cut(s) 97, 481
TspGWI ACGGA 1 cut(s) 302
Tth111I GACNNNGTC 2 cut(s) 274, 535
XhoI CTCGAG 1 cut(s) 457
XmnI GAANNNNTTC 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.