FvH4_1g22170

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0007424
Physical Location & Seq
Forward (+)
14121189 .. 14123897
2709 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g22170.t2

Sequence Viewer

Length: 573 bp
ATGTCGTTCGCTCTCAAATTGAATCTCTCAAACCCTAGAATCAAACCCAACTCCAACTTCGCCGGTAACGCCGTCATTCCCCACCGTCTCAGCCTCCCGGCACGGCGGCTCAACTCCCTTTCCGTCCACTCACAGTACGTTGACGGCGCATCCGAGCCGTCAATTTCATCGCATTACACCGAAACCATCGGAACCCCATCGCCGTCGTCTCAGCTTCTCCAGTGGAACCTCACCCAACGACACGTCGTCGTCCTCAATGTCGTTGCTTGCGCCGCGGCGGTTTCTGCTACGTGGCTCTTCTGCTCTGCAATTCCAACGCTTCTGGCTTTCAAGAAGGCAGCTGATTCACTTGCGAAGCTGATGGATGTGATGAGGGAAGAGCTTCCGGATACAATGGCGGCTGTTCGTTTATCCGGGATGGAGATCAGTGACCTCACCATGGAGCTCAGTGATCTCGGACAGGAGATTACGCAAGGTGTGAGGAGTTCTACTCGGGCTGTTCGTGTGGCAGAGGAGAGGTTGCGCGGCTTGACTAACATGGCTCCTTCAGGTAGTAGAAAGTCCGAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

191

Amino Acids

20.59

Weight (kDa)

9.1

Isoelectric Point (pI)

44.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 275, 525
AccIII TCCGGA 1 cut(s) 385
AciI CCGC 6 cut(s) 106, 273, 275, 278, 398, 525
AcuI CTGAAG 1 cut(s) 531
AfaI GTAC 1 cut(s) 137
AfiI CCNNNNNNNGG 1 cut(s) 439
AflIII ACRYGT 1 cut(s) 241
AgsI TTSAA 2 cut(s) 22, 331
AhdI GACNNNNNGTC 1 cut(s) 245
AjiI CACGTC 1 cut(s) 244
AjuI GAANNNNNNNTTGG 2 cut(s) 41, 73
AluBI AGCT 5 cut(s) 214, 341, 358, 382, 445
AluI AGCT 5 cut(s) 214, 341, 358, 382, 445
Alw21I GWGCWC 1 cut(s) 447
Alw26I GTCTC 2 cut(s) 92, 213
Ama87I CYCGRG 1 cut(s) 492
Aor13HI TCCGGA 1 cut(s) 385
ApeKI GCWGC 1 cut(s) 338
Asp700I GAANNNNTTC 1 cut(s) 381
AspLEI GCGC 3 cut(s) 149, 272, 525
AsuC2I CCSGG 2 cut(s) 98, 415
AsuHPI GGTGA 2 cut(s) 223, 427
AvaI CYCGRG 1 cut(s) 492
BanII GRGCYC 1 cut(s) 447
Bbv12I GWGCWC 1 cut(s) 447
BbvI GCAGC 1 cut(s) 350
BccI CCATC 4 cut(s) 194, 205, 355, 412
BceAI ACGGC 5 cut(s) 56, 119, 142, 160, 187
BciVI GTATCC 1 cut(s) 382
BcnI CCSGG 2 cut(s) 98, 415
BcoDI GTCTC 2 cut(s) 92, 213
BfaI CTAG 1 cut(s) 36
BfuI GTATCC 1 cut(s) 382
BisI GCNGC 6 cut(s) 107, 273, 276, 339, 399, 526
BlsI GCNGC 6 cut(s) 108, 274, 277, 340, 400, 527
Bme1390I CCNGG 2 cut(s) 98, 415
BmeRI GACNNNNNGTC 1 cut(s) 245
BmeT110I CYCGRG 1 cut(s) 492
BmgBI CACGTC 1 cut(s) 244
BmiI GGNNCC 3 cut(s) 193, 227, 543
BmrFI CCNGG 2 cut(s) 98, 415
BmsI GCATC 1 cut(s) 158
BplI GAGNNNNNCTC 2 cut(s) 475, 507
BpmI CTGGAG 1 cut(s) 203
BpuMI CCSGG 2 cut(s) 98, 415
BsaAI YACGTR 1 cut(s) 291
BsaBI GATNNNNATC 1 cut(s) 422
BsaJI CCNNGG 2 cut(s) 273, 438
BsaWI WCCGGW 1 cut(s) 385
Bsc4I CCNNNNNNNGG 1 cut(s) 439
Bse118I RCCGGY 1 cut(s) 62
Bse1I ACTGG 1 cut(s) 220
Bse8I GATNNNNATC 1 cut(s) 422
BseAI TCCGGA 1 cut(s) 385
BseDI CCNNGG 2 cut(s) 273, 438
BseGI GGATG 3 cut(s) 149, 370, 423
BseJI GATNNNNATC 1 cut(s) 422
BseLI CCNNNNNNNGG 1 cut(s) 439
BseMII CTCAG 3 cut(s) 103, 224, 460
BseNI ACTGG 1 cut(s) 220
BseRI GAGGAG 2 cut(s) 496, 527
BseXI GCAGC 1 cut(s) 350
Bsh1236I CGCG 2 cut(s) 275, 525
BsiHKAI GWGCWC 1 cut(s) 447
BsiHKCI CYCGRG 1 cut(s) 492
BsiSI CCGG 4 cut(s) 63, 98, 386, 414
BslI CCNNNNNNNGG 1 cut(s) 439
BsmAI GTCTC 2 cut(s) 92, 213
BsmBI CGTCTC 2 cut(s) 92, 213
BsoBI CYCGRG 1 cut(s) 492
Bsp1286I GDGCHC 1 cut(s) 447
Bsp13I TCCGGA 1 cut(s) 385
Bsp143I GATC 2 cut(s) 423, 451
Bsp19I CCATGG 1 cut(s) 438
BspACI CCGC 6 cut(s) 106, 273, 275, 278, 398, 525
BspCNI CTCAG 3 cut(s) 102, 223, 459
BspEI TCCGGA 1 cut(s) 385
BspFNI CGCG 2 cut(s) 275, 525
BspLI GGNNCC 3 cut(s) 193, 227, 543
BspQI GCTCTTC 2 cut(s) 302, 372
BsrFI RCCGGY 1 cut(s) 62
BsrI ACTGG 1 cut(s) 220
BssAI RCCGGY 1 cut(s) 62
BssECI CCNNGG 2 cut(s) 273, 438
BssMI GATC 2 cut(s) 423, 451
BssT1I CCWWGG 1 cut(s) 438
Bst4CI ACNGT 2 cut(s) 86, 135
Bst6I CTCTTC 2 cut(s) 302, 372
BstBAI YACGTR 1 cut(s) 291
BstC8I GCNNGC 1 cut(s) 268
BstDEI CTNAG 3 cut(s) 89, 210, 446
BstDSI CCRYGG 2 cut(s) 273, 438
BstF5I GGATG 3 cut(s) 149, 370, 423
BstFNI CGCG 2 cut(s) 275, 525
BstHHI GCGC 3 cut(s) 149, 272, 525
BstKTI GATC 2 cut(s) 426, 454
BstMAI GTCTC 2 cut(s) 92, 213
BstMBI GATC 2 cut(s) 423, 451
BstMWI GCNNNNNNNGC 3 cut(s) 68, 272, 284
BstSCI CCNGG 2 cut(s) 96, 413
BstUI CGCG 2 cut(s) 275, 525
BstV1I GCAGC 1 cut(s) 350
BsuI GTATCC 1 cut(s) 382
BtgI CCRYGG 2 cut(s) 273, 438
BtgZI GCGATG 2 cut(s) 153, 183
BtrI CACGTC 1 cut(s) 244
BtsCI GGATG 3 cut(s) 149, 370, 423
BtsIMutI CAGTG 3 cut(s) 227, 433, 454
Cac8I GCNNGC 1 cut(s) 268
CfoI GCGC 3 cut(s) 149, 272, 525
Cfr10I RCCGGY 1 cut(s) 62
Cfr42I CCGCGG 1 cut(s) 276
Csp6I GTAC 1 cut(s) 136
CviAII CATG 2 cut(s) 439, 538
CviQI GTAC 1 cut(s) 136
DdeI CTNAG 3 cut(s) 89, 210, 446
DpnI GATC 2 cut(s) 425, 453
DpnII GATC 2 cut(s) 423, 451
DriI GACNNNNNGTC 1 cut(s) 245
Eam1104I CTCTTC 2 cut(s) 302, 372
Eam1105I GACNNNNNGTC 1 cut(s) 245
EarI CTCTTC 2 cut(s) 302, 372
Ecl136II GAGCTC 1 cut(s) 445
Eco130I CCWWGG 1 cut(s) 438
Eco24I GRGCYC 1 cut(s) 447
Eco53kI GAGCTC 1 cut(s) 445
Eco57I CTGAAG 1 cut(s) 531
Eco88I CYCGRG 1 cut(s) 492
EcoICRI GAGCTC 1 cut(s) 445
EcoT14I CCWWGG 1 cut(s) 438
EcoT38I GRGCYC 1 cut(s) 447
ErhI CCWWGG 1 cut(s) 438
Esp3I CGTCTC 2 cut(s) 92, 213
FaeI CATG 2 cut(s) 442, 541
FaiI YATR 2 cut(s) 440, 539
FatI CATG 2 cut(s) 438, 537
Fnu4HI GCNGC 6 cut(s) 107, 273, 276, 339, 399, 526
FokI GGATG 3 cut(s) 136, 377, 430
FriOI GRGCYC 1 cut(s) 447
Fsp4HI GCNGC 6 cut(s) 107, 273, 276, 339, 399, 526
FspBI CTAG 1 cut(s) 36
GlaI GCGC 3 cut(s) 148, 271, 524
GluI GCNGC 6 cut(s) 107, 273, 276, 339, 399, 526
GsuI CTGGAG 1 cut(s) 203
HapII CCGG 4 cut(s) 63, 98, 386, 414
HhaI GCGC 3 cut(s) 149, 272, 525
Hin1II CATG 2 cut(s) 442, 541
Hin6I GCGC 3 cut(s) 147, 270, 523
HinP1I GCGC 3 cut(s) 147, 270, 523
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HinfI GANTC 3 cut(s) 22, 39, 344
HpaII CCGG 4 cut(s) 63, 98, 386, 414
HphI GGTGA 2 cut(s) 223, 427
Hpy166II GTNNAC 2 cut(s) 127, 142
Hpy188I TCNGA 4 cut(s) 154, 191, 458, 565
Hpy188III TCNNGA 2 cut(s) 331, 386
Hpy8I GTNNAC 2 cut(s) 127, 142
Hpy99I CGWCG 3 cut(s) 208, 248, 251
HpyAV CCTTC 2 cut(s) 328, 555
HpyCH4III ACNGT 2 cut(s) 86, 135
HpyCH4IV ACGT 3 cut(s) 138, 243, 290
HpyCH4V TGCA 1 cut(s) 308
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 272, 284
HpyF3I CTNAG 3 cut(s) 89, 210, 446
HpySE526I ACGT 3 cut(s) 138, 243, 290
Hsp92II CATG 2 cut(s) 442, 541
HspAI GCGC 3 cut(s) 147, 270, 523
Kpn2I TCCGGA 1 cut(s) 385
KspI CCGCGG 1 cut(s) 276
Kzo9I GATC 2 cut(s) 423, 451
LguI GCTCTTC 2 cut(s) 302, 372
LmnI GCTCC 2 cut(s) 442, 547
LpnPI CCDG 8 cut(s) 76, 111, 233, 308, 399, 427, 446, 534
Lsp1109I GCAGC 1 cut(s) 350
LweI GCATC 1 cut(s) 158
MaeI CTAG 1 cut(s) 36
MaeII ACGT 3 cut(s) 138, 243, 290
MaeIII GTNAC 2 cut(s) 65, 428
MalI GATC 2 cut(s) 425, 453
MboI GATC 2 cut(s) 423, 451
MboII GAAGA 2 cut(s) 289, 389
MhlI GDGCHC 1 cut(s) 447
MluCI AATT 3 cut(s) 17, 162, 309
MmeI TCCRAC 2 cut(s) 78, 338
MnlI CCTC 8 cut(s) 104, 239, 263, 366, 443, 474, 505, 510
MroI TCCGGA 1 cut(s) 385
MroXI GAANNNNTTC 1 cut(s) 381
MspA1I CMGCKG 2 cut(s) 275, 341
MspI CCGG 4 cut(s) 63, 98, 386, 414
MspR9I CCNGG 2 cut(s) 98, 415
MvnI CGCG 2 cut(s) 275, 525
MwoI GCNNNNNNNGC 3 cut(s) 68, 272, 284
NciI CCSGG 2 cut(s) 98, 415
NcoI CCATGG 1 cut(s) 438
NdeII GATC 2 cut(s) 423, 451
NlaIII CATG 2 cut(s) 442, 541
NlaIV GGNNCC 3 cut(s) 193, 227, 543
NmuCI GTSAC 1 cut(s) 428
PciSI GCTCTTC 2 cut(s) 302, 372
PcsI WCGNNNNNNNCGW 2 cut(s) 150, 499
PdmI GAANNNNTTC 1 cut(s) 381
PfeI GAWTC 3 cut(s) 22, 39, 344
PfoI TCCNGGA 1 cut(s) 413
PkrI GCNGC 6 cut(s) 108, 274, 277, 340, 400, 527
Ppu21I YACGTR 1 cut(s) 291
Psp124BI GAGCTC 1 cut(s) 447
PspN4I GGNNCC 3 cut(s) 193, 227, 543
PvuII CAGCTG 1 cut(s) 341
RsaI GTAC 1 cut(s) 137
RsaNI GTAC 1 cut(s) 136
SacI GAGCTC 1 cut(s) 447
SacII CCGCGG 1 cut(s) 276
SapI GCTCTTC 2 cut(s) 302, 372
SatI GCNGC 6 cut(s) 107, 273, 276, 339, 399, 526
Sau3AI GATC 2 cut(s) 423, 451
ScrFI CCNGG 2 cut(s) 98, 415
SduI GDGCHC 1 cut(s) 447
SfaNI GCATC 1 cut(s) 158
Sfr303I CCGCGG 1 cut(s) 276
SgrBI CCGCGG 1 cut(s) 276
Sse9I AATT 3 cut(s) 17, 162, 309
SsiI CCGC 6 cut(s) 106, 273, 275, 278, 398, 525
SspMI CTAG 1 cut(s) 36
SstI GAGCTC 1 cut(s) 447
StyD4I CCNGG 2 cut(s) 96, 413
StyI CCWWGG 1 cut(s) 438
TaaI ACNGT 2 cut(s) 86, 135
TaiI ACGT 3 cut(s) 141, 246, 293
TasI AATT 3 cut(s) 17, 162, 309
TauI GCSGC 5 cut(s) 109, 275, 278, 401, 528
TfiI GAWTC 3 cut(s) 22, 39, 344
TscAI CASTG 3 cut(s) 227, 433, 454
TseFI GTSAC 1 cut(s) 428
TseI GCWGC 1 cut(s) 338
Tsp45I GTSAC 1 cut(s) 428
TspDTI ATGAA 1 cut(s) 156
TspGWI ACGGA 1 cut(s) 112
TspRI CASTG 3 cut(s) 227, 433, 454
XmnI GAANNNNTTC 1 cut(s) 381
XspI CTAG 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.