MD01G1018800.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007424
Physical Location & Seq
Forward (+)
8009755 .. 8011438
1684 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1018800.v1.1.491

Sequence Viewer

Length: 789 bp
ATGGCAATGTTCGTGGGTGGGAAATTTCAGCTGGCGAGTAACCCTAGGATTAACCAAAACGCCAACCACAGCCTGGAGGGCAACATAAACCCTCGCCGTCTGCCCCACCATCTCAGGCTGCCGCCTCGACGGCTCATCTCACTGTCCGTGCAGTCGCAGTATGTTAACGGAGCATCTGGGACGTCAGTTTCATCGCTTTACTCTGAAACCCTCGGATCTCCATCACTTTCTCAGACTTCACTTCTTCACCTCCCTCAGTGGAACCTCACCCACCGGCACATCGTCTTCCTCAATGTCGTTGCTTGCGCAGTAGCAGTTTCCGCGACGTGGCTCTTATGCTCTGCGATCCCCGCCCTACTGGCTTTCAAGAGGGCAGCTCAATCACTTGAAAAACTGATGGATGTTATTAGGGAAGAGCTTCCCGACACAATGGCTGCTGTTCGTTTATCCGGAATGGAGATTAGTGACCTCACTATGGAGCTCAGCGATCTTGGACAGGAAATTACACAAGGCGTTAGAAGTTCCACTCGAGCTGTTCGCGTGGCCGAGGAGAGGTTGCGCCGCTTGACAAACATGGCTCCATCAGTGGATGTAAAATTCATCTTTACTTGCTTAGCATCGGCGCAGGTAGTAAGTCAGAAAACTGAGGTGCCAGGGCCCGTTTTGGCTAAAACTGCAAGAGGCATACGGGAGGGGATCGTTAAGGGTCATGCTCTATGGCAAATGTTTTTCACTCTTACCCGGTTTTCTAGGTTGGCACTTAACTGTATAACAAATCGAACTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

263

Amino Acids

28.87

Weight (kDa)

10.32

Isoelectric Point (pI)

44.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 185
Acc16I TGCGCA 1 cut(s) 307
Acc36I ACCTGC 1 cut(s) 616
AccB1I GGYRCC 1 cut(s) 649
AccB7I CCANNNNNTGG 1 cut(s) 73
AccII CGCG 2 cut(s) 323, 540
AccIII TCCGGA 1 cut(s) 449
AciI CCGC 4 cut(s) 122, 321, 351, 562
AclWI GGATC 3 cut(s) 223, 340, 704
AcoI YGGCCR 1 cut(s) 543
AcsI RAATTY 2 cut(s) 23, 596
AcyI GRCGYC 1 cut(s) 182
AfiI CCNNNNNNNGG 4 cut(s) 73, 327, 475, 552
AgsI TTSAA 2 cut(s) 367, 389
AjiI CACGTC 1 cut(s) 327
AjnI CCWGG 2 cut(s) 72, 652
AluBI AGCT 5 cut(s) 31, 377, 418, 481, 533
AluI AGCT 5 cut(s) 31, 377, 418, 481, 533
Alw21I GWGCWC 1 cut(s) 483
AlwI GGATC 3 cut(s) 223, 340, 704
Ama87I CYCGRG 1 cut(s) 528
Aor13HI TCCGGA 1 cut(s) 449
AoxI GGCC 2 cut(s) 543, 656
ApaI GGGCCC 1 cut(s) 660
ApeKI GCWGC 3 cut(s) 118, 374, 434
ApoI RAATTY 2 cut(s) 23, 596
Asp700I GAANNNNTTC 1 cut(s) 417
AspA2I CCTAGG 1 cut(s) 44
AspLEI GCGC 3 cut(s) 308, 561, 625
AspS9I GGNCC 2 cut(s) 656, 657
AsuC2I CCSGG 1 cut(s) 742
AsuHPI GGTGA 2 cut(s) 239, 259
AvaI CYCGRG 1 cut(s) 528
AvrII CCTAGG 1 cut(s) 44
BaeGI GKGCMC 1 cut(s) 660
BanI GGYRCC 1 cut(s) 649
BanII GRGCYC 2 cut(s) 483, 660
BbsI GAAGAC 1 cut(s) 277
Bbv12I GWGCWC 1 cut(s) 483
BbvI GCAGC 3 cut(s) 105, 386, 421
BccI CCATC 4 cut(s) 117, 229, 391, 589
BceAI ACGGC 2 cut(s) 81, 146
BciT130I CCWGG 2 cut(s) 74, 654
BcnI CCSGG 1 cut(s) 742
BfaI CTAG 2 cut(s) 45, 750
BfuAI ACCTGC 1 cut(s) 616
BglI GCCNNNNNGGC 3 cut(s) 78, 130, 359
BisI GCNGC 5 cut(s) 119, 122, 375, 435, 562
BlnI CCTAGG 1 cut(s) 44
BlpI GCTNAGC 2 cut(s) 482, 613
BlsI GCNGC 5 cut(s) 120, 123, 376, 436, 563
Bme1390I CCNGG 3 cut(s) 74, 654, 742
BmeT110I CYCGRG 1 cut(s) 528
BmgBI CACGTC 1 cut(s) 327
BmgT120I GGNCC 2 cut(s) 656, 657
BmiI GGNNCC 4 cut(s) 263, 579, 651, 658
BmrFI CCNGG 3 cut(s) 74, 654, 742
BmsI GCATC 2 cut(s) 182, 626
BpiI GAAGAC 1 cut(s) 277
BplI GAGNNNNNCTC 2 cut(s) 361, 393
BpmI CTGGAG 1 cut(s) 95
Bpu1102I GCTNAGC 2 cut(s) 482, 613
BpuMI CCSGG 1 cut(s) 742
BsaBI GATNNNNATC 1 cut(s) 220
BsaHI GRCGYC 1 cut(s) 182
BsaJI CCNNGG 4 cut(s) 44, 211, 546, 653
BsaWI WCCGGW 1 cut(s) 449
Bsc4I CCNNNNNNNGG 4 cut(s) 73, 327, 475, 552
Bse118I RCCGGY 1 cut(s) 273
Bse1I ACTGG 1 cut(s) 363
Bse3DI GCAATG 1 cut(s) 12
Bse8I GATNNNNATC 1 cut(s) 220
BseAI TCCGGA 1 cut(s) 449
BseBI CCWGG 2 cut(s) 74, 654
BseDI CCNNGG 4 cut(s) 44, 211, 546, 653
BseGI GGATG 2 cut(s) 406, 595
BseJI GATNNNNATC 1 cut(s) 220
BseLI CCNNNNNNNGG 4 cut(s) 73, 327, 475, 552
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 5 cut(s) 127, 245, 269, 496, 636
BseNI ACTGG 1 cut(s) 363
BseRI GAGGAG 1 cut(s) 563
BseSI GKGCMC 1 cut(s) 660
BseXI GCAGC 3 cut(s) 105, 386, 421
BsgI GTGCAG 1 cut(s) 170
Bsh1236I CGCG 2 cut(s) 323, 540
BshFI GGCC 2 cut(s) 545, 658
BshNI GGYRCC 1 cut(s) 649
BsiHKAI GWGCWC 1 cut(s) 483
BsiHKCI CYCGRG 1 cut(s) 528
BsiSI CCGG 3 cut(s) 274, 450, 742
BslFI GGGAC 1 cut(s) 193
BslI CCNNNNNNNGG 4 cut(s) 73, 327, 475, 552
BsmFI GGGAC 1 cut(s) 193
BsnI GGCC 2 cut(s) 545, 658
BsoBI CYCGRG 1 cut(s) 528
Bsp120I GGGCCC 1 cut(s) 656
Bsp1286I GDGCHC 2 cut(s) 483, 660
Bsp13I TCCGGA 1 cut(s) 449
Bsp143I GATC 4 cut(s) 215, 345, 487, 696
Bsp1720I GCTNAGC 2 cut(s) 482, 613
BspACI CCGC 4 cut(s) 122, 321, 351, 562
BspANI GGCC 2 cut(s) 545, 658
BspCNI CTCAG 5 cut(s) 126, 244, 268, 495, 637
BspEI TCCGGA 1 cut(s) 449
BspFNI CGCG 2 cut(s) 323, 540
BspLI GGNNCC 4 cut(s) 263, 579, 651, 658
BspMI ACCTGC 1 cut(s) 616
BspPI GGATC 3 cut(s) 223, 340, 704
BspQI GCTCTTC 1 cut(s) 408
BspT107I GGYRCC 1 cut(s) 649
BsrDI GCAATG 1 cut(s) 12
BsrFI RCCGGY 1 cut(s) 273
BsrI ACTGG 1 cut(s) 363
BssAI RCCGGY 1 cut(s) 273
BssECI CCNNGG 4 cut(s) 44, 211, 546, 653
BssMI GATC 4 cut(s) 215, 345, 487, 696
BssNI GRCGYC 1 cut(s) 182
BssT1I CCWWGG 1 cut(s) 44
Bst2UI CCWGG 2 cut(s) 74, 654
Bst4CI ACNGT 2 cut(s) 144, 767
Bst6I CTCTTC 1 cut(s) 408
BstACI GRCGYC 1 cut(s) 182
BstC8I GCNNGC 2 cut(s) 33, 304
BstDEI CTNAG 7 cut(s) 113, 231, 255, 482, 613, 645, 783
BstF5I GGATG 2 cut(s) 406, 595
BstFNI CGCG 2 cut(s) 323, 540
BstHHI GCGC 3 cut(s) 308, 561, 625
BstKTI GATC 4 cut(s) 218, 348, 490, 699
BstMBI GATC 4 cut(s) 215, 345, 487, 696
BstMWI GCNNNNNNNGC 6 cut(s) 78, 130, 320, 350, 359, 674
BstNI CCWGG 2 cut(s) 74, 654
BstSCI CCNGG 3 cut(s) 72, 652, 740
BstSLI GKGCMC 1 cut(s) 660
BstUI CGCG 2 cut(s) 323, 540
BstV1I GCAGC 3 cut(s) 105, 386, 421
BstV2I GAAGAC 1 cut(s) 277
BstX2I RGATCY 1 cut(s) 215
BstYI RGATCY 1 cut(s) 215
BsuRI GGCC 2 cut(s) 545, 658
BtgZI GCGATG 1 cut(s) 177
BtrI CACGTC 1 cut(s) 327
BtsCI GGATG 2 cut(s) 406, 595
BtsIMutI CAGTG 3 cut(s) 140, 263, 591
BveI ACCTGC 1 cut(s) 616
Cac8I GCNNGC 2 cut(s) 33, 304
CfoI GCGC 3 cut(s) 308, 561, 625
Cfr10I RCCGGY 1 cut(s) 273
Cfr13I GGNCC 2 cut(s) 656, 657
CspCI CAANNNNNGTGG 1 cut(s) 29
CviAII CATG 2 cut(s) 574, 710
DdeI CTNAG 7 cut(s) 113, 231, 255, 482, 613, 645, 783
DpnI GATC 4 cut(s) 217, 347, 489, 698
DpnII GATC 4 cut(s) 215, 345, 487, 696
EaeI YGGCCR 1 cut(s) 543
Eam1104I CTCTTC 1 cut(s) 408
EarI CTCTTC 1 cut(s) 408
Ecl136II GAGCTC 1 cut(s) 481
Eco130I CCWWGG 1 cut(s) 44
Eco24I GRGCYC 2 cut(s) 483, 660
Eco53kI GAGCTC 1 cut(s) 481
Eco88I CYCGRG 1 cut(s) 528
EcoICRI GAGCTC 1 cut(s) 481
EcoO109I RGGNCCY 1 cut(s) 656
EcoRII CCWGG 2 cut(s) 72, 652
EcoT14I CCWWGG 1 cut(s) 44
EcoT38I GRGCYC 2 cut(s) 483, 660
ErhI CCWWGG 1 cut(s) 44
FaeI CATG 2 cut(s) 577, 713
FaiI YATR 9 cut(s) 86, 162, 337, 476, 575, 686, 711, 718, 770
FaqI GGGAC 1 cut(s) 193
FatI CATG 2 cut(s) 573, 709
FauI CCCGC 1 cut(s) 358
Fnu4HI GCNGC 5 cut(s) 119, 122, 375, 435, 562
FokI GGATG 2 cut(s) 413, 602
FriOI GRGCYC 2 cut(s) 483, 660
Fsp4HI GCNGC 5 cut(s) 119, 122, 375, 435, 562
FspBI CTAG 2 cut(s) 45, 750
FspI TGCGCA 1 cut(s) 307
GlaI GCGC 3 cut(s) 307, 560, 624
GluI GCNGC 5 cut(s) 119, 122, 375, 435, 562
GsuI CTGGAG 1 cut(s) 95
HaeIII GGCC 2 cut(s) 545, 658
HapII CCGG 3 cut(s) 274, 450, 742
HhaI GCGC 3 cut(s) 308, 561, 625
Hin1I GRCGYC 1 cut(s) 182
Hin1II CATG 2 cut(s) 577, 713
Hin6I GCGC 3 cut(s) 306, 559, 623
HinP1I GCGC 3 cut(s) 306, 559, 623
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HpaI GTTAAC 1 cut(s) 166
HpaII CCGG 3 cut(s) 274, 450, 742
HphI GGTGA 2 cut(s) 239, 259
Hpy166II GTNNAC 1 cut(s) 166
Hpy188I TCNGA 4 cut(s) 205, 215, 234, 639
Hpy188III TCNNGA 3 cut(s) 367, 422, 450
Hpy8I GTNNAC 1 cut(s) 166
Hpy99I CGWCG 2 cut(s) 132, 328
HpyCH4III ACNGT 2 cut(s) 144, 767
HpyCH4IV ACGT 2 cut(s) 182, 326
HpyCH4V TGCA 2 cut(s) 151, 677
HpyF10VI GCNNNNNNNGC 6 cut(s) 78, 130, 320, 350, 359, 674
HpyF3I CTNAG 7 cut(s) 113, 231, 255, 482, 613, 645, 783
HpySE526I ACGT 2 cut(s) 182, 326
Hsp92I GRCGYC 1 cut(s) 182
Hsp92II CATG 2 cut(s) 577, 713
HspAI GCGC 3 cut(s) 306, 559, 623
Kpn2I TCCGGA 1 cut(s) 449
KspAI GTTAAC 1 cut(s) 166
Kzo9I GATC 4 cut(s) 215, 345, 487, 696
LguI GCTCTTC 1 cut(s) 408
LmnI GCTCC 3 cut(s) 170, 478, 583
Lsp1109I GCAGC 3 cut(s) 105, 386, 421
LweI GCATC 2 cut(s) 182, 626
MaeI CTAG 2 cut(s) 45, 750
MaeII ACGT 2 cut(s) 182, 326
MaeIII GTNAC 2 cut(s) 38, 464
MalI GATC 4 cut(s) 217, 347, 489, 698
MboI GATC 4 cut(s) 215, 345, 487, 696
MboII GAAGA 3 cut(s) 236, 277, 425
MflI RGATCY 1 cut(s) 215
MhlI GDGCHC 2 cut(s) 483, 660
MluCI AATT 3 cut(s) 23, 501, 596
MroI TCCGGA 1 cut(s) 449
MroXI GAANNNNTTC 1 cut(s) 417
MseI TTAA 4 cut(s) 51, 165, 702, 762
MspA1I CMGCKG 1 cut(s) 31
MspI CCGG 3 cut(s) 274, 450, 742
MspR9I CCNGG 3 cut(s) 74, 654, 742
MvaI CCWGG 2 cut(s) 74, 654
MvnI CGCG 2 cut(s) 323, 540
MwoI GCNNNNNNNGC 6 cut(s) 78, 130, 320, 350, 359, 674
NciI CCSGG 1 cut(s) 742
NdeII GATC 4 cut(s) 215, 345, 487, 696
NlaIII CATG 2 cut(s) 577, 713
NlaIV GGNNCC 4 cut(s) 263, 579, 651, 658
NmeAIII GCCGAG 1 cut(s) 571
NmuCI GTSAC 1 cut(s) 464
NsbI TGCGCA 1 cut(s) 307
PaeR7I CTCGAG 1 cut(s) 528
PciSI GCTCTTC 1 cut(s) 408
PdmI GAANNNNTTC 1 cut(s) 417
PflMI CCANNNNNTGG 1 cut(s) 73
PkrI GCNGC 5 cut(s) 120, 123, 376, 436, 563
Psp124BI GAGCTC 1 cut(s) 483
Psp6I CCWGG 2 cut(s) 72, 652
PspGI CCWGG 2 cut(s) 72, 652
PspN4I GGNNCC 4 cut(s) 263, 579, 651, 658
PspOMI GGGCCC 1 cut(s) 656
PspPI GGNCC 2 cut(s) 656, 657
PspXI VCTCGAGB 1 cut(s) 528
PsuI RGATCY 1 cut(s) 215
PvuII CAGCTG 1 cut(s) 31
SacI GAGCTC 1 cut(s) 483
SapI GCTCTTC 1 cut(s) 408
SaqAI TTAA 4 cut(s) 51, 165, 702, 762
SatI GCNGC 5 cut(s) 119, 122, 375, 435, 562
Sau3AI GATC 4 cut(s) 215, 345, 487, 696
Sau96I GGNCC 2 cut(s) 656, 657
ScrFI CCNGG 3 cut(s) 74, 654, 742
SduI GDGCHC 2 cut(s) 483, 660
SfaNI GCATC 2 cut(s) 182, 626
Sfr274I CTCGAG 1 cut(s) 528
SlaI CTCGAG 1 cut(s) 528
SmlI CTYRAG 1 cut(s) 528
SmoI CTYRAG 1 cut(s) 528
Sse9I AATT 3 cut(s) 23, 501, 596
SsiI CCGC 4 cut(s) 122, 321, 351, 562
SspMI CTAG 2 cut(s) 45, 750
SstI GAGCTC 1 cut(s) 483
StyD4I CCNGG 3 cut(s) 72, 652, 740
StyI CCWWGG 1 cut(s) 44
TaaI ACNGT 2 cut(s) 144, 767
TaiI ACGT 2 cut(s) 185, 329
TaqI TCGA 3 cut(s) 127, 529, 778
TasI AATT 3 cut(s) 23, 501, 596
TauI GCSGC 2 cut(s) 124, 564
Tru1I TTAA 4 cut(s) 51, 165, 702, 762
Tru9I TTAA 4 cut(s) 51, 165, 702, 762
TscAI CASTG 3 cut(s) 147, 263, 591
TseFI GTSAC 1 cut(s) 464
TseI GCWGC 3 cut(s) 118, 374, 434
Tsp45I GTSAC 1 cut(s) 464
TspDTI ATGAA 2 cut(s) 180, 589
TspGWI ACGGA 2 cut(s) 136, 183
TspRI CASTG 3 cut(s) 147, 263, 591
Van91I CCANNNNNTGG 1 cut(s) 73
XapI RAATTY 2 cut(s) 23, 596
XcmI CCANNNNNNNNNTGG 1 cut(s) 70
XhoI CTCGAG 1 cut(s) 528
XmaJI CCTAGG 1 cut(s) 44
XmnI GAANNNNTTC 1 cut(s) 417
XspI CTAG 2 cut(s) 45, 750
ZraI GACGTC 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.