Rh2AG260700

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0007424
Physical Location & Seq
Forward (+)
30026136 .. 30027967
1832 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG260700.1

Sequence Viewer

Length: 591 bp
ATGTCGTTTGCTCTGAAATTGAATCTCTCAAACCCTAGAATCAAACCCAACTCCAACTTCGGAGGCCTCGCCGGTACCGCCACTAATCCCCACCGTCTCCCCTACCATCTCAGACTCCCCGCACGGCGGCTCAACTCCTTTTCCGTACAGTCAGAGTACGTTAACGGAGCTTCAGAGCCGTCAATTTCATCGCATTACACAGAAAGCATCGGAACCCCATCACCTTCGTCCCACCTTCTTCAGTGGAACCTCACCCAACGACACGTCGTCGTCCTCAATGTCGTTGCTTGCGCTGCGGCGGTTTCTGCAACGTGGCTCTTCTGCTCTGCAATTCCAACGCTTCTGGCTTTCAAGAGAGCAGCTGATTCACTTGCAAAGCTGATGGATGTTATGAGGGAAGAGCTTCCGGATACAATGGCGGCTGTTCGCTTATCCGGGATGGAGATCAGTGACCTAACCATGGAGCTCAGTGATCTTGGCCAGGAGATTACACAAGGTGTTAGGAGTTCTACTCGAGCTGTTCGTGTGGTGGAGGACAGGTTGCGCTGCTTGACAAACATGGCCCCTACAGGTTATCCATGTTTTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

196

Amino Acids

21.43

Weight (kDa)

8.8

Isoelectric Point (pI)

46.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 74
AccB1I GGYRCC 1 cut(s) 74
AccIII TCCGGA 1 cut(s) 406
AciI CCGC 6 cut(s) 78, 120, 127, 296, 299, 419
AcoI YGGCCR 1 cut(s) 478
AcuI CTGAAG 2 cut(s) 156, 224
AdeI CACNNNGTG 1 cut(s) 497
AfaI GTAC 3 cut(s) 76, 147, 158
AfiI CCNNNNNNNGG 2 cut(s) 126, 460
AflIII ACRYGT 1 cut(s) 262
AgsI TTSAA 2 cut(s) 22, 352
AhdI GACNNNNNGTC 1 cut(s) 266
AjiI CACGTC 1 cut(s) 265
AjnI CCWGG 1 cut(s) 480
AjuI GAANNNNNNNTTGG 2 cut(s) 41, 73
AluBI AGCT 6 cut(s) 170, 362, 379, 403, 466, 518
AluI AGCT 6 cut(s) 170, 362, 379, 403, 466, 518
Alw21I GWGCWC 1 cut(s) 468
Alw26I GTCTC 1 cut(s) 101
Ama87I CYCGRG 1 cut(s) 513
Aor13HI TCCGGA 1 cut(s) 406
AoxI GGCC 3 cut(s) 64, 478, 561
ApeKI GCWGC 3 cut(s) 293, 359, 546
Asp700I GAANNNNTTC 1 cut(s) 402
Asp718I GGTACC 1 cut(s) 74
AspLEI GCGC 2 cut(s) 293, 546
AspS9I GGNCC 1 cut(s) 562
AsuC2I CCSGG 1 cut(s) 436
AsuHPI GGTGA 2 cut(s) 213, 244
AvaI CYCGRG 1 cut(s) 513
BalI TGGCCA 1 cut(s) 480
BanI GGYRCC 1 cut(s) 74
BanII GRGCYC 1 cut(s) 468
Bbv12I GWGCWC 1 cut(s) 468
BbvI GCAGC 3 cut(s) 280, 371, 533
BccI CCATC 4 cut(s) 114, 226, 376, 433
BceAI ACGGC 2 cut(s) 140, 163
BciT130I CCWGG 1 cut(s) 482
BciVI GTATCC 1 cut(s) 403
BcnI CCSGG 1 cut(s) 436
BcoDI GTCTC 1 cut(s) 101
BfaI CTAG 1 cut(s) 36
BfmI CTRYAG 2 cut(s) 567, 587
BfuI GTATCC 1 cut(s) 403
BisI GCNGC 6 cut(s) 128, 294, 297, 360, 420, 547
BlsI GCNGC 6 cut(s) 129, 295, 298, 361, 421, 548
Bme1390I CCNGG 2 cut(s) 436, 482
BmeRI GACNNNNNGTC 1 cut(s) 266
BmeT110I CYCGRG 1 cut(s) 513
BmgBI CACGTC 1 cut(s) 265
BmgT120I GGNCC 1 cut(s) 562
BmiI GGNNCC 4 cut(s) 76, 214, 248, 564
BmrFI CCNGG 2 cut(s) 436, 482
BmsI GCATC 1 cut(s) 216
BplI GAGNNNNNCTC 2 cut(s) 496, 528
BpuMI CCSGG 1 cut(s) 436
BsaBI GATNNNNATC 1 cut(s) 443
BsaJI CCNNGG 1 cut(s) 459
BsaWI WCCGGW 1 cut(s) 406
BsaXI ACNNNNNCTCC 2 cut(s) 524, 554
Bsc4I CCNNNNNNNGG 2 cut(s) 126, 460
Bse118I RCCGGY 1 cut(s) 71
Bse8I GATNNNNATC 1 cut(s) 443
BseAI TCCGGA 1 cut(s) 406
BseBI CCWGG 1 cut(s) 482
BseDI CCNNGG 1 cut(s) 459
BseGI GGATG 2 cut(s) 391, 444
BseJI GATNNNNATC 1 cut(s) 443
BseLI CCNNNNNNNGG 2 cut(s) 126, 460
BseMII CTCAG 2 cut(s) 124, 481
BseXI GCAGC 3 cut(s) 280, 371, 533
BshFI GGCC 3 cut(s) 66, 480, 563
BshNI GGYRCC 1 cut(s) 74
BsiHKAI GWGCWC 1 cut(s) 468
BsiHKCI CYCGRG 1 cut(s) 513
BsiSI CCGG 3 cut(s) 72, 407, 435
BslFI GGGAC 1 cut(s) 214
BslI CCNNNNNNNGG 2 cut(s) 126, 460
BsmAI GTCTC 1 cut(s) 101
BsmBI CGTCTC 1 cut(s) 101
BsmFI GGGAC 1 cut(s) 214
BsnI GGCC 3 cut(s) 66, 480, 563
BsoBI CYCGRG 1 cut(s) 513
Bsp1286I GDGCHC 1 cut(s) 468
Bsp13I TCCGGA 1 cut(s) 406
Bsp143I GATC 2 cut(s) 444, 472
Bsp19I CCATGG 1 cut(s) 459
BspACI CCGC 6 cut(s) 78, 120, 127, 296, 299, 419
BspANI GGCC 3 cut(s) 66, 480, 563
BspCNI CTCAG 2 cut(s) 123, 480
BspEI TCCGGA 1 cut(s) 406
BspLI GGNNCC 4 cut(s) 76, 214, 248, 564
BspQI GCTCTTC 2 cut(s) 323, 393
BspT107I GGYRCC 1 cut(s) 74
BsrFI RCCGGY 1 cut(s) 71
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 1 cut(s) 459
BssMI GATC 2 cut(s) 444, 472
BssT1I CCWWGG 1 cut(s) 459
Bst2UI CCWGG 1 cut(s) 482
Bst4CI ACNGT 2 cut(s) 95, 150
Bst6I CTCTTC 2 cut(s) 323, 393
BstC8I GCNNGC 1 cut(s) 289
BstDEI CTNAG 2 cut(s) 110, 467
BstDSI CCRYGG 1 cut(s) 459
BstF5I GGATG 2 cut(s) 391, 444
BstHHI GCGC 2 cut(s) 293, 546
BstKTI GATC 2 cut(s) 447, 475
BstMAI GTCTC 1 cut(s) 101
BstMBI GATC 2 cut(s) 444, 472
BstMWI GCNNNNNNNGC 3 cut(s) 77, 293, 305
BstNI CCWGG 1 cut(s) 482
BstSCI CCNGG 2 cut(s) 434, 480
BstSFI CTRYAG 2 cut(s) 567, 587
BstV1I GCAGC 3 cut(s) 280, 371, 533
BsuI GTATCC 1 cut(s) 403
BsuRI GGCC 3 cut(s) 66, 480, 563
BtgI CCRYGG 1 cut(s) 459
BtgZI GCGATG 1 cut(s) 174
BtrI CACGTC 1 cut(s) 265
BtsCI GGATG 2 cut(s) 391, 444
BtsIMutI CAGTG 3 cut(s) 248, 454, 475
Cac8I GCNNGC 1 cut(s) 289
CfoI GCGC 2 cut(s) 293, 546
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 1 cut(s) 562
Csp6I GTAC 3 cut(s) 75, 146, 157
CviAII CATG 3 cut(s) 460, 559, 579
CviQI GTAC 3 cut(s) 75, 146, 157
DdeI CTNAG 2 cut(s) 110, 467
DpnI GATC 2 cut(s) 446, 474
DpnII GATC 2 cut(s) 444, 472
DraIII CACNNNGTG 1 cut(s) 497
DriI GACNNNNNGTC 1 cut(s) 266
EaeI YGGCCR 1 cut(s) 478
Eam1104I CTCTTC 2 cut(s) 323, 393
Eam1105I GACNNNNNGTC 1 cut(s) 266
EarI CTCTTC 2 cut(s) 323, 393
Ecl136II GAGCTC 1 cut(s) 466
Eco130I CCWWGG 1 cut(s) 459
Eco147I AGGCCT 1 cut(s) 66
Eco24I GRGCYC 1 cut(s) 468
Eco53kI GAGCTC 1 cut(s) 466
Eco57I CTGAAG 2 cut(s) 156, 224
Eco88I CYCGRG 1 cut(s) 513
EcoICRI GAGCTC 1 cut(s) 466
EcoRII CCWGG 1 cut(s) 480
EcoT14I CCWWGG 1 cut(s) 459
EcoT38I GRGCYC 1 cut(s) 468
ErhI CCWWGG 1 cut(s) 459
Esp3I CGTCTC 1 cut(s) 101
FaeI CATG 3 cut(s) 463, 562, 582
FaiI YATR 5 cut(s) 392, 461, 560, 580, 589
FaqI GGGAC 1 cut(s) 214
FatI CATG 3 cut(s) 459, 558, 578
FauI CCCGC 1 cut(s) 127
Fnu4HI GCNGC 6 cut(s) 128, 294, 297, 360, 420, 547
FokI GGATG 2 cut(s) 398, 451
FriOI GRGCYC 1 cut(s) 468
Fsp4HI GCNGC 6 cut(s) 128, 294, 297, 360, 420, 547
FspBI CTAG 1 cut(s) 36
GlaI GCGC 2 cut(s) 292, 545
GluI GCNGC 6 cut(s) 128, 294, 297, 360, 420, 547
HaeIII GGCC 3 cut(s) 66, 480, 563
HapII CCGG 3 cut(s) 72, 407, 435
HhaI GCGC 2 cut(s) 293, 546
Hin1II CATG 3 cut(s) 463, 562, 582
Hin6I GCGC 2 cut(s) 291, 544
HinP1I GCGC 2 cut(s) 291, 544
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 4 cut(s) 22, 39, 114, 365
HpaI GTTAAC 1 cut(s) 163
HpaII CCGG 3 cut(s) 72, 407, 435
HphI GGTGA 2 cut(s) 213, 244
Hpy166II GTNNAC 1 cut(s) 163
Hpy188I TCNGA 6 cut(s) 15, 62, 113, 154, 175, 212
Hpy188III TCNNGA 2 cut(s) 352, 407
Hpy8I GTNNAC 1 cut(s) 163
Hpy99I CGWCG 2 cut(s) 269, 272
HpyAV CCTTC 2 cut(s) 234, 245
HpyCH4III ACNGT 2 cut(s) 95, 150
HpyCH4IV ACGT 3 cut(s) 159, 264, 311
HpyCH4V TGCA 3 cut(s) 308, 329, 374
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 293, 305
HpyF3I CTNAG 2 cut(s) 110, 467
HpySE526I ACGT 3 cut(s) 159, 264, 311
Hsp92II CATG 3 cut(s) 463, 562, 582
HspAI GCGC 2 cut(s) 291, 544
Kpn2I TCCGGA 1 cut(s) 406
KpnI GGTACC 1 cut(s) 78
KspAI GTTAAC 1 cut(s) 163
Kzo9I GATC 2 cut(s) 444, 472
LguI GCTCTTC 2 cut(s) 323, 393
LmnI GCTCC 2 cut(s) 167, 463
LpnPI CCDG 8 cut(s) 85, 329, 420, 448, 467, 494, 523, 555
Lsp1109I GCAGC 3 cut(s) 280, 371, 533
LweI GCATC 1 cut(s) 216
MaeI CTAG 1 cut(s) 36
MaeII ACGT 3 cut(s) 159, 264, 311
MaeIII GTNAC 1 cut(s) 449
MalI GATC 2 cut(s) 446, 474
MboI GATC 2 cut(s) 444, 472
MboII GAAGA 3 cut(s) 230, 310, 410
MhlI GDGCHC 1 cut(s) 468
MlsI TGGCCA 1 cut(s) 480
MluCI AATT 3 cut(s) 17, 183, 330
MluNI TGGCCA 1 cut(s) 480
MlyI GAGTC 1 cut(s) 108
MmeI TCCRAC 2 cut(s) 78, 359
MnlI CCTC 6 cut(s) 56, 77, 260, 284, 387, 526
Mox20I TGGCCA 1 cut(s) 480
MroI TCCGGA 1 cut(s) 406
MroXI GAANNNNTTC 1 cut(s) 402
MscI TGGCCA 1 cut(s) 480
MseI TTAA 1 cut(s) 162
Msp20I TGGCCA 1 cut(s) 480
MspA1I CMGCKG 1 cut(s) 362
MspI CCGG 3 cut(s) 72, 407, 435
MspR9I CCNGG 2 cut(s) 436, 482
MvaI CCWGG 1 cut(s) 482
MwoI GCNNNNNNNGC 3 cut(s) 77, 293, 305
NciI CCSGG 1 cut(s) 436
NcoI CCATGG 1 cut(s) 459
NdeII GATC 2 cut(s) 444, 472
NlaIII CATG 3 cut(s) 463, 562, 582
NlaIV GGNNCC 4 cut(s) 76, 214, 248, 564
NmuCI GTSAC 1 cut(s) 449
PaeR7I CTCGAG 1 cut(s) 513
PceI AGGCCT 1 cut(s) 66
PciSI GCTCTTC 2 cut(s) 323, 393
PcsI WCGNNNNNNNCGW 1 cut(s) 520
PdmI GAANNNNTTC 1 cut(s) 402
PfeI GAWTC 3 cut(s) 22, 39, 365
PfoI TCCNGGA 1 cut(s) 434
PkrI GCNGC 6 cut(s) 129, 295, 298, 361, 421, 548
PleI GAGTC 1 cut(s) 108
PpsI GAGTC 1 cut(s) 108
Psp124BI GAGCTC 1 cut(s) 468
Psp6I CCWGG 1 cut(s) 480
PspGI CCWGG 1 cut(s) 480
PspN4I GGNNCC 4 cut(s) 76, 214, 248, 564
PspPI GGNCC 1 cut(s) 562
PspXI VCTCGAGB 1 cut(s) 513
PvuII CAGCTG 1 cut(s) 362
RsaI GTAC 3 cut(s) 76, 147, 158
RsaNI GTAC 3 cut(s) 75, 146, 157
SacI GAGCTC 1 cut(s) 468
SapI GCTCTTC 2 cut(s) 323, 393
SaqAI TTAA 1 cut(s) 162
SatI GCNGC 6 cut(s) 128, 294, 297, 360, 420, 547
Sau3AI GATC 2 cut(s) 444, 472
Sau96I GGNCC 1 cut(s) 562
SchI GAGTC 1 cut(s) 108
ScrFI CCNGG 2 cut(s) 436, 482
SduI GDGCHC 1 cut(s) 468
SfaNI GCATC 1 cut(s) 216
SfcI CTRYAG 2 cut(s) 567, 587
Sfr274I CTCGAG 1 cut(s) 513
SlaI CTCGAG 1 cut(s) 513
SmlI CTYRAG 1 cut(s) 513
SmoI CTYRAG 1 cut(s) 513
Sse9I AATT 3 cut(s) 17, 183, 330
SseBI AGGCCT 1 cut(s) 66
SsiI CCGC 6 cut(s) 78, 120, 127, 296, 299, 419
SspMI CTAG 1 cut(s) 36
SstI GAGCTC 1 cut(s) 468
StuI AGGCCT 1 cut(s) 66
StyD4I CCNGG 2 cut(s) 434, 480
StyI CCWWGG 1 cut(s) 459
TaaI ACNGT 2 cut(s) 95, 150
TaiI ACGT 3 cut(s) 162, 267, 314
TaqI TCGA 1 cut(s) 514
TasI AATT 3 cut(s) 17, 183, 330
TauI GCSGC 3 cut(s) 130, 299, 422
TfiI GAWTC 3 cut(s) 22, 39, 365
Tru1I TTAA 1 cut(s) 162
Tru9I TTAA 1 cut(s) 162
TscAI CASTG 3 cut(s) 248, 454, 475
TseFI GTSAC 1 cut(s) 449
TseI GCWGC 3 cut(s) 293, 359, 546
Tsp45I GTSAC 1 cut(s) 449
TspDTI ATGAA 1 cut(s) 177
TspGWI ACGGA 2 cut(s) 133, 180
TspRI CASTG 3 cut(s) 248, 454, 475
XhoI CTCGAG 1 cut(s) 513
XmnI GAANNNNTTC 1 cut(s) 402
XspI CTAG 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.