Rroxscaffold_2G00127400

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0007424
Physical Location & Seq
Reverse (-)
62840334 .. 62841504
1171 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00127400.1

Sequence Viewer

Length: 750 bp
ATGTCGTTTGCTCAGAAATTGAATCTCTCAAACCCTAGAATCAAACCCAACTCCAACTTCGGAGGCCTCGCCGGTACCGCCCTTAATCCCCACCGTCTCCCCTACCATCTAAGACTCCCGGCACGGCGGCTCAACTCCGTTTCCGTACAGTCAGAGTACGTTAACGGAGCTTCAGAGCCGTCAATTTCATCGCATTACACAGAGAGCATCGGAACCCCATCACCTTCGTCTCACCTTGTTCAGTGGAACCTCACCCAACGACATGTCGTCGTCCTCAATGTCGTTGCTTGCGCTGCGGCGGTTTCTGCAACGTGGCTCTTCTGCTCTGCAATTCCAACGCTTCTGGCTTTCAAGAGAGCAGCTGATTCACTTGCAAAGCTGATGGATGTTATGAGGGAAGAGCTTCCGGATACAATGGCGGCTGTTCGCTTATCCGGGATGGAGATCAGTGACCTAACCATGGAGCTCAGTGATCTTGGTCAGGAGATTACACAAGGTGTTAGGAGTTCTACTCGAGCTGTTCGTGTGGCCGAGGACAGGTTGCGCCGCTTGACAAACATGGCTCCTACAGCATCCGTGCAGGAAGTAGCAAGTCCGAAAAGTGAGGTGCCAGGGCCACCTTTGGCTACAACTGCAAGAAGCATACGAGAGGGAGTCGTGAAGGGTCGCGCACTCTGGCAAATGTTTTTCACCATCACCCGGTTTTCTAGGTTGGCACTCAACTTTTTGATCAGGCGGAAGAAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

249

Amino Acids

27.37

Weight (kDa)

10.5

Isoelectric Point (pI)

51.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 74
AccB1I GGYRCC 2 cut(s) 74, 607
AccII CGCG 1 cut(s) 669
AccIII TCCGGA 1 cut(s) 406
AciI CCGC 7 cut(s) 78, 127, 296, 299, 419, 547, 736
AcoI YGGCCR 1 cut(s) 528
AcuI CTGAAG 1 cut(s) 156
AdeI CACNNNGTG 1 cut(s) 497
AfaI GTAC 3 cut(s) 76, 147, 158
AfiI CCNNNNNNNGG 3 cut(s) 460, 537, 699
AflIII ACRYGT 1 cut(s) 262
AgsI TTSAA 2 cut(s) 22, 352
AhdI GACNNNNNGTC 1 cut(s) 266
AjnI CCWGG 1 cut(s) 610
AjuI GAANNNNNNNTTGG 2 cut(s) 41, 73
AluBI AGCT 6 cut(s) 170, 362, 379, 403, 466, 518
AluI AGCT 6 cut(s) 170, 362, 379, 403, 466, 518
Alw21I GWGCWC 1 cut(s) 468
Alw26I GTCTC 2 cut(s) 101, 234
Ama87I CYCGRG 1 cut(s) 513
Aor13HI TCCGGA 1 cut(s) 406
AoxI GGCC 3 cut(s) 64, 528, 614
ApeKI GCWGC 2 cut(s) 293, 359
Asp700I GAANNNNTTC 1 cut(s) 402
Asp718I GGTACC 1 cut(s) 74
AspLEI GCGC 3 cut(s) 293, 546, 671
AspS9I GGNCC 1 cut(s) 614
AsuC2I CCSGG 3 cut(s) 119, 436, 700
AsuHPI GGTGA 5 cut(s) 213, 224, 244, 682, 688
AvaI CYCGRG 1 cut(s) 513
BanI GGYRCC 2 cut(s) 74, 607
BanII GRGCYC 1 cut(s) 468
Bbv12I GWGCWC 1 cut(s) 468
BbvI GCAGC 2 cut(s) 280, 371
BccI CCATC 5 cut(s) 114, 226, 376, 433, 701
BceAI ACGGC 2 cut(s) 140, 163
BciT130I CCWGG 1 cut(s) 612
BciVI GTATCC 1 cut(s) 403
BclI TGATCA 1 cut(s) 729
BcnI CCSGG 3 cut(s) 119, 436, 700
BcoDI GTCTC 2 cut(s) 101, 234
BfaI CTAG 2 cut(s) 36, 708
BfmI CTRYAG 1 cut(s) 567
BfuI GTATCC 1 cut(s) 403
BisI GCNGC 6 cut(s) 128, 294, 297, 360, 420, 547
BlsI GCNGC 6 cut(s) 129, 295, 298, 361, 421, 548
Bme1390I CCNGG 4 cut(s) 119, 436, 612, 700
BmeRI GACNNNNNGTC 1 cut(s) 266
BmeT110I CYCGRG 1 cut(s) 513
BmgT120I GGNCC 1 cut(s) 614
BmiI GGNNCC 5 cut(s) 76, 214, 248, 564, 609
BmrFI CCNGG 4 cut(s) 119, 436, 612, 700
BmsI GCATC 2 cut(s) 216, 581
BplI GAGNNNNNCTC 2 cut(s) 496, 528
BpuMI CCSGG 3 cut(s) 119, 436, 700
BsaBI GATNNNNATC 1 cut(s) 443
BsaJI CCNNGG 3 cut(s) 459, 531, 611
BsaWI WCCGGW 1 cut(s) 406
Bsc4I CCNNNNNNNGG 3 cut(s) 460, 537, 699
Bse118I RCCGGY 1 cut(s) 71
Bse8I GATNNNNATC 1 cut(s) 443
BseAI TCCGGA 1 cut(s) 406
BseBI CCWGG 1 cut(s) 612
BseDI CCNNGG 3 cut(s) 459, 531, 611
BseGI GGATG 3 cut(s) 391, 444, 572
BseJI GATNNNNATC 1 cut(s) 443
BseLI CCNNNNNNNGG 3 cut(s) 460, 537, 699
BseMII CTCAG 2 cut(s) 26, 481
BseXI GCAGC 2 cut(s) 280, 371
BsgI GTGCAG 1 cut(s) 599
Bsh1236I CGCG 1 cut(s) 669
BshFI GGCC 3 cut(s) 66, 530, 616
BshNI GGYRCC 2 cut(s) 74, 607
BsiHKAI GWGCWC 1 cut(s) 468
BsiHKCI CYCGRG 1 cut(s) 513
BsiSI CCGG 5 cut(s) 72, 119, 407, 435, 700
BslI CCNNNNNNNGG 3 cut(s) 460, 537, 699
BsmAI GTCTC 2 cut(s) 101, 234
BsmBI CGTCTC 2 cut(s) 101, 234
BsnI GGCC 3 cut(s) 66, 530, 616
BsoBI CYCGRG 1 cut(s) 513
Bsp1286I GDGCHC 1 cut(s) 468
Bsp13I TCCGGA 1 cut(s) 406
Bsp143I GATC 3 cut(s) 444, 472, 729
Bsp19I CCATGG 1 cut(s) 459
BspACI CCGC 7 cut(s) 78, 127, 296, 299, 419, 547, 736
BspANI GGCC 3 cut(s) 66, 530, 616
BspCNI CTCAG 2 cut(s) 25, 480
BspEI TCCGGA 1 cut(s) 406
BspFNI CGCG 1 cut(s) 669
BspLI GGNNCC 5 cut(s) 76, 214, 248, 564, 609
BspQI GCTCTTC 2 cut(s) 323, 393
BspT107I GGYRCC 2 cut(s) 74, 607
BsrFI RCCGGY 1 cut(s) 71
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 3 cut(s) 459, 531, 611
BssMI GATC 3 cut(s) 444, 472, 729
BssT1I CCWWGG 1 cut(s) 459
Bst2UI CCWGG 1 cut(s) 612
Bst4CI ACNGT 2 cut(s) 95, 150
Bst6I CTCTTC 2 cut(s) 323, 393
BstC8I GCNNGC 1 cut(s) 289
BstDEI CTNAG 3 cut(s) 12, 110, 467
BstDSI CCRYGG 1 cut(s) 459
BstF5I GGATG 3 cut(s) 391, 444, 572
BstFNI CGCG 1 cut(s) 669
BstHHI GCGC 3 cut(s) 293, 546, 671
BstKTI GATC 3 cut(s) 447, 475, 732
BstMAI GTCTC 2 cut(s) 101, 234
BstMBI GATC 3 cut(s) 444, 472, 729
BstMWI GCNNNNNNNGC 6 cut(s) 77, 293, 305, 569, 632, 742
BstNI CCWGG 1 cut(s) 612
BstNSI RCATGY 1 cut(s) 266
BstSCI CCNGG 4 cut(s) 117, 434, 610, 698
BstSFI CTRYAG 1 cut(s) 567
BstUI CGCG 1 cut(s) 669
BstV1I GCAGC 2 cut(s) 280, 371
BsuI GTATCC 1 cut(s) 403
BsuRI GGCC 3 cut(s) 66, 530, 616
BtgI CCRYGG 1 cut(s) 459
BtgZI GCGATG 1 cut(s) 174
BtsCI GGATG 3 cut(s) 391, 444, 572
BtsIMutI CAGTG 3 cut(s) 248, 454, 475
Cac8I GCNNGC 1 cut(s) 289
CfoI GCGC 3 cut(s) 293, 546, 671
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 1 cut(s) 614
Csp6I GTAC 3 cut(s) 75, 146, 157
CviAII CATG 3 cut(s) 263, 460, 559
CviQI GTAC 3 cut(s) 75, 146, 157
DdeI CTNAG 3 cut(s) 12, 110, 467
DpnI GATC 3 cut(s) 446, 474, 731
DpnII GATC 3 cut(s) 444, 472, 729
DraIII CACNNNGTG 1 cut(s) 497
DriI GACNNNNNGTC 1 cut(s) 266
EaeI YGGCCR 1 cut(s) 528
Eam1104I CTCTTC 2 cut(s) 323, 393
Eam1105I GACNNNNNGTC 1 cut(s) 266
EarI CTCTTC 2 cut(s) 323, 393
Ecl136II GAGCTC 1 cut(s) 466
Eco130I CCWWGG 1 cut(s) 459
Eco147I AGGCCT 1 cut(s) 66
Eco24I GRGCYC 1 cut(s) 468
Eco53kI GAGCTC 1 cut(s) 466
Eco57I CTGAAG 1 cut(s) 156
Eco88I CYCGRG 1 cut(s) 513
EcoICRI GAGCTC 1 cut(s) 466
EcoRII CCWGG 1 cut(s) 610
EcoT14I CCWWGG 1 cut(s) 459
EcoT38I GRGCYC 1 cut(s) 468
ErhI CCWWGG 1 cut(s) 459
Esp3I CGTCTC 2 cut(s) 101, 234
FaeI CATG 3 cut(s) 266, 463, 562
FaiI YATR 5 cut(s) 264, 392, 461, 560, 644
FatI CATG 3 cut(s) 262, 459, 558
FbaI TGATCA 1 cut(s) 729
Fnu4HI GCNGC 6 cut(s) 128, 294, 297, 360, 420, 547
FokI GGATG 3 cut(s) 398, 451, 559
FriOI GRGCYC 1 cut(s) 468
Fsp4HI GCNGC 6 cut(s) 128, 294, 297, 360, 420, 547
FspBI CTAG 2 cut(s) 36, 708
GlaI GCGC 3 cut(s) 292, 545, 670
GluI GCNGC 6 cut(s) 128, 294, 297, 360, 420, 547
HaeIII GGCC 3 cut(s) 66, 530, 616
HapII CCGG 5 cut(s) 72, 119, 407, 435, 700
HhaI GCGC 3 cut(s) 293, 546, 671
Hin1II CATG 3 cut(s) 266, 463, 562
Hin6I GCGC 3 cut(s) 291, 544, 669
HinP1I GCGC 3 cut(s) 291, 544, 669
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 5 cut(s) 22, 39, 114, 365, 654
HpaI GTTAAC 1 cut(s) 163
HpaII CCGG 5 cut(s) 72, 119, 407, 435, 700
HphI GGTGA 5 cut(s) 213, 224, 244, 682, 688
Hpy166II GTNNAC 1 cut(s) 163
Hpy188I TCNGA 6 cut(s) 15, 62, 154, 175, 212, 597
Hpy188III TCNNGA 4 cut(s) 352, 407, 482, 658
Hpy8I GTNNAC 1 cut(s) 163
Hpy99I CGWCG 1 cut(s) 272
HpyAV CCTTC 2 cut(s) 234, 655
HpyCH4III ACNGT 2 cut(s) 95, 150
HpyCH4IV ACGT 2 cut(s) 159, 311
HpyCH4V TGCA 5 cut(s) 308, 329, 374, 580, 635
HpyF10VI GCNNNNNNNGC 6 cut(s) 77, 293, 305, 569, 632, 742
HpyF3I CTNAG 3 cut(s) 12, 110, 467
HpySE526I ACGT 2 cut(s) 159, 311
Hsp92II CATG 3 cut(s) 266, 463, 562
HspAI GCGC 3 cut(s) 291, 544, 669
Kpn2I TCCGGA 1 cut(s) 406
KpnI GGTACC 1 cut(s) 78
Ksp22I TGATCA 1 cut(s) 729
KspAI GTTAAC 1 cut(s) 163
Kzo9I GATC 3 cut(s) 444, 472, 729
LguI GCTCTTC 2 cut(s) 323, 393
LmnI GCTCC 3 cut(s) 167, 463, 568
Lsp1109I GCAGC 2 cut(s) 280, 371
LweI GCATC 2 cut(s) 216, 581
MaeI CTAG 2 cut(s) 36, 708
MaeII ACGT 2 cut(s) 159, 311
MaeIII GTNAC 1 cut(s) 449
MalI GATC 3 cut(s) 446, 474, 731
MboI GATC 3 cut(s) 444, 472, 729
MboII GAAGA 2 cut(s) 310, 410
MhlI GDGCHC 1 cut(s) 468
MluCI AATT 3 cut(s) 17, 183, 330
MlyI GAGTC 2 cut(s) 108, 663
MmeI TCCRAC 2 cut(s) 78, 359
MnlI CCTC 8 cut(s) 56, 77, 260, 284, 387, 526, 598, 643
MroI TCCGGA 1 cut(s) 406
MroXI GAANNNNTTC 1 cut(s) 402
MseI TTAA 2 cut(s) 84, 162
MspA1I CMGCKG 1 cut(s) 362
MspI CCGG 5 cut(s) 72, 119, 407, 435, 700
MspR9I CCNGG 4 cut(s) 119, 436, 612, 700
MvaI CCWGG 1 cut(s) 612
MvnI CGCG 1 cut(s) 669
MwoI GCNNNNNNNGC 6 cut(s) 77, 293, 305, 569, 632, 742
NciI CCSGG 3 cut(s) 119, 436, 700
NcoI CCATGG 1 cut(s) 459
NdeII GATC 3 cut(s) 444, 472, 729
NlaIII CATG 3 cut(s) 266, 463, 562
NlaIV GGNNCC 5 cut(s) 76, 214, 248, 564, 609
NmeAIII GCCGAG 1 cut(s) 556
NmuCI GTSAC 1 cut(s) 449
NspI RCATGY 1 cut(s) 266
PaeR7I CTCGAG 1 cut(s) 513
PceI AGGCCT 1 cut(s) 66
PciI ACATGT 1 cut(s) 262
PciSI GCTCTTC 2 cut(s) 323, 393
PcsI WCGNNNNNNNCGW 1 cut(s) 520
PdmI GAANNNNTTC 1 cut(s) 402
PfeI GAWTC 3 cut(s) 22, 39, 365
PfoI TCCNGGA 1 cut(s) 434
PkrI GCNGC 6 cut(s) 129, 295, 298, 361, 421, 548
PleI GAGTC 2 cut(s) 108, 662
PpsI GAGTC 2 cut(s) 108, 662
PscI ACATGT 1 cut(s) 262
Psp124BI GAGCTC 1 cut(s) 468
Psp6I CCWGG 1 cut(s) 610
PspGI CCWGG 1 cut(s) 610
PspN4I GGNNCC 5 cut(s) 76, 214, 248, 564, 609
PspPI GGNCC 1 cut(s) 614
PspXI VCTCGAGB 1 cut(s) 513
PvuII CAGCTG 1 cut(s) 362
RsaI GTAC 3 cut(s) 76, 147, 158
RsaNI GTAC 3 cut(s) 75, 146, 157
SacI GAGCTC 1 cut(s) 468
SapI GCTCTTC 2 cut(s) 323, 393
SaqAI TTAA 2 cut(s) 84, 162
SatI GCNGC 6 cut(s) 128, 294, 297, 360, 420, 547
Sau3AI GATC 3 cut(s) 444, 472, 729
Sau96I GGNCC 1 cut(s) 614
SchI GAGTC 2 cut(s) 108, 663
ScrFI CCNGG 4 cut(s) 119, 436, 612, 700
SduI GDGCHC 1 cut(s) 468
SfaNI GCATC 2 cut(s) 216, 581
SfcI CTRYAG 1 cut(s) 567
Sfr274I CTCGAG 1 cut(s) 513
SlaI CTCGAG 1 cut(s) 513
SmlI CTYRAG 1 cut(s) 513
SmoI CTYRAG 1 cut(s) 513
Sse9I AATT 3 cut(s) 17, 183, 330
SseBI AGGCCT 1 cut(s) 66
SsiI CCGC 7 cut(s) 78, 127, 296, 299, 419, 547, 736
SspMI CTAG 2 cut(s) 36, 708
SstI GAGCTC 1 cut(s) 468
StuI AGGCCT 1 cut(s) 66
StyD4I CCNGG 4 cut(s) 117, 434, 610, 698
StyI CCWWGG 1 cut(s) 459
TaaI ACNGT 2 cut(s) 95, 150
TaiI ACGT 2 cut(s) 162, 314
TaqI TCGA 1 cut(s) 514
TasI AATT 3 cut(s) 17, 183, 330
TauI GCSGC 4 cut(s) 130, 299, 422, 549
TfiI GAWTC 3 cut(s) 22, 39, 365
Tru1I TTAA 2 cut(s) 84, 162
Tru9I TTAA 2 cut(s) 84, 162
TscAI CASTG 3 cut(s) 248, 454, 475
TseFI GTSAC 1 cut(s) 449
TseI GCWGC 2 cut(s) 293, 359
Tsp45I GTSAC 1 cut(s) 449
TspDTI ATGAA 1 cut(s) 177
TspGWI ACGGA 4 cut(s) 127, 133, 180, 565
TspRI CASTG 3 cut(s) 248, 454, 475
XceI RCATGY 1 cut(s) 266
XhoI CTCGAG 1 cut(s) 513
XmnI GAANNNNTTC 1 cut(s) 402
XspI CTAG 2 cut(s) 36, 708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.