FvH4_1g24901

Plant self-incompatibility protein S1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0012568
Physical Location & Seq
Forward (+)
16686422 .. 16687187
766 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g24901.t1

Sequence Viewer

Length: 462 bp
ATGGGTGGCACACTAGTTCTTTCCACGCTAGTCATTTCAGTTGTACTACTACTGAACCAATCCTCAGTATTTGTTTCAGGATTGATCCCCGGAGAGGTTACTGTTCATATGGCCAATAACTTGCTCCCAACTGAACAGGAACTCACTGTTCACTGCCAATCCGGAGACGACGATCTGGGAATTCAAAACCTTCCTTACCTAGCCGATTACCAGTTCAAATTTCATATCAACGTCGGAGACACGACCCTCTTCTTTTGTAACTTTGAGTGGCTAAACACTACGACGTCAGATTACCAAACAGTTTCCGGCAAGTATGACATATACGTGGCCAAGAGGGATGAGTACCGGTGCGGCACCTACTGCAACTGGAGAATTCAAGAAGAAGGCCTCTTTTCATACAACGACAACCCGGATGCCAAATACTGGGAGCGCCTCTACACATGGCCTGGCCACACCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

154

Amino Acids

17.51

Weight (kDa)

4.54

Isoelectric Point (pI)

19.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 34 - 148 2.1e-26 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 287
AccB1I GGYRCC 1 cut(s) 353
AccB7I CCANNNNNTGG 1 cut(s) 423
AccIII TCCGGA 1 cut(s) 161
AciI CCGC 1 cut(s) 351
AclWI GGATC 1 cut(s) 79
AcoI YGGCCR 3 cut(s) 111, 327, 448
AcsI RAATTY 3 cut(s) 180, 218, 372
AcyI GRCGYC 1 cut(s) 284
AfaI GTAC 2 cut(s) 45, 344
AfiI CCNNNNNNNGG 2 cut(s) 94, 423
AgeI ACCGGT 1 cut(s) 345
AgsI TTSAA 3 cut(s) 185, 217, 377
AhlI ACTAGT 1 cut(s) 13
AjnI CCWGG 1 cut(s) 445
Alw26I GTCTC 2 cut(s) 159, 231
AlwI GGATC 1 cut(s) 79
Aor13HI TCCGGA 1 cut(s) 161
AoxI GGCC 5 cut(s) 111, 327, 385, 443, 448
ApoI RAATTY 3 cut(s) 180, 218, 372
AsiGI ACCGGT 1 cut(s) 345
AspLEI GCGC 1 cut(s) 432
AsuC2I CCSGG 2 cut(s) 90, 410
BalI TGGCCA 3 cut(s) 113, 329, 450
BanI GGYRCC 1 cut(s) 353
BciT130I CCWGG 1 cut(s) 447
BcnI CCSGG 2 cut(s) 90, 410
BcoDI GTCTC 2 cut(s) 159, 231
BcuI ACTAGT 1 cut(s) 13
BfaI CTAG 3 cut(s) 14, 29, 200
BfoI RGCGCY 1 cut(s) 433
BisI GCNGC 1 cut(s) 352
BlsI GCNGC 1 cut(s) 353
Bme1390I CCNGG 3 cut(s) 90, 410, 447
BmiI GGNNCC 1 cut(s) 355
BmrFI CCNGG 3 cut(s) 90, 410, 447
BmrI ACTGGG 1 cut(s) 433
BmsI GCATC 1 cut(s) 403
BmuI ACTGGG 1 cut(s) 433
BpmI CTGGAG 1 cut(s) 388
BpuMI CCSGG 2 cut(s) 90, 410
BsaAI YACGTR 1 cut(s) 325
BsaHI GRCGYC 1 cut(s) 284
BsaJI CCNNGG 1 cut(s) 88
BsaWI WCCGGW 2 cut(s) 161, 345
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 423
Bse118I RCCGGY 1 cut(s) 345
Bse1I ACTGG 3 cut(s) 211, 371, 428
BseAI TCCGGA 1 cut(s) 161
BseBI CCWGG 1 cut(s) 447
BseDI CCNNGG 1 cut(s) 88
BseGI GGATG 2 cut(s) 343, 418
BseLI CCNNNNNNNGG 2 cut(s) 94, 423
BseMII CTCAG 1 cut(s) 78
BseNI ACTGG 3 cut(s) 211, 371, 428
BshFI GGCC 5 cut(s) 113, 329, 387, 445, 450
BshNI GGYRCC 1 cut(s) 353
BshTI ACCGGT 1 cut(s) 345
BsiSI CCGG 5 cut(s) 90, 162, 306, 346, 410
BslI CCNNNNNNNGG 2 cut(s) 94, 423
BsmAI GTCTC 2 cut(s) 159, 231
BsmBI CGTCTC 1 cut(s) 159
BsnI GGCC 5 cut(s) 113, 329, 387, 445, 450
Bsp13I TCCGGA 1 cut(s) 161
Bsp143I GATC 2 cut(s) 84, 172
BspACI CCGC 1 cut(s) 351
BspANI GGCC 5 cut(s) 113, 329, 387, 445, 450
BspCNI CTCAG 1 cut(s) 77
BspEI TCCGGA 1 cut(s) 161
BspLI GGNNCC 1 cut(s) 355
BspPI GGATC 1 cut(s) 79
BspT107I GGYRCC 1 cut(s) 353
BsrFI RCCGGY 1 cut(s) 345
BsrI ACTGG 3 cut(s) 211, 371, 428
BssAI RCCGGY 1 cut(s) 345
BssECI CCNNGG 1 cut(s) 88
BssMI GATC 2 cut(s) 84, 172
BssNI GRCGYC 1 cut(s) 284
Bst2UI CCWGG 1 cut(s) 447
Bst4CI ACNGT 3 cut(s) 103, 148, 301
Bst6I CTCTTC 1 cut(s) 254
BstACI GRCGYC 1 cut(s) 284
BstAPI GCANNNNNTGC 1 cut(s) 360
BstBAI YACGTR 1 cut(s) 325
BstDEI CTNAG 1 cut(s) 64
BstF5I GGATG 2 cut(s) 343, 418
BstH2I RGCGCY 1 cut(s) 433
BstHHI GCGC 1 cut(s) 432
BstKTI GATC 2 cut(s) 87, 175
BstMAI GTCTC 2 cut(s) 159, 231
BstMBI GATC 2 cut(s) 84, 172
BstMWI GCNNNNNNNGC 1 cut(s) 360
BstNI CCWGG 1 cut(s) 447
BstSCI CCNGG 3 cut(s) 88, 408, 445
BsuRI GGCC 5 cut(s) 113, 329, 387, 445, 450
BtsCI GGATG 2 cut(s) 343, 418
BtsI GCAGTG 1 cut(s) 151
BtsIMutI CAGTG 2 cut(s) 144, 151
CfoI GCGC 1 cut(s) 432
Cfr10I RCCGGY 1 cut(s) 345
Csp6I GTAC 2 cut(s) 44, 343
CspAI ACCGGT 1 cut(s) 345
CspCI CAANNNNNGTGG 1 cut(s) 440
CviAII CATG 1 cut(s) 441
CviJI RGCY 7 cut(s) 113, 203, 271, 329, 387, 445, 450
CviKI_1 RGCY 7 cut(s) 113, 203, 271, 329, 387, 445, 450
CviQI GTAC 2 cut(s) 44, 343
DdeI CTNAG 1 cut(s) 64
DpnI GATC 2 cut(s) 86, 174
DpnII GATC 2 cut(s) 84, 172
EaeI YGGCCR 3 cut(s) 111, 327, 448
Eam1104I CTCTTC 1 cut(s) 254
EarI CTCTTC 1 cut(s) 254
Eco147I AGGCCT 1 cut(s) 387
EcoRI GAATTC 2 cut(s) 180, 372
EcoRII CCWGG 1 cut(s) 445
Esp3I CGTCTC 1 cut(s) 159
FaeI CATG 1 cut(s) 444
FaiI YATR 8 cut(s) 108, 110, 225, 315, 320, 322, 397, 442
FatI CATG 1 cut(s) 440
FauNDI CATATG 1 cut(s) 108
Fnu4HI GCNGC 1 cut(s) 352
FokI GGATG 2 cut(s) 350, 425
Fsp4HI GCNGC 1 cut(s) 352
FspBI CTAG 3 cut(s) 14, 29, 200
GlaI GCGC 1 cut(s) 431
GluI GCNGC 1 cut(s) 352
GsuI CTGGAG 1 cut(s) 388
HaeII RGCGCY 1 cut(s) 433
HaeIII GGCC 5 cut(s) 113, 329, 387, 445, 450
HapII CCGG 5 cut(s) 90, 162, 306, 346, 410
HhaI GCGC 1 cut(s) 432
Hin1I GRCGYC 1 cut(s) 284
Hin1II CATG 1 cut(s) 444
Hin6I GCGC 1 cut(s) 430
HinP1I GCGC 1 cut(s) 430
HpaII CCGG 5 cut(s) 90, 162, 306, 346, 410
Hpy166II GTNNAC 1 cut(s) 151
Hpy188I TCNGA 2 cut(s) 236, 289
Hpy188III TCNNGA 3 cut(s) 78, 162, 377
Hpy8I GTNNAC 1 cut(s) 151
Hpy99I CGWCG 3 cut(s) 173, 236, 286
HpyAV CCTTC 2 cut(s) 200, 377
HpyCH4III ACNGT 3 cut(s) 103, 148, 301
HpyCH4IV ACGT 3 cut(s) 231, 284, 324
HpyCH4V TGCA 1 cut(s) 363
HpyF10VI GCNNNNNNNGC 1 cut(s) 360
HpyF3I CTNAG 1 cut(s) 64
HpySE526I ACGT 3 cut(s) 231, 284, 324
Hsp92I GRCGYC 1 cut(s) 284
Hsp92II CATG 1 cut(s) 444
HspAI GCGC 1 cut(s) 430
Kpn2I TCCGGA 1 cut(s) 161
Kzo9I GATC 2 cut(s) 84, 172
LmnI GCTCC 2 cut(s) 129, 427
LweI GCATC 1 cut(s) 403
MaeI CTAG 3 cut(s) 14, 29, 200
MaeII ACGT 3 cut(s) 231, 284, 324
MaeIII GTNAC 2 cut(s) 97, 257
MalI GATC 2 cut(s) 86, 174
MboI GATC 2 cut(s) 84, 172
MboII GAAGA 2 cut(s) 241, 392
MlsI TGGCCA 3 cut(s) 113, 329, 450
MluCI AATT 3 cut(s) 180, 218, 372
MluNI TGGCCA 3 cut(s) 113, 329, 450
MmeI TCCRAC 1 cut(s) 214
MnlI CCTC 6 cut(s) 73, 88, 257, 327, 398, 443
Mox20I TGGCCA 3 cut(s) 113, 329, 450
MroI TCCGGA 1 cut(s) 161
MscI TGGCCA 3 cut(s) 113, 329, 450
MslI CAYNNNNRTG 1 cut(s) 323
Msp20I TGGCCA 3 cut(s) 113, 329, 450
MspI CCGG 5 cut(s) 90, 162, 306, 346, 410
MspR9I CCNGG 3 cut(s) 90, 410, 447
MvaI CCWGG 1 cut(s) 447
MwoI GCNNNNNNNGC 1 cut(s) 360
NciI CCSGG 2 cut(s) 90, 410
NdeI CATATG 1 cut(s) 108
NdeII GATC 2 cut(s) 84, 172
NlaIII CATG 1 cut(s) 444
NlaIV GGNNCC 1 cut(s) 355
PceI AGGCCT 1 cut(s) 387
PflMI CCANNNNNTGG 1 cut(s) 423
PinAI ACCGGT 1 cut(s) 345
PkrI GCNGC 1 cut(s) 353
Ppu21I YACGTR 1 cut(s) 325
Psp6I CCWGG 1 cut(s) 445
PspGI CCWGG 1 cut(s) 445
PspN4I GGNNCC 1 cut(s) 355
RsaI GTAC 2 cut(s) 45, 344
RsaNI GTAC 2 cut(s) 44, 343
RseI CAYNNNNRTG 1 cut(s) 323
SatI GCNGC 1 cut(s) 352
Sau3AI GATC 2 cut(s) 84, 172
ScrFI CCNGG 3 cut(s) 90, 410, 447
SetI ASST 7 cut(s) 99, 192, 201, 234, 287, 327, 359
SfaNI GCATC 1 cut(s) 403
SmiMI CAYNNNNRTG 1 cut(s) 323
SpeI ACTAGT 1 cut(s) 13
Sse9I AATT 3 cut(s) 180, 218, 372
SseBI AGGCCT 1 cut(s) 387
SsiI CCGC 1 cut(s) 351
SspMI CTAG 3 cut(s) 14, 29, 200
StuI AGGCCT 1 cut(s) 387
StyD4I CCNGG 3 cut(s) 88, 408, 445
TaaI ACNGT 3 cut(s) 103, 148, 301
TaiI ACGT 3 cut(s) 234, 287, 327
TasI AATT 3 cut(s) 180, 218, 372
TatI WGTACW 1 cut(s) 43
TauI GCSGC 1 cut(s) 354
TscAI CASTG 2 cut(s) 151, 158
TspDTI ATGAA 3 cut(s) 95, 212, 384
TspRI CASTG 2 cut(s) 151, 158
Van91I CCANNNNNTGG 1 cut(s) 423
XapI RAATTY 3 cut(s) 180, 218, 372
XspI CTAG 3 cut(s) 14, 29, 200
ZraI GACGTC 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.