MD06G1007700.v1.1

Plant self-incompatibility protein S1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0012568
Physical Location & Seq
Reverse (-)
856064 .. 856576
513 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1007700.v1.1.491

Sequence Viewer

Length: 513 bp
ATGGCTGCAAGGACCATTTTCATTAGTGTTGCCGTGCTTGCCCTAGGCCAATTTGTTTCATCAACTTTTTTCGGAAAAATCCATATTGACGTAGAGAACACCCTAGTGTCAAACACTGTTGTTGAACTTCATTGCATGTCCAAGAGTGATGACTTGGGCAACCATGTAATTCCACACAGCGGCGCGTACAACTTTACGTTCCACGATGATTTCTGGGACAGAACCCTCTTCTTTTGCTACATGCAGTGGCGAAGGTCTGATGGGAAATGGGTAAAGGCCGCTTTTAACATATACAAGCCAAAGAGGGATCAAAAACGTTGCATGCATCGTTGCAAGTGGCAGATAAATGAATACGGCATCTTTTCCTTCACCCTTGATACGAAGGTGTGGACAAATTTCTACAAGAACTTCACTATTGAGGATAGGGCGAGTACACCAAATAATGCTCCGCCCAGTGTTTACTCTTCACTCGGCTTTATTACTCTCGTATTGCACTTTTTATTCATTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

171

Amino Acids

19.83

Weight (kDa)

8.91

Isoelectric Point (pI)

36.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 28 - 134 2.5e-25 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 185
AciI CCGC 3 cut(s) 180, 279, 449
AclI AACGTT 1 cut(s) 316
AclWI GGATC 1 cut(s) 315
AcsI RAATTY 1 cut(s) 394
AfaI GTAC 2 cut(s) 188, 433
AfiI CCNNNNNNNGG 1 cut(s) 179
AgsI TTSAA 1 cut(s) 125
AleI CACNNNNGTG 1 cut(s) 104
AlwI GGATC 1 cut(s) 315
AoxI GGCC 2 cut(s) 46, 276
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 394
AspA2I CCTAGG 1 cut(s) 43
AspLEI GCGC 1 cut(s) 185
AspS9I GGNCC 1 cut(s) 12
AsuHPI GGTGA 1 cut(s) 361
AvaII GGWCC 1 cut(s) 12
AvrII CCTAGG 1 cut(s) 43
BaeI ACNNNNGTAYC 2 cut(s) 369, 402
BccI CCATC 1 cut(s) 254
BceAI ACGGC 2 cut(s) 17, 370
BfaI CTAG 2 cut(s) 44, 104
BisI GCNGC 3 cut(s) 6, 181, 279
BlnI CCTAGG 1 cut(s) 43
BlsI GCNGC 3 cut(s) 7, 182, 280
Bme18I GGWCC 1 cut(s) 12
BmgT120I GGNCC 1 cut(s) 12
BmrI ACTGGG 1 cut(s) 447
BmsI GCATC 2 cut(s) 334, 366
BmuI ACTGGG 1 cut(s) 447
BsaJI CCNNGG 1 cut(s) 43
Bsc4I CCNNNNNNNGG 1 cut(s) 179
Bse1I ACTGG 1 cut(s) 453
Bse3DI GCAATG 1 cut(s) 130
BseDI CCNNGG 1 cut(s) 43
BseLI CCNNNNNNNGG 1 cut(s) 179
BseMI GCAATG 1 cut(s) 130
BseNI ACTGG 1 cut(s) 453
Bsh1236I CGCG 1 cut(s) 185
BshFI GGCC 2 cut(s) 48, 278
BslFI GGGAC 1 cut(s) 230
BslI CCNNNNNNNGG 1 cut(s) 179
BsmFI GGGAC 1 cut(s) 230
BsnI GGCC 2 cut(s) 48, 278
Bsp143I GATC 1 cut(s) 307
BspACI CCGC 3 cut(s) 180, 279, 449
BspANI GGCC 2 cut(s) 48, 278
BspFNI CGCG 1 cut(s) 185
BspPI GGATC 1 cut(s) 315
BsrDI GCAATG 1 cut(s) 130
BsrI ACTGG 1 cut(s) 453
BssECI CCNNGG 1 cut(s) 43
BssMI GATC 1 cut(s) 307
BssT1I CCWWGG 1 cut(s) 43
Bst4CI ACNGT 1 cut(s) 118
Bst6I CTCTTC 2 cut(s) 233, 469
BstC8I GCNNGC 2 cut(s) 39, 323
BstFNI CGCG 1 cut(s) 185
BstHHI GCGC 1 cut(s) 185
BstKTI GATC 1 cut(s) 310
BstMBI GATC 1 cut(s) 307
BstMWI GCNNNNNNNGC 1 cut(s) 38
BstNSI RCATGY 3 cut(s) 139, 244, 325
BstUI CGCG 1 cut(s) 185
BsuRI GGCC 2 cut(s) 48, 278
BtsI GCAGTG 1 cut(s) 251
BtsIMutI CAGTG 3 cut(s) 114, 251, 460
Cac8I GCNNGC 2 cut(s) 39, 323
CfoI GCGC 1 cut(s) 185
Cfr13I GGNCC 1 cut(s) 12
Csp6I GTAC 2 cut(s) 187, 432
CviAII CATG 4 cut(s) 136, 164, 241, 322
CviJI RGCY 5 cut(s) 5, 48, 278, 298, 474
CviKI_1 RGCY 5 cut(s) 5, 48, 278, 298, 474
CviQI GTAC 2 cut(s) 187, 432
DpnI GATC 1 cut(s) 309
DpnII GATC 1 cut(s) 307
Eam1104I CTCTTC 2 cut(s) 233, 469
EarI CTCTTC 2 cut(s) 233, 469
EciI GGCGGA 1 cut(s) 438
Eco130I CCWWGG 1 cut(s) 43
Eco47I GGWCC 1 cut(s) 12
EcoT14I CCWWGG 1 cut(s) 43
EcoT22I ATGCAT 1 cut(s) 327
ErhI CCWWGG 1 cut(s) 43
FaeI CATG 4 cut(s) 139, 167, 244, 325
FaiI YATR 8 cut(s) 84, 137, 165, 242, 290, 292, 323, 511
FaqI GGGAC 1 cut(s) 230
FatI CATG 4 cut(s) 135, 163, 240, 321
Fnu4HI GCNGC 3 cut(s) 6, 181, 279
Fsp4HI GCNGC 3 cut(s) 6, 181, 279
FspBI CTAG 2 cut(s) 44, 104
GlaI GCGC 1 cut(s) 184
GluI GCNGC 3 cut(s) 6, 181, 279
HaeIII GGCC 2 cut(s) 48, 278
HhaI GCGC 1 cut(s) 185
Hin1II CATG 4 cut(s) 139, 167, 244, 325
Hin6I GCGC 1 cut(s) 183
HinP1I GCGC 1 cut(s) 183
HphI GGTGA 1 cut(s) 361
Hpy166II GTNNAC 3 cut(s) 390, 434, 460
Hpy188I TCNGA 2 cut(s) 74, 259
Hpy8I GTNNAC 3 cut(s) 390, 434, 460
HpyAV CCTTC 3 cut(s) 246, 376, 376
HpyCH4III ACNGT 1 cut(s) 118
HpyCH4IV ACGT 3 cut(s) 90, 197, 316
HpyCH4V TGCA 7 cut(s) 8, 135, 244, 321, 325, 333, 493
HpyF10VI GCNNNNNNNGC 1 cut(s) 38
HpySE526I ACGT 3 cut(s) 90, 197, 316
Hsp92II CATG 4 cut(s) 139, 167, 244, 325
HspAI GCGC 1 cut(s) 183
Kzo9I GATC 1 cut(s) 307
LmnI GCTCC 1 cut(s) 451
LpnPI CCDG 2 cut(s) 199, 466
LweI GCATC 2 cut(s) 334, 366
MaeI CTAG 2 cut(s) 44, 104
MaeII ACGT 3 cut(s) 90, 197, 316
MalI GATC 1 cut(s) 309
MboI GATC 1 cut(s) 307
MboII GAAGA 2 cut(s) 220, 456
MluCI AATT 3 cut(s) 50, 168, 394
MnlI CCTC 3 cut(s) 236, 297, 412
Mph1103I ATGCAT 1 cut(s) 327
MseI TTAA 1 cut(s) 285
MslI CAYNNNNRTG 1 cut(s) 104
MspA1I CMGCKG 1 cut(s) 180
MvnI CGCG 1 cut(s) 185
MwoI GCNNNNNNNGC 1 cut(s) 38
NdeII GATC 1 cut(s) 307
NlaIII CATG 4 cut(s) 139, 167, 244, 325
NmeAIII GCCGAG 1 cut(s) 450
NsiI ATGCAT 1 cut(s) 327
NspI RCATGY 3 cut(s) 139, 244, 325
OliI CACNNNNGTG 1 cut(s) 104
PaeI GCATGC 1 cut(s) 325
PkrI GCNGC 3 cut(s) 7, 182, 280
Psp1406I AACGTT 1 cut(s) 316
PspPI GGNCC 1 cut(s) 12
RsaI GTAC 2 cut(s) 188, 433
RsaNI GTAC 2 cut(s) 187, 432
RseI CAYNNNNRTG 1 cut(s) 104
SaqAI TTAA 1 cut(s) 285
SatI GCNGC 3 cut(s) 6, 181, 279
Sau3AI GATC 1 cut(s) 307
Sau96I GGNCC 1 cut(s) 12
SetI ASST 5 cut(s) 93, 200, 257, 319, 387
SfaNI GCATC 2 cut(s) 334, 366
SinI GGWCC 1 cut(s) 12
SmiMI CAYNNNNRTG 1 cut(s) 104
SphI GCATGC 1 cut(s) 325
Sse9I AATT 3 cut(s) 50, 168, 394
SsiI CCGC 3 cut(s) 180, 279, 449
SspMI CTAG 2 cut(s) 44, 104
StyI CCWWGG 1 cut(s) 43
TaaI ACNGT 1 cut(s) 118
TaiI ACGT 3 cut(s) 93, 200, 319
TasI AATT 3 cut(s) 50, 168, 394
TatI WGTACW 1 cut(s) 431
TauI GCSGC 2 cut(s) 183, 281
Tru1I TTAA 1 cut(s) 285
Tru9I TTAA 1 cut(s) 285
TscAI CASTG 3 cut(s) 121, 251, 460
TseI GCWGC 1 cut(s) 5
TspDTI ATGAA 5 cut(s) 10, 48, 119, 363, 493
TspRI CASTG 3 cut(s) 121, 251, 460
VpaK11BI GGWCC 1 cut(s) 12
XapI RAATTY 1 cut(s) 394
XceI RCATGY 3 cut(s) 139, 244, 325
XmaJI CCTAGG 1 cut(s) 43
XspI CTAG 2 cut(s) 44, 104
Zsp2I ATGCAT 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.