RchiOBHm_Chr4g0396811

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0012568
Physical Location & Seq
Reverse (-)
12883332 .. 12883799
468 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36918

Sequence Viewer

Length: 468 bp
ATGCCTATGGTAGTTTGCAAGCATACAATTCTGAGAACAATCATGGGTGTAATATTTTGCTACCAAGCTTTGCTGCTCGCCTTGTTCATTGCTTCCTTTGTGGCGCTTACAACCGCTGTGGTACCCTTCAAAGTCGTGACCTCGATCGACAACACATTACCTGACGGGCAAGAACTCAATGTCCACTGTAAATCCGGGGACGACGATCTAGGCGCCCAGGTCATACCCAATGGAGGTCATTACGAATGGAGCTTCAAAGTTAATGTATGGGCAACAACGCTTTTCTTCTGTTCATTTAATTGCAAAGAAGGATCAGGAACATTTGACATCTACAGAGCCAGTCGGGATATCATTAGATGTGAAGACAAGTGCAACTGGAAAGCTGCCCAAGATGGCGTGCATGGCTTCAACAAAGACAATCAAGATGACTTTCTGTACAAGTGGGAGGCACCACAAGCGAAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

155

Amino Acids

17.36

Weight (kDa)

5.73

Isoelectric Point (pI)

19.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 49 - 148 1.7e-29 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 121
AccB1I GGYRCC 3 cut(s) 121, 212, 448
AciI CCGC 1 cut(s) 114
AclWI GGATC 1 cut(s) 319
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 2 cut(s) 123, 437
AfiI CCNNNNNNNGG 1 cut(s) 233
AgsI TTSAA 3 cut(s) 130, 256, 409
AjnI CCWGG 1 cut(s) 216
AloI GAACNNNNNNTCC 2 cut(s) 165, 197
AluBI AGCT 3 cut(s) 68, 252, 383
AluI AGCT 3 cut(s) 68, 252, 383
AlwI GGATC 1 cut(s) 319
ApeKI GCWGC 2 cut(s) 73, 383
Asp718I GGTACC 1 cut(s) 121
AspLEI GCGC 2 cut(s) 106, 215
AsuC2I CCSGG 1 cut(s) 196
BanI GGYRCC 3 cut(s) 121, 212, 448
BbsI GAAGAC 1 cut(s) 369
BbvI GCAGC 2 cut(s) 60, 370
BccI CCATC 1 cut(s) 386
BciT130I CCWGG 1 cut(s) 218
BcnI CCSGG 1 cut(s) 196
BfaI CTAG 1 cut(s) 209
BfmI CTRYAG 1 cut(s) 331
BfoI RGCGCY 2 cut(s) 107, 216
BisI GCNGC 2 cut(s) 74, 384
BlsI GCNGC 2 cut(s) 75, 385
Bme1390I CCNGG 2 cut(s) 196, 218
BmiI GGNNCC 3 cut(s) 123, 214, 450
BmrFI CCNGG 2 cut(s) 196, 218
BpiI GAAGAC 1 cut(s) 369
BpuMI CCSGG 1 cut(s) 196
BsaHI GRCGYC 1 cut(s) 213
BsaJI CCNNGG 2 cut(s) 195, 216
Bsc4I CCNNNNNNNGG 1 cut(s) 233
Bse1I ACTGG 2 cut(s) 339, 380
Bse3DI GCAATG 1 cut(s) 87
BseBI CCWGG 1 cut(s) 218
BseDI CCNNGG 2 cut(s) 195, 216
BseLI CCNNNNNNNGG 1 cut(s) 233
BseMI GCAATG 1 cut(s) 87
BseMII CTCAG 1 cut(s) 23
BseNI ACTGG 2 cut(s) 339, 380
BseXI GCAGC 2 cut(s) 60, 370
Bsh1285I CGRYCG 1 cut(s) 147
BshNI GGYRCC 3 cut(s) 121, 212, 448
BsiEI CGRYCG 1 cut(s) 147
BsiSI CCGG 1 cut(s) 195
BslFI GGGAC 1 cut(s) 212
BslI CCNNNNNNNGG 1 cut(s) 233
BsmFI GGGAC 1 cut(s) 212
Bsp1407I TGTACA 1 cut(s) 435
Bsp143I GATC 3 cut(s) 144, 205, 311
BspACI CCGC 1 cut(s) 114
BspCNI CTCAG 1 cut(s) 24
BspLI GGNNCC 3 cut(s) 123, 214, 450
BspPI GGATC 1 cut(s) 319
BspT107I GGYRCC 3 cut(s) 121, 212, 448
BsrDI GCAATG 1 cut(s) 87
BsrGI TGTACA 1 cut(s) 435
BsrI ACTGG 2 cut(s) 339, 380
BssECI CCNNGG 2 cut(s) 195, 216
BssMI GATC 3 cut(s) 144, 205, 311
BssNI GRCGYC 1 cut(s) 213
Bst2UI CCWGG 1 cut(s) 218
Bst4CI ACNGT 1 cut(s) 188
BstACI GRCGYC 1 cut(s) 213
BstAUI TGTACA 1 cut(s) 435
BstC8I GCNNGC 3 cut(s) 20, 78, 398
BstDEI CTNAG 1 cut(s) 32
BstH2I RGCGCY 2 cut(s) 107, 216
BstHHI GCGC 2 cut(s) 106, 215
BstKTI GATC 3 cut(s) 147, 208, 314
BstMBI GATC 3 cut(s) 144, 205, 311
BstMCI CGRYCG 1 cut(s) 147
BstMWI GCNNNNNNNGC 2 cut(s) 402, 455
BstNI CCWGG 1 cut(s) 218
BstSCI CCNGG 2 cut(s) 194, 216
BstSFI CTRYAG 1 cut(s) 331
BstV1I GCAGC 2 cut(s) 60, 370
BstV2I GAAGAC 1 cut(s) 369
BtsIMutI CAGTG 1 cut(s) 184
Cac8I GCNNGC 3 cut(s) 20, 78, 398
CfoI GCGC 2 cut(s) 106, 215
Csp6I GTAC 2 cut(s) 122, 436
CspCI CAANNNNNGTGG 2 cut(s) 99, 134
CviAII CATG 2 cut(s) 43, 401
CviJI RGCY 5 cut(s) 68, 252, 338, 383, 405
CviKI_1 RGCY 5 cut(s) 68, 252, 338, 383, 405
CviQI GTAC 2 cut(s) 122, 436
DdeI CTNAG 1 cut(s) 32
DinI GGCGCC 1 cut(s) 214
DpnI GATC 3 cut(s) 146, 207, 313
DpnII GATC 3 cut(s) 144, 205, 311
Eco32I GATATC 1 cut(s) 349
EcoRII CCWGG 1 cut(s) 216
EcoRV GATATC 1 cut(s) 349
EgeI GGCGCC 1 cut(s) 214
EheI GGCGCC 1 cut(s) 214
FaeI CATG 2 cut(s) 46, 404
FaiI YATR 7 cut(s) 8, 24, 44, 224, 268, 402, 466
FaqI GGGAC 1 cut(s) 212
FatI CATG 2 cut(s) 42, 400
Fnu4HI GCNGC 2 cut(s) 74, 384
Fsp4HI GCNGC 2 cut(s) 74, 384
FspBI CTAG 1 cut(s) 209
GlaI GCGC 2 cut(s) 105, 214
GluI GCNGC 2 cut(s) 74, 384
HaeII RGCGCY 2 cut(s) 107, 216
HapII CCGG 1 cut(s) 195
HhaI GCGC 2 cut(s) 106, 215
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 2 cut(s) 46, 404
Hin6I GCGC 2 cut(s) 104, 213
HinP1I GCGC 2 cut(s) 104, 213
HindIII AAGCTT 1 cut(s) 66
HpaII CCGG 1 cut(s) 195
Hpy166II GTNNAC 1 cut(s) 184
Hpy188I TCNGA 1 cut(s) 33
Hpy188III TCNNGA 4 cut(s) 136, 315, 344, 422
Hpy8I GTNNAC 1 cut(s) 184
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 2 cut(s) 136, 302
HpyCH4III ACNGT 1 cut(s) 188
HpyCH4V TGCA 4 cut(s) 18, 303, 372, 400
HpyF10VI GCNNNNNNNGC 2 cut(s) 402, 455
HpyF3I CTNAG 1 cut(s) 32
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 2 cut(s) 46, 404
HspAI GCGC 2 cut(s) 104, 213
KasI GGCGCC 1 cut(s) 212
KpnI GGTACC 1 cut(s) 125
Kzo9I GATC 3 cut(s) 144, 205, 311
LmnI GCTCC 1 cut(s) 249
LpnPI CCDG 7 cut(s) 174, 203, 208, 230, 300, 352, 361
Lsp1109I GCAGC 2 cut(s) 60, 370
MaeI CTAG 1 cut(s) 209
MaeIII GTNAC 1 cut(s) 136
MalI GATC 3 cut(s) 146, 207, 313
MboI GATC 3 cut(s) 144, 205, 311
MboII GAAGA 2 cut(s) 277, 374
MluCI AATT 2 cut(s) 27, 298
Mly113I GGCGCC 1 cut(s) 213
MnlI CCTC 3 cut(s) 151, 227, 439
MseI TTAA 2 cut(s) 261, 297
MspA1I CMGCKG 1 cut(s) 116
MspI CCGG 1 cut(s) 195
MspR9I CCNGG 2 cut(s) 196, 218
MvaI CCWGG 1 cut(s) 218
MwoI GCNNNNNNNGC 2 cut(s) 402, 455
NarI GGCGCC 1 cut(s) 213
NciI CCSGG 1 cut(s) 196
NdeII GATC 3 cut(s) 144, 205, 311
NlaIII CATG 2 cut(s) 46, 404
NlaIV GGNNCC 3 cut(s) 123, 214, 450
NmuCI GTSAC 1 cut(s) 136
PkrI GCNGC 2 cut(s) 75, 385
Ple19I CGATCG 1 cut(s) 147
PluTI GGCGCC 1 cut(s) 216
Psp6I CCWGG 1 cut(s) 216
PspGI CCWGG 1 cut(s) 216
PspN4I GGNNCC 3 cut(s) 123, 214, 450
PvuI CGATCG 1 cut(s) 147
RsaI GTAC 2 cut(s) 123, 437
RsaNI GTAC 2 cut(s) 122, 436
SaqAI TTAA 2 cut(s) 261, 297
SatI GCNGC 2 cut(s) 74, 384
Sau3AI GATC 3 cut(s) 144, 205, 311
ScrFI CCNGG 2 cut(s) 196, 218
SetI ASST 7 cut(s) 70, 143, 163, 222, 238, 254, 385
SfcI CTRYAG 1 cut(s) 331
SfoI GGCGCC 1 cut(s) 214
Sse9I AATT 2 cut(s) 27, 298
SsiI CCGC 1 cut(s) 114
SspDI GGCGCC 1 cut(s) 212
SspI AATATT 1 cut(s) 54
SspMI CTAG 1 cut(s) 209
StyD4I CCNGG 2 cut(s) 194, 216
TaaI ACNGT 1 cut(s) 188
TaqI TCGA 2 cut(s) 143, 147
TasI AATT 2 cut(s) 27, 298
TatI WGTACW 1 cut(s) 435
Tru1I TTAA 2 cut(s) 261, 297
Tru9I TTAA 2 cut(s) 261, 297
TscAI CASTG 1 cut(s) 191
TseFI GTSAC 1 cut(s) 136
TseI GCWGC 2 cut(s) 73, 383
Tsp45I GTSAC 1 cut(s) 136
TspDTI ATGAA 2 cut(s) 76, 282
TspRI CASTG 1 cut(s) 191
XspI CTAG 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.