MD05G1010100.v1.1

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0012568
Physical Location & Seq
Reverse (-)
2086312 .. 2086725
414 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1010100.v1.1.491

Sequence Viewer

Length: 414 bp
ATGGCTGTGCATATTAGAAGTGCAGTGGTGTTAACATTTCTTCTGATGCTAACAAGTACATGTTACGCCGATATCATCAATCCCATAACTCATGTCACAATTATTAACTATTTACGTAATGGTCGCGACCTTACAGTTCACTGTAAGTCCGCGGATGATGATGTTGGTGTTCATGTGATACCCCCTAATGGGTCCTTTGAGTTCCATTTTCGACCAAATTTTTGGAATTCAACGCAATATTATTGCAAATTTGACTGGGAAGGCGGATCTTACTGGTTTGACATATACATACAACTTAGAGACAAGCCTAACTGCGTGAAGAATTGTAATTGGCTCATCATACCAAATGGTGCATGCAGGCAAATTGAAGGAAGGCCAGATTTGGTGGACAGGTGCTATCCTTGGAATTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

138

Amino Acids

15.95

Weight (kDa)

6.48

Isoelectric Point (pI)

26.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 31 - 135 7e-29 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 126, 152
AciI CCGC 3 cut(s) 150, 152, 264
AclWI GGATC 1 cut(s) 274
AcsI RAATTY 4 cut(s) 217, 226, 248, 406
AfaI GTAC 1 cut(s) 58
AfiI CCNNNNNNNGG 2 cut(s) 188, 189
AflIII ACRYGT 1 cut(s) 59
AgsI TTSAA 2 cut(s) 231, 368
Alw26I GTCTC 1 cut(s) 294
AlwI GGATC 1 cut(s) 274
AoxI GGCC 1 cut(s) 374
ApoI RAATTY 4 cut(s) 217, 226, 248, 406
ArsI GACNNNNNNTTYG 2 cut(s) 204, 236
AspS9I GGNCC 1 cut(s) 192
AvaII GGWCC 1 cut(s) 192
BcoDI GTCTC 1 cut(s) 294
Bme18I GGWCC 1 cut(s) 192
BmgT120I GGNCC 1 cut(s) 192
BmiI GGNNCC 1 cut(s) 193
BmrI ACTGGG 1 cut(s) 265
BmsI GCATC 1 cut(s) 36
BmuI ACTGGG 1 cut(s) 265
BsaAI YACGTR 1 cut(s) 116
BsaJI CCNNGG 2 cut(s) 150, 401
Bsc4I CCNNNNNNNGG 2 cut(s) 188, 189
Bse1I ACTGG 2 cut(s) 260, 278
BseDI CCNNGG 2 cut(s) 150, 401
BseGI GGATG 1 cut(s) 160
BseLI CCNNNNNNNGG 2 cut(s) 188, 189
BseNI ACTGG 2 cut(s) 260, 278
BsgI GTGCAG 1 cut(s) 42
Bsh1236I CGCG 2 cut(s) 126, 152
BshFI GGCC 1 cut(s) 376
BslI CCNNNNNNNGG 2 cut(s) 188, 189
BsmAI GTCTC 1 cut(s) 294
BsnI GGCC 1 cut(s) 376
Bsp143I GATC 1 cut(s) 266
Bsp68I TCGCGA 1 cut(s) 126
BspACI CCGC 3 cut(s) 150, 152, 264
BspANI GGCC 1 cut(s) 376
BspFNI CGCG 2 cut(s) 126, 152
BspLI GGNNCC 1 cut(s) 193
BspPI GGATC 1 cut(s) 274
BsrI ACTGG 2 cut(s) 260, 278
BssECI CCNNGG 2 cut(s) 150, 401
BssMI GATC 1 cut(s) 266
BssT1I CCWWGG 1 cut(s) 401
Bst4CI ACNGT 2 cut(s) 136, 143
BstBAI YACGTR 1 cut(s) 116
BstC8I GCNNGC 2 cut(s) 355, 359
BstDEI CTNAG 1 cut(s) 296
BstDSI CCRYGG 1 cut(s) 150
BstF5I GGATG 1 cut(s) 160
BstFNI CGCG 2 cut(s) 126, 152
BstKTI GATC 1 cut(s) 269
BstMAI GTCTC 1 cut(s) 294
BstMBI GATC 1 cut(s) 266
BstNSI RCATGY 2 cut(s) 63, 357
BstSNI TACGTA 1 cut(s) 116
BstUI CGCG 2 cut(s) 126, 152
BstX2I RGATCY 1 cut(s) 266
BstXI CCANNNNNNTGG 1 cut(s) 222
BstYI RGATCY 1 cut(s) 266
BsuRI GGCC 1 cut(s) 376
BtgI CCRYGG 1 cut(s) 150
BtsCI GGATG 1 cut(s) 160
BtsI GCAGTG 1 cut(s) 30
BtsIMutI CAGTG 2 cut(s) 30, 139
BtuMI TCGCGA 1 cut(s) 126
Cac8I GCNNGC 2 cut(s) 355, 359
Cfr13I GGNCC 1 cut(s) 192
Cfr42I CCGCGG 1 cut(s) 153
Csp6I GTAC 1 cut(s) 57
CviAII CATG 4 cut(s) 60, 92, 173, 354
CviJI RGCY 4 cut(s) 5, 307, 334, 376
CviKI_1 RGCY 4 cut(s) 5, 307, 334, 376
CviQI GTAC 1 cut(s) 57
DdeI CTNAG 1 cut(s) 296
DpnI GATC 1 cut(s) 268
DpnII GATC 1 cut(s) 266
EciI GGCGGA 1 cut(s) 279
Eco105I TACGTA 1 cut(s) 116
Eco130I CCWWGG 1 cut(s) 401
Eco32I GATATC 1 cut(s) 73
Eco47I GGWCC 1 cut(s) 192
EcoO109I RGGNCCY 1 cut(s) 192
EcoRI GAATTC 2 cut(s) 226, 406
EcoRV GATATC 1 cut(s) 73
EcoT14I CCWWGG 1 cut(s) 401
ErhI CCWWGG 1 cut(s) 401
FaeI CATG 4 cut(s) 63, 95, 176, 357
FatI CATG 4 cut(s) 59, 91, 172, 353
FokI GGATG 1 cut(s) 167
HaeIII GGCC 1 cut(s) 376
Hin1II CATG 4 cut(s) 63, 95, 176, 357
HincII GTYRAC 1 cut(s) 33
HindII GTYRAC 1 cut(s) 33
HpaI GTTAAC 1 cut(s) 33
Hpy166II GTNNAC 3 cut(s) 33, 139, 388
Hpy188I TCNGA 1 cut(s) 45
Hpy188III TCNNGA 1 cut(s) 125
Hpy8I GTNNAC 3 cut(s) 33, 139, 388
HpyAV CCTTC 3 cut(s) 254, 362, 366
HpyCH4III ACNGT 2 cut(s) 136, 143
HpyCH4IV ACGT 1 cut(s) 115
HpyCH4V TGCA 5 cut(s) 10, 23, 246, 353, 357
HpyF3I CTNAG 1 cut(s) 296
HpySE526I ACGT 1 cut(s) 115
Hsp92II CATG 4 cut(s) 63, 95, 176, 357
KspAI GTTAAC 1 cut(s) 33
KspI CCGCGG 1 cut(s) 153
Kzo9I GATC 1 cut(s) 266
LpnPI CCDG 5 cut(s) 241, 259, 343, 376, 390
LweI GCATC 1 cut(s) 36
MaeII ACGT 1 cut(s) 115
MaeIII GTNAC 2 cut(s) 62, 94
MalI GATC 1 cut(s) 268
MboI GATC 1 cut(s) 266
MboII GAAGA 2 cut(s) 32, 331
MflI RGATCY 1 cut(s) 266
MluCI AATT 8 cut(s) 99, 217, 226, 248, 322, 328, 363, 406
MseI TTAA 2 cut(s) 32, 105
MspA1I CMGCKG 1 cut(s) 152
MvnI CGCG 2 cut(s) 126, 152
NdeII GATC 1 cut(s) 266
NlaIII CATG 4 cut(s) 63, 95, 176, 357
NlaIV GGNNCC 1 cut(s) 193
NmuCI GTSAC 1 cut(s) 94
NruI TCGCGA 1 cut(s) 126
NspI RCATGY 2 cut(s) 63, 357
PaeI GCATGC 1 cut(s) 357
PciI ACATGT 1 cut(s) 59
Ppu21I YACGTR 1 cut(s) 116
PpuMI RGGWCCY 1 cut(s) 192
PscI ACATGT 1 cut(s) 59
Psp5II RGGWCCY 1 cut(s) 192
PspN4I GGNNCC 1 cut(s) 193
PspPI GGNCC 1 cut(s) 192
PspPPI RGGWCCY 1 cut(s) 192
PsuI RGATCY 1 cut(s) 266
RruI TCGCGA 1 cut(s) 126
RsaI GTAC 1 cut(s) 58
RsaNI GTAC 1 cut(s) 57
SacII CCGCGG 1 cut(s) 153
SaqAI TTAA 2 cut(s) 32, 105
Sau3AI GATC 1 cut(s) 266
Sau96I GGNCC 1 cut(s) 192
SetI ASST 3 cut(s) 118, 132, 395
SfaNI GCATC 1 cut(s) 36
Sfr303I CCGCGG 1 cut(s) 153
SgrBI CCGCGG 1 cut(s) 153
SinI GGWCC 1 cut(s) 192
SnaBI TACGTA 1 cut(s) 116
SphI GCATGC 1 cut(s) 357
Sse9I AATT 8 cut(s) 99, 217, 226, 248, 322, 328, 363, 406
SsiI CCGC 3 cut(s) 150, 152, 264
SspI AATATT 1 cut(s) 239
StyI CCWWGG 1 cut(s) 401
TaaI ACNGT 2 cut(s) 136, 143
TaiI ACGT 1 cut(s) 118
TaqI TCGA 1 cut(s) 211
TasI AATT 8 cut(s) 99, 217, 226, 248, 322, 328, 363, 406
TatI WGTACW 1 cut(s) 56
Tru1I TTAA 2 cut(s) 32, 105
Tru9I TTAA 2 cut(s) 32, 105
TscAI CASTG 2 cut(s) 30, 146
TseFI GTSAC 1 cut(s) 94
Tsp45I GTSAC 1 cut(s) 94
TspDTI ATGAA 2 cut(s) 161, 399
TspRI CASTG 2 cut(s) 30, 146
VpaK11BI GGWCC 1 cut(s) 192
XapI RAATTY 4 cut(s) 217, 226, 248, 406
XceI RCATGY 2 cut(s) 63, 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.