MD00G1132500.v1.1

Plant self-incompatibility protein S1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0012568
Physical Location & Seq
Reverse (-)
28660056 .. 28660493
438 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1132500.v1.1.491

Sequence Viewer

Length: 438 bp
ATGGTTGCGAGTAGCCGTATTATGTTCGTTCTTTTTGCCGTGGTGGTACTCACACTGGGCCAATCTTCACGTCCGGTCTCGGCATGGCTGGCCGGGGAGGTCCTTCTCGAGATACAAAACGTTATGGACCCTTCGAAGGCTAAAATCGAAGTTCATTGCAAGTCCGGGGACGACGATCTGCAAAACCATGTACTCGAATATGGCAAGATTTACAGCTTCAAATTCGAGAATAACGCTCTGGACACAACCCTTTTCTATTGCTACTTTCAGTGGAAAAATCCTGAGGGAAAATGGATCCAAACCTCCCATGACATATACGACGCCAAGAGGGATCAAGAACGTTGCATGCATCATTGCCAGTGGCGGGTTGCAGAAGAAGGCTTCTTTTCTTACAGCATAAAGAACGACGTTTGGGAAAACCTCTACAATTGGACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

146

Amino Acids

17.04

Weight (kDa)

5.47

Isoelectric Point (pI)

38.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 36 - 144 1.2e-24 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 364
AclI AACGTT 2 cut(s) 120, 340
AclWI GGATC 3 cut(s) 289, 302, 339
AcoI YGGCCR 1 cut(s) 90
AcsI RAATTY 1 cut(s) 221
AcyI GRCGYC 1 cut(s) 321
AfaI GTAC 2 cut(s) 48, 192
AfiI CCNNNNNNNGG 2 cut(s) 136, 364
AgsI TTSAA 1 cut(s) 220
AjiI CACGTC 1 cut(s) 71
AluBI AGCT 1 cut(s) 216
AluI AGCT 1 cut(s) 216
Alw26I GTCTC 1 cut(s) 82
AlwI GGATC 3 cut(s) 289, 302, 339
Ama87I CYCGRG 1 cut(s) 107
AoxI GGCC 2 cut(s) 58, 90
ApoI RAATTY 1 cut(s) 221
AspS9I GGNCC 4 cut(s) 58, 100, 127, 432
AsuC2I CCSGG 2 cut(s) 94, 166
AsuII TTCGAA 1 cut(s) 134
AvaI CYCGRG 1 cut(s) 107
AvaII GGWCC 3 cut(s) 100, 127, 432
AxyI CCTNAGG 1 cut(s) 282
BamHI GGATCC 1 cut(s) 294
BceAI ACGGC 1 cut(s) 23
BcnI CCSGG 2 cut(s) 94, 166
BcoDI GTCTC 1 cut(s) 82
Bme1390I CCNGG 2 cut(s) 94, 166
Bme18I GGWCC 3 cut(s) 100, 127, 432
BmeT110I CYCGRG 1 cut(s) 107
BmgBI CACGTC 1 cut(s) 71
BmgT120I GGNCC 4 cut(s) 58, 100, 127, 432
BmiI GGNNCC 2 cut(s) 129, 296
BmrFI CCNGG 2 cut(s) 94, 166
BmrI ACTGGG 1 cut(s) 65
BmsI GCATC 1 cut(s) 358
BmuI ACTGGG 1 cut(s) 65
Bpu14I TTCGAA 1 cut(s) 134
BpuMI CCSGG 2 cut(s) 94, 166
BsaHI GRCGYC 1 cut(s) 321
BsaI GGTCTC 1 cut(s) 82
BsaJI CCNNGG 3 cut(s) 39, 93, 165
BsaWI WCCGGW 1 cut(s) 73
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 364
Bse1I ACTGG 2 cut(s) 60, 358
Bse21I CCTNAGG 1 cut(s) 282
Bse3DI GCAATG 2 cut(s) 154, 352
BseDI CCNNGG 3 cut(s) 39, 93, 165
BseLI CCNNNNNNNGG 2 cut(s) 136, 364
BseMI GCAATG 2 cut(s) 154, 352
BseMII CTCAG 1 cut(s) 273
BseNI ACTGG 2 cut(s) 60, 358
BshFI GGCC 2 cut(s) 60, 92
BsiHKCI CYCGRG 1 cut(s) 107
BsiSI CCGG 3 cut(s) 74, 93, 165
BslFI GGGAC 1 cut(s) 182
BslI CCNNNNNNNGG 2 cut(s) 136, 364
BsmAI GTCTC 1 cut(s) 82
BsmFI GGGAC 1 cut(s) 182
BsnI GGCC 2 cut(s) 60, 92
Bso31I GGTCTC 1 cut(s) 82
BsoBI CYCGRG 1 cut(s) 107
Bsp119I TTCGAA 1 cut(s) 134
Bsp143I GATC 3 cut(s) 175, 294, 331
BspACI CCGC 1 cut(s) 364
BspANI GGCC 2 cut(s) 60, 92
BspCNI CTCAG 1 cut(s) 274
BspLI GGNNCC 2 cut(s) 129, 296
BspPI GGATC 3 cut(s) 289, 302, 339
BspT104I TTCGAA 1 cut(s) 134
BspTNI GGTCTC 1 cut(s) 82
BsrDI GCAATG 2 cut(s) 154, 352
BsrI ACTGG 2 cut(s) 60, 358
BssECI CCNNGG 3 cut(s) 39, 93, 165
BssMI GATC 3 cut(s) 175, 294, 331
BssNI GRCGYC 1 cut(s) 321
BstACI GRCGYC 1 cut(s) 321
BstBI TTCGAA 1 cut(s) 134
BstC8I GCNNGC 2 cut(s) 90, 347
BstDEI CTNAG 1 cut(s) 282
BstDSI CCRYGG 1 cut(s) 39
BstKTI GATC 3 cut(s) 178, 297, 334
BstMAI GTCTC 1 cut(s) 82
BstMBI GATC 3 cut(s) 175, 294, 331
BstMWI GCNNNNNNNGC 1 cut(s) 89
BstNSI RCATGY 1 cut(s) 349
BstSCI CCNGG 2 cut(s) 92, 164
BstX2I RGATCY 1 cut(s) 294
BstYI RGATCY 1 cut(s) 294
Bsu36I CCTNAGG 1 cut(s) 282
BsuRI GGCC 2 cut(s) 60, 92
BtgI CCRYGG 1 cut(s) 39
BtrI CACGTC 1 cut(s) 71
BtsIMutI CAGTG 3 cut(s) 53, 275, 365
Cac8I GCNNGC 2 cut(s) 90, 347
Cfr13I GGNCC 4 cut(s) 58, 100, 127, 432
CseI GACGC 1 cut(s) 329
Csp6I GTAC 2 cut(s) 47, 191
CviAII CATG 4 cut(s) 84, 188, 308, 346
CviJI RGCY 7 cut(s) 15, 60, 88, 92, 140, 216, 381
CviKI_1 RGCY 7 cut(s) 15, 60, 88, 92, 140, 216, 381
CviQI GTAC 2 cut(s) 47, 191
DdeI CTNAG 1 cut(s) 282
DpnI GATC 3 cut(s) 177, 296, 333
DpnII GATC 3 cut(s) 175, 294, 331
EaeI YGGCCR 1 cut(s) 90
Eco31I GGTCTC 1 cut(s) 82
Eco47I GGWCC 3 cut(s) 100, 127, 432
Eco81I CCTNAGG 1 cut(s) 282
Eco88I CYCGRG 1 cut(s) 107
EcoO109I RGGNCCY 1 cut(s) 100
EcoT22I ATGCAT 1 cut(s) 351
FaeI CATG 4 cut(s) 87, 191, 311, 349
FaqI GGGAC 1 cut(s) 182
FatI CATG 4 cut(s) 83, 187, 307, 345
FauI CCCGC 1 cut(s) 357
HaeIII GGCC 2 cut(s) 60, 92
HapII CCGG 3 cut(s) 74, 93, 165
HgaI GACGC 1 cut(s) 329
Hin1I GRCGYC 1 cut(s) 321
Hin1II CATG 4 cut(s) 87, 191, 311, 349
HpaII CCGG 3 cut(s) 74, 93, 165
Hpy188III TCNNGA 6 cut(s) 107, 109, 226, 239, 281, 335
Hpy99I CGWCG 3 cut(s) 176, 323, 410
HpyAV CCTTC 4 cut(s) 113, 130, 141, 371
HpyCH4IV ACGT 4 cut(s) 70, 120, 340, 408
HpyCH4V TGCA 5 cut(s) 159, 181, 345, 349, 371
HpyF10VI GCNNNNNNNGC 1 cut(s) 89
HpyF3I CTNAG 1 cut(s) 282
HpySE526I ACGT 4 cut(s) 70, 120, 340, 408
Hsp92I GRCGYC 1 cut(s) 321
Hsp92II CATG 4 cut(s) 87, 191, 311, 349
Kzo9I GATC 3 cut(s) 175, 294, 331
LpnPI CCDG 8 cut(s) 41, 74, 87, 106, 178, 224, 294, 371
LweI GCATC 1 cut(s) 358
MaeII ACGT 4 cut(s) 70, 120, 340, 408
MalI GATC 3 cut(s) 177, 296, 333
MboI GATC 3 cut(s) 175, 294, 331
MboII GAAGA 2 cut(s) 57, 386
MfeI CAATTG 1 cut(s) 427
MflI RGATCY 1 cut(s) 294
MluCI AATT 2 cut(s) 221, 427
MnlI CCTC 5 cut(s) 91, 277, 313, 321, 431
Mph1103I ATGCAT 1 cut(s) 351
MspI CCGG 3 cut(s) 74, 93, 165
MspR9I CCNGG 2 cut(s) 94, 166
MunI CAATTG 1 cut(s) 427
MwoI GCNNNNNNNGC 1 cut(s) 89
NciI CCSGG 2 cut(s) 94, 166
NdeII GATC 3 cut(s) 175, 294, 331
NlaIII CATG 4 cut(s) 87, 191, 311, 349
NlaIV GGNNCC 2 cut(s) 129, 296
NmeAIII GCCGAG 1 cut(s) 59
NsiI ATGCAT 1 cut(s) 351
NspI RCATGY 1 cut(s) 349
NspV TTCGAA 1 cut(s) 134
PaeI GCATGC 1 cut(s) 349
PaeR7I CTCGAG 1 cut(s) 107
PpuMI RGGWCCY 1 cut(s) 100
Psp1406I AACGTT 2 cut(s) 120, 340
Psp5II RGGWCCY 1 cut(s) 100
PspN4I GGNNCC 2 cut(s) 129, 296
PspPI GGNCC 4 cut(s) 58, 100, 127, 432
PspPPI RGGWCCY 1 cut(s) 100
PsuI RGATCY 1 cut(s) 294
RsaI GTAC 2 cut(s) 48, 192
RsaNI GTAC 2 cut(s) 47, 191
Sau3AI GATC 3 cut(s) 175, 294, 331
Sau96I GGNCC 4 cut(s) 58, 100, 127, 432
ScrFI CCNGG 2 cut(s) 94, 166
SetI ASST 9 cut(s) 73, 102, 123, 218, 305, 343, 411, 423, 437
SfaNI GCATC 1 cut(s) 358
Sfr274I CTCGAG 1 cut(s) 107
SfuI TTCGAA 1 cut(s) 134
SinI GGWCC 3 cut(s) 100, 127, 432
SlaI CTCGAG 1 cut(s) 107
SmlI CTYRAG 1 cut(s) 107
SmoI CTYRAG 1 cut(s) 107
SphI GCATGC 1 cut(s) 349
Sse9I AATT 2 cut(s) 221, 427
SsiI CCGC 1 cut(s) 364
StyD4I CCNGG 2 cut(s) 92, 164
TaiI ACGT 4 cut(s) 73, 123, 343, 411
TaqI TCGA 5 cut(s) 108, 134, 147, 195, 225
TasI AATT 2 cut(s) 221, 427
TatI WGTACW 1 cut(s) 190
TscAI CASTG 3 cut(s) 60, 275, 365
TspDTI ATGAA 1 cut(s) 143
TspRI CASTG 3 cut(s) 60, 275, 365
VpaK11BI GGWCC 3 cut(s) 100, 127, 432
XapI RAATTY 1 cut(s) 221
XceI RCATGY 1 cut(s) 349
XhoI CTCGAG 1 cut(s) 107
Zsp2I ATGCAT 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.