Rh4DG067300

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0012568
Physical Location & Seq
Reverse (-)
10088084 .. 10088509
426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG067300.1

Sequence Viewer

Length: 426 bp
ATGGGTTTAATATTTCGCTACCAAGCTTTGCTGCTCGCCTTGTTCATTGCTTTCTTTGTGGCGCTTACAACTGCTGTGGTACCCTTCAAAGTCGTGACCTCGATCGACAACACATTACCTGATGGGCAAGAACTCAATGTCCACTGTAAATCCGGGGACGACGATCTAGGCGCTCAGGTCATACCCAATGGAGGTCATTACGAATGGAGCTTCAAAGTTAATGTATGGGCAACAACGCTTTTCTTCTGTTCATTTAATTGCAAAGACGGATCAGGAACATTTGACATCTACAGAGCCAGTCGGGATATCATTAGATGTGAAGACAAGTGCAACTGGAAAGCTGCCCAAGATGGCGTGCATGGCTTCAACAAAGACAATCAGGATGACTTTCTGTACAAGTGGGAGGCACCACAAGTGAAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

141

Amino Acids

15.91

Weight (kDa)

5.21

Isoelectric Point (pI)

13.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 35 - 134 1.3e-29 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 79
AccB1I GGYRCC 2 cut(s) 79, 406
AclWI GGATC 1 cut(s) 277
AfaI GTAC 2 cut(s) 81, 395
AfiI CCNNNNNNNGG 1 cut(s) 191
AgsI TTSAA 3 cut(s) 88, 214, 367
AloI GAACNNNNNNTCC 2 cut(s) 123, 155
AluBI AGCT 3 cut(s) 26, 210, 341
AluI AGCT 3 cut(s) 26, 210, 341
AlwI GGATC 1 cut(s) 277
ApeKI GCWGC 2 cut(s) 31, 341
Asp718I GGTACC 1 cut(s) 79
AspLEI GCGC 2 cut(s) 64, 173
AsuC2I CCSGG 1 cut(s) 154
BanI GGYRCC 2 cut(s) 79, 406
BbsI GAAGAC 1 cut(s) 327
BbvI GCAGC 2 cut(s) 18, 328
BccI CCATC 2 cut(s) 116, 344
BcnI CCSGG 1 cut(s) 154
BfaI CTAG 1 cut(s) 167
BfmI CTRYAG 1 cut(s) 289
BfoI RGCGCY 2 cut(s) 65, 174
BisI GCNGC 2 cut(s) 32, 342
BlsI GCNGC 2 cut(s) 33, 343
Bme1390I CCNGG 1 cut(s) 154
BmiI GGNNCC 2 cut(s) 81, 408
BmrFI CCNGG 1 cut(s) 154
BpiI GAAGAC 1 cut(s) 327
Bpu10I CCTNAGC 1 cut(s) 174
BpuMI CCSGG 1 cut(s) 154
BsaJI CCNNGG 1 cut(s) 153
Bsc4I CCNNNNNNNGG 1 cut(s) 191
Bse1I ACTGG 2 cut(s) 297, 338
Bse3DI GCAATG 1 cut(s) 45
BseDI CCNNGG 1 cut(s) 153
BseGI GGATG 1 cut(s) 388
BseLI CCNNNNNNNGG 1 cut(s) 191
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 1 cut(s) 188
BseNI ACTGG 2 cut(s) 297, 338
BseXI GCAGC 2 cut(s) 18, 328
Bsh1285I CGRYCG 1 cut(s) 105
BshNI GGYRCC 2 cut(s) 79, 406
BsiEI CGRYCG 1 cut(s) 105
BsiSI CCGG 1 cut(s) 153
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 1 cut(s) 191
BsmFI GGGAC 1 cut(s) 170
Bsp1407I TGTACA 1 cut(s) 393
Bsp143I GATC 3 cut(s) 102, 163, 269
BspCNI CTCAG 1 cut(s) 187
BspLI GGNNCC 2 cut(s) 81, 408
BspPI GGATC 1 cut(s) 277
BspT107I GGYRCC 2 cut(s) 79, 406
BsrDI GCAATG 1 cut(s) 45
BsrGI TGTACA 1 cut(s) 393
BsrI ACTGG 2 cut(s) 297, 338
BssECI CCNNGG 1 cut(s) 153
BssMI GATC 3 cut(s) 102, 163, 269
Bst4CI ACNGT 1 cut(s) 146
BstAUI TGTACA 1 cut(s) 393
BstC8I GCNNGC 2 cut(s) 36, 356
BstDEI CTNAG 1 cut(s) 174
BstF5I GGATG 1 cut(s) 388
BstH2I RGCGCY 2 cut(s) 65, 174
BstHHI GCGC 2 cut(s) 64, 173
BstKTI GATC 3 cut(s) 105, 166, 272
BstMBI GATC 3 cut(s) 102, 163, 269
BstMCI CGRYCG 1 cut(s) 105
BstMWI GCNNNNNNNGC 1 cut(s) 360
BstSCI CCNGG 1 cut(s) 152
BstSFI CTRYAG 1 cut(s) 289
BstV1I GCAGC 2 cut(s) 18, 328
BstV2I GAAGAC 1 cut(s) 327
BtsCI GGATG 1 cut(s) 388
BtsIMutI CAGTG 1 cut(s) 142
Cac8I GCNNGC 2 cut(s) 36, 356
CfoI GCGC 2 cut(s) 64, 173
Csp6I GTAC 2 cut(s) 80, 394
CspCI CAANNNNNGTGG 2 cut(s) 57, 92
CviAII CATG 1 cut(s) 359
CviJI RGCY 5 cut(s) 26, 210, 296, 341, 363
CviKI_1 RGCY 5 cut(s) 26, 210, 296, 341, 363
CviQI GTAC 2 cut(s) 80, 394
DdeI CTNAG 1 cut(s) 174
DpnI GATC 3 cut(s) 104, 165, 271
DpnII GATC 3 cut(s) 102, 163, 269
Eco32I GATATC 1 cut(s) 307
EcoRV GATATC 1 cut(s) 307
FaeI CATG 1 cut(s) 362
FaiI YATR 4 cut(s) 182, 226, 360, 424
FaqI GGGAC 1 cut(s) 170
FatI CATG 1 cut(s) 358
Fnu4HI GCNGC 2 cut(s) 32, 342
FokI GGATG 1 cut(s) 395
Fsp4HI GCNGC 2 cut(s) 32, 342
FspBI CTAG 1 cut(s) 167
GlaI GCGC 2 cut(s) 63, 172
GluI GCNGC 2 cut(s) 32, 342
HaeII RGCGCY 2 cut(s) 65, 174
HapII CCGG 1 cut(s) 153
HhaI GCGC 2 cut(s) 64, 173
Hin1II CATG 1 cut(s) 362
Hin6I GCGC 2 cut(s) 62, 171
HinP1I GCGC 2 cut(s) 62, 171
HindIII AAGCTT 1 cut(s) 24
HpaII CCGG 1 cut(s) 153
Hpy166II GTNNAC 1 cut(s) 142
Hpy188III TCNNGA 4 cut(s) 94, 273, 302, 380
Hpy8I GTNNAC 1 cut(s) 142
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 1 cut(s) 94
HpyCH4III ACNGT 1 cut(s) 146
HpyCH4V TGCA 3 cut(s) 261, 330, 358
HpyF10VI GCNNNNNNNGC 1 cut(s) 360
HpyF3I CTNAG 1 cut(s) 174
Hsp92II CATG 1 cut(s) 362
HspAI GCGC 2 cut(s) 62, 171
KpnI GGTACC 1 cut(s) 83
Kzo9I GATC 3 cut(s) 102, 163, 269
LmnI GCTCC 1 cut(s) 207
LpnPI CCDG 7 cut(s) 132, 161, 166, 258, 310, 319, 365
Lsp1109I GCAGC 2 cut(s) 18, 328
MaeI CTAG 1 cut(s) 167
MaeIII GTNAC 1 cut(s) 94
MalI GATC 3 cut(s) 104, 165, 271
MboI GATC 3 cut(s) 102, 163, 269
MboII GAAGA 2 cut(s) 235, 332
MluCI AATT 1 cut(s) 256
MnlI CCTC 3 cut(s) 109, 185, 397
MseI TTAA 3 cut(s) 8, 219, 255
MspI CCGG 1 cut(s) 153
MspR9I CCNGG 1 cut(s) 154
MwoI GCNNNNNNNGC 1 cut(s) 360
NciI CCSGG 1 cut(s) 154
NdeII GATC 3 cut(s) 102, 163, 269
NlaIII CATG 1 cut(s) 362
NlaIV GGNNCC 2 cut(s) 81, 408
NmuCI GTSAC 1 cut(s) 94
PkrI GCNGC 2 cut(s) 33, 343
Ple19I CGATCG 1 cut(s) 105
PspN4I GGNNCC 2 cut(s) 81, 408
PvuI CGATCG 1 cut(s) 105
RsaI GTAC 2 cut(s) 81, 395
RsaNI GTAC 2 cut(s) 80, 394
SaqAI TTAA 3 cut(s) 8, 219, 255
SatI GCNGC 2 cut(s) 32, 342
Sau3AI GATC 3 cut(s) 102, 163, 269
ScrFI CCNGG 1 cut(s) 154
SetI ASST 7 cut(s) 28, 101, 121, 180, 196, 212, 343
SfcI CTRYAG 1 cut(s) 289
Sse9I AATT 1 cut(s) 256
SspI AATATT 1 cut(s) 12
SspMI CTAG 1 cut(s) 167
StyD4I CCNGG 1 cut(s) 152
TaaI ACNGT 1 cut(s) 146
TaqI TCGA 2 cut(s) 101, 105
TasI AATT 1 cut(s) 256
TatI WGTACW 1 cut(s) 393
Tru1I TTAA 3 cut(s) 8, 219, 255
Tru9I TTAA 3 cut(s) 8, 219, 255
TscAI CASTG 1 cut(s) 149
TseFI GTSAC 1 cut(s) 94
TseI GCWGC 2 cut(s) 31, 341
Tsp45I GTSAC 1 cut(s) 94
TspDTI ATGAA 2 cut(s) 34, 240
TspGWI ACGGA 1 cut(s) 282
TspRI CASTG 1 cut(s) 149
XspI CTAG 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.