Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0005431

Physical Location & Seq

Genomic Coordinates Reverse (-)

21095197 .. 21102612

Gene Structure 7416 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g28770.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2760 bp

ATGCGAAGCAAAAAAGAATCAGCATCCGCCTCAGGATCTCTGGGTGAATTATCAGAGGAGGCTGTGGCCAAACTTGTTAGACGTGCAAATAGAGGTGGGAAAAAGAAATTTGAGAGTCAACTTCATCCAAGTGATTTGATTGGAAAGCATGAGCCCGGGCCACAAAGAGACAAAAAAGATGTGGATGCAAGAGTTGCATCCAATGCCTTGGAGACTGAAGTTTGTAGCAGAGATGCTCTGCGAAAGGTATCTAGGGACACGAATGGTGACGAGGAAAGCTTTCAGTGCTCTTTTATGGATAGCAGGGAAGAACTGAATGACTCAGATTGGGAAGATGGCCCAGTTCCAATTTCAAATTCCATGGGTGGTCATGAAGTGACTATTGAAATAAATGAGACACCAGATTCCAGAAGACGGAAACGTAGTCGTCGGGCTTCGGTTGAAGATAAGGAAGTGGCTGAACTTGTTCATAAGGCTCATTTACTCTGTTTGATTGCACGGGGAAGATTGATTGACAGAGCGTGTGATGATGCTCTTATTCAGGCTTCCTTGCTTTCTCTTCTACCCGAACATTTGCTGCGTGTATCCAAGGTTGCAAAACTTACTGTGAAGCATTTGCTTCCCCTAGTCTTTTGGTTCCAAAATAATTTCCGTGTTAGAACTACAAGTGTGAGGAGATCATTTCATTTGGCTCTGAACTTTGCCCTTGAAACTCGTGAAGGGACTCAAGAAGAGATTGCTGCATTGTCTGTGGCACTGTTCAGAGCTTTGAATCTGACAACTCGGTTGGTGTCCGTTTTGAATGTTGCCTCCTTAAAGCCAGAGGCTGACAAGACTGATTGGTCAAGTGAAGATGCAAGCAGATTGAGTAAGGGAATATTCAGCACTGCAACTCCAATGGTGGCTAGAAAAAATGTTCCTGTGTCTCCTGCTACAAGTAGTGAGAGAAACTCTGTCGGTGAAACTCCTCAGATTGGTTCATATAAATATACTTTAGCTTGTGAGGAATGGAATGATATCTCTGAAGCATGCCATACTAAGAAATCAAAGGAATTAAAGAGAAGAGGGGATCTTGAGTTTGAAATGCAGATGCAAATGGCTCTTTCCGCCACTGCAGTTCCGACTGCGGATATAAAATTAGGTTCAGACAATAATGACTCAGATAGTAATGTTGCAAAAAGATTGAAAAGAACTGTATGTGAAGAATCACAATTTTCCTCCCAGAGTATTTCTACTGCAGTTGGATCGAGAAAAGAAGGATCACCATTGTACTGGGCAGAAGTGTACTGCAATGGGGAAAACTTGACCGGAAAGTGGCTGCATATTGATGCTATTAATGCAATTATTGATGGAGAACAGAAGGTCGAAGCTGTAGCTGCTGCATGCAAAACACCTTTGAGATATGTAGTTGCTTTTGCTGGTAACGGGGCTAAAGACGTGACTCGCAGGTACTGTCTCAAGTGGTATCAGATAGCATCTCAACGAGTAGATCCAATTTGGTGGGATCAGGTATTGGCACCACTGAGAGACTTAGAGGTGAGAGCAACAGGAGGCATGGTCTTTTTGGAGAAAGAACACACCGGTTCCTCATCTGAACATATTATAGAAAATTTTCTGAACATCTCTGGCAGTGCAGAAATGTCCACACCGGTTCCAAGTAATGTCCACTTGAACGCGAAGTCTAGCCTAGAAGGCTCAAAAGATTCTGGAAAGGGGCTTGGCGTAGAATCTTCTTCGAGGAGTGTTGAGATTGCTACCAGGAACTCCCTTGAGGAAATGGAATTGGAAACTAGGTCATTAACTGAACCTCTTCCAACGAATCAACAGGCCTATAAAAACCATCACCTTTATGCTATTGAAAAATGGCTTACCAAGCATCAGGTACTTCATCCGAAAGGGCCAATTTTGGGGTTTTGTTCCGGTCATCCAGTTTACCCTAGGACATGTGTGCAAACTCTGAAGTCAAAACACAAATGGCTTAGGGAGGGGCTGCAAGTTAAACCAAATGAGCATCCTGTCAAGGAGCTGAAACGTTCTATCAAGGTTCAAAAAGTTCTTGAAGACGATGGTATCGTTGGGGGCAATTCAATTGCAACTATAGAACTTTATGGGAAGTGGCAACTTGAACCACTGCATCTGCCTCATGCTATCAATGGAAAAGTACCAAAGAATGATCATGGTAATGTGGAAGTATGGTCTGAGAAGTGCCTTCCACCAGGGACTGTGTATTTGAGGTTACCAAGGGTGTTTTCGGTTGCCAAGAGACTAGAAATTGATTACGCTCCCGCCATGGTTGATTTTGAATTCAAAAACGGTCAATCATATCCTGTCTTTGATGGTATAGTAGTGTGTGCTGAGTTCAAGGATGCAATATTGGAGGCTTATGCAGAGGAACGGGACAGAAGAGAGGCTGTAGAAAAGAAAAAATATGAAATGCAAGCTATTTCACGTTGGTATCAGCTTTTGTCTTCCATTGTAACTCGGCAGAGGATACAAAACCGTTACGGGGAGTCTGCATTTACAGTATCAGCTGAGACTGAAAATGTTAGTAAACTAGATGTAAAGCTTGGTGGTGGTAATGATGAGGAGGCTCTTGGATGCCAGCAAGGCCTACATAAAAATACACTGGATGATCGTTCTAGCATGCTGGAAAACCATGAGCATGTATTCTTGACAGAAAACCAGAGTTTTGATAAAGACAACTTGGTGGTTACAAAGAGATGTCTCTGTGGATTTTCAGTGCAAGTGGAAGAACTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000109 GO:0000111 GO:0000217 GO:0000404 GO:0000405 GO:0000715 GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0003690 GO:0003697 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005739 GO:0005886 GO:0006139 GO:0006259 GO:0006281 GO:0006289 GO:0006294 GO:0006298 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007346 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009987 GO:0010564 GO:0016020 GO:0016043 GO:0022607 GO:0030983 GO:0031974 GO:0031981 GO:0032135 GO:0032991 GO:0033554 GO:0034622 GO:0034641 GO:0034644 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051276 GO:0051716 GO:0051726 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070911 GO:0070914 GO:0071214 GO:0071478 GO:0071482 GO:0071704 GO:0071824 GO:0071840 GO:0071942 GO:0071944 GO:0090304 GO:0097159 GO:0104004 GO:1901360 GO:1901363 GO:1901987 GO:1901990 GO:1990391

KEGG Pathways

Metabolic & Signaling

ko03420 map03420

Pfam Domains

Protein Families

BHD_1 BHD_2 BHD_3 Rad4 Transglut_core

Protein Analysis

920

Amino Acids

102.75

Weight (kDa)

6.63

Isoelectric Point (pI)

45.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rad4	PF03835	309 - 412	3.3e-19	Rad4 transglutaminase-like domain
BHD_1	PF10403	503 - 552	4e-17	Rad4 beta-hairpin domain 1
BHD_2	PF10404	556 - 616	9.7e-12	Rad4 beta-hairpin domain 2
BHD_3	PF10405	623 - 696	1.6e-25	Rad4 beta-hairpin domain 3

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005431)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16630 AT5G16630 AT5G16630
fragaria_vesca	FvH4_1g28770 FvH4_1g28770 FvH4_1g28770 FvH4_1g28770
malus_domestica	MD17G1258300.v1.1
prunus_persica	Prupe.3G161000_v2.0.a1 Prupe.3G161000_v2.0.a1 Prupe.3G161000_v2.0.a1 Prupe.3G161000_v2.0.a1
pyrus_communis	pycom17g26020
rosa_chinensis	RchiOBHm_Chr3g0493161
rosa_laevigata	RLG00000022722 RLG00000022723 RLG00000022724
rosa_multiflora	Rmu_co8401441.1_g000001 Rmu_sc0003290.1_g000009 Rmu_sc0009488.1_g000001
rosa_roxburghii	Rroxscaffold_6G00391760
rosa_rugosa	Rorug03G0259500 Rorug03G0259600 Rorug03G0259700
rosa_samantha	Rh3AG308600 Rh3BG344700 Rh3CG341900 Rh3DG344000
rosa_wichuraiana	Rw3G027140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	841
Acc36I	ACCTGC	1 cut(s)	1437
AccB1I	GGYRCC	1 cut(s)	1516
AccII	CGCG	1 cut(s)	1676
AciI	CCGC	4 cut(s)	27, 1107, 1127, 2286
AclI	AACGTT	1 cut(s)	2032
AclWI	GGATC	6 cut(s)	43, 1077, 1252, 1267, 1484, 1512
AcoI	YGGCCR	1 cut(s)	66
AcsI	RAATTY	4 cut(s)	107, 355, 1609, 2303
AcuI	CTGAAG	3 cut(s)	237, 1044, 1979
AfaI	GTAC	5 cut(s)	1271, 1286, 1451, 1884, 2163
AfiI	CCNNNNNNNGG	5 cut(s)	414, 974, 1314, 1907, 2506
AflIII	ACRYGT	1 cut(s)	1943
AgeI	ACCGGT	2 cut(s)	1580, 1648
AjiI	CACGTC	2 cut(s)	83, 1438
AjnI	CCWGG	2 cut(s)	1757, 2215
AjuI	GAANNNNNNNTTGG	4 cut(s)	340, 372, 2233, 2265
Alw21I	GWGCWC	1 cut(s)	290
Alw26I	GTCTC	9 cut(s)	162, 206, 389, 930, 1460, 1521, 2257, 2528, 2729
AlwI	GGATC	6 cut(s)	43, 1077, 1252, 1267, 1484, 1512
AlwNI	CAGNNNCTG	3 cut(s)	827, 1452, 2222
Ama87I	CYCGRG	1 cut(s)	155
AoxI	GGCC	6 cut(s)	66, 158, 337, 1827, 1898, 2608
ApeKI	GCWGC	6 cut(s)	577, 740, 1318, 1376, 1379, 1990
ApoI	RAATTY	4 cut(s)	107, 355, 1609, 2303
ArsI	GACNNNNNNTTYG	2 cut(s)	2212, 2244
AseI	ATTAAT	1 cut(s)	1335
AsiGI	ACCGGT	2 cut(s)	1580, 1648
Asp700I	GAANNNNTTC	4 cut(s)	279, 465, 1611, 1809
AspA2I	CCTAGG	1 cut(s)	1937
AspS9I	GGNCC	3 cut(s)	158, 338, 1898
AsuC2I	CCSGG	2 cut(s)	156, 157
AsuHPI	GGTGA	6 cut(s)	56, 278, 971, 1254, 1549, 1835
AvaI	CYCGRG	1 cut(s)	155
AvrII	CCTAGG	1 cut(s)	1937
AxyI	CCTNAGG	1 cut(s)	31
BaeI	ACNNNNGTAYC	4 cut(s)	2053, 2086, 2438, 2471
BalI	TGGCCA	1 cut(s)	68
BanI	GGYRCC	1 cut(s)	1516
BanII	GRGCYC	1 cut(s)	156
BauI	CACGAG	1 cut(s)	714
BbsI	GAAGAC	3 cut(s)	418, 2067, 2460
Bbv12I	GWGCWC	1 cut(s)	290
BbvI	GCAGC	6 cut(s)	564, 727, 1305, 1363, 1366, 1977
BccI	CCATC	5 cut(s)	329, 1343, 1848, 2060, 2330
BcgI	CGANNNNNNTGC	2 cut(s)	1227, 1261
BciT130I	CCWGG	2 cut(s)	1759, 2217
BciVI	GTATCC	2 cut(s)	595, 2484
BclI	TGATCA	1 cut(s)	2173
BcnI	CCSGG	2 cut(s)	156, 157
BcoDI	GTCTC	9 cut(s)	162, 206, 389, 930, 1460, 1521, 2257, 2528, 2729
BfmI	CTRYAG	6 cut(s)	1113, 1236, 1371, 2097, 2412, 2756
BfuAI	ACCTGC	1 cut(s)	1437
BfuI	GTATCC	2 cut(s)	595, 2484
BglI	GCCNNNNNGGC	2 cut(s)	1692, 2607
BisI	GCNGC	6 cut(s)	578, 741, 1319, 1377, 1380, 1991
BlnI	CCTAGG	1 cut(s)	1937
BlsI	GCNGC	6 cut(s)	579, 742, 1320, 1378, 1381, 1992
Bme1390I	CCNGG	4 cut(s)	156, 157, 1759, 2217
BmeT110I	CYCGRG	1 cut(s)	155
BmgBI	CACGTC	2 cut(s)	83, 1438
BmgT120I	GGNCC	3 cut(s)	158, 338, 1898
BmiI	GGNNCC	4 cut(s)	638, 1518, 1585, 1653
BmrFI	CCNGG	4 cut(s)	156, 157, 1759, 2217
BmrI	ACTGGG	2 cut(s)	335, 1282
BmuI	ACTGGG	2 cut(s)	335, 1282
BpiI	GAAGAC	3 cut(s)	418, 2067, 2460
BplI	GAGNNNNNCTC	2 cut(s)	935, 967
Bpu10I	CCTNAGC	1 cut(s)	1979
BpuEI	CTTGAG	4 cut(s)	711, 1094, 1442, 1790
BpuMI	CCSGG	2 cut(s)	156, 157
BsaJI	CCNNGG	8 cut(s)	155, 207, 360, 588, 1937, 2216, 2240, 2289
BsaWI	WCCGGW	4 cut(s)	1307, 1580, 1648, 1919
BsaXI	ACNNNNNCTCC	4 cut(s)	877, 907, 1542, 1572
Bsc4I	CCNNNNNNNGG	5 cut(s)	414, 974, 1314, 1907, 2506
Bse118I	RCCGGY	2 cut(s)	1580, 1648
Bse1I	ACTGG	4 cut(s)	341, 1277, 1928, 2631
Bse21I	CCTNAGG	1 cut(s)	31
Bse3DI	GCAATG	1 cut(s)	1297
BseBI	CCWGG	2 cut(s)	1759, 2217
BseDI	CCNNGG	8 cut(s)	155, 207, 360, 588, 1937, 2216, 2240, 2289
BseLI	CCNNNNNNNGG	5 cut(s)	414, 974, 1314, 1907, 2506
BseMI	GCAATG	1 cut(s)	1297
BseMII	CTCAG	8 cut(s)	45, 336, 983, 1173, 1514, 2190, 2346, 2523
BseNI	ACTGG	4 cut(s)	341, 1277, 1928, 2631
BseRI	GAGGAG	5 cut(s)	71, 688, 957, 1753, 2600
BseXI	GCAGC	6 cut(s)	564, 727, 1305, 1363, 1366, 1977
BsgI	GTGCAG	1 cut(s)	1653
Bsh1236I	CGCG	1 cut(s)	1676
BshFI	GGCC	6 cut(s)	68, 160, 339, 1829, 1900, 2610
BshNI	GGYRCC	1 cut(s)	1516
BshTI	ACCGGT	2 cut(s)	1580, 1648
BsiHKAI	GWGCWC	1 cut(s)	290
BsiHKCI	CYCGRG	1 cut(s)	155
BsiSI	CCGG	5 cut(s)	156, 1308, 1581, 1649, 1920
BslFI	GGGAC	4 cut(s)	269, 736, 2233, 2411
BslI	CCNNNNNNNGG	5 cut(s)	414, 974, 1314, 1907, 2506
BsmAI	GTCTC	9 cut(s)	162, 206, 389, 930, 1460, 1521, 2257, 2528, 2729
BsmFI	GGGAC	4 cut(s)	269, 736, 2233, 2411
BsnI	GGCC	6 cut(s)	68, 160, 339, 1829, 1900, 2610
BsoBI	CYCGRG	1 cut(s)	155
Bsp1286I	GDGCHC	2 cut(s)	156, 290
Bsp143I	GATC	9 cut(s)	35, 677, 1069, 1244, 1259, 1489, 1504, 2173, 2632
Bsp19I	CCATGG	2 cut(s)	360, 2289
BspACI	CCGC	4 cut(s)	27, 1107, 1127, 2286
BspANI	GGCC	6 cut(s)	68, 160, 339, 1829, 1900, 2610
BspCNI	CTCAG	8 cut(s)	44, 335, 982, 1172, 1515, 2191, 2347, 2524
BspFNI	CGCG	1 cut(s)	1676
BspHI	TCATGA	1 cut(s)	370
BspLI	GGNNCC	4 cut(s)	638, 1518, 1585, 1653
BspMAI	CTGCAG	2 cut(s)	1117, 1240
BspMI	ACCTGC	1 cut(s)	1437
BspPI	GGATC	6 cut(s)	43, 1077, 1252, 1267, 1484, 1512
BspT107I	GGYRCC	1 cut(s)	1516
BsrDI	GCAATG	1 cut(s)	1297
BsrFI	RCCGGY	2 cut(s)	1580, 1648
BsrI	ACTGG	4 cut(s)	341, 1277, 1928, 2631
BssAI	RCCGGY	2 cut(s)	1580, 1648
BssECI	CCNNGG	8 cut(s)	155, 207, 360, 588, 1937, 2216, 2240, 2289
BssMI	GATC	9 cut(s)	35, 677, 1069, 1244, 1259, 1489, 1504, 2173, 2632
BssSI	CACGAG	1 cut(s)	714
BssT1I	CCWWGG	6 cut(s)	207, 360, 588, 1937, 2240, 2289
Bst2BI	CACGAG	1 cut(s)	714
Bst2UI	CCWGG	2 cut(s)	1759, 2217
Bst4CI	ACNGT	8 cut(s)	607, 759, 1195, 1454, 2224, 2315, 2501, 2524
Bst6I	CTCTTC	5 cut(s)	564, 726, 1057, 1815, 2398
BstAPI	GCANNNNNTGC	2 cut(s)	194, 203
BstC8I	GCNNGC	6 cut(s)	859, 1030, 1384, 2439, 2603, 2645
BstDSI	CCRYGG	2 cut(s)	360, 2289
BstEII	GGTNACC	1 cut(s)	2235
BstFNI	CGCG	1 cut(s)	1676
BstKTI	GATC	9 cut(s)	38, 680, 1072, 1247, 1262, 1492, 1507, 2176, 2635
BstMAI	GTCTC	9 cut(s)	162, 206, 389, 930, 1460, 1521, 2257, 2528, 2729
BstMBI	GATC	9 cut(s)	35, 677, 1069, 1244, 1259, 1489, 1504, 2173, 2632
BstMWI	GCNNNNNNNGC	9 cut(s)	194, 203, 285, 1106, 1337, 1376, 1692, 1873, 2607
BstNI	CCWGG	2 cut(s)	1759, 2217
BstNSI	RCATGY	5 cut(s)	1032, 1386, 1947, 2647, 2666
BstPI	GGTNACC	1 cut(s)	2235
BstSCI	CCNGG	4 cut(s)	154, 155, 1757, 2215
BstSFI	CTRYAG	6 cut(s)	1113, 1236, 1371, 2097, 2412, 2756
BstUI	CGCG	1 cut(s)	1676
BstV1I	GCAGC	6 cut(s)	564, 727, 1305, 1363, 1366, 1977
BstV2I	GAAGAC	3 cut(s)	418, 2067, 2460
BstX2I	RGATCY	3 cut(s)	35, 1069, 1489
BstXI	CCANNNNNNTGG	3 cut(s)	208, 1272, 1500
BstYI	RGATCY	3 cut(s)	35, 1069, 1489
Bsu36I	CCTNAGG	1 cut(s)	31
BsuI	GTATCC	2 cut(s)	595, 2484
BsuRI	GGCC	6 cut(s)	68, 160, 339, 1829, 1900, 2610
BtgI	CCRYGG	2 cut(s)	360, 2289
BtrI	CACGTC	2 cut(s)	83, 1438
BtsI	GCAGTG	4 cut(s)	885, 1110, 1636, 2129
BtsIMutI	CAGTG	9 cut(s)	290, 755, 885, 1110, 1520, 1636, 2129, 2624, 2745
BveI	ACCTGC	1 cut(s)	1437
Cac8I	GCNNGC	6 cut(s)	859, 1030, 1384, 2439, 2603, 2645
CaiI	CAGNNNCTG	3 cut(s)	827, 1452, 2222
CciI	TCATGA	1 cut(s)	370
Cfr10I	RCCGGY	2 cut(s)	1580, 1648
Cfr13I	GGNCC	3 cut(s)	158, 338, 1898
Cfr9I	CCCGGG	1 cut(s)	155
Csp6I	GTAC	5 cut(s)	1270, 1285, 1450, 1883, 2162
CspAI	ACCGGT	2 cut(s)	1580, 1648
CviQI	GTAC	5 cut(s)	1270, 1285, 1450, 1883, 2162
DpnI	GATC	9 cut(s)	37, 679, 1071, 1246, 1261, 1491, 1506, 2175, 2634
DpnII	GATC	9 cut(s)	35, 677, 1069, 1244, 1259, 1489, 1504, 2173, 2632
DrdI	GACNNNNNNGTC	1 cut(s)	841
DseDI	GACNNNNNNGTC	1 cut(s)	841
EaeI	YGGCCR	1 cut(s)	66
Eam1104I	CTCTTC	5 cut(s)	564, 726, 1057, 1815, 2398
EarI	CTCTTC	5 cut(s)	564, 726, 1057, 1815, 2398
EciI	GGCGGA	2 cut(s)	16, 1096
Eco130I	CCWWGG	6 cut(s)	207, 360, 588, 1937, 2240, 2289
Eco147I	AGGCCT	2 cut(s)	1829, 2610
Eco24I	GRGCYC	1 cut(s)	156
Eco32I	GATATC	1 cut(s)	1018
Eco57I	CTGAAG	3 cut(s)	237, 1044, 1979
Eco81I	CCTNAGG	1 cut(s)	31
Eco88I	CYCGRG	1 cut(s)	155
Eco91I	GGTNACC	1 cut(s)	2235
EcoO65I	GGTNACC	1 cut(s)	2235
EcoRI	GAATTC	1 cut(s)	2303
EcoRII	CCWGG	2 cut(s)	1757, 2215
EcoRV	GATATC	1 cut(s)	1018
EcoT14I	CCWWGG	6 cut(s)	207, 360, 588, 1937, 2240, 2289
EcoT38I	GRGCYC	1 cut(s)	156
ErhI	CCWWGG	6 cut(s)	207, 360, 588, 1937, 2240, 2289
FaqI	GGGAC	4 cut(s)	269, 736, 2233, 2411
FauI	CCCGC	1 cut(s)	2293
FbaI	TGATCA	1 cut(s)	2173
Fnu4HI	GCNGC	6 cut(s)	578, 741, 1319, 1377, 1380, 1991
FriOI	GRGCYC	1 cut(s)	156
Fsp4HI	GCNGC	6 cut(s)	578, 741, 1319, 1377, 1380, 1991
GluI	GCNGC	6 cut(s)	578, 741, 1319, 1377, 1380, 1991
HaeIII	GGCC	6 cut(s)	68, 160, 339, 1829, 1900, 2610
HapII	CCGG	5 cut(s)	156, 1308, 1581, 1649, 1920
HincII	GTYRAC	1 cut(s)	119
HindII	GTYRAC	1 cut(s)	119
HindIII	AAGCTT	2 cut(s)	277, 2564
HpaII	CCGG	5 cut(s)	156, 1308, 1581, 1649, 1920
HphI	GGTGA	6 cut(s)	56, 278, 971, 1254, 1549, 1835
Hpy166II	GTNNAC	6 cut(s)	119, 1285, 1644, 1666, 1933, 2552
Hpy8I	GTNNAC	6 cut(s)	119, 1285, 1644, 1666, 1933, 2552
Hpy99I	CGWCG	1 cut(s)	432
HpyAV	CCTTC	5 cut(s)	713, 1250, 1354, 1685, 2219
HpyCH4III	ACNGT	8 cut(s)	607, 759, 1195, 1454, 2224, 2315, 2501, 2524
HpyCH4IV	ACGT	5 cut(s)	82, 421, 1437, 2032, 2449
HpyF10VI	GCNNNNNNNGC	9 cut(s)	194, 203, 285, 1106, 1337, 1376, 1692, 1873, 2607
HpySE526I	ACGT	5 cut(s)	82, 421, 1437, 2032, 2449
Ksp22I	TGATCA	1 cut(s)	2173
Kzo9I	GATC	9 cut(s)	35, 677, 1069, 1244, 1259, 1489, 1504, 2173, 2632
LmnI	GCTCC	2 cut(s)	2023, 2287
Lsp1109I	GCAGC	6 cut(s)	564, 727, 1305, 1363, 1366, 1977
MaeII	ACGT	5 cut(s)	82, 421, 1437, 2032, 2449
MaeIII	GTNAC	8 cut(s)	266, 376, 1421, 1438, 2235, 2476, 2501, 2710
MalI	GATC	9 cut(s)	37, 679, 1071, 1246, 1261, 1491, 1506, 2175, 2634
MboI	GATC	9 cut(s)	35, 677, 1069, 1244, 1259, 1489, 1504, 2173, 2632
MfeI	CAATTG	1 cut(s)	2088
MflI	RGATCY	3 cut(s)	35, 1069, 1489
MhlI	GDGCHC	2 cut(s)	156, 290
MlsI	TGGCCA	1 cut(s)	68
MluNI	TGGCCA	1 cut(s)	68
MlyI	GAGTC	6 cut(s)	124, 314, 718, 1151, 1435, 2519
MmeI	TCCRAC	3 cut(s)	1145, 1222, 1838
Mox20I	TGGCCA	1 cut(s)	68
MroXI	GAANNNNTTC	4 cut(s)	279, 465, 1611, 1809
MscI	TGGCCA	1 cut(s)	68
MseI	TTAA	5 cut(s)	815, 1055, 1335, 1799, 1998
MslI	CAYNNNNRTG	5 cut(s)	129, 1326, 1848, 2181, 2661
Msp20I	TGGCCA	1 cut(s)	68
MspA1I	CMGCKG	1 cut(s)	2531
MspI	CCGG	5 cut(s)	156, 1308, 1581, 1649, 1920
MspR9I	CCNGG	4 cut(s)	156, 157, 1759, 2217
MunI	CAATTG	1 cut(s)	2088
MvaI	CCWGG	2 cut(s)	1759, 2217
MvnI	CGCG	1 cut(s)	1676
MwoI	GCNNNNNNNGC	9 cut(s)	194, 203, 285, 1106, 1337, 1376, 1692, 1873, 2607
NciI	CCSGG	2 cut(s)	156, 157
NcoI	CCATGG	2 cut(s)	360, 2289
NdeII	GATC	9 cut(s)	35, 677, 1069, 1244, 1259, 1489, 1504, 2173, 2632
NlaIV	GGNNCC	4 cut(s)	638, 1518, 1585, 1653
NmeAIII	GCCGAG	1 cut(s)	2461
NmuCI	GTSAC	3 cut(s)	266, 376, 1438
NspI	RCATGY	5 cut(s)	1032, 1386, 1947, 2647, 2666
PaeI	GCATGC	3 cut(s)	1032, 1386, 2647
PagI	TCATGA	1 cut(s)	370
PceI	AGGCCT	2 cut(s)	1829, 2610
PciI	ACATGT	1 cut(s)	1943
PcsI	WCGNNNNNNNCGW	1 cut(s)	2070
PdmI	GAANNNNTTC	4 cut(s)	279, 465, 1611, 1809
PfeI	GAWTC	7 cut(s)	17, 404, 772, 1205, 1703, 1727, 1819
PinAI	ACCGGT	2 cut(s)	1580, 1648
PkrI	GCNGC	6 cut(s)	579, 742, 1320, 1378, 1381, 1992
PleI	GAGTC	6 cut(s)	123, 314, 718, 1151, 1435, 2518
PpsI	GAGTC	6 cut(s)	123, 314, 718, 1151, 1435, 2518
PscI	ACATGT	1 cut(s)	1943
PshBI	ATTAAT	1 cut(s)	1335
Psp1406I	AACGTT	1 cut(s)	2032
Psp6I	CCWGG	2 cut(s)	1757, 2215
PspEI	GGTNACC	1 cut(s)	2235
PspGI	CCWGG	2 cut(s)	1757, 2215
PspN4I	GGNNCC	4 cut(s)	638, 1518, 1585, 1653
PspPI	GGNCC	3 cut(s)	158, 338, 1898
PstI	CTGCAG	2 cut(s)	1117, 1240
PstNI	CAGNNNCTG	3 cut(s)	827, 1452, 2222
PsuI	RGATCY	3 cut(s)	35, 1069, 1489
PvuII	CAGCTG	1 cut(s)	2531
RsaI	GTAC	5 cut(s)	1271, 1286, 1451, 1884, 2163
RsaNI	GTAC	5 cut(s)	1270, 1285, 1450, 1883, 2162
RseI	CAYNNNNRTG	5 cut(s)	129, 1326, 1848, 2181, 2661
SaqAI	TTAA	5 cut(s)	815, 1055, 1335, 1799, 1998
SatI	GCNGC	6 cut(s)	578, 741, 1319, 1377, 1380, 1991
Sau3AI	GATC	9 cut(s)	35, 677, 1069, 1244, 1259, 1489, 1504, 2173, 2632
Sau96I	GGNCC	3 cut(s)	158, 338, 1898
SchI	GAGTC	6 cut(s)	124, 314, 718, 1151, 1435, 2519
ScrFI	CCNGG	4 cut(s)	156, 157, 1759, 2217
SduI	GDGCHC	2 cut(s)	156, 290
SfcI	CTRYAG	6 cut(s)	1113, 1236, 1371, 2097, 2412, 2756
SmaI	CCCGGG	1 cut(s)	157
SmiMI	CAYNNNNRTG	5 cut(s)	129, 1326, 1848, 2181, 2661
SmlI	CTYRAG	4 cut(s)	726, 1073, 1457, 1769
SmoI	CTYRAG	4 cut(s)	726, 1073, 1457, 1769
SphI	GCATGC	3 cut(s)	1032, 1386, 2647
SrfI	GCCCGGGC	1 cut(s)	157
SseBI	AGGCCT	2 cut(s)	1829, 2610
SsiI	CCGC	4 cut(s)	27, 1107, 1127, 2286
SspI	AATATT	2 cut(s)	879, 2373
StuI	AGGCCT	2 cut(s)	1829, 2610
StyD4I	CCNGG	4 cut(s)	154, 155, 1757, 2215
StyI	CCWWGG	6 cut(s)	207, 360, 588, 1937, 2240, 2289
TaaI	ACNGT	8 cut(s)	607, 759, 1195, 1454, 2224, 2315, 2501, 2524
TaiI	ACGT	5 cut(s)	85, 424, 1440, 2035, 2452
TaqI	TCGA	3 cut(s)	1247, 1365, 1736
TatI	WGTACW	2 cut(s)	1269, 1284
TfiI	GAWTC	7 cut(s)	17, 404, 772, 1205, 1703, 1727, 1819
Tru1I	TTAA	5 cut(s)	815, 1055, 1335, 1799, 1998
Tru9I	TTAA	5 cut(s)	815, 1055, 1335, 1799, 1998
TscAI	CASTG	9 cut(s)	290, 762, 892, 1117, 1527, 1636, 2136, 2631, 2745
TseFI	GTSAC	3 cut(s)	266, 376, 1438
TseI	GCWGC	6 cut(s)	577, 740, 1318, 1376, 1379, 1990
Tsp45I	GTSAC	3 cut(s)	266, 376, 1438
TspDTI	ATGAA	7 cut(s)	113, 387, 458, 674, 969, 1877, 2445
TspGWI	ACGGA	3 cut(s)	430, 641, 784
TspMI	CCCGGG	1 cut(s)	155
TspRI	CASTG	9 cut(s)	290, 762, 892, 1117, 1527, 1636, 2136, 2631, 2745
VspI	ATTAAT	1 cut(s)	1335
XapI	RAATTY	4 cut(s)	107, 355, 1609, 2303
XceI	RCATGY	5 cut(s)	1032, 1386, 1947, 2647, 2666
XmaI	CCCGGG	1 cut(s)	155
XmaJI	CCTAGG	1 cut(s)	1937
XmnI	GAANNNNTTC	4 cut(s)	279, 465, 1611, 1809

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.