Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0005431

Physical Location & Seq

Genomic Coordinates Forward (+)

31862463 .. 31869530

Gene Structure 7068 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1258300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2730 bp

ATGCGAAGCGAAAACAAGACGAACCGTTCCAAACAATCGTCATCAGGAACTCTTGCCGACATATCACATGAGGCTGTCAGCAAACTTCTGGGGCGTGCCAATAAAACTGGGAAAAGGAAATCTAACAATAGTCTGCGTCCATGTGATTCAATCGGAAAGCCTGAGAGCGAGCCAAAAAGTGACAAAGATGTCGATTCAAGAGTCAGAGGGAATATTTTGGAATCTGAGGGTTGTAGTAAAGATGCAAATCGAAAGGTCTCTGCAGAAGAGAAAGTTGATGGGGAAGGCTTTGGATGCACTTCCACAAACATTAGGGAAGAACTGGATGACTCAGATTGGGAAGAGGGTTCAATACCCATTTCTAATTCCGCTGGCGATCATGAAGTGACTATTGAATTAAATGAGACACCAGATTCTACAAGAAAGAAACGTATCCGTCGGGCTTCTGCTGAAGATAAGGAACTGGCTGAGCTTGTTCATAGGGTTCATTTACTTTGTTTACTTGCACGGGGAAGGTTGATTGATAGAGCTTGTGATGATGCTCTTATTCAGGCCGCTTTACTTTCTCTGCTTCCACTACACGTGCTGAATACATCCAAGGTTGCAAACCTTACTACAAAGGATTTTAGTCCTCTAGTCGTCTGGTTTCAAAGTAACTTCCATGTTCGAAGTACAAGTGTGAGCAGGCCATTTTCTTCGGGTCTGAAATTTGCCCTTGAAACTCATGAAGGAACTCAAGAAGAGATTGCTGCTTTGTCTGTGGCACTCTTTAGAGCATTAAATCTGACAACGCGGTTCGTCTCGGTTTTGGATGTTGCCTCTTTAAAACCAGAAGCTGGCAAGACCGAATATTCTAGTGAAGATGTAAGCAGATCGAGTAGGAGAATATTCAGTTCTTCGACCCCAATGGTGGCTAGACAAAAGGTCTCTGTCTCTCCTGCCAAATCACTTTCATGTAGTACGAGTGGCAATGTCTATGGAACTTCTCGGGCGTGTTCAAGCGAAAGTAAGGACCCCACAAGAAACAATACTCTGCCCACAAGCTCTGGTATCGCTTATGTGACGAATGATAAATTGTCAGATACTTCTGCAACACAATATGACATCTCTCAAGCATTCGTTAATGAGAAATCTCTGGGATCAAAAAGGAAAGGGGATCATGAGTTCGAAATGCAGTTGCAAATGGCTCTTTCTGCCACAGCAGTGCCGACTGCAGATAGAGAAATGGGTTCAGGTCAGAAAGACTTGAGTGGAAATTTTGCAAATTTCCCTGACCTGAAAAGAATGAAAAGAATTGTTTGTGAAGAATCACAAAACCCCTCCCAGAGTATCTCTACTGCAGTTGGCTCGAGAAAAGAAGGATCTCCTTTGTACTGGGCAGAAGTATACTGTCAGGGGAAGTGGGTGCATATAGATGCTATCAATGGAATTATTGATGGAGAGCAGAAGGTTGAAGCCATAGCTGCTGCATGCAAAACGTCTTTGAGATATGCAGTTGCTTTTGCTGGGAATGGGGCGAAAGATGTGACTCGCAGGTACTGTCTGAAGTGGTATCAGATAGCATCCCAACGAGTCAATTCAATATGGTGGGATGCAGTACTGGCACCCTTGAGAAATTTAGAGGTGAGAGCAACATGTGGCATAGTTAAGTTGGAGAAAGAATGGAATGGTGCGGCCATGGATTATCATCCAACTATGATTGATGTTTCCCGAAATTCTCTAGAGGATATGGAGTTGGAAACTAGGGCCTTAACTGAGCCTCTTCCAACTAATCAGCAGGCCTATAAAAACCATCAACTATATGCTATAGAAAAATGGCTTACAAGGGATCAGGTACTTCATCCGAAGGGCCCTGTTTTGGGTTTTTGTTCTGGTCATCCAGTTTACCCTAGGACATGTGTGCAAACACTAAAGACAAGAGAAAGATGGCTGCGGGAGGGGCTACAAGTTAAAACCAATGAGCATCCTGTGAAGGAGTTGAAACGTTCTCCAAAAGTCCAAAAAGCACAGGTACCTGAATCTGATGATTTTGTTGGGGGTAATTCGAAACGAACTCTTGAACTTTATGGGAAGTGGCAACTTGAACCATTGCATCTGGCTCATGCTGTTAATGGGATAGTGCCAAAGAATGATTACGGTAATGTGGAAGTATGGTCTGAGAAGTGCCTTCCGCCAGGGACTGTGCATTTGAGGTTGCCGAGGCTGTTTTATGTTGCCAAGAGACTGGAAATTGATTATGCTCCCGCCATGGTTGGTTTCGAATATAAAAATGGTCAATCGTTTCCTGTCTTTGATGGTATAGTAGTCTGCTCTGAGTTCAAGGATGCAATATTGGAGGCATATGCAGAGGAAGAGGAAAGAAGAGACGCTGCAGAAAAGAAAAGAAACCAAGGGCAGGCTATTTCTCGGTGGTATCAGCTTCTCTGTTCGATTGTAACTCGACAAAGGTTAAACAACCGCTATGGGGACTCCCCCTCTTCTACAGCATCAGTTGACACCAACAAGGTGAATAGCAACATAGACGTACAGGTTGATGATCGTCAGAATGATAAGCAATCTCTTGCATGCCAGCAAAATGTGCCTGAAAAGAGGACAGCTGATCCATCAGCTTCCATGCCCGAAGATCACGAGCATGTATTCTTGACCGAAAACCAGAGTTTTGATGAGGGAAACTTGGTGCTGACAAAGAGGTGTCAATGTGGATTCTCAGTGCAAGTTGAACAACTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000109 GO:0000111 GO:0000217 GO:0000404 GO:0000405 GO:0000715 GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0003690 GO:0003697 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005739 GO:0005886 GO:0006139 GO:0006259 GO:0006281 GO:0006289 GO:0006294 GO:0006298 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007346 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009987 GO:0010564 GO:0016020 GO:0016043 GO:0022607 GO:0030983 GO:0031974 GO:0031981 GO:0032135 GO:0032991 GO:0033554 GO:0034622 GO:0034641 GO:0034644 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051276 GO:0051716 GO:0051726 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070911 GO:0070914 GO:0071214 GO:0071478 GO:0071482 GO:0071704 GO:0071824 GO:0071840 GO:0071942 GO:0071944 GO:0090304 GO:0097159 GO:0104004 GO:1901360 GO:1901363 GO:1901987 GO:1901990 GO:1990391

KEGG Pathways

Metabolic & Signaling

ko03420 map03420

Pfam Domains

Protein Families

BHD_1 BHD_2 BHD_3 Rad4 Transglut_core

Protein Analysis

910

Amino Acids

101.22

Weight (kDa)

7.24

Isoelectric Point (pI)

47.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rad4	PF03835	439 - 579	1.5e-18	Rad4 transglutaminase-like domain
BHD_1	PF10403	585 - 634	7.9e-17	Rad4 beta-hairpin domain 1
BHD_2	PF10404	638 - 698	1.4e-11	Rad4 beta-hairpin domain 2
BHD_3	PF10405	707 - 780	3.2e-27	Rad4 beta-hairpin domain 3

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005431)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16630 AT5G16630 AT5G16630
fragaria_vesca	FvH4_1g28770 FvH4_1g28770 FvH4_1g28770 FvH4_1g28770
malus_domestica	MD17G1258300.v1.1
prunus_persica	Prupe.3G161000_v2.0.a1 Prupe.3G161000_v2.0.a1 Prupe.3G161000_v2.0.a1 Prupe.3G161000_v2.0.a1
pyrus_communis	pycom17g26020
rosa_chinensis	RchiOBHm_Chr3g0493161
rosa_laevigata	RLG00000022722 RLG00000022723 RLG00000022724
rosa_multiflora	Rmu_co8401441.1_g000001 Rmu_sc0003290.1_g000009 Rmu_sc0009488.1_g000001
rosa_roxburghii	Rroxscaffold_6G00391760
rosa_rugosa	Rorug03G0259500 Rorug03G0259600 Rorug03G0259700
rosa_samantha	Rh3AG308600 Rh3BG344700 Rh3CG341900 Rh3DG344000
rosa_wichuraiana	Rw3G027140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	188
Acc36I	ACCTGC	1 cut(s)	1524
Acc65I	GGTACC	1 cut(s)	2011
AccB1I	GGYRCC	2 cut(s)	1603, 2011
AccB7I	CCANNNNNTGG	1 cut(s)	836
AccI	GTMKAC	1 cut(s)	1386
AccII	CGCG	1 cut(s)	793
AciI	CCGC	8 cut(s)	369, 555, 793, 1673, 1933, 2171, 2244, 2458
AclI	AACGTT	1 cut(s)	1984
AclWI	GGATC	5 cut(s)	1147, 1164, 1369, 1836, 2594
AcoI	YGGCCR	1 cut(s)	1674
AcsI	RAATTY	5 cut(s)	707, 1255, 1264, 1615, 1714
AcuI	CTGAAG	2 cut(s)	471, 1565
AcvI	CACGTG	1 cut(s)	583
AfaI	GTAC	8 cut(s)	673, 961, 1373, 1538, 1599, 1836, 2013, 2526
AfiI	CCNNNNNNNGG	6 cut(s)	836, 910, 1858, 1859, 2395, 2464
AflIII	ACRYGT	3 cut(s)	580, 1634, 1895
AhdI	GACNNNNNGTC	1 cut(s)	923
AjnI	CCWGG	1 cut(s)	2173
AleI	CACNNNNGTG	1 cut(s)	1202
AloI	GAACNNNNNNTCC	2 cut(s)	1148, 1180
AluBI	AGCT	8 cut(s)	472, 530, 836, 1044, 1463, 2419, 2597, 2609
AluI	AGCT	8 cut(s)	472, 530, 836, 1044, 1463, 2419, 2597, 2609
Alw26I	GTCTC	7 cut(s)	262, 398, 805, 931, 937, 2215, 2358
AlwI	GGATC	5 cut(s)	1147, 1164, 1369, 1836, 2594
AlwNI	CAGNNNCTG	3 cut(s)	836, 1539, 2180
Ama87I	CYCGRG	2 cut(s)	987, 1348
AoxI	GGCC	6 cut(s)	552, 686, 1674, 1746, 1779, 1849
ApaI	GGGCCC	1 cut(s)	1853
ApeKI	GCWGC	5 cut(s)	749, 1463, 1466, 1930, 2369
ApoI	RAATTY	5 cut(s)	707, 1255, 1264, 1615, 1714
ArsI	GACNNNNNNTTYG	2 cut(s)	2170, 2202
Asp718I	GGTACC	1 cut(s)	2011
AspA2I	CCTAGG	1 cut(s)	1889
AspS9I	GGNCC	4 cut(s)	1012, 1746, 1849, 1850
AsuHPI	GGTGA	2 cut(s)	1636, 2518
AsuII	TTCGAA	4 cut(s)	667, 1167, 2045, 2259
AvaI	CYCGRG	2 cut(s)	987, 1348
AvaII	GGWCC	1 cut(s)	1012
AvrII	CCTAGG	1 cut(s)	1889
BaeGI	GKGCMC	1 cut(s)	1853
BanI	GGYRCC	2 cut(s)	1603, 2011
BanII	GRGCYC	1 cut(s)	1853
BauI	CACGAG	1 cut(s)	2627
BbrPI	CACGTG	1 cut(s)	583
BbvI	GCAGC	5 cut(s)	736, 1450, 1453, 1917, 2356
BccI	CCATC	6 cut(s)	272, 1430, 1800, 1920, 2288, 2611
BcgI	CGANNNNNNTGC	2 cut(s)	1329, 1363
BciT130I	CCWGG	1 cut(s)	2175
BciVI	GTATCC	1 cut(s)	443
BcoDI	GTCTC	7 cut(s)	262, 398, 805, 931, 937, 2215, 2358
BfaI	CTAG	6 cut(s)	635, 855, 915, 1721, 1743, 1890
BfmI	CTRYAG	7 cut(s)	261, 1212, 1338, 1806, 2370, 2481, 2726
BfuAI	ACCTGC	1 cut(s)	1524
BfuI	GTATCC	1 cut(s)	443
BisI	GCNGC	7 cut(s)	555, 750, 1464, 1467, 1674, 1931, 2370
BlnI	CCTAGG	1 cut(s)	1889
BlpI	GCTNAGC	1 cut(s)	468
BlsI	GCNGC	7 cut(s)	556, 751, 1465, 1468, 1675, 1932, 2371
BmcAI	AGTACT	1 cut(s)	1599
Bme1390I	CCNGG	1 cut(s)	2175
Bme18I	GGWCC	1 cut(s)	1012
BmeRI	GACNNNNNGTC	1 cut(s)	923
BmeT110I	CYCGRG	2 cut(s)	987, 1348
BmgT120I	GGNCC	4 cut(s)	1012, 1746, 1849, 1850
BmiI	GGNNCC	4 cut(s)	1014, 1605, 1851, 2013
BmrFI	CCNGG	1 cut(s)	2175
BmrI	ACTGGG	2 cut(s)	117, 1384
BmuI	ACTGGG	2 cut(s)	117, 1384
Bpu1102I	GCTNAGC	1 cut(s)	468
Bpu14I	TTCGAA	4 cut(s)	667, 1167, 2045, 2259
BpuEI	CTTGAG	4 cut(s)	720, 1095, 1267, 1630
BsaAI	YACGTR	1 cut(s)	583
BsaBI	GATNNNNATC	2 cut(s)	246, 1686
BsaI	GGTCTC	2 cut(s)	262, 931
BsaJI	CCNNGG	7 cut(s)	597, 1677, 1889, 2174, 2198, 2247, 2389
Bsc4I	CCNNNNNNNGG	6 cut(s)	836, 910, 1858, 1859, 2395, 2464
Bse1I	ACTGG	7 cut(s)	112, 327, 468, 1379, 1605, 1880, 2229
Bse3DI	GCAATG	2 cut(s)	976, 2087
Bse8I	GATNNNNATC	2 cut(s)	246, 1686
BseBI	CCWGG	1 cut(s)	2175
BseDI	CCNNGG	7 cut(s)	597, 1677, 1889, 2174, 2198, 2247, 2389
BseJI	GATNNNNATC	2 cut(s)	246, 1686
BseLI	CCNNNNNNNGG	6 cut(s)	836, 910, 1858, 1859, 2395, 2464
BseMI	GCAATG	2 cut(s)	976, 2087
BseMII	CTCAG	8 cut(s)	153, 216, 345, 459, 1746, 2148, 2304, 2721
BseNI	ACTGG	7 cut(s)	112, 327, 468, 1379, 1605, 1880, 2229
BseSI	GKGCMC	1 cut(s)	1853
BseXI	GCAGC	5 cut(s)	736, 1450, 1453, 1917, 2356
BseYI	CCCAGC	1 cut(s)	1505
Bsh1236I	CGCG	1 cut(s)	793
BshFI	GGCC	6 cut(s)	554, 688, 1676, 1748, 1781, 1851
BshNI	GGYRCC	2 cut(s)	1603, 2011
BsiHKCI	CYCGRG	2 cut(s)	987, 1348
BslFI	GGGAC	2 cut(s)	2191, 2480
BslI	CCNNNNNNNGG	6 cut(s)	836, 910, 1858, 1859, 2395, 2464
BsmAI	GTCTC	7 cut(s)	262, 398, 805, 931, 937, 2215, 2358
BsmBI	CGTCTC	2 cut(s)	805, 2358
BsmFI	GGGAC	2 cut(s)	2191, 2480
BsmI	GAATGC	1 cut(s)	1115
BsnI	GGCC	6 cut(s)	554, 688, 1676, 1748, 1781, 1851
Bso31I	GGTCTC	2 cut(s)	262, 931
BsoBI	CYCGRG	2 cut(s)	987, 1348
Bsp119I	TTCGAA	4 cut(s)	667, 1167, 2045, 2259
Bsp120I	GGGCCC	1 cut(s)	1849
Bsp1286I	GDGCHC	1 cut(s)	1853
Bsp143I	GATC	9 cut(s)	376, 872, 1139, 1156, 1361, 1828, 2536, 2599, 2623
Bsp1720I	GCTNAGC	1 cut(s)	468
Bsp19I	CCATGG	2 cut(s)	1677, 2247
BspACI	CCGC	8 cut(s)	369, 555, 793, 1673, 1933, 2171, 2244, 2458
BspANI	GGCC	6 cut(s)	554, 688, 1676, 1748, 1781, 1851
BspCNI	CTCAG	8 cut(s)	154, 217, 344, 460, 1747, 2149, 2305, 2720
BspFNI	CGCG	1 cut(s)	793
BspHI	TCATGA	3 cut(s)	379, 724, 1159
BspLI	GGNNCC	4 cut(s)	1014, 1605, 1851, 2013
BspMAI	CTGCAG	4 cut(s)	265, 1216, 1342, 2374
BspMI	ACCTGC	1 cut(s)	1524
BspPI	GGATC	5 cut(s)	1147, 1164, 1369, 1836, 2594
BspT104I	TTCGAA	4 cut(s)	667, 1167, 2045, 2259
BspT107I	GGYRCC	2 cut(s)	1603, 2011
BspTNI	GGTCTC	2 cut(s)	262, 931
BsrDI	GCAATG	2 cut(s)	976, 2087
BsrI	ACTGG	7 cut(s)	112, 327, 468, 1379, 1605, 1880, 2229
BssECI	CCNNGG	7 cut(s)	597, 1677, 1889, 2174, 2198, 2247, 2389
BssMI	GATC	9 cut(s)	376, 872, 1139, 1156, 1361, 1828, 2536, 2599, 2623
BssNAI	GTATAC	1 cut(s)	1387
BssSI	CACGAG	1 cut(s)	2627
BssT1I	CCWWGG	5 cut(s)	597, 1677, 1889, 2247, 2389
Bst1107I	GTATAC	1 cut(s)	1387
Bst2BI	CACGAG	1 cut(s)	2627
Bst2UI	CCWGG	1 cut(s)	2175
Bst4CI	ACNGT	6 cut(s)	26, 1391, 1541, 2138, 2182, 2727
Bst6I	CTCTTC	7 cut(s)	261, 336, 735, 1767, 2346, 2356, 2482
BstAPI	GCANNNNNTGC	1 cut(s)	2578
BstBAI	YACGTR	1 cut(s)	583
BstBI	TTCGAA	4 cut(s)	667, 1167, 2045, 2259
BstDEI	CTNAG	8 cut(s)	162, 225, 331, 468, 1755, 2157, 2313, 2707
BstDSI	CCRYGG	2 cut(s)	1677, 2247
BstFNI	CGCG	1 cut(s)	793
BstKTI	GATC	9 cut(s)	379, 875, 1142, 1159, 1364, 1831, 2539, 2602, 2626
BstMAI	GTCTC	7 cut(s)	262, 398, 805, 931, 937, 2215, 2358
BstMBI	GATC	9 cut(s)	376, 872, 1139, 1156, 1361, 1828, 2536, 2599, 2623
BstMWI	GCNNNNNNNGC	6 cut(s)	294, 1193, 1463, 1601, 1939, 2578
BstNI	CCWGG	1 cut(s)	2175
BstNSI	RCATGY	5 cut(s)	1473, 1638, 1899, 2568, 2636
BstSCI	CCNGG	1 cut(s)	2173
BstSFI	CTRYAG	7 cut(s)	261, 1212, 1338, 1806, 2370, 2481, 2726
BstSLI	GKGCMC	1 cut(s)	1853
BstUI	CGCG	1 cut(s)	793
BstV1I	GCAGC	5 cut(s)	736, 1450, 1453, 1917, 2356
BstX2I	RGATCY	1 cut(s)	1361
BstXI	CCANNNNNNTGG	1 cut(s)	2224
BstYI	RGATCY	1 cut(s)	1361
BstZ17I	GTATAC	1 cut(s)	1387
BsuI	GTATCC	1 cut(s)	443
BsuRI	GGCC	6 cut(s)	554, 688, 1676, 1748, 1781, 1851
BtgI	CCRYGG	2 cut(s)	1677, 2247
BtsI	GCAGTG	1 cut(s)	1209
BtsIMutI	CAGTG	2 cut(s)	1209, 2715
BveI	ACCTGC	1 cut(s)	1524
CaiI	CAGNNNCTG	3 cut(s)	836, 1539, 2180
CciI	TCATGA	3 cut(s)	379, 724, 1159
Cfr13I	GGNCC	4 cut(s)	1012, 1746, 1849, 1850
CseI	GACGC	2 cut(s)	125, 2375
Csp6I	GTAC	8 cut(s)	672, 960, 1372, 1537, 1598, 1835, 2012, 2525
CviQI	GTAC	8 cut(s)	672, 960, 1372, 1537, 1598, 1835, 2012, 2525
DdeI	CTNAG	8 cut(s)	162, 225, 331, 468, 1755, 2157, 2313, 2707
DpnI	GATC	9 cut(s)	378, 874, 1141, 1158, 1363, 1830, 2538, 2601, 2625
DpnII	GATC	9 cut(s)	376, 872, 1139, 1156, 1361, 1828, 2536, 2599, 2623
DraI	TTTAAA	1 cut(s)	825
DrdI	GACNNNNNNGTC	1 cut(s)	188
DriI	GACNNNNNGTC	1 cut(s)	923
DseDI	GACNNNNNNGTC	1 cut(s)	188
EaeI	YGGCCR	1 cut(s)	1674
Eam1104I	CTCTTC	7 cut(s)	261, 336, 735, 1767, 2346, 2356, 2482
Eam1105I	GACNNNNNGTC	1 cut(s)	923
EarI	CTCTTC	7 cut(s)	261, 336, 735, 1767, 2346, 2356, 2482
EciI	GGCGGA	1 cut(s)	2160
Eco130I	CCWWGG	5 cut(s)	597, 1677, 1889, 2247, 2389
Eco147I	AGGCCT	1 cut(s)	1781
Eco24I	GRGCYC	1 cut(s)	1853
Eco31I	GGTCTC	2 cut(s)	262, 931
Eco47I	GGWCC	1 cut(s)	1012
Eco57I	CTGAAG	2 cut(s)	471, 1565
Eco72I	CACGTG	1 cut(s)	583
Eco88I	CYCGRG	2 cut(s)	987, 1348
EcoO109I	RGGNCCY	4 cut(s)	1012, 1746, 1849, 1850
EcoRII	CCWGG	1 cut(s)	2173
EcoT14I	CCWWGG	5 cut(s)	597, 1677, 1889, 2247, 2389
EcoT38I	GRGCYC	1 cut(s)	1853
ErhI	CCWWGG	5 cut(s)	597, 1677, 1889, 2247, 2389
Esp3I	CGTCTC	2 cut(s)	805, 2358
FaqI	GGGAC	2 cut(s)	2191, 2480
FauI	CCCGC	2 cut(s)	1926, 2251
FauNDI	CATATG	1 cut(s)	2341
FblI	GTMKAC	1 cut(s)	1386
Fnu4HI	GCNGC	7 cut(s)	555, 750, 1464, 1467, 1674, 1931, 2370
FriOI	GRGCYC	1 cut(s)	1853
Fsp4HI	GCNGC	7 cut(s)	555, 750, 1464, 1467, 1674, 1931, 2370
FspBI	CTAG	6 cut(s)	635, 855, 915, 1721, 1743, 1890
GluI	GCNGC	7 cut(s)	555, 750, 1464, 1467, 1674, 1931, 2370
GsaI	CCCAGC	1 cut(s)	1509
HaeIII	GGCC	6 cut(s)	554, 688, 1676, 1748, 1781, 1851
HgaI	GACGC	2 cut(s)	125, 2375
HincII	GTYRAC	1 cut(s)	2494
HindII	GTYRAC	1 cut(s)	2494
HphI	GGTGA	2 cut(s)	1636, 2518
Hpy166II	GTNNAC	4 cut(s)	500, 1387, 1885, 2494
Hpy8I	GTNNAC	4 cut(s)	500, 1387, 1885, 2494
Hpy99I	CGWCG	1 cut(s)	441
HpyAV	CCTTC	8 cut(s)	278, 507, 722, 1352, 1441, 1840, 1966, 2177
HpyCH4III	ACNGT	6 cut(s)	26, 1391, 1541, 2138, 2182, 2727
HpyCH4IV	ACGT	5 cut(s)	430, 582, 1478, 1984, 2523
HpyF10VI	GCNNNNNNNGC	6 cut(s)	294, 1193, 1463, 1601, 1939, 2578
HpyF3I	CTNAG	8 cut(s)	162, 225, 331, 468, 1755, 2157, 2313, 2707
HpySE526I	ACGT	5 cut(s)	430, 582, 1478, 1984, 2523
KpnI	GGTACC	1 cut(s)	2015
Kzo9I	GATC	9 cut(s)	376, 872, 1139, 1156, 1361, 1828, 2536, 2599, 2623
LmnI	GCTCC	1 cut(s)	2245
Lsp1109I	GCAGC	5 cut(s)	736, 1450, 1453, 1917, 2356
MaeI	CTAG	6 cut(s)	635, 855, 915, 1721, 1743, 1890
MaeII	ACGT	5 cut(s)	430, 582, 1478, 1984, 2523
MaeIII	GTNAC	6 cut(s)	179, 385, 653, 1060, 1525, 2434
MalI	GATC	9 cut(s)	378, 874, 1141, 1158, 1363, 1830, 2538, 2601, 2625
MboI	GATC	9 cut(s)	376, 872, 1139, 1156, 1361, 1828, 2536, 2599, 2623
MflI	RGATCY	1 cut(s)	1361
MhlI	GDGCHC	1 cut(s)	1853
MlyI	GAGTC	5 cut(s)	210, 323, 1522, 1581, 2462
MmeI	TCCRAC	4 cut(s)	1632, 1715, 1716, 1790
MseI	TTAA	9 cut(s)	398, 779, 824, 1122, 1647, 1751, 1950, 2109, 2450
MslI	CAYNNNNRTG	4 cut(s)	952, 1202, 1413, 2631
MspA1I	CMGCKG	2 cut(s)	371, 2597
MspR9I	CCNGG	1 cut(s)	2175
Mva1269I	GAATGC	1 cut(s)	1115
MvaI	CCWGG	1 cut(s)	2175
MvnI	CGCG	1 cut(s)	793
MwoI	GCNNNNNNNGC	6 cut(s)	294, 1193, 1463, 1601, 1939, 2578
NcoI	CCATGG	2 cut(s)	1677, 2247
NdeI	CATATG	1 cut(s)	2341
NdeII	GATC	9 cut(s)	376, 872, 1139, 1156, 1361, 1828, 2536, 2599, 2623
NlaIV	GGNNCC	4 cut(s)	1014, 1605, 1851, 2013
NmeAIII	GCCGAG	1 cut(s)	2223
NmuCI	GTSAC	4 cut(s)	179, 385, 1060, 1525
NspI	RCATGY	5 cut(s)	1473, 1638, 1899, 2568, 2636
NspV	TTCGAA	4 cut(s)	667, 1167, 2045, 2259
OliI	CACNNNNGTG	1 cut(s)	1202
PaeI	GCATGC	2 cut(s)	1473, 2568
PaeR7I	CTCGAG	1 cut(s)	1348
PagI	TCATGA	3 cut(s)	379, 724, 1159
PceI	AGGCCT	1 cut(s)	1781
PciI	ACATGT	2 cut(s)	1634, 1895
PctI	GAATGC	1 cut(s)	1115
PfeI	GAWTC	7 cut(s)	146, 194, 221, 413, 1307, 2018, 2703
PflMI	CCANNNNNTGG	1 cut(s)	836
PkrI	GCNGC	7 cut(s)	556, 751, 1465, 1468, 1675, 1932, 2371
PleI	GAGTC	5 cut(s)	209, 323, 1522, 1580, 2462
PmaCI	CACGTG	1 cut(s)	583
PmlI	CACGTG	1 cut(s)	583
PpsI	GAGTC	5 cut(s)	209, 323, 1522, 1580, 2462
Ppu21I	YACGTR	1 cut(s)	583
PpuMI	RGGWCCY	1 cut(s)	1012
PscI	ACATGT	2 cut(s)	1634, 1895
Psp1406I	AACGTT	1 cut(s)	1984
Psp5II	RGGWCCY	1 cut(s)	1012
Psp6I	CCWGG	1 cut(s)	2173
PspCI	CACGTG	1 cut(s)	583
PspFI	CCCAGC	1 cut(s)	1505
PspGI	CCWGG	1 cut(s)	2173
PspN4I	GGNNCC	4 cut(s)	1014, 1605, 1851, 2013
PspOMI	GGGCCC	1 cut(s)	1849
PspPI	GGNCC	4 cut(s)	1012, 1746, 1849, 1850
PspPPI	RGGWCCY	1 cut(s)	1012
PstI	CTGCAG	4 cut(s)	265, 1216, 1342, 2374
PstNI	CAGNNNCTG	3 cut(s)	836, 1539, 2180
PsuI	RGATCY	1 cut(s)	1361
PvuII	CAGCTG	1 cut(s)	2597
RsaI	GTAC	8 cut(s)	673, 961, 1373, 1538, 1599, 1836, 2013, 2526
RsaNI	GTAC	8 cut(s)	672, 960, 1372, 1537, 1598, 1835, 2012, 2525
RseI	CAYNNNNRTG	4 cut(s)	952, 1202, 1413, 2631
SaqAI	TTAA	9 cut(s)	398, 779, 824, 1122, 1647, 1751, 1950, 2109, 2450
SatI	GCNGC	7 cut(s)	555, 750, 1464, 1467, 1674, 1931, 2370
Sau3AI	GATC	9 cut(s)	376, 872, 1139, 1156, 1361, 1828, 2536, 2599, 2623
Sau96I	GGNCC	4 cut(s)	1012, 1746, 1849, 1850
ScaI	AGTACT	1 cut(s)	1599
SchI	GAGTC	5 cut(s)	210, 323, 1522, 1581, 2462
ScrFI	CCNGG	1 cut(s)	2175
SduI	GDGCHC	1 cut(s)	1853
SfcI	CTRYAG	7 cut(s)	261, 1212, 1338, 1806, 2370, 2481, 2726
Sfr274I	CTCGAG	1 cut(s)	1348
SfuI	TTCGAA	4 cut(s)	667, 1167, 2045, 2259
SinI	GGWCC	1 cut(s)	1012
SlaI	CTCGAG	1 cut(s)	1348
SmiMI	CAYNNNNRTG	4 cut(s)	952, 1202, 1413, 2631
SmlI	CTYRAG	5 cut(s)	735, 1110, 1246, 1348, 1609
SmoI	CTYRAG	5 cut(s)	735, 1110, 1246, 1348, 1609
SphI	GCATGC	2 cut(s)	1473, 2568
SseBI	AGGCCT	1 cut(s)	1781
SsiI	CCGC	8 cut(s)	369, 555, 793, 1673, 1933, 2171, 2244, 2458
SspI	AATATT	4 cut(s)	214, 851, 888, 2331
SspMI	CTAG	6 cut(s)	635, 855, 915, 1721, 1743, 1890
StuI	AGGCCT	1 cut(s)	1781
StyD4I	CCNGG	1 cut(s)	2173
StyI	CCWWGG	5 cut(s)	597, 1677, 1889, 2247, 2389
TaaI	ACNGT	6 cut(s)	26, 1391, 1541, 2138, 2182, 2727
TaiI	ACGT	5 cut(s)	433, 585, 1481, 1987, 2526
TaqII	GACCGA	2 cut(s)	860, 2660
TatI	WGTACW	3 cut(s)	671, 1371, 1597
TauI	GCSGC	2 cut(s)	557, 1676
TfiI	GAWTC	7 cut(s)	146, 194, 221, 413, 1307, 2018, 2703
Tru1I	TTAA	9 cut(s)	398, 779, 824, 1122, 1647, 1751, 1950, 2109, 2450
Tru9I	TTAA	9 cut(s)	398, 779, 824, 1122, 1647, 1751, 1950, 2109, 2450
TscAI	CASTG	2 cut(s)	1209, 2715
TseFI	GTSAC	4 cut(s)	179, 385, 1060, 1525
TseI	GCWGC	5 cut(s)	749, 1463, 1466, 1930, 2369
Tsp45I	GTSAC	4 cut(s)	179, 385, 1060, 1525
TspDTI	ATGAA	7 cut(s)	396, 467, 476, 741, 942, 1301, 1829
TspGWI	ACGGA	1 cut(s)	425
TspRI	CASTG	2 cut(s)	1209, 2715
Van91I	CCANNNNNTGG	1 cut(s)	836
VpaK11BI	GGWCC	1 cut(s)	1012
XapI	RAATTY	5 cut(s)	707, 1255, 1264, 1615, 1714
XbaI	TCTAGA	1 cut(s)	1720
XceI	RCATGY	5 cut(s)	1473, 1638, 1899, 2568, 2636
XhoI	CTCGAG	1 cut(s)	1348
XmaJI	CCTAGG	1 cut(s)	1889
XmiI	GTMKAC	1 cut(s)	1386
XspI	CTAG	6 cut(s)	635, 855, 915, 1721, 1743, 1890
ZrmI	AGTACT	1 cut(s)	1599

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.