Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3D

Orthogroup ID

OG0005431

Physical Location & Seq

Genomic Coordinates Reverse (-)

38674957 .. 38684188

Gene Structure 9232 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3DG344000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2904 bp

ATGCGAGGCCAAAAGGAGAGCAAGCGTTCCAAAGAATCAGCATCATCGGCATCAGGAACTCTGGGTGACATATCAGAGGAGGCTGTGGCCAAACTTGTCAGACGTGCAAATAGAAGTGGGAAAAAGAAATGTGAGAATCAACTTCATCCATGTGATTTGATTGGAAAGCAAGAGTCCGGGCCACAAAGAGACAAAAAAGATGTGGATGCAACTGTCGGATCCTATGCCTTGGAGACTCAAGCTTGTAGCAGAGATGCTTTGCAAAAGGTATCTAGGGATGCGAAAGGTGATGGGGAAAGCTTTCAGTGCTCTTTTACAAATAGCAGGGAAGAACTGAATGATTCAGATTGGGAAGATGGCCCAGTTCCAATTTCAAATTCCATGGGTGGTCATGAAGTGACTATTGAAATGAATGAAACACCTGATTCCAGAAGACGGAAACGTAGTCGTCGGGCTTCAGTTGAAGATAAGGAATTGGCTGAACTTGTTCATAAAGCTCATTTACTCTGTTTAATTGCACGGGGAAGATTGATTGACAGAGCTTGTGATGATGCTCTTATTCAGGCTTCCTTACTTTCTGTTCTACCAGAACACTTGCTGCATATATCCAAGGTTGCAAAACTAACTGCGAAGCATTTGCTTCCCCTAGTCTTTTGGTTTCAAAATAATTTCCATGTTAGAACTACAAGCGTGAGAAGATCATTTCATTCGGCTCTGAACTTTGCCCTTGAAACTCATGAAGGGACCCAAGAAGAGATTGCTGCATTGTCTGTGGCACTCTTCAGAGCTTTGAATCTTACAACCCGTTACCATGTTTTGCATATATTTATTTTCAGGTTGGTGTCCATTTTGAATGTTGCCTCCTTAAAACCAGAGGCTGACAAGACTGACTGTTCTAGTGAAGATGCAAGCAGATTGAGTAAGGGAATATTCAGCACTTCAACCCCAATGGTGGCTAGAAGAAATGAGGTTCCTGTGTCTCCTGCTACAAGTAGTGAGAGAAACTCTGTCGGTGAAACTCCTCAGATCGGTTCATACAAAAGTGAGGATTGCCACCTTACCAGTAGCAAAACTCGGTGCAAAGGTTCTTATGTTTATGAGTTGAATGATAAAATGACAGATACTTTCGCATGTGAGGAATGGAATGATATGTCTGAAGCATGCCATACTAAGAAATCACAGGAATCAAAGAGAAGAGGGGATCTTGAGTTTGAAATGCAGTTGCAAATGGCTCTTGCCGCCACGGCAGTTCCGACTGCGGGTATAAAAATAGGTTCAGATAATAAAGACTCAAATAGTAATGTTGCAAAAAGATTGAAAAGAACTGTTTGTGAAGAATCACCAATTTCCTCCCAGAGTATCTCTACTGCAGTTGGATCGAGAAAAGAAGGATCCCCATTGTACTGGGCAGAAGTGTACTGCAATGGGGAAAACTTGACTGGAAAGTGGCTACATATTGATGCTATCAATGCAATTATTGATGGAGAACAGAAGGTCGAAGCTGTAGCTGCTGCATGCAAAACGCCTTTGAGATATGTAGTTGCTTTCGCTGGGAATGGGGCTAAAGATGTGACTCGCAGGTACTGTCTCAAGTGGTATCAGATAGCATCTCAACGAGTGAATCCAATTTGGTGGGATAAGGTATTGGCACCACTGAGAGATTTAGAGGTGAGAGCAACAGGAGGCATGGTCCATTTGGATAAAGAACACACTGGTTCCTCATCTGAGCATAATAAAGAAAATTTTTTGACCAAGTCTGGCAGTGCGGAAATGAATCATCTTCCAGGCAATGTCAACTTGAATGCGAAGTCTAGCCTAGAAGGCTCAAAAGAAACTGGAAAGGGGCTTGATGTAGAATCTTCTTCGAGGAGTTTTGAGATTGCTACCAGGAACTCCCTTGAGGATATGGAGTTGGAAACTAGGGCATTAACTGAGCCTCTTCCAACAAATCAACAGGCCTATAAAAATCATCACCTTTATGCTATTGAAAAATGGCTTACCAAGCATCAGGTACTTCATCCGAAAGGGCCAATTCTGGGGTTTTGTTCCGGTCATCCAGTTTACCCTAGGACATGTGTGCAAACTCTAAAGACAAAACACAAATGGCTGAGGGAGGGGCTGCAAGTTAAACCAAATGAGCGTCCTGTCAAGGAGTTGAAACGTTCTATCAAGCTTCAAAAGGTACAGGTTCTTGAAAATGATGATTATGTCGGGGGTAATTCAATTGGAACTATCGAACTTTATGGGAAGTGGCAACTTGAACCACTGCATCTGCCTCATGCTATTAATGGAAAAGTGCCAAAGAATGATCATGGTAACATGGAAGTATGGTCTGAGAAGTGCCTTCCACCAGGGACTGTGCATTTGAGGTTACCAAGGGTGTTTTCGGTTGCCAAGAGACTAGAAATTGATTACGCTCCCGCCATGGTTGATTTTGAATTCAAAAATGGTCAATCATATCCTGTCTTTGATGGTATAGTGGTGTGTGCTGAGTTCAAGGATGCAATATTGGAGGCTTATGCAGAGGAACGGGACAGAAGAGAGGCTGTAGAAAAGAAAAGATATGAAATGCAAGCTATTTCACGGTGGTATCAGCTTTTGTCTTCCATTGTAACTCGGCAGAGGATACAAAACCGTTATGGGGAGTCTTCATCTACAGCATCAGCTGAGACTGAAAATGTGAATAGTAAACTAGATGTACAGCTTGGTGGTGGTCAGAATGATGAGGAACCTCTTGGATGCCAGCAAGGAGGCCTACATGAAAATACACTGGATGATCCTACCATCGAGCTGTACAATCATGAGCATGTATTCTTGACAGAAAACCAGAGTTTTGATGAAGACAACTTGGTAGTTACAAAGAGGTGTCTCTGTGGATTTTCAGTGCAAGTGGAAGAACTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000109 GO:0000111 GO:0000217 GO:0000404 GO:0000405 GO:0000715 GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0003690 GO:0003697 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005739 GO:0005886 GO:0006139 GO:0006259 GO:0006281 GO:0006289 GO:0006294 GO:0006298 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007346 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009987 GO:0010564 GO:0016020 GO:0016043 GO:0022607 GO:0030983 GO:0031974 GO:0031981 GO:0032135 GO:0032991 GO:0033554 GO:0034622 GO:0034641 GO:0034644 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051276 GO:0051716 GO:0051726 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070911 GO:0070914 GO:0071214 GO:0071478 GO:0071482 GO:0071704 GO:0071824 GO:0071840 GO:0071942 GO:0071944 GO:0090304 GO:0097159 GO:0104004 GO:1901360 GO:1901363 GO:1901987 GO:1901990 GO:1990391

KEGG Pathways

Metabolic & Signaling

ko03420 map03420

Pfam Domains

Protein Families

BHD_1 BHD_2 BHD_3 Rad4 Transglut_core

Protein Analysis

967

Amino Acids

108.19

Weight (kDa)

6.64

Isoelectric Point (pI)

48.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rad4	PF03835	451 - 554	2.5e-19	Rad4 transglutaminase-like domain
BHD_1	PF10403	644 - 693	4.9e-17	Rad4 beta-hairpin domain 1
BHD_2	PF10404	697 - 759	3.3e-12	Rad4 beta-hairpin domain 2
BHD_3	PF10405	766 - 839	6.7e-26	Rad4 beta-hairpin domain 3

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005431)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16630 AT5G16630 AT5G16630
fragaria_vesca	FvH4_1g28770 FvH4_1g28770 FvH4_1g28770 FvH4_1g28770
malus_domestica	MD17G1258300.v1.1
prunus_persica	Prupe.3G161000_v2.0.a1 Prupe.3G161000_v2.0.a1 Prupe.3G161000_v2.0.a1 Prupe.3G161000_v2.0.a1
pyrus_communis	pycom17g26020
rosa_chinensis	RchiOBHm_Chr3g0493161
rosa_laevigata	RLG00000022722 RLG00000022723 RLG00000022724
rosa_multiflora	Rmu_co8401441.1_g000001 Rmu_sc0003290.1_g000009 Rmu_sc0009488.1_g000001
rosa_roxburghii	Rroxscaffold_6G00391760
rosa_rugosa	Rorug03G0259500 Rorug03G0259600 Rorug03G0259700
rosa_samantha	Rh3AG308600 Rh3BG344700 Rh3CG341900 Rh3DG344000
rosa_wichuraiana	Rw3G027140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1569
AccB1I	GGYRCC	1 cut(s)	1648
AciI	CCGC	4 cut(s)	1239, 1259, 1766, 2421
AclI	AACGTT	1 cut(s)	2161
AclWI	GGATC	7 cut(s)	213, 226, 1209, 1384, 1386, 1399, 2771
AcoI	YGGCCR	1 cut(s)	87
AcsI	RAATTY	3 cut(s)	376, 1741, 2438
AcuI	CTGAAG	3 cut(s)	441, 766, 1176
AfaI	GTAC	7 cut(s)	1403, 1418, 1583, 2013, 2184, 2700, 2795
AfiI	CCNNNNNNNGG	6 cut(s)	435, 952, 1028, 1259, 2036, 2641
AflIII	ACRYGT	1 cut(s)	2072
AjiI	CACGTC	1 cut(s)	104
AjnI	CCWGG	3 cut(s)	1783, 1886, 2350
AjuI	GAANNNNNNNTTGG	4 cut(s)	361, 393, 2368, 2400
Alw21I	GWGCWC	1 cut(s)	311
Alw26I	GTCTC	7 cut(s)	183, 227, 984, 1592, 2392, 2663, 2873
AlwI	GGATC	7 cut(s)	213, 226, 1209, 1384, 1386, 1399, 2771
AlwNI	CAGNNNCTG	3 cut(s)	878, 1584, 2357
AoxI	GGCC	7 cut(s)	7, 87, 179, 358, 1956, 2027, 2752
ApeKI	GCWGC	5 cut(s)	598, 761, 1508, 1511, 2119
ApoI	RAATTY	3 cut(s)	376, 1741, 2438
ArsI	GACNNNNNNTTYG	2 cut(s)	2347, 2379
AseI	ATTAAT	1 cut(s)	2286
Asp700I	GAANNNNTTC	2 cut(s)	300, 486
AspA2I	CCTAGG	1 cut(s)	2066
AspS9I	GGNCC	5 cut(s)	179, 359, 744, 1690, 2027
AsuC2I	CCSGG	1 cut(s)	178
AsuHPI	GGTGA	6 cut(s)	77, 299, 1025, 1332, 1681, 1964
AvaII	GGWCC	2 cut(s)	744, 1690
AvrII	CCTAGG	1 cut(s)	2066
BaeI	ACNNNNGTAYC	2 cut(s)	2573, 2606
BalI	TGGCCA	1 cut(s)	89
BamHI	GGATCC	2 cut(s)	218, 1391
BanI	GGYRCC	1 cut(s)	1648
BbsI	GAAGAC	4 cut(s)	439, 2595, 2640, 2847
Bbv12I	GWGCWC	1 cut(s)	311
BbvCI	CCTCAGC	1 cut(s)	2108
BbvI	GCAGC	5 cut(s)	585, 748, 1495, 1498, 2106
BccI	CCATC	5 cut(s)	284, 350, 1475, 2465, 2792
BceAI	ACGGC	1 cut(s)	1260
BcgI	CGANNNNNNTGC	4 cut(s)	619, 653, 1359, 1393
BciT130I	CCWGG	3 cut(s)	1785, 1888, 2352
BciVI	GTATCC	1 cut(s)	2619
BclI	TGATCA	1 cut(s)	2308
BcnI	CCSGG	1 cut(s)	178
BcoDI	GTCTC	7 cut(s)	183, 227, 984, 1592, 2392, 2663, 2873
BfmI	CTRYAG	5 cut(s)	1368, 1503, 2547, 2655, 2900
BfuAI	ACCTGC	1 cut(s)	1569
BfuI	GTATCC	1 cut(s)	2619
BglI	GCCNNNNNGGC	2 cut(s)	1244, 1821
BisI	GCNGC	6 cut(s)	599, 762, 1239, 1509, 1512, 2120
BlnI	CCTAGG	1 cut(s)	2066
BlsI	GCNGC	6 cut(s)	600, 763, 1240, 1510, 1513, 2121
Bme1390I	CCNGG	4 cut(s)	178, 1785, 1888, 2352
Bme18I	GGWCC	2 cut(s)	744, 1690
BmgBI	CACGTC	1 cut(s)	104
BmgT120I	GGNCC	5 cut(s)	179, 359, 744, 1690, 2027
BmiI	GGNNCC	8 cut(s)	220, 745, 746, 972, 1393, 1650, 1717, 2730
BmrFI	CCNGG	4 cut(s)	178, 1785, 1888, 2352
BmrI	ACTGGG	2 cut(s)	356, 1414
BmuI	ACTGGG	2 cut(s)	356, 1414
BpiI	GAAGAC	4 cut(s)	439, 2595, 2640, 2847
BplI	GAGNNNNNCTC	2 cut(s)	989, 1021
Bpu10I	CCTNAGC	1 cut(s)	2108
BpuEI	CTTGAG	4 cut(s)	222, 1226, 1574, 1919
BpuMI	CCSGG	1 cut(s)	178
BsaJI	CCNNGG	8 cut(s)	228, 381, 609, 1242, 2066, 2351, 2375, 2424
BsaWI	WCCGGW	1 cut(s)	2048
BsaXI	ACNNNNNCTCC	2 cut(s)	1674, 1704
Bsc4I	CCNNNNNNNGG	6 cut(s)	435, 952, 1028, 1259, 2036, 2641
Bse1I	ACTGG	8 cut(s)	362, 1062, 1409, 1444, 1717, 1840, 2057, 2775
Bse3DI	GCAATG	2 cut(s)	1429, 1795
BseBI	CCWGG	3 cut(s)	1785, 1888, 2352
BseDI	CCNNGG	8 cut(s)	228, 381, 609, 1242, 2066, 2351, 2375, 2424
BseGI	GGATG	8 cut(s)	145, 211, 283, 2017, 2053, 2506, 2744, 2779
BseLI	CCNNNNNNNGG	6 cut(s)	435, 952, 1028, 1259, 2036, 2641
BseMI	GCAATG	2 cut(s)	1429, 1795
BseMII	CTCAG	8 cut(s)	1037, 1646, 1716, 1923, 2099, 2325, 2481, 2658
BseNI	ACTGG	8 cut(s)	362, 1062, 1409, 1444, 1717, 1840, 2057, 2775
BseRI	GAGGAG	3 cut(s)	92, 1011, 1882
BseXI	GCAGC	5 cut(s)	585, 748, 1495, 1498, 2106
BseYI	CCCAGC	1 cut(s)	1550
BshFI	GGCC	7 cut(s)	9, 89, 181, 360, 1958, 2029, 2754
BshNI	GGYRCC	1 cut(s)	1648
BsiHKAI	GWGCWC	1 cut(s)	311
BsiSI	CCGG	2 cut(s)	177, 2049
BslFI	GGGAC	3 cut(s)	757, 2368, 2546
BslI	CCNNNNNNNGG	6 cut(s)	435, 952, 1028, 1259, 2036, 2641
BsmAI	GTCTC	7 cut(s)	183, 227, 984, 1592, 2392, 2663, 2873
BsmFI	GGGAC	3 cut(s)	757, 2368, 2546
BsmI	GAATGC	1 cut(s)	1807
BsnI	GGCC	7 cut(s)	9, 89, 181, 360, 1958, 2029, 2754
Bsp1286I	GDGCHC	1 cut(s)	311
Bsp1407I	TGTACA	2 cut(s)	2698, 2793
Bsp143I	GATC	8 cut(s)	218, 698, 1026, 1201, 1376, 1391, 2308, 2776
Bsp19I	CCATGG	2 cut(s)	381, 2424
BspACI	CCGC	4 cut(s)	1239, 1259, 1766, 2421
BspANI	GGCC	7 cut(s)	9, 89, 181, 360, 1958, 2029, 2754
BspCNI	CTCAG	8 cut(s)	1036, 1647, 1717, 1924, 2100, 2326, 2482, 2659
BspHI	TCATGA	3 cut(s)	391, 736, 2800
BspLI	GGNNCC	8 cut(s)	220, 745, 746, 972, 1393, 1650, 1717, 2730
BspMAI	CTGCAG	1 cut(s)	1372
BspMI	ACCTGC	1 cut(s)	1569
BspPI	GGATC	7 cut(s)	213, 226, 1209, 1384, 1386, 1399, 2771
BspT107I	GGYRCC	1 cut(s)	1648
BsrDI	GCAATG	2 cut(s)	1429, 1795
BsrGI	TGTACA	2 cut(s)	2698, 2793
BsrI	ACTGG	8 cut(s)	362, 1062, 1409, 1444, 1717, 1840, 2057, 2775
BssECI	CCNNGG	8 cut(s)	228, 381, 609, 1242, 2066, 2351, 2375, 2424
BssMI	GATC	8 cut(s)	218, 698, 1026, 1201, 1376, 1391, 2308, 2776
BssT1I	CCWWGG	6 cut(s)	228, 381, 609, 2066, 2375, 2424
Bst2UI	CCWGG	3 cut(s)	1785, 1888, 2352
Bst4CI	ACNGT	8 cut(s)	214, 893, 1327, 1586, 2359, 2586, 2636, 2901
Bst6I	CTCTTC	5 cut(s)	747, 785, 1189, 1944, 2533
BstAUI	TGTACA	2 cut(s)	2698, 2793
BstC8I	GCNNGC	6 cut(s)	23, 910, 1162, 1516, 2574, 2744
BstDEI	CTNAG	9 cut(s)	1023, 1170, 1655, 1725, 1932, 2108, 2334, 2490, 2667
BstDSI	CCRYGG	3 cut(s)	381, 1242, 2424
BstEII	GGTNACC	1 cut(s)	2370
BstF5I	GGATG	8 cut(s)	145, 211, 283, 2017, 2053, 2506, 2744, 2779
BstKTI	GATC	8 cut(s)	221, 701, 1029, 1204, 1379, 1394, 2311, 2779
BstMAI	GTCTC	7 cut(s)	183, 227, 984, 1592, 2392, 2663, 2873
BstMBI	GATC	8 cut(s)	218, 698, 1026, 1201, 1376, 1391, 2308, 2776
BstMWI	GCNNNNNNNGC	8 cut(s)	47, 306, 1238, 1244, 1469, 1508, 1821, 2002
BstNI	CCWGG	3 cut(s)	1785, 1888, 2352
BstNSI	RCATGY	5 cut(s)	1134, 1164, 1518, 2076, 2810
BstPI	GGTNACC	1 cut(s)	2370
BstSCI	CCNGG	4 cut(s)	176, 1783, 1886, 2350
BstSFI	CTRYAG	5 cut(s)	1368, 1503, 2547, 2655, 2900
BstV1I	GCAGC	5 cut(s)	585, 748, 1495, 1498, 2106
BstV2I	GAAGAC	4 cut(s)	439, 2595, 2640, 2847
BstX2I	RGATCY	3 cut(s)	218, 1201, 1391
BstXI	CCANNNNNNTGG	2 cut(s)	1404, 1632
BstYI	RGATCY	3 cut(s)	218, 1201, 1391
BsuI	GTATCC	1 cut(s)	2619
BsuRI	GGCC	7 cut(s)	9, 89, 181, 360, 1958, 2029, 2754
BtgI	CCRYGG	3 cut(s)	381, 1242, 2424
BtrI	CACGTC	1 cut(s)	104
BtsCI	GGATG	8 cut(s)	145, 211, 283, 2017, 2053, 2506, 2744, 2779
BtsI	GCAGTG	2 cut(s)	1768, 2264
BtsIMutI	CAGTG	7 cut(s)	311, 1652, 1710, 1768, 2264, 2768, 2889
BveI	ACCTGC	1 cut(s)	1569
Cac8I	GCNNGC	6 cut(s)	23, 910, 1162, 1516, 2574, 2744
CaiI	CAGNNNCTG	3 cut(s)	878, 1584, 2357
CciI	TCATGA	3 cut(s)	391, 736, 2800
Cfr13I	GGNCC	5 cut(s)	179, 359, 744, 1690, 2027
CseI	GACGC	1 cut(s)	2129
Csp6I	GTAC	7 cut(s)	1402, 1417, 1582, 2012, 2183, 2699, 2794
CviQI	GTAC	7 cut(s)	1402, 1417, 1582, 2012, 2183, 2699, 2794
DdeI	CTNAG	9 cut(s)	1023, 1170, 1655, 1725, 1932, 2108, 2334, 2490, 2667
DpnI	GATC	8 cut(s)	220, 700, 1028, 1203, 1378, 1393, 2310, 2778
DpnII	GATC	8 cut(s)	218, 698, 1026, 1201, 1376, 1391, 2308, 2776
EaeI	YGGCCR	1 cut(s)	87
Eam1104I	CTCTTC	5 cut(s)	747, 785, 1189, 1944, 2533
EarI	CTCTTC	5 cut(s)	747, 785, 1189, 1944, 2533
Eco130I	CCWWGG	6 cut(s)	228, 381, 609, 2066, 2375, 2424
Eco147I	AGGCCT	2 cut(s)	1958, 2754
Eco47I	GGWCC	2 cut(s)	744, 1690
Eco57I	CTGAAG	3 cut(s)	441, 766, 1176
Eco91I	GGTNACC	1 cut(s)	2370
EcoO109I	RGGNCCY	1 cut(s)	744
EcoO65I	GGTNACC	1 cut(s)	2370
EcoRI	GAATTC	1 cut(s)	2438
EcoRII	CCWGG	3 cut(s)	1783, 1886, 2350
EcoT14I	CCWWGG	6 cut(s)	228, 381, 609, 2066, 2375, 2424
ErhI	CCWWGG	6 cut(s)	228, 381, 609, 2066, 2375, 2424
FaqI	GGGAC	3 cut(s)	757, 2368, 2546
FauI	CCCGC	2 cut(s)	1252, 2428
FbaI	TGATCA	1 cut(s)	2308
Fnu4HI	GCNGC	6 cut(s)	599, 762, 1239, 1509, 1512, 2120
FokI	GGATG	8 cut(s)	132, 218, 290, 2004, 2040, 2513, 2751, 2786
Fsp4HI	GCNGC	6 cut(s)	599, 762, 1239, 1509, 1512, 2120
GluI	GCNGC	6 cut(s)	599, 762, 1239, 1509, 1512, 2120
GsaI	CCCAGC	1 cut(s)	1554
HaeIII	GGCC	7 cut(s)	9, 89, 181, 360, 1958, 2029, 2754
HapII	CCGG	2 cut(s)	177, 2049
HgaI	GACGC	1 cut(s)	2129
HincII	GTYRAC	1 cut(s)	1795
HindII	GTYRAC	1 cut(s)	1795
HindIII	AAGCTT	3 cut(s)	240, 298, 2171
HpaII	CCGG	2 cut(s)	177, 2049
HphI	GGTGA	6 cut(s)	77, 299, 1025, 1332, 1681, 1964
Hpy166II	GTNNAC	4 cut(s)	1417, 1795, 2062, 2690
Hpy188III	TCNNGA	9 cut(s)	54, 392, 429, 737, 1205, 1380, 2192, 2801, 2815
Hpy8I	GTNNAC	4 cut(s)	1417, 1795, 2062, 2690
Hpy99I	CGWCG	1 cut(s)	453
HpyAV	CCTTC	5 cut(s)	734, 1382, 1486, 1814, 2354
HpyCH4III	ACNGT	8 cut(s)	214, 893, 1327, 1586, 2359, 2586, 2636, 2901
HpyCH4IV	ACGT	3 cut(s)	103, 442, 2161
HpyF10VI	GCNNNNNNNGC	8 cut(s)	47, 306, 1238, 1244, 1469, 1508, 1821, 2002
HpyF3I	CTNAG	9 cut(s)	1023, 1170, 1655, 1725, 1932, 2108, 2334, 2490, 2667
HpySE526I	ACGT	3 cut(s)	103, 442, 2161
KflI	GGGWCCC	1 cut(s)	744
Ksp22I	TGATCA	1 cut(s)	2308
Kzo9I	GATC	8 cut(s)	218, 698, 1026, 1201, 1376, 1391, 2308, 2776
LmnI	GCTCC	1 cut(s)	2422
Lsp1109I	GCAGC	5 cut(s)	585, 748, 1495, 1498, 2106
MaeII	ACGT	3 cut(s)	103, 442, 2161
MaeIII	GTNAC	8 cut(s)	65, 397, 806, 1570, 2315, 2370, 2611, 2854
MalI	GATC	8 cut(s)	220, 700, 1028, 1203, 1378, 1393, 2310, 2778
MboI	GATC	8 cut(s)	218, 698, 1026, 1201, 1376, 1391, 2308, 2776
MfeI	CAATTG	1 cut(s)	2223
MflI	RGATCY	3 cut(s)	218, 1201, 1391
MhlI	GDGCHC	1 cut(s)	311
MlsI	TGGCCA	1 cut(s)	89
MluNI	TGGCCA	1 cut(s)	89
MlyI	GAGTC	5 cut(s)	182, 229, 1283, 1567, 2654
MmeI	TCCRAC	5 cut(s)	196, 1277, 1354, 1893, 1967
Mox20I	TGGCCA	1 cut(s)	89
MroXI	GAANNNNTTC	2 cut(s)	300, 486
MscI	TGGCCA	1 cut(s)	89
MseI	TTAA	5 cut(s)	512, 866, 1928, 2127, 2286
MslI	CAYNNNNRTG	4 cut(s)	150, 1458, 1977, 2805
Msp20I	TGGCCA	1 cut(s)	89
MspA1I	CMGCKG	1 cut(s)	2666
MspI	CCGG	2 cut(s)	177, 2049
MspR9I	CCNGG	4 cut(s)	178, 1785, 1888, 2352
MunI	CAATTG	1 cut(s)	2223
Mva1269I	GAATGC	1 cut(s)	1807
MvaI	CCWGG	3 cut(s)	1785, 1888, 2352
MwoI	GCNNNNNNNGC	8 cut(s)	47, 306, 1238, 1244, 1469, 1508, 1821, 2002
NciI	CCSGG	1 cut(s)	178
NcoI	CCATGG	2 cut(s)	381, 2424
NdeII	GATC	8 cut(s)	218, 698, 1026, 1201, 1376, 1391, 2308, 2776
NlaIV	GGNNCC	8 cut(s)	220, 745, 746, 972, 1393, 1650, 1717, 2730
NmeAIII	GCCGAG	1 cut(s)	2596
NmuCI	GTSAC	3 cut(s)	65, 397, 1570
NspI	RCATGY	5 cut(s)	1134, 1164, 1518, 2076, 2810
PaeI	GCATGC	2 cut(s)	1164, 1518
PagI	TCATGA	3 cut(s)	391, 736, 2800
PceI	AGGCCT	2 cut(s)	1958, 2754
PciI	ACATGT	1 cut(s)	2072
PcsI	WCGNNNNNNNCGW	1 cut(s)	1250
PctI	GAATGC	1 cut(s)	1807
PdmI	GAANNNNTTC	2 cut(s)	300, 486
PflFI	GACNNNGTC	1 cut(s)	1753
PkrI	GCNGC	6 cut(s)	600, 763, 1240, 1510, 1513, 2121
PleI	GAGTC	5 cut(s)	181, 229, 1283, 1567, 2653
PpsI	GAGTC	5 cut(s)	181, 229, 1283, 1567, 2653
PpuMI	RGGWCCY	1 cut(s)	744
PscI	ACATGT	1 cut(s)	2072
PshBI	ATTAAT	1 cut(s)	2286
Psp1406I	AACGTT	1 cut(s)	2161
Psp5II	RGGWCCY	1 cut(s)	744
Psp6I	CCWGG	3 cut(s)	1783, 1886, 2350
PspEI	GGTNACC	1 cut(s)	2370
PspFI	CCCAGC	1 cut(s)	1550
PspGI	CCWGG	3 cut(s)	1783, 1886, 2350
PspN4I	GGNNCC	8 cut(s)	220, 745, 746, 972, 1393, 1650, 1717, 2730
PspPI	GGNCC	5 cut(s)	179, 359, 744, 1690, 2027
PspPPI	RGGWCCY	1 cut(s)	744
PstI	CTGCAG	1 cut(s)	1372
PstNI	CAGNNNCTG	3 cut(s)	878, 1584, 2357
PsuI	RGATCY	3 cut(s)	218, 1201, 1391
PsyI	GACNNNGTC	1 cut(s)	1753
PvuII	CAGCTG	1 cut(s)	2666
RsaI	GTAC	7 cut(s)	1403, 1418, 1583, 2013, 2184, 2700, 2795
RsaNI	GTAC	7 cut(s)	1402, 1417, 1582, 2012, 2183, 2699, 2794
RseI	CAYNNNNRTG	4 cut(s)	150, 1458, 1977, 2805
SaqAI	TTAA	5 cut(s)	512, 866, 1928, 2127, 2286
SatI	GCNGC	6 cut(s)	599, 762, 1239, 1509, 1512, 2120
Sau3AI	GATC	8 cut(s)	218, 698, 1026, 1201, 1376, 1391, 2308, 2776
Sau96I	GGNCC	5 cut(s)	179, 359, 744, 1690, 2027
SchI	GAGTC	5 cut(s)	182, 229, 1283, 1567, 2654
ScrFI	CCNGG	4 cut(s)	178, 1785, 1888, 2352
SduI	GDGCHC	1 cut(s)	311
SfcI	CTRYAG	5 cut(s)	1368, 1503, 2547, 2655, 2900
SinI	GGWCC	2 cut(s)	744, 1690
SmiMI	CAYNNNNRTG	4 cut(s)	150, 1458, 1977, 2805
SmlI	CTYRAG	4 cut(s)	237, 1205, 1589, 1898
SmoI	CTYRAG	4 cut(s)	237, 1205, 1589, 1898
SphI	GCATGC	2 cut(s)	1164, 1518
SseBI	AGGCCT	2 cut(s)	1958, 2754
SsiI	CCGC	4 cut(s)	1239, 1259, 1766, 2421
SspI	AATATT	2 cut(s)	930, 2508
StuI	AGGCCT	2 cut(s)	1958, 2754
StyD4I	CCNGG	4 cut(s)	176, 1783, 1886, 2350
StyI	CCWWGG	6 cut(s)	228, 381, 609, 2066, 2375, 2424
TaaI	ACNGT	8 cut(s)	214, 893, 1327, 1586, 2359, 2586, 2636, 2901
TaiI	ACGT	3 cut(s)	106, 445, 2164
TaqI	TCGA	5 cut(s)	1379, 1497, 1865, 2235, 2787
TatI	WGTACW	4 cut(s)	1401, 1416, 2698, 2793
TauI	GCSGC	1 cut(s)	1241
Tru1I	TTAA	5 cut(s)	512, 866, 1928, 2127, 2286
Tru9I	TTAA	5 cut(s)	512, 866, 1928, 2127, 2286
TscAI	CASTG	7 cut(s)	311, 1659, 1717, 1768, 2271, 2775, 2889
TseFI	GTSAC	3 cut(s)	65, 397, 1570
TseI	GCWGC	5 cut(s)	598, 761, 1508, 1511, 2119
Tsp45I	GTSAC	3 cut(s)	65, 397, 1570
TspGWI	ACGGA	1 cut(s)	451
TspRI	CASTG	7 cut(s)	311, 1659, 1717, 1768, 2271, 2775, 2889
Tth111I	GACNNNGTC	1 cut(s)	1753
VpaK11BI	GGWCC	2 cut(s)	744, 1690
VspI	ATTAAT	1 cut(s)	2286
XapI	RAATTY	3 cut(s)	376, 1741, 2438
XceI	RCATGY	5 cut(s)	1134, 1164, 1518, 2076, 2810
XmaJI	CCTAGG	1 cut(s)	2066
XmnI	GAANNNNTTC	2 cut(s)	300, 486

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.