Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0005431

Physical Location & Seq

Genomic Coordinates Forward (+)

10608130 .. 10616863

Gene Structure 8734 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000022723

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2862 bp

ATGCTAAGCAAGAAGGAGAGCAAGCGTTCCAAAGAATCAGCAGCATCGGCATCAGGAACTCTGGGTGACATATCAGAGGAGGCTGTGGCCAAACTTGTCAGACGTGCAAATAGAGGTGGGAAAAAGAAATGTGAGAATCAACTTCATCCACGTGATTTGATTGGAAAGCAAGAGTCCGGGCCACAAAGACACAAAAAAGATGTGGATGCAACAGTCGTATCCAATGCCTTGGAGACTGAAGCTTGTAGCAGAGATGCTTTGCAAAAGGTATCTAGGGATGTGAAAGGTGATGGGGAAAGCTTTCAGTGCTCTTTTACGAATAGCAGGGAAGAACTGAATGACTCAGATTGGGAAGATGGCCCAGTTCCAATTTCAAATTCCGTGGGTGGTCATGAAGTGACTATTGAAATAAATGAAACACCTGATTCCAGAAGACGGAAACGTAGTCGTCGGGCTTCGGTTGAAGATAAGGAATTGGCTGAACTTGTTCATAAAGCTCATTTACTCTGTTTAATTGCACGGGGAAGATTGATTGACAGAGCTTGTGATGATGATCTTATTCAGGCTTCCTTACTTTCTCTTCTACCAGAACACTTGCTGCATATATCCAAGGTTGCAAAACTTACTGCGAAGCATTTGCTTCCCCTAGTCTGTTGGTTTCAAAATAATTTCCATGTTAGAACTACAAGCGTGAGGACATCATTTCATTCGGCTCTGAACTTTGCCCTTGAAACTCGTGAAGGGACCCAAGAAGAGATTGCTGCATTGTCTGTGGCACTCTTCAGAGCTTTGAATCTGACAACCCGGTTGGTGTCCATTTTGAATGTTGCCTCCTTAAAACCAGAGGCTGACAAGACTGACTGTTCTAGTGAAGATGCAAGCAGATTGAGGAAGGGAATATTCAGCACTTCAACCCCAATGGTGGCTAGAACAAATGAGGTTCCTGTGTCTCCTGCTACAAGTAGTGAGAGAAACTCTGTGGGTGAAACTCCTCAGATCGGTTCATACAAAAGTAAGGACTGCCACCTTACCAGTAGCAAAACTCAGTGCAAAGGTTCTTATGTTTATGAGTTGAATGATAAAATGTCAGATACTTTAGCTTGTGAGGAATGGAATGATATGTCTGAAGCATGCCATACTAGGAAATCACAGGAATCAAAGAGAAGAGGGGATCTTGAGTTTGAAATGCAGTTGCAAATGGCTCTTTCCGCCACTGCAGTTCCGACTGCGGGTATAAAAATAGGTTCAGATAATAAAGACTCAAATGGTAATGTTGCAAAAAGATTGAAAAGAACTGTTTGTGAAGAATCACCAATTTCCTCCCAGAGTATCTCTACTGCAGTTGGATCGAGAAAAGAAGGATCCCCATTGTACTGGGCAGAAGTGTACTGCAATGGGGAAAACTTGACTGGAAAGTGGCTGCATATTGATGCTATCAATGCAATTATTGATGGAGAACAGAAGGTCGAAGCTGTAGCTGCTGCATGCAAAACGCCTTTGAGATATGTAGTTGCTTTCGCTGGGAATGGGGCTAAAGATGTGACTCGCAGGTACTGTCTCAAGTGGTATCAGATAGCATCTCAACGAGTGAATCCAATTTGGTGGGATAAGGTGTTGGCACCACTGAGAGATTTAGAGGTGAGAGCAACAGGAGGCATGGTCTATTTGGAGAAAGAACACACTGGTTCCTCATCTGAACATAATAAGGAAAATTTTTTGAACAACTCTGGCAGTGCGGAAATGAGTTATCTTCCGAGCAATGTCAACTTGAATGCGAAGTCTAGCCTAGAAGGCTCAAAAGAAACTGGAAAGGGGCTTGATGTAGAATCTTCTTCAAGGAGTTTTGAGATTGCTACCAGGAACTCCCTTGAGGATATGGAGTTGGAAACTAGGGCATTAACTGAGCCTCTTCCAACAAATCAACAGGCCTATAAAAACCATCACCTTTATGCTATTGAAAAATGGCTAACCAAGCATCAGGTACTTCATCCGAAGGGGCCAATTCTGGGGTTTTGTTCCGGTCATCCAGTTTACCCTAGGACATGTGTGCAAACTCTAATGACAAAACACAAATGGCTGAGGGAGGGGCTGCAAATTAAACCGAATGAGCGTCCTGTCAAGGAGTTGAAACGTTCTATCAAGCTTCAAAAAGTACAGGTTCTTGAAGATGATGATTATGTCGGGGGTAATTCAATTGGAACTATCGAACTTTATGGGAAGTGGCAACTTGAACCACTGCATCTGCCTCATGCTATTAATGGAAAAGTGCCAAAGAATGATCATGGTAACGTGGAGGTATGGTCTGAGAAGTGCCTTCCACCAGGGACTGTGCATTTGAGGTTACCAAGGGTGTTTTCGGTTGCCAGGAGACTAGAAATTGATTACGCTCCTGCCATGGTTGATTTTGAATTCAAAAATGGTCAATCATATCCTGTCTTTGATGGTATAGTAGTGTGTGCTGAGTTCAAGGATGCAATATTGGAGGCTTATGCAGAGGAACGGGACAGAAGAGAGGCTGTAGAAAAGAAAAGATATGAAATGCAAGCTATTTCACGGTGGTATCAGCTTCTGTCTTCCATTGTAACTCGGCAGAGGATACAAAACCGTTATGGGGAGTCTTCATCTACAGCATCAGCTGAGACTGAAAATGTGAATAGTAAACTAGATGTACAGCTTGGTGGTGGTCAGAATGATGAGGAACCTCTTGGATGCCAGCAAGAAGGCCTACATGAAAATACACCGGATGATCCTACCATCGAGCTGGACAATCATGAGCATGTATTCTTGACAGAAAATGAAGACAACTTGGTAGTTACAAAGAGGTGTCTCTGTGGATTTTCAGTGCAAGTGGAAGAACTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000109 GO:0000111 GO:0000217 GO:0000404 GO:0000405 GO:0000715 GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0003690 GO:0003697 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005739 GO:0005886 GO:0006139 GO:0006259 GO:0006281 GO:0006289 GO:0006294 GO:0006298 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007346 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009987 GO:0010564 GO:0016020 GO:0016043 GO:0022607 GO:0030983 GO:0031974 GO:0031981 GO:0032135 GO:0032991 GO:0033554 GO:0034622 GO:0034641 GO:0034644 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051276 GO:0051716 GO:0051726 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070911 GO:0070914 GO:0071214 GO:0071478 GO:0071482 GO:0071704 GO:0071824 GO:0071840 GO:0071942 GO:0071944 GO:0090304 GO:0097159 GO:0104004 GO:1901360 GO:1901363 GO:1901987 GO:1901990 GO:1990391

KEGG Pathways

Metabolic & Signaling

ko03420 map03420

Pfam Domains

Protein Families

BHD_1 BHD_2 BHD_3 Rad4 Transglut_core

Protein Analysis

954

Amino Acids

106.39

Weight (kDa)

6.32

Isoelectric Point (pI)

47.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rad4	PF03835	442 - 544	2.9e-19	Rad4 transglutaminase-like domain
BHD_1	PF10403	634 - 683	4.8e-17	Rad4 beta-hairpin domain 1
BHD_2	PF10404	688 - 749	6.9e-12	Rad4 beta-hairpin domain 2
BHD_3	PF10405	756 - 829	2.9e-26	Rad4 beta-hairpin domain 3

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005431)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16630 AT5G16630 AT5G16630
fragaria_vesca	FvH4_1g28770 FvH4_1g28770 FvH4_1g28770 FvH4_1g28770
malus_domestica	MD17G1258300.v1.1
prunus_persica	Prupe.3G161000_v2.0.a1 Prupe.3G161000_v2.0.a1 Prupe.3G161000_v2.0.a1 Prupe.3G161000_v2.0.a1
pyrus_communis	pycom17g26020
rosa_chinensis	RchiOBHm_Chr3g0493161
rosa_laevigata	RLG00000022722 RLG00000022723 RLG00000022724
rosa_multiflora	Rmu_co8401441.1_g000001 Rmu_sc0003290.1_g000009 Rmu_sc0009488.1_g000001
rosa_roxburghii	Rroxscaffold_6G00391760
rosa_rugosa	Rorug03G0259500 Rorug03G0259600 Rorug03G0259700
rosa_samantha	Rh3AG308600 Rh3BG344700 Rh3CG341900 Rh3DG344000
rosa_wichuraiana	Rw3G027140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1539
AccB1I	GGYRCC	1 cut(s)	1618
AciI	CCGC	3 cut(s)	1209, 1229, 1736
AclI	AACGTT	1 cut(s)	2131
AclWI	GGATC	5 cut(s)	1179, 1354, 1356, 1369, 2741
AcoI	YGGCCR	1 cut(s)	87
AcsI	RAATTY	3 cut(s)	376, 1711, 2408
AcuI	CTGAAG	3 cut(s)	258, 766, 1146
AcvI	CACGTG	1 cut(s)	152
AfaI	GTAC	6 cut(s)	1373, 1388, 1553, 1983, 2154, 2670
AfiI	CCNNNNNNNGG	6 cut(s)	435, 922, 998, 1229, 2006, 2611
AflIII	ACRYGT	1 cut(s)	2042
AjiI	CACGTC	1 cut(s)	104
AjnI	CCWGG	3 cut(s)	1856, 2320, 2363
AjuI	GAANNNNNNNTTGG	4 cut(s)	361, 393, 2338, 2370
Alw21I	GWGCWC	1 cut(s)	311
Alw26I	GTCTC	6 cut(s)	227, 954, 1562, 2362, 2633, 2831
AlwI	GGATC	5 cut(s)	1179, 1354, 1356, 1369, 2741
AlwNI	CAGNNNCTG	4 cut(s)	848, 1554, 2327, 2569
AoxI	GGCC	6 cut(s)	87, 179, 358, 1926, 1997, 2722
ApeKI	GCWGC	7 cut(s)	41, 598, 761, 1420, 1478, 1481, 2089
ApoI	RAATTY	3 cut(s)	376, 1711, 2408
ArsI	GACNNNNNNTTYG	2 cut(s)	2317, 2349
AseI	ATTAAT	1 cut(s)	2256
Asp700I	GAANNNNTTC	2 cut(s)	300, 486
AspA2I	CCTAGG	1 cut(s)	2036
AspS9I	GGNCC	4 cut(s)	179, 359, 744, 1997
AsuC2I	CCSGG	2 cut(s)	178, 805
AsuHPI	GGTGA	6 cut(s)	77, 299, 995, 1302, 1651, 1934
AvaII	GGWCC	1 cut(s)	744
AvrII	CCTAGG	1 cut(s)	2036
BaeI	ACNNNNGTAYC	4 cut(s)	201, 234, 2543, 2576
BalI	TGGCCA	1 cut(s)	89
BamHI	GGATCC	1 cut(s)	1361
BanI	GGYRCC	1 cut(s)	1618
BauI	CACGAG	1 cut(s)	735
BbrPI	CACGTG	1 cut(s)	152
BbsI	GAAGAC	4 cut(s)	439, 2565, 2610, 2805
Bbv12I	GWGCWC	1 cut(s)	311
BbvCI	CCTCAGC	1 cut(s)	2078
BbvI	GCAGC	7 cut(s)	53, 585, 748, 1407, 1465, 1468, 2076
BccI	CCATC	6 cut(s)	284, 350, 1445, 1947, 2435, 2762
BcgI	CGANNNNNNTGC	4 cut(s)	619, 653, 1329, 1363
BciT130I	CCWGG	3 cut(s)	1858, 2322, 2365
BciVI	GTATCC	2 cut(s)	229, 2589
BclI	TGATCA	1 cut(s)	2278
BcnI	CCSGG	2 cut(s)	178, 805
BcoDI	GTCTC	6 cut(s)	227, 954, 1562, 2362, 2633, 2831
BfmI	CTRYAG	6 cut(s)	1215, 1338, 1473, 2517, 2625, 2858
BfuAI	ACCTGC	1 cut(s)	1539
BfuI	GTATCC	2 cut(s)	229, 2589
BglI	GCCNNNNNGGC	1 cut(s)	1791
BisI	GCNGC	7 cut(s)	42, 599, 762, 1421, 1479, 1482, 2090
BlnI	CCTAGG	1 cut(s)	2036
BlpI	GCTNAGC	1 cut(s)	5
BlsI	GCNGC	7 cut(s)	43, 600, 763, 1422, 1480, 1483, 2091
Bme1390I	CCNGG	5 cut(s)	178, 805, 1858, 2322, 2365
Bme18I	GGWCC	1 cut(s)	744
BmgBI	CACGTC	1 cut(s)	104
BmgT120I	GGNCC	4 cut(s)	179, 359, 744, 1997
BmiI	GGNNCC	8 cut(s)	745, 746, 942, 1363, 1620, 1687, 1998, 2700
BmrFI	CCNGG	5 cut(s)	178, 805, 1858, 2322, 2365
BmrI	ACTGGG	2 cut(s)	356, 1384
BmuI	ACTGGG	2 cut(s)	356, 1384
BpiI	GAAGAC	4 cut(s)	439, 2565, 2610, 2805
BplI	GAGNNNNNCTC	2 cut(s)	959, 991
Bpu10I	CCTNAGC	1 cut(s)	2078
Bpu1102I	GCTNAGC	1 cut(s)	5
BpuEI	CTTGAG	3 cut(s)	1196, 1544, 1889
BpuMI	CCSGG	2 cut(s)	178, 805
BsaAI	YACGTR	1 cut(s)	152
BsaBI	GATNNNNATC	1 cut(s)	552
BsaJI	CCNNGG	7 cut(s)	228, 381, 609, 2036, 2321, 2345, 2394
BsaWI	WCCGGW	2 cut(s)	2018, 2740
BsaXI	ACNNNNNCTCC	4 cut(s)	1644, 1674, 2285, 2315
Bsc4I	CCNNNNNNNGG	6 cut(s)	435, 922, 998, 1229, 2006, 2611
Bse1I	ACTGG	7 cut(s)	362, 1032, 1379, 1414, 1687, 1810, 2027
Bse3DI	GCAATG	2 cut(s)	1399, 1765
Bse8I	GATNNNNATC	1 cut(s)	552
BseBI	CCWGG	3 cut(s)	1858, 2322, 2365
BseDI	CCNNGG	7 cut(s)	228, 381, 609, 2036, 2321, 2345, 2394
BseGI	GGATG	8 cut(s)	145, 211, 283, 1987, 2023, 2476, 2714, 2749
BseJI	GATNNNNATC	1 cut(s)	552
BseLI	CCNNNNNNNGG	6 cut(s)	435, 922, 998, 1229, 2006, 2611
BseMI	GCAATG	2 cut(s)	1399, 1765
BseMII	CTCAG	9 cut(s)	357, 1007, 1058, 1616, 1893, 2069, 2295, 2451, 2628
BseNI	ACTGG	7 cut(s)	362, 1032, 1379, 1414, 1687, 1810, 2027
BseRI	GAGGAG	2 cut(s)	92, 981
BseXI	GCAGC	7 cut(s)	53, 585, 748, 1407, 1465, 1468, 2076
BseYI	CCCAGC	1 cut(s)	1520
BshFI	GGCC	6 cut(s)	89, 181, 360, 1928, 1999, 2724
BshNI	GGYRCC	1 cut(s)	1618
BsiHKAI	GWGCWC	1 cut(s)	311
BsiSI	CCGG	4 cut(s)	177, 805, 2019, 2741
BslFI	GGGAC	3 cut(s)	757, 2338, 2516
BslI	CCNNNNNNNGG	6 cut(s)	435, 922, 998, 1229, 2006, 2611
BsmAI	GTCTC	6 cut(s)	227, 954, 1562, 2362, 2633, 2831
BsmFI	GGGAC	3 cut(s)	757, 2338, 2516
BsmI	GAATGC	1 cut(s)	1777
BsnI	GGCC	6 cut(s)	89, 181, 360, 1928, 1999, 2724
Bsp1286I	GDGCHC	1 cut(s)	311
Bsp1407I	TGTACA	1 cut(s)	2668
Bsp143I	GATC	7 cut(s)	553, 996, 1171, 1346, 1361, 2278, 2746
Bsp1720I	GCTNAGC	1 cut(s)	5
Bsp19I	CCATGG	1 cut(s)	2394
BspACI	CCGC	3 cut(s)	1209, 1229, 1736
BspANI	GGCC	6 cut(s)	89, 181, 360, 1928, 1999, 2724
BspCNI	CTCAG	9 cut(s)	356, 1006, 1057, 1617, 1894, 2070, 2296, 2452, 2629
BspHI	TCATGA	2 cut(s)	391, 2770
BspLI	GGNNCC	8 cut(s)	745, 746, 942, 1363, 1620, 1687, 1998, 2700
BspMAI	CTGCAG	2 cut(s)	1219, 1342
BspMI	ACCTGC	1 cut(s)	1539
BspPI	GGATC	5 cut(s)	1179, 1354, 1356, 1369, 2741
BspT107I	GGYRCC	1 cut(s)	1618
BsrDI	GCAATG	2 cut(s)	1399, 1765
BsrGI	TGTACA	1 cut(s)	2668
BsrI	ACTGG	7 cut(s)	362, 1032, 1379, 1414, 1687, 1810, 2027
BssECI	CCNNGG	7 cut(s)	228, 381, 609, 2036, 2321, 2345, 2394
BssMI	GATC	7 cut(s)	553, 996, 1171, 1346, 1361, 2278, 2746
BssSI	CACGAG	1 cut(s)	735
BssT1I	CCWWGG	5 cut(s)	228, 609, 2036, 2345, 2394
Bst2BI	CACGAG	1 cut(s)	735
Bst2UI	CCWGG	3 cut(s)	1858, 2322, 2365
Bst4CI	ACNGT	8 cut(s)	214, 863, 1297, 1556, 2329, 2556, 2606, 2859
Bst6I	CTCTTC	6 cut(s)	585, 747, 785, 1159, 1914, 2503
BstAUI	TGTACA	1 cut(s)	2668
BstBAI	YACGTR	1 cut(s)	152
BstC8I	GCNNGC	6 cut(s)	23, 880, 1132, 1486, 2544, 2714
BstDSI	CCRYGG	2 cut(s)	381, 2394
BstEII	GGTNACC	1 cut(s)	2340
BstF5I	GGATG	8 cut(s)	145, 211, 283, 1987, 2023, 2476, 2714, 2749
BstKTI	GATC	7 cut(s)	556, 999, 1174, 1349, 1364, 2281, 2749
BstMAI	GTCTC	6 cut(s)	227, 954, 1562, 2362, 2633, 2831
BstMBI	GATC	7 cut(s)	553, 996, 1171, 1346, 1361, 2278, 2746
BstMWI	GCNNNNNNNGC	7 cut(s)	47, 306, 1208, 1439, 1478, 1791, 1972
BstNI	CCWGG	3 cut(s)	1858, 2322, 2365
BstNSI	RCATGY	4 cut(s)	1134, 1488, 2046, 2780
BstPI	GGTNACC	1 cut(s)	2340
BstSCI	CCNGG	5 cut(s)	176, 803, 1856, 2320, 2363
BstSFI	CTRYAG	6 cut(s)	1215, 1338, 1473, 2517, 2625, 2858
BstV1I	GCAGC	7 cut(s)	53, 585, 748, 1407, 1465, 1468, 2076
BstV2I	GAAGAC	4 cut(s)	439, 2565, 2610, 2805
BstX2I	RGATCY	2 cut(s)	1171, 1361
BstXI	CCANNNNNNTGG	4 cut(s)	229, 1374, 1602, 2761
BstYI	RGATCY	2 cut(s)	1171, 1361
BsuI	GTATCC	2 cut(s)	229, 2589
BsuRI	GGCC	6 cut(s)	89, 181, 360, 1928, 1999, 2724
BtgI	CCRYGG	2 cut(s)	381, 2394
BtrI	CACGTC	1 cut(s)	104
BtsCI	GGATG	8 cut(s)	145, 211, 283, 1987, 2023, 2476, 2714, 2749
BtsI	GCAGTG	3 cut(s)	1212, 1738, 2234
BtsIMutI	CAGTG	8 cut(s)	311, 1052, 1212, 1622, 1680, 1738, 2234, 2847
BveI	ACCTGC	1 cut(s)	1539
Cac8I	GCNNGC	6 cut(s)	23, 880, 1132, 1486, 2544, 2714
CaiI	CAGNNNCTG	4 cut(s)	848, 1554, 2327, 2569
CciI	TCATGA	2 cut(s)	391, 2770
Cfr13I	GGNCC	4 cut(s)	179, 359, 744, 1997
CseI	GACGC	1 cut(s)	2099
Csp6I	GTAC	6 cut(s)	1372, 1387, 1552, 1982, 2153, 2669
CspCI	CAANNNNNGTGG	4 cut(s)	138, 173, 363, 398
CviQI	GTAC	6 cut(s)	1372, 1387, 1552, 1982, 2153, 2669
DpnI	GATC	7 cut(s)	555, 998, 1173, 1348, 1363, 2280, 2748
DpnII	GATC	7 cut(s)	553, 996, 1171, 1346, 1361, 2278, 2746
EaeI	YGGCCR	1 cut(s)	87
Eam1104I	CTCTTC	6 cut(s)	585, 747, 785, 1159, 1914, 2503
EarI	CTCTTC	6 cut(s)	585, 747, 785, 1159, 1914, 2503
EciI	GGCGGA	1 cut(s)	1198
Eco130I	CCWWGG	5 cut(s)	228, 609, 2036, 2345, 2394
Eco147I	AGGCCT	2 cut(s)	1928, 2724
Eco47I	GGWCC	1 cut(s)	744
Eco57I	CTGAAG	3 cut(s)	258, 766, 1146
Eco72I	CACGTG	1 cut(s)	152
Eco91I	GGTNACC	1 cut(s)	2340
EcoO109I	RGGNCCY	1 cut(s)	744
EcoO65I	GGTNACC	1 cut(s)	2340
EcoRI	GAATTC	1 cut(s)	2408
EcoRII	CCWGG	3 cut(s)	1856, 2320, 2363
EcoT14I	CCWWGG	5 cut(s)	228, 609, 2036, 2345, 2394
ErhI	CCWWGG	5 cut(s)	228, 609, 2036, 2345, 2394
FaqI	GGGAC	3 cut(s)	757, 2338, 2516
FauI	CCCGC	1 cut(s)	1222
FbaI	TGATCA	1 cut(s)	2278
Fnu4HI	GCNGC	7 cut(s)	42, 599, 762, 1421, 1479, 1482, 2090
FokI	GGATG	8 cut(s)	132, 218, 290, 1974, 2010, 2483, 2721, 2756
Fsp4HI	GCNGC	7 cut(s)	42, 599, 762, 1421, 1479, 1482, 2090
GluI	GCNGC	7 cut(s)	42, 599, 762, 1421, 1479, 1482, 2090
GsaI	CCCAGC	1 cut(s)	1524
HaeIII	GGCC	6 cut(s)	89, 181, 360, 1928, 1999, 2724
HapII	CCGG	4 cut(s)	177, 805, 2019, 2741
HgaI	GACGC	1 cut(s)	2099
HincII	GTYRAC	1 cut(s)	1765
HindII	GTYRAC	1 cut(s)	1765
HindIII	AAGCTT	3 cut(s)	240, 298, 2141
HpaII	CCGG	4 cut(s)	177, 805, 2019, 2741
HphI	GGTGA	6 cut(s)	77, 299, 995, 1302, 1651, 1934
Hpy166II	GTNNAC	4 cut(s)	1387, 1765, 2032, 2660
Hpy188III	TCNNGA	9 cut(s)	54, 392, 429, 737, 1175, 1350, 2162, 2771, 2785
Hpy8I	GTNNAC	4 cut(s)	1387, 1765, 2032, 2660
Hpy99I	CGWCG	1 cut(s)	453
HpyAV	CCTTC	9 cut(s)	7, 734, 886, 1352, 1456, 1784, 1987, 2324, 2714
HpyCH4III	ACNGT	8 cut(s)	214, 863, 1297, 1556, 2329, 2556, 2606, 2859
HpyCH4IV	ACGT	5 cut(s)	103, 151, 442, 2131, 2289
HpyF10VI	GCNNNNNNNGC	7 cut(s)	47, 306, 1208, 1439, 1478, 1791, 1972
HpySE526I	ACGT	5 cut(s)	103, 151, 442, 2131, 2289
KflI	GGGWCCC	1 cut(s)	744
Ksp22I	TGATCA	1 cut(s)	2278
Kzo9I	GATC	7 cut(s)	553, 996, 1171, 1346, 1361, 2278, 2746
LmnI	GCTCC	1 cut(s)	2392
Lsp1109I	GCAGC	7 cut(s)	53, 585, 748, 1407, 1465, 1468, 2076
MaeII	ACGT	5 cut(s)	103, 151, 442, 2131, 2289
MaeIII	GTNAC	7 cut(s)	65, 397, 1540, 2285, 2340, 2581, 2812
MalI	GATC	7 cut(s)	555, 998, 1173, 1348, 1363, 2280, 2748
MboI	GATC	7 cut(s)	553, 996, 1171, 1346, 1361, 2278, 2746
MfeI	CAATTG	1 cut(s)	2193
MflI	RGATCY	2 cut(s)	1171, 1361
MhlI	GDGCHC	1 cut(s)	311
MlsI	TGGCCA	1 cut(s)	89
MluNI	TGGCCA	1 cut(s)	89
MlyI	GAGTC	5 cut(s)	182, 335, 1253, 1537, 2624
MmeI	TCCRAC	4 cut(s)	1247, 1324, 1863, 1937
Mox20I	TGGCCA	1 cut(s)	89
MroXI	GAANNNNTTC	2 cut(s)	300, 486
MscI	TGGCCA	1 cut(s)	89
MseI	TTAA	5 cut(s)	512, 836, 1898, 2097, 2256
MslI	CAYNNNNRTG	4 cut(s)	150, 1428, 1947, 2775
Msp20I	TGGCCA	1 cut(s)	89
MspA1I	CMGCKG	1 cut(s)	2636
MspI	CCGG	4 cut(s)	177, 805, 2019, 2741
MspR9I	CCNGG	5 cut(s)	178, 805, 1858, 2322, 2365
MunI	CAATTG	1 cut(s)	2193
Mva1269I	GAATGC	1 cut(s)	1777
MvaI	CCWGG	3 cut(s)	1858, 2322, 2365
MwoI	GCNNNNNNNGC	7 cut(s)	47, 306, 1208, 1439, 1478, 1791, 1972
NciI	CCSGG	2 cut(s)	178, 805
NcoI	CCATGG	1 cut(s)	2394
NdeII	GATC	7 cut(s)	553, 996, 1171, 1346, 1361, 2278, 2746
NlaIV	GGNNCC	8 cut(s)	745, 746, 942, 1363, 1620, 1687, 1998, 2700
NmeAIII	GCCGAG	1 cut(s)	2566
NmuCI	GTSAC	3 cut(s)	65, 397, 1540
NspI	RCATGY	4 cut(s)	1134, 1488, 2046, 2780
PaeI	GCATGC	2 cut(s)	1134, 1488
PagI	TCATGA	2 cut(s)	391, 2770
PceI	AGGCCT	2 cut(s)	1928, 2724
PciI	ACATGT	1 cut(s)	2042
PctI	GAATGC	1 cut(s)	1777
PdmI	GAANNNNTTC	2 cut(s)	300, 486
PfeI	GAWTC	8 cut(s)	35, 136, 425, 793, 1154, 1307, 1591, 1826
PkrI	GCNGC	7 cut(s)	43, 600, 763, 1422, 1480, 1483, 2091
PleI	GAGTC	5 cut(s)	181, 335, 1253, 1537, 2623
PmaCI	CACGTG	1 cut(s)	152
PmlI	CACGTG	1 cut(s)	152
PpsI	GAGTC	5 cut(s)	181, 335, 1253, 1537, 2623
Ppu21I	YACGTR	1 cut(s)	152
PpuMI	RGGWCCY	1 cut(s)	744
PscI	ACATGT	1 cut(s)	2042
PshBI	ATTAAT	1 cut(s)	2256
Psp1406I	AACGTT	1 cut(s)	2131
Psp5II	RGGWCCY	1 cut(s)	744
Psp6I	CCWGG	3 cut(s)	1856, 2320, 2363
PspCI	CACGTG	1 cut(s)	152
PspEI	GGTNACC	1 cut(s)	2340
PspFI	CCCAGC	1 cut(s)	1520
PspGI	CCWGG	3 cut(s)	1856, 2320, 2363
PspN4I	GGNNCC	8 cut(s)	745, 746, 942, 1363, 1620, 1687, 1998, 2700
PspPI	GGNCC	4 cut(s)	179, 359, 744, 1997
PspPPI	RGGWCCY	1 cut(s)	744
PstI	CTGCAG	2 cut(s)	1219, 1342
PstNI	CAGNNNCTG	4 cut(s)	848, 1554, 2327, 2569
PsuI	RGATCY	2 cut(s)	1171, 1361
PvuII	CAGCTG	1 cut(s)	2636
RsaI	GTAC	6 cut(s)	1373, 1388, 1553, 1983, 2154, 2670
RsaNI	GTAC	6 cut(s)	1372, 1387, 1552, 1982, 2153, 2669
RseI	CAYNNNNRTG	4 cut(s)	150, 1428, 1947, 2775
SaqAI	TTAA	5 cut(s)	512, 836, 1898, 2097, 2256
SatI	GCNGC	7 cut(s)	42, 599, 762, 1421, 1479, 1482, 2090
Sau3AI	GATC	7 cut(s)	553, 996, 1171, 1346, 1361, 2278, 2746
Sau96I	GGNCC	4 cut(s)	179, 359, 744, 1997
SchI	GAGTC	5 cut(s)	182, 335, 1253, 1537, 2624
ScrFI	CCNGG	5 cut(s)	178, 805, 1858, 2322, 2365
SduI	GDGCHC	1 cut(s)	311
SfcI	CTRYAG	6 cut(s)	1215, 1338, 1473, 2517, 2625, 2858
SinI	GGWCC	1 cut(s)	744
SmiMI	CAYNNNNRTG	4 cut(s)	150, 1428, 1947, 2775
SmlI	CTYRAG	3 cut(s)	1175, 1559, 1868
SmoI	CTYRAG	3 cut(s)	1175, 1559, 1868
SphI	GCATGC	2 cut(s)	1134, 1488
SseBI	AGGCCT	2 cut(s)	1928, 2724
SsiI	CCGC	3 cut(s)	1209, 1229, 1736
SspI	AATATT	2 cut(s)	900, 2478
StuI	AGGCCT	2 cut(s)	1928, 2724
StyD4I	CCNGG	5 cut(s)	176, 803, 1856, 2320, 2363
StyI	CCWWGG	5 cut(s)	228, 609, 2036, 2345, 2394
TaaI	ACNGT	8 cut(s)	214, 863, 1297, 1556, 2329, 2556, 2606, 2859
TaiI	ACGT	5 cut(s)	106, 154, 445, 2134, 2292
TaqI	TCGA	4 cut(s)	1349, 1467, 2205, 2757
TatI	WGTACW	4 cut(s)	1371, 1386, 2152, 2668
TfiI	GAWTC	8 cut(s)	35, 136, 425, 793, 1154, 1307, 1591, 1826
Tru1I	TTAA	5 cut(s)	512, 836, 1898, 2097, 2256
Tru9I	TTAA	5 cut(s)	512, 836, 1898, 2097, 2256
TscAI	CASTG	8 cut(s)	311, 1052, 1219, 1629, 1687, 1738, 2241, 2847
TseFI	GTSAC	3 cut(s)	65, 397, 1540
TseI	GCWGC	7 cut(s)	41, 598, 761, 1420, 1478, 1481, 2089
Tsp45I	GTSAC	3 cut(s)	65, 397, 1540
TspGWI	ACGGA	2 cut(s)	370, 451
TspRI	CASTG	8 cut(s)	311, 1052, 1219, 1629, 1687, 1738, 2241, 2847
VpaK11BI	GGWCC	1 cut(s)	744
VspI	ATTAAT	1 cut(s)	2256
XapI	RAATTY	3 cut(s)	376, 1711, 2408
XceI	RCATGY	4 cut(s)	1134, 1488, 2046, 2780
XmaJI	CCTAGG	1 cut(s)	2036
XmnI	GAANNNNTTC	2 cut(s)	300, 486

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.