Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0005431

Physical Location & Seq

Genomic Coordinates Forward (+)

10574220 .. 10583871

Gene Structure 9652 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00391760.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2760 bp

ATGTGCTTGTTTATCCTTTGGGCAAGTAAAGGAACTCTGGGTGACATATCAGAGGAGGCTGTGGCCAAACTTGTCAGACGTGCAAATAGAAGTGGGAAAAAGAAATGTGAGAATCAACTTCATCCATCAAGAGTCTGGGCCACAAAGAGAAAAAAAGATGTGGATGCAACAGTCGGATCCAATGCCTTGGAGACTGAGGCTTGTAGCAGAGATGCTTTGCAAAAGGTATCTAGGGATGCGAAAGGTGATGGGGAAAGCTTTCAGTGCTCTTTTACGAATAGCAGGGAAGAACTGAATGACTCAGATTGGGAAGATGGCCCAGTTCCAATTTCAAATTCCATGGGTGGTCATGAAGTGACTATTGAAATAAATGAAACACCTGATTCCAGAAGACGGAAACGTAGTCGTCGGGCTTCGGTTGAAGATAAGGAATTGGCTGAACTTGTTCATAAAGCTCATTTACTCTGTTTAATTGCACGGGGAAGATTGATTGACAGAGCTTGTGATGATGCTCTTATTCAGTTTCAAAATAATTTCCGTGTTAGAACTACAAGTGTGAGGAGATCATTTCATTCGGCTCTGAACTTTGCCCTTGAAACTCGTGAAGGGACCCAAGAAGAGATTGCTGCATTGTCTGTGGCACTCTTCAGAGCTTTGAATCTGACAACCCGTTGTATAGGGAGGCTGGGGGTGACTTGGGAAGCACAAGTACAGATTTCTGCTCTGTTTGCTCATAACCATCCTGGTATTGGAAAAGGGAAGAATGCCCAAGTGTTGTGGAGGTTGGTGTCCATTTTGAATGTTGCCTCCTTAAAACCAGAGGCTGACAAGACTGACTGTTCTAGTGAAGATGCAAGCAGATTGAGTAAGGGAATATTCAGCACTTCAACCCCAATGGTGGCTAGAAGAAATGAGGTTCCTGTGTCTCCTGCTACAAGTAGTGAGAGAAACTCTGTCGGGGAAACTCCTCAGATCAGTTCATACAAAAGTAAGGACTGCCACCTTACCAGTAGCAAAACTCGGTGCAAAGGTTCTTATGTTTATGAGTTGAATGATAAAAGGTCAGATACTTTAGCTTGTGAGGAATGGAATGATATGTCTGAAGCATGCCATACTAAGAAATCACCGGAATCAAAGAGAAGAGGGGATCTTGAGTTTGAAATGCAGTTGCAAATGGCTCTTTCCGCCACGGCAGTTCCGACTACGGGGATAAAAAATAGTATCTCTACAGCAGTTGGATCGAGAAAAGAAGGATCCCCATTGTACTGGGCAGAAGTGTACTGCAATGGGGAAAACTTGACTGGAAAGTGGCTGCATATTGACGCTATCAATGGAATTATTGATGGAGAACAGAAGGTCGAAGCTGTAGCTGCTGCATGCAAAACGCCTTTGAGATATGTAGTTGCTTTCGCTGGGAATGGGGCTAAAGATGTGACTCGCAGGTACTGTCTCAAGTGGTATCAGATAGCATCTCAACGAGTGAATCCAATTTGGTGGGATAAGGTATTGGCACCACTGAGAGATTTAGAGGTGAGAGCAACAGGAGGCATGGTCTATTTGGAGAAAGAACACACTGGTTCCTCATCTGAACATAATAAAGAAATTTTTTTGAACAAGTCTGGCAGTGCGGAAATGAATTTTCTTCCAAGCAATGTCAACTTGAATGCGAAGTCTAGCCTAGAAGGCTCAAAAGAAACTGGAAAGGGGCTTGATGTAGAATCTTCTTCGAGGAGTTTTGAGATTGCTACCAGGAACTCCCTTGAGGATATGGAGTTGGAAACTAGGGCATTAACTGAGCCTCTTCCAACGAATCAACAGGTAGTGAATTGGCCTATAAAAAACCATCACCTTTATGCTATTGAAAAATGGCTTACCAAGCATCAGATACTTCATCCGAAAGGGCCAATTCTGGGGTTTTGTTCCGGTCATCCAGTTTACCCTAGGACATGTGTGCAAACTCTAAAGACAAAGCACAAATGCCTCACTCCTCCCATGTTGATTCTCCAGGAGTTGAAACGTTCTATCAAGCTTCAAAAAGTACAGGTTGTTGAAGATGATGATTATGTCGGGAGTAATTCAATTGGAACTATCGAACTTTATGGGAAGTGGCAACTTGAACCACTGCATCTGCCCCATGCTATTAATGGAAAAGTGCCAAAGAATGATCATGGTAACGTGGAAGTATGGTCTGAGAAGTGCCTTCCACCAGGGACTGTGCATTTGAGGTTACCAAGGGTGTTTACGGTTGCCAAGAGACTAGAAATTGATTACGCTCCCGCCATGGTTGATTTTGAATTCAAAAATGGTCAATCATATCCTGTCTTTGATGGTATAGTGGTGTGTGCTGAGTTCAAGGATGCAATATTGGAGGCTTATGCAGAGGAACGGGACAGAAGAGAGGTTGTAGAAAAGAAAAGATATGAAATGCAAGCTATTTCACGGTGGTATCAGCTTCTGTCTTCCATTGTAACTCGGCAGAGGATACAAAACCGTTATGGGGAATCTTCATCTACAGCATCAGCTGAGACTGAAAATGTGAATAGTAAACTAGATGTACAGCTTGGTGGTGGTCAGAATGATGAGGAACCTCTTGGATGCCAGCAAGGAGGCCTACATGAAAATACACCGGATGATCCTACCATCGAGCTGGACAATCATGAGCATGTATTCTTGACAGAAAACCAGAGTTTTGATGAAGACAACTTGGTAGTTACAAAGAGGTGTCTGTGTGGATTTTCAGTGCAAGTGGAAGAACTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000109 GO:0000111 GO:0000217 GO:0000404 GO:0000405 GO:0000715 GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0003690 GO:0003697 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005739 GO:0005886 GO:0006139 GO:0006259 GO:0006281 GO:0006289 GO:0006294 GO:0006298 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007346 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009987 GO:0010564 GO:0016020 GO:0016043 GO:0022607 GO:0030983 GO:0031974 GO:0031981 GO:0032135 GO:0032991 GO:0033554 GO:0034622 GO:0034641 GO:0034644 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051276 GO:0051716 GO:0051726 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070911 GO:0070914 GO:0071214 GO:0071478 GO:0071482 GO:0071704 GO:0071824 GO:0071840 GO:0071942 GO:0071944 GO:0090304 GO:0097159 GO:0104004 GO:1901360 GO:1901363 GO:1901987 GO:1901990 GO:1990391

KEGG Pathways

Metabolic & Signaling

ko03420 map03420

Pfam Domains

Protein Families

BHD_1 BHD_2 BHD_3 Rad4 Transglut_core

Protein Analysis

919

Amino Acids

102.83

Weight (kDa)

6.61

Isoelectric Point (pI)

46.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rad4	PF03835	411 - 508	3.5e-19	Rad4 transglutaminase-like domain
BHD_1	PF10403	599 - 651	6.5e-12	Rad4 beta-hairpin domain 1
BHD_3	PF10405	718 - 791	3.5e-26	Rad4 beta-hairpin domain 3

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005431)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16630 AT5G16630 AT5G16630
fragaria_vesca	FvH4_1g28770 FvH4_1g28770 FvH4_1g28770 FvH4_1g28770
malus_domestica	MD17G1258300.v1.1
prunus_persica	Prupe.3G161000_v2.0.a1 Prupe.3G161000_v2.0.a1 Prupe.3G161000_v2.0.a1 Prupe.3G161000_v2.0.a1
pyrus_communis	pycom17g26020
rosa_chinensis	RchiOBHm_Chr3g0493161
rosa_laevigata	RLG00000022722 RLG00000022723 RLG00000022724
rosa_multiflora	Rmu_co8401441.1_g000001 Rmu_sc0003290.1_g000009 Rmu_sc0009488.1_g000001
rosa_roxburghii	Rroxscaffold_6G00391760
rosa_rugosa	Rorug03G0259500 Rorug03G0259600 Rorug03G0259700
rosa_samantha	Rh3AG308600 Rh3BG344700 Rh3CG341900 Rh3DG344000
rosa_wichuraiana	Rw3G027140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1431
AccB1I	GGYRCC	1 cut(s)	1510
AciI	CCGC	3 cut(s)	1185, 1628, 2277
AclI	AACGTT	1 cut(s)	2017
AclWI	GGATC	7 cut(s)	171, 184, 1155, 1246, 1248, 1261, 2627
AcoI	YGGCCR	1 cut(s)	63
AcsI	RAATTY	4 cut(s)	334, 1602, 1636, 2294
AcuI	CTGAAG	2 cut(s)	631, 1122
AfaI	GTAC	6 cut(s)	711, 1265, 1280, 1445, 2040, 2556
AfiI	CCNNNNNNNGG	6 cut(s)	393, 749, 898, 1205, 1910, 2497
AflIII	ACRYGT	1 cut(s)	1946
AjiI	CACGTC	1 cut(s)	80
AjnI	CCWGG	4 cut(s)	742, 1748, 2004, 2206
AjuI	GAANNNNNNNTTGG	2 cut(s)	319, 351
Alw21I	GWGCWC	1 cut(s)	269
Alw26I	GTCTC	5 cut(s)	185, 930, 1454, 2248, 2519
AlwI	GGATC	7 cut(s)	171, 184, 1155, 1246, 1248, 1261, 2627
AlwNI	CAGNNNCTG	4 cut(s)	824, 1446, 2213, 2455
AoxI	GGCC	6 cut(s)	63, 138, 316, 1829, 1901, 2608
ApeKI	GCWGC	4 cut(s)	626, 1312, 1370, 1373
ApoI	RAATTY	4 cut(s)	334, 1602, 1636, 2294
ArsI	GACNNNNNNTTYG	2 cut(s)	2203, 2235
AseI	ATTAAT	1 cut(s)	2142
Asp700I	GAANNNNTTC	2 cut(s)	258, 444
AspA2I	CCTAGG	1 cut(s)	1940
AspS9I	GGNCC	4 cut(s)	138, 317, 609, 1901
AsuHPI	GGTGA	6 cut(s)	53, 257, 703, 1116, 1543, 1838
AvaII	GGWCC	1 cut(s)	609
AvrII	CCTAGG	1 cut(s)	1940
BaeI	ACNNNNGTAYC	2 cut(s)	2429, 2462
BalI	TGGCCA	1 cut(s)	65
BamHI	GGATCC	2 cut(s)	176, 1253
BanI	GGYRCC	1 cut(s)	1510
BarI	GAAGNNNNNNTAC	2 cut(s)	693, 725
BauI	CACGAG	1 cut(s)	600
BbsI	GAAGAC	3 cut(s)	397, 2451, 2703
Bbv12I	GWGCWC	1 cut(s)	269
BbvI	GCAGC	4 cut(s)	613, 1299, 1357, 1360
BccI	CCATC	8 cut(s)	133, 242, 308, 747, 1337, 1851, 2321, 2648
BceAI	ACGGC	1 cut(s)	1206
BcgI	CGANNNNNNTGC	2 cut(s)	1221, 1255
BciT130I	CCWGG	4 cut(s)	744, 1750, 2006, 2208
BciVI	GTATCC	1 cut(s)	2475
BclI	TGATCA	1 cut(s)	2164
BcoDI	GTCTC	5 cut(s)	185, 930, 1454, 2248, 2519
BfaI	CTAG	9 cut(s)	231, 843, 903, 1674, 1679, 1782, 1941, 2258, 2549
BfmI	CTRYAG	4 cut(s)	1227, 1365, 2511, 2756
BfuAI	ACCTGC	1 cut(s)	1431
BfuI	GTATCC	1 cut(s)	2475
BglI	GCCNNNNNGGC	1 cut(s)	1683
BisI	GCNGC	4 cut(s)	627, 1313, 1371, 1374
BlnI	CCTAGG	1 cut(s)	1940
BlsI	GCNGC	4 cut(s)	628, 1314, 1372, 1375
Bme1390I	CCNGG	4 cut(s)	744, 1750, 2006, 2208
Bme18I	GGWCC	1 cut(s)	609
BmgBI	CACGTC	1 cut(s)	80
BmgT120I	GGNCC	4 cut(s)	138, 317, 609, 1901
BmiI	GGNNCC	8 cut(s)	178, 610, 611, 918, 1255, 1512, 1579, 2586
BmrFI	CCNGG	4 cut(s)	744, 1750, 2006, 2208
BmrI	ACTGGG	2 cut(s)	314, 1276
BmuI	ACTGGG	2 cut(s)	314, 1276
BpiI	GAAGAC	3 cut(s)	397, 2451, 2703
BplI	GAGNNNNNCTC	2 cut(s)	935, 967
BpmI	CTGGAG	1 cut(s)	1988
BpuEI	CTTGAG	3 cut(s)	1172, 1436, 1781
BsaJI	CCNNGG	7 cut(s)	186, 339, 1188, 1940, 2207, 2231, 2280
BsaWI	WCCGGW	3 cut(s)	1126, 1922, 2626
BsaXI	ACNNNNNCTCC	2 cut(s)	1536, 1566
Bsc4I	CCNNNNNNNGG	6 cut(s)	393, 749, 898, 1205, 1910, 2497
Bse1I	ACTGG	7 cut(s)	320, 1008, 1271, 1306, 1579, 1702, 1931
Bse3DI	GCAATG	2 cut(s)	1291, 1657
BseBI	CCWGG	4 cut(s)	744, 1750, 2006, 2208
BseDI	CCNNGG	7 cut(s)	186, 339, 1188, 1940, 2207, 2231, 2280
BseGI	GGATG	9 cut(s)	121, 169, 241, 739, 1891, 1927, 2362, 2600, 2635
BseLI	CCNNNNNNNGG	6 cut(s)	393, 749, 898, 1205, 1910, 2497
BseMI	GCAATG	2 cut(s)	1291, 1657
BseMII	CTCAG	8 cut(s)	186, 315, 983, 1508, 1785, 2181, 2337, 2514
BseNI	ACTGG	7 cut(s)	320, 1008, 1271, 1306, 1579, 1702, 1931
BseRI	GAGGAG	5 cut(s)	68, 574, 957, 1744, 1977
BseXI	GCAGC	4 cut(s)	613, 1299, 1357, 1360
BseYI	CCCAGC	2 cut(s)	685, 1412
BshFI	GGCC	6 cut(s)	65, 140, 318, 1831, 1903, 2610
BshNI	GGYRCC	1 cut(s)	1510
BsiHKAI	GWGCWC	1 cut(s)	269
BsiSI	CCGG	3 cut(s)	1127, 1923, 2627
BslFI	GGGAC	3 cut(s)	622, 2224, 2402
BslI	CCNNNNNNNGG	6 cut(s)	393, 749, 898, 1205, 1910, 2497
BsmAI	GTCTC	5 cut(s)	185, 930, 1454, 2248, 2519
BsmFI	GGGAC	3 cut(s)	622, 2224, 2402
BsmI	GAATGC	2 cut(s)	769, 1669
BsnI	GGCC	6 cut(s)	65, 140, 318, 1831, 1903, 2610
Bsp1286I	GDGCHC	1 cut(s)	269
Bsp1407I	TGTACA	1 cut(s)	2554
Bsp143I	GATC	8 cut(s)	176, 563, 972, 1147, 1238, 1253, 2164, 2632
Bsp19I	CCATGG	2 cut(s)	339, 2280
BspACI	CCGC	3 cut(s)	1185, 1628, 2277
BspANI	GGCC	6 cut(s)	65, 140, 318, 1831, 1903, 2610
BspCNI	CTCAG	8 cut(s)	187, 314, 982, 1509, 1786, 2182, 2338, 2515
BspHI	TCATGA	2 cut(s)	349, 2656
BspLI	GGNNCC	8 cut(s)	178, 610, 611, 918, 1255, 1512, 1579, 2586
BspMI	ACCTGC	1 cut(s)	1431
BspPI	GGATC	7 cut(s)	171, 184, 1155, 1246, 1248, 1261, 2627
BspT107I	GGYRCC	1 cut(s)	1510
BsrDI	GCAATG	2 cut(s)	1291, 1657
BsrGI	TGTACA	1 cut(s)	2554
BsrI	ACTGG	7 cut(s)	320, 1008, 1271, 1306, 1579, 1702, 1931
BssECI	CCNNGG	7 cut(s)	186, 339, 1188, 1940, 2207, 2231, 2280
BssMI	GATC	8 cut(s)	176, 563, 972, 1147, 1238, 1253, 2164, 2632
BssSI	CACGAG	1 cut(s)	600
BssT1I	CCWWGG	5 cut(s)	186, 339, 1940, 2231, 2280
Bst2BI	CACGAG	1 cut(s)	600
Bst2UI	CCWGG	4 cut(s)	744, 1750, 2006, 2208
Bst4CI	ACNGT	8 cut(s)	172, 839, 1448, 2215, 2245, 2442, 2492, 2757
Bst6I	CTCTTC	5 cut(s)	612, 650, 1135, 1806, 2389
BstAUI	TGTACA	1 cut(s)	2554
BstC8I	GCNNGC	5 cut(s)	856, 1108, 1378, 2430, 2600
BstDEI	CTNAG	9 cut(s)	195, 301, 969, 1116, 1517, 1794, 2190, 2346, 2523
BstDSI	CCRYGG	3 cut(s)	339, 1188, 2280
BstEII	GGTNACC	1 cut(s)	2226
BstF5I	GGATG	9 cut(s)	121, 169, 241, 739, 1891, 1927, 2362, 2600, 2635
BstKTI	GATC	8 cut(s)	179, 566, 975, 1150, 1241, 1256, 2167, 2635
BstMAI	GTCTC	5 cut(s)	185, 930, 1454, 2248, 2519
BstMBI	GATC	8 cut(s)	176, 563, 972, 1147, 1238, 1253, 2164, 2632
BstMWI	GCNNNNNNNGC	6 cut(s)	264, 728, 1184, 1370, 1683, 1876
BstNI	CCWGG	4 cut(s)	744, 1750, 2006, 2208
BstNSI	RCATGY	4 cut(s)	1110, 1380, 1950, 2666
BstPI	GGTNACC	1 cut(s)	2226
BstSCI	CCNGG	4 cut(s)	742, 1748, 2004, 2206
BstSFI	CTRYAG	4 cut(s)	1227, 1365, 2511, 2756
BstV1I	GCAGC	4 cut(s)	613, 1299, 1357, 1360
BstV2I	GAAGAC	3 cut(s)	397, 2451, 2703
BstX2I	RGATCY	3 cut(s)	176, 1147, 1253
BstXI	CCANNNNNNTGG	4 cut(s)	187, 1266, 1494, 2647
BstYI	RGATCY	3 cut(s)	176, 1147, 1253
BsuI	GTATCC	1 cut(s)	2475
BsuRI	GGCC	6 cut(s)	65, 140, 318, 1831, 1903, 2610
BtgI	CCRYGG	3 cut(s)	339, 1188, 2280
BtrI	CACGTC	1 cut(s)	80
BtsCI	GGATG	9 cut(s)	121, 169, 241, 739, 1891, 1927, 2362, 2600, 2635
BtsI	GCAGTG	2 cut(s)	1630, 2120
BtsIMutI	CAGTG	6 cut(s)	269, 1514, 1572, 1630, 2120, 2745
BveI	ACCTGC	1 cut(s)	1431
Cac8I	GCNNGC	5 cut(s)	856, 1108, 1378, 2430, 2600
CaiI	CAGNNNCTG	4 cut(s)	824, 1446, 2213, 2455
CciI	TCATGA	2 cut(s)	349, 2656
Cfr13I	GGNCC	4 cut(s)	138, 317, 609, 1901
CseI	GACGC	1 cut(s)	1331
Csp6I	GTAC	6 cut(s)	710, 1264, 1279, 1444, 2039, 2555
CspCI	CAANNNNNGTGG	2 cut(s)	758, 793
CviQI	GTAC	6 cut(s)	710, 1264, 1279, 1444, 2039, 2555
DdeI	CTNAG	9 cut(s)	195, 301, 969, 1116, 1517, 1794, 2190, 2346, 2523
DpnI	GATC	8 cut(s)	178, 565, 974, 1149, 1240, 1255, 2166, 2634
DpnII	GATC	8 cut(s)	176, 563, 972, 1147, 1238, 1253, 2164, 2632
EaeI	YGGCCR	1 cut(s)	63
Eam1104I	CTCTTC	5 cut(s)	612, 650, 1135, 1806, 2389
EarI	CTCTTC	5 cut(s)	612, 650, 1135, 1806, 2389
EciI	GGCGGA	1 cut(s)	1174
Eco130I	CCWWGG	5 cut(s)	186, 339, 1940, 2231, 2280
Eco147I	AGGCCT	1 cut(s)	2610
Eco47I	GGWCC	1 cut(s)	609
Eco57I	CTGAAG	2 cut(s)	631, 1122
Eco91I	GGTNACC	1 cut(s)	2226
EcoO109I	RGGNCCY	1 cut(s)	609
EcoO65I	GGTNACC	1 cut(s)	2226
EcoRI	GAATTC	1 cut(s)	2294
EcoRII	CCWGG	4 cut(s)	742, 1748, 2004, 2206
EcoT14I	CCWWGG	5 cut(s)	186, 339, 1940, 2231, 2280
ErhI	CCWWGG	5 cut(s)	186, 339, 1940, 2231, 2280
FaqI	GGGAC	3 cut(s)	622, 2224, 2402
FauI	CCCGC	1 cut(s)	2284
FbaI	TGATCA	1 cut(s)	2164
Fnu4HI	GCNGC	4 cut(s)	627, 1313, 1371, 1374
FokI	GGATG	9 cut(s)	108, 176, 248, 726, 1878, 1914, 2369, 2607, 2642
Fsp4HI	GCNGC	4 cut(s)	627, 1313, 1371, 1374
FspBI	CTAG	9 cut(s)	231, 843, 903, 1674, 1679, 1782, 1941, 2258, 2549
GluI	GCNGC	4 cut(s)	627, 1313, 1371, 1374
GsaI	CCCAGC	2 cut(s)	689, 1416
GsuI	CTGGAG	1 cut(s)	1988
HaeIII	GGCC	6 cut(s)	65, 140, 318, 1831, 1903, 2610
HapII	CCGG	3 cut(s)	1127, 1923, 2627
HgaI	GACGC	1 cut(s)	1331
HincII	GTYRAC	1 cut(s)	1657
HindII	GTYRAC	1 cut(s)	1657
HindIII	AAGCTT	2 cut(s)	256, 2027
HpaII	CCGG	3 cut(s)	1127, 1923, 2627
HphI	GGTGA	6 cut(s)	53, 257, 703, 1116, 1543, 1838
Hpy166II	GTNNAC	5 cut(s)	1279, 1657, 1936, 2241, 2546
Hpy188III	TCNNGA	9 cut(s)	129, 350, 387, 602, 1151, 1242, 2068, 2657, 2671
Hpy8I	GTNNAC	5 cut(s)	1279, 1657, 1936, 2241, 2546
Hpy99I	CGWCG	1 cut(s)	411
HpyAV	CCTTC	5 cut(s)	599, 1244, 1348, 1676, 2210
HpyCH4III	ACNGT	8 cut(s)	172, 839, 1448, 2215, 2245, 2442, 2492, 2757
HpyCH4IV	ACGT	4 cut(s)	79, 400, 2017, 2175
HpyF10VI	GCNNNNNNNGC	6 cut(s)	264, 728, 1184, 1370, 1683, 1876
HpyF3I	CTNAG	9 cut(s)	195, 301, 969, 1116, 1517, 1794, 2190, 2346, 2523
HpySE526I	ACGT	4 cut(s)	79, 400, 2017, 2175
KflI	GGGWCCC	1 cut(s)	609
Ksp22I	TGATCA	1 cut(s)	2164
Kzo9I	GATC	8 cut(s)	176, 563, 972, 1147, 1238, 1253, 2164, 2632
LmnI	GCTCC	1 cut(s)	2278
Lsp1109I	GCAGC	4 cut(s)	613, 1299, 1357, 1360
MaeI	CTAG	9 cut(s)	231, 843, 903, 1674, 1679, 1782, 1941, 2258, 2549
MaeII	ACGT	4 cut(s)	79, 400, 2017, 2175
MaeIII	GTNAC	8 cut(s)	41, 355, 691, 1432, 2171, 2226, 2467, 2710
MalI	GATC	8 cut(s)	178, 565, 974, 1149, 1240, 1255, 2166, 2634
MboI	GATC	8 cut(s)	176, 563, 972, 1147, 1238, 1253, 2164, 2632
MfeI	CAATTG	1 cut(s)	2079
MflI	RGATCY	3 cut(s)	176, 1147, 1253
MhlI	GDGCHC	1 cut(s)	269
MlsI	TGGCCA	1 cut(s)	65
MluNI	TGGCCA	1 cut(s)	65
MlyI	GAGTC	3 cut(s)	141, 293, 1429
MmeI	TCCRAC	5 cut(s)	154, 1216, 1223, 1755, 1829
Mox20I	TGGCCA	1 cut(s)	65
MroXI	GAANNNNTTC	2 cut(s)	258, 444
MscI	TGGCCA	1 cut(s)	65
MseI	TTAA	4 cut(s)	470, 812, 1790, 2142
MslI	CAYNNNNRTG	2 cut(s)	1851, 2661
Msp20I	TGGCCA	1 cut(s)	65
MspA1I	CMGCKG	1 cut(s)	2522
MspI	CCGG	3 cut(s)	1127, 1923, 2627
MspR9I	CCNGG	4 cut(s)	744, 1750, 2006, 2208
MunI	CAATTG	1 cut(s)	2079
Mva1269I	GAATGC	2 cut(s)	769, 1669
MvaI	CCWGG	4 cut(s)	744, 1750, 2006, 2208
MwoI	GCNNNNNNNGC	6 cut(s)	264, 728, 1184, 1370, 1683, 1876
NcoI	CCATGG	2 cut(s)	339, 2280
NdeII	GATC	8 cut(s)	176, 563, 972, 1147, 1238, 1253, 2164, 2632
NlaIV	GGNNCC	8 cut(s)	178, 610, 611, 918, 1255, 1512, 1579, 2586
NmeAIII	GCCGAG	1 cut(s)	2452
NmuCI	GTSAC	4 cut(s)	41, 355, 691, 1432
NspI	RCATGY	4 cut(s)	1110, 1380, 1950, 2666
PaeI	GCATGC	2 cut(s)	1110, 1380
PagI	TCATGA	2 cut(s)	349, 2656
PceI	AGGCCT	1 cut(s)	2610
PciI	ACATGT	1 cut(s)	1946
PcsI	WCGNNNNNNNCGW	1 cut(s)	1196
PctI	GAATGC	2 cut(s)	769, 1669
PdmI	GAANNNNTTC	2 cut(s)	258, 444
PfeI	GAWTC	9 cut(s)	112, 383, 658, 1130, 1483, 1718, 1810, 1999, 2501
PfoI	TCCNGGA	1 cut(s)	2004
PkrI	GCNGC	4 cut(s)	628, 1314, 1372, 1375
PleI	GAGTC	3 cut(s)	140, 293, 1429
PpsI	GAGTC	3 cut(s)	140, 293, 1429
PpuMI	RGGWCCY	1 cut(s)	609
PscI	ACATGT	1 cut(s)	1946
PshBI	ATTAAT	1 cut(s)	2142
Psp1406I	AACGTT	1 cut(s)	2017
Psp5II	RGGWCCY	1 cut(s)	609
Psp6I	CCWGG	4 cut(s)	742, 1748, 2004, 2206
PspEI	GGTNACC	1 cut(s)	2226
PspFI	CCCAGC	2 cut(s)	685, 1412
PspGI	CCWGG	4 cut(s)	742, 1748, 2004, 2206
PspN4I	GGNNCC	8 cut(s)	178, 610, 611, 918, 1255, 1512, 1579, 2586
PspPI	GGNCC	4 cut(s)	138, 317, 609, 1901
PspPPI	RGGWCCY	1 cut(s)	609
PstNI	CAGNNNCTG	4 cut(s)	824, 1446, 2213, 2455
PsuI	RGATCY	3 cut(s)	176, 1147, 1253
PvuII	CAGCTG	1 cut(s)	2522
RsaI	GTAC	6 cut(s)	711, 1265, 1280, 1445, 2040, 2556
RsaNI	GTAC	6 cut(s)	710, 1264, 1279, 1444, 2039, 2555
RseI	CAYNNNNRTG	2 cut(s)	1851, 2661
SaqAI	TTAA	4 cut(s)	470, 812, 1790, 2142
SatI	GCNGC	4 cut(s)	627, 1313, 1371, 1374
Sau3AI	GATC	8 cut(s)	176, 563, 972, 1147, 1238, 1253, 2164, 2632
Sau96I	GGNCC	4 cut(s)	138, 317, 609, 1901
SchI	GAGTC	3 cut(s)	141, 293, 1429
ScrFI	CCNGG	4 cut(s)	744, 1750, 2006, 2208
SduI	GDGCHC	1 cut(s)	269
SfcI	CTRYAG	4 cut(s)	1227, 1365, 2511, 2756
SinI	GGWCC	1 cut(s)	609
SmiMI	CAYNNNNRTG	2 cut(s)	1851, 2661
SmlI	CTYRAG	3 cut(s)	1151, 1451, 1760
SmoI	CTYRAG	3 cut(s)	1151, 1451, 1760
SphI	GCATGC	2 cut(s)	1110, 1380
SseBI	AGGCCT	1 cut(s)	2610
SsiI	CCGC	3 cut(s)	1185, 1628, 2277
SspI	AATATT	2 cut(s)	876, 2364
SspMI	CTAG	9 cut(s)	231, 843, 903, 1674, 1679, 1782, 1941, 2258, 2549
StuI	AGGCCT	1 cut(s)	2610
StyD4I	CCNGG	4 cut(s)	742, 1748, 2004, 2206
StyI	CCWWGG	5 cut(s)	186, 339, 1940, 2231, 2280
TaaI	ACNGT	8 cut(s)	172, 839, 1448, 2215, 2245, 2442, 2492, 2757
TaiI	ACGT	4 cut(s)	82, 403, 2020, 2178
TaqI	TCGA	5 cut(s)	1241, 1359, 1727, 2091, 2643
TatI	WGTACW	5 cut(s)	709, 1263, 1278, 2038, 2554
TfiI	GAWTC	9 cut(s)	112, 383, 658, 1130, 1483, 1718, 1810, 1999, 2501
Tru1I	TTAA	4 cut(s)	470, 812, 1790, 2142
Tru9I	TTAA	4 cut(s)	470, 812, 1790, 2142
TscAI	CASTG	6 cut(s)	269, 1521, 1579, 1630, 2127, 2745
TseFI	GTSAC	4 cut(s)	41, 355, 691, 1432
TseI	GCWGC	4 cut(s)	626, 1312, 1370, 1373
Tsp45I	GTSAC	4 cut(s)	41, 355, 691, 1432
TspGWI	ACGGA	2 cut(s)	409, 527
TspRI	CASTG	6 cut(s)	269, 1521, 1579, 1630, 2127, 2745
VpaK11BI	GGWCC	1 cut(s)	609
VspI	ATTAAT	1 cut(s)	2142
XapI	RAATTY	4 cut(s)	334, 1602, 1636, 2294
XceI	RCATGY	4 cut(s)	1110, 1380, 1950, 2666
XcmI	CCANNNNNNNNNTGG	3 cut(s)	132, 746, 2141
XmaJI	CCTAGG	1 cut(s)	1940
XmnI	GAANNNNTTC	2 cut(s)	258, 444
XspI	CTAG	9 cut(s)	231, 843, 903, 1674, 1679, 1782, 1941, 2258, 2549

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.