FvH4_1g28900

EF-hand domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0006584
Physical Location & Seq
Forward (+)
21348367 .. 21349140
774 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g28900.t1

Sequence Viewer

Length: 258 bp
ATGGCTGAAGAGGATCCCAAAGAAAAGGCCGACCGTGAGCGCATTTTCAAGCACTTCGACACCAATGGCGACGGCAAAATCTCGGCAGATGAGCTAGGGGATGCATTGAAAGCTCTCGGATGCGTTTCAACGGAAGAAGTGAAATTCATGATGGAAGAGATTGACACCGACCGTGACGGCTTCATTTCTTATGAGGAGTATTTATCTTTTGCTCTTGCCAACCGTGGTCTAATGAAGGACATTGCCAAGGTATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1

Protein Analysis

86

Amino Acids

9.64

Weight (kDa)

4.49

Isoelectric Point (pI)

13.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 13 - 39 6.4e-09 EF hand domain
AIF-1 PF21008 13 - 77 8.6e-06 Allograft inflammatory factor 1
EF-hand_7 PF13499 14 - 71 8.4e-17 EF-hand domain pair
EF-hand_6 PF13405 14 - 40 2.3e-09 EF-hand domain
EF-hand_5 PF13202 15 - 33 8.8e-07 EF hand
EF-hand_8 PF13833 26 - 71 1.3e-06 EF-hand domain pair
EF-hand_1 PF00036 46 - 70 3.9e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 8, 21
AcsI RAATTY 1 cut(s) 143
AcuI CTGAAG 1 cut(s) 27
AgsI TTSAA 3 cut(s) 49, 109, 129
AluBI AGCT 2 cut(s) 94, 113
AluI AGCT 2 cut(s) 94, 113
AlwI GGATC 2 cut(s) 8, 21
AoxI GGCC 1 cut(s) 27
ApoI RAATTY 1 cut(s) 143
AspLEI GCGC 1 cut(s) 42
BamHI GGATCC 1 cut(s) 13
BccI CCATC 1 cut(s) 145
BceAI ACGGC 2 cut(s) 88, 193
BfaI CTAG 1 cut(s) 95
BmiI GGNNCC 1 cut(s) 15
BmsI GCATC 2 cut(s) 91, 110
BsaJI CCNNGG 2 cut(s) 223, 246
Bse3DI GCAATG 1 cut(s) 240
BseDI CCNNGG 2 cut(s) 223, 246
BseGI GGATG 2 cut(s) 106, 125
BseMI GCAATG 1 cut(s) 240
BseRI GAGGAG 1 cut(s) 209
Bsh1285I CGRYCG 2 cut(s) 34, 172
BshFI GGCC 1 cut(s) 29
BsiEI CGRYCG 2 cut(s) 34, 172
BsnI GGCC 1 cut(s) 29
Bsp143I GATC 1 cut(s) 13
BspANI GGCC 1 cut(s) 29
BspHI TCATGA 1 cut(s) 147
BspLI GGNNCC 1 cut(s) 15
BspPI GGATC 2 cut(s) 8, 21
BsrDI GCAATG 1 cut(s) 240
BssECI CCNNGG 2 cut(s) 223, 246
BssMI GATC 1 cut(s) 13
BssT1I CCWWGG 1 cut(s) 246
Bst4CI ACNGT 3 cut(s) 35, 173, 224
Bst6I CTCTTC 2 cut(s) 3, 150
BstDSI CCRYGG 1 cut(s) 223
BstF5I GGATG 2 cut(s) 106, 125
BstHHI GCGC 1 cut(s) 42
BstKTI GATC 1 cut(s) 16
BstMBI GATC 1 cut(s) 13
BstMCI CGRYCG 2 cut(s) 34, 172
BstMWI GCNNNNNNNGC 1 cut(s) 110
BstX2I RGATCY 1 cut(s) 13
BstYI RGATCY 1 cut(s) 13
BsuRI GGCC 1 cut(s) 29
BtgI CCRYGG 1 cut(s) 223
BtsCI GGATG 2 cut(s) 106, 125
CciI TCATGA 1 cut(s) 147
CfoI GCGC 1 cut(s) 42
CviAII CATG 1 cut(s) 148
CviJI RGCY 5 cut(s) 5, 29, 94, 113, 180
CviKI_1 RGCY 5 cut(s) 5, 29, 94, 113, 180
DpnI GATC 1 cut(s) 15
DpnII GATC 1 cut(s) 13
Eam1104I CTCTTC 2 cut(s) 3, 150
EarI CTCTTC 2 cut(s) 3, 150
Eco130I CCWWGG 1 cut(s) 246
Eco57I CTGAAG 1 cut(s) 27
EcoT14I CCWWGG 1 cut(s) 246
EcoT22I ATGCAT 1 cut(s) 106
ErhI CCWWGG 1 cut(s) 246
FaeI CATG 1 cut(s) 151
FaiI YATR 2 cut(s) 149, 192
FatI CATG 1 cut(s) 147
FokI GGATG 2 cut(s) 113, 132
FspBI CTAG 1 cut(s) 95
GlaI GCGC 1 cut(s) 41
HaeIII GGCC 1 cut(s) 29
HhaI GCGC 1 cut(s) 42
Hin1II CATG 1 cut(s) 151
Hin6I GCGC 1 cut(s) 40
HinP1I GCGC 1 cut(s) 40
Hpy188I TCNGA 1 cut(s) 119
Hpy188III TCNNGA 1 cut(s) 148
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 1 cut(s) 229
HpyCH4III ACNGT 3 cut(s) 35, 173, 224
HpyCH4V TGCA 1 cut(s) 104
HpyF10VI GCNNNNNNNGC 1 cut(s) 110
Hsp92II CATG 1 cut(s) 151
HspAI GCGC 1 cut(s) 40
Kzo9I GATC 1 cut(s) 13
LweI GCATC 2 cut(s) 91, 110
MaeI CTAG 1 cut(s) 95
MaeIII GTNAC 1 cut(s) 173
MalI GATC 1 cut(s) 15
MboI GATC 1 cut(s) 13
MboII GAAGA 3 cut(s) 20, 146, 167
MflI RGATCY 1 cut(s) 13
MluCI AATT 1 cut(s) 143
MnlI CCTC 2 cut(s) 4, 187
Mph1103I ATGCAT 1 cut(s) 106
MseI TTAA 1 cut(s) 256
MwoI GCNNNNNNNGC 1 cut(s) 110
NdeII GATC 1 cut(s) 13
NlaIII CATG 1 cut(s) 151
NlaIV GGNNCC 1 cut(s) 15
NmeAIII GCCGAG 1 cut(s) 62
NmuCI GTSAC 1 cut(s) 173
NsiI ATGCAT 1 cut(s) 106
PagI TCATGA 1 cut(s) 147
PspN4I GGNNCC 1 cut(s) 15
PsuI RGATCY 1 cut(s) 13
SaqAI TTAA 1 cut(s) 256
Sau3AI GATC 1 cut(s) 13
SetI ASST 3 cut(s) 96, 115, 252
SfaNI GCATC 2 cut(s) 91, 110
SgeI CNNG 9 cut(s) 47, 61, 94, 107, 128, 160, 185, 227, 236
Sse9I AATT 1 cut(s) 143
SspMI CTAG 1 cut(s) 95
StyI CCWWGG 1 cut(s) 246
TaaI ACNGT 3 cut(s) 35, 173, 224
TaqI TCGA 1 cut(s) 57
TasI AATT 1 cut(s) 143
Tru1I TTAA 1 cut(s) 256
Tru9I TTAA 1 cut(s) 256
TseFI GTSAC 1 cut(s) 173
Tsp45I GTSAC 1 cut(s) 173
TspDTI ATGAA 3 cut(s) 136, 172, 248
TspGWI ACGGA 1 cut(s) 146
XapI RAATTY 1 cut(s) 143
XspI CTAG 1 cut(s) 95
Zsp2I ATGCAT 1 cut(s) 106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.