Rorug03G0260800

EF-hand domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0006584
Physical Location & Seq
Forward (+)
25089521 .. 25090315
795 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0260800.1

Sequence Viewer

Length: 795 bp
ATGGAAGCGGAAGACGACGAATTCGAAAACGCCTACAACGCCGCCTCTTCGCCGCCGCGGAGGCCGAGGTCCATCTGCGGCGACTGCAGCCGGCCCGGCCCAGTCTGCCTCTGCAAGTCTCTCCCGGCCCAGCCCATCCCAACCCAAACCCAAATCATAATCCTTCACCATCCTCACGAGGCGCAGCACAAGCTCTCCACCACGCCGGTCCTGACAAAATGCCTGGCCAACTCCACCGCCATCGTCAGCCGCCGCCTCAAGCCGGGCCTGTCCCCGCTCCTCGACCAATCACCTTCCGCCATCTACCTCTTCCCGCCCTCGAAATCCTCACCGTCCGTCAGTTTATCCCGTCTCCGGATCCCCGACCAGTCGGCGCCGGTGGTGGTCGTAGCTTTCGACGCGACGTGGAAGCACGCGAAGGAGATGGTGAGGGCGAGCGAGGGGTTCCTGTCGAAATTCGCGAGGAGGGTGAGTTTGGATGTGGATGAGGATGTGAGGGGAGGGAGCATCTACGACTCGGAGTTGATTCTGAGGAAGGAGCCGTTTGGTGGGTGCGTGAGTACCATGGAGGCCGTGGCCAGGGCTCTGAGAGTGCTCGAGCCCAATGGGTTGGAGATTGAGAGCAAGCTTATTCGGGTTTTGAGGGACATGGTGGCGCTGCAGGCGGGTTTTCTTAAGCCCGTGAAGCACAGGCCCAAGTTGTTGAAGAAAGGGAAGGAGGAACAGAGGCTCAAGGATGAGAGTAGTGAGAGTTCCCCGATAGTTGGTGAAGGTCAGTCACTCAGCTTTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1

Protein Analysis

264

Amino Acids

28.91

Weight (kDa)

8.88

Isoelectric Point (pI)

63.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DTW PF03942 23 - 221 4.9e-39 DTW domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 373
AccBSI CCGCTC 1 cut(s) 277
AccII CGCG 4 cut(s) 58, 401, 416, 461
AccIII TCCGGA 1 cut(s) 354
AclWI GGATC 2 cut(s) 352, 365
AcoI YGGCCR 2 cut(s) 225, 576
AcsI RAATTY 2 cut(s) 20, 455
AcyI GRCGYC 1 cut(s) 374
AfaI GTAC 1 cut(s) 562
AfiI CCNNNNNNNGG 5 cut(s) 262, 354, 548, 579, 764
AflII CTTAAG 1 cut(s) 674
AgsI TTSAA 1 cut(s) 706
AjiI CACGTC 1 cut(s) 405
AjnI CCWGG 2 cut(s) 222, 578
AluBI AGCT 4 cut(s) 193, 392, 628, 786
AluI AGCT 4 cut(s) 193, 392, 628, 786
Alw21I GWGCWC 1 cut(s) 597
Alw26I GTCTC 2 cut(s) 123, 356
AlwI GGATC 2 cut(s) 352, 365
Ama87I CYCGRG 1 cut(s) 596
Aor13HI TCCGGA 1 cut(s) 354
AoxI GGCC 9 cut(s) 62, 92, 97, 126, 225, 265, 570, 576, 692
ApeKI GCWGC 3 cut(s) 87, 184, 658
ApoI RAATTY 2 cut(s) 20, 455
ArsI GACNNNNNNTTYG 1 cut(s) 37
AspLEI GCGC 3 cut(s) 184, 376, 658
AspS9I GGNCC 7 cut(s) 69, 93, 98, 127, 208, 265, 693
AsuC2I CCSGG 3 cut(s) 96, 125, 264
AsuHPI GGTGA 6 cut(s) 158, 282, 321, 439, 481, 779
AsuII TTCGAA 1 cut(s) 24
AvaI CYCGRG 1 cut(s) 596
AvaII GGWCC 2 cut(s) 69, 208
BalI TGGCCA 2 cut(s) 227, 578
BamHI GGATCC 1 cut(s) 357
BanI GGYRCC 1 cut(s) 373
BanII GRGCYC 2 cut(s) 586, 603
BauI CACGAG 1 cut(s) 176
BbsI GAAGAC 1 cut(s) 18
Bbv12I GWGCWC 1 cut(s) 597
BbvI GCAGC 3 cut(s) 99, 196, 645
BccI CCATC 6 cut(s) 80, 143, 177, 248, 308, 418
BceAI ACGGC 2 cut(s) 526, 557
BciT130I CCWGG 2 cut(s) 224, 580
BcnI CCSGG 3 cut(s) 96, 125, 264
BcoDI GTCTC 2 cut(s) 123, 356
BfmI CTRYAG 2 cut(s) 85, 659
BfoI RGCGCY 2 cut(s) 377, 659
BfrI CTTAAG 1 cut(s) 674
BglI GCCNNNNNGGC 2 cut(s) 61, 96
BisI GCNGC 9 cut(s) 42, 53, 56, 79, 88, 185, 250, 253, 659
BlsI GCNGC 9 cut(s) 43, 54, 57, 80, 89, 186, 251, 254, 660
Bme1390I CCNGG 5 cut(s) 96, 125, 224, 264, 580
Bme18I GGWCC 2 cut(s) 69, 208
BmeT110I CYCGRG 1 cut(s) 596
BmgBI CACGTC 1 cut(s) 405
BmgT120I GGNCC 7 cut(s) 69, 93, 98, 127, 208, 265, 693
BmiI GGNNCC 4 cut(s) 359, 375, 446, 540
BmrFI CCNGG 5 cut(s) 96, 125, 224, 264, 580
BmrI ACTGGG 1 cut(s) 95
BmsI GCATC 1 cut(s) 516
BmuI ACTGGG 1 cut(s) 95
BpiI GAAGAC 1 cut(s) 18
Bpu14I TTCGAA 1 cut(s) 24
BpuEI CTTGAG 2 cut(s) 242, 716
BpuMI CCSGG 3 cut(s) 96, 125, 264
BsaHI GRCGYC 1 cut(s) 374
BsaJI CCNNGG 5 cut(s) 56, 65, 564, 573, 579
BsaWI WCCGGW 1 cut(s) 354
BsaXI ACNNNNNCTCC 4 cut(s) 179, 209, 457, 487
Bsc4I CCNNNNNNNGG 5 cut(s) 262, 354, 548, 579, 764
Bse118I RCCGGY 3 cut(s) 90, 205, 376
Bse1I ACTGG 2 cut(s) 101, 367
BseAI TCCGGA 1 cut(s) 354
BseBI CCWGG 2 cut(s) 224, 580
BseDI CCNNGG 5 cut(s) 56, 65, 564, 573, 579
BseGI GGATG 6 cut(s) 135, 169, 484, 490, 496, 742
BseLI CCNNNNNNNGG 5 cut(s) 262, 354, 548, 579, 764
BseMII CTCAG 2 cut(s) 521, 578
BseNI ACTGG 2 cut(s) 101, 367
BseRI GAGGAG 2 cut(s) 269, 478
BseXI GCAGC 3 cut(s) 99, 196, 645
BseYI CCCAGC 1 cut(s) 129
Bsh1236I CGCG 4 cut(s) 58, 401, 416, 461
BshFI GGCC 9 cut(s) 64, 94, 99, 128, 227, 267, 572, 578, 694
BshNI GGYRCC 1 cut(s) 373
BsiHKAI GWGCWC 1 cut(s) 597
BsiHKCI CYCGRG 1 cut(s) 596
BsiSI CCGG 7 cut(s) 91, 96, 125, 206, 263, 355, 377
BslFI GGGAC 2 cut(s) 256, 659
BslI CCNNNNNNNGG 5 cut(s) 262, 354, 548, 579, 764
BsmAI GTCTC 2 cut(s) 123, 356
BsmBI CGTCTC 1 cut(s) 356
BsmFI GGGAC 2 cut(s) 256, 659
BsnI GGCC 9 cut(s) 64, 94, 99, 128, 227, 267, 572, 578, 694
BsoBI CYCGRG 1 cut(s) 596
Bsp119I TTCGAA 1 cut(s) 24
Bsp1286I GDGCHC 3 cut(s) 586, 597, 603
Bsp13I TCCGGA 1 cut(s) 354
Bsp143I GATC 1 cut(s) 357
Bsp19I CCATGG 1 cut(s) 564
Bsp68I TCGCGA 1 cut(s) 461
BspANI GGCC 9 cut(s) 64, 94, 99, 128, 227, 267, 572, 578, 694
BspCNI CTCAG 3 cut(s) 522, 579, 795
BspEI TCCGGA 1 cut(s) 354
BspFNI CGCG 4 cut(s) 58, 401, 416, 461
BspLI GGNNCC 4 cut(s) 359, 375, 446, 540
BspMAI CTGCAG 2 cut(s) 89, 663
BspPI GGATC 2 cut(s) 352, 365
BspT104I TTCGAA 1 cut(s) 24
BspT107I GGYRCC 1 cut(s) 373
BspTI CTTAAG 1 cut(s) 674
BsrBI CCGCTC 1 cut(s) 277
BsrFI RCCGGY 3 cut(s) 90, 205, 376
BsrI ACTGG 2 cut(s) 101, 367
BssAI RCCGGY 3 cut(s) 90, 205, 376
BssECI CCNNGG 5 cut(s) 56, 65, 564, 573, 579
BssMI GATC 1 cut(s) 357
BssNI GRCGYC 1 cut(s) 374
BssSI CACGAG 1 cut(s) 176
BssT1I CCWWGG 1 cut(s) 564
Bst2BI CACGAG 1 cut(s) 176
Bst2UI CCWGG 2 cut(s) 224, 580
Bst4CI ACNGT 1 cut(s) 333
Bst6I CTCTTC 2 cut(s) 52, 314
BstACI GRCGYC 1 cut(s) 374
BstAFI CTTAAG 1 cut(s) 674
BstBI TTCGAA 1 cut(s) 24
BstC8I GCNNGC 5 cut(s) 92, 414, 436, 626, 663
BstDEI CTNAG 3 cut(s) 530, 587, 782
BstDSI CCRYGG 3 cut(s) 56, 564, 573
BstF5I GGATG 6 cut(s) 135, 169, 484, 490, 496, 742
BstFNI CGCG 4 cut(s) 58, 401, 416, 461
BstH2I RGCGCY 2 cut(s) 377, 659
BstHHI GCGC 3 cut(s) 184, 376, 658
BstKTI GATC 1 cut(s) 360
BstMAI GTCTC 2 cut(s) 123, 356
BstMBI GATC 1 cut(s) 357
BstNI CCWGG 2 cut(s) 224, 580
BstSCI CCNGG 5 cut(s) 94, 123, 222, 262, 578
BstSFI CTRYAG 2 cut(s) 85, 659
BstUI CGCG 4 cut(s) 58, 401, 416, 461
BstV1I GCAGC 3 cut(s) 99, 196, 645
BstV2I GAAGAC 1 cut(s) 18
BstX2I RGATCY 1 cut(s) 357
BstXI CCANNNNNNTGG 1 cut(s) 610
BstYI RGATCY 1 cut(s) 357
BsuRI GGCC 9 cut(s) 64, 94, 99, 128, 227, 267, 572, 578, 694
BtgI CCRYGG 3 cut(s) 56, 564, 573
BtrI CACGTC 1 cut(s) 405
BtsCI GGATG 6 cut(s) 135, 169, 484, 490, 496, 742
BtuMI TCGCGA 1 cut(s) 461
Cac8I GCNNGC 5 cut(s) 92, 414, 436, 626, 663
CfoI GCGC 3 cut(s) 184, 376, 658
Cfr10I RCCGGY 3 cut(s) 90, 205, 376
Cfr13I GGNCC 7 cut(s) 69, 93, 98, 127, 208, 265, 693
Cfr42I CCGCGG 1 cut(s) 59
CseI GACGC 1 cut(s) 407
Csp6I GTAC 1 cut(s) 561
CviAII CATG 2 cut(s) 565, 649
CviQI GTAC 1 cut(s) 561
DdeI CTNAG 3 cut(s) 530, 587, 782
DinI GGCGCC 1 cut(s) 375
DpnI GATC 1 cut(s) 359
DpnII GATC 1 cut(s) 357
EaeI YGGCCR 2 cut(s) 225, 576
Eam1104I CTCTTC 2 cut(s) 52, 314
EarI CTCTTC 2 cut(s) 52, 314
EciI GGCGGA 1 cut(s) 286
Eco130I CCWWGG 1 cut(s) 564
Eco24I GRGCYC 2 cut(s) 586, 603
Eco47I GGWCC 2 cut(s) 69, 208
Eco88I CYCGRG 1 cut(s) 596
EcoRI GAATTC 1 cut(s) 20
EcoRII CCWGG 2 cut(s) 222, 578
EcoT14I CCWWGG 1 cut(s) 564
EcoT38I GRGCYC 2 cut(s) 586, 603
EgeI GGCGCC 1 cut(s) 375
EheI GGCGCC 1 cut(s) 375
ErhI CCWWGG 1 cut(s) 564
Esp3I CGTCTC 1 cut(s) 356
FaeI CATG 2 cut(s) 568, 652
FaiI YATR 3 cut(s) 158, 566, 650
FaqI GGGAC 2 cut(s) 256, 659
FatI CATG 2 cut(s) 564, 648
FauI CCCGC 3 cut(s) 282, 321, 658
Fnu4HI GCNGC 9 cut(s) 42, 53, 56, 79, 88, 185, 250, 253, 659
FokI GGATG 6 cut(s) 122, 156, 491, 497, 503, 749
FriOI GRGCYC 2 cut(s) 586, 603
Fsp4HI GCNGC 9 cut(s) 42, 53, 56, 79, 88, 185, 250, 253, 659
GlaI GCGC 3 cut(s) 183, 375, 657
GluI GCNGC 9 cut(s) 42, 53, 56, 79, 88, 185, 250, 253, 659
GsaI CCCAGC 1 cut(s) 133
HaeII RGCGCY 2 cut(s) 377, 659
HaeIII GGCC 9 cut(s) 64, 94, 99, 128, 227, 267, 572, 578, 694
HapII CCGG 7 cut(s) 91, 96, 125, 206, 263, 355, 377
HgaI GACGC 1 cut(s) 407
HhaI GCGC 3 cut(s) 184, 376, 658
Hin1I GRCGYC 1 cut(s) 374
Hin1II CATG 2 cut(s) 568, 652
Hin6I GCGC 3 cut(s) 182, 374, 656
HinP1I GCGC 3 cut(s) 182, 374, 656
HindIII AAGCTT 1 cut(s) 626
HinfI GANTC 2 cut(s) 515, 526
HpaII CCGG 7 cut(s) 91, 96, 125, 206, 263, 355, 377
HphI GGTGA 6 cut(s) 158, 282, 321, 439, 481, 779
Hpy188I TCNGA 3 cut(s) 520, 531, 588
Hpy188III TCNNGA 4 cut(s) 176, 211, 355, 460
Hpy99I CGWCG 3 cut(s) 20, 401, 406
HpyAV CCTTC 6 cut(s) 173, 303, 412, 529, 709, 764
HpyCH4III ACNGT 1 cut(s) 333
HpyCH4IV ACGT 1 cut(s) 404
HpyCH4V TGCA 3 cut(s) 87, 114, 661
HpyF3I CTNAG 3 cut(s) 530, 587, 782
HpySE526I ACGT 1 cut(s) 404
Hsp92I GRCGYC 1 cut(s) 374
Hsp92II CATG 2 cut(s) 568, 652
HspAI GCGC 3 cut(s) 182, 374, 656
KasI GGCGCC 1 cut(s) 373
Kpn2I TCCGGA 1 cut(s) 354
KroI GCCGGC 1 cut(s) 90
KroNI GCCGGC 1 cut(s) 92
KspI CCGCGG 1 cut(s) 59
Kzo9I GATC 1 cut(s) 357
LmnI GCTCC 3 cut(s) 282, 504, 538
Lsp1109I GCAGC 3 cut(s) 99, 196, 645
LweI GCATC 1 cut(s) 516
MaeII ACGT 1 cut(s) 404
MaeIII GTNAC 1 cut(s) 777
MalI GATC 1 cut(s) 359
MbiI CCGCTC 1 cut(s) 277
MboI GATC 1 cut(s) 357
MboII GAAGA 4 cut(s) 23, 39, 301, 718
MflI RGATCY 1 cut(s) 357
MhlI GDGCHC 3 cut(s) 586, 597, 603
MlsI TGGCCA 2 cut(s) 227, 578
MluCI AATT 2 cut(s) 20, 455
MluNI TGGCCA 2 cut(s) 227, 578
Mly113I GGCGCC 1 cut(s) 374
MlyI GAGTC 1 cut(s) 509
MmeI TCCRAC 1 cut(s) 591
Mox20I TGGCCA 2 cut(s) 227, 578
MroI TCCGGA 1 cut(s) 354
MroNI GCCGGC 1 cut(s) 90
MscI TGGCCA 2 cut(s) 227, 578
MseI TTAA 1 cut(s) 675
Msp20I TGGCCA 2 cut(s) 227, 578
MspA1I CMGCKG 1 cut(s) 58
MspCI CTTAAG 1 cut(s) 674
MspI CCGG 7 cut(s) 91, 96, 125, 206, 263, 355, 377
MspR9I CCNGG 5 cut(s) 96, 125, 224, 264, 580
MvaI CCWGG 2 cut(s) 224, 580
MvnI CGCG 4 cut(s) 58, 401, 416, 461
NaeI GCCGGC 1 cut(s) 92
NarI GGCGCC 1 cut(s) 374
NciI CCSGG 3 cut(s) 96, 125, 264
NcoI CCATGG 1 cut(s) 564
NdeII GATC 1 cut(s) 357
NgoMIV GCCGGC 1 cut(s) 90
NlaIII CATG 2 cut(s) 568, 652
NlaIV GGNNCC 4 cut(s) 359, 375, 446, 540
NmeAIII GCCGAG 1 cut(s) 90
NmuCI GTSAC 1 cut(s) 777
NruI TCGCGA 1 cut(s) 461
NspV TTCGAA 1 cut(s) 24
PaeR7I CTCGAG 1 cut(s) 596
PcsI WCGNNNNNNNCGW 3 cut(s) 21, 393, 458
PdiI GCCGGC 1 cut(s) 92
PfeI GAWTC 1 cut(s) 526
PkrI GCNGC 9 cut(s) 43, 54, 57, 80, 89, 186, 251, 254, 660
PleI GAGTC 1 cut(s) 509
PluTI GGCGCC 1 cut(s) 377
PpsI GAGTC 1 cut(s) 509
Psp6I CCWGG 2 cut(s) 222, 578
PspFI CCCAGC 1 cut(s) 129
PspGI CCWGG 2 cut(s) 222, 578
PspN4I GGNNCC 4 cut(s) 359, 375, 446, 540
PspPI GGNCC 7 cut(s) 69, 93, 98, 127, 208, 265, 693
PspXI VCTCGAGB 1 cut(s) 596
PsrI GAACNNNNNNTAC 2 cut(s) 736, 768
PstI CTGCAG 2 cut(s) 89, 663
PsuI RGATCY 1 cut(s) 357
RruI TCGCGA 1 cut(s) 461
RsaI GTAC 1 cut(s) 562
RsaNI GTAC 1 cut(s) 561
SacII CCGCGG 1 cut(s) 59
SaqAI TTAA 1 cut(s) 675
SatI GCNGC 9 cut(s) 42, 53, 56, 79, 88, 185, 250, 253, 659
Sau3AI GATC 1 cut(s) 357
Sau96I GGNCC 7 cut(s) 69, 93, 98, 127, 208, 265, 693
SchI GAGTC 1 cut(s) 509
ScrFI CCNGG 5 cut(s) 96, 125, 224, 264, 580
SduI GDGCHC 3 cut(s) 586, 597, 603
SetI ASST 9 cut(s) 71, 195, 295, 309, 394, 407, 630, 775, 788
SfaNI GCATC 1 cut(s) 516
SfcI CTRYAG 2 cut(s) 85, 659
SfoI GGCGCC 1 cut(s) 375
Sfr274I CTCGAG 1 cut(s) 596
Sfr303I CCGCGG 1 cut(s) 59
SfuI TTCGAA 1 cut(s) 24
SgrAI CRCCGGYG 1 cut(s) 376
SgrBI CCGCGG 1 cut(s) 59
SinI GGWCC 2 cut(s) 69, 208
SlaI CTCGAG 1 cut(s) 596
SmlI CTYRAG 4 cut(s) 257, 596, 674, 731
SmoI CTYRAG 4 cut(s) 257, 596, 674, 731
Sse9I AATT 2 cut(s) 20, 455
SspDI GGCGCC 1 cut(s) 373
StyD4I CCNGG 5 cut(s) 94, 123, 222, 262, 578
StyI CCWWGG 1 cut(s) 564
TaaI ACNGT 1 cut(s) 333
TaiI ACGT 1 cut(s) 407
TaqI TCGA 6 cut(s) 24, 282, 320, 396, 452, 597
TasI AATT 2 cut(s) 20, 455
TauI GCSGC 6 cut(s) 44, 55, 58, 81, 252, 255
TfiI GAWTC 1 cut(s) 526
Tru1I TTAA 1 cut(s) 675
Tru9I TTAA 1 cut(s) 675
TseFI GTSAC 1 cut(s) 777
TseI GCWGC 3 cut(s) 87, 184, 658
Tsp45I GTSAC 1 cut(s) 777
TspGWI ACGGA 1 cut(s) 325
Vha464I CTTAAG 1 cut(s) 674
VpaK11BI GGWCC 2 cut(s) 69, 208
XapI RAATTY 2 cut(s) 20, 455
XcmI CCANNNNNNNNNTGG 1 cut(s) 571
XhoI CTCGAG 1 cut(s) 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.