RLG00000032434

Polcalcin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0006584
Physical Location & Seq
Forward (+)
15121021 .. 15121776
756 bp
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UTR
Exon/CDS
Intron
RLM00000032434

Sequence Viewer

Length: 345 bp
ATGGCTGATGATGCCGACGCTAAGGCTGGATGTGAGCGTATATTTAAACGCTTTGATTACAATGGTGATGCCAAAGTCTCACTGACCGAGTTCGCGGATGCACTTAAAGCACTCGGCACTTCTTCGCCGGAGGAAGTGCGTCAACGAATGGACGAGATCGATATGGATCATGATGGATTCATTTCACTGGAGGAGCTCTTCGCCTTCCAACGTGCCAACCCTGATTTAATGAAGGAAGTCATCAAGAAACTTTCACCGACCAAGGAGAAGGCAATTAAAAAGACTGCCTCAACTTTGCCAACTATATACATAAACCTTTCGAGGTCATTCATACACAACCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5

Protein Analysis

115

Amino Acids

12.9

Weight (kDa)

5.6

Isoelectric Point (pI)

36.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 11 - 69 7.8e-11 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 95
AciI CCGC 1 cut(s) 95
AclWI GGATC 1 cut(s) 174
AluBI AGCT 1 cut(s) 196
AluI AGCT 1 cut(s) 196
Alw21I GWGCWC 1 cut(s) 198
Alw26I GTCTC 1 cut(s) 82
AlwI GGATC 1 cut(s) 174
AsuHPI GGTGA 2 cut(s) 77, 246
BanII GRGCYC 1 cut(s) 198
Bbv12I GWGCWC 1 cut(s) 198
BccI CCATC 1 cut(s) 167
BcoDI GTCTC 1 cut(s) 82
BmsI GCATC 2 cut(s) 58, 88
BpmI CTGGAG 1 cut(s) 209
Bpu10I CCTNAGC 1 cut(s) 21
Bsa29I ATCGAT 1 cut(s) 159
BsaBI GATNNNNATC 1 cut(s) 165
BsaJI CCNNGG 1 cut(s) 261
Bse1I ACTGG 1 cut(s) 192
Bse8I GATNNNNATC 1 cut(s) 165
BseCI ATCGAT 1 cut(s) 159
BseDI CCNNGG 1 cut(s) 261
BseGI GGATG 2 cut(s) 35, 103
BseJI GATNNNNATC 1 cut(s) 165
BseNI ACTGG 1 cut(s) 192
BseRI GAGGAG 1 cut(s) 206
Bsh1236I CGCG 1 cut(s) 95
BshVI ATCGAT 1 cut(s) 159
BsiHKAI GWGCWC 1 cut(s) 198
BsiSI CCGG 1 cut(s) 128
BsmAI GTCTC 1 cut(s) 82
Bsp1286I GDGCHC 1 cut(s) 198
Bsp143I GATC 2 cut(s) 156, 166
BspACI CCGC 1 cut(s) 95
BspDI ATCGAT 1 cut(s) 159
BspFNI CGCG 1 cut(s) 95
BspHI TCATGA 1 cut(s) 169
BspPI GGATC 1 cut(s) 174
BspQI GCTCTTC 1 cut(s) 203
BsrI ACTGG 1 cut(s) 192
BssECI CCNNGG 1 cut(s) 261
BssMI GATC 2 cut(s) 156, 166
BssT1I CCWWGG 1 cut(s) 261
Bst6I CTCTTC 1 cut(s) 203
BstDEI CTNAG 1 cut(s) 21
BstF5I GGATG 2 cut(s) 35, 103
BstFNI CGCG 1 cut(s) 95
BstKTI GATC 2 cut(s) 159, 169
BstMAI GTCTC 1 cut(s) 82
BstMBI GATC 2 cut(s) 156, 166
BstMWI GCNNNNNNNGC 2 cut(s) 11, 107
BstUI CGCG 1 cut(s) 95
Bsu15I ATCGAT 1 cut(s) 159
BsuTUI ATCGAT 1 cut(s) 159
BtsCI GGATG 2 cut(s) 35, 103
BtsIMutI CAGTG 2 cut(s) 80, 185
CciI TCATGA 1 cut(s) 169
ClaI ATCGAT 1 cut(s) 159
CseI GACGC 2 cut(s) 26, 128
CviAII CATG 1 cut(s) 170
CviJI RGCY 3 cut(s) 5, 26, 196
CviKI_1 RGCY 3 cut(s) 5, 26, 196
DdeI CTNAG 1 cut(s) 21
DpnI GATC 2 cut(s) 158, 168
DpnII GATC 2 cut(s) 156, 166
DraI TTTAAA 1 cut(s) 46
Eam1104I CTCTTC 1 cut(s) 203
EarI CTCTTC 1 cut(s) 203
Ecl136II GAGCTC 1 cut(s) 196
Eco130I CCWWGG 1 cut(s) 261
Eco24I GRGCYC 1 cut(s) 198
Eco53kI GAGCTC 1 cut(s) 196
EcoICRI GAGCTC 1 cut(s) 196
EcoT14I CCWWGG 1 cut(s) 261
EcoT38I GRGCYC 1 cut(s) 198
ErhI CCWWGG 1 cut(s) 261
FaeI CATG 1 cut(s) 173
FaiI YATR 7 cut(s) 41, 164, 171, 305, 307, 311, 332
FatI CATG 1 cut(s) 169
FokI GGATG 2 cut(s) 42, 110
FriOI GRGCYC 1 cut(s) 198
GsuI CTGGAG 1 cut(s) 209
HapII CCGG 1 cut(s) 128
HgaI GACGC 2 cut(s) 26, 128
Hin1II CATG 1 cut(s) 173
HincII GTYRAC 1 cut(s) 143
HindII GTYRAC 1 cut(s) 143
HinfI GANTC 1 cut(s) 177
HpaII CCGG 1 cut(s) 128
HphI GGTGA 2 cut(s) 77, 246
Hpy166II GTNNAC 1 cut(s) 143
Hpy188III TCNNGA 2 cut(s) 170, 244
Hpy8I GTNNAC 1 cut(s) 143
Hpy99I CGWCG 1 cut(s) 20
HpyAV CCTTC 3 cut(s) 214, 226, 262
HpyCH4IV ACGT 1 cut(s) 211
HpyCH4V TGCA 1 cut(s) 101
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 107
HpyF3I CTNAG 1 cut(s) 21
HpySE526I ACGT 1 cut(s) 211
Hsp92II CATG 1 cut(s) 173
Kzo9I GATC 2 cut(s) 156, 166
LguI GCTCTTC 1 cut(s) 203
LmnI GCTCC 1 cut(s) 193
LpnPI CCDG 4 cut(s) 12, 141, 173, 234
LweI GCATC 2 cut(s) 58, 88
MaeII ACGT 1 cut(s) 211
MalI GATC 2 cut(s) 158, 168
MboI GATC 2 cut(s) 156, 166
MboII GAAGA 2 cut(s) 114, 190
MhlI GDGCHC 1 cut(s) 198
MluCI AATT 1 cut(s) 273
MmeI TCCRAC 1 cut(s) 232
MnlI CCTC 4 cut(s) 124, 184, 298, 315
MseI TTAA 4 cut(s) 45, 105, 227, 276
MspI CCGG 1 cut(s) 128
MvnI CGCG 1 cut(s) 95
MwoI GCNNNNNNNGC 2 cut(s) 11, 107
NdeII GATC 2 cut(s) 156, 166
NlaIII CATG 1 cut(s) 173
NmeAIII GCCGAG 1 cut(s) 93
PagI TCATGA 1 cut(s) 169
PciSI GCTCTTC 1 cut(s) 203
PfeI GAWTC 1 cut(s) 177
Psp124BI GAGCTC 1 cut(s) 198
SacI GAGCTC 1 cut(s) 198
SapI GCTCTTC 1 cut(s) 203
SaqAI TTAA 4 cut(s) 45, 105, 227, 276
Sau3AI GATC 2 cut(s) 156, 166
SduI GDGCHC 1 cut(s) 198
SetI ASST 4 cut(s) 198, 214, 318, 326
SfaNI GCATC 2 cut(s) 58, 88
Sse9I AATT 1 cut(s) 273
SsiI CCGC 1 cut(s) 95
SstI GAGCTC 1 cut(s) 198
StyI CCWWGG 1 cut(s) 261
TaiI ACGT 1 cut(s) 214
TaqI TCGA 2 cut(s) 159, 320
TaqII GACCGA 1 cut(s) 101
TasI AATT 1 cut(s) 273
TfiI GAWTC 1 cut(s) 177
Tru1I TTAA 4 cut(s) 45, 105, 227, 276
Tru9I TTAA 4 cut(s) 45, 105, 227, 276
TscAI CASTG 2 cut(s) 87, 192
TspDTI ATGAA 3 cut(s) 169, 245, 319
TspRI CASTG 2 cut(s) 87, 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.