FvH4_2g00130

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010368
Physical Location & Seq
Forward (+)
227585 .. 228764
1180 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g00130.t1

Sequence Viewer

Length: 513 bp
ATGAAGGCCCAACCCAACCCATTTTCCTCCTCCAAAGCCGATCGAGGACGTCAAAGGGTTGGCTGTCACTCAAAAGTTGAAGAGATGGCGAGTAGGAGGGATGGAGAGAACAAGGGTTTGCTGTGGAAGCTTCCAGTCCTCAAAACCCAGCAACTCGGCAAGTTGGGTCCCGCCTTTGGCTTCGGCGTTGGCTGTGGTGTCGGAATCGGCATCGGCCTCCTCGGCGGCGTAGGAATTGGTCCTGGGATTCCCGGCATGCAAGTTGCTTTTGGCGTTGGTGCTGGATGCGGAGTTGGGTTAGGATTTGGCTACGGCGCTGGCAAGGGCGTCGCTCGTGATGACATCCGAAAGTACTCCAATGTTGGCAATCTCTTTTATGGTGAAAATGTTGGCAGTCGTTCTGGAAATCCCACCTCCCAGGATGAAATAGGCGCTCTTGTTGACGAGCTTGTTGATAATACCAAGAAACTGGTCAGAGCTACTACAAGAGAGATCGACAAGTGGACAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

171

Amino Acids

17.69

Weight (kDa)

9.6

Isoelectric Point (pI)

20.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 52
AciI CCGC 3 cut(s) 171, 225, 288
AcyI GRCGYC 2 cut(s) 49, 327
AfaI GTAC 1 cut(s) 353
AfiI CCNNNNNNNGG 1 cut(s) 176
AgsI TTSAA 1 cut(s) 80
AjnI CCWGG 2 cut(s) 241, 417
AluBI AGCT 3 cut(s) 130, 448, 479
AluI AGCT 3 cut(s) 130, 448, 479
AoxI GGCC 2 cut(s) 6, 214
AspLEI GCGC 2 cut(s) 317, 434
AspS9I GGNCC 3 cut(s) 7, 167, 239
AsuC2I CCSGG 1 cut(s) 252
AsuHPI GGTGA 1 cut(s) 392
AvaII GGWCC 2 cut(s) 167, 239
BauI CACGAG 1 cut(s) 333
BccI CCATC 2 cut(s) 79, 95
BceAI ACGGC 1 cut(s) 328
BcgI CGANNNNNNTGC 2 cut(s) 310, 344
BciT130I CCWGG 2 cut(s) 243, 419
BcnI CCSGG 1 cut(s) 252
BfoI RGCGCY 2 cut(s) 318, 435
BglI GCCNNNNNGGC 1 cut(s) 222
BisI GCNGC 1 cut(s) 226
BlsI GCNGC 1 cut(s) 227
BmcAI AGTACT 1 cut(s) 353
Bme1390I CCNGG 3 cut(s) 243, 252, 419
Bme18I GGWCC 2 cut(s) 167, 239
BmgT120I GGNCC 3 cut(s) 7, 167, 239
BmiI GGNNCC 2 cut(s) 168, 169
BmrFI CCNGG 3 cut(s) 243, 252, 419
BmsI GCATC 2 cut(s) 219, 275
BpuMI CCSGG 1 cut(s) 252
BsaHI GRCGYC 2 cut(s) 49, 327
BsaJI CCNNGG 3 cut(s) 220, 242, 417
Bsc4I CCNNNNNNNGG 1 cut(s) 176
Bse1I ACTGG 2 cut(s) 134, 474
BseBI CCWGG 2 cut(s) 243, 419
BseDI CCNNGG 3 cut(s) 220, 242, 417
BseGI GGATG 4 cut(s) 106, 290, 342, 427
BseLI CCNNNNNNNGG 1 cut(s) 176
BseNI ACTGG 2 cut(s) 134, 474
BseRI GAGGAG 2 cut(s) 19, 209
BseYI CCCAGC 1 cut(s) 147
Bsh1285I CGRYCG 1 cut(s) 43
BshFI GGCC 2 cut(s) 8, 216
BsiEI CGRYCG 1 cut(s) 43
BsiSI CCGG 1 cut(s) 252
BslFI GGGAC 1 cut(s) 153
BslI CCNNNNNNNGG 1 cut(s) 176
BsmFI GGGAC 1 cut(s) 153
BsnI GGCC 2 cut(s) 8, 216
Bsp143I GATC 2 cut(s) 40, 492
BspACI CCGC 3 cut(s) 171, 225, 288
BspANI GGCC 2 cut(s) 8, 216
BspLI GGNNCC 2 cut(s) 168, 169
BsrI ACTGG 2 cut(s) 134, 474
BssECI CCNNGG 3 cut(s) 220, 242, 417
BssMI GATC 2 cut(s) 40, 492
BssNI GRCGYC 2 cut(s) 49, 327
BssSI CACGAG 1 cut(s) 333
Bst2BI CACGAG 1 cut(s) 333
Bst2UI CCWGG 2 cut(s) 243, 419
Bst6I CTCTTC 1 cut(s) 75
BstACI GRCGYC 2 cut(s) 49, 327
BstC8I GCNNGC 2 cut(s) 257, 319
BstF5I GGATG 4 cut(s) 106, 290, 342, 427
BstH2I RGCGCY 2 cut(s) 318, 435
BstHHI GCGC 2 cut(s) 317, 434
BstKTI GATC 2 cut(s) 43, 495
BstMBI GATC 2 cut(s) 40, 492
BstMCI CGRYCG 1 cut(s) 43
BstMWI GCNNNNNNNGC 2 cut(s) 127, 222
BstNI CCWGG 2 cut(s) 243, 419
BstNSI RCATGY 1 cut(s) 259
BstSCI CCNGG 3 cut(s) 241, 250, 417
BstXI CCANNNNNNTGG 1 cut(s) 469
BsuRI GGCC 2 cut(s) 8, 216
BtsCI GGATG 4 cut(s) 106, 290, 342, 427
Cac8I GCNNGC 2 cut(s) 257, 319
CfoI GCGC 2 cut(s) 317, 434
Cfr13I GGNCC 3 cut(s) 7, 167, 239
CseI GACGC 1 cut(s) 316
Csp6I GTAC 1 cut(s) 352
CviAII CATG 1 cut(s) 256
CviQI GTAC 1 cut(s) 352
DpnI GATC 2 cut(s) 42, 494
DpnII GATC 2 cut(s) 40, 492
Eam1104I CTCTTC 1 cut(s) 75
EarI CTCTTC 1 cut(s) 75
Eco47I GGWCC 2 cut(s) 167, 239
EcoO109I RGGNCCY 1 cut(s) 167
EcoRII CCWGG 2 cut(s) 241, 417
FaeI CATG 1 cut(s) 259
FaiI YATR 2 cut(s) 257, 378
FaqI GGGAC 1 cut(s) 153
FatI CATG 1 cut(s) 255
FauI CCCGC 1 cut(s) 178
Fnu4HI GCNGC 1 cut(s) 226
FokI GGATG 4 cut(s) 113, 297, 329, 434
Fsp4HI GCNGC 1 cut(s) 226
GlaI GCGC 2 cut(s) 316, 433
GluI GCNGC 1 cut(s) 226
GsaI CCCAGC 1 cut(s) 151
HaeII RGCGCY 2 cut(s) 318, 435
HaeIII GGCC 2 cut(s) 8, 216
HapII CCGG 1 cut(s) 252
HgaI GACGC 1 cut(s) 316
HhaI GCGC 2 cut(s) 317, 434
Hin1I GRCGYC 2 cut(s) 49, 327
Hin1II CATG 1 cut(s) 259
Hin6I GCGC 2 cut(s) 315, 432
HinP1I GCGC 2 cut(s) 315, 432
HincII GTYRAC 1 cut(s) 442
HindII GTYRAC 1 cut(s) 442
HindIII AAGCTT 1 cut(s) 128
HinfI GANTC 2 cut(s) 204, 247
HpaII CCGG 1 cut(s) 252
HphI GGTGA 1 cut(s) 392
Hpy166II GTNNAC 2 cut(s) 442, 504
Hpy188I TCNGA 3 cut(s) 203, 347, 476
Hpy188III TCNNGA 2 cut(s) 335, 402
Hpy8I GTNNAC 2 cut(s) 442, 504
Hpy99I CGWCG 1 cut(s) 332
HpyCH4IV ACGT 1 cut(s) 49
HpyCH4V TGCA 1 cut(s) 259
HpyF10VI GCNNNNNNNGC 2 cut(s) 127, 222
HpySE526I ACGT 1 cut(s) 49
Hsp92I GRCGYC 2 cut(s) 49, 327
Hsp92II CATG 1 cut(s) 259
HspAI GCGC 2 cut(s) 315, 432
KflI GGGWCCC 1 cut(s) 167
Kzo9I GATC 2 cut(s) 40, 492
LweI GCATC 2 cut(s) 219, 275
MaeII ACGT 1 cut(s) 49
MaeIII GTNAC 1 cut(s) 65
MalI GATC 2 cut(s) 42, 494
MboI GATC 2 cut(s) 40, 492
MboII GAAGA 1 cut(s) 92
MluCI AATT 1 cut(s) 234
MmeI TCCRAC 1 cut(s) 181
MnlI CCTC 8 cut(s) 37, 38, 40, 90, 149, 227, 230, 424
MspI CCGG 1 cut(s) 252
MspR9I CCNGG 3 cut(s) 243, 252, 419
MvaI CCWGG 2 cut(s) 243, 419
MwoI GCNNNNNNNGC 2 cut(s) 127, 222
NciI CCSGG 1 cut(s) 252
NdeII GATC 2 cut(s) 40, 492
NlaIII CATG 1 cut(s) 259
NlaIV GGNNCC 2 cut(s) 168, 169
NmeAIII GCCGAG 2 cut(s) 135, 201
NmuCI GTSAC 1 cut(s) 65
NspI RCATGY 1 cut(s) 259
PaeI GCATGC 1 cut(s) 259
PfeI GAWTC 2 cut(s) 204, 247
PkrI GCNGC 1 cut(s) 227
Ple19I CGATCG 1 cut(s) 43
PpuMI RGGWCCY 1 cut(s) 167
Psp5II RGGWCCY 1 cut(s) 167
Psp6I CCWGG 2 cut(s) 241, 417
PspFI CCCAGC 1 cut(s) 147
PspGI CCWGG 2 cut(s) 241, 417
PspN4I GGNNCC 2 cut(s) 168, 169
PspPI GGNCC 3 cut(s) 7, 167, 239
PspPPI RGGWCCY 1 cut(s) 167
PvuI CGATCG 1 cut(s) 43
RsaI GTAC 1 cut(s) 353
RsaNI GTAC 1 cut(s) 352
SatI GCNGC 1 cut(s) 226
Sau3AI GATC 2 cut(s) 40, 492
Sau96I GGNCC 3 cut(s) 7, 167, 239
ScaI AGTACT 1 cut(s) 353
ScrFI CCNGG 3 cut(s) 243, 252, 419
SetI ASST 5 cut(s) 52, 132, 416, 450, 481
SfaNI GCATC 2 cut(s) 219, 275
SinI GGWCC 2 cut(s) 167, 239
SphI GCATGC 1 cut(s) 259
Sse9I AATT 1 cut(s) 234
SsiI CCGC 3 cut(s) 171, 225, 288
StyD4I CCNGG 3 cut(s) 241, 250, 417
TaiI ACGT 1 cut(s) 52
TaqI TCGA 2 cut(s) 43, 495
TasI AATT 1 cut(s) 234
TatI WGTACW 1 cut(s) 351
TauI GCSGC 1 cut(s) 228
TfiI GAWTC 2 cut(s) 204, 247
TseFI GTSAC 1 cut(s) 65
Tsp45I GTSAC 1 cut(s) 65
TspDTI ATGAA 2 cut(s) 17, 438
VpaK11BI GGWCC 2 cut(s) 167, 239
XceI RCATGY 1 cut(s) 259
ZraI GACGTC 1 cut(s) 50
ZrmI AGTACT 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.