MD05G1000400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010368
Physical Location & Seq
Forward (+)
91535 .. 92221
687 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1000400.v1.1.491

Sequence Viewer

Length: 357 bp
ATGATGGGCAGCAGGGGGTGCAGAGAAGATGATGGAGGAGAGGGGAAGGGTTTGTTGTGGAAGCTTCCAATCCTTAGAACCCAGCAACTAGGGAAGGTGGGTCCCGCCTTTGGCCTCGGCGCTGGTTGCGGGTTGGGTTTTGGCATCGGCCTCCTCGGCGGATTTGGTCCTGGGATTCTTGGCTTGCAAGTTGGCTTTGGCCTTGGTGCTGGATGCGGGGTCGCCTTAGGATTCGGCTACGGTGTCGGAAAGGGCATTGCCTACGATGACAATCGCAGATACTCTAATGTAGGCAACCTTTTTCGTGGTGAAAATGTCGACATTTCTCCTGCTTCTCAAGATCTGCCTACTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

119

Amino Acids

11.92

Weight (kDa)

7.69

Isoelectric Point (pI)

26.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 318
AciI CCGC 4 cut(s) 105, 129, 159, 216
AfiI CCNNNNNNNGG 1 cut(s) 110
AjnI CCWGG 1 cut(s) 169
AluBI AGCT 1 cut(s) 64
AluI AGCT 1 cut(s) 64
AoxI GGCC 3 cut(s) 112, 148, 199
ApeKI GCWGC 1 cut(s) 9
AspLEI GCGC 1 cut(s) 122
AspS9I GGNCC 2 cut(s) 101, 167
AsuHPI GGTGA 1 cut(s) 320
AvaII GGWCC 2 cut(s) 101, 167
AxyI CCTNAGG 1 cut(s) 226
BbvI GCAGC 1 cut(s) 21
BccI CCATC 1 cut(s) 26
BciT130I CCWGG 1 cut(s) 171
BfaI CTAG 1 cut(s) 89
BfoI RGCGCY 1 cut(s) 123
BglI GCCNNNNNGGC 1 cut(s) 156
BglII AGATCT 1 cut(s) 340
BisI GCNGC 1 cut(s) 10
BlsI GCNGC 1 cut(s) 11
Bme1390I CCNGG 1 cut(s) 171
Bme18I GGWCC 2 cut(s) 101, 167
BmgT120I GGNCC 2 cut(s) 101, 167
BmiI GGNNCC 2 cut(s) 102, 103
BmrFI CCNGG 1 cut(s) 171
BmsI GCATC 2 cut(s) 153, 203
BpuEI CTTGAG 1 cut(s) 321
BsaBI GATNNNNATC 1 cut(s) 270
BsaJI CCNNGG 4 cut(s) 115, 154, 170, 202
Bsc4I CCNNNNNNNGG 1 cut(s) 110
Bse21I CCTNAGG 1 cut(s) 226
Bse3DI GCAATG 1 cut(s) 255
Bse8I GATNNNNATC 1 cut(s) 270
BseBI CCWGG 1 cut(s) 171
BseDI CCNNGG 4 cut(s) 115, 154, 170, 202
BseGI GGATG 1 cut(s) 218
BseJI GATNNNNATC 1 cut(s) 270
BseLI CCNNNNNNNGG 1 cut(s) 110
BseMI GCAATG 1 cut(s) 255
BseRI GAGGAG 2 cut(s) 51, 143
BseXI GCAGC 1 cut(s) 21
BseYI CCCAGC 1 cut(s) 81
BsgI GTGCAG 1 cut(s) 40
BshFI GGCC 3 cut(s) 114, 150, 201
BslFI GGGAC 1 cut(s) 87
BslI CCNNNNNNNGG 1 cut(s) 110
BsmFI GGGAC 1 cut(s) 87
BsnI GGCC 3 cut(s) 114, 150, 201
Bsp143I GATC 1 cut(s) 340
BspACI CCGC 4 cut(s) 105, 129, 159, 216
BspANI GGCC 3 cut(s) 114, 150, 201
BspLI GGNNCC 2 cut(s) 102, 103
BsrDI GCAATG 1 cut(s) 255
BssECI CCNNGG 4 cut(s) 115, 154, 170, 202
BssMI GATC 1 cut(s) 340
BssT1I CCWWGG 1 cut(s) 202
Bst2UI CCWGG 1 cut(s) 171
Bst4CI ACNGT 1 cut(s) 242
BstAPI GCANNNNNTGC 1 cut(s) 18
BstC8I GCNNGC 1 cut(s) 185
BstDEI CTNAG 3 cut(s) 74, 226, 351
BstF5I GGATG 1 cut(s) 218
BstH2I RGCGCY 1 cut(s) 123
BstHHI GCGC 1 cut(s) 122
BstKTI GATC 1 cut(s) 343
BstMBI GATC 1 cut(s) 340
BstMWI GCNNNNNNNGC 3 cut(s) 18, 126, 156
BstNI CCWGG 1 cut(s) 171
BstSCI CCNGG 1 cut(s) 169
BstV1I GCAGC 1 cut(s) 21
BstX2I RGATCY 1 cut(s) 340
BstYI RGATCY 1 cut(s) 340
Bsu36I CCTNAGG 1 cut(s) 226
BsuRI GGCC 3 cut(s) 114, 150, 201
BtsCI GGATG 1 cut(s) 218
Cac8I GCNNGC 1 cut(s) 185
CfoI GCGC 1 cut(s) 122
Cfr13I GGNCC 2 cut(s) 101, 167
CviJI RGCY 7 cut(s) 64, 114, 150, 183, 195, 201, 237
CviKI_1 RGCY 7 cut(s) 64, 114, 150, 183, 195, 201, 237
DdeI CTNAG 3 cut(s) 74, 226, 351
DpnI GATC 1 cut(s) 342
DpnII GATC 1 cut(s) 340
EciI GGCGGA 1 cut(s) 174
Eco130I CCWWGG 1 cut(s) 202
Eco47I GGWCC 2 cut(s) 101, 167
Eco81I CCTNAGG 1 cut(s) 226
EcoO109I RGGNCCY 1 cut(s) 101
EcoRII CCWGG 1 cut(s) 169
EcoT14I CCWWGG 1 cut(s) 202
ErhI CCWWGG 1 cut(s) 202
FaqI GGGAC 1 cut(s) 87
FauI CCCGC 3 cut(s) 112, 122, 209
FblI GTMKAC 1 cut(s) 318
Fnu4HI GCNGC 1 cut(s) 10
FokI GGATG 1 cut(s) 225
Fsp4HI GCNGC 1 cut(s) 10
FspBI CTAG 1 cut(s) 89
GlaI GCGC 1 cut(s) 121
GluI GCNGC 1 cut(s) 10
GsaI CCCAGC 1 cut(s) 85
HaeII RGCGCY 1 cut(s) 123
HaeIII GGCC 3 cut(s) 114, 150, 201
HhaI GCGC 1 cut(s) 122
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HincII GTYRAC 1 cut(s) 319
HindII GTYRAC 1 cut(s) 319
HindIII AAGCTT 1 cut(s) 62
HinfI GANTC 2 cut(s) 175, 231
HphI GGTGA 1 cut(s) 320
Hpy166II GTNNAC 1 cut(s) 319
Hpy188I TCNGA 1 cut(s) 248
Hpy188III TCNNGA 1 cut(s) 338
Hpy8I GTNNAC 1 cut(s) 319
HpyAV CCTTC 2 cut(s) 40, 88
HpyCH4III ACNGT 1 cut(s) 242
HpyCH4V TGCA 2 cut(s) 21, 187
HpyF10VI GCNNNNNNNGC 3 cut(s) 18, 126, 156
HpyF3I CTNAG 3 cut(s) 74, 226, 351
HspAI GCGC 1 cut(s) 120
KflI GGGWCCC 1 cut(s) 101
Kzo9I GATC 1 cut(s) 340
LpnPI CCDG 6 cut(s) 95, 108, 156, 183, 195, 342
Lsp1109I GCAGC 1 cut(s) 21
LweI GCATC 2 cut(s) 153, 203
MaeI CTAG 1 cut(s) 89
MalI GATC 1 cut(s) 342
MboI GATC 1 cut(s) 340
MboII GAAGA 1 cut(s) 38
MflI RGATCY 1 cut(s) 340
MmeI TCCRAC 1 cut(s) 226
MnlI CCTC 5 cut(s) 29, 34, 125, 161, 164
MspR9I CCNGG 1 cut(s) 171
MvaI CCWGG 1 cut(s) 171
MwoI GCNNNNNNNGC 3 cut(s) 18, 126, 156
NdeII GATC 1 cut(s) 340
NlaIV GGNNCC 2 cut(s) 102, 103
NmeAIII GCCGAG 2 cut(s) 96, 135
PfeI GAWTC 2 cut(s) 175, 231
PkrI GCNGC 1 cut(s) 11
PpuMI RGGWCCY 1 cut(s) 101
Psp5II RGGWCCY 1 cut(s) 101
Psp6I CCWGG 1 cut(s) 169
PspFI CCCAGC 1 cut(s) 81
PspGI CCWGG 1 cut(s) 169
PspN4I GGNNCC 2 cut(s) 102, 103
PspPI GGNCC 2 cut(s) 101, 167
PspPPI RGGWCCY 1 cut(s) 101
PsuI RGATCY 1 cut(s) 340
SalI GTCGAC 1 cut(s) 317
SatI GCNGC 1 cut(s) 10
Sau3AI GATC 1 cut(s) 340
Sau96I GGNCC 2 cut(s) 101, 167
ScrFI CCNGG 1 cut(s) 171
SetI ASST 3 cut(s) 66, 99, 300
SfaNI GCATC 2 cut(s) 153, 203
SinI GGWCC 2 cut(s) 101, 167
SmlI CTYRAG 1 cut(s) 336
SmoI CTYRAG 1 cut(s) 336
SsiI CCGC 4 cut(s) 105, 129, 159, 216
SspMI CTAG 1 cut(s) 89
StyD4I CCNGG 1 cut(s) 169
StyI CCWWGG 1 cut(s) 202
TaaI ACNGT 1 cut(s) 242
TaqI TCGA 1 cut(s) 318
TfiI GAWTC 2 cut(s) 175, 231
TseI GCWGC 1 cut(s) 9
VpaK11BI GGWCC 2 cut(s) 101, 167
XmiI GTMKAC 1 cut(s) 318
XspI CTAG 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.