FvH4_2g01561

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0016251
Physical Location & Seq
Forward (+)
1416252 .. 1417917
1666 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g01561.t1

Sequence Viewer

Length: 1011 bp
ATGGGGACTGAGTCTCTTTTCCAGAGAACTGAAGTAGAGTCAACAGAAGAGGGACAACCTCCTTCTCAATACAATATACAAGAATTAGACAGAAACCGATTTGCTTCTTGTAATTCTTATAATGGCAAACGGCGACAGTGCAGCAACCATTGGCAGGCACCACCTGACGATGTGATGGAGAAGATTTTACAGCGCCTTAGTTTGAAGGATCGAATACCTGCAAGTCATGGCAGTCGATTGTTATGCGAAGAGACATCCGTAGTGCTCCGCATGAACCTCCATGGATATTCAACTTTGGCTTTAAACAGACCTGGAGACGAGAGGTGGACTATTCTCTTAGAGCTACCAGGTCGTGAAACCAAAAACTTTTTTGATGATATATTGTTTTCTTCTGGTGCGTTACATGCCTGCGTTCGCTATACTTCTAAAGATAAAGCAATATTGATGAGCGTTGAAGATCGAACTCTAAACTTTGGAGATCAAGTAATGGAATTAAAGTTGGTTCACTTCGTTGATGATAGACGTGGTAGATATATCCCACGTCTGTTAGAATCAAACAATGAGGTACTGTTCGTGATACAAATTTTAGATGAGCTCGATGATGGTGATGTTGAGGATTATGGTTATCATGGTGAAGGTAATGACCATGATGATATTGATGGTGATGTCGATGAATATTATCTTGCTGATGAAGCCACTGTAGTTGCTGAACATGATAATGAAAACGTCAACGACTTAGAGGACCATGGTTATTTGTCCACCTCCGCCATGGATAACAAGGAGCTAGAGACCAACCGCATTTTTTTCGCAACGAAAAGCTGTGTTCGCTGGAAGCCTCCTTGTGTAGAACCGCCAACCGTTGCGTCTCATGATATCGGCGTATTCAACCTAGAGAGTGGAAGAACTGAGCAAAACTTTCCAAGTATCAACATGTCAGTGTGGTCTCGACTTGATGGTCTACTGGAAAAGGATATAATGAGATTCAGTTGGTTTACCCCAAGAAAGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

337

Amino Acids

38.72

Weight (kDa)

4.74

Isoelectric Point (pI)

37.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 86 - 296 5.4e-06 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 120
AasI GACNNNNNNGTC 1 cut(s) 954
Acc36I ACCTGC 1 cut(s) 226
AccB1I GGYRCC 1 cut(s) 157
AccI GTMKAC 1 cut(s) 958
AciI CCGC 4 cut(s) 268, 765, 796, 851
AclWI GGATC 1 cut(s) 216
AcsI RAATTY 1 cut(s) 582
AcuI CTGAAG 1 cut(s) 51
AfaI GTAC 1 cut(s) 567
AfiI CCNNNNNNNGG 1 cut(s) 154
AflIII ACRYGT 1 cut(s) 930
AgsI TTSAA 4 cut(s) 205, 291, 455, 886
AjiI CACGTC 2 cut(s) 524, 542
AjnI CCWGG 2 cut(s) 310, 346
AluBI AGCT 4 cut(s) 343, 595, 784, 819
AluI AGCT 4 cut(s) 343, 595, 784, 819
Alw21I GWGCWC 2 cut(s) 267, 597
Alw26I GTCTC 6 cut(s) 18, 245, 309, 782, 870, 948
AlwI GGATC 1 cut(s) 216
ApeKI GCWGC 1 cut(s) 141
ApoI RAATTY 1 cut(s) 582
AspLEI GCGC 1 cut(s) 195
AspS9I GGNCC 1 cut(s) 742
AsuHPI GGTGA 3 cut(s) 617, 644, 674
AvaII GGWCC 1 cut(s) 742
BanI GGYRCC 1 cut(s) 157
BanII GRGCYC 1 cut(s) 597
Bbv12I GWGCWC 2 cut(s) 267, 597
BbvI GCAGC 1 cut(s) 153
BccI CCATC 4 cut(s) 169, 596, 653, 947
BceAI ACGGC 1 cut(s) 146
BcgI CGANNNNNNTGC 4 cut(s) 225, 259, 787, 821
BciT130I CCWGG 2 cut(s) 312, 348
BcoDI GTCTC 6 cut(s) 18, 245, 309, 782, 870, 948
BfaI CTAG 2 cut(s) 785, 890
BfmI CTRYAG 1 cut(s) 699
BfoI RGCGCY 1 cut(s) 196
BfuAI ACCTGC 1 cut(s) 226
BisI GCNGC 1 cut(s) 142
BlsI GCNGC 1 cut(s) 143
Bme1390I CCNGG 2 cut(s) 312, 348
Bme18I GGWCC 1 cut(s) 742
BmgBI CACGTC 2 cut(s) 524, 542
BmgT120I GGNCC 1 cut(s) 742
BmiI GGNNCC 1 cut(s) 159
BmrFI CCNGG 2 cut(s) 312, 348
BpmI CTGGAG 1 cut(s) 333
BsaI GGTCTC 2 cut(s) 782, 948
BsaJI CCNNGG 3 cut(s) 280, 745, 768
BsaXI ACNNNNNCTCC 2 cut(s) 468, 498
Bsc4I CCNNNNNNNGG 1 cut(s) 154
Bse1I ACTGG 1 cut(s) 966
BseBI CCWGG 2 cut(s) 312, 348
BseDI CCNNGG 3 cut(s) 280, 745, 768
BseGI GGATG 1 cut(s) 254
BseLI CCNNNNNNNGG 1 cut(s) 154
BseMII CTCAG 1 cut(s) 897
BseNI ACTGG 1 cut(s) 966
BseXI GCAGC 1 cut(s) 153
BsgI GTGCAG 1 cut(s) 160
BshNI GGYRCC 1 cut(s) 157
BsiHKAI GWGCWC 2 cut(s) 267, 597
BslFI GGGAC 2 cut(s) 19, 66
BslI CCNNNNNNNGG 1 cut(s) 154
BsmAI GTCTC 6 cut(s) 18, 245, 309, 782, 870, 948
BsmBI CGTCTC 2 cut(s) 309, 870
BsmFI GGGAC 2 cut(s) 19, 66
Bso31I GGTCTC 2 cut(s) 782, 948
Bsp1286I GDGCHC 2 cut(s) 267, 597
Bsp143I GATC 3 cut(s) 208, 457, 478
Bsp19I CCATGG 3 cut(s) 280, 745, 768
BspACI CCGC 4 cut(s) 268, 765, 796, 851
BspCNI CTCAG 1 cut(s) 898
BspHI TCATGA 1 cut(s) 868
BspLI GGNNCC 1 cut(s) 159
BspMI ACCTGC 1 cut(s) 226
BspPI GGATC 1 cut(s) 216
BspT107I GGYRCC 1 cut(s) 157
BspTNI GGTCTC 2 cut(s) 782, 948
BsrI ACTGG 1 cut(s) 966
BssECI CCNNGG 3 cut(s) 280, 745, 768
BssMI GATC 3 cut(s) 208, 457, 478
BssT1I CCWWGG 3 cut(s) 280, 745, 768
Bst2UI CCWGG 2 cut(s) 312, 348
Bst4CI ACNGT 4 cut(s) 138, 570, 700, 859
Bst6I CTCTTC 2 cut(s) 42, 243
BstC8I GCNNGC 2 cut(s) 156, 409
BstDEI CTNAG 5 cut(s) 9, 197, 337, 736, 906
BstDSI CCRYGG 3 cut(s) 280, 745, 768
BstF5I GGATG 1 cut(s) 254
BstH2I RGCGCY 1 cut(s) 196
BstHHI GCGC 1 cut(s) 195
BstKTI GATC 3 cut(s) 211, 460, 481
BstMAI GTCTC 6 cut(s) 18, 245, 309, 782, 870, 948
BstMBI GATC 3 cut(s) 208, 457, 478
BstMWI GCNNNNNNNGC 3 cut(s) 404, 692, 825
BstNI CCWGG 2 cut(s) 312, 348
BstNSI RCATGY 2 cut(s) 407, 934
BstSCI CCNGG 2 cut(s) 310, 346
BstSFI CTRYAG 1 cut(s) 699
BstV1I GCAGC 1 cut(s) 153
BstXI CCANNNNNNTGG 1 cut(s) 1005
BtgI CCRYGG 3 cut(s) 280, 745, 768
BtrI CACGTC 2 cut(s) 524, 542
BtsCI GGATG 1 cut(s) 254
BtsIMutI CAGTG 3 cut(s) 143, 696, 942
BveI ACCTGC 1 cut(s) 226
Cac8I GCNNGC 2 cut(s) 156, 409
CciI TCATGA 1 cut(s) 868
CfoI GCGC 1 cut(s) 195
Cfr13I GGNCC 1 cut(s) 742
CseI GACGC 1 cut(s) 852
CsiI ACCWGGT 1 cut(s) 346
Csp6I GTAC 1 cut(s) 566
CspCI CAANNNNNGTGG 2 cut(s) 685, 720
CviJI RGCY 7 cut(s) 299, 343, 595, 695, 784, 819, 835
CviKI_1 RGCY 7 cut(s) 299, 343, 595, 695, 784, 819, 835
CviQI GTAC 1 cut(s) 566
DdeI CTNAG 5 cut(s) 9, 197, 337, 736, 906
DpnI GATC 3 cut(s) 210, 459, 480
DpnII GATC 3 cut(s) 208, 457, 478
DraI TTTAAA 1 cut(s) 303
DrdI GACNNNNNNGTC 1 cut(s) 954
DseDI GACNNNNNNGTC 1 cut(s) 954
Eam1104I CTCTTC 2 cut(s) 42, 243
EarI CTCTTC 2 cut(s) 42, 243
EciI GGCGGA 1 cut(s) 754
Ecl136II GAGCTC 1 cut(s) 595
Eco130I CCWWGG 3 cut(s) 280, 745, 768
Eco24I GRGCYC 1 cut(s) 597
Eco31I GGTCTC 2 cut(s) 782, 948
Eco32I GATATC 1 cut(s) 874
Eco47I GGWCC 1 cut(s) 742
Eco53kI GAGCTC 1 cut(s) 595
Eco57I CTGAAG 1 cut(s) 51
EcoICRI GAGCTC 1 cut(s) 595
EcoRII CCWGG 2 cut(s) 310, 346
EcoRV GATATC 1 cut(s) 874
EcoT14I CCWWGG 3 cut(s) 280, 745, 768
EcoT38I GRGCYC 1 cut(s) 597
ErhI CCWWGG 3 cut(s) 280, 745, 768
Esp3I CGTCTC 2 cut(s) 309, 870
FaqI GGGAC 2 cut(s) 19, 66
FblI GTMKAC 1 cut(s) 958
Fnu4HI GCNGC 1 cut(s) 142
FokI GGATG 1 cut(s) 241
FriOI GRGCYC 1 cut(s) 597
Fsp4HI GCNGC 1 cut(s) 142
FspBI CTAG 2 cut(s) 785, 890
GlaI GCGC 1 cut(s) 194
GluI GCNGC 1 cut(s) 142
GsuI CTGGAG 1 cut(s) 333
HaeII RGCGCY 1 cut(s) 196
HgaI GACGC 1 cut(s) 852
HhaI GCGC 1 cut(s) 195
Hin6I GCGC 1 cut(s) 193
HinP1I GCGC 1 cut(s) 193
HincII GTYRAC 2 cut(s) 42, 730
HindII GTYRAC 2 cut(s) 42, 730
HinfI GANTC 4 cut(s) 11, 38, 551, 981
HphI GGTGA 3 cut(s) 617, 644, 674
Hpy166II GTNNAC 7 cut(s) 42, 327, 505, 730, 759, 959, 993
Hpy188III TCNNGA 5 cut(s) 22, 353, 574, 869, 945
Hpy8I GTNNAC 7 cut(s) 42, 327, 505, 730, 759, 959, 993
HpyAV CCTTC 3 cut(s) 72, 199, 629
HpyCH4III ACNGT 4 cut(s) 138, 570, 700, 859
HpyCH4IV ACGT 3 cut(s) 523, 541, 726
HpyCH4V TGCA 2 cut(s) 141, 221
HpyF10VI GCNNNNNNNGC 3 cut(s) 404, 692, 825
HpyF3I CTNAG 5 cut(s) 9, 197, 337, 736, 906
HpySE526I ACGT 3 cut(s) 523, 541, 726
HspAI GCGC 1 cut(s) 193
Kzo9I GATC 3 cut(s) 208, 457, 478
LmnI GCTCC 2 cut(s) 270, 781
Lsp1109I GCAGC 1 cut(s) 153
MabI ACCWGGT 1 cut(s) 346
MaeI CTAG 2 cut(s) 785, 890
MaeII ACGT 3 cut(s) 523, 541, 726
MaeIII GTNAC 1 cut(s) 399
MalI GATC 3 cut(s) 210, 459, 480
MboI GATC 3 cut(s) 208, 457, 478
MboII GAAGA 6 cut(s) 59, 193, 260, 381, 467, 912
MhlI GDGCHC 2 cut(s) 267, 597
MluCI AATT 4 cut(s) 83, 112, 491, 582
MlyI GAGTC 2 cut(s) 20, 47
MnlI CCTC 9 cut(s) 43, 69, 287, 315, 556, 607, 733, 772, 846
MseI TTAA 2 cut(s) 302, 494
MslI CAYNNNNRTG 2 cut(s) 717, 935
MspR9I CCNGG 2 cut(s) 312, 348
MvaI CCWGG 2 cut(s) 312, 348
MwoI GCNNNNNNNGC 3 cut(s) 404, 692, 825
NcoI CCATGG 3 cut(s) 280, 745, 768
NdeII GATC 3 cut(s) 208, 457, 478
NlaIV GGNNCC 1 cut(s) 159
NspI RCATGY 2 cut(s) 407, 934
PagI TCATGA 1 cut(s) 868
PciI ACATGT 1 cut(s) 930
PfeI GAWTC 2 cut(s) 551, 981
PflFI GACNNNGTC 1 cut(s) 10
PkrI GCNGC 1 cut(s) 143
PleI GAGTC 2 cut(s) 19, 46
PpsI GAGTC 2 cut(s) 19, 46
PscI ACATGT 1 cut(s) 930
PsiI TTATAA 1 cut(s) 120
Psp124BI GAGCTC 1 cut(s) 597
Psp6I CCWGG 2 cut(s) 310, 346
PspGI CCWGG 2 cut(s) 310, 346
PspN4I GGNNCC 1 cut(s) 159
PspPI GGNCC 1 cut(s) 742
PsyI GACNNNGTC 1 cut(s) 10
RsaI GTAC 1 cut(s) 567
RsaNI GTAC 1 cut(s) 566
RseI CAYNNNNRTG 2 cut(s) 717, 935
SacI GAGCTC 1 cut(s) 597
SaqAI TTAA 2 cut(s) 302, 494
SatI GCNGC 1 cut(s) 142
Sau3AI GATC 3 cut(s) 208, 457, 478
Sau96I GGNCC 1 cut(s) 742
SchI GAGTC 2 cut(s) 20, 47
ScrFI CCNGG 2 cut(s) 312, 348
SduI GDGCHC 2 cut(s) 267, 597
SexAI ACCWGGT 1 cut(s) 346
SfcI CTRYAG 1 cut(s) 699
SinI GGWCC 1 cut(s) 742
SmiMI CAYNNNNRTG 2 cut(s) 717, 935
Sse9I AATT 4 cut(s) 83, 112, 491, 582
SsiI CCGC 4 cut(s) 268, 765, 796, 851
SspI AATATT 2 cut(s) 441, 677
SspMI CTAG 2 cut(s) 785, 890
SstI GAGCTC 1 cut(s) 597
StyD4I CCNGG 2 cut(s) 310, 346
StyI CCWWGG 3 cut(s) 280, 745, 768
TaaI ACNGT 4 cut(s) 138, 570, 700, 859
TaiI ACGT 3 cut(s) 526, 544, 729
TaqI TCGA 6 cut(s) 211, 235, 460, 597, 669, 946
TasI AATT 4 cut(s) 83, 112, 491, 582
TfiI GAWTC 2 cut(s) 551, 981
Tru1I TTAA 2 cut(s) 302, 494
Tru9I TTAA 2 cut(s) 302, 494
TscAI CASTG 3 cut(s) 143, 703, 942
TseI GCWGC 1 cut(s) 141
TspDTI ATGAA 4 cut(s) 287, 687, 705, 735
TspGWI ACGGA 1 cut(s) 247
TspRI CASTG 3 cut(s) 143, 703, 942
Tth111I GACNNNGTC 1 cut(s) 10
VpaK11BI GGWCC 1 cut(s) 742
XapI RAATTY 1 cut(s) 582
XceI RCATGY 2 cut(s) 407, 934
XcmI CCANNNNNNNNNTGG 1 cut(s) 766
XmiI GTMKAC 1 cut(s) 958
XspI CTAG 2 cut(s) 785, 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.