FvH4_2g01970

Proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010868
Physical Location & Seq
Reverse (-)
1683146 .. 1685359
2214 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g01970.t1

Sequence Viewer

Length: 1350 bp
ATGAAGATCTCTCATCTGTGCCTCTTCTTGTCCTTGTTGATCTTTGGAGCCAGCAGCTTTTGCTACGCCGATGATCATAAGACAGTTGAGGTAGTTGGTTTTGGAGAATGTACCGACTGTGCTGAGAAGAGCATTAAGACCATTCATGCTTTTTCAGGTCTAGGTGTTACAATCGACTGCAAGACCGAAAGCGGCAACTTCGAGAGGAGAGGAGCCGGCAAGTTCAATGAGGAAGGCAAGTTTTCGGTGTCGATCCCGAAAGAGATTGTGAAAGATGGAGGATTGAAGGAGGAGTGCTATGCACAGCTCCACAATGCAAAGGCCACACCATGTGGTGCTCATGATGGACTTGATTCCACCAAAGTGGTGTTCATTTCTAAAAACAGTGATGGCAAACAAACCTTTGGAATTGCCGGTACTAAACTGAAATTCTCACCAGCTACATGCCCTTCTGCTTTCTTTTGGCCTCACTTTAAACACCCTCTTCCCAAATTGACCAAACCACACCCTTTCTTTAAACACCCTCTTCCCAAATTGACCAAACCACACCCTTTCTTTAAACACCCTCCAATTTTCCCTTTCCCTCCAAAAATTTACCCTCCATTCCCACCCAAGGTCACTATTCCTCCATTCTATAAGAAACCCTTCCCTCCCAAGGTCACTATTCCTCCATTCTATAAGAAACCCTGCCCTCCCAAGATCCCTATTTTCAAGAAGCCTCTCCCCCCACCAGTCCCAGTTTATGTAAAGCCCCTCCCTCCACCAGTCCCAGTTTACCAAAAGCCTCTCCCTCCACCAGTCCCAGTCTACAAGAAGCCACTCCCTCCACCAGTCCCAGTCTACGAGAAACCAAACCCTCCACCAGTTCCAGTCTACAAGAAACCATGCCCTCCACCAGTTCCAGTGAAGAAACCACAACCTCCACCAGTCCCAGTTTACAAAAAGCCCCATCCTCCAAAAGCACCCAAAAAAGTATGCCCTCCACCAGTCCCTGTCAAGAAGCCAACTCCACCACCAGTCCCTGTGTACAAAAAGCCACTTCCACCGCCGGTTCCGGTGTACACAAAACCGCTTCCACCGCCGGTTCCGGTGTACACAAAACCGCTTCCACCGCCGGTTCCCGTGTACACAAAACCGCTTCCACCACCTGTCCCAGTGTACACAAAACCACTGCCACCTCCAATCCCATTTTACAAGCCAAAACCGCACCCATGGTTCAAGCCAAAGCCACACCCATGGTTCAAGCACCCATTCTTGAAGCCTCTGCCTCCATTCCCCAAGATCCCTCATCATCCTTTCTTTAAGAAACCACCACCATACCCACCACAGAGTACTTCTCCCAAGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

450

Amino Acids

50.03

Weight (kDa)

9.91

Isoelectric Point (pI)

59.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 31 - 113 1.9e-09 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010868)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21140 AT4G38770
fragaria_vesca FvH4_2g01970
malus_domestica MD05G1116400.v1.1 MD10G1118600.v1.1
prunus_persica Prupe.8G160900_v2.0.a1
pyrus_communis pycom05g11060 pycom10g10270
rosa_chinensis RchiOBHm_Chr6g0245161
rosa_laevigata RLG00000015320
rosa_multiflora Rmu_sc0001288.1_g000002 Rmu_sc0015012.1_g000001 Rmu_ssc0000087.1_g000001
rosa_roxburghii Rroxscaffold_7G00215400
rosa_rugosa Rorug05G0512400
rosa_samantha Rh6AG027500 Rh6BG023600 Rh6CG022000 Rh6DG022100
rosa_wichuraiana Rw6G002320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 807, 840, 873
AciI CCGC 8 cut(s) 192, 1046, 1070, 1079, 1103, 1112, 1136, 1205
AclWI GGATC 3 cut(s) 247, 694, 1276
AcsI RAATTY 2 cut(s) 428, 591
AdeI CACNNNGTG 1 cut(s) 332
AfaI GTAC 8 cut(s) 112, 418, 1028, 1061, 1094, 1127, 1160, 1333
AfiI CCNNNNNNNGG 2 cut(s) 613, 655
AgsI TTSAA 6 cut(s) 226, 286, 712, 1219, 1243, 1258
AjuI GAANNNNNNNTTGG 2 cut(s) 353, 385
AleI CACNNNNGTG 1 cut(s) 362
AluBI AGCT 3 cut(s) 57, 307, 440
AluI AGCT 3 cut(s) 57, 307, 440
Alw21I GWGCWC 1 cut(s) 340
AlwI GGATC 3 cut(s) 247, 694, 1276
AlwNI CAGNNNCTG 2 cut(s) 992, 1022
AoxI GGCC 2 cut(s) 321, 464
ApeKI GCWGC 1 cut(s) 54
ApoI RAATTY 2 cut(s) 428, 591
Asp700I GAANNNNTTC 1 cut(s) 644
AsuHPI GGTGA 1 cut(s) 426
Bbv12I GWGCWC 1 cut(s) 340
BbvI GCAGC 1 cut(s) 66
BccI CCATC 4 cut(s) 269, 338, 383, 957
BclI TGATCA 1 cut(s) 73
BfaI CTAG 1 cut(s) 161
BglII AGATCT 1 cut(s) 6
BisI GCNGC 2 cut(s) 55, 193
BlsI GCNGC 2 cut(s) 56, 194
BmcAI AGTACT 1 cut(s) 1333
BmiI GGNNCC 5 cut(s) 49, 214, 1053, 1086, 1119
BmrI ACTGGG 6 cut(s) 731, 764, 797, 830, 926, 1148
BmuI ACTGGG 6 cut(s) 731, 764, 797, 830, 926, 1148
BplI GAGNNNNNCTC 1 cut(s) 1321
BsaJI CCNNGG 5 cut(s) 612, 654, 1211, 1235, 1341
BsaWI WCCGGW 2 cut(s) 1054, 1087
BsaXI ACNNNNNCTCC 4 cut(s) 610, 640, 652, 682
Bsc4I CCNNNNNNNGG 2 cut(s) 613, 655
Bse118I RCCGGY 5 cut(s) 215, 413, 1048, 1081, 1114
BseDI CCNNGG 5 cut(s) 612, 654, 1211, 1235, 1341
BseGI GGATG 2 cut(s) 949, 1291
BseLI CCNNNNNNNGG 2 cut(s) 613, 655
BseMII CTCAG 1 cut(s) 114
BseRI GAGGAG 3 cut(s) 220, 225, 305
BseXI GCAGC 1 cut(s) 66
BshFI GGCC 2 cut(s) 323, 466
BsiHKAI GWGCWC 1 cut(s) 340
BsiSI CCGG 7 cut(s) 216, 414, 1049, 1055, 1082, 1088, 1115
BslFI GGGAC 8 cut(s) 719, 752, 785, 818, 914, 974, 1004, 1136
BslI CCNNNNNNNGG 2 cut(s) 613, 655
BsmFI GGGAC 8 cut(s) 719, 752, 785, 818, 914, 974, 1004, 1136
BsnI GGCC 2 cut(s) 323, 466
Bsp1286I GDGCHC 1 cut(s) 340
Bsp1407I TGTACA 5 cut(s) 1026, 1059, 1092, 1125, 1158
Bsp143I GATC 6 cut(s) 6, 39, 73, 252, 699, 1281
Bsp19I CCATGG 2 cut(s) 1211, 1235
BspACI CCGC 8 cut(s) 192, 1046, 1070, 1079, 1103, 1112, 1136, 1205
BspANI GGCC 2 cut(s) 323, 466
BspCNI CTCAG 1 cut(s) 115
BspHI TCATGA 1 cut(s) 340
BspLI GGNNCC 5 cut(s) 49, 214, 1053, 1086, 1119
BspPI GGATC 3 cut(s) 247, 694, 1276
BspQI GCTCTTC 1 cut(s) 122
BsrFI RCCGGY 5 cut(s) 215, 413, 1048, 1081, 1114
BsrGI TGTACA 5 cut(s) 1026, 1059, 1092, 1125, 1158
BssAI RCCGGY 5 cut(s) 215, 413, 1048, 1081, 1114
BssECI CCNNGG 5 cut(s) 612, 654, 1211, 1235, 1341
BssMI GATC 6 cut(s) 6, 39, 73, 252, 699, 1281
BssT1I CCWWGG 5 cut(s) 612, 654, 1211, 1235, 1341
Bst4CI ACNGT 3 cut(s) 85, 119, 386
Bst6I CTCTTC 4 cut(s) 29, 122, 489, 531
BstAPI GCANNNNNTGC 1 cut(s) 60
BstAUI TGTACA 5 cut(s) 1026, 1059, 1092, 1125, 1158
BstC8I GCNNGC 2 cut(s) 52, 217
BstDEI CTNAG 1 cut(s) 123
BstDSI CCRYGG 2 cut(s) 1211, 1235
BstF5I GGATG 2 cut(s) 949, 1291
BstKTI GATC 6 cut(s) 9, 42, 76, 255, 702, 1284
BstMBI GATC 6 cut(s) 6, 39, 73, 252, 699, 1281
BstMWI GCNNNNNNNGC 4 cut(s) 60, 1078, 1111, 1204
BstNSI RCATGY 1 cut(s) 447
BstV1I GCAGC 1 cut(s) 66
BstX2I RGATCY 3 cut(s) 6, 699, 1281
BstXI CCANNNNNNTGG 2 cut(s) 364, 1236
BstYI RGATCY 3 cut(s) 6, 699, 1281
BsuRI GGCC 2 cut(s) 323, 466
BtgI CCRYGG 2 cut(s) 1211, 1235
BtsCI GGATG 2 cut(s) 949, 1291
BtsI GCAGTG 1 cut(s) 1169
BtsIMutI CAGTG 4 cut(s) 391, 909, 1161, 1169
Cac8I GCNNGC 2 cut(s) 52, 217
CaiI CAGNNNCTG 2 cut(s) 992, 1022
CciI TCATGA 1 cut(s) 340
Cfr10I RCCGGY 5 cut(s) 215, 413, 1048, 1081, 1114
Csp6I GTAC 8 cut(s) 111, 417, 1027, 1060, 1093, 1126, 1159, 1332
CviAII CATG 7 cut(s) 146, 330, 341, 444, 885, 1212, 1236
CviQI GTAC 8 cut(s) 111, 417, 1027, 1060, 1093, 1126, 1159, 1332
DdeI CTNAG 1 cut(s) 123
DpnI GATC 6 cut(s) 8, 41, 75, 254, 701, 1283
DpnII GATC 6 cut(s) 6, 39, 73, 252, 699, 1281
DraI TTTAAA 3 cut(s) 475, 517, 559
DraIII CACNNNGTG 1 cut(s) 332
Eam1104I CTCTTC 4 cut(s) 29, 122, 489, 531
EarI CTCTTC 4 cut(s) 29, 122, 489, 531
Eco130I CCWWGG 5 cut(s) 612, 654, 1211, 1235, 1341
EcoT14I CCWWGG 5 cut(s) 612, 654, 1211, 1235, 1341
ErhI CCWWGG 5 cut(s) 612, 654, 1211, 1235, 1341
FaeI CATG 7 cut(s) 149, 333, 344, 447, 888, 1215, 1239
FalI AAGNNNNNCTT 2 cut(s) 629, 661
FaqI GGGAC 8 cut(s) 719, 752, 785, 818, 914, 974, 1004, 1136
FatI CATG 7 cut(s) 145, 329, 340, 443, 884, 1211, 1235
FbaI TGATCA 1 cut(s) 73
FblI GTMKAC 3 cut(s) 807, 840, 873
Fnu4HI GCNGC 2 cut(s) 55, 193
FokI GGATG 2 cut(s) 936, 1278
Fsp4HI GCNGC 2 cut(s) 55, 193
FspBI CTAG 1 cut(s) 161
GluI GCNGC 2 cut(s) 55, 193
HaeIII GGCC 2 cut(s) 323, 466
HapII CCGG 7 cut(s) 216, 414, 1049, 1055, 1082, 1088, 1115
Hin1II CATG 7 cut(s) 149, 333, 344, 447, 888, 1215, 1239
HinfI GANTC 1 cut(s) 353
HpaII CCGG 7 cut(s) 216, 414, 1049, 1055, 1082, 1088, 1115
HphI GGTGA 1 cut(s) 426
Hpy188III TCNNGA 6 cut(s) 202, 256, 341, 712, 997, 1255
HpyAV CCTTC 4 cut(s) 227, 280, 459, 655
HpyCH4III ACNGT 3 cut(s) 85, 119, 386
HpyCH4V TGCA 3 cut(s) 180, 302, 317
HpyF10VI GCNNNNNNNGC 4 cut(s) 60, 1078, 1111, 1204
HpyF3I CTNAG 1 cut(s) 123
Hsp92II CATG 7 cut(s) 149, 333, 344, 447, 888, 1215, 1239
KroI GCCGGC 1 cut(s) 215
KroNI GCCGGC 1 cut(s) 217
Ksp22I TGATCA 1 cut(s) 73
Kzo9I GATC 6 cut(s) 6, 39, 73, 252, 699, 1281
LguI GCTCTTC 1 cut(s) 122
LmnI GCTCC 3 cut(s) 47, 212, 312
Lsp1109I GCAGC 1 cut(s) 66
MaeI CTAG 1 cut(s) 161
MaeIII GTNAC 3 cut(s) 166, 616, 658
MalI GATC 6 cut(s) 8, 41, 75, 254, 701, 1283
MboI GATC 6 cut(s) 6, 39, 73, 252, 699, 1281
MboII GAAGA 6 cut(s) 16, 16, 139, 476, 518, 919
MflI RGATCY 3 cut(s) 6, 699, 1281
MhlI GDGCHC 1 cut(s) 340
MluCI AATT 6 cut(s) 408, 428, 491, 533, 570, 591
MroNI GCCGGC 1 cut(s) 215
MroXI GAANNNNTTC 1 cut(s) 644
MseI TTAA 6 cut(s) 135, 474, 516, 558, 1302, 1348
MslI CAYNNNNRTG 2 cut(s) 362, 1234
MspI CCGG 7 cut(s) 216, 414, 1049, 1055, 1082, 1088, 1115
MwoI GCNNNNNNNGC 4 cut(s) 60, 1078, 1111, 1204
NaeI GCCGGC 1 cut(s) 217
NcoI CCATGG 2 cut(s) 1211, 1235
NdeII GATC 6 cut(s) 6, 39, 73, 252, 699, 1281
NgoMIV GCCGGC 1 cut(s) 215
NlaIII CATG 7 cut(s) 149, 333, 344, 447, 888, 1215, 1239
NlaIV GGNNCC 5 cut(s) 49, 214, 1053, 1086, 1119
NmuCI GTSAC 2 cut(s) 616, 658
NspI RCATGY 1 cut(s) 447
OliI CACNNNNGTG 1 cut(s) 362
PagI TCATGA 1 cut(s) 340
PciSI GCTCTTC 1 cut(s) 122
PdiI GCCGGC 1 cut(s) 217
PdmI GAANNNNTTC 1 cut(s) 644
PfeI GAWTC 1 cut(s) 353
PkrI GCNGC 2 cut(s) 56, 194
PspN4I GGNNCC 5 cut(s) 49, 214, 1053, 1086, 1119
PstNI CAGNNNCTG 2 cut(s) 992, 1022
PsuI RGATCY 3 cut(s) 6, 699, 1281
RsaI GTAC 8 cut(s) 112, 418, 1028, 1061, 1094, 1127, 1160, 1333
RsaNI GTAC 8 cut(s) 111, 417, 1027, 1060, 1093, 1126, 1159, 1332
RseI CAYNNNNRTG 2 cut(s) 362, 1234
SapI GCTCTTC 1 cut(s) 122
SaqAI TTAA 6 cut(s) 135, 474, 516, 558, 1302, 1348
SatI GCNGC 2 cut(s) 55, 193
Sau3AI GATC 6 cut(s) 6, 39, 73, 252, 699, 1281
ScaI AGTACT 1 cut(s) 1333
SduI GDGCHC 1 cut(s) 340
SmiMI CAYNNNNRTG 2 cut(s) 362, 1234
Sse9I AATT 6 cut(s) 408, 428, 491, 533, 570, 591
SsiI CCGC 8 cut(s) 192, 1046, 1070, 1079, 1103, 1112, 1136, 1205
SspMI CTAG 1 cut(s) 161
StyI CCWWGG 5 cut(s) 612, 654, 1211, 1235, 1341
TaaI ACNGT 3 cut(s) 85, 119, 386
TaqI TCGA 3 cut(s) 174, 201, 251
TaqII GACCGA 1 cut(s) 200
TasI AATT 6 cut(s) 408, 428, 491, 533, 570, 591
TatI WGTACW 6 cut(s) 1026, 1059, 1092, 1125, 1158, 1331
TauI GCSGC 1 cut(s) 195
TfiI GAWTC 1 cut(s) 353
Tru1I TTAA 6 cut(s) 135, 474, 516, 558, 1302, 1348
Tru9I TTAA 6 cut(s) 135, 474, 516, 558, 1302, 1348
TscAI CASTG 4 cut(s) 391, 909, 1161, 1176
TseFI GTSAC 2 cut(s) 616, 658
TseI GCWGC 1 cut(s) 54
Tsp45I GTSAC 2 cut(s) 616, 658
TspDTI ATGAA 3 cut(s) 17, 134, 361
TspRI CASTG 4 cut(s) 391, 909, 1161, 1176
XapI RAATTY 2 cut(s) 428, 591
XceI RCATGY 1 cut(s) 447
XmiI GTMKAC 3 cut(s) 807, 840, 873
XmnI GAANNNNTTC 1 cut(s) 644
XspI CTAG 1 cut(s) 161
ZrmI AGTACT 1 cut(s) 1333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.