Rh6BG023600

Proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0010868
Physical Location & Seq
Reverse (-)
3445938 .. 3449545
3608 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG023600.1

Sequence Viewer

Length: 1731 bp
ATGAAGATCTCTCGTCTGTGCCTCTTCTTGTCCTTGCTGCTTTTCGGAGCGAGCAGCTTTTGCTATGCTGATAATCATAAGACAGTTGAGGTAGTCGGGTTTGGAGAATGTACTGACTGTGCTGAGAAGAGCATTAAGACCAGTCATGCTTTTTCAGGTCTTGGTGTTACGATCGACTGCAAGGCCGAAAGCGGCCACTTCGAGAGGAGAGGGGCCGGCGAGCTCAATGAGGAGGGCAAGTTTTCAGTGTCCATCCCAAAGGAGATTGTGAAAGATGGAGGAGATGGACTGAAGGAGGAGTGCTATGCACAGCTCCACAATGCAAAGGCCACACCATGTGCTGCTCATGATGGACTTGAATCCACCAAAGTGGTATTCAAGTCTAAAAACAGTGATGGGAAACAAACCTTTGGAATTGTTGGTACTAAACTGAAGTTTTCACCATCTACATGCACTTCTGCTTTCTTTTGGCCTCCCTATAAACACCCACTTCCCAAATGGACCAAACCACATCCTCTCTTTGGACACCCAATATTCCCTTTCCCTCCAAAAAAATTCCCTCCATTCCCACCTAAGATTACTATTCCTCCATTCTATAAGAAGCCCTGCCCTCCCAAGATCCCTTTTTACGGAAAGCCCCTCCCCCCGCCAGTCCCAATTTACGAAAAGCCCCTCCCCCCACCAGTCCCAATCTACAAGAAGCCACTCCCTCCCCCAGTCCCAATATACAAGAAGCCACTCCCTCCCCCAGTACCAATATACAAGAAGCCGCTCCCTCCCCCAGTCCCAATATACAAGAAGCCGCTCCCTCCCCCAGTCCCAATATACAAGAAGCCCCTCCCTCCACCAGTCCCAATTTACAAGCCTCCACCAGTGCCGGTTTACAAGAAACCAAACCCTCCACCAGTACCTATCTACAAGAAACCATGCCCTCCACCAGTTCCAGTCTACAAGAAACCACTCCCTCCACCAGTACCAGTTTACAAAAAGCCCCATCCCCCAAAAGCACCCAAAAAAATGTGCCCTCCACCAGTCCCAGTGTACAAGAAGCCACTCCCTCCACCAGTCCCTGTGTACAAGAAGCCACTCCCTCCACCAGTCCCTGTATACAAGAAGCCACTCCCTCCACCAGTCCCTGTATACAAAAAGCCACTCCCACCACCAGTCCCTGTGTATAAAAAGCCACTCCCACCACCAGTCCCTGTGTATAAAAAGCCACTCCCACCACCAGTTCCAGTTTACAAAAAGCCACTCCCACCACCTGTTCCAGTGTACACAAAACCGCTTCCACCGCCGGTTCCTGTGTACACAAAACCGCTCCCACCACCGGTCCCAGTGTACACAAAACCACTCCCACCTCCAATCCCATTTTACAAACCAAAGCCACACCCATTTTACAAACCAAAACCACTTCCACCTCCAATTCCATTTTACAAACCAAAGCCACACCCATGGTTCAAGCCAAAGCCACTTCCACCTCCAATTCCATTTTACAAACCAAAGCCACACCCATGGTTCAAGCCAAAGCCACACCCATTTTACAAACCAAAACCACTTCCACCTCCAATCCCATTTTACAAACCAAAGCCACACCCATGGTTCAAGCCAAAGCCACACCCATTTTTCAAGCACCCATTCTTGAAGCCTCTACCTCCATTCCCCAAGATTCCTCATCATCCTTTCTTTAAGAAACCACCACCATTCCCACTACAAACATCTCCCAAGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

576

Amino Acids

64.72

Weight (kDa)

10.01

Isoelectric Point (pI)

65.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 31 - 116 6.6e-10 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010868)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21140 AT4G38770
fragaria_vesca FvH4_2g01970
malus_domestica MD05G1116400.v1.1 MD10G1118600.v1.1
prunus_persica Prupe.8G160900_v2.0.a1
pyrus_communis pycom05g11060 pycom10g10270
rosa_chinensis RchiOBHm_Chr6g0245161
rosa_laevigata RLG00000015320
rosa_multiflora Rmu_sc0001288.1_g000002 Rmu_sc0015012.1_g000001 Rmu_ssc0000087.1_g000001
rosa_roxburghii Rroxscaffold_7G00215400
rosa_rugosa Rorug05G0512400
rosa_samantha Rh6AG027500 Rh6BG023600 Rh6CG022000 Rh6DG022100
rosa_wichuraiana Rw6G002320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 3 cut(s) 772, 805, 1318
AccI GTMKAC 3 cut(s) 948, 1107, 1140
AciI CCGC 7 cut(s) 192, 647, 770, 803, 1283, 1292, 1316
AclWI GGATC 1 cut(s) 613
AcoI YGGCCR 1 cut(s) 193
AcsI RAATTY 1 cut(s) 554
AcuI CTGAAG 2 cut(s) 311, 452
AdeI CACNNNGTG 1 cut(s) 338
AfiI CCNNNNNNNGG 3 cut(s) 521, 629, 1327
AgeI ACCGGT 1 cut(s) 1327
AgsI TTSAA 7 cut(s) 359, 379, 1459, 1519, 1603, 1627, 1642
AjuI GAANNNNNNNTTGG 2 cut(s) 359, 391
AleI CACNNNNGTG 1 cut(s) 368
AluBI AGCT 3 cut(s) 57, 223, 313
AluI AGCT 3 cut(s) 57, 223, 313
Alw21I GWGCWC 1 cut(s) 225
AlwI GGATC 1 cut(s) 613
AlwNI CAGNNNCTG 5 cut(s) 1070, 1103, 1136, 1169, 1202
AoxI GGCC 5 cut(s) 183, 193, 213, 327, 470
ApeKI GCWGC 3 cut(s) 37, 54, 341
ApoI RAATTY 1 cut(s) 554
AsiGI ACCGGT 1 cut(s) 1327
AspS9I GGNCC 3 cut(s) 213, 501, 1330
AsuHPI GGTGA 1 cut(s) 432
AvaII GGWCC 2 cut(s) 501, 1330
BaeGI GKGCMC 1 cut(s) 1025
BanII GRGCYC 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 3 cut(s) 24, 66, 328
BccI CCATC 7 cut(s) 260, 269, 278, 344, 389, 451, 1002
BglII AGATCT 1 cut(s) 6
BisI GCNGC 6 cut(s) 38, 55, 193, 342, 770, 803
BlsI GCNGC 6 cut(s) 39, 56, 194, 343, 771, 804
Bme18I GGWCC 2 cut(s) 501, 1330
BmgT120I GGNCC 3 cut(s) 213, 501, 1330
BmiI GGNNCC 3 cut(s) 214, 1299, 1332
BmrI ACTGGG 6 cut(s) 710, 743, 776, 809, 1031, 1328
BmuI ACTGGG 6 cut(s) 710, 743, 776, 809, 1031, 1328
BsaJI CCNNGG 4 cut(s) 1451, 1511, 1595, 1722
BsaWI WCCGGW 1 cut(s) 1327
BsaXI ACNNNNNCTCC 4 cut(s) 224, 254, 571, 601
Bsc4I CCNNNNNNNGG 3 cut(s) 521, 629, 1327
Bse118I RCCGGY 4 cut(s) 215, 877, 1294, 1327
BseDI CCNNGG 4 cut(s) 1451, 1511, 1595, 1722
BseGI GGATG 4 cut(s) 252, 511, 994, 1675
BseLI CCNNNNNNNGG 3 cut(s) 521, 629, 1327
BseMII CTCAG 1 cut(s) 114
BseRI GAGGAG 4 cut(s) 220, 245, 294, 311
BseSI GKGCMC 1 cut(s) 1025
BseXI GCAGC 3 cut(s) 24, 66, 328
Bsh1285I CGRYCG 1 cut(s) 174
BshFI GGCC 5 cut(s) 185, 195, 215, 329, 472
BshTI ACCGGT 1 cut(s) 1327
BsiEI CGRYCG 1 cut(s) 174
BsiHKAI GWGCWC 1 cut(s) 225
BsiSI CCGG 4 cut(s) 216, 878, 1295, 1328
BslI CCNNNNNNNGG 3 cut(s) 521, 629, 1327
BsnI GGCC 5 cut(s) 185, 195, 215, 329, 472
Bsp1286I GDGCHC 2 cut(s) 225, 1025
Bsp1407I TGTACA 5 cut(s) 1041, 1074, 1272, 1305, 1338
Bsp143I GATC 3 cut(s) 6, 171, 618
Bsp19I CCATGG 3 cut(s) 1451, 1511, 1595
BspACI CCGC 7 cut(s) 192, 647, 770, 803, 1283, 1292, 1316
BspANI GGCC 5 cut(s) 185, 195, 215, 329, 472
BspCNI CTCAG 1 cut(s) 115
BspHI TCATGA 1 cut(s) 346
BspLI GGNNCC 3 cut(s) 214, 1299, 1332
BspPI GGATC 1 cut(s) 613
BspQI GCTCTTC 1 cut(s) 122
BsrBI CCGCTC 3 cut(s) 772, 805, 1318
BsrFI RCCGGY 4 cut(s) 215, 877, 1294, 1327
BsrGI TGTACA 5 cut(s) 1041, 1074, 1272, 1305, 1338
BssAI RCCGGY 4 cut(s) 215, 877, 1294, 1327
BssECI CCNNGG 4 cut(s) 1451, 1511, 1595, 1722
BssMI GATC 3 cut(s) 6, 171, 618
BssNAI GTATAC 2 cut(s) 1108, 1141
BssT1I CCWWGG 4 cut(s) 1451, 1511, 1595, 1722
Bst1107I GTATAC 2 cut(s) 1108, 1141
Bst4CI ACNGT 3 cut(s) 85, 119, 392
Bst6I CTCTTC 2 cut(s) 29, 122
BstAPI GCANNNNNTGC 1 cut(s) 60
BstAUI TGTACA 5 cut(s) 1041, 1074, 1272, 1305, 1338
BstC8I GCNNGC 3 cut(s) 52, 217, 221
BstDEI CTNAG 2 cut(s) 123, 573
BstDSI CCRYGG 3 cut(s) 1451, 1511, 1595
BstF5I GGATG 4 cut(s) 252, 511, 994, 1675
BstKTI GATC 3 cut(s) 9, 174, 621
BstMBI GATC 3 cut(s) 6, 171, 618
BstMCI CGRYCG 1 cut(s) 174
BstMWI GCNNNNNNNGC 2 cut(s) 60, 1291
BstNSI RCATGY 1 cut(s) 453
BstSLI GKGCMC 1 cut(s) 1025
BstV1I GCAGC 3 cut(s) 24, 66, 328
BstX2I RGATCY 2 cut(s) 6, 618
BstXI CCANNNNNNTGG 4 cut(s) 370, 1452, 1512, 1596
BstYI RGATCY 2 cut(s) 6, 618
BstZ17I GTATAC 2 cut(s) 1108, 1141
BsuRI GGCC 5 cut(s) 185, 195, 215, 329, 472
BtgI CCRYGG 3 cut(s) 1451, 1511, 1595
BtsCI GGATG 4 cut(s) 252, 511, 994, 1675
BtsIMutI CAGTG 6 cut(s) 252, 397, 879, 1044, 1275, 1341
Cac8I GCNNGC 3 cut(s) 52, 217, 221
CaiI CAGNNNCTG 5 cut(s) 1070, 1103, 1136, 1169, 1202
CciI TCATGA 1 cut(s) 346
Cfr10I RCCGGY 4 cut(s) 215, 877, 1294, 1327
Cfr13I GGNCC 3 cut(s) 213, 501, 1330
CspAI ACCGGT 1 cut(s) 1327
CviAII CATG 8 cut(s) 146, 336, 347, 450, 927, 1452, 1512, 1596
DdeI CTNAG 2 cut(s) 123, 573
DpnI GATC 3 cut(s) 8, 173, 620
DpnII GATC 3 cut(s) 6, 171, 618
DraIII CACNNNGTG 1 cut(s) 338
EaeI YGGCCR 1 cut(s) 193
Eam1104I CTCTTC 2 cut(s) 29, 122
EarI CTCTTC 2 cut(s) 29, 122
Ecl136II GAGCTC 1 cut(s) 223
Eco130I CCWWGG 4 cut(s) 1451, 1511, 1595, 1722
Eco24I GRGCYC 1 cut(s) 225
Eco47I GGWCC 2 cut(s) 501, 1330
Eco53kI GAGCTC 1 cut(s) 223
Eco57I CTGAAG 2 cut(s) 311, 452
EcoICRI GAGCTC 1 cut(s) 223
EcoT14I CCWWGG 4 cut(s) 1451, 1511, 1595, 1722
EcoT38I GRGCYC 1 cut(s) 225
ErhI CCWWGG 4 cut(s) 1451, 1511, 1595, 1722
FaeI CATG 8 cut(s) 149, 339, 350, 453, 930, 1455, 1515, 1599
FatI CATG 8 cut(s) 145, 335, 346, 449, 926, 1451, 1511, 1595
FauI CCCGC 1 cut(s) 654
FblI GTMKAC 3 cut(s) 948, 1107, 1140
Fnu4HI GCNGC 6 cut(s) 38, 55, 193, 342, 770, 803
FokI GGATG 4 cut(s) 239, 498, 981, 1662
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 6 cut(s) 38, 55, 193, 342, 770, 803
GluI GCNGC 6 cut(s) 38, 55, 193, 342, 770, 803
HaeIII GGCC 5 cut(s) 185, 195, 215, 329, 472
HapII CCGG 4 cut(s) 216, 878, 1295, 1328
Hin1II CATG 8 cut(s) 149, 339, 350, 453, 930, 1455, 1515, 1599
HinfI GANTC 2 cut(s) 359, 1666
HpaII CCGG 4 cut(s) 216, 878, 1295, 1328
HphI GGTGA 1 cut(s) 432
Hpy188I TCNGA 1 cut(s) 47
Hpy188III TCNNGA 3 cut(s) 202, 347, 1639
HpyAV CCTTC 1 cut(s) 286
HpyCH4III ACNGT 3 cut(s) 85, 119, 392
HpyCH4V TGCA 4 cut(s) 180, 308, 323, 453
HpyF10VI GCNNNNNNNGC 2 cut(s) 60, 1291
HpyF3I CTNAG 2 cut(s) 123, 573
Hsp92II CATG 8 cut(s) 149, 339, 350, 453, 930, 1455, 1515, 1599
KroI GCCGGC 1 cut(s) 215
KroNI GCCGGC 1 cut(s) 217
Kzo9I GATC 3 cut(s) 6, 171, 618
LguI GCTCTTC 1 cut(s) 122
LmnI GCTCC 5 cut(s) 47, 318, 777, 810, 1323
Lsp1109I GCAGC 3 cut(s) 24, 66, 328
MaeIII GTNAC 1 cut(s) 166
MalI GATC 3 cut(s) 8, 173, 620
MbiI CCGCTC 3 cut(s) 772, 805, 1318
MboI GATC 3 cut(s) 6, 171, 618
MboII GAAGA 3 cut(s) 16, 16, 139
MflI RGATCY 2 cut(s) 6, 618
MhlI GDGCHC 2 cut(s) 225, 1025
MluCI AATT 6 cut(s) 414, 554, 657, 855, 1422, 1482
MroNI GCCGGC 1 cut(s) 215
MseI TTAA 3 cut(s) 135, 1686, 1729
MslI CAYNNNNRTG 5 cut(s) 368, 448, 1450, 1510, 1594
MspI CCGG 4 cut(s) 216, 878, 1295, 1328
MwoI GCNNNNNNNGC 2 cut(s) 60, 1291
NaeI GCCGGC 1 cut(s) 217
NcoI CCATGG 3 cut(s) 1451, 1511, 1595
NdeII GATC 3 cut(s) 6, 171, 618
NgoMIV GCCGGC 1 cut(s) 215
NlaIII CATG 8 cut(s) 149, 339, 350, 453, 930, 1455, 1515, 1599
NlaIV GGNNCC 3 cut(s) 214, 1299, 1332
NspI RCATGY 1 cut(s) 453
OliI CACNNNNGTG 1 cut(s) 368
PagI TCATGA 1 cut(s) 346
PciSI GCTCTTC 1 cut(s) 122
PdiI GCCGGC 1 cut(s) 217
PfeI GAWTC 2 cut(s) 359, 1666
PinAI ACCGGT 1 cut(s) 1327
PkrI GCNGC 6 cut(s) 39, 56, 194, 343, 771, 804
Ple19I CGATCG 1 cut(s) 174
Psp124BI GAGCTC 1 cut(s) 225
PspN4I GGNNCC 3 cut(s) 214, 1299, 1332
PspPI GGNCC 3 cut(s) 213, 501, 1330
PstNI CAGNNNCTG 5 cut(s) 1070, 1103, 1136, 1169, 1202
PsuI RGATCY 2 cut(s) 6, 618
PvuI CGATCG 1 cut(s) 174
RseI CAYNNNNRTG 5 cut(s) 368, 448, 1450, 1510, 1594
SacI GAGCTC 1 cut(s) 225
SapI GCTCTTC 1 cut(s) 122
SaqAI TTAA 3 cut(s) 135, 1686, 1729
SatI GCNGC 6 cut(s) 38, 55, 193, 342, 770, 803
Sau3AI GATC 3 cut(s) 6, 171, 618
Sau96I GGNCC 3 cut(s) 213, 501, 1330
SduI GDGCHC 2 cut(s) 225, 1025
SinI GGWCC 2 cut(s) 501, 1330
SmiMI CAYNNNNRTG 5 cut(s) 368, 448, 1450, 1510, 1594
Sse9I AATT 6 cut(s) 414, 554, 657, 855, 1422, 1482
SsiI CCGC 7 cut(s) 192, 647, 770, 803, 1283, 1292, 1316
SspI AATATT 1 cut(s) 534
SstI GAGCTC 1 cut(s) 225
StyI CCWWGG 4 cut(s) 1451, 1511, 1595, 1722
TaaI ACNGT 3 cut(s) 85, 119, 392
TaqI TCGA 2 cut(s) 174, 201
TasI AATT 6 cut(s) 414, 554, 657, 855, 1422, 1482
TatI WGTACW 6 cut(s) 110, 1041, 1074, 1272, 1305, 1338
TauI GCSGC 3 cut(s) 195, 772, 805
TfiI GAWTC 2 cut(s) 359, 1666
Tru1I TTAA 3 cut(s) 135, 1686, 1729
Tru9I TTAA 3 cut(s) 135, 1686, 1729
TscAI CASTG 6 cut(s) 252, 397, 879, 1044, 1275, 1341
TseI GCWGC 3 cut(s) 37, 54, 341
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 645
TspRI CASTG 6 cut(s) 252, 397, 879, 1044, 1275, 1341
VpaK11BI GGWCC 2 cut(s) 501, 1330
XapI RAATTY 1 cut(s) 554
XceI RCATGY 1 cut(s) 453
XcmI CCANNNNNNNNNTGG 1 cut(s) 495
XmiI GTMKAC 3 cut(s) 948, 1107, 1140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.