MD05G1116400.v1.1

Proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010868
Physical Location & Seq
Forward (+)
23731857 .. 23734264
2408 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1116400.v1.1.491

Sequence Viewer

Length: 1425 bp
ATGAAGATTCTCCAGAAGTGCTTCTGGCTGTGTCTGCTTTTTGTGAGCTTTAGCCATGCTGAGCAGACAGTTGAGGTAGTAGGGGTTGGAGAATGTACTGACTGTGCTCGGAATAACATTAAGACTAGCCATGCCTTTTCTGGTCTTCGTGTAACAATTGACTGCAAGCCCGAAAATGGGCACTTCAAAACAAGAGGAGTTGGGGAGCTCAATGAACAGGGAGAGTTCAAAGTCTCACTTCCTAAAGAGATTGTGAAAGAAGAAAACCAATTAAAAGAGGAATGTTATGCCCAACTCCACAGTGCATTAGCTGCCCCTTGTGCTGCCCATGACGGCCTAAAATCCACGAAAATAGTCTTCAAGTCCAAGGCAAGTGATGGGACGCAAACCTTTGGAGTGGCTGGTGGAAATCTCAAATTTTCCCCTGTGACTTGCACTTCTGCCTTCTTTTGGCCTCACAAGAAGCACCCACTTTTTTCCAAATTGCCACCTTTGCCTAAGTTGCCACCTTTCCCTAAATCTCACCCAATTTTTGGACACCCATTCCCATTCCCTCCTAAGGTGTTACCTCCATTCCCACCTAAGGTCTTCCCTCCAAAAATTCCAATCTTCAAGAAGCCTCTTCCTCCACCAGTGCCAATCTACAAGAAGCCTCTCCCTCCACCAGTGCCGGTATACAAGAAGCCGGTCCCTCCCCCCGTCCCGGTCTACAAGAAGCCTCACCCTCCGCCCGTCCCAATCTACAAGAAACCTCTCCCTCCACCTGTCCCAGTCTATAAGAAGCCTAACCCTCCACCAGTCCCAGTATACAAGAAACCTTACCCTCCACCAACACCAATCTACAAGAAACCTCTCCCTCCTCCCGTCCCAGTCTATAAGAAGCCTAACCCTCCACCAGTCCCAGTATACAAGAAACCTTGCCCTCCACCAAAACCAATCTACAAGAAACCTCTCCCTCCACCTGTCCCAATCTATAAGAAGCCTCTTCCACCACCAGTTCCAGTATACAAGAAGCCACTTCCACCACTAGTCCCCATATACAAGAAGCCATTTCCACCACCAGTCCCAATATACACAAAGCCACTTCCACCACCAATCCCAATATACAAGAAGCCACTTCCACCACTAGTCCCATTATACAAGAAGCCACTTCCACCACTAGTCCCCATATACAAGAAGCCATTTCCACCACCAGTCCCAATATACACAAAGCCACTTCCACCACCAATCCCAATATACACAAAGCCACTTCCACCTCCCATCCCATTCTACAAGCCAAAGCCACATCCATTCTTTAAGCCTCTCCCTCCATTGCCAAAGATTCCTCATCCATTCTTTAAGAAACCACCACTCCCACCACTCCACCCAAAATATTACTTCCCCCACCCAAAGCCCAAGCTTCCTCTCATCCCCAAAATCCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

475

Amino Acids

53.14

Weight (kDa)

10.01

Isoelectric Point (pI)

57.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 26 - 110 1.8e-11 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010868)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21140 AT4G38770
fragaria_vesca FvH4_2g01970
malus_domestica MD05G1116400.v1.1 MD10G1118600.v1.1
prunus_persica Prupe.8G160900_v2.0.a1
pyrus_communis pycom05g11060 pycom10g10270
rosa_chinensis RchiOBHm_Chr6g0245161
rosa_laevigata RLG00000015320
rosa_multiflora Rmu_sc0001288.1_g000002 Rmu_sc0015012.1_g000001 Rmu_ssc0000087.1_g000001
rosa_roxburghii Rroxscaffold_7G00215400
rosa_rugosa Rorug05G0512400
rosa_samantha Rh6AG027500 Rh6BG023600 Rh6CG022000 Rh6DG022100
rosa_wichuraiana Rw6G002320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 533
AccI GTMKAC 5 cut(s) 675, 708, 807, 906, 1005
AciI CCGC 1 cut(s) 728
AcsI RAATTY 2 cut(s) 416, 600
AfaI GTAC 1 cut(s) 97
AfiI CCNNNNNNNGG 7 cut(s) 176, 177, 450, 533, 559, 583, 703
AgsI TTSAA 4 cut(s) 187, 229, 361, 613
AhlI ACTAGT 3 cut(s) 1027, 1126, 1159
AluBI AGCT 4 cut(s) 48, 208, 311, 1399
AluI AGCT 4 cut(s) 48, 208, 311, 1399
Alw21I GWGCWC 2 cut(s) 109, 210
Alw26I GTCTC 1 cut(s) 238
AoxI GGCC 2 cut(s) 334, 452
ApeKI GCWGC 2 cut(s) 311, 323
ApoI RAATTY 2 cut(s) 416, 600
Asp700I GAANNNNTTC 1 cut(s) 20
AspS9I GGNCC 1 cut(s) 688
AsuC2I CCSGG 1 cut(s) 704
AsuHPI GGTGA 2 cut(s) 515, 713
AvaII GGWCC 1 cut(s) 688
AxyI CCTNAGG 2 cut(s) 558, 582
BaeGI GKGCMC 1 cut(s) 183
BanII GRGCYC 1 cut(s) 210
BbsI GAAGAC 3 cut(s) 137, 349, 580
Bbv12I GWGCWC 2 cut(s) 109, 210
BbvI GCAGC 2 cut(s) 298, 310
BccI CCATC 2 cut(s) 371, 1268
BceAI ACGGC 1 cut(s) 349
BcnI CCSGG 1 cut(s) 704
BcoDI GTCTC 1 cut(s) 238
BcuI ACTAGT 3 cut(s) 1027, 1126, 1159
BfaI CTAG 4 cut(s) 126, 1028, 1127, 1160
BisI GCNGC 2 cut(s) 312, 324
BlpI GCTNAGC 1 cut(s) 60
BlsI GCNGC 2 cut(s) 313, 325
Bme1390I CCNGG 1 cut(s) 704
Bme18I GGWCC 1 cut(s) 688
BmgT120I GGNCC 1 cut(s) 688
BmiI GGNNCC 1 cut(s) 690
BmrFI CCNGG 1 cut(s) 704
BmrI ACTGGG 4 cut(s) 764, 797, 863, 896
BmuI ACTGGG 4 cut(s) 764, 797, 863, 896
BpiI GAAGAC 3 cut(s) 137, 349, 580
Bpu1102I GCTNAGC 1 cut(s) 60
BpuMI CCSGG 1 cut(s) 704
BsaJI CCNNGG 1 cut(s) 366
BsaXI ACNNNNNCTCC 2 cut(s) 1335, 1365
Bsc4I CCNNNNNNNGG 7 cut(s) 176, 177, 450, 533, 559, 583, 703
Bse118I RCCGGY 2 cut(s) 670, 685
Bse21I CCTNAGG 2 cut(s) 558, 582
Bse3DI GCAATG 1 cut(s) 1310
BseDI CCNNGG 1 cut(s) 366
BseGI GGATG 4 cut(s) 1260, 1285, 1327, 1407
BseLI CCNNNNNNNGG 7 cut(s) 176, 177, 450, 533, 559, 583, 703
BseMI GCAATG 1 cut(s) 1310
BseMII CTCAG 1 cut(s) 51
BseRI GAGGAG 2 cut(s) 210, 849
BseSI GKGCMC 1 cut(s) 183
BseXI GCAGC 2 cut(s) 298, 310
BshFI GGCC 2 cut(s) 336, 454
BsiHKAI GWGCWC 2 cut(s) 109, 210
BsiSI CCGG 3 cut(s) 671, 686, 704
BslI CCNNNNNNNGG 7 cut(s) 176, 177, 450, 533, 559, 583, 703
BsmAI GTCTC 1 cut(s) 238
BsnI GGCC 2 cut(s) 336, 454
Bsp1286I GDGCHC 3 cut(s) 109, 183, 210
Bsp1720I GCTNAGC 1 cut(s) 60
BspACI CCGC 1 cut(s) 728
BspANI GGCC 2 cut(s) 336, 454
BspCNI CTCAG 1 cut(s) 52
BspLI GGNNCC 1 cut(s) 690
BsrDI GCAATG 1 cut(s) 1310
BsrFI RCCGGY 2 cut(s) 670, 685
BssAI RCCGGY 2 cut(s) 670, 685
BssECI CCNNGG 1 cut(s) 366
BssNAI GTATAC 4 cut(s) 676, 808, 907, 1006
BssT1I CCWWGG 1 cut(s) 366
Bst1107I GTATAC 4 cut(s) 676, 808, 907, 1006
Bst4CI ACNGT 3 cut(s) 70, 104, 302
Bst6I CTCTTC 2 cut(s) 627, 990
BstAPI GCANNNNNTGC 1 cut(s) 311
BstC8I GCNNGC 1 cut(s) 167
BstDEI CTNAG 4 cut(s) 60, 498, 558, 582
BstF5I GGATG 4 cut(s) 1260, 1285, 1327, 1407
BstMAI GTCTC 1 cut(s) 238
BstMWI GCNNNNNNNGC 5 cut(s) 34, 311, 320, 493, 502
BstSCI CCNGG 1 cut(s) 702
BstSLI GKGCMC 1 cut(s) 183
BstV1I GCAGC 2 cut(s) 298, 310
BstV2I GAAGAC 3 cut(s) 137, 349, 580
BstZ17I GTATAC 4 cut(s) 676, 808, 907, 1006
Bsu36I CCTNAGG 2 cut(s) 558, 582
BsuRI GGCC 2 cut(s) 336, 454
BtsCI GGATG 4 cut(s) 1260, 1285, 1327, 1407
BtsIMutI CAGTG 3 cut(s) 307, 639, 672
Cac8I GCNNGC 1 cut(s) 167
Cfr10I RCCGGY 2 cut(s) 670, 685
Cfr13I GGNCC 1 cut(s) 688
CseI GACGC 1 cut(s) 391
Csp6I GTAC 1 cut(s) 96
CviAII CATG 3 cut(s) 56, 131, 329
CviQI GTAC 1 cut(s) 96
DdeI CTNAG 4 cut(s) 60, 498, 558, 582
Eam1104I CTCTTC 2 cut(s) 627, 990
EarI CTCTTC 2 cut(s) 627, 990
EciI GGCGGA 1 cut(s) 717
Ecl136II GAGCTC 1 cut(s) 208
Eco130I CCWWGG 1 cut(s) 366
Eco24I GRGCYC 1 cut(s) 210
Eco47I GGWCC 1 cut(s) 688
Eco53kI GAGCTC 1 cut(s) 208
Eco81I CCTNAGG 2 cut(s) 558, 582
EcoICRI GAGCTC 1 cut(s) 208
EcoT14I CCWWGG 1 cut(s) 366
EcoT38I GRGCYC 1 cut(s) 210
ErhI CCWWGG 1 cut(s) 366
FaeI CATG 3 cut(s) 59, 134, 332
FalI AAGNNNNNCTT 2 cut(s) 222, 254
FatI CATG 3 cut(s) 55, 130, 328
FblI GTMKAC 5 cut(s) 675, 708, 807, 906, 1005
Fnu4HI GCNGC 2 cut(s) 312, 324
FokI GGATG 4 cut(s) 1247, 1272, 1314, 1394
FriOI GRGCYC 1 cut(s) 210
Fsp4HI GCNGC 2 cut(s) 312, 324
FspBI CTAG 4 cut(s) 126, 1028, 1127, 1160
GluI GCNGC 2 cut(s) 312, 324
HaeIII GGCC 2 cut(s) 336, 454
HapII CCGG 3 cut(s) 671, 686, 704
HgaI GACGC 1 cut(s) 391
Hin1II CATG 3 cut(s) 59, 134, 332
HindIII AAGCTT 1 cut(s) 1397
HinfI GANTC 2 cut(s) 7, 1321
HpaII CCGG 3 cut(s) 671, 686, 704
HphI GGTGA 2 cut(s) 515, 713
Hpy166II GTNNAC 5 cut(s) 676, 709, 808, 907, 1006
Hpy188I TCNGA 1 cut(s) 111
Hpy188III TCNNGA 2 cut(s) 13, 613
Hpy8I GTNNAC 5 cut(s) 676, 709, 808, 907, 1006
HpyAV CCTTC 1 cut(s) 454
HpyCH4III ACNGT 3 cut(s) 70, 104, 302
HpyCH4V TGCA 3 cut(s) 165, 305, 435
HpyF10VI GCNNNNNNNGC 5 cut(s) 34, 311, 320, 493, 502
HpyF3I CTNAG 4 cut(s) 60, 498, 558, 582
Hsp92II CATG 3 cut(s) 59, 134, 332
LmnI GCTCC 1 cut(s) 205
Lsp1109I GCAGC 2 cut(s) 298, 310
MaeI CTAG 4 cut(s) 126, 1028, 1127, 1160
MaeIII GTNAC 3 cut(s) 151, 427, 564
MboII GAAGA 8 cut(s) 16, 137, 272, 349, 580, 601, 614, 977
MfeI CAATTG 1 cut(s) 156
MhlI GDGCHC 3 cut(s) 109, 183, 210
MluCI AATT 6 cut(s) 156, 269, 416, 482, 528, 600
MmeI TCCRAC 1 cut(s) 67
MroXI GAANNNNTTC 1 cut(s) 20
MseI TTAA 4 cut(s) 120, 272, 1296, 1338
MspI CCGG 3 cut(s) 671, 686, 704
MspR9I CCNGG 1 cut(s) 704
MunI CAATTG 1 cut(s) 156
MwoI GCNNNNNNNGC 5 cut(s) 34, 311, 320, 493, 502
NciI CCSGG 1 cut(s) 704
NlaIII CATG 3 cut(s) 59, 134, 332
NlaIV GGNNCC 1 cut(s) 690
NmuCI GTSAC 1 cut(s) 427
PdmI GAANNNNTTC 1 cut(s) 20
PfeI GAWTC 2 cut(s) 7, 1321
PflMI CCANNNNNTGG 1 cut(s) 533
PkrI GCNGC 2 cut(s) 313, 325
Psp124BI GAGCTC 1 cut(s) 210
PspN4I GGNNCC 1 cut(s) 690
PspPI GGNCC 1 cut(s) 688
RsaI GTAC 1 cut(s) 97
RsaNI GTAC 1 cut(s) 96
SacI GAGCTC 1 cut(s) 210
SaqAI TTAA 4 cut(s) 120, 272, 1296, 1338
SatI GCNGC 2 cut(s) 312, 324
Sau96I GGNCC 1 cut(s) 688
ScrFI CCNGG 1 cut(s) 704
SduI GDGCHC 3 cut(s) 109, 183, 210
SinI GGWCC 1 cut(s) 688
SpeI ACTAGT 3 cut(s) 1027, 1126, 1159
Sse9I AATT 6 cut(s) 156, 269, 416, 482, 528, 600
SsiI CCGC 1 cut(s) 728
SspI AATATT 1 cut(s) 1373
SspMI CTAG 4 cut(s) 126, 1028, 1127, 1160
SstI GAGCTC 1 cut(s) 210
StyD4I CCNGG 1 cut(s) 702
StyI CCWWGG 1 cut(s) 366
TaaI ACNGT 3 cut(s) 70, 104, 302
TasI AATT 6 cut(s) 156, 269, 416, 482, 528, 600
TatI WGTACW 1 cut(s) 95
TfiI GAWTC 2 cut(s) 7, 1321
Tru1I TTAA 4 cut(s) 120, 272, 1296, 1338
Tru9I TTAA 4 cut(s) 120, 272, 1296, 1338
TscAI CASTG 3 cut(s) 307, 639, 672
TseFI GTSAC 1 cut(s) 427
TseI GCWGC 2 cut(s) 311, 323
Tsp45I GTSAC 1 cut(s) 427
TspDTI ATGAA 2 cut(s) 17, 228
TspRI CASTG 3 cut(s) 307, 639, 672
Van91I CCANNNNNTGG 1 cut(s) 533
VpaK11BI GGWCC 1 cut(s) 688
XapI RAATTY 2 cut(s) 416, 600
XcmI CCANNNNNNNNNTGG 1 cut(s) 137
XmiI GTMKAC 5 cut(s) 675, 708, 807, 906, 1005
XmnI GAANNNNTTC 1 cut(s) 20
XspI CTAG 4 cut(s) 126, 1028, 1127, 1160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.