Rmu_ssc0000087.1_g000001

Proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000087.1
OG0010868
Physical Location & Seq
Forward (+)
699 .. 9117
8419 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000087.1_g000001.1.cds

Sequence Viewer

Length: 2112 bp
atgaagatctctcatctgtgcctcttcttgtccttgctgcttttcggagcgagcagcttttgctatgctgatgatcataagacagttgaggtagtcgggtttggagaatgcactgactgtgctgagaagagcattaagaccagtcatgctttttcaggtcttggtgttacgatcgactgcaaggccgaaagcggcctcttcgagaggagaggggccggcgagctcaatgaggagggcaagttttcagtgtccatcccaaaggagattgtgaaagatggaggagatggactgaaggaggagtgctatgcacagctccacaatgcaaaggccacaccatgcgctgctcatgatggacttgaatccaccaaagtggtattcaagtctaaaaacagtgatgggaaacaaacctttggaattgttggtactaaactaaagttttcaccatctacatgcacttctgctttcttttggcctccctttaagcacccacttcacaaatggaccaaaccacatcctctctttggacacccaatattccctttccctccaaaaaaattccctccattcccacctaaacctaagattactattcctccattctataagaagccctgccctcccaagatcccaatttacgaaaagcccctccccccgccagtcccaatttacgaaaagcccctccccccaccagtcccaatctacaagaagccgctccctcccccatcgcctttcttagtaaactgtcttgcttctctccgacgtatgagtacgatatgggggctggtaagggaagtatcaaaaggagtggtgggtttttgggagaagatggaagaggtggtgaatggtctgtctgccaaactggtaatttcagaggcggaggactttaaggtggttaagattgacgatgtggaggatatgtctctaaagaagtactggttagtggggagacttctaacttcgaaacctttcaacaaggagtcacttcggagtactatgaagaggatctggcatattaaaggtgaggtttcaatttttgaatgggagaattctggcaggtttgttttttcttttcagtttgatgccgataggaggaaaatccttagaggcatcccatggacttttgataatgccttattgttgttctcggctatggatatgaaatctgatcctttgaaagtacctttgatgagaaaattggtggacgttgaccctggggtgaatggagactgctctgcgagctttctcttaatccgagtggggttggatgtcaatgtgcctctaagaagatggattaggcttcaacttgagaattcaggagaggaagtcaaactcttactggattacgagaaccttccctacttttgtttctattgtggttgtttggatcatgttagctcttgctgtgaattgaaaaaggcggggaaaatacaggaagtgcagccaagagatgagtttctaggaagcggttgtggtctgaatagtgaaatggtggtggatggtgagttggcaggggatcaccttgaggcatcggaggcaaagcggaggtggatggcggtccctcctccttctttagacgtgcctgataatatgaaggtaattgaggaaagagaaattattacgggaaagaatattggaggatccaatcagtcattattacaaaagcattcaccgcagtctcaggcggggttggagaatattcataccctaaatcgtcacttgagatccgacatcctctcagctttgatttttgaagatttacagattggcgggacgttaggaggcgtaagtttaagagctgcttcaaatttgaagtcggggggtgttaatgggcctgatgagctggcaagtcctttagtaaaacaacaggaaatttggcccattttgaaacttgagggggtcaataggaatcacaggttggtggaggactctcaggttgtggagcaaagtggggatgcagtggtttataagagagttggaaagaaaaagggagagaagaaagaagcgttaggaaagcaggcagcgtcaaagggggagaatgaaggtcatggaggtcgggttggcttcttcgtgggaggtcgggggagacgaaggagtaaggcttctcctaattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

703

Amino Acids

78.02

Weight (kDa)

8.89

Isoelectric Point (pI)

50.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010868)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21140 AT4G38770
fragaria_vesca FvH4_2g01970
malus_domestica MD05G1116400.v1.1 MD10G1118600.v1.1
prunus_persica Prupe.8G160900_v2.0.a1
pyrus_communis pycom05g11060 pycom10g10270
rosa_chinensis RchiOBHm_Chr6g0245161
rosa_laevigata RLG00000015320
rosa_multiflora Rmu_sc0001288.1_g000002 Rmu_sc0015012.1_g000001 Rmu_ssc0000087.1_g000001
rosa_roxburghii Rroxscaffold_7G00215400
rosa_rugosa Rorug05G0512400
rosa_samantha Rh6AG027500 Rh6BG023600 Rh6CG022000 Rh6DG022100
rosa_wichuraiana Rw6G002320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1965
Acc36I ACCTGC 1 cut(s) 1044
AccBSI CCGCTC 1 cut(s) 712
AclWI GGATC 8 cut(s) 619, 1010, 1160, 1392, 1521, 1632, 1645, 1716
AcsI RAATTY 5 cut(s) 554, 1045, 1309, 1804, 1869
AcuI CTGAAG 1 cut(s) 311
AfaI GTAC 5 cut(s) 424, 769, 932, 991, 1179
AfiI CCNNNNNNNGG 2 cut(s) 521, 1089
AjiI CACGTC 1 cut(s) 1576
AjnI CCWGG 1 cut(s) 1210
AjuI GAANNNNNNNTTGG 4 cut(s) 359, 391, 1898, 1930
AleI CACNNNNGTG 1 cut(s) 368
AluBI AGCT 8 cut(s) 57, 223, 313, 1239, 1395, 1739, 1796, 1840
AluI AGCT 8 cut(s) 57, 223, 313, 1239, 1395, 1739, 1796, 1840
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 5 cut(s) 924, 940, 1218, 1680, 2077
AlwI GGATC 8 cut(s) 619, 1010, 1160, 1392, 1521, 1632, 1645, 1716
AoxI GGCC 7 cut(s) 183, 193, 213, 327, 470, 1829, 1874
ApeKI GCWGC 6 cut(s) 37, 54, 341, 1438, 1796, 2018
ApoI RAATTY 5 cut(s) 554, 1045, 1309, 1804, 1869
ArsI GACNNNNNNTTYG 2 cut(s) 1796, 1828
Asp700I GAANNNNTTC 1 cut(s) 965
AspLEI GCGC 1 cut(s) 341
AspS9I GGNCC 5 cut(s) 213, 501, 1555, 1829, 1875
AsuHPI GGTGA 7 cut(s) 432, 850, 1031, 1228, 1508, 1511, 1659
AsuII TTCGAA 1 cut(s) 959
AvaII GGWCC 2 cut(s) 501, 1555
BamHI GGATCC 1 cut(s) 1637
BanII GRGCYC 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 6 cut(s) 24, 66, 328, 1450, 1783, 2030
BciT130I CCWGG 1 cut(s) 1212
BclI TGATCA 1 cut(s) 73
BcoDI GTCTC 5 cut(s) 924, 940, 1218, 1680, 2077
BfaI CTAG 1 cut(s) 1457
BfuAI ACCTGC 1 cut(s) 1044
BglII AGATCT 1 cut(s) 6
BisI GCNGC 8 cut(s) 38, 55, 193, 342, 710, 1439, 1797, 2019
BlsI GCNGC 8 cut(s) 39, 56, 194, 343, 711, 1440, 1798, 2020
BmcAI AGTACT 2 cut(s) 932, 991
Bme1390I CCNGG 1 cut(s) 1212
Bme18I GGWCC 2 cut(s) 501, 1555
BmgBI CACGTC 1 cut(s) 1576
BmgT120I GGNCC 5 cut(s) 213, 501, 1555, 1829, 1875
BmiI GGNNCC 3 cut(s) 214, 1557, 1639
BmrFI CCNGG 1 cut(s) 1212
BmsI GCATC 4 cut(s) 1069, 1116, 1535, 1942
Bpu14I TTCGAA 1 cut(s) 959
BpuEI CTTGAG 4 cut(s) 1325, 1541, 1738, 1910
BsaJI CCNNGG 3 cut(s) 1112, 1210, 1211
Bsc4I CCNNNNNNNGG 2 cut(s) 521, 1089
Bse118I RCCGGY 1 cut(s) 215
Bse1I ACTGG 6 cut(s) 141, 656, 689, 864, 938, 1341
BseBI CCWGG 1 cut(s) 1212
BseDI CCNNGG 3 cut(s) 1112, 1210, 1211
BseGI GGATG 8 cut(s) 252, 511, 1107, 1270, 1501, 1554, 1728, 1957
BseLI CCNNNNNNNGG 2 cut(s) 521, 1089
BseMII CTCAG 4 cut(s) 114, 1691, 1749, 1943
BseNI ACTGG 6 cut(s) 141, 656, 689, 864, 938, 1341
BseRI GAGGAG 5 cut(s) 220, 245, 294, 311, 1551
BseXI GCAGC 6 cut(s) 24, 66, 328, 1450, 1783, 2030
BsgI GTGCAG 1 cut(s) 1457
Bsh1285I CGRYCG 1 cut(s) 174
BshFI GGCC 7 cut(s) 185, 195, 215, 329, 472, 1831, 1876
BsiEI CGRYCG 1 cut(s) 174
BsiHKAI GWGCWC 1 cut(s) 225
BsiSI CCGG 1 cut(s) 216
BslFI GGGAC 4 cut(s) 644, 677, 1541, 1783
BslI CCNNNNNNNGG 2 cut(s) 521, 1089
BsmAI GTCTC 5 cut(s) 924, 940, 1218, 1680, 2077
BsmBI CGTCTC 1 cut(s) 2077
BsmFI GGGAC 4 cut(s) 644, 677, 1541, 1783
BsmI GAATGC 2 cut(s) 113, 1663
BsnI GGCC 7 cut(s) 185, 195, 215, 329, 472, 1831, 1876
Bsp119I TTCGAA 1 cut(s) 959
Bsp1286I GDGCHC 1 cut(s) 225
Bsp19I CCATGG 1 cut(s) 1112
BspANI GGCC 7 cut(s) 185, 195, 215, 329, 472, 1831, 1876
BspCNI CTCAG 4 cut(s) 115, 1690, 1748, 1942
BspHI TCATGA 1 cut(s) 346
BspLI GGNNCC 3 cut(s) 214, 1557, 1639
BspMI ACCTGC 1 cut(s) 1044
BspPI GGATC 8 cut(s) 619, 1010, 1160, 1392, 1521, 1632, 1645, 1716
BspQI GCTCTTC 1 cut(s) 122
BspT104I TTCGAA 1 cut(s) 959
BsrBI CCGCTC 1 cut(s) 712
BsrFI RCCGGY 1 cut(s) 215
BsrI ACTGG 6 cut(s) 141, 656, 689, 864, 938, 1341
BssAI RCCGGY 1 cut(s) 215
BssECI CCNNGG 3 cut(s) 1112, 1210, 1211
BssT1I CCWWGG 1 cut(s) 1112
Bst2UI CCWGG 1 cut(s) 1212
Bst4CI ACNGT 4 cut(s) 85, 119, 392, 743
Bst6I CTCTTC 5 cut(s) 29, 122, 203, 825, 992
BstAPI GCANNNNNTGC 1 cut(s) 60
BstBI TTCGAA 1 cut(s) 959
BstC8I GCNNGC 6 cut(s) 52, 217, 221, 1237, 1842, 2016
BstDEI CTNAG 8 cut(s) 123, 579, 733, 1100, 1280, 1677, 1735, 1929
BstDSI CCRYGG 1 cut(s) 1112
BstF5I GGATG 8 cut(s) 252, 511, 1107, 1270, 1501, 1554, 1728, 1957
BstHHI GCGC 1 cut(s) 341
BstMAI GTCTC 5 cut(s) 924, 940, 1218, 1680, 2077
BstMCI CGRYCG 1 cut(s) 174
BstMWI GCNNNNNNNGC 5 cut(s) 60, 1236, 1532, 1669, 1837
BstNI CCWGG 1 cut(s) 1212
BstNSI RCATGY 1 cut(s) 453
BstSCI CCNGG 1 cut(s) 1210
BstV1I GCAGC 6 cut(s) 24, 66, 328, 1450, 1783, 2030
BstX2I RGATCY 5 cut(s) 6, 624, 1002, 1637, 1721
BstXI CCANNNNNNTGG 1 cut(s) 370
BstYI RGATCY 5 cut(s) 6, 624, 1002, 1637, 1721
BsuRI GGCC 7 cut(s) 185, 195, 215, 329, 472, 1831, 1876
BtgI CCRYGG 1 cut(s) 1112
BtgZI GCGATG 1 cut(s) 708
BtrI CACGTC 1 cut(s) 1576
BtsCI GGATG 8 cut(s) 252, 511, 1107, 1270, 1501, 1554, 1728, 1957
BtsI GCAGTG 1 cut(s) 1962
BtsIMutI CAGTG 4 cut(s) 111, 252, 397, 1962
BveI ACCTGC 1 cut(s) 1044
Cac8I GCNNGC 6 cut(s) 52, 217, 221, 1237, 1842, 2016
CciI TCATGA 1 cut(s) 346
CfoI GCGC 1 cut(s) 341
Cfr10I RCCGGY 1 cut(s) 215
Cfr13I GGNCC 5 cut(s) 213, 501, 1555, 1829, 1875
CseI GACGC 1 cut(s) 2010
Csp6I GTAC 5 cut(s) 423, 768, 931, 990, 1178
CspCI CAANNNNNGTGG 2 cut(s) 786, 821
CviAII CATG 7 cut(s) 146, 336, 347, 450, 1113, 1388, 2045
CviQI GTAC 5 cut(s) 423, 768, 931, 990, 1178
DdeI CTNAG 8 cut(s) 123, 579, 733, 1100, 1280, 1677, 1735, 1929
Eam1104I CTCTTC 5 cut(s) 29, 122, 203, 825, 992
EarI CTCTTC 5 cut(s) 29, 122, 203, 825, 992
EciI GGCGGA 1 cut(s) 890
Ecl136II GAGCTC 1 cut(s) 223
Eco130I CCWWGG 1 cut(s) 1112
Eco24I GRGCYC 1 cut(s) 225
Eco47I GGWCC 2 cut(s) 501, 1555
Eco53kI GAGCTC 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 311
EcoICRI GAGCTC 1 cut(s) 223
EcoRI GAATTC 2 cut(s) 1045, 1309
EcoRII CCWGG 1 cut(s) 1210
EcoT14I CCWWGG 1 cut(s) 1112
EcoT38I GRGCYC 1 cut(s) 225
ErhI CCWWGG 1 cut(s) 1112
Esp3I CGTCTC 1 cut(s) 2077
FaeI CATG 7 cut(s) 149, 339, 350, 453, 1116, 1391, 2048
FalI AAGNNNNNCTT 2 cut(s) 1783, 1815
FaqI GGGAC 4 cut(s) 644, 677, 1541, 1783
FatI CATG 7 cut(s) 145, 335, 346, 449, 1112, 1387, 2044
FauI CCCGC 4 cut(s) 660, 1411, 1675, 1760
FbaI TGATCA 1 cut(s) 73
Fnu4HI GCNGC 8 cut(s) 38, 55, 193, 342, 710, 1439, 1797, 2019
FokI GGATG 8 cut(s) 239, 498, 1094, 1277, 1508, 1561, 1715, 1964
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 8 cut(s) 38, 55, 193, 342, 710, 1439, 1797, 2019
FspBI CTAG 1 cut(s) 1457
GlaI GCGC 1 cut(s) 340
GluI GCNGC 8 cut(s) 38, 55, 193, 342, 710, 1439, 1797, 2019
HaeIII GGCC 7 cut(s) 185, 195, 215, 329, 472, 1831, 1876
HapII CCGG 1 cut(s) 216
HgaI GACGC 1 cut(s) 2010
HhaI GCGC 1 cut(s) 341
Hin1II CATG 7 cut(s) 149, 339, 350, 453, 1116, 1391, 2048
Hin6I GCGC 1 cut(s) 339
HinP1I GCGC 1 cut(s) 339
HincII GTYRAC 1 cut(s) 1207
HindII GTYRAC 1 cut(s) 1207
HinfI GANTC 4 cut(s) 359, 977, 1906, 1925
HpaII CCGG 1 cut(s) 216
HphI GGTGA 7 cut(s) 432, 850, 1031, 1228, 1508, 1511, 1659
Hpy166II GTNNAC 3 cut(s) 739, 1201, 1207
Hpy188I TCNGA 9 cut(s) 47, 758, 871, 987, 1165, 1253, 1476, 1531, 1726
Hpy188III TCNNGA 3 cut(s) 202, 347, 1314
Hpy8I GTNNAC 3 cut(s) 739, 1201, 1207
Hpy99I CGWCG 1 cut(s) 762
HpyAV CCTTC 6 cut(s) 286, 1361, 1575, 1585, 2033, 2082
HpyCH4III ACNGT 4 cut(s) 85, 119, 392, 743
HpyCH4IV ACGT 4 cut(s) 760, 1203, 1575, 1772
HpyCH4V TGCA 7 cut(s) 111, 180, 308, 323, 453, 1438, 1955
HpyF10VI GCNNNNNNNGC 5 cut(s) 60, 1236, 1532, 1669, 1837
HpyF3I CTNAG 8 cut(s) 123, 579, 733, 1100, 1280, 1677, 1735, 1929
HpySE526I ACGT 4 cut(s) 760, 1203, 1575, 1772
Hsp92II CATG 7 cut(s) 149, 339, 350, 453, 1116, 1391, 2048
HspAI GCGC 1 cut(s) 339
KroI GCCGGC 1 cut(s) 215
KroNI GCCGGC 1 cut(s) 217
Ksp22I TGATCA 1 cut(s) 73
LguI GCTCTTC 1 cut(s) 122
LmnI GCTCC 4 cut(s) 47, 318, 717, 1939
Lsp1109I GCAGC 6 cut(s) 24, 66, 328, 1450, 1783, 2030
LweI GCATC 4 cut(s) 1069, 1116, 1535, 1942
MaeI CTAG 1 cut(s) 1457
MaeII ACGT 4 cut(s) 760, 1203, 1575, 1772
MaeIII GTNAC 3 cut(s) 166, 978, 1712
MbiI CCGCTC 1 cut(s) 712
MflI RGATCY 5 cut(s) 6, 624, 1002, 1637, 1721
MhlI GDGCHC 1 cut(s) 225
MlyI GAGTC 2 cut(s) 986, 1919
MmeI TCCRAC 5 cut(s) 781, 1242, 1668, 1749, 1954
MroNI GCCGGC 1 cut(s) 215
MroXI GAANNNNTTC 1 cut(s) 965
MseI TTAA 8 cut(s) 135, 480, 885, 894, 1014, 1247, 1790, 1824
MslI CAYNNNNRTG 2 cut(s) 368, 448
MspI CCGG 1 cut(s) 216
MspR9I CCNGG 1 cut(s) 1212
Mva1269I GAATGC 2 cut(s) 113, 1663
MvaI CCWGG 1 cut(s) 1212
MwoI GCNNNNNNNGC 5 cut(s) 60, 1236, 1532, 1669, 1837
NaeI GCCGGC 1 cut(s) 217
NcoI CCATGG 1 cut(s) 1112
NgoMIV GCCGGC 1 cut(s) 215
NlaIII CATG 7 cut(s) 149, 339, 350, 453, 1116, 1391, 2048
NlaIV GGNNCC 3 cut(s) 214, 1557, 1639
NmeAIII GCCGAG 1 cut(s) 1124
NmuCI GTSAC 2 cut(s) 978, 1712
NspI RCATGY 1 cut(s) 453
NspV TTCGAA 1 cut(s) 959
OliI CACNNNNGTG 1 cut(s) 368
PagI TCATGA 1 cut(s) 346
PasI CCCWGGG 1 cut(s) 1211
PciSI GCTCTTC 1 cut(s) 122
PcsI WCGNNNNNNNCGW 1 cut(s) 2083
PctI GAATGC 2 cut(s) 113, 1663
PdiI GCCGGC 1 cut(s) 217
PdmI GAANNNNTTC 1 cut(s) 965
PfeI GAWTC 2 cut(s) 359, 1906
PkrI GCNGC 8 cut(s) 39, 56, 194, 343, 711, 1440, 1798, 2020
Ple19I CGATCG 1 cut(s) 174
PleI GAGTC 2 cut(s) 985, 1919
PpsI GAGTC 2 cut(s) 985, 1919
PsiI TTATAA 1 cut(s) 1965
Psp124BI GAGCTC 1 cut(s) 225
Psp6I CCWGG 1 cut(s) 1210
PspGI CCWGG 1 cut(s) 1210
PspN4I GGNNCC 3 cut(s) 214, 1557, 1639
PspPI GGNCC 5 cut(s) 213, 501, 1555, 1829, 1875
PsrI GAACNNNNNNTAC 2 cut(s) 1340, 1372
PsuI RGATCY 5 cut(s) 6, 624, 1002, 1637, 1721
PvuI CGATCG 1 cut(s) 174
RsaI GTAC 5 cut(s) 424, 769, 932, 991, 1179
RsaNI GTAC 5 cut(s) 423, 768, 931, 990, 1178
RseI CAYNNNNRTG 2 cut(s) 368, 448
SacI GAGCTC 1 cut(s) 225
SapI GCTCTTC 1 cut(s) 122
SaqAI TTAA 8 cut(s) 135, 480, 885, 894, 1014, 1247, 1790, 1824
SatI GCNGC 8 cut(s) 38, 55, 193, 342, 710, 1439, 1797, 2019
Sau96I GGNCC 5 cut(s) 213, 501, 1555, 1829, 1875
ScaI AGTACT 2 cut(s) 932, 991
SchI GAGTC 2 cut(s) 986, 1919
ScrFI CCNGG 1 cut(s) 1212
SduI GDGCHC 1 cut(s) 225
SfaNI GCATC 4 cut(s) 1069, 1116, 1535, 1942
SfuI TTCGAA 1 cut(s) 959
SinI GGWCC 2 cut(s) 501, 1555
SmiMI CAYNNNNRTG 2 cut(s) 368, 448
SmlI CTYRAG 4 cut(s) 1304, 1520, 1717, 1889
SmoI CTYRAG 4 cut(s) 1304, 1520, 1717, 1889
SspI AATATT 3 cut(s) 534, 1630, 1696
SspMI CTAG 1 cut(s) 1457
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 1 cut(s) 1210
StyI CCWWGG 1 cut(s) 1112
TaaI ACNGT 4 cut(s) 85, 119, 392, 743
TaiI ACGT 4 cut(s) 763, 1206, 1578, 1775
TaqI TCGA 3 cut(s) 174, 201, 959
TatI WGTACW 2 cut(s) 930, 989
TauI GCSGC 2 cut(s) 195, 712
TfiI GAWTC 2 cut(s) 359, 1906
Tru1I TTAA 8 cut(s) 135, 480, 885, 894, 1014, 1247, 1790, 1824
Tru9I TTAA 8 cut(s) 135, 480, 885, 894, 1014, 1247, 1790, 1824
TscAI CASTG 4 cut(s) 118, 252, 397, 1962
TseFI GTSAC 2 cut(s) 978, 1712
TseI GCWGC 6 cut(s) 37, 54, 341, 1438, 1796, 2018
Tsp45I GTSAC 2 cut(s) 978, 1712
TspDTI ATGAA 6 cut(s) 17, 1010, 1172, 1604, 1688, 2052
TspRI CASTG 4 cut(s) 118, 252, 397, 1962
VpaK11BI GGWCC 2 cut(s) 501, 1555
XapI RAATTY 5 cut(s) 554, 1045, 1309, 1804, 1869
XceI RCATGY 1 cut(s) 453
XcmI CCANNNNNNNNNTGG 1 cut(s) 495
XmnI GAANNNNTTC 1 cut(s) 965
XspI CTAG 1 cut(s) 1457
ZrmI AGTACT 2 cut(s) 932, 991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.