Rroxscaffold_7G00215400

Proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010868
Physical Location & Seq
Forward (+)
65963104 .. 65965472
2369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00215400.1

Sequence Viewer

Length: 1419 bp
ATGAAGATCTCTCATCTGTGCCTCTTCTTGTCCTTGCTGCTTTTAGGAGCGAGCAGCTTTTGCTATGCTGATGATCATAAGACAGTTGAGGTAGTTGGGTTTGGAGAATGTACTGACTGTGCTGAGAAGAGCATTAAGACCAGTCATGCTTTTTCAGGTCTTGGTGTTACGATCGACTGCAAGGCCGAAAGCGGCCACTTCGAAAGGAGAGGGGCCGGCGAGCTCAATGAGGAGGGCAAGTTTTCAGTGTCCATCCCAAAGGAGATTGTGAAAGATGGAGGAGATGGATTGAAGGAGGAGTGCTATGCACAGCTCCACAATGCAAAGGCCACACCATGTGCTGCTCATGATGGACTTGAATCCACCAAAGTGGTATTCAAGTCTAAAAACAGTGATGGGAAACAAACCTTTGGAATTGTTGGTACTAAACTGAAGTTTTCACCATCTACATGCACTTCTGCTTTCTTTTGGCCTCCCTTTAAACACCCACTTCCCAAATGGACCAAACCACATCCTCTCTTTGGACACCCAATTTTCCCTTTCCCTCCAAAAAAATTCCCTCCATTCCCACCTAAGATTACTATTCCTCCATTCTATAAGAAGCCCTACCCTCCCAAGATCCCTTTTTACAAAAAGCCCTTCCCCCCACCCGTGCCAATTTACAAAAAGCCCCTCCCCCACCAGTCCCAATTTACGAAAAGCCCCTCCCCCCACCAGTCCCAATCTACGAGAAGCCACTTCCTCCCCCAGTCCCAATATACAAGAAGCCGCTCCCTCCACCAGTACCAATATACAAGAAGCCGCTCCCTCCCCCAGTCCCAATATACAAGAAGCCACTCCCTCCTCCAGTACCAATATACAAGAAGCCGCTCCCTCCCCCAGTCCCAATATACAAGAAGCCCCTCCCTCCACCAGTCCCAATTTACAAGCCTCCACCAGTGCCGGTTTACAAGAAACCAAACCCTCCACCAGTACCTATCTACAAGAAACCATGCCCTCCACCGGTTCCAGTCTACAAGAAACCACTCCCTCCACCAGTACCAGTTTACAAAAAGTCCCATCCCCCAAAAGCACCCAAAAAATGTGCCCTCCACCAGTTCCAATGTACAAGAAGCCACTCCCTCCACCAGTCCCTGTATACAAAAAGCCACTCCCTCCACCAGTCCCTGTGTACAAAAAGCCACTCCCACCACCAGTCCCTGTGTACAAAAAGCCACTACCACCACCAGTCCCTGTGTACAAAAAGCCACTCCCACCACCAGTTCCAGTGTACAAAAAGCCACTCCCACCACCTGTTCCAGTGTACACAAAACCGCTTCCACCGCCGGTTCCTGTGTACACAAAACCGCTCCCACCACCGGTCCCAGTATGCATGGGACTCTGGAATTCCCAGCAAATCGAATTAATTCCAGTCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

472

Amino Acids

52.48

Weight (kDa)

9.82

Isoelectric Point (pI)

62.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 31 - 116 4.8e-10 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010868)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21140 AT4G38770
fragaria_vesca FvH4_2g01970
malus_domestica MD05G1116400.v1.1 MD10G1118600.v1.1
prunus_persica Prupe.8G160900_v2.0.a1
pyrus_communis pycom05g11060 pycom10g10270
rosa_chinensis RchiOBHm_Chr6g0245161
rosa_laevigata RLG00000015320
rosa_multiflora Rmu_sc0001288.1_g000002 Rmu_sc0015012.1_g000001 Rmu_ssc0000087.1_g000001
rosa_roxburghii Rroxscaffold_7G00215400
rosa_rugosa Rorug05G0512400
rosa_samantha Rh6AG027500 Rh6BG023600 Rh6CG022000 Rh6DG022100
rosa_wichuraiana Rw6G002320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 4 cut(s) 771, 804, 870, 1349
AccI GTMKAC 2 cut(s) 1013, 1138
AciI CCGC 7 cut(s) 192, 769, 802, 868, 1314, 1323, 1347
AclWI GGATC 1 cut(s) 613
AcoI YGGCCR 1 cut(s) 193
AcsI RAATTY 2 cut(s) 554, 1385
AcuI CTGAAG 1 cut(s) 452
AdeI CACNNNGTG 1 cut(s) 338
AfiI CCNNNNNNNGG 3 cut(s) 521, 1002, 1358
AgeI ACCGGT 2 cut(s) 1002, 1358
AgsI TTSAA 3 cut(s) 292, 359, 379
AjuI GAANNNNNNNTTGG 2 cut(s) 359, 391
AleI CACNNNNGTG 1 cut(s) 368
AluBI AGCT 3 cut(s) 57, 223, 313
AluI AGCT 3 cut(s) 57, 223, 313
Alw21I GWGCWC 1 cut(s) 225
AlwI GGATC 1 cut(s) 613
AlwNI CAGNNNCTG 4 cut(s) 1134, 1167, 1200, 1233
AoxI GGCC 5 cut(s) 183, 193, 213, 327, 470
ApeKI GCWGC 3 cut(s) 37, 54, 341
ApoI RAATTY 2 cut(s) 554, 1385
AseI ATTAAT 1 cut(s) 1404
AsiGI ACCGGT 2 cut(s) 1002, 1358
Asp700I GAANNNNTTC 1 cut(s) 1405
AspS9I GGNCC 3 cut(s) 213, 501, 1361
AsuHPI GGTGA 1 cut(s) 432
AsuII TTCGAA 1 cut(s) 201
AvaII GGWCC 2 cut(s) 501, 1361
BaeGI GKGCMC 1 cut(s) 1089
BanII GRGCYC 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 3 cut(s) 24, 66, 328
BccI CCATC 7 cut(s) 260, 269, 278, 344, 389, 451, 1067
BclI TGATCA 1 cut(s) 73
BglII AGATCT 1 cut(s) 6
BisI GCNGC 7 cut(s) 38, 55, 193, 342, 769, 802, 868
BlsI GCNGC 7 cut(s) 39, 56, 194, 343, 770, 803, 869
Bme18I GGWCC 2 cut(s) 501, 1361
BmgT120I GGNCC 3 cut(s) 213, 501, 1361
BmiI GGNNCC 4 cut(s) 214, 1007, 1330, 1363
BmrI ACTGGG 4 cut(s) 742, 808, 874, 1359
BmuI ACTGGG 4 cut(s) 742, 808, 874, 1359
BpmI CTGGAG 1 cut(s) 830
Bpu14I TTCGAA 1 cut(s) 201
BsaWI WCCGGW 2 cut(s) 1002, 1358
BsaXI ACNNNNNCTCC 4 cut(s) 224, 254, 571, 601
Bsc4I CCNNNNNNNGG 3 cut(s) 521, 1002, 1358
Bse118I RCCGGY 5 cut(s) 215, 942, 1002, 1325, 1358
BseGI GGATG 3 cut(s) 252, 511, 1059
BseLI CCNNNNNNNGG 3 cut(s) 521, 1002, 1358
BseMII CTCAG 1 cut(s) 114
BseRI GAGGAG 4 cut(s) 245, 294, 311, 833
BseSI GKGCMC 1 cut(s) 1089
BseXI GCAGC 3 cut(s) 24, 66, 328
BseYI CCCAGC 1 cut(s) 1390
Bsh1285I CGRYCG 1 cut(s) 174
BshFI GGCC 5 cut(s) 185, 195, 215, 329, 472
BshTI ACCGGT 2 cut(s) 1002, 1358
BsiEI CGRYCG 1 cut(s) 174
BsiHKAI GWGCWC 1 cut(s) 225
BsiSI CCGG 5 cut(s) 216, 943, 1003, 1326, 1359
BslI CCNNNNNNNGG 3 cut(s) 521, 1002, 1358
BsnI GGCC 5 cut(s) 185, 195, 215, 329, 472
Bsp119I TTCGAA 1 cut(s) 201
Bsp1286I GDGCHC 2 cut(s) 225, 1089
Bsp1407I TGTACA 7 cut(s) 1105, 1171, 1204, 1237, 1270, 1303, 1336
Bsp143I GATC 4 cut(s) 6, 73, 171, 618
BspACI CCGC 7 cut(s) 192, 769, 802, 868, 1314, 1323, 1347
BspANI GGCC 5 cut(s) 185, 195, 215, 329, 472
BspCNI CTCAG 1 cut(s) 115
BspHI TCATGA 1 cut(s) 346
BspLI GGNNCC 4 cut(s) 214, 1007, 1330, 1363
BspPI GGATC 1 cut(s) 613
BspQI GCTCTTC 1 cut(s) 122
BspT104I TTCGAA 1 cut(s) 201
BsrBI CCGCTC 4 cut(s) 771, 804, 870, 1349
BsrFI RCCGGY 5 cut(s) 215, 942, 1002, 1325, 1358
BsrGI TGTACA 7 cut(s) 1105, 1171, 1204, 1237, 1270, 1303, 1336
BssAI RCCGGY 5 cut(s) 215, 942, 1002, 1325, 1358
BssMI GATC 4 cut(s) 6, 73, 171, 618
BssNAI GTATAC 1 cut(s) 1139
Bst1107I GTATAC 1 cut(s) 1139
Bst4CI ACNGT 3 cut(s) 85, 119, 392
Bst6I CTCTTC 2 cut(s) 29, 122
BstAPI GCANNNNNTGC 1 cut(s) 60
BstAUI TGTACA 7 cut(s) 1105, 1171, 1204, 1237, 1270, 1303, 1336
BstBI TTCGAA 1 cut(s) 201
BstC8I GCNNGC 3 cut(s) 52, 217, 221
BstDEI CTNAG 2 cut(s) 123, 573
BstF5I GGATG 3 cut(s) 252, 511, 1059
BstKTI GATC 4 cut(s) 9, 76, 174, 621
BstMBI GATC 4 cut(s) 6, 73, 171, 618
BstMCI CGRYCG 1 cut(s) 174
BstMWI GCNNNNNNNGC 2 cut(s) 60, 1322
BstNSI RCATGY 1 cut(s) 453
BstSLI GKGCMC 1 cut(s) 1089
BstV1I GCAGC 3 cut(s) 24, 66, 328
BstX2I RGATCY 2 cut(s) 6, 618
BstXI CCANNNNNNTGG 1 cut(s) 370
BstYI RGATCY 2 cut(s) 6, 618
BstZ17I GTATAC 1 cut(s) 1139
BsuRI GGCC 5 cut(s) 185, 195, 215, 329, 472
BtsCI GGATG 3 cut(s) 252, 511, 1059
BtsIMutI CAGTG 5 cut(s) 252, 397, 944, 1273, 1306
Cac8I GCNNGC 3 cut(s) 52, 217, 221
CaiI CAGNNNCTG 4 cut(s) 1134, 1167, 1200, 1233
CciI TCATGA 1 cut(s) 346
Cfr10I RCCGGY 5 cut(s) 215, 942, 1002, 1325, 1358
Cfr13I GGNCC 3 cut(s) 213, 501, 1361
CspAI ACCGGT 2 cut(s) 1002, 1358
CviAII CATG 6 cut(s) 146, 336, 347, 450, 992, 1373
DdeI CTNAG 2 cut(s) 123, 573
DpnI GATC 4 cut(s) 8, 75, 173, 620
DpnII GATC 4 cut(s) 6, 73, 171, 618
DraI TTTAAA 1 cut(s) 481
DraIII CACNNNGTG 1 cut(s) 338
EaeI YGGCCR 1 cut(s) 193
Eam1104I CTCTTC 2 cut(s) 29, 122
EarI CTCTTC 2 cut(s) 29, 122
Ecl136II GAGCTC 1 cut(s) 223
Eco24I GRGCYC 1 cut(s) 225
Eco47I GGWCC 2 cut(s) 501, 1361
Eco53kI GAGCTC 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 452
EcoICRI GAGCTC 1 cut(s) 223
EcoRI GAATTC 1 cut(s) 1385
EcoT22I ATGCAT 1 cut(s) 1374
EcoT38I GRGCYC 1 cut(s) 225
FaeI CATG 6 cut(s) 149, 339, 350, 453, 995, 1376
FatI CATG 6 cut(s) 145, 335, 346, 449, 991, 1372
FbaI TGATCA 1 cut(s) 73
FblI GTMKAC 2 cut(s) 1013, 1138
Fnu4HI GCNGC 7 cut(s) 38, 55, 193, 342, 769, 802, 868
FokI GGATG 3 cut(s) 239, 498, 1046
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 7 cut(s) 38, 55, 193, 342, 769, 802, 868
GluI GCNGC 7 cut(s) 38, 55, 193, 342, 769, 802, 868
GsaI CCCAGC 1 cut(s) 1394
GsuI CTGGAG 1 cut(s) 830
HaeIII GGCC 5 cut(s) 185, 195, 215, 329, 472
HapII CCGG 5 cut(s) 216, 943, 1003, 1326, 1359
Hin1II CATG 6 cut(s) 149, 339, 350, 453, 995, 1376
HinfI GANTC 2 cut(s) 359, 1378
HpaII CCGG 5 cut(s) 216, 943, 1003, 1326, 1359
HphI GGTGA 1 cut(s) 432
Hpy188III TCNNGA 2 cut(s) 347, 1382
HpyAV CCTTC 2 cut(s) 286, 649
HpyCH4III ACNGT 3 cut(s) 85, 119, 392
HpyCH4V TGCA 5 cut(s) 180, 308, 323, 453, 1372
HpyF10VI GCNNNNNNNGC 2 cut(s) 60, 1322
HpyF3I CTNAG 2 cut(s) 123, 573
Hsp92II CATG 6 cut(s) 149, 339, 350, 453, 995, 1376
KroI GCCGGC 1 cut(s) 215
KroNI GCCGGC 1 cut(s) 217
Ksp22I TGATCA 1 cut(s) 73
Kzo9I GATC 4 cut(s) 6, 73, 171, 618
LguI GCTCTTC 1 cut(s) 122
LmnI GCTCC 6 cut(s) 47, 318, 776, 809, 875, 1354
Lsp1109I GCAGC 3 cut(s) 24, 66, 328
MaeIII GTNAC 1 cut(s) 166
MalI GATC 4 cut(s) 8, 75, 173, 620
MbiI CCGCTC 4 cut(s) 771, 804, 870, 1349
MboI GATC 4 cut(s) 6, 73, 171, 618
MboII GAAGA 3 cut(s) 16, 16, 139
MflI RGATCY 2 cut(s) 6, 618
MhlI GDGCHC 2 cut(s) 225, 1089
MluCI AATT 9 cut(s) 414, 531, 554, 657, 689, 920, 1385, 1401, 1405
MlyI GAGTC 1 cut(s) 1372
Mph1103I ATGCAT 1 cut(s) 1374
MroNI GCCGGC 1 cut(s) 215
MroXI GAANNNNTTC 1 cut(s) 1405
MseI TTAA 3 cut(s) 135, 480, 1404
MslI CAYNNNNRTG 2 cut(s) 368, 448
MspI CCGG 5 cut(s) 216, 943, 1003, 1326, 1359
MwoI GCNNNNNNNGC 2 cut(s) 60, 1322
NaeI GCCGGC 1 cut(s) 217
NdeII GATC 4 cut(s) 6, 73, 171, 618
NgoMIV GCCGGC 1 cut(s) 215
NlaIII CATG 6 cut(s) 149, 339, 350, 453, 995, 1376
NlaIV GGNNCC 4 cut(s) 214, 1007, 1330, 1363
NsiI ATGCAT 1 cut(s) 1374
NspI RCATGY 1 cut(s) 453
NspV TTCGAA 1 cut(s) 201
OliI CACNNNNGTG 1 cut(s) 368
PagI TCATGA 1 cut(s) 346
PciSI GCTCTTC 1 cut(s) 122
PdiI GCCGGC 1 cut(s) 217
PdmI GAANNNNTTC 1 cut(s) 1405
PfeI GAWTC 1 cut(s) 359
PinAI ACCGGT 2 cut(s) 1002, 1358
PkrI GCNGC 7 cut(s) 39, 56, 194, 343, 770, 803, 869
Ple19I CGATCG 1 cut(s) 174
PleI GAGTC 1 cut(s) 1372
PpsI GAGTC 1 cut(s) 1372
PshBI ATTAAT 1 cut(s) 1404
Psp124BI GAGCTC 1 cut(s) 225
PspFI CCCAGC 1 cut(s) 1390
PspN4I GGNNCC 4 cut(s) 214, 1007, 1330, 1363
PspPI GGNCC 3 cut(s) 213, 501, 1361
PstNI CAGNNNCTG 4 cut(s) 1134, 1167, 1200, 1233
PsuI RGATCY 2 cut(s) 6, 618
PvuI CGATCG 1 cut(s) 174
RseI CAYNNNNRTG 2 cut(s) 368, 448
SacI GAGCTC 1 cut(s) 225
SapI GCTCTTC 1 cut(s) 122
SaqAI TTAA 3 cut(s) 135, 480, 1404
SatI GCNGC 7 cut(s) 38, 55, 193, 342, 769, 802, 868
Sau3AI GATC 4 cut(s) 6, 73, 171, 618
Sau96I GGNCC 3 cut(s) 213, 501, 1361
SchI GAGTC 1 cut(s) 1372
SduI GDGCHC 2 cut(s) 225, 1089
SetI ASST 9 cut(s) 59, 93, 160, 225, 315, 410, 574, 978, 1295
SfuI TTCGAA 1 cut(s) 201
SinI GGWCC 2 cut(s) 501, 1361
SmiMI CAYNNNNRTG 2 cut(s) 368, 448
Sse9I AATT 9 cut(s) 414, 531, 554, 657, 689, 920, 1385, 1401, 1405
SsiI CCGC 7 cut(s) 192, 769, 802, 868, 1314, 1323, 1347
SstI GAGCTC 1 cut(s) 225
TaaI ACNGT 3 cut(s) 85, 119, 392
TaqI TCGA 3 cut(s) 174, 201, 1399
TasI AATT 9 cut(s) 414, 531, 554, 657, 689, 920, 1385, 1401, 1405
TatI WGTACW 8 cut(s) 110, 1105, 1171, 1204, 1237, 1270, 1303, 1336
TauI GCSGC 4 cut(s) 195, 771, 804, 870
TfiI GAWTC 1 cut(s) 359
Tru1I TTAA 3 cut(s) 135, 480, 1404
Tru9I TTAA 3 cut(s) 135, 480, 1404
TscAI CASTG 5 cut(s) 252, 397, 944, 1273, 1306
TseI GCWGC 3 cut(s) 37, 54, 341
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 5 cut(s) 252, 397, 944, 1273, 1306
VpaK11BI GGWCC 2 cut(s) 501, 1361
VspI ATTAAT 1 cut(s) 1404
XapI RAATTY 2 cut(s) 554, 1385
XceI RCATGY 1 cut(s) 453
XcmI CCANNNNNNNNNTGG 1 cut(s) 495
XmiI GTMKAC 2 cut(s) 1013, 1138
XmnI GAANNNNTTC 1 cut(s) 1405
Zsp2I ATGCAT 1 cut(s) 1374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.