Rh6CG022000

Proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0010868
Physical Location & Seq
Reverse (-)
2019821 .. 2023394
3574 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG022000.1

Sequence Viewer

Length: 1458 bp
ATGAAGATCTCTCATCTGTGCCTCTTCTTGTCCTTGCTGCTTTTCGGAGCGAGCAGCTTTTGCAATGCTGATGATCATAAGACAGTTGAGGTAGTTGGGTTTGGAGAATGTACTGACTGTGCTGAGAAGAGCATTAAGACCAGTCATGCTTTTTCAGGTCTTGGTGTTACGATCGACTGCAAGGCCGAAAGCGGGCACTTCGAGAGGAGAGGGGCCGGCGAGCTCAATGAGGAGGGCAAGTTTTCAGTGTCCATCCCAAAGGAGATTGTTAAAGATGGAGGAGATGGATTGAAGGAGGAGTGCTATGCACAGCTCCACAATGCAAAGGCCACACCATGTGCTGCTCATGATGGACTTGAATCCACCAAAGTGGTATTCAAGTCTAAAAACAGTGATGGGAAACAAACCTTTGGAATTGTTGGTACTAAACTGAAGTTTTCATCATCTACTTGCACTTCTGCTTTCTTTTGGCCTCACTTTAAACACCCACTTCCCAAATGGACCAAACCACATCCTCTCTTTGGACACCCAATATTCCCTTTCCCTCCAAAAAAATTCCCTCCATTCCCACCGAAGATTACTATTCCTCCATTCTATAAGAAGCCCTGCCCTCCCAAGGTCCCTTTTTACGAAAAGCCCCTCCCCCCGCCAGTCCCAATTTACGAAAAGCCCCTCCCCCCACCAGTCCCAATCTACAAGAAGCCGCTCCCCCCACCAGTCCCAATATACAAGAAGCCGCTCCCCCCACCAGTCCCAATCTACAAGAAGCCGCTCCCTCCCCCAGTCCCAATATACAAGAAGCCGCTCCCTCCACCAGTCCCAATTTACAAGCCTCCACCAGTGCCGGTTTACAAGAAACCAAACCCTCCACCAGTACCTATCTACAAGAAACCATGCCCTCCACCAGTTCCAATCTACAAGAAACCACTCCCTCCACCAGTCCCAGTGTACAAGAAGCCACTCCCTCCACCAGTCCCTGTGTACAAGAAGCCACTCCCTCCACCAGTCCCTGTGTACAAGAAGCCACTCCCACCACCAGTTCCAGTGTACAAAAAGCCGCTCCCACCACCAGTTCCAGTGTACACAAAACCGCTTCCACCACCGGTTCCTGTGTACACAAAACCGCTCCCACCACCAGTCCCAGTGTACACAAAACCACTCCCACCTCCAATCCCATTTTACAAACCAAAGCCACACCCATGGTTCAAGCCAAAACCACACCCATTTTACAAACCACTCCCACCTCCAATCCCATTTTACAAACCAAAGCCACACCCATTTTACAAACCACTCCCACCTCCAATCCCATTTTACAAACCAAAGCCACACCCATGGTTCAAGCCAAAGCCACACCCATTTTTCAAGCACCCATTCTTGAAGCCTCTACCTCCATTCCCCAAGATTCCTCACCATCCTTTCTTTAAGAAACCACCACCACAAACTACTCCCAAGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

485

Amino Acids

54.44

Weight (kDa)

9.88

Isoelectric Point (pI)

59.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 31 - 116 5e-10 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010868)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21140 AT4G38770
fragaria_vesca FvH4_2g01970
malus_domestica MD05G1116400.v1.1 MD10G1118600.v1.1
prunus_persica Prupe.8G160900_v2.0.a1
pyrus_communis pycom05g11060 pycom10g10270
rosa_chinensis RchiOBHm_Chr6g0245161
rosa_laevigata RLG00000015320
rosa_multiflora Rmu_sc0001288.1_g000002 Rmu_sc0015012.1_g000001 Rmu_ssc0000087.1_g000001
rosa_roxburghii Rroxscaffold_7G00215400
rosa_rugosa Rorug05G0512400
rosa_samantha Rh6AG027500 Rh6BG023600 Rh6CG022000 Rh6DG022100
rosa_wichuraiana Rw6G002320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 6 cut(s) 706, 739, 772, 805, 1060, 1126
AciI CCGC 9 cut(s) 192, 647, 704, 737, 770, 803, 1058, 1091, 1124
AcsI RAATTY 1 cut(s) 554
AcuI CTGAAG 1 cut(s) 452
AdeI CACNNNGTG 1 cut(s) 338
AfiI CCNNNNNNNGG 3 cut(s) 192, 521, 616
AgeI ACCGGT 1 cut(s) 1102
AgsI TTSAA 7 cut(s) 292, 359, 379, 1207, 1339, 1363, 1378
AjuI GAANNNNNNNTTGG 2 cut(s) 359, 391
AleI CACNNNNGTG 1 cut(s) 368
AluBI AGCT 3 cut(s) 57, 223, 313
AluI AGCT 3 cut(s) 57, 223, 313
Alw21I GWGCWC 1 cut(s) 225
AlwNI CAGNNNCTG 2 cut(s) 977, 1010
AoxI GGCC 4 cut(s) 183, 213, 327, 470
ApeKI GCWGC 3 cut(s) 37, 54, 341
ApoI RAATTY 1 cut(s) 554
AsiGI ACCGGT 1 cut(s) 1102
AspS9I GGNCC 3 cut(s) 213, 501, 619
AsuHPI GGTGA 1 cut(s) 1400
AvaII GGWCC 2 cut(s) 501, 619
BaeGI GKGCMC 1 cut(s) 198
BanII GRGCYC 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 3 cut(s) 24, 66, 328
BccI CCATC 6 cut(s) 260, 269, 278, 344, 389, 1419
BclI TGATCA 1 cut(s) 73
BglII AGATCT 1 cut(s) 6
BisI GCNGC 8 cut(s) 38, 55, 342, 704, 737, 770, 803, 1058
BlsI GCNGC 8 cut(s) 39, 56, 343, 705, 738, 771, 804, 1059
Bme18I GGWCC 2 cut(s) 501, 619
BmgT120I GGNCC 3 cut(s) 213, 501, 619
BmiI GGNNCC 3 cut(s) 214, 621, 1107
BmrI ACTGGG 3 cut(s) 776, 938, 1136
BmuI ACTGGG 3 cut(s) 776, 938, 1136
BsaJI CCNNGG 4 cut(s) 615, 1199, 1331, 1449
BsaWI WCCGGW 1 cut(s) 1102
BsaXI ACNNNNNCTCC 4 cut(s) 224, 254, 571, 601
Bsc4I CCNNNNNNNGG 3 cut(s) 192, 521, 616
Bse118I RCCGGY 3 cut(s) 215, 844, 1102
Bse3DI GCAATG 1 cut(s) 70
BseDI CCNNGG 4 cut(s) 615, 1199, 1331, 1449
BseGI GGATG 3 cut(s) 252, 511, 1411
BseLI CCNNNNNNNGG 3 cut(s) 192, 521, 616
BseMI GCAATG 1 cut(s) 70
BseMII CTCAG 1 cut(s) 114
BseRI GAGGAG 4 cut(s) 220, 245, 294, 311
BseSI GKGCMC 1 cut(s) 198
BseXI GCAGC 3 cut(s) 24, 66, 328
Bsh1285I CGRYCG 1 cut(s) 174
BshFI GGCC 4 cut(s) 185, 215, 329, 472
BshTI ACCGGT 1 cut(s) 1102
BsiEI CGRYCG 1 cut(s) 174
BsiHKAI GWGCWC 1 cut(s) 225
BsiSI CCGG 3 cut(s) 216, 845, 1103
BslI CCNNNNNNNGG 3 cut(s) 192, 521, 616
BsnI GGCC 4 cut(s) 185, 215, 329, 472
Bsp1286I GDGCHC 2 cut(s) 198, 225
Bsp1407I TGTACA 7 cut(s) 948, 981, 1014, 1047, 1080, 1113, 1146
Bsp143I GATC 3 cut(s) 6, 73, 171
Bsp19I CCATGG 2 cut(s) 1199, 1331
BspACI CCGC 9 cut(s) 192, 647, 704, 737, 770, 803, 1058, 1091, 1124
BspANI GGCC 4 cut(s) 185, 215, 329, 472
BspCNI CTCAG 1 cut(s) 115
BspHI TCATGA 1 cut(s) 346
BspLI GGNNCC 3 cut(s) 214, 621, 1107
BspQI GCTCTTC 1 cut(s) 122
BsrBI CCGCTC 6 cut(s) 706, 739, 772, 805, 1060, 1126
BsrDI GCAATG 1 cut(s) 70
BsrFI RCCGGY 3 cut(s) 215, 844, 1102
BsrGI TGTACA 7 cut(s) 948, 981, 1014, 1047, 1080, 1113, 1146
BssAI RCCGGY 3 cut(s) 215, 844, 1102
BssECI CCNNGG 4 cut(s) 615, 1199, 1331, 1449
BssMI GATC 3 cut(s) 6, 73, 171
BssT1I CCWWGG 4 cut(s) 615, 1199, 1331, 1449
Bst4CI ACNGT 3 cut(s) 85, 119, 392
Bst6I CTCTTC 2 cut(s) 29, 122
BstAPI GCANNNNNTGC 1 cut(s) 60
BstAUI TGTACA 7 cut(s) 948, 981, 1014, 1047, 1080, 1113, 1146
BstC8I GCNNGC 4 cut(s) 52, 194, 217, 221
BstDEI CTNAG 1 cut(s) 123
BstDSI CCRYGG 2 cut(s) 1199, 1331
BstF5I GGATG 3 cut(s) 252, 511, 1411
BstKTI GATC 3 cut(s) 9, 76, 174
BstMBI GATC 3 cut(s) 6, 73, 171
BstMCI CGRYCG 1 cut(s) 174
BstMWI GCNNNNNNNGC 1 cut(s) 60
BstSLI GKGCMC 1 cut(s) 198
BstV1I GCAGC 3 cut(s) 24, 66, 328
BstX2I RGATCY 1 cut(s) 6
BstXI CCANNNNNNTGG 3 cut(s) 370, 1200, 1332
BstYI RGATCY 1 cut(s) 6
BsuRI GGCC 4 cut(s) 185, 215, 329, 472
BtgI CCRYGG 2 cut(s) 1199, 1331
BtsCI GGATG 3 cut(s) 252, 511, 1411
BtsIMutI CAGTG 7 cut(s) 252, 397, 846, 951, 1050, 1083, 1149
Cac8I GCNNGC 4 cut(s) 52, 194, 217, 221
CaiI CAGNNNCTG 2 cut(s) 977, 1010
CciI TCATGA 1 cut(s) 346
Cfr10I RCCGGY 3 cut(s) 215, 844, 1102
Cfr13I GGNCC 3 cut(s) 213, 501, 619
CspAI ACCGGT 1 cut(s) 1102
CviAII CATG 6 cut(s) 146, 336, 347, 894, 1200, 1332
DdeI CTNAG 1 cut(s) 123
DpnI GATC 3 cut(s) 8, 75, 173
DpnII GATC 3 cut(s) 6, 73, 171
DraI TTTAAA 1 cut(s) 481
DraIII CACNNNGTG 1 cut(s) 338
Eam1104I CTCTTC 2 cut(s) 29, 122
EarI CTCTTC 2 cut(s) 29, 122
Ecl136II GAGCTC 1 cut(s) 223
Eco130I CCWWGG 4 cut(s) 615, 1199, 1331, 1449
Eco24I GRGCYC 1 cut(s) 225
Eco47I GGWCC 2 cut(s) 501, 619
Eco53kI GAGCTC 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 452
EcoICRI GAGCTC 1 cut(s) 223
EcoO109I RGGNCCY 1 cut(s) 619
EcoT14I CCWWGG 4 cut(s) 615, 1199, 1331, 1449
EcoT38I GRGCYC 1 cut(s) 225
ErhI CCWWGG 4 cut(s) 615, 1199, 1331, 1449
FaeI CATG 6 cut(s) 149, 339, 350, 897, 1203, 1335
FatI CATG 6 cut(s) 145, 335, 346, 893, 1199, 1331
FauI CCCGC 2 cut(s) 185, 654
FbaI TGATCA 1 cut(s) 73
Fnu4HI GCNGC 8 cut(s) 38, 55, 342, 704, 737, 770, 803, 1058
FokI GGATG 3 cut(s) 239, 498, 1398
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 8 cut(s) 38, 55, 342, 704, 737, 770, 803, 1058
GluI GCNGC 8 cut(s) 38, 55, 342, 704, 737, 770, 803, 1058
HaeIII GGCC 4 cut(s) 185, 215, 329, 472
HapII CCGG 3 cut(s) 216, 845, 1103
Hin1II CATG 6 cut(s) 149, 339, 350, 897, 1203, 1335
HinfI GANTC 2 cut(s) 359, 1402
HpaII CCGG 3 cut(s) 216, 845, 1103
HphI GGTGA 1 cut(s) 1400
Hpy188I TCNGA 1 cut(s) 47
Hpy188III TCNNGA 3 cut(s) 202, 347, 1375
HpyAV CCTTC 1 cut(s) 286
HpyCH4III ACNGT 3 cut(s) 85, 119, 392
HpyCH4V TGCA 5 cut(s) 63, 180, 308, 323, 453
HpyF10VI GCNNNNNNNGC 1 cut(s) 60
HpyF3I CTNAG 1 cut(s) 123
Hsp92II CATG 6 cut(s) 149, 339, 350, 897, 1203, 1335
KroI GCCGGC 1 cut(s) 215
KroNI GCCGGC 1 cut(s) 217
Ksp22I TGATCA 1 cut(s) 73
Kzo9I GATC 3 cut(s) 6, 73, 171
LguI GCTCTTC 1 cut(s) 122
LmnI GCTCC 8 cut(s) 47, 318, 711, 744, 777, 810, 1065, 1131
Lsp1109I GCAGC 3 cut(s) 24, 66, 328
MaeIII GTNAC 1 cut(s) 166
MalI GATC 3 cut(s) 8, 75, 173
MbiI CCGCTC 6 cut(s) 706, 739, 772, 805, 1060, 1126
MboI GATC 3 cut(s) 6, 73, 171
MboII GAAGA 4 cut(s) 16, 16, 139, 586
MflI RGATCY 1 cut(s) 6
MhlI GDGCHC 2 cut(s) 198, 225
MluCI AATT 4 cut(s) 414, 554, 657, 822
MroNI GCCGGC 1 cut(s) 215
MseI TTAA 5 cut(s) 135, 270, 480, 1422, 1456
MslI CAYNNNNRTG 3 cut(s) 368, 1198, 1330
MspI CCGG 3 cut(s) 216, 845, 1103
MwoI GCNNNNNNNGC 1 cut(s) 60
NaeI GCCGGC 1 cut(s) 217
NcoI CCATGG 2 cut(s) 1199, 1331
NdeII GATC 3 cut(s) 6, 73, 171
NgoMIV GCCGGC 1 cut(s) 215
NlaIII CATG 6 cut(s) 149, 339, 350, 897, 1203, 1335
NlaIV GGNNCC 3 cut(s) 214, 621, 1107
OliI CACNNNNGTG 1 cut(s) 368
PagI TCATGA 1 cut(s) 346
PciSI GCTCTTC 1 cut(s) 122
PdiI GCCGGC 1 cut(s) 217
PfeI GAWTC 2 cut(s) 359, 1402
PinAI ACCGGT 1 cut(s) 1102
PkrI GCNGC 8 cut(s) 39, 56, 343, 705, 738, 771, 804, 1059
Ple19I CGATCG 1 cut(s) 174
PpuMI RGGWCCY 1 cut(s) 619
Psp124BI GAGCTC 1 cut(s) 225
Psp5II RGGWCCY 1 cut(s) 619
PspN4I GGNNCC 3 cut(s) 214, 621, 1107
PspPI GGNCC 3 cut(s) 213, 501, 619
PspPPI RGGWCCY 1 cut(s) 619
PstNI CAGNNNCTG 2 cut(s) 977, 1010
PsuI RGATCY 1 cut(s) 6
PvuI CGATCG 1 cut(s) 174
RseI CAYNNNNRTG 3 cut(s) 368, 1198, 1330
SacI GAGCTC 1 cut(s) 225
SapI GCTCTTC 1 cut(s) 122
SaqAI TTAA 5 cut(s) 135, 270, 480, 1422, 1456
SatI GCNGC 8 cut(s) 38, 55, 342, 704, 737, 770, 803, 1058
Sau3AI GATC 3 cut(s) 6, 73, 171
Sau96I GGNCC 3 cut(s) 213, 501, 619
SduI GDGCHC 2 cut(s) 198, 225
SinI GGWCC 2 cut(s) 501, 619
SmiMI CAYNNNNRTG 3 cut(s) 368, 1198, 1330
Sse9I AATT 4 cut(s) 414, 554, 657, 822
SsiI CCGC 9 cut(s) 192, 647, 704, 737, 770, 803, 1058, 1091, 1124
SspI AATATT 1 cut(s) 534
SstI GAGCTC 1 cut(s) 225
StyI CCWWGG 4 cut(s) 615, 1199, 1331, 1449
TaaI ACNGT 3 cut(s) 85, 119, 392
TaqI TCGA 2 cut(s) 174, 201
TasI AATT 4 cut(s) 414, 554, 657, 822
TatI WGTACW 8 cut(s) 110, 948, 981, 1014, 1047, 1080, 1113, 1146
TauI GCSGC 5 cut(s) 706, 739, 772, 805, 1060
TfiI GAWTC 2 cut(s) 359, 1402
Tru1I TTAA 5 cut(s) 135, 270, 480, 1422, 1456
Tru9I TTAA 5 cut(s) 135, 270, 480, 1422, 1456
TscAI CASTG 7 cut(s) 252, 397, 846, 951, 1050, 1083, 1149
TseI GCWGC 3 cut(s) 37, 54, 341
TspDTI ATGAA 2 cut(s) 17, 429
TspRI CASTG 7 cut(s) 252, 397, 846, 951, 1050, 1083, 1149
VpaK11BI GGWCC 2 cut(s) 501, 619
XapI RAATTY 1 cut(s) 554
XcmI CCANNNNNNNNNTGG 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.