Rh6AG027500

Proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0010868
Physical Location & Seq
Reverse (-)
3433378 .. 3435805
2428 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG027500.1

Sequence Viewer

Length: 1902 bp
ATGAAGATCTCTCATCTGTGCCTCTTCTTGTCCTTGCTGCTTTTCGGAGCGAGCAGCTTTTGCTATGCTGATGATCATAAGACAGTTGAGGTAGTCGGGTTTGGAGAATGTACTGACTGTGCTGAGAAGAGCATTAAGACCAGTCATGCTTTTTCAGGTCTTGGTGTTACGATCGACTGCAAGGCCGAAAGCGGCCACTTCGAGAGGAGAGGGGCCGGCGAGCTCAATGAGGAGGGCAAGTTTTCAGTGTCCATCCCAAAGGAGATTGTGAAAGATGGAGGAGATGGACTGAAGGAGGAGTGCTATGCACAGCTCCACAATGCAAAGGCCACACCATGTGCTGCTCATGATGGACTTGAATCCACCAAAGTGGTATTCAAGTCTAAAAACAGTGATGGGAAACAAACCTTTGGAATTGTTGGTACTAAACTGAAGTTTTCACCATCTACATGCACTTCTGCTTTCTTTTGGCCTCCCTTTAAACACCCACTTCCCAAATGGACCAAACCACATCCTCTCTTTGGACACCCAATATTCTCTTTCCCTCCAAAAAAATTCCCTCCATTCCCACCTAAGATTACTATTCCTCCATTCTATAAGAAGCCCTGCCCTCCCAAGATCCCTTTTTACGAAAAGCCCCTCCCCCCGCCAGTCCCAATTTACGAAAAGCCCCTCCCCCCACCAGTCCCAATCTACGAAAAGCCCCTCCCCCCACCAGTCCCAATCTACAAGAAGCCCCTCCCCCCACCAGTCCCAATCTACAAGAAGCCCCTCCCTCCACCAGTCCCAATATACAAGAAGCCGCTCCCTCCCCCAGTCCCAATATACAAGAAGCCCCTCCCTCCACCAGTCCCAATCTACAAGAAGCCGCTCCCTCCACCAGTCCCAATATACAAGAAGCCGCTCCCTCCCCCAGTCCCAATATACAAGAAGCCCCTCCCTCCACCAGTCCCAATCTACAAGAAGCCCCTCCCTCCCCCAGTCCCAATCTACAAGAAGCCCCTCCCTCCACCAGTCCCAATCTACAAGAAGCCGCTCCCTCCCCCAGTCCCAATATACAAGAAGCCCTTCCCTCCACCAGTCCCAATTTACAAGCCTCCACCAGTGCCGGTTTACAAGAAACCAAACCCTCCACCAGTACCTATCTACAAGAAACCATGCCCTCCACCAGTTCCAGTCTACAAGAAACCACTCCCTCCACCAGTCCCAGTGTACAAGAAGCCACTCCCTCCACCAGTCCCTGTGTACAAGAAACCACTCCCTCCACCAGTCCCTGTATACAAGAAGCCACTCCCTCCACCAGTCCCTGTGTACAAAAAGCCACTCCCACCACCAGTCCCTGTGTATAAAAAGCCACTCCCACCACCAGTTCCAGTTTACAAAAAGCCACTCCCACCACCAGTTCCAGTTTACAAAAAACCACTCCCACCACCTGTTCCAGTGTACACAAAACCGCTTCCACCGCCGGTTCCTGTGTACACAAAACCGCTCCCACCACCGGTCCCAGTGTACACAAAACCACTCCCACCTCCAATCCCATTTTACAAACCAAAGCCACACCCATTTTACAAACCAAAACCACTTCCACCTCCAATTCCATTTTACAAACCAAAGCCACACCCATGGTTCAAGCCAAAGCCACTTCCACCTCCAATTCCATTTTACAAACCAAAGCCACACCCATGGTTCAAGCCAAAGCCACACCCATTTTACAAACCAAAACCACTTCCACCTCCAATCCCATTTTACAAACCAAAGCCACACCCATGGTTCAAGCCAAAGCCACACCCATTTTTCAAGCACCCATTCTTGAAGCCTCTACCTCCATTCCCCAAGATTCCTCATCATCCTTTCTTTAAGAAACCACCACCATTCCCACTACAAACATCTCCCAAGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

633

Amino Acids

71.17

Weight (kDa)

9.99

Isoelectric Point (pI)

67.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 31 - 116 7.6e-10 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010868)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21140 AT4G38770
fragaria_vesca FvH4_2g01970
malus_domestica MD05G1116400.v1.1 MD10G1118600.v1.1
prunus_persica Prupe.8G160900_v2.0.a1
pyrus_communis pycom05g11060 pycom10g10270
rosa_chinensis RchiOBHm_Chr6g0245161
rosa_laevigata RLG00000015320
rosa_multiflora Rmu_sc0001288.1_g000002 Rmu_sc0015012.1_g000001 Rmu_ssc0000087.1_g000001
rosa_roxburghii Rroxscaffold_7G00215400
rosa_rugosa Rorug05G0512400
rosa_samantha Rh6AG027500 Rh6BG023600 Rh6CG022000 Rh6DG022100
rosa_wichuraiana Rw6G002320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 5 cut(s) 805, 871, 904, 1036, 1489
AccI GTMKAC 2 cut(s) 1179, 1278
AciI CCGC 9 cut(s) 192, 647, 803, 869, 902, 1034, 1454, 1463, 1487
AclWI GGATC 1 cut(s) 613
AcoI YGGCCR 1 cut(s) 193
AcsI RAATTY 1 cut(s) 554
AcuI CTGAAG 2 cut(s) 311, 452
AdeI CACNNNGTG 1 cut(s) 338
AfaI GTAC 9 cut(s) 112, 424, 1140, 1214, 1247, 1313, 1445, 1478, 1511
AfiI CCNNNNNNNGG 2 cut(s) 521, 1498
AgeI ACCGGT 1 cut(s) 1498
AgsI TTSAA 7 cut(s) 359, 379, 1630, 1690, 1774, 1798, 1813
AjuI GAANNNNNNNTTGG 2 cut(s) 359, 391
AleI CACNNNNGTG 1 cut(s) 368
AluBI AGCT 3 cut(s) 57, 223, 313
AluI AGCT 3 cut(s) 57, 223, 313
Alw21I GWGCWC 1 cut(s) 225
AlwI GGATC 1 cut(s) 613
AlwNI CAGNNNCTG 4 cut(s) 1241, 1274, 1307, 1340
AoxI GGCC 5 cut(s) 183, 193, 213, 327, 470
ApeKI GCWGC 3 cut(s) 37, 54, 341
ApoI RAATTY 1 cut(s) 554
AsiGI ACCGGT 1 cut(s) 1498
Asp700I GAANNNNTTC 1 cut(s) 1067
AspS9I GGNCC 3 cut(s) 213, 501, 1501
AsuHPI GGTGA 1 cut(s) 432
AvaII GGWCC 2 cut(s) 501, 1501
BanII GRGCYC 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 3 cut(s) 24, 66, 328
BccI CCATC 6 cut(s) 260, 269, 278, 344, 389, 451
BclI TGATCA 1 cut(s) 73
BglII AGATCT 1 cut(s) 6
BisI GCNGC 8 cut(s) 38, 55, 193, 342, 803, 869, 902, 1034
BlsI GCNGC 8 cut(s) 39, 56, 194, 343, 804, 870, 903, 1035
Bme18I GGWCC 2 cut(s) 501, 1501
BmgT120I GGNCC 3 cut(s) 213, 501, 1501
BmiI GGNNCC 3 cut(s) 214, 1470, 1503
BmrI ACTGGG 6 cut(s) 809, 908, 974, 1040, 1202, 1499
BmuI ACTGGG 6 cut(s) 809, 908, 974, 1040, 1202, 1499
BsaJI CCNNGG 4 cut(s) 1622, 1682, 1766, 1893
BsaWI WCCGGW 1 cut(s) 1498
BsaXI ACNNNNNCTCC 4 cut(s) 224, 254, 571, 601
Bsc4I CCNNNNNNNGG 2 cut(s) 521, 1498
Bse118I RCCGGY 4 cut(s) 215, 1108, 1465, 1498
BseDI CCNNGG 4 cut(s) 1622, 1682, 1766, 1893
BseGI GGATG 3 cut(s) 252, 511, 1846
BseLI CCNNNNNNNGG 2 cut(s) 521, 1498
BseMII CTCAG 1 cut(s) 114
BseRI GAGGAG 4 cut(s) 220, 245, 294, 311
BseXI GCAGC 3 cut(s) 24, 66, 328
Bsh1285I CGRYCG 1 cut(s) 174
BshFI GGCC 5 cut(s) 185, 195, 215, 329, 472
BshTI ACCGGT 1 cut(s) 1498
BsiEI CGRYCG 1 cut(s) 174
BsiHKAI GWGCWC 1 cut(s) 225
BsiSI CCGG 4 cut(s) 216, 1109, 1466, 1499
BslI CCNNNNNNNGG 2 cut(s) 521, 1498
BsnI GGCC 5 cut(s) 185, 195, 215, 329, 472
Bsp1286I GDGCHC 1 cut(s) 225
Bsp1407I TGTACA 6 cut(s) 1212, 1245, 1311, 1443, 1476, 1509
Bsp143I GATC 4 cut(s) 6, 73, 171, 618
Bsp19I CCATGG 3 cut(s) 1622, 1682, 1766
BspACI CCGC 9 cut(s) 192, 647, 803, 869, 902, 1034, 1454, 1463, 1487
BspANI GGCC 5 cut(s) 185, 195, 215, 329, 472
BspCNI CTCAG 1 cut(s) 115
BspHI TCATGA 1 cut(s) 346
BspLI GGNNCC 3 cut(s) 214, 1470, 1503
BspPI GGATC 1 cut(s) 613
BspQI GCTCTTC 1 cut(s) 122
BsrBI CCGCTC 5 cut(s) 805, 871, 904, 1036, 1489
BsrFI RCCGGY 4 cut(s) 215, 1108, 1465, 1498
BsrGI TGTACA 6 cut(s) 1212, 1245, 1311, 1443, 1476, 1509
BssAI RCCGGY 4 cut(s) 215, 1108, 1465, 1498
BssECI CCNNGG 4 cut(s) 1622, 1682, 1766, 1893
BssMI GATC 4 cut(s) 6, 73, 171, 618
BssNAI GTATAC 1 cut(s) 1279
BssT1I CCWWGG 4 cut(s) 1622, 1682, 1766, 1893
Bst1107I GTATAC 1 cut(s) 1279
Bst4CI ACNGT 3 cut(s) 85, 119, 392
Bst6I CTCTTC 2 cut(s) 29, 122
BstAPI GCANNNNNTGC 1 cut(s) 60
BstAUI TGTACA 6 cut(s) 1212, 1245, 1311, 1443, 1476, 1509
BstC8I GCNNGC 3 cut(s) 52, 217, 221
BstDEI CTNAG 2 cut(s) 123, 573
BstDSI CCRYGG 3 cut(s) 1622, 1682, 1766
BstF5I GGATG 3 cut(s) 252, 511, 1846
BstKTI GATC 4 cut(s) 9, 76, 174, 621
BstMBI GATC 4 cut(s) 6, 73, 171, 618
BstMCI CGRYCG 1 cut(s) 174
BstMWI GCNNNNNNNGC 2 cut(s) 60, 1462
BstNSI RCATGY 1 cut(s) 453
BstV1I GCAGC 3 cut(s) 24, 66, 328
BstX2I RGATCY 2 cut(s) 6, 618
BstXI CCANNNNNNTGG 4 cut(s) 370, 1623, 1683, 1767
BstYI RGATCY 2 cut(s) 6, 618
BstZ17I GTATAC 1 cut(s) 1279
BsuRI GGCC 5 cut(s) 185, 195, 215, 329, 472
BtgI CCRYGG 3 cut(s) 1622, 1682, 1766
BtsCI GGATG 3 cut(s) 252, 511, 1846
BtsIMutI CAGTG 6 cut(s) 252, 397, 1110, 1215, 1446, 1512
Cac8I GCNNGC 3 cut(s) 52, 217, 221
CaiI CAGNNNCTG 4 cut(s) 1241, 1274, 1307, 1340
CciI TCATGA 1 cut(s) 346
Cfr10I RCCGGY 4 cut(s) 215, 1108, 1465, 1498
Cfr13I GGNCC 3 cut(s) 213, 501, 1501
Csp6I GTAC 9 cut(s) 111, 423, 1139, 1213, 1246, 1312, 1444, 1477, 1510
CspAI ACCGGT 1 cut(s) 1498
CviAII CATG 8 cut(s) 146, 336, 347, 450, 1158, 1623, 1683, 1767
CviQI GTAC 9 cut(s) 111, 423, 1139, 1213, 1246, 1312, 1444, 1477, 1510
DdeI CTNAG 2 cut(s) 123, 573
DpnI GATC 4 cut(s) 8, 75, 173, 620
DpnII GATC 4 cut(s) 6, 73, 171, 618
DraI TTTAAA 1 cut(s) 481
DraIII CACNNNGTG 1 cut(s) 338
EaeI YGGCCR 1 cut(s) 193
Eam1104I CTCTTC 2 cut(s) 29, 122
EarI CTCTTC 2 cut(s) 29, 122
Ecl136II GAGCTC 1 cut(s) 223
Eco130I CCWWGG 4 cut(s) 1622, 1682, 1766, 1893
Eco24I GRGCYC 1 cut(s) 225
Eco47I GGWCC 2 cut(s) 501, 1501
Eco53kI GAGCTC 1 cut(s) 223
Eco57I CTGAAG 2 cut(s) 311, 452
EcoICRI GAGCTC 1 cut(s) 223
EcoT14I CCWWGG 4 cut(s) 1622, 1682, 1766, 1893
EcoT38I GRGCYC 1 cut(s) 225
ErhI CCWWGG 4 cut(s) 1622, 1682, 1766, 1893
FaeI CATG 8 cut(s) 149, 339, 350, 453, 1161, 1626, 1686, 1770
FalI AAGNNNNNCTT 2 cut(s) 1052, 1084
FatI CATG 8 cut(s) 145, 335, 346, 449, 1157, 1622, 1682, 1766
FauI CCCGC 1 cut(s) 654
FbaI TGATCA 1 cut(s) 73
FblI GTMKAC 2 cut(s) 1179, 1278
Fnu4HI GCNGC 8 cut(s) 38, 55, 193, 342, 803, 869, 902, 1034
FokI GGATG 3 cut(s) 239, 498, 1833
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 8 cut(s) 38, 55, 193, 342, 803, 869, 902, 1034
GluI GCNGC 8 cut(s) 38, 55, 193, 342, 803, 869, 902, 1034
HaeIII GGCC 5 cut(s) 185, 195, 215, 329, 472
HapII CCGG 4 cut(s) 216, 1109, 1466, 1499
Hin1II CATG 8 cut(s) 149, 339, 350, 453, 1161, 1626, 1686, 1770
HinfI GANTC 2 cut(s) 359, 1837
HpaII CCGG 4 cut(s) 216, 1109, 1466, 1499
HphI GGTGA 1 cut(s) 432
Hpy188I TCNGA 1 cut(s) 47
Hpy188III TCNNGA 3 cut(s) 202, 347, 1810
HpyAV CCTTC 2 cut(s) 286, 1078
HpyCH4III ACNGT 3 cut(s) 85, 119, 392
HpyCH4V TGCA 4 cut(s) 180, 308, 323, 453
HpyF10VI GCNNNNNNNGC 2 cut(s) 60, 1462
HpyF3I CTNAG 2 cut(s) 123, 573
Hsp92II CATG 8 cut(s) 149, 339, 350, 453, 1161, 1626, 1686, 1770
KroI GCCGGC 1 cut(s) 215
KroNI GCCGGC 1 cut(s) 217
Ksp22I TGATCA 1 cut(s) 73
Kzo9I GATC 4 cut(s) 6, 73, 171, 618
LguI GCTCTTC 1 cut(s) 122
LmnI GCTCC 7 cut(s) 47, 318, 810, 876, 909, 1041, 1494
Lsp1109I GCAGC 3 cut(s) 24, 66, 328
MaeIII GTNAC 1 cut(s) 166
MalI GATC 4 cut(s) 8, 75, 173, 620
MbiI CCGCTC 5 cut(s) 805, 871, 904, 1036, 1489
MboI GATC 4 cut(s) 6, 73, 171, 618
MboII GAAGA 3 cut(s) 16, 16, 139
MflI RGATCY 2 cut(s) 6, 618
MhlI GDGCHC 1 cut(s) 225
MluCI AATT 6 cut(s) 414, 554, 657, 1086, 1593, 1653
MroNI GCCGGC 1 cut(s) 215
MroXI GAANNNNTTC 1 cut(s) 1067
MseI TTAA 4 cut(s) 135, 480, 1857, 1900
MslI CAYNNNNRTG 5 cut(s) 368, 448, 1621, 1681, 1765
MspI CCGG 4 cut(s) 216, 1109, 1466, 1499
MwoI GCNNNNNNNGC 2 cut(s) 60, 1462
NaeI GCCGGC 1 cut(s) 217
NcoI CCATGG 3 cut(s) 1622, 1682, 1766
NdeII GATC 4 cut(s) 6, 73, 171, 618
NgoMIV GCCGGC 1 cut(s) 215
NlaIII CATG 8 cut(s) 149, 339, 350, 453, 1161, 1626, 1686, 1770
NlaIV GGNNCC 3 cut(s) 214, 1470, 1503
NspI RCATGY 1 cut(s) 453
OliI CACNNNNGTG 1 cut(s) 368
PagI TCATGA 1 cut(s) 346
PciSI GCTCTTC 1 cut(s) 122
PdiI GCCGGC 1 cut(s) 217
PdmI GAANNNNTTC 1 cut(s) 1067
PfeI GAWTC 2 cut(s) 359, 1837
PinAI ACCGGT 1 cut(s) 1498
PkrI GCNGC 8 cut(s) 39, 56, 194, 343, 804, 870, 903, 1035
Ple19I CGATCG 1 cut(s) 174
Psp124BI GAGCTC 1 cut(s) 225
PspN4I GGNNCC 3 cut(s) 214, 1470, 1503
PspPI GGNCC 3 cut(s) 213, 501, 1501
PstNI CAGNNNCTG 4 cut(s) 1241, 1274, 1307, 1340
PsuI RGATCY 2 cut(s) 6, 618
PvuI CGATCG 1 cut(s) 174
RsaI GTAC 9 cut(s) 112, 424, 1140, 1214, 1247, 1313, 1445, 1478, 1511
RsaNI GTAC 9 cut(s) 111, 423, 1139, 1213, 1246, 1312, 1444, 1477, 1510
RseI CAYNNNNRTG 5 cut(s) 368, 448, 1621, 1681, 1765
SacI GAGCTC 1 cut(s) 225
SapI GCTCTTC 1 cut(s) 122
SaqAI TTAA 4 cut(s) 135, 480, 1857, 1900
SatI GCNGC 8 cut(s) 38, 55, 193, 342, 803, 869, 902, 1034
Sau3AI GATC 4 cut(s) 6, 73, 171, 618
Sau96I GGNCC 3 cut(s) 213, 501, 1501
SduI GDGCHC 1 cut(s) 225
SinI GGWCC 2 cut(s) 501, 1501
SmiMI CAYNNNNRTG 5 cut(s) 368, 448, 1621, 1681, 1765
Sse9I AATT 6 cut(s) 414, 554, 657, 1086, 1593, 1653
SsiI CCGC 9 cut(s) 192, 647, 803, 869, 902, 1034, 1454, 1463, 1487
SspI AATATT 1 cut(s) 534
SstI GAGCTC 1 cut(s) 225
StyI CCWWGG 4 cut(s) 1622, 1682, 1766, 1893
TaaI ACNGT 3 cut(s) 85, 119, 392
TaqI TCGA 2 cut(s) 174, 201
TasI AATT 6 cut(s) 414, 554, 657, 1086, 1593, 1653
TatI WGTACW 7 cut(s) 110, 1212, 1245, 1311, 1443, 1476, 1509
TauI GCSGC 5 cut(s) 195, 805, 871, 904, 1036
TfiI GAWTC 2 cut(s) 359, 1837
Tru1I TTAA 4 cut(s) 135, 480, 1857, 1900
Tru9I TTAA 4 cut(s) 135, 480, 1857, 1900
TscAI CASTG 6 cut(s) 252, 397, 1110, 1215, 1446, 1512
TseI GCWGC 3 cut(s) 37, 54, 341
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 6 cut(s) 252, 397, 1110, 1215, 1446, 1512
VpaK11BI GGWCC 2 cut(s) 501, 1501
XapI RAATTY 1 cut(s) 554
XceI RCATGY 1 cut(s) 453
XcmI CCANNNNNNNNNTGG 1 cut(s) 495
XmiI GTMKAC 2 cut(s) 1179, 1278
XmnI GAANNNNTTC 1 cut(s) 1067
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.