RLG00000015320

Proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0010868
Physical Location & Seq
Forward (+)
64499110 .. 64500784
1675 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015320

Sequence Viewer

Length: 1575 bp
ATGAAGATCTCTCATCTGTGCCTCTTCTTGTCCTTGCTGCTTTTCGGAGCGACCAGCTTTTGCTATGCTGATGATCATAAGACAGTTGAGGTAGTTGGGTTTGGAGAATGTACTGACTGTGCTGAGAAGAGCATTAAGACCAGTCATGCTTTTTCAGGTCTTGGTGTTACGATTGACTGCAAGGCCGAAAGCGGGCACTTCGAGAGGAGAGGGGCCGGCGAGCTCGATGAGGAGGGCAAGTTTTCAGTTTCCATCCCAAAGGAGATTGTTAAAGATGGAGGAGATGGATTGAAGGAGGAGTGCTATGCACAGCTCCACAATGCAAAGGCCACACCATGTGCTGCTCATGATGGACTTGAATCCACCAAAGTGGTATTCAAGTCTAAAAACAGTGATGGGAAACAAACCTTTGGAATTGTTGGTACTAAACTGAAGTTTTCACCATCAACATGCACTTCTGCTTTCTTTTGGCCTCCCTTTAAACACCCACTTCCCAAATGGACCAAACCACATCCTCTCTTTGGACACCCAATTTTCCCTTTCCCTCCAAAAAAATTCCCTCCATTCCCACCTAAGATTACTATTCCTCCATTCTATAAGAAGCCCTGCCCTCCCAAGATCCCTTTTTACGAAAAGCCCCTCCCCCCACCAGTCCCAATCTACGAAAAGCCCCTCCCCCCACCAGTCCCAATCTACGAGAAGCCACTCCCTCCCCCAGTCCCAATATACAAGAAGCCACTCCCTCCACCAGTACCAGTATACAAGAAGCCGCTCCCTCCCCCAGTCCCAATATACAAGAAGCCCCTCCCTCCACCAGTCCCAGTTTACAAGCCTCCACCAGTGCCGGTTTACAAGAAACCAAACCCTCCACCAGTACCTATCTACAAGAAACCATGCCCTCCACCAGTTCCAGTCTACAAGAAACCACTCCCTCCACCAGTACCAGTTTACAAAAAGCCCCATCCCCCAAAAGCACCCAAAAAAGTGTGCCCTCCACCAGTCCCAGTGTACAAAAAGCCACTCCCTCCACCAGTCCCTGTGTACAAAAAGCCACTCCCACCACCAGTTCCAGTGTACAAAAAGCCACTCCCACCACCGGTTCCAGTGTACACAAAACCGCTTCCACCGCCGGTTCCTGTATACACAAAACCGCTTCCACCGCCGGTTCCTGTGTACACAAAACCGCTCCCACCGCCTGTCCCAGTGTACACAAAACCGCTCCCACCTCCAATCCCATTTTACAAACCAAAGCCACACCCATGGTTCAAGCCAAAGCCACACCCATTTTACAAACCAAAACCACTTCCACCTCCAATCCCATTTTACAAACCAAAACCACTTCCACCTCCAATCCCATTTTACAAACCAAAGCCACACCCATGGTTCAAGCCAAAGCCACACCCATTTTACAAACCAAAACCACTTCCACCTCCAATCCCATTTTACAAACCAAAGCCACACCCACTTTTCAAGCACCCATTCTTGAAGCCTCTACCCCCATTCCCCAAGATTCCCCATCATCCTTTCTTTAAGAAACCACCACCATTCCCACTACAAACATCTCCCAAGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

525

Amino Acids

58.75

Weight (kDa)

9.88

Isoelectric Point (pI)

62.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 31 - 116 2.2e-10 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010868)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21140 AT4G38770
fragaria_vesca FvH4_2g01970
malus_domestica MD05G1116400.v1.1 MD10G1118600.v1.1
prunus_persica Prupe.8G160900_v2.0.a1
pyrus_communis pycom05g11060 pycom10g10270
rosa_chinensis RchiOBHm_Chr6g0245161
rosa_laevigata RLG00000015320
rosa_multiflora Rmu_sc0001288.1_g000002 Rmu_sc0015012.1_g000001 Rmu_ssc0000087.1_g000001
rosa_roxburghii Rroxscaffold_7G00215400
rosa_rugosa Rorug05G0512400
rosa_samantha Rh6AG027500 Rh6BG023600 Rh6CG022000 Rh6DG022100
rosa_wichuraiana Rw6G002320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 3 cut(s) 772, 1186, 1219
AccI GTMKAC 3 cut(s) 759, 915, 1140
AciI CCGC 9 cut(s) 192, 770, 1118, 1127, 1151, 1160, 1184, 1193, 1217
AclWI GGATC 1 cut(s) 613
AcsI RAATTY 1 cut(s) 554
AcuI CTGAAG 1 cut(s) 452
AdeI CACNNNGTG 1 cut(s) 338
AfiI CCNNNNNNNGG 3 cut(s) 192, 521, 1096
AgeI ACCGGT 1 cut(s) 1096
AgsI TTSAA 7 cut(s) 292, 359, 379, 1267, 1387, 1471, 1486
AjuI GAANNNNNNNTTGG 2 cut(s) 359, 391
AleI CACNNNNGTG 1 cut(s) 368
AluBI AGCT 3 cut(s) 57, 223, 313
AluI AGCT 3 cut(s) 57, 223, 313
Alw21I GWGCWC 1 cut(s) 225
AlwI GGATC 1 cut(s) 613
AlwNI CAGNNNCTG 1 cut(s) 1037
AoxI GGCC 4 cut(s) 183, 213, 327, 470
ApeKI GCWGC 2 cut(s) 37, 341
ApoI RAATTY 1 cut(s) 554
AsiGI ACCGGT 1 cut(s) 1096
AspS9I GGNCC 2 cut(s) 213, 501
AsuHPI GGTGA 1 cut(s) 432
AvaII GGWCC 1 cut(s) 501
BaeGI GKGCMC 2 cut(s) 198, 992
BanII GRGCYC 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 2 cut(s) 24, 328
BccI CCATC 8 cut(s) 260, 269, 278, 344, 389, 451, 969, 1524
BclI TGATCA 1 cut(s) 73
BglII AGATCT 1 cut(s) 6
BisI GCNGC 3 cut(s) 38, 342, 770
BlsI GCNGC 3 cut(s) 39, 343, 771
Bme18I GGWCC 1 cut(s) 501
BmgT120I GGNCC 2 cut(s) 213, 501
BmiI GGNNCC 4 cut(s) 214, 1101, 1134, 1167
BmrI ACTGGG 5 cut(s) 710, 776, 815, 998, 1196
BmuI ACTGGG 5 cut(s) 710, 776, 815, 998, 1196
BsaJI CCNNGG 3 cut(s) 1259, 1379, 1566
BsaWI WCCGGW 1 cut(s) 1096
BsaXI ACNNNNNCTCC 4 cut(s) 224, 254, 571, 601
Bsc4I CCNNNNNNNGG 3 cut(s) 192, 521, 1096
Bse118I RCCGGY 5 cut(s) 215, 844, 1096, 1129, 1162
BseDI CCNNGG 3 cut(s) 1259, 1379, 1566
BseGI GGATG 4 cut(s) 252, 511, 961, 1519
BseLI CCNNNNNNNGG 3 cut(s) 192, 521, 1096
BseMII CTCAG 1 cut(s) 114
BseRI GAGGAG 4 cut(s) 220, 245, 294, 311
BseSI GKGCMC 2 cut(s) 198, 992
BseXI GCAGC 2 cut(s) 24, 328
BshFI GGCC 4 cut(s) 185, 215, 329, 472
BshTI ACCGGT 1 cut(s) 1096
BsiHKAI GWGCWC 1 cut(s) 225
BsiSI CCGG 5 cut(s) 216, 845, 1097, 1130, 1163
BslFI GGGAC 8 cut(s) 638, 671, 704, 770, 803, 986, 1019, 1184
BslI CCNNNNNNNGG 3 cut(s) 192, 521, 1096
BsmFI GGGAC 8 cut(s) 638, 671, 704, 770, 803, 986, 1019, 1184
BsnI GGCC 4 cut(s) 185, 215, 329, 472
Bsp1286I GDGCHC 3 cut(s) 198, 225, 992
Bsp1407I TGTACA 6 cut(s) 1008, 1041, 1074, 1107, 1173, 1206
Bsp143I GATC 3 cut(s) 6, 73, 618
Bsp19I CCATGG 2 cut(s) 1259, 1379
BspACI CCGC 9 cut(s) 192, 770, 1118, 1127, 1151, 1160, 1184, 1193, 1217
BspANI GGCC 4 cut(s) 185, 215, 329, 472
BspCNI CTCAG 1 cut(s) 115
BspHI TCATGA 1 cut(s) 346
BspLI GGNNCC 4 cut(s) 214, 1101, 1134, 1167
BspPI GGATC 1 cut(s) 613
BspQI GCTCTTC 1 cut(s) 122
BsrBI CCGCTC 3 cut(s) 772, 1186, 1219
BsrFI RCCGGY 5 cut(s) 215, 844, 1096, 1129, 1162
BsrGI TGTACA 6 cut(s) 1008, 1041, 1074, 1107, 1173, 1206
BssAI RCCGGY 5 cut(s) 215, 844, 1096, 1129, 1162
BssECI CCNNGG 3 cut(s) 1259, 1379, 1566
BssMI GATC 3 cut(s) 6, 73, 618
BssNAI GTATAC 2 cut(s) 760, 1141
BssT1I CCWWGG 3 cut(s) 1259, 1379, 1566
Bst1107I GTATAC 2 cut(s) 760, 1141
Bst4CI ACNGT 3 cut(s) 85, 119, 392
Bst6I CTCTTC 2 cut(s) 29, 122
BstAUI TGTACA 6 cut(s) 1008, 1041, 1074, 1107, 1173, 1206
BstC8I GCNNGC 3 cut(s) 194, 217, 221
BstDEI CTNAG 2 cut(s) 123, 573
BstDSI CCRYGG 2 cut(s) 1259, 1379
BstF5I GGATG 4 cut(s) 252, 511, 961, 1519
BstKTI GATC 3 cut(s) 9, 76, 621
BstMBI GATC 3 cut(s) 6, 73, 618
BstMWI GCNNNNNNNGC 3 cut(s) 1126, 1159, 1192
BstNSI RCATGY 1 cut(s) 453
BstSLI GKGCMC 2 cut(s) 198, 992
BstV1I GCAGC 2 cut(s) 24, 328
BstX2I RGATCY 2 cut(s) 6, 618
BstXI CCANNNNNNTGG 3 cut(s) 370, 1260, 1380
BstYI RGATCY 2 cut(s) 6, 618
BstZ17I GTATAC 2 cut(s) 760, 1141
BsuRI GGCC 4 cut(s) 185, 215, 329, 472
BtgI CCRYGG 2 cut(s) 1259, 1379
BtsCI GGATG 4 cut(s) 252, 511, 961, 1519
BtsIMutI CAGTG 6 cut(s) 397, 846, 1011, 1077, 1110, 1209
Cac8I GCNNGC 3 cut(s) 194, 217, 221
CaiI CAGNNNCTG 1 cut(s) 1037
CciI TCATGA 1 cut(s) 346
Cfr10I RCCGGY 5 cut(s) 215, 844, 1096, 1129, 1162
Cfr13I GGNCC 2 cut(s) 213, 501
CspAI ACCGGT 1 cut(s) 1096
CviAII CATG 7 cut(s) 146, 336, 347, 450, 894, 1260, 1380
DdeI CTNAG 2 cut(s) 123, 573
DpnI GATC 3 cut(s) 8, 75, 620
DpnII GATC 3 cut(s) 6, 73, 618
DraI TTTAAA 1 cut(s) 481
DraIII CACNNNGTG 1 cut(s) 338
Eam1104I CTCTTC 2 cut(s) 29, 122
EarI CTCTTC 2 cut(s) 29, 122
Ecl136II GAGCTC 1 cut(s) 223
Eco130I CCWWGG 3 cut(s) 1259, 1379, 1566
Eco24I GRGCYC 1 cut(s) 225
Eco47I GGWCC 1 cut(s) 501
Eco53kI GAGCTC 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 452
EcoICRI GAGCTC 1 cut(s) 223
EcoT14I CCWWGG 3 cut(s) 1259, 1379, 1566
EcoT38I GRGCYC 1 cut(s) 225
ErhI CCWWGG 3 cut(s) 1259, 1379, 1566
FaeI CATG 7 cut(s) 149, 339, 350, 453, 897, 1263, 1383
FaqI GGGAC 8 cut(s) 638, 671, 704, 770, 803, 986, 1019, 1184
FatI CATG 7 cut(s) 145, 335, 346, 449, 893, 1259, 1379
FauI CCCGC 1 cut(s) 185
FbaI TGATCA 1 cut(s) 73
FblI GTMKAC 3 cut(s) 759, 915, 1140
Fnu4HI GCNGC 3 cut(s) 38, 342, 770
FokI GGATG 4 cut(s) 239, 498, 948, 1506
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 3 cut(s) 38, 342, 770
GluI GCNGC 3 cut(s) 38, 342, 770
HaeIII GGCC 4 cut(s) 185, 215, 329, 472
HapII CCGG 5 cut(s) 216, 845, 1097, 1130, 1163
Hin1II CATG 7 cut(s) 149, 339, 350, 453, 897, 1263, 1383
HinfI GANTC 2 cut(s) 359, 1510
HpaII CCGG 5 cut(s) 216, 845, 1097, 1130, 1163
HphI GGTGA 1 cut(s) 432
Hpy188I TCNGA 1 cut(s) 47
Hpy188III TCNNGA 3 cut(s) 202, 347, 1483
HpyAV CCTTC 1 cut(s) 286
HpyCH4III ACNGT 3 cut(s) 85, 119, 392
HpyCH4V TGCA 4 cut(s) 180, 308, 323, 453
HpyF10VI GCNNNNNNNGC 3 cut(s) 1126, 1159, 1192
HpyF3I CTNAG 2 cut(s) 123, 573
Hsp92II CATG 7 cut(s) 149, 339, 350, 453, 897, 1263, 1383
KroI GCCGGC 1 cut(s) 215
KroNI GCCGGC 1 cut(s) 217
Ksp22I TGATCA 1 cut(s) 73
Kzo9I GATC 3 cut(s) 6, 73, 618
LguI GCTCTTC 1 cut(s) 122
LmnI GCTCC 5 cut(s) 47, 318, 777, 1191, 1224
Lsp1109I GCAGC 2 cut(s) 24, 328
MaeIII GTNAC 1 cut(s) 166
MalI GATC 3 cut(s) 8, 75, 620
MbiI CCGCTC 3 cut(s) 772, 1186, 1219
MboI GATC 3 cut(s) 6, 73, 618
MboII GAAGA 3 cut(s) 16, 16, 139
MflI RGATCY 2 cut(s) 6, 618
MhlI GDGCHC 3 cut(s) 198, 225, 992
MluCI AATT 3 cut(s) 414, 531, 554
MroNI GCCGGC 1 cut(s) 215
MseI TTAA 5 cut(s) 135, 270, 480, 1530, 1573
MslI CAYNNNNRTG 4 cut(s) 368, 448, 1258, 1378
MspI CCGG 5 cut(s) 216, 845, 1097, 1130, 1163
MwoI GCNNNNNNNGC 3 cut(s) 1126, 1159, 1192
NaeI GCCGGC 1 cut(s) 217
NcoI CCATGG 2 cut(s) 1259, 1379
NdeII GATC 3 cut(s) 6, 73, 618
NgoMIV GCCGGC 1 cut(s) 215
NlaIII CATG 7 cut(s) 149, 339, 350, 453, 897, 1263, 1383
NlaIV GGNNCC 4 cut(s) 214, 1101, 1134, 1167
NspI RCATGY 1 cut(s) 453
OliI CACNNNNGTG 1 cut(s) 368
PagI TCATGA 1 cut(s) 346
PciSI GCTCTTC 1 cut(s) 122
PdiI GCCGGC 1 cut(s) 217
PfeI GAWTC 2 cut(s) 359, 1510
PinAI ACCGGT 1 cut(s) 1096
PkrI GCNGC 3 cut(s) 39, 343, 771
Psp124BI GAGCTC 1 cut(s) 225
PspN4I GGNNCC 4 cut(s) 214, 1101, 1134, 1167
PspPI GGNCC 2 cut(s) 213, 501
PstNI CAGNNNCTG 1 cut(s) 1037
PsuI RGATCY 2 cut(s) 6, 618
RseI CAYNNNNRTG 4 cut(s) 368, 448, 1258, 1378
SacI GAGCTC 1 cut(s) 225
SapI GCTCTTC 1 cut(s) 122
SaqAI TTAA 5 cut(s) 135, 270, 480, 1530, 1573
SatI GCNGC 3 cut(s) 38, 342, 770
Sau3AI GATC 3 cut(s) 6, 73, 618
Sau96I GGNCC 2 cut(s) 213, 501
SduI GDGCHC 3 cut(s) 198, 225, 992
SinI GGWCC 1 cut(s) 501
SmiMI CAYNNNNRTG 4 cut(s) 368, 448, 1258, 1378
Sse9I AATT 3 cut(s) 414, 531, 554
SsiI CCGC 9 cut(s) 192, 770, 1118, 1127, 1151, 1160, 1184, 1193, 1217
SstI GAGCTC 1 cut(s) 225
StyI CCWWGG 3 cut(s) 1259, 1379, 1566
TaaI ACNGT 3 cut(s) 85, 119, 392
TaqI TCGA 2 cut(s) 201, 225
TasI AATT 3 cut(s) 414, 531, 554
TatI WGTACW 7 cut(s) 110, 1008, 1041, 1074, 1107, 1173, 1206
TauI GCSGC 1 cut(s) 772
TfiI GAWTC 2 cut(s) 359, 1510
Tru1I TTAA 5 cut(s) 135, 270, 480, 1530, 1573
Tru9I TTAA 5 cut(s) 135, 270, 480, 1530, 1573
TscAI CASTG 6 cut(s) 397, 846, 1011, 1077, 1110, 1209
TseI GCWGC 2 cut(s) 37, 341
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 6 cut(s) 397, 846, 1011, 1077, 1110, 1209
VpaK11BI GGWCC 1 cut(s) 501
XapI RAATTY 1 cut(s) 554
XceI RCATGY 1 cut(s) 453
XcmI CCANNNNNNNNNTGG 1 cut(s) 495
XmiI GTMKAC 3 cut(s) 759, 915, 1140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.