RchiOBHm_Chr6g0245161

Proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0010868
Physical Location & Seq
Reverse (-)
1100839 .. 1103305
2467 bp
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UTR
Exon/CDS
Intron
PRQ21972

Sequence Viewer

Length: 1641 bp
ATGAAGATCTCTCATCTGTGCCTCTTCTTGTCCTTGCTGCTTTTCGGAGCGAGCAGCTTTTGCTATGCTGATGATGATAAGACAGTTGAGGTAGTCGGGTTTGGAGAATGTACTGACTGTGCTGAGAAGAGCATTAAGACCAGTCATGCTTTTTCAGGTCTTGGTGTTACGATCGACTGCAAGGCCGAAAGCGGCCACTTCGAGAGGAGAGGGGCCGGCGAGCTCAATGAGGAGGGCAAGTTTTCAGTGTCCATCCCAAAGGAGATTGTGAAAGATGGAGGAGATGGACTGAAGGAGGAGTGCTATGCACAGCTCCACAATGCAGAGGCCACACCATGTGCTGCTCATGATGGACTTGAATCCACCAAAGTGGTATTCAAGTCTAAAAACAGTGATGGGAAACAAACCTTTGGAATTGTTGGTACTAAACTGAAGTTTTCACCATCTACATGCACTTCTGCTGTCTTTTGGCCTCCCTTTAAACACCCACTTCCCAAATGGACCAAACCACATCCTCTCTTTGGACACCCAATATTCCCTTTCCCTCCAAAAAAATTCCCTCCATTCCCACCTAAGATTACTATTCCTCCATTCTATAAGAAGCCCTGCCCTCCCAAGACCCCTTTTTACGAAAAGCCCCTCCCCCCGCCAGTCCCAATTTACGAAAAGCCCCTCCCCCCACCAGTCCCAATCTACGAAAAGCCCCTCCCTCCCCAGTCCCAATCTACAAGAAGCCACTCCCTCCCCCAGTCCCAATATACAAGAAGCCCCTCCCTCCACCAGTACCAATATACAAGAAGCCGCTCCCTCCCCCAGTCCCAATATACAAGAAGCCCCTCCCTCCACCAGTACCAATATACAAGAAGCCGCTCCCTCCCCCAGTCCCAATATACAAGAAGCCCCTCCCTCCACCAGTCCCAATTTACAAGCCTCCACCAGTGCCGGTTTACAAGAAACCAAACCCTCCACCAGTACCTATCTACAAGAAACCATGCCCTCCACCAGTTCCAGTCTACAAGAAACCCCTCCCTCCACCAGTACCAGTTTACAAAAAGCCCCATCCCCCAAAAGCACCCAAAAAAATGTGCCCTCCACCAGTCCCAGTGTACAAGAAGCCACTCCCTCCACCAGTCCCTGTGTACAAGAAGCCACTCCCTCCACCAGTCCCTGTATACAAAAAGCCACTCCCACCACCAGTCCCTGTGTATAAAAAGCCACTCCCACCACCAGTTCCAGTTTACAAAAAGCCACTCCCACCACCTGTTCCAGTGTACACAAAACCGCTTCCACCGCCGGTTCCTGTGTACACAAAACCGCTCCCACCACCGGTCCCAGTGTACACAAAACCACTCCCACCTCCAATCCCATTTTACAAACCAAAGCCACACCCATTTTACAAACCAAAACCACTTCCACCTCCAATTCCATTTTACAAACCAAAGCCACACCCATGGTTCAAGCCAAAGCCACTTCCACCTCCAATTCCATTTTACAAACCAAAGCCACACCCATGGTTCAAGCCAAAGCCACACCCATTTTACAAACCAAAACCACTTCCACCTCCAATCCCATTTTACAAACCAAAGCCACACCCCTGGTTCAAGCCAAAGCCACACCCATTTTTCAAGCACCCATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

546

Amino Acids

62.7

Weight (kDa)

9.64

Isoelectric Point (pI)

60.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 31 - 116 5.9e-10 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010868)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21140 AT4G38770
fragaria_vesca FvH4_2g01970
malus_domestica MD05G1116400.v1.1 MD10G1118600.v1.1
prunus_persica Prupe.8G160900_v2.0.a1
pyrus_communis pycom05g11060 pycom10g10270
rosa_chinensis RchiOBHm_Chr6g0245161
rosa_laevigata RLG00000015320
rosa_multiflora Rmu_sc0001288.1_g000002 Rmu_sc0015012.1_g000001 Rmu_ssc0000087.1_g000001
rosa_roxburghii Rroxscaffold_7G00215400
rosa_rugosa Rorug05G0512400
rosa_samantha Rh6AG027500 Rh6BG023600 Rh6CG022000 Rh6DG022100
rosa_wichuraiana Rw6G002320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 3 cut(s) 804, 870, 1317
AccI GTMKAC 2 cut(s) 1013, 1172
AciI CCGC 7 cut(s) 192, 647, 802, 868, 1282, 1291, 1315
AcoI YGGCCR 1 cut(s) 193
AcsI RAATTY 1 cut(s) 554
AcuI CTGAAG 2 cut(s) 311, 452
AdeI CACNNNGTG 1 cut(s) 338
AfiI CCNNNNNNNGG 2 cut(s) 521, 1326
AgeI ACCGGT 1 cut(s) 1326
AgsI TTSAA 6 cut(s) 359, 379, 1458, 1518, 1602, 1626
AjnI CCWGG 1 cut(s) 1594
AjuI GAANNNNNNNTTGG 2 cut(s) 359, 391
AleI CACNNNNGTG 1 cut(s) 368
AluBI AGCT 3 cut(s) 57, 223, 313
AluI AGCT 3 cut(s) 57, 223, 313
Alw21I GWGCWC 1 cut(s) 225
AlwNI CAGNNNCTG 3 cut(s) 1135, 1168, 1201
AoxI GGCC 5 cut(s) 183, 193, 213, 327, 470
ApeKI GCWGC 3 cut(s) 37, 54, 341
ApoI RAATTY 1 cut(s) 554
AsiGI ACCGGT 1 cut(s) 1326
AspS9I GGNCC 3 cut(s) 213, 501, 1329
AsuHPI GGTGA 1 cut(s) 432
AvaII GGWCC 2 cut(s) 501, 1329
BaeGI GKGCMC 1 cut(s) 1090
BanII GRGCYC 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 3 cut(s) 24, 66, 328
BccI CCATC 7 cut(s) 260, 269, 278, 344, 389, 451, 1067
BciT130I CCWGG 1 cut(s) 1596
BglII AGATCT 1 cut(s) 6
BisI GCNGC 6 cut(s) 38, 55, 193, 342, 802, 868
BlsI GCNGC 6 cut(s) 39, 56, 194, 343, 803, 869
Bme1390I CCNGG 1 cut(s) 1596
Bme18I GGWCC 2 cut(s) 501, 1329
BmgT120I GGNCC 3 cut(s) 213, 501, 1329
BmiI GGNNCC 3 cut(s) 214, 1298, 1331
BmrFI CCNGG 1 cut(s) 1596
BmrI ACTGGG 6 cut(s) 709, 742, 808, 874, 1096, 1327
BmuI ACTGGG 6 cut(s) 709, 742, 808, 874, 1096, 1327
BsaJI CCNNGG 3 cut(s) 1450, 1510, 1594
BsaWI WCCGGW 1 cut(s) 1326
BsaXI ACNNNNNCTCC 4 cut(s) 224, 254, 571, 601
Bsc4I CCNNNNNNNGG 2 cut(s) 521, 1326
Bse118I RCCGGY 4 cut(s) 215, 942, 1293, 1326
BseBI CCWGG 1 cut(s) 1596
BseDI CCNNGG 3 cut(s) 1450, 1510, 1594
BseGI GGATG 3 cut(s) 252, 511, 1059
BseLI CCNNNNNNNGG 2 cut(s) 521, 1326
BseMII CTCAG 1 cut(s) 114
BseRI GAGGAG 4 cut(s) 220, 245, 294, 311
BseSI GKGCMC 1 cut(s) 1090
BseXI GCAGC 3 cut(s) 24, 66, 328
Bsh1285I CGRYCG 1 cut(s) 174
BshFI GGCC 5 cut(s) 185, 195, 215, 329, 472
BshTI ACCGGT 1 cut(s) 1326
BsiEI CGRYCG 1 cut(s) 174
BsiHKAI GWGCWC 1 cut(s) 225
BsiSI CCGG 4 cut(s) 216, 943, 1294, 1327
BslI CCNNNNNNNGG 2 cut(s) 521, 1326
BsnI GGCC 5 cut(s) 185, 195, 215, 329, 472
Bsp1286I GDGCHC 2 cut(s) 225, 1090
Bsp1407I TGTACA 5 cut(s) 1106, 1139, 1271, 1304, 1337
Bsp143I GATC 2 cut(s) 6, 171
Bsp19I CCATGG 2 cut(s) 1450, 1510
BspACI CCGC 7 cut(s) 192, 647, 802, 868, 1282, 1291, 1315
BspANI GGCC 5 cut(s) 185, 195, 215, 329, 472
BspCNI CTCAG 1 cut(s) 115
BspHI TCATGA 1 cut(s) 346
BspLI GGNNCC 3 cut(s) 214, 1298, 1331
BspQI GCTCTTC 1 cut(s) 122
BsrBI CCGCTC 3 cut(s) 804, 870, 1317
BsrFI RCCGGY 4 cut(s) 215, 942, 1293, 1326
BsrGI TGTACA 5 cut(s) 1106, 1139, 1271, 1304, 1337
BssAI RCCGGY 4 cut(s) 215, 942, 1293, 1326
BssECI CCNNGG 3 cut(s) 1450, 1510, 1594
BssMI GATC 2 cut(s) 6, 171
BssNAI GTATAC 1 cut(s) 1173
BssT1I CCWWGG 2 cut(s) 1450, 1510
Bst1107I GTATAC 1 cut(s) 1173
Bst2UI CCWGG 1 cut(s) 1596
Bst4CI ACNGT 3 cut(s) 85, 119, 392
Bst6I CTCTTC 2 cut(s) 29, 122
BstAPI GCANNNNNTGC 1 cut(s) 60
BstAUI TGTACA 5 cut(s) 1106, 1139, 1271, 1304, 1337
BstC8I GCNNGC 3 cut(s) 52, 217, 221
BstDEI CTNAG 2 cut(s) 123, 573
BstDSI CCRYGG 2 cut(s) 1450, 1510
BstF5I GGATG 3 cut(s) 252, 511, 1059
BstKTI GATC 2 cut(s) 9, 174
BstMBI GATC 2 cut(s) 6, 171
BstMCI CGRYCG 1 cut(s) 174
BstMWI GCNNNNNNNGC 2 cut(s) 60, 1290
BstNI CCWGG 1 cut(s) 1596
BstNSI RCATGY 1 cut(s) 453
BstSCI CCNGG 1 cut(s) 1594
BstSLI GKGCMC 1 cut(s) 1090
BstV1I GCAGC 3 cut(s) 24, 66, 328
BstX2I RGATCY 1 cut(s) 6
BstXI CCANNNNNNTGG 4 cut(s) 370, 1451, 1511, 1595
BstYI RGATCY 1 cut(s) 6
BstZ17I GTATAC 1 cut(s) 1173
BsuRI GGCC 5 cut(s) 185, 195, 215, 329, 472
BtgI CCRYGG 2 cut(s) 1450, 1510
BtsCI GGATG 3 cut(s) 252, 511, 1059
BtsIMutI CAGTG 6 cut(s) 252, 397, 944, 1109, 1274, 1340
Cac8I GCNNGC 3 cut(s) 52, 217, 221
CaiI CAGNNNCTG 3 cut(s) 1135, 1168, 1201
CciI TCATGA 1 cut(s) 346
Cfr10I RCCGGY 4 cut(s) 215, 942, 1293, 1326
Cfr13I GGNCC 3 cut(s) 213, 501, 1329
CspAI ACCGGT 1 cut(s) 1326
CviAII CATG 7 cut(s) 146, 336, 347, 450, 992, 1451, 1511
DdeI CTNAG 2 cut(s) 123, 573
DpnI GATC 2 cut(s) 8, 173
DpnII GATC 2 cut(s) 6, 171
DraI TTTAAA 1 cut(s) 481
DraIII CACNNNGTG 1 cut(s) 338
EaeI YGGCCR 1 cut(s) 193
Eam1104I CTCTTC 2 cut(s) 29, 122
EarI CTCTTC 2 cut(s) 29, 122
Ecl136II GAGCTC 1 cut(s) 223
Eco130I CCWWGG 2 cut(s) 1450, 1510
Eco24I GRGCYC 1 cut(s) 225
Eco47I GGWCC 2 cut(s) 501, 1329
Eco53kI GAGCTC 1 cut(s) 223
Eco57I CTGAAG 2 cut(s) 311, 452
EcoICRI GAGCTC 1 cut(s) 223
EcoRII CCWGG 1 cut(s) 1594
EcoT14I CCWWGG 2 cut(s) 1450, 1510
EcoT38I GRGCYC 1 cut(s) 225
ErhI CCWWGG 2 cut(s) 1450, 1510
FaeI CATG 7 cut(s) 149, 339, 350, 453, 995, 1454, 1514
FatI CATG 7 cut(s) 145, 335, 346, 449, 991, 1450, 1510
FauI CCCGC 1 cut(s) 654
FblI GTMKAC 2 cut(s) 1013, 1172
Fnu4HI GCNGC 6 cut(s) 38, 55, 193, 342, 802, 868
FokI GGATG 3 cut(s) 239, 498, 1046
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 6 cut(s) 38, 55, 193, 342, 802, 868
GluI GCNGC 6 cut(s) 38, 55, 193, 342, 802, 868
HaeIII GGCC 5 cut(s) 185, 195, 215, 329, 472
HapII CCGG 4 cut(s) 216, 943, 1294, 1327
Hin1II CATG 7 cut(s) 149, 339, 350, 453, 995, 1454, 1514
HinfI GANTC 1 cut(s) 359
HpaII CCGG 4 cut(s) 216, 943, 1294, 1327
HphI GGTGA 1 cut(s) 432
Hpy188I TCNGA 1 cut(s) 47
Hpy188III TCNNGA 3 cut(s) 202, 347, 1638
HpyAV CCTTC 1 cut(s) 286
HpyCH4III ACNGT 3 cut(s) 85, 119, 392
HpyCH4V TGCA 4 cut(s) 180, 308, 323, 453
HpyF10VI GCNNNNNNNGC 2 cut(s) 60, 1290
HpyF3I CTNAG 2 cut(s) 123, 573
Hsp92II CATG 7 cut(s) 149, 339, 350, 453, 995, 1454, 1514
KroI GCCGGC 1 cut(s) 215
KroNI GCCGGC 1 cut(s) 217
Kzo9I GATC 2 cut(s) 6, 171
LguI GCTCTTC 1 cut(s) 122
LmnI GCTCC 5 cut(s) 47, 318, 809, 875, 1322
Lsp1109I GCAGC 3 cut(s) 24, 66, 328
MaeIII GTNAC 1 cut(s) 166
MalI GATC 2 cut(s) 8, 173
MbiI CCGCTC 3 cut(s) 804, 870, 1317
MboI GATC 2 cut(s) 6, 171
MboII GAAGA 3 cut(s) 16, 16, 139
MflI RGATCY 1 cut(s) 6
MhlI GDGCHC 2 cut(s) 225, 1090
MluCI AATT 6 cut(s) 414, 554, 657, 920, 1421, 1481
MroNI GCCGGC 1 cut(s) 215
MseI TTAA 2 cut(s) 135, 480
MslI CAYNNNNRTG 4 cut(s) 368, 448, 1449, 1509
MspI CCGG 4 cut(s) 216, 943, 1294, 1327
MspR9I CCNGG 1 cut(s) 1596
MvaI CCWGG 1 cut(s) 1596
MwoI GCNNNNNNNGC 2 cut(s) 60, 1290
NaeI GCCGGC 1 cut(s) 217
NcoI CCATGG 2 cut(s) 1450, 1510
NdeII GATC 2 cut(s) 6, 171
NgoMIV GCCGGC 1 cut(s) 215
NlaIII CATG 7 cut(s) 149, 339, 350, 453, 995, 1454, 1514
NlaIV GGNNCC 3 cut(s) 214, 1298, 1331
NspI RCATGY 1 cut(s) 453
OliI CACNNNNGTG 1 cut(s) 368
PagI TCATGA 1 cut(s) 346
PciSI GCTCTTC 1 cut(s) 122
PdiI GCCGGC 1 cut(s) 217
PfeI GAWTC 1 cut(s) 359
PinAI ACCGGT 1 cut(s) 1326
PkrI GCNGC 6 cut(s) 39, 56, 194, 343, 803, 869
Ple19I CGATCG 1 cut(s) 174
Psp124BI GAGCTC 1 cut(s) 225
Psp6I CCWGG 1 cut(s) 1594
PspGI CCWGG 1 cut(s) 1594
PspN4I GGNNCC 3 cut(s) 214, 1298, 1331
PspPI GGNCC 3 cut(s) 213, 501, 1329
PstNI CAGNNNCTG 3 cut(s) 1135, 1168, 1201
PsuI RGATCY 1 cut(s) 6
PvuI CGATCG 1 cut(s) 174
RseI CAYNNNNRTG 4 cut(s) 368, 448, 1449, 1509
SacI GAGCTC 1 cut(s) 225
SapI GCTCTTC 1 cut(s) 122
SaqAI TTAA 2 cut(s) 135, 480
SatI GCNGC 6 cut(s) 38, 55, 193, 342, 802, 868
Sau3AI GATC 2 cut(s) 6, 171
Sau96I GGNCC 3 cut(s) 213, 501, 1329
ScrFI CCNGG 1 cut(s) 1596
SduI GDGCHC 2 cut(s) 225, 1090
SinI GGWCC 2 cut(s) 501, 1329
SmiMI CAYNNNNRTG 4 cut(s) 368, 448, 1449, 1509
Sse9I AATT 6 cut(s) 414, 554, 657, 920, 1421, 1481
SsiI CCGC 7 cut(s) 192, 647, 802, 868, 1282, 1291, 1315
SspI AATATT 1 cut(s) 534
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 1 cut(s) 1594
StyI CCWWGG 2 cut(s) 1450, 1510
TaaI ACNGT 3 cut(s) 85, 119, 392
TaqI TCGA 2 cut(s) 174, 201
TasI AATT 6 cut(s) 414, 554, 657, 920, 1421, 1481
TatI WGTACW 6 cut(s) 110, 1106, 1139, 1271, 1304, 1337
TauI GCSGC 3 cut(s) 195, 804, 870
TfiI GAWTC 1 cut(s) 359
Tru1I TTAA 2 cut(s) 135, 480
Tru9I TTAA 2 cut(s) 135, 480
TscAI CASTG 6 cut(s) 252, 397, 944, 1109, 1274, 1340
TseI GCWGC 3 cut(s) 37, 54, 341
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 6 cut(s) 252, 397, 944, 1109, 1274, 1340
VpaK11BI GGWCC 2 cut(s) 501, 1329
XapI RAATTY 1 cut(s) 554
XceI RCATGY 1 cut(s) 453
XcmI CCANNNNNNNNNTGG 1 cut(s) 495
XmiI GTMKAC 2 cut(s) 1013, 1172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.