FvH4_2g05340

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0013605
Physical Location & Seq
Forward (+)
4388041 .. 4390224
2184 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g05340.t1

Sequence Viewer

Length: 1149 bp
ATGTACAGACCGGTGATGTTTATGGCCTCAGATCACCAGAGCACAACCCAAGACTCTCCCCTTTTCCAAGACTCCAAAAAACCCATTCCCACCACCACAACCAGTTCTAGCAGCAAAGGAAGATCAAGGAGTTCAGCTAAACCTAAAAGGTCAAGAAAGCCCCAGAGAGGTGTGGGTGTGGCTCAGCTGGAGCGTCTACGTCTCCAGAGAGGTGGAGGTGTGACAAGTTTGAACCAAGTGCAAATCCTACCACATCCTCAACCCCAAACCCAACTTGTCCAAAATGATACTGTCCTTCCGGATCATCATCAACCACGCCAGTTTCAGTACTACTACCAAAACCCACAAACCCGCCCCGACCCGCTTCACAGTGTTCCAGTACTCTACGGTGCGTCGAACTACGATGGAGCCATGACTGGTGGCCCTGGTGTGGTGGTTCAGACCGTTAATGGGAATGGTGGCCAAGGCTCAGCTGGGTTTGGTTATGGGTTTAATGTTCGTCTTCAAAATGATGTTGGTGGGTTGGTCATGGAACCTAACCAGTACGGTGTCGTTGGGGCTGGAGGAACTCAGAATCGCGGGGCTGTGTATGAGATACCAAAGATGAAGCAGCAGCTGCAGCAGCAACCCACGTCTTCTGCTTCATCTGAGTGTTGCTGCTGTTGCAAGCAGATGAAGATTTTCAACTGCGGAAACAACATGGGTTTTGGGGAATTTCCGACTGTGTTTCCGATGGTCAACAGTTCCGATCTTCCCGGATTCGACCCACAAAGCACCCCTAATGTCCGGCTTGCTAGGGAGACCGGCAGTAGTTTCAGTGGTGGTACTTACTTGAGCTGCCGCCCTAGTGTTGTTGATGAGAGTGTGGAGGTTAGGGCAGTACACAGGAAGGGAAGACAAGCAGGTGGTGATATCATGATGGAGTATGAATTCTTTCCTGATCATAACAACAACTCTTCTTCATCAGGCACATATCCCATGGACCGTGAAGTAGTGGCTTCATCAACTAATTCTTCTTCAGTTGGTAGATCATCAGAAGCATCTGATCAACATCTTGGTGCTGCTTGTATTGAGGGGGCTGACATGATGATGATGATGATGAGCAGTGGCTATGGTCCTGTTGATTTATCACTCAAATTGTCTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NOZZLE

Protein Analysis

383

Amino Acids

41.3

Weight (kDa)

8.37

Isoelectric Point (pI)

47.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 893
Acc36I ACCTGC 1 cut(s) 893
AccI GTMKAC 1 cut(s) 196
AccII CGCG 1 cut(s) 579
AccIII TCCGGA 1 cut(s) 298
AciI CCGC 5 cut(s) 352, 362, 579, 690, 841
AclWI GGATC 1 cut(s) 309
AcoI YGGCCR 1 cut(s) 460
AcsI RAATTY 2 cut(s) 713, 929
AcuI CTGAAG 1 cut(s) 1002
AfaI GTAC 6 cut(s) 5, 329, 381, 545, 826, 882
AfiI CCNNNNNNNGG 3 cut(s) 167, 430, 450
AgeI ACCGGT 1 cut(s) 10
AgsI TTSAA 3 cut(s) 232, 506, 685
AjiI CACGTC 1 cut(s) 633
AjnI CCWGG 1 cut(s) 424
AluBI AGCT 5 cut(s) 137, 187, 473, 616, 837
AluI AGCT 5 cut(s) 137, 187, 473, 616, 837
Alw21I GWGCWC 1 cut(s) 44
Alw26I GTCTC 2 cut(s) 206, 794
AlwI GGATC 1 cut(s) 309
AlwNI CAGNNNCTG 1 cut(s) 616
Aor13HI TCCGGA 1 cut(s) 298
AoxI GGCC 3 cut(s) 24, 421, 460
ApeKI GCWGC 9 cut(s) 111, 610, 613, 616, 619, 622, 657, 837, 1061
ApoI RAATTY 2 cut(s) 713, 929
AsiGI ACCGGT 1 cut(s) 10
Asp700I GAANNNNTTC 2 cut(s) 680, 933
AspS9I GGNCC 3 cut(s) 422, 982, 1115
AsuC2I CCSGG 1 cut(s) 756
AsuHPI GGTGA 3 cut(s) 25, 26, 920
AvaII GGWCC 2 cut(s) 982, 1115
BalI TGGCCA 1 cut(s) 462
BbsI GAAGAC 3 cut(s) 494, 627, 901
Bbv12I GWGCWC 1 cut(s) 44
BbvI GCAGC 9 cut(s) 123, 603, 622, 625, 631, 634, 644, 824, 1048
BccI CCATC 3 cut(s) 398, 727, 913
BciT130I CCWGG 1 cut(s) 426
BclI TGATCA 2 cut(s) 940, 1045
BcnI CCSGG 1 cut(s) 756
BcoDI GTCTC 2 cut(s) 206, 794
BfaI CTAG 4 cut(s) 108, 795, 846, 1147
BfmI CTRYAG 1 cut(s) 617
BfuAI ACCTGC 1 cut(s) 893
BlpI GCTNAGC 2 cut(s) 183, 469
BmcAI AGTACT 2 cut(s) 329, 381
Bme1390I CCNGG 2 cut(s) 426, 756
Bme18I GGWCC 2 cut(s) 982, 1115
BmgBI CACGTC 1 cut(s) 633
BmgT120I GGNCC 3 cut(s) 422, 982, 1115
BmiI GGNNCC 2 cut(s) 409, 534
BmrFI CCNGG 2 cut(s) 426, 756
BmsI GCATC 1 cut(s) 1049
BpiI GAAGAC 3 cut(s) 494, 627, 901
BpmI CTGGAG 3 cut(s) 188, 209, 582
Bpu1102I GCTNAGC 2 cut(s) 183, 469
BpuEI CTTGAG 1 cut(s) 853
BpuMI CCSGG 1 cut(s) 756
BsaBI GATNNNNATC 2 cut(s) 306, 1050
BsaI GGTCTC 1 cut(s) 794
BsaJI CCNNGG 3 cut(s) 424, 463, 978
BsaWI WCCGGW 2 cut(s) 10, 298
Bsc4I CCNNNNNNNGG 3 cut(s) 167, 430, 450
Bse118I RCCGGY 2 cut(s) 10, 803
Bse1I ACTGG 5 cut(s) 102, 319, 377, 421, 541
Bse8I GATNNNNATC 2 cut(s) 306, 1050
BseAI TCCGGA 1 cut(s) 298
BseBI CCWGG 1 cut(s) 426
BseDI CCNNGG 3 cut(s) 424, 463, 978
BseGI GGATG 1 cut(s) 253
BseJI GATNNNNATC 2 cut(s) 306, 1050
BseLI CCNNNNNNNGG 3 cut(s) 167, 430, 450
BseMII CTCAG 5 cut(s) 42, 197, 483, 584, 639
BseNI ACTGG 5 cut(s) 102, 319, 377, 421, 541
BseXI GCAGC 9 cut(s) 123, 603, 622, 625, 631, 634, 644, 824, 1048
BseYI CCCAGC 1 cut(s) 473
Bsh1236I CGCG 1 cut(s) 579
BshFI GGCC 3 cut(s) 26, 423, 462
BshTI ACCGGT 1 cut(s) 10
BsiHKAI GWGCWC 1 cut(s) 44
BsiSI CCGG 5 cut(s) 11, 299, 756, 787, 804
BslI CCNNNNNNNGG 3 cut(s) 167, 430, 450
BsmAI GTCTC 2 cut(s) 206, 794
BsmBI CGTCTC 1 cut(s) 206
BsnI GGCC 3 cut(s) 26, 423, 462
Bso31I GGTCTC 1 cut(s) 794
Bsp1286I GDGCHC 1 cut(s) 44
Bsp13I TCCGGA 1 cut(s) 298
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 7 cut(s) 31, 122, 301, 748, 940, 1028, 1045
Bsp1720I GCTNAGC 2 cut(s) 183, 469
Bsp19I CCATGG 1 cut(s) 978
BspACI CCGC 5 cut(s) 352, 362, 579, 690, 841
BspANI GGCC 3 cut(s) 26, 423, 462
BspCNI CTCAG 5 cut(s) 41, 196, 482, 583, 640
BspEI TCCGGA 1 cut(s) 298
BspFNI CGCG 1 cut(s) 579
BspHI TCATGA 1 cut(s) 915
BspLI GGNNCC 2 cut(s) 409, 534
BspMAI CTGCAG 1 cut(s) 621
BspMI ACCTGC 1 cut(s) 893
BspPI GGATC 1 cut(s) 309
BspTNI GGTCTC 1 cut(s) 794
BsrFI RCCGGY 2 cut(s) 10, 803
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 5 cut(s) 102, 319, 377, 421, 541
BssAI RCCGGY 2 cut(s) 10, 803
BssECI CCNNGG 3 cut(s) 424, 463, 978
BssMI GATC 7 cut(s) 31, 122, 301, 748, 940, 1028, 1045
BssT1I CCWWGG 2 cut(s) 463, 978
Bst2UI CCWGG 1 cut(s) 426
Bst4CI ACNGT 8 cut(s) 292, 371, 389, 445, 548, 724, 743, 986
Bst6I CTCTTC 1 cut(s) 961
BstAPI GCANNNNNTGC 1 cut(s) 616
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 2 cut(s) 668, 792
BstDEI CTNAG 5 cut(s) 28, 183, 469, 570, 648
BstDSI CCRYGG 1 cut(s) 978
BstF5I GGATG 1 cut(s) 253
BstFNI CGCG 1 cut(s) 579
BstKTI GATC 7 cut(s) 34, 125, 304, 751, 943, 1031, 1048
BstMAI GTCTC 2 cut(s) 206, 794
BstMBI GATC 7 cut(s) 31, 122, 301, 748, 940, 1028, 1045
BstMWI GCNNNNNNNGC 4 cut(s) 616, 619, 622, 663
BstNI CCWGG 1 cut(s) 426
BstSCI CCNGG 2 cut(s) 424, 754
BstSFI CTRYAG 1 cut(s) 617
BstUI CGCG 1 cut(s) 579
BstV1I GCAGC 9 cut(s) 123, 603, 622, 625, 631, 634, 644, 824, 1048
BstV2I GAAGAC 3 cut(s) 494, 627, 901
BstXI CCANNNNNNTGG 1 cut(s) 212
BsuRI GGCC 3 cut(s) 26, 423, 462
BtgI CCRYGG 1 cut(s) 978
BtrI CACGTC 1 cut(s) 633
BtsCI GGATG 1 cut(s) 253
BtsI GCAGTG 1 cut(s) 1111
BtsIMutI CAGTG 3 cut(s) 376, 823, 1111
BveI ACCTGC 1 cut(s) 893
Cac8I GCNNGC 2 cut(s) 668, 792
CaiI CAGNNNCTG 1 cut(s) 616
CciI TCATGA 1 cut(s) 915
Cfr10I RCCGGY 2 cut(s) 10, 803
Cfr13I GGNCC 3 cut(s) 422, 982, 1115
CseI GACGC 2 cut(s) 182, 381
Csp6I GTAC 6 cut(s) 4, 328, 380, 544, 825, 881
CspAI ACCGGT 1 cut(s) 10
CviAII CATG 6 cut(s) 412, 529, 700, 916, 979, 1084
CviQI GTAC 6 cut(s) 4, 328, 380, 544, 825, 881
DdeI CTNAG 5 cut(s) 28, 183, 469, 570, 648
DpnI GATC 7 cut(s) 33, 124, 303, 750, 942, 1030, 1047
DpnII GATC 7 cut(s) 31, 122, 301, 748, 940, 1028, 1045
EaeI YGGCCR 1 cut(s) 460
Eam1104I CTCTTC 1 cut(s) 961
EarI CTCTTC 1 cut(s) 961
Eco130I CCWWGG 2 cut(s) 463, 978
Eco31I GGTCTC 1 cut(s) 794
Eco32I GATATC 1 cut(s) 913
Eco47I GGWCC 2 cut(s) 982, 1115
Eco57I CTGAAG 1 cut(s) 1002
EcoRI GAATTC 1 cut(s) 929
EcoRII CCWGG 1 cut(s) 424
EcoRV GATATC 1 cut(s) 913
EcoT14I CCWWGG 2 cut(s) 463, 978
ErhI CCWWGG 2 cut(s) 463, 978
Esp3I CGTCTC 1 cut(s) 206
FaeI CATG 6 cut(s) 415, 532, 703, 919, 982, 1087
FatI CATG 6 cut(s) 411, 528, 699, 915, 978, 1083
FauI CCCGC 3 cut(s) 359, 369, 572
FbaI TGATCA 2 cut(s) 940, 1045
FblI GTMKAC 1 cut(s) 196
FokI GGATG 1 cut(s) 240
FspBI CTAG 4 cut(s) 108, 795, 846, 1147
GsaI CCCAGC 1 cut(s) 477
GsuI CTGGAG 3 cut(s) 188, 209, 582
HaeIII GGCC 3 cut(s) 26, 423, 462
HapII CCGG 5 cut(s) 11, 299, 756, 787, 804
HgaI GACGC 2 cut(s) 182, 381
Hin1II CATG 6 cut(s) 415, 532, 703, 919, 982, 1087
HincII GTYRAC 1 cut(s) 739
HindII GTYRAC 1 cut(s) 739
HinfI GANTC 4 cut(s) 53, 71, 574, 759
HpaII CCGG 5 cut(s) 11, 299, 756, 787, 804
HphI GGTGA 3 cut(s) 25, 26, 920
Hpy166II GTNNAC 3 cut(s) 197, 739, 883
Hpy188I TCNGA 9 cut(s) 31, 441, 573, 649, 720, 732, 748, 1036, 1045
Hpy188III TCNNGA 5 cut(s) 153, 205, 299, 916, 938
Hpy8I GTNNAC 3 cut(s) 197, 739, 883
Hpy99I CGWCG 1 cut(s) 397
HpyAV CCTTC 2 cut(s) 305, 883
HpyCH4III ACNGT 8 cut(s) 292, 371, 389, 445, 548, 724, 743, 986
HpyCH4IV ACGT 2 cut(s) 199, 632
HpyCH4V TGCA 3 cut(s) 241, 619, 666
HpyF10VI GCNNNNNNNGC 4 cut(s) 616, 619, 622, 663
HpyF3I CTNAG 5 cut(s) 28, 183, 469, 570, 648
HpySE526I ACGT 2 cut(s) 199, 632
Hsp92II CATG 6 cut(s) 415, 532, 703, 919, 982, 1087
Kpn2I TCCGGA 1 cut(s) 298
Ksp22I TGATCA 2 cut(s) 940, 1045
Kzo9I GATC 7 cut(s) 31, 122, 301, 748, 940, 1028, 1045
LmnI GCTCC 2 cut(s) 190, 407
Lsp1109I GCAGC 9 cut(s) 123, 603, 622, 625, 631, 634, 644, 824, 1048
LweI GCATC 1 cut(s) 1049
MaeI CTAG 4 cut(s) 108, 795, 846, 1147
MaeII ACGT 2 cut(s) 199, 632
MaeIII GTNAC 1 cut(s) 220
MalI GATC 7 cut(s) 33, 124, 303, 750, 942, 1030, 1047
MboI GATC 7 cut(s) 31, 122, 301, 748, 940, 1028, 1045
MhlI GDGCHC 1 cut(s) 44
MlsI TGGCCA 1 cut(s) 462
MluCI AATT 4 cut(s) 713, 929, 1009, 1136
MluNI TGGCCA 1 cut(s) 462
MlyI GAGTC 2 cut(s) 47, 65
MmeI TCCRAC 1 cut(s) 743
MnlI CCTC 8 cut(s) 37, 161, 203, 209, 267, 557, 862, 1066
Mox20I TGGCCA 1 cut(s) 462
MroI TCCGGA 1 cut(s) 298
MroXI GAANNNNTTC 2 cut(s) 680, 933
MscI TGGCCA 1 cut(s) 462
MseI TTAA 2 cut(s) 447, 492
MslI CAYNNNNRTG 3 cut(s) 649, 1056, 1088
Msp20I TGGCCA 1 cut(s) 462
MspA1I CMGCKG 3 cut(s) 187, 473, 616
MspI CCGG 5 cut(s) 11, 299, 756, 787, 804
MspR9I CCNGG 2 cut(s) 426, 756
MvaI CCWGG 1 cut(s) 426
MvnI CGCG 1 cut(s) 579
MwoI GCNNNNNNNGC 4 cut(s) 616, 619, 622, 663
NciI CCSGG 1 cut(s) 756
NcoI CCATGG 1 cut(s) 978
NdeII GATC 7 cut(s) 31, 122, 301, 748, 940, 1028, 1045
NlaIII CATG 6 cut(s) 415, 532, 703, 919, 982, 1087
NlaIV GGNNCC 2 cut(s) 409, 534
NmuCI GTSAC 1 cut(s) 220
PagI TCATGA 1 cut(s) 915
PaqCI CACCTGC 1 cut(s) 893
PdmI GAANNNNTTC 2 cut(s) 680, 933
PfeI GAWTC 2 cut(s) 574, 759
PfoI TCCNGGA 1 cut(s) 754
PinAI ACCGGT 1 cut(s) 10
PleI GAGTC 2 cut(s) 47, 65
PpsI GAGTC 2 cut(s) 47, 65
Psp6I CCWGG 1 cut(s) 424
PspFI CCCAGC 1 cut(s) 473
PspGI CCWGG 1 cut(s) 424
PspN4I GGNNCC 2 cut(s) 409, 534
PspPI GGNCC 3 cut(s) 422, 982, 1115
PstI CTGCAG 1 cut(s) 621
PstNI CAGNNNCTG 1 cut(s) 616
PvuII CAGCTG 3 cut(s) 187, 473, 616
RsaI GTAC 6 cut(s) 5, 329, 381, 545, 826, 882
RsaNI GTAC 6 cut(s) 4, 328, 380, 544, 825, 881
RseI CAYNNNNRTG 3 cut(s) 649, 1056, 1088
SaqAI TTAA 2 cut(s) 447, 492
Sau3AI GATC 7 cut(s) 31, 122, 301, 748, 940, 1028, 1045
Sau96I GGNCC 3 cut(s) 422, 982, 1115
ScaI AGTACT 2 cut(s) 329, 381
SchI GAGTC 2 cut(s) 47, 65
ScrFI CCNGG 2 cut(s) 426, 756
SduI GDGCHC 1 cut(s) 44
SfaNI GCATC 1 cut(s) 1049
SfcI CTRYAG 1 cut(s) 617
SinI GGWCC 2 cut(s) 982, 1115
SmiMI CAYNNNNRTG 3 cut(s) 649, 1056, 1088
SmlI CTYRAG 1 cut(s) 832
SmoI CTYRAG 1 cut(s) 832
Sse9I AATT 4 cut(s) 713, 929, 1009, 1136
SsiI CCGC 5 cut(s) 352, 362, 579, 690, 841
SspMI CTAG 4 cut(s) 108, 795, 846, 1147
StyD4I CCNGG 2 cut(s) 424, 754
StyI CCWWGG 2 cut(s) 463, 978
TaaI ACNGT 8 cut(s) 292, 371, 389, 445, 548, 724, 743, 986
TaiI ACGT 2 cut(s) 202, 635
TaqI TCGA 2 cut(s) 395, 762
TasI AATT 4 cut(s) 713, 929, 1009, 1136
TatI WGTACW 4 cut(s) 3, 327, 379, 880
TauI GCSGC 1 cut(s) 843
TfiI GAWTC 2 cut(s) 574, 759
Tru1I TTAA 2 cut(s) 447, 492
Tru9I TTAA 2 cut(s) 447, 492
TscAI CASTG 3 cut(s) 376, 823, 1111
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 9 cut(s) 111, 610, 613, 616, 619, 622, 657, 837, 1061
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 6 cut(s) 620, 633, 689, 942, 951, 990
TspRI CASTG 3 cut(s) 376, 823, 1111
VpaK11BI GGWCC 2 cut(s) 982, 1115
XapI RAATTY 2 cut(s) 713, 929
XcmI CCANNNNNNNNNTGG 1 cut(s) 470
XmiI GTMKAC 1 cut(s) 196
XmnI GAANNNNTTC 2 cut(s) 680, 933
XspI CTAG 4 cut(s) 108, 795, 846, 1147
ZrmI AGTACT 2 cut(s) 329, 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.