Rw6G006710

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0013605
Physical Location & Seq
Forward (+)
10763103 .. 10764620
1518 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G006710.1

Sequence Viewer

Length: 1200 bp
ATGTACAGACCGGTGATGCTCATGGCCTCAGATTACCAGAGCACAACCCAAGATTCTTCCCGTTTTGGGTGCGAAGAATCCAAAAAACCCGAAACCACCACCAAAACCAGTACTAGCAGCAAAGAAAAATCAAGGAAGTCAGCTAAACCTAAAAGGCCAAAGCCCCAGCGAGGTGTTGGTGTGGCTCAGCTGGAGCGCCTCCGTCTCCAACAGAGAGGTGTGGGTGTGACCAAATTAACCCAAATGCCCCTCCCCCATATCCTACCGCAACCTCAACCTCAACCTCAACCTCAGACCCAAGTTGTCCAAAATGTTGGTTTTCCAGATCACCAGCAGCGCCAGTTTCAATACTACCCAAACCCAGAAACCCGCGCCGACCCGCTTCTCAGCATTCCGGTACTCTACGGTGCGTCCAACTACGATGGAGCCATGAGTACTGGCTCTGGTTTGGTGGGCTTGGACCGGACCGGTTTGTTAGTTCAGACAGTTAATGGGAATGGTGGTTCAGCTGGGTTTGGTTATGGGTACAATGTTCGTCTTCATAATGATGTTGGTGGGTTGGTTATGGATCCGAACCAGTACGGTGTCGTTGGGGCTGGGGCTCCGAATCGTGGGGCTGTGTATGAGATCTCGAACGAGCTCTCTTCAATACCAAAGATGAAGCAGCAACCCTCCTCTTCTTCTTCATCTGAATATTGCTGCTGTTGCAAGCAGAAGAAAAGATTCAATTGTGGAAACAACATGGGTTTTGGGGAATCTCCGGCTGTCTTTCCGATGGTCAACAGTTCTGATCTTCCCGGATTCGACCAACAAAACATCCCAAATGTCCGGGTAGCTGGGGAAATCGGCGTTGGTTTCAATGCTGGTACTTACTTGGACCGCCGCTCCAGTCTTGTTGATGAGAGTGTGGAGGTTAGGGCAGTTCACAGGAAGGGGAGACAAGCAGGTGGTGATATCATGAAGGAGTATGAGTTCTTTCCTGATCACAACAACAACTCTTCTTCAGTAGTCTCATCAGGCACATATCCCATGGAGCATAAAATAGTTGCTTCATCAACTAATTCTTCTTCAGTTGGTAGATCATCAGAAGCTTCTGATCAACATCTTGGTGCTGCTTGTATTGAGGGGGCTGACATGATGATGATGATGATGAGCAGTGGCTATAGTCCTGTTGATTTGTCACTCAAATTGTCTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NOZZLE

Protein Analysis

399

Amino Acids

43.22

Weight (kDa)

8.71

Isoelectric Point (pI)

50.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 935
Acc36I ACCTGC 1 cut(s) 935
AccBSI CCGCTC 1 cut(s) 885
AccII CGCG 1 cut(s) 372
AciI CCGC 5 cut(s) 266, 370, 380, 880, 883
AclWI GGATC 2 cut(s) 563, 576
AcuI CTGAAG 2 cut(s) 987, 1053
AfaI GTAC 7 cut(s) 5, 112, 399, 436, 527, 581, 868
AfiI CCNNNNNNNGG 3 cut(s) 66, 170, 611
AgeI ACCGGT 2 cut(s) 10, 467
AgsI TTSAA 4 cut(s) 347, 648, 727, 859
AjuI GAANNNNNNNTTGG 2 cut(s) 834, 866
AloI GAACNNNNNNTCC 4 cut(s) 487, 519, 956, 988
AluBI AGCT 6 cut(s) 143, 190, 509, 640, 836, 1091
AluI AGCT 6 cut(s) 143, 190, 509, 640, 836, 1091
Alw21I GWGCWC 2 cut(s) 44, 642
Alw26I GTCTC 3 cut(s) 209, 931, 1015
AlwI GGATC 2 cut(s) 563, 576
AoxI GGCC 2 cut(s) 24, 155
ApeKI GCWGC 5 cut(s) 117, 334, 664, 699, 1112
AsiGI ACCGGT 2 cut(s) 10, 467
Asp700I GAANNNNTTC 1 cut(s) 722
AspLEI GCGC 3 cut(s) 198, 339, 374
AspS9I GGNCC 3 cut(s) 460, 465, 877
AsuC2I CCSGG 2 cut(s) 798, 830
AsuHPI GGTGA 3 cut(s) 25, 320, 962
AvaII GGWCC 3 cut(s) 460, 465, 877
BamHI GGATCC 1 cut(s) 568
BanII GRGCYC 2 cut(s) 604, 642
BbsI GAAGAC 1 cut(s) 530
Bbv12I GWGCWC 2 cut(s) 44, 642
BbvI GCAGC 5 cut(s) 129, 346, 676, 686, 1099
BccI CCATC 2 cut(s) 416, 769
BclI TGATCA 2 cut(s) 982, 1096
BcnI CCSGG 2 cut(s) 798, 830
BcoDI GTCTC 3 cut(s) 209, 931, 1015
BfaI CTAG 2 cut(s) 114, 1198
BfmI CTRYAG 1 cut(s) 1162
BfoI RGCGCY 2 cut(s) 199, 340
BfuAI ACCTGC 1 cut(s) 935
BglII AGATCT 1 cut(s) 627
BisI GCNGC 6 cut(s) 118, 335, 665, 700, 883, 1113
BlpI GCTNAGC 1 cut(s) 186
BlsI GCNGC 6 cut(s) 119, 336, 666, 701, 884, 1114
BmcAI AGTACT 2 cut(s) 112, 436
Bme1390I CCNGG 2 cut(s) 798, 830
Bme18I GGWCC 3 cut(s) 460, 465, 877
BmgT120I GGNCC 3 cut(s) 460, 465, 877
BmiI GGNNCC 3 cut(s) 427, 570, 603
BmrFI CCNGG 2 cut(s) 798, 830
BmsI GCATC 1 cut(s) 6
BpiI GAAGAC 1 cut(s) 530
BpmI CTGGAG 2 cut(s) 212, 871
Bpu1102I GCTNAGC 1 cut(s) 186
BpuMI CCSGG 2 cut(s) 798, 830
BsaBI GATNNNNATC 1 cut(s) 1101
BsaJI CCNNGG 1 cut(s) 1029
BsaWI WCCGGW 4 cut(s) 10, 394, 462, 467
BsaXI ACNNNNNCTCC 4 cut(s) 869, 899, 956, 986
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 170, 611
Bse118I RCCGGY 2 cut(s) 10, 467
Bse1I ACTGG 5 cut(s) 108, 340, 442, 577, 888
Bse8I GATNNNNATC 1 cut(s) 1101
BseDI CCNNGG 1 cut(s) 1029
BseGI GGATG 1 cut(s) 816
BseJI GATNNNNATC 1 cut(s) 1101
BseLI CCNNNNNNNGG 3 cut(s) 66, 170, 611
BseMII CTCAG 4 cut(s) 42, 200, 305, 400
BseNI ACTGG 5 cut(s) 108, 340, 442, 577, 888
BseRI GAGGAG 1 cut(s) 664
BseXI GCAGC 5 cut(s) 129, 346, 676, 686, 1099
BseYI CCCAGC 4 cut(s) 165, 509, 596, 836
Bsh1236I CGCG 1 cut(s) 372
BshFI GGCC 2 cut(s) 26, 157
BshTI ACCGGT 2 cut(s) 10, 467
BsiHKAI GWGCWC 2 cut(s) 44, 642
BsiSI CCGG 7 cut(s) 11, 395, 463, 468, 761, 798, 829
BslI CCNNNNNNNGG 3 cut(s) 66, 170, 611
BsmAI GTCTC 3 cut(s) 209, 931, 1015
BsmBI CGTCTC 1 cut(s) 209
BsmI GAATGC 1 cut(s) 390
BsnI GGCC 2 cut(s) 26, 157
Bsp1286I GDGCHC 3 cut(s) 44, 604, 642
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 7 cut(s) 325, 568, 627, 790, 982, 1079, 1096
Bsp1720I GCTNAGC 1 cut(s) 186
Bsp19I CCATGG 1 cut(s) 1029
BspACI CCGC 5 cut(s) 266, 370, 380, 880, 883
BspANI GGCC 2 cut(s) 26, 157
BspCNI CTCAG 4 cut(s) 41, 199, 304, 399
BspFNI CGCG 1 cut(s) 372
BspHI TCATGA 1 cut(s) 957
BspLI GGNNCC 3 cut(s) 427, 570, 603
BspMI ACCTGC 1 cut(s) 935
BspPI GGATC 2 cut(s) 563, 576
BsrBI CCGCTC 1 cut(s) 885
BsrFI RCCGGY 2 cut(s) 10, 467
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 5 cut(s) 108, 340, 442, 577, 888
BssAI RCCGGY 2 cut(s) 10, 467
BssECI CCNNGG 1 cut(s) 1029
BssMI GATC 7 cut(s) 325, 568, 627, 790, 982, 1079, 1096
BssT1I CCWWGG 1 cut(s) 1029
Bst4CI ACNGT 4 cut(s) 407, 487, 584, 785
Bst6I CTCTTC 3 cut(s) 649, 682, 1003
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 1 cut(s) 710
BstDEI CTNAG 4 cut(s) 28, 186, 291, 386
BstDSI CCRYGG 1 cut(s) 1029
BstF5I GGATG 1 cut(s) 816
BstFNI CGCG 1 cut(s) 372
BstH2I RGCGCY 2 cut(s) 199, 340
BstHHI GCGC 3 cut(s) 198, 339, 374
BstKTI GATC 7 cut(s) 328, 571, 630, 793, 985, 1082, 1099
BstMAI GTCTC 3 cut(s) 209, 931, 1015
BstMBI GATC 7 cut(s) 325, 568, 627, 790, 982, 1079, 1096
BstMWI GCNNNNNNNGC 1 cut(s) 705
BstSCI CCNGG 2 cut(s) 796, 828
BstSFI CTRYAG 1 cut(s) 1162
BstUI CGCG 1 cut(s) 372
BstV1I GCAGC 5 cut(s) 129, 346, 676, 686, 1099
BstV2I GAAGAC 1 cut(s) 530
BstX2I RGATCY 2 cut(s) 568, 627
BstXI CCANNNNNNTGG 1 cut(s) 314
BstYI RGATCY 2 cut(s) 568, 627
BsuRI GGCC 2 cut(s) 26, 157
BtgI CCRYGG 1 cut(s) 1029
BtsCI GGATG 1 cut(s) 816
BtsI GCAGTG 1 cut(s) 1162
BtsIMutI CAGTG 1 cut(s) 1162
BveI ACCTGC 1 cut(s) 935
Cac8I GCNNGC 1 cut(s) 710
CciI TCATGA 1 cut(s) 957
CfoI GCGC 3 cut(s) 198, 339, 374
Cfr10I RCCGGY 2 cut(s) 10, 467
Cfr13I GGNCC 3 cut(s) 460, 465, 877
CpoI CGGWCCG 1 cut(s) 465
CseI GACGC 1 cut(s) 399
Csp6I GTAC 7 cut(s) 4, 111, 398, 435, 526, 580, 867
CspAI ACCGGT 2 cut(s) 10, 467
CspI CGGWCCG 1 cut(s) 465
CviAII CATG 6 cut(s) 22, 430, 742, 958, 1030, 1135
CviQI GTAC 7 cut(s) 4, 111, 398, 435, 526, 580, 867
DdeI CTNAG 4 cut(s) 28, 186, 291, 386
DpnI GATC 7 cut(s) 327, 570, 629, 792, 984, 1081, 1098
DpnII GATC 7 cut(s) 325, 568, 627, 790, 982, 1079, 1096
Eam1104I CTCTTC 3 cut(s) 649, 682, 1003
EarI CTCTTC 3 cut(s) 649, 682, 1003
Ecl136II GAGCTC 1 cut(s) 640
Eco130I CCWWGG 1 cut(s) 1029
Eco24I GRGCYC 2 cut(s) 604, 642
Eco32I GATATC 1 cut(s) 955
Eco47I GGWCC 3 cut(s) 460, 465, 877
Eco53kI GAGCTC 1 cut(s) 640
Eco57I CTGAAG 2 cut(s) 987, 1053
EcoICRI GAGCTC 1 cut(s) 640
EcoRV GATATC 1 cut(s) 955
EcoT14I CCWWGG 1 cut(s) 1029
EcoT38I GRGCYC 2 cut(s) 604, 642
ErhI CCWWGG 1 cut(s) 1029
Esp3I CGTCTC 1 cut(s) 209
FaeI CATG 6 cut(s) 25, 433, 745, 961, 1033, 1138
FatI CATG 6 cut(s) 21, 429, 741, 957, 1029, 1134
FauI CCCGC 2 cut(s) 377, 387
FbaI TGATCA 2 cut(s) 982, 1096
Fnu4HI GCNGC 6 cut(s) 118, 335, 665, 700, 883, 1113
FokI GGATG 1 cut(s) 803
FriOI GRGCYC 2 cut(s) 604, 642
Fsp4HI GCNGC 6 cut(s) 118, 335, 665, 700, 883, 1113
FspBI CTAG 2 cut(s) 114, 1198
GlaI GCGC 3 cut(s) 197, 338, 373
GluI GCNGC 6 cut(s) 118, 335, 665, 700, 883, 1113
GsaI CCCAGC 4 cut(s) 169, 513, 600, 840
GsuI CTGGAG 2 cut(s) 212, 871
HaeII RGCGCY 2 cut(s) 199, 340
HaeIII GGCC 2 cut(s) 26, 157
HapII CCGG 7 cut(s) 11, 395, 463, 468, 761, 798, 829
HgaI GACGC 1 cut(s) 399
HhaI GCGC 3 cut(s) 198, 339, 374
Hin1II CATG 6 cut(s) 25, 433, 745, 961, 1033, 1138
Hin6I GCGC 3 cut(s) 196, 337, 372
HinP1I GCGC 3 cut(s) 196, 337, 372
HincII GTYRAC 1 cut(s) 781
HindII GTYRAC 1 cut(s) 781
HindIII AAGCTT 1 cut(s) 1089
HinfI GANTC 6 cut(s) 53, 77, 607, 723, 755, 801
HpaII CCGG 7 cut(s) 11, 395, 463, 468, 761, 798, 829
HphI GGTGA 3 cut(s) 25, 320, 962
Hpy166II GTNNAC 2 cut(s) 781, 925
Hpy188III TCNNGA 4 cut(s) 323, 631, 958, 980
Hpy8I GTNNAC 2 cut(s) 781, 925
HpyAV CCTTC 2 cut(s) 925, 955
HpyCH4III ACNGT 4 cut(s) 407, 487, 584, 785
HpyCH4V TGCA 1 cut(s) 708
HpyF10VI GCNNNNNNNGC 1 cut(s) 705
HpyF3I CTNAG 4 cut(s) 28, 186, 291, 386
Hsp92II CATG 6 cut(s) 25, 433, 745, 961, 1033, 1138
HspAI GCGC 3 cut(s) 196, 337, 372
Ksp22I TGATCA 2 cut(s) 982, 1096
Kzo9I GATC 7 cut(s) 325, 568, 627, 790, 982, 1079, 1096
LmnI GCTCC 5 cut(s) 193, 425, 607, 890, 1033
Lsp1109I GCAGC 5 cut(s) 129, 346, 676, 686, 1099
LweI GCATC 1 cut(s) 6
MaeI CTAG 2 cut(s) 114, 1198
MaeIII GTNAC 2 cut(s) 226, 1179
MalI GATC 7 cut(s) 327, 570, 629, 792, 984, 1081, 1098
MbiI CCGCTC 1 cut(s) 885
MboI GATC 7 cut(s) 325, 568, 627, 790, 982, 1079, 1096
MfeI CAATTG 1 cut(s) 727
MflI RGATCY 2 cut(s) 568, 627
MhlI GDGCHC 3 cut(s) 44, 604, 642
MluCI AATT 4 cut(s) 233, 727, 1060, 1187
MmeI TCCRAC 2 cut(s) 232, 438
MroXI GAANNNNTTC 1 cut(s) 722
MseI TTAA 2 cut(s) 236, 489
MslI CAYNNNNRTG 3 cut(s) 546, 1107, 1139
MspA1I CMGCKG 2 cut(s) 190, 509
MspI CCGG 7 cut(s) 11, 395, 463, 468, 761, 798, 829
MspR9I CCNGG 2 cut(s) 798, 830
MunI CAATTG 1 cut(s) 727
Mva1269I GAATGC 1 cut(s) 390
MvnI CGCG 1 cut(s) 372
MwoI GCNNNNNNNGC 1 cut(s) 705
NciI CCSGG 2 cut(s) 798, 830
NcoI CCATGG 1 cut(s) 1029
NdeII GATC 7 cut(s) 325, 568, 627, 790, 982, 1079, 1096
NlaIII CATG 6 cut(s) 25, 433, 745, 961, 1033, 1138
NlaIV GGNNCC 3 cut(s) 427, 570, 603
NmuCI GTSAC 2 cut(s) 226, 1179
PagI TCATGA 1 cut(s) 957
PaqCI CACCTGC 1 cut(s) 935
PctI GAATGC 1 cut(s) 390
PdmI GAANNNNTTC 1 cut(s) 722
PfeI GAWTC 6 cut(s) 53, 77, 607, 723, 755, 801
PfoI TCCNGGA 1 cut(s) 796
PinAI ACCGGT 2 cut(s) 10, 467
PkrI GCNGC 6 cut(s) 119, 336, 666, 701, 884, 1114
Psp124BI GAGCTC 1 cut(s) 642
PspFI CCCAGC 4 cut(s) 165, 509, 596, 836
PspN4I GGNNCC 3 cut(s) 427, 570, 603
PspPI GGNCC 3 cut(s) 460, 465, 877
PsuI RGATCY 2 cut(s) 568, 627
PvuII CAGCTG 2 cut(s) 190, 509
RsaI GTAC 7 cut(s) 5, 112, 399, 436, 527, 581, 868
RsaNI GTAC 7 cut(s) 4, 111, 398, 435, 526, 580, 867
RseI CAYNNNNRTG 3 cut(s) 546, 1107, 1139
Rsr2I CGGWCCG 1 cut(s) 465
RsrII CGGWCCG 1 cut(s) 465
SacI GAGCTC 1 cut(s) 642
SaqAI TTAA 2 cut(s) 236, 489
SatI GCNGC 6 cut(s) 118, 335, 665, 700, 883, 1113
Sau3AI GATC 7 cut(s) 325, 568, 627, 790, 982, 1079, 1096
Sau96I GGNCC 3 cut(s) 460, 465, 877
ScaI AGTACT 2 cut(s) 112, 436
ScrFI CCNGG 2 cut(s) 798, 830
SduI GDGCHC 3 cut(s) 44, 604, 642
SfaNI GCATC 1 cut(s) 6
SfcI CTRYAG 1 cut(s) 1162
SinI GGWCC 3 cut(s) 460, 465, 877
SmiMI CAYNNNNRTG 3 cut(s) 546, 1107, 1139
Sse9I AATT 4 cut(s) 233, 727, 1060, 1187
SsiI CCGC 5 cut(s) 266, 370, 380, 880, 883
SspI AATATT 1 cut(s) 695
SspMI CTAG 2 cut(s) 114, 1198
SstI GAGCTC 1 cut(s) 642
StyD4I CCNGG 2 cut(s) 796, 828
StyI CCWWGG 1 cut(s) 1029
TaaI ACNGT 4 cut(s) 407, 487, 584, 785
TaqI TCGA 2 cut(s) 632, 804
TasI AATT 4 cut(s) 233, 727, 1060, 1187
TatI WGTACW 3 cut(s) 3, 110, 434
TauI GCSGC 1 cut(s) 885
TfiI GAWTC 6 cut(s) 53, 77, 607, 723, 755, 801
Tru1I TTAA 2 cut(s) 236, 489
Tru9I TTAA 2 cut(s) 236, 489
TscAI CASTG 1 cut(s) 1162
TseFI GTSAC 2 cut(s) 226, 1179
TseI GCWGC 5 cut(s) 117, 334, 664, 699, 1112
Tsp45I GTSAC 2 cut(s) 226, 1179
TspDTI ATGAA 5 cut(s) 530, 674, 675, 974, 1041
TspGWI ACGGA 1 cut(s) 191
TspRI CASTG 1 cut(s) 1162
VpaK11BI GGWCC 3 cut(s) 460, 465, 877
XcmI CCANNNNNNNNNTGG 1 cut(s) 173
XmnI GAANNNNTTC 1 cut(s) 722
XspI CTAG 2 cut(s) 114, 1198
ZrmI AGTACT 2 cut(s) 112, 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.