Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0013605

Physical Location & Seq

Genomic Coordinates Reverse (-)

30101139 .. 30102845

Gene Structure 1707 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1216800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1119 bp

ATGCTGCTCATGGCCTCTGAGCGGAACACAACAAAACAACCCGCCAAAACCCTTTTCGGGTCCTACGATGAATCCAAATCCGAGCCCGCCAAACCCACCAAAACCACCAAGAAATCCTCCACCAAAGCCTCCTCAAACACTAAAAAGCAACCGCAAAGAGGCCTTGGAGTGGCTCAGCTGGAGCGCCTGAGGCTTCAAGACTGGAAGAAAATGACCGAAGTGCCCCAGCTCCAGCCCCAACCCCAAGTCCTCAATCTTCCTCCTCTTCCTCATCATCATCATCATCAGTACCAAAACCCCATAACACTACCTGACCCGCTTAACAGCGTTCCGGTGCAGTACGGTGCATCCAGGTTCAATGGACCCTCGGTGATGAACGGCTGTGGCGGTTCTGCTGCTACTACAACTTGTTTTTATGGTTTGGTGGGTCAGAGGGTTGGGAATGGTTCTGGGTTTGGTTATGCTGGGAGGAATAATCTGGTGACGAACCTGGATCCGCGTCCGTACGGAGTCTGGGCGCCGGATCCGAGGGTTCAAAGTGGGGCTGTGTACGAGACCTCGAGAGAGCTCTCTTCAATGCCAAAGATACAATCTTTGTCTTCTGATTGCTGTGATCACTGCTTCAAGGGGCTGCAGAAGAAGAGGTTCAATAGTGGGGATTACATGGCTTTGAACGGCAGGAGTGACAAATCTGCGACTGTTTCACCAATCAACGGTTCTGTTTTTCTCGGATTGAACCCTGAAACCGGCAGTTTTGGTGACCGAGTGGGGAGTCGAGCAGCCGGGTACCTGGGGCGCAGCAACCTTGTTGATGATCAGGGTGTAGGGGTTAGGGCAGTGCACAGAAAGGGAGGGTCAGCTGGTGGTGGGATTTTCATGGAATATGAGTTTTTTCCAGCTGCTGATTATCAGAAAAGTGATGAAATTAAAAGAGGATGCACATATCCCAAGCTGCAAACAGTAGGAAGGGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGCTTCTGATATTACTGCTGCTGCAACTTTCAATGGCTATGGGATTGGGGCAGCTGACAGTTACCATGATCATCAACATCATCATCATCCTGTTGATTTGTCACTCAAGCTTTCATTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NOZZLE

Protein Analysis

373

Amino Acids

40.54

Weight (kDa)

8.12

Isoelectric Point (pI)

46.44

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013605)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g05340 FvH4_3g04110
malus_domestica	MD03G1216800.v1.1 MD11G1234600.v1.1
prunus_persica	Prupe.4G192500_v2.0.a1 Prupe.6G133800_v2.0.a1 Prupe.6G133900_v2.0.a1 Prupe.8G142800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0255571
rosa_laevigata	RLG00000014830
rosa_multiflora	Rmu_sc0004935.1_g000041
rosa_roxburghii	Rroxscaffold_7G00209880
rosa_rugosa	Rorug05G0559500
rosa_samantha	Rh6AG078000 Rh6BG070100 Rh6CG068900 Rh6DG065800
rosa_wichuraiana	Rw6G006710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	786
AccB1I	GGYRCC	2 cut(s)	517, 786
AccBSI	CCGCTC	1 cut(s)	22
AccII	CGCG	1 cut(s)	499
AciI	CCGC	7 cut(s)	22, 42, 87, 152, 317, 387, 497
AclWI	GGATC	4 cut(s)	488, 501, 518, 531
AcyI	GRCGYC	1 cut(s)	518
AfaI	GTAC	5 cut(s)	290, 341, 506, 551, 788
AfiI	CCNNNNNNNGG	6 cut(s)	21, 57, 158, 169, 713, 746
AgsI	TTSAA	9 cut(s)	197, 358, 536, 576, 625, 649, 673, 736, 1030
AjnI	CCWGG	3 cut(s)	350, 489, 789
AjuI	GAANNNNNNNTTGG	4 cut(s)	38, 70, 700, 732
AluBI	AGCT	9 cut(s)	178, 229, 568, 860, 899, 952, 1001, 1052, 1108
AluI	AGCT	9 cut(s)	178, 229, 568, 860, 899, 952, 1001, 1052, 1108
Alw21I	GWGCWC	2 cut(s)	570, 843
Alw26I	GTCTC	1 cut(s)	548
Alw44I	GTGCAC	1 cut(s)	839
AlwI	GGATC	4 cut(s)	488, 501, 518, 531
AlwNI	CAGNNNCTG	1 cut(s)	902
Ama87I	CYCGRG	1 cut(s)	559
AoxI	GGCC	2 cut(s)	12, 160
ApaLI	GTGCAC	1 cut(s)	839
Asp700I	GAANNNNTTC	1 cut(s)	644
Asp718I	GGTACC	1 cut(s)	786
AspLEI	GCGC	3 cut(s)	186, 520, 798
AspS9I	GGNCC	2 cut(s)	60, 362
AsuC2I	CCSGG	1 cut(s)	784
AsuHPI	GGTGA	4 cut(s)	382, 493, 696, 770
AvaI	CYCGRG	1 cut(s)	559
AvaII	GGWCC	2 cut(s)	60, 362
AxyI	CCTNAGG	1 cut(s)	188
BaeGI	GKGCMC	2 cut(s)	225, 843
BamHI	GGATCC	2 cut(s)	493, 523
BanI	GGYRCC	2 cut(s)	517, 786
BanII	GRGCYC	2 cut(s)	87, 570
BbsI	GAAGAC	1 cut(s)	591
Bbv12I	GWGCWC	2 cut(s)	570, 843
BbvI	GCAGC	9 cut(s)	382, 618, 791, 810, 886, 939, 1003, 1006, 1061
BceAI	ACGGC	2 cut(s)	394, 691
BciT130I	CCWGG	3 cut(s)	352, 491, 791
BclI	TGATCA	3 cut(s)	613, 814, 1066
BcnI	CCSGG	1 cut(s)	784
BcoDI	GTCTC	1 cut(s)	548
BfmI	CTRYAG	1 cut(s)	632
BfoI	RGCGCY	2 cut(s)	187, 521
BlpI	GCTNAGC	1 cut(s)	174
Bme1390I	CCNGG	4 cut(s)	352, 491, 784, 791
Bme18I	GGWCC	2 cut(s)	60, 362
BmeT110I	CYCGRG	1 cut(s)	559
BmgT120I	GGNCC	2 cut(s)	60, 362
BmiI	GGNNCC	6 cut(s)	61, 364, 495, 519, 525, 788
BmrFI	CCNGG	4 cut(s)	352, 491, 784, 791
BmsI	GCATC	2 cut(s)	356, 926
BpiI	GAAGAC	1 cut(s)	591
BpmI	CTGGAG	2 cut(s)	200, 215
Bpu1102I	GCTNAGC	1 cut(s)	174
BpuEI	CTTGAG	1 cut(s)	1088
BpuMI	CCSGG	1 cut(s)	784
BsaHI	GRCGYC	1 cut(s)	518
BsaI	GGTCTC	1 cut(s)	548
BsaJI	CCNNGG	4 cut(s)	163, 366, 527, 790
BsaWI	WCCGGW	1 cut(s)	331
Bsc4I	CCNNNNNNNGG	6 cut(s)	21, 57, 158, 169, 713, 746
Bse118I	RCCGGY	1 cut(s)	746
Bse1I	ACTGG	1 cut(s)	206
Bse21I	CCTNAGG	1 cut(s)	188
BseBI	CCWGG	3 cut(s)	352, 491, 791
BseDI	CCNNGG	4 cut(s)	163, 366, 527, 790
BseGI	GGATG	3 cut(s)	347, 941, 1084
BseLI	CCNNNNNNNGG	6 cut(s)	21, 57, 158, 169, 713, 746
BseMII	CTCAG	3 cut(s)	9, 179, 188
BseNI	ACTGG	1 cut(s)	206
BseRI	GAGGAG	2 cut(s)	121, 252
BseSI	GKGCMC	2 cut(s)	225, 843
BseXI	GCAGC	9 cut(s)	382, 618, 791, 810, 886, 939, 1003, 1006, 1061
BseYI	CCCAGC	2 cut(s)	225, 464
BsgI	GTGCAG	1 cut(s)	356
Bsh1236I	CGCG	1 cut(s)	499
BshFI	GGCC	2 cut(s)	14, 162
BshNI	GGYRCC	2 cut(s)	517, 786
BsiHKAI	GWGCWC	2 cut(s)	570, 843
BsiHKCI	CYCGRG	1 cut(s)	559
BsiSI	CCGG	4 cut(s)	332, 521, 747, 783
BsiWI	CGTACG	1 cut(s)	504
BslI	CCNNNNNNNGG	6 cut(s)	21, 57, 158, 169, 713, 746
BsmAI	GTCTC	1 cut(s)	548
BsnI	GGCC	2 cut(s)	14, 162
Bso31I	GGTCTC	1 cut(s)	548
BsoBI	CYCGRG	1 cut(s)	559
Bsp1286I	GDGCHC	4 cut(s)	87, 225, 570, 843
Bsp143I	GATC	5 cut(s)	493, 523, 613, 814, 1066
Bsp1720I	GCTNAGC	1 cut(s)	174
BspACI	CCGC	7 cut(s)	22, 42, 87, 152, 317, 387, 497
BspANI	GGCC	2 cut(s)	14, 162
BspCNI	CTCAG	3 cut(s)	10, 180, 187
BspFNI	CGCG	1 cut(s)	499
BspLI	GGNNCC	6 cut(s)	61, 364, 495, 519, 525, 788
BspMAI	CTGCAG	1 cut(s)	636
BspPI	GGATC	4 cut(s)	488, 501, 518, 531
BspT107I	GGYRCC	2 cut(s)	517, 786
BspTNI	GGTCTC	1 cut(s)	548
BsrBI	CCGCTC	1 cut(s)	22
BsrFI	RCCGGY	1 cut(s)	746
BsrI	ACTGG	1 cut(s)	206
BssAI	RCCGGY	1 cut(s)	746
BssECI	CCNNGG	4 cut(s)	163, 366, 527, 790
BssMI	GATC	5 cut(s)	493, 523, 613, 814, 1066
BssNI	GRCGYC	1 cut(s)	518
BssT1I	CCWWGG	1 cut(s)	163
Bst2UI	CCWGG	3 cut(s)	352, 491, 791
Bst4CI	ACNGT	5 cut(s)	344, 700, 716, 961, 1058
Bst6I	CTCTTC	3 cut(s)	270, 577, 635
BstACI	GRCGYC	1 cut(s)	518
BstC8I	GCNNGC	1 cut(s)	87
BstDEI	CTNAG	3 cut(s)	18, 174, 188
BstEII	GGTNACC	1 cut(s)	758
BstF5I	GGATG	3 cut(s)	347, 941, 1084
BstFNI	CGCG	1 cut(s)	499
BstH2I	RGCGCY	2 cut(s)	187, 521
BstHHI	GCGC	3 cut(s)	186, 520, 798
BstKTI	GATC	5 cut(s)	496, 526, 616, 817, 1069
BstMAI	GTCTC	1 cut(s)	548
BstMBI	GATC	5 cut(s)	493, 523, 613, 814, 1066
BstMWI	GCNNNNNNNGC	1 cut(s)	190
BstNI	CCWGG	3 cut(s)	352, 491, 791
BstPI	GGTNACC	1 cut(s)	758
BstSCI	CCNGG	4 cut(s)	350, 489, 782, 789
BstSFI	CTRYAG	1 cut(s)	632
BstSLI	GKGCMC	2 cut(s)	225, 843
BstUI	CGCG	1 cut(s)	499
BstV1I	GCAGC	9 cut(s)	382, 618, 791, 810, 886, 939, 1003, 1006, 1061
BstV2I	GAAGAC	1 cut(s)	591
BstX2I	RGATCY	2 cut(s)	493, 523
BstYI	RGATCY	2 cut(s)	493, 523
Bsu36I	CCTNAGG	1 cut(s)	188
BsuRI	GGCC	2 cut(s)	14, 162
BtsCI	GGATG	3 cut(s)	347, 941, 1084
BtsI	GCAGTG	2 cut(s)	616, 843
BtsIMutI	CAGTG	2 cut(s)	616, 843
Cac8I	GCNNGC	1 cut(s)	87
CaiI	CAGNNNCTG	1 cut(s)	902
CfoI	GCGC	3 cut(s)	186, 520, 798
Cfr10I	RCCGGY	1 cut(s)	746
Cfr13I	GGNCC	2 cut(s)	60, 362
CseI	GACGC	1 cut(s)	488
Csp6I	GTAC	5 cut(s)	289, 340, 505, 550, 787
CviAII	CATG	4 cut(s)	10, 664, 877, 1064
CviQI	GTAC	5 cut(s)	289, 340, 505, 550, 787
DdeI	CTNAG	3 cut(s)	18, 174, 188
DinI	GGCGCC	1 cut(s)	519
DpnI	GATC	5 cut(s)	495, 525, 615, 816, 1068
DpnII	GATC	5 cut(s)	493, 523, 613, 814, 1066
Eam1104I	CTCTTC	3 cut(s)	270, 577, 635
EarI	CTCTTC	3 cut(s)	270, 577, 635
Ecl136II	GAGCTC	1 cut(s)	568
Eco130I	CCWWGG	1 cut(s)	163
Eco147I	AGGCCT	1 cut(s)	162
Eco24I	GRGCYC	2 cut(s)	87, 570
Eco31I	GGTCTC	1 cut(s)	548
Eco47I	GGWCC	2 cut(s)	60, 362
Eco53kI	GAGCTC	1 cut(s)	568
Eco81I	CCTNAGG	1 cut(s)	188
Eco88I	CYCGRG	1 cut(s)	559
Eco91I	GGTNACC	1 cut(s)	758
EcoICRI	GAGCTC	1 cut(s)	568
EcoO109I	RGGNCCY	1 cut(s)	60
EcoO65I	GGTNACC	1 cut(s)	758
EcoRII	CCWGG	3 cut(s)	350, 489, 789
EcoT14I	CCWWGG	1 cut(s)	163
EcoT38I	GRGCYC	2 cut(s)	87, 570
EgeI	GGCGCC	1 cut(s)	519
EheI	GGCGCC	1 cut(s)	519
ErhI	CCWWGG	1 cut(s)	163
FaeI	CATG	4 cut(s)	13, 667, 880, 1067
FatI	CATG	4 cut(s)	9, 663, 876, 1063
FauI	CCCGC	3 cut(s)	49, 94, 324
FbaI	TGATCA	3 cut(s)	613, 814, 1066
FokI	GGATG	3 cut(s)	334, 948, 1071
FriOI	GRGCYC	2 cut(s)	87, 570
GlaI	GCGC	3 cut(s)	185, 519, 797
GsaI	CCCAGC	2 cut(s)	229, 468
GsuI	CTGGAG	2 cut(s)	200, 215
HaeII	RGCGCY	2 cut(s)	187, 521
HaeIII	GGCC	2 cut(s)	14, 162
HapII	CCGG	4 cut(s)	332, 521, 747, 783
HgaI	GACGC	1 cut(s)	488
HhaI	GCGC	3 cut(s)	186, 520, 798
Hin1I	GRCGYC	1 cut(s)	518
Hin1II	CATG	4 cut(s)	13, 667, 880, 1067
Hin6I	GCGC	3 cut(s)	184, 518, 796
HinP1I	GCGC	3 cut(s)	184, 518, 796
HindIII	AAGCTT	2 cut(s)	999, 1106
HinfI	GANTC	3 cut(s)	71, 510, 772
HpaII	CCGG	4 cut(s)	332, 521, 747, 783
HphI	GGTGA	4 cut(s)	382, 493, 696, 770
Hpy166II	GTNNAC	2 cut(s)	550, 841
Hpy188I	TCNGA	8 cut(s)	19, 82, 432, 528, 604, 731, 912, 1006
Hpy188III	TCNNGA	2 cut(s)	197, 561
Hpy8I	GTNNAC	2 cut(s)	550, 841
HpyAV	CCTTC	1 cut(s)	960
HpyCH4III	ACNGT	5 cut(s)	344, 700, 716, 961, 1058
HpyCH4V	TGCA	7 cut(s)	337, 347, 634, 841, 939, 955, 1022
HpyF10VI	GCNNNNNNNGC	1 cut(s)	190
HpyF3I	CTNAG	3 cut(s)	18, 174, 188
Hsp92I	GRCGYC	1 cut(s)	518
Hsp92II	CATG	4 cut(s)	13, 667, 880, 1067
HspAI	GCGC	3 cut(s)	184, 518, 796
KasI	GGCGCC	1 cut(s)	517
KpnI	GGTACC	1 cut(s)	790
Ksp22I	TGATCA	3 cut(s)	613, 814, 1066
Kzo9I	GATC	5 cut(s)	493, 523, 613, 814, 1066
LmnI	GCTCC	2 cut(s)	181, 234
Lsp1109I	GCAGC	9 cut(s)	382, 618, 791, 810, 886, 939, 1003, 1006, 1061
LweI	GCATC	2 cut(s)	356, 926
MaeIII	GTNAC	5 cut(s)	481, 683, 758, 1058, 1098
MalI	GATC	5 cut(s)	495, 525, 615, 816, 1068
MbiI	CCGCTC	1 cut(s)	22
MboI	GATC	5 cut(s)	493, 523, 613, 814, 1066
MflI	RGATCY	2 cut(s)	493, 523
MhlI	GDGCHC	4 cut(s)	87, 225, 570, 843
MluCI	AATT	1 cut(s)	924
Mly113I	GGCGCC	1 cut(s)	518
MlyI	GAGTC	2 cut(s)	519, 781
MroXI	GAANNNNTTC	1 cut(s)	644
MseI	TTAA	2 cut(s)	321, 927
MspA1I	CMGCKG	4 cut(s)	178, 860, 899, 1052
MspI	CCGG	4 cut(s)	332, 521, 747, 783
MspR9I	CCNGG	4 cut(s)	352, 491, 784, 791
MvaI	CCWGG	3 cut(s)	352, 491, 791
MvnI	CGCG	1 cut(s)	499
MwoI	GCNNNNNNNGC	1 cut(s)	190
NarI	GGCGCC	1 cut(s)	518
NciI	CCSGG	1 cut(s)	784
NdeII	GATC	5 cut(s)	493, 523, 613, 814, 1066
NlaIII	CATG	4 cut(s)	13, 667, 880, 1067
NlaIV	GGNNCC	6 cut(s)	61, 364, 495, 519, 525, 788
NmuCI	GTSAC	4 cut(s)	481, 683, 758, 1098
PaeR7I	CTCGAG	1 cut(s)	559
PceI	AGGCCT	1 cut(s)	162
PcsI	WCGNNNNNNNCGW	1 cut(s)	63
PdmI	GAANNNNTTC	1 cut(s)	644
PfeI	GAWTC	1 cut(s)	71
Pfl23II	CGTACG	1 cut(s)	504
PleI	GAGTC	2 cut(s)	518, 780
PluTI	GGCGCC	1 cut(s)	521
PpsI	GAGTC	2 cut(s)	518, 780
PpuMI	RGGWCCY	1 cut(s)	60
Psp124BI	GAGCTC	1 cut(s)	570
Psp5II	RGGWCCY	1 cut(s)	60
Psp6I	CCWGG	3 cut(s)	350, 489, 789
PspEI	GGTNACC	1 cut(s)	758
PspFI	CCCAGC	2 cut(s)	225, 464
PspGI	CCWGG	3 cut(s)	350, 489, 789
PspLI	CGTACG	1 cut(s)	504
PspN4I	GGNNCC	6 cut(s)	61, 364, 495, 519, 525, 788
PspPI	GGNCC	2 cut(s)	60, 362
PspPPI	RGGWCCY	1 cut(s)	60
PstI	CTGCAG	1 cut(s)	636
PstNI	CAGNNNCTG	1 cut(s)	902
PsuI	RGATCY	2 cut(s)	493, 523
PvuII	CAGCTG	4 cut(s)	178, 860, 899, 1052
RsaI	GTAC	5 cut(s)	290, 341, 506, 551, 788
RsaNI	GTAC	5 cut(s)	289, 340, 505, 550, 787
SacI	GAGCTC	1 cut(s)	570
SaqAI	TTAA	2 cut(s)	321, 927
Sau3AI	GATC	5 cut(s)	493, 523, 613, 814, 1066
Sau96I	GGNCC	2 cut(s)	60, 362
SchI	GAGTC	2 cut(s)	519, 781
ScrFI	CCNGG	4 cut(s)	352, 491, 784, 791
SduI	GDGCHC	4 cut(s)	87, 225, 570, 843
SfaNI	GCATC	2 cut(s)	356, 926
SfcI	CTRYAG	1 cut(s)	632
SfoI	GGCGCC	1 cut(s)	519
Sfr274I	CTCGAG	1 cut(s)	559
SinI	GGWCC	2 cut(s)	60, 362
SlaI	CTCGAG	1 cut(s)	559
SmlI	CTYRAG	2 cut(s)	559, 1103
SmoI	CTYRAG	2 cut(s)	559, 1103
Sse9I	AATT	1 cut(s)	924
SseBI	AGGCCT	1 cut(s)	162
SsiI	CCGC	7 cut(s)	22, 42, 87, 152, 317, 387, 497
SspDI	GGCGCC	1 cut(s)	517
SstI	GAGCTC	1 cut(s)	570
StuI	AGGCCT	1 cut(s)	162
StyD4I	CCNGG	4 cut(s)	350, 489, 782, 789
StyI	CCWWGG	1 cut(s)	163
TaaI	ACNGT	5 cut(s)	344, 700, 716, 961, 1058
TaqI	TCGA	2 cut(s)	560, 775
TaqII	GACCGA	2 cut(s)	230, 777
TasI	AATT	1 cut(s)	924
TfiI	GAWTC	1 cut(s)	71
Tru1I	TTAA	2 cut(s)	321, 927
Tru9I	TTAA	2 cut(s)	321, 927
TscAI	CASTG	2 cut(s)	623, 843
TseFI	GTSAC	4 cut(s)	481, 683, 758, 1098
Tsp45I	GTSAC	4 cut(s)	481, 683, 758, 1098
TspDTI	ATGAA	5 cut(s)	84, 389, 865, 936, 1101
TspGWI	ACGGA	2 cut(s)	492, 522
TspRI	CASTG	2 cut(s)	623, 843
VneI	GTGCAC	1 cut(s)	839
VpaK11BI	GGWCC	2 cut(s)	60, 362
XhoI	CTCGAG	1 cut(s)	559
XmnI	GAANNNNTTC	1 cut(s)	644

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.