RLG00000014830

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0013605
Physical Location & Seq
Reverse (-)
58159994 .. 58161488
1495 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014830

Sequence Viewer

Length: 1188 bp
ATGTACAGACCGGTGATGCTCATGGCCTCAGATTACCAGAGCACAACCCAAGATTCTACCCGTTTTGGGTGCGAAGAATCCAAAGAACCCGAAACCACCACCAAAACCAGTGCTAGCAGCAAAGGAAAATCAAGGAAGTCAGCTAAACCTAAAAGACCAAAACCCCAGAGAGGTGTTGGTGTGGCTCAGCTGGAGCGCCTCCGTCTCCAACAGAGAGGTGTAGGTGTGACCAAATTAAACCAAATGCCCCTCCCCCATATCCTACCCCAACCTCAACCTCAGACCCAAGTTGTCCAAAATGTTGGTTTTCCAGATCACCAGCAGCGCCAGTTTCAATACTACCCGAACCCAGAATCCCGCGCCGACCCGCTTCTCAGCATTCCGGTACTCTATGGTGCGTCCAACTACGATGGAGCCATGAGTACTGGCTCTGGTTTGGTGGGCTTGGACCGGACCGGTTTGTTGGTTCAGACAGTTAATGGGAATGGTGGTTCAGCTGGGTTTGGTTATGGGTACAATGTTCGTCTTCATAATGATGTTGGTGGGTCGGTTATGGATCCGAACCAGTACGGTGTCGTTGGGGCTGGGGCTCAGAATCGTGGGGCTGTGTATGAGATCTCGAACGAGCTCTCTTCAATACCAAAGATGAAGCAGCAACCCTCCTCTTCTTCTTCATCTGAATATTGCTGCTGTTGCAAGCAGAAGAAAAGATTCAATTGTGGAAACAACATGGGTTTTGGGGAATCTCCGGCTGTCTTTCCGATAGTCAACAGTTCTGATCTTCCCGGATTCGACCAACAAAACATCCGAAATGTCCGGCTAGCTGGGGAAATCGGCGTTGGTTTCAATGCTGGTACTTACTTGGGCCGCCGCTCCAGTCTTGTTGATGAGAGTGTGGAGGTTAGGGCAGTTCATAGGAAGGGGAGACAAGCAGGTGGTGATATCATGAAGGAGTATGAATTCTTTCCTGATCACAACAACAACTCTTCTTCAGTAGTCTCATCAGGCACATATCCCATGGAGCATGATATAGTTGCTTCATCAACTAATTCTTCTTCAGTTGGTAGATCATCAGAAGCTTCTGATCAACATCTTGGTGCTGCTTGTATTGAGGGAGCTGACATGATGATGATGATGATGAGCAGTGGTTATAGTCCTGTTGATTTGTCACTCAAATTGTCTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NOZZLE

Protein Analysis

396

Amino Acids

42.67

Weight (kDa)

8.57

Isoelectric Point (pI)

48.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 923
Acc36I ACCTGC 1 cut(s) 923
AccBSI CCGCTC 1 cut(s) 873
AccII CGCG 1 cut(s) 360
AciI CCGC 4 cut(s) 358, 368, 868, 871
AclWI GGATC 2 cut(s) 551, 564
AcsI RAATTY 1 cut(s) 959
AcuI CTGAAG 2 cut(s) 975, 1041
AfaI GTAC 6 cut(s) 5, 387, 424, 515, 569, 856
AfiI CCNNNNNNNGG 2 cut(s) 66, 170
AgeI ACCGGT 2 cut(s) 10, 455
AgsI TTSAA 4 cut(s) 335, 636, 715, 847
AjuI GAANNNNNNNTTGG 2 cut(s) 822, 854
AloI GAACNNNNNNTCC 4 cut(s) 338, 370, 475, 507
AluBI AGCT 7 cut(s) 143, 190, 497, 628, 824, 1079, 1118
AluI AGCT 7 cut(s) 143, 190, 497, 628, 824, 1079, 1118
Alw21I GWGCWC 2 cut(s) 44, 630
Alw26I GTCTC 3 cut(s) 209, 919, 1003
AlwI GGATC 2 cut(s) 551, 564
AoxI GGCC 2 cut(s) 24, 865
ApeKI GCWGC 5 cut(s) 117, 322, 652, 687, 1100
ApoI RAATTY 1 cut(s) 959
AsiGI ACCGGT 2 cut(s) 10, 455
Asp700I GAANNNNTTC 2 cut(s) 710, 963
AspLEI GCGC 3 cut(s) 198, 327, 362
AspS9I GGNCC 3 cut(s) 448, 453, 865
AsuC2I CCSGG 1 cut(s) 786
AsuHPI GGTGA 3 cut(s) 25, 308, 950
AsuNHI GCTAGC 2 cut(s) 113, 820
AvaII GGWCC 2 cut(s) 448, 453
BamHI GGATCC 1 cut(s) 556
BanII GRGCYC 2 cut(s) 592, 630
BbsI GAAGAC 1 cut(s) 518
Bbv12I GWGCWC 2 cut(s) 44, 630
BbvI GCAGC 5 cut(s) 129, 334, 664, 674, 1087
BccI CCATC 1 cut(s) 404
BclI TGATCA 2 cut(s) 970, 1084
BcnI CCSGG 1 cut(s) 786
BcoDI GTCTC 3 cut(s) 209, 919, 1003
BfaI CTAG 3 cut(s) 114, 821, 1186
BfoI RGCGCY 2 cut(s) 199, 328
BfuAI ACCTGC 1 cut(s) 923
BglII AGATCT 1 cut(s) 615
BisI GCNGC 7 cut(s) 118, 323, 653, 688, 868, 871, 1101
BlpI GCTNAGC 1 cut(s) 186
BlsI GCNGC 7 cut(s) 119, 324, 654, 689, 869, 872, 1102
BmcAI AGTACT 1 cut(s) 424
Bme1390I CCNGG 1 cut(s) 786
Bme18I GGWCC 2 cut(s) 448, 453
BmgT120I GGNCC 3 cut(s) 448, 453, 865
BmiI GGNNCC 2 cut(s) 415, 558
BmrFI CCNGG 1 cut(s) 786
BmsI GCATC 1 cut(s) 6
BmtI GCTAGC 2 cut(s) 117, 824
BpiI GAAGAC 1 cut(s) 518
BpmI CTGGAG 2 cut(s) 212, 859
Bpu1102I GCTNAGC 1 cut(s) 186
BpuMI CCSGG 1 cut(s) 786
BsaBI GATNNNNATC 1 cut(s) 1089
BsaJI CCNNGG 1 cut(s) 1017
BsaWI WCCGGW 4 cut(s) 10, 382, 450, 455
Bsc4I CCNNNNNNNGG 2 cut(s) 66, 170
Bse118I RCCGGY 2 cut(s) 10, 455
Bse1I ACTGG 5 cut(s) 108, 328, 430, 565, 876
Bse8I GATNNNNATC 1 cut(s) 1089
BseDI CCNNGG 1 cut(s) 1017
BseGI GGATG 1 cut(s) 804
BseJI GATNNNNATC 1 cut(s) 1089
BseLI CCNNNNNNNGG 2 cut(s) 66, 170
BseMII CTCAG 5 cut(s) 42, 200, 293, 388, 605
BseNI ACTGG 5 cut(s) 108, 328, 430, 565, 876
BseRI GAGGAG 1 cut(s) 652
BseXI GCAGC 5 cut(s) 129, 334, 664, 674, 1087
BseYI CCCAGC 3 cut(s) 497, 584, 824
Bsh1236I CGCG 1 cut(s) 360
BshFI GGCC 2 cut(s) 26, 867
BshTI ACCGGT 2 cut(s) 10, 455
BsiHKAI GWGCWC 2 cut(s) 44, 630
BsiSI CCGG 7 cut(s) 11, 383, 451, 456, 749, 786, 817
BslI CCNNNNNNNGG 2 cut(s) 66, 170
BsmAI GTCTC 3 cut(s) 209, 919, 1003
BsmBI CGTCTC 1 cut(s) 209
BsmI GAATGC 1 cut(s) 378
BsnI GGCC 2 cut(s) 26, 867
Bsp1286I GDGCHC 3 cut(s) 44, 592, 630
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 7 cut(s) 313, 556, 615, 778, 970, 1067, 1084
Bsp1720I GCTNAGC 1 cut(s) 186
Bsp19I CCATGG 1 cut(s) 1017
BspACI CCGC 4 cut(s) 358, 368, 868, 871
BspANI GGCC 2 cut(s) 26, 867
BspCNI CTCAG 5 cut(s) 41, 199, 292, 387, 604
BspFNI CGCG 1 cut(s) 360
BspHI TCATGA 1 cut(s) 945
BspLI GGNNCC 2 cut(s) 415, 558
BspMI ACCTGC 1 cut(s) 923
BspOI GCTAGC 2 cut(s) 117, 824
BspPI GGATC 2 cut(s) 551, 564
BsrBI CCGCTC 1 cut(s) 873
BsrFI RCCGGY 2 cut(s) 10, 455
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 5 cut(s) 108, 328, 430, 565, 876
BssAI RCCGGY 2 cut(s) 10, 455
BssECI CCNNGG 1 cut(s) 1017
BssMI GATC 7 cut(s) 313, 556, 615, 778, 970, 1067, 1084
BssT1I CCWWGG 1 cut(s) 1017
Bst4CI ACNGT 3 cut(s) 475, 572, 773
Bst6I CTCTTC 3 cut(s) 637, 670, 991
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 3 cut(s) 115, 698, 822
BstDEI CTNAG 5 cut(s) 28, 186, 279, 374, 591
BstDSI CCRYGG 1 cut(s) 1017
BstF5I GGATG 1 cut(s) 804
BstFNI CGCG 1 cut(s) 360
BstH2I RGCGCY 2 cut(s) 199, 328
BstHHI GCGC 3 cut(s) 198, 327, 362
BstKTI GATC 7 cut(s) 316, 559, 618, 781, 973, 1070, 1087
BstMAI GTCTC 3 cut(s) 209, 919, 1003
BstMBI GATC 7 cut(s) 313, 556, 615, 778, 970, 1067, 1084
BstMWI GCNNNNNNNGC 1 cut(s) 693
BstSCI CCNGG 1 cut(s) 784
BstUI CGCG 1 cut(s) 360
BstV1I GCAGC 5 cut(s) 129, 334, 664, 674, 1087
BstV2I GAAGAC 1 cut(s) 518
BstX2I RGATCY 2 cut(s) 556, 615
BstXI CCANNNNNNTGG 1 cut(s) 302
BstYI RGATCY 2 cut(s) 556, 615
BsuRI GGCC 2 cut(s) 26, 867
BtgI CCRYGG 1 cut(s) 1017
BtsCI GGATG 1 cut(s) 804
BtsI GCAGTG 1 cut(s) 1150
BtsIMutI CAGTG 2 cut(s) 115, 1150
BveI ACCTGC 1 cut(s) 923
Cac8I GCNNGC 3 cut(s) 115, 698, 822
CciI TCATGA 1 cut(s) 945
CfoI GCGC 3 cut(s) 198, 327, 362
Cfr10I RCCGGY 2 cut(s) 10, 455
Cfr13I GGNCC 3 cut(s) 448, 453, 865
CpoI CGGWCCG 1 cut(s) 453
CseI GACGC 1 cut(s) 387
Csp6I GTAC 6 cut(s) 4, 386, 423, 514, 568, 855
CspAI ACCGGT 2 cut(s) 10, 455
CspI CGGWCCG 1 cut(s) 453
CviAII CATG 7 cut(s) 22, 418, 730, 946, 1018, 1025, 1123
CviQI GTAC 6 cut(s) 4, 386, 423, 514, 568, 855
DdeI CTNAG 5 cut(s) 28, 186, 279, 374, 591
DpnI GATC 7 cut(s) 315, 558, 617, 780, 972, 1069, 1086
DpnII GATC 7 cut(s) 313, 556, 615, 778, 970, 1067, 1084
Eam1104I CTCTTC 3 cut(s) 637, 670, 991
EarI CTCTTC 3 cut(s) 637, 670, 991
Ecl136II GAGCTC 1 cut(s) 628
Eco130I CCWWGG 1 cut(s) 1017
Eco24I GRGCYC 2 cut(s) 592, 630
Eco32I GATATC 1 cut(s) 943
Eco47I GGWCC 2 cut(s) 448, 453
Eco53kI GAGCTC 1 cut(s) 628
Eco57I CTGAAG 2 cut(s) 975, 1041
EcoICRI GAGCTC 1 cut(s) 628
EcoRI GAATTC 1 cut(s) 959
EcoRV GATATC 1 cut(s) 943
EcoT14I CCWWGG 1 cut(s) 1017
EcoT38I GRGCYC 2 cut(s) 592, 630
ErhI CCWWGG 1 cut(s) 1017
Esp3I CGTCTC 1 cut(s) 209
FaeI CATG 7 cut(s) 25, 421, 733, 949, 1021, 1028, 1126
FatI CATG 7 cut(s) 21, 417, 729, 945, 1017, 1024, 1122
FauI CCCGC 2 cut(s) 365, 375
FbaI TGATCA 2 cut(s) 970, 1084
Fnu4HI GCNGC 7 cut(s) 118, 323, 653, 688, 868, 871, 1101
FokI GGATG 1 cut(s) 791
FriOI GRGCYC 2 cut(s) 592, 630
Fsp4HI GCNGC 7 cut(s) 118, 323, 653, 688, 868, 871, 1101
FspBI CTAG 3 cut(s) 114, 821, 1186
GlaI GCGC 3 cut(s) 197, 326, 361
GluI GCNGC 7 cut(s) 118, 323, 653, 688, 868, 871, 1101
GsaI CCCAGC 3 cut(s) 501, 588, 828
GsuI CTGGAG 2 cut(s) 212, 859
HaeII RGCGCY 2 cut(s) 199, 328
HaeIII GGCC 2 cut(s) 26, 867
HapII CCGG 7 cut(s) 11, 383, 451, 456, 749, 786, 817
HgaI GACGC 1 cut(s) 387
HhaI GCGC 3 cut(s) 198, 327, 362
Hin1II CATG 7 cut(s) 25, 421, 733, 949, 1021, 1028, 1126
Hin6I GCGC 3 cut(s) 196, 325, 360
HinP1I GCGC 3 cut(s) 196, 325, 360
HincII GTYRAC 1 cut(s) 769
HindII GTYRAC 1 cut(s) 769
HindIII AAGCTT 1 cut(s) 1077
HinfI GANTC 7 cut(s) 53, 77, 353, 595, 711, 743, 789
HpaII CCGG 7 cut(s) 11, 383, 451, 456, 749, 786, 817
HphI GGTGA 3 cut(s) 25, 308, 950
Hpy166II GTNNAC 1 cut(s) 769
Hpy188III TCNNGA 4 cut(s) 311, 619, 946, 968
Hpy8I GTNNAC 1 cut(s) 769
HpyAV CCTTC 2 cut(s) 913, 943
HpyCH4III ACNGT 3 cut(s) 475, 572, 773
HpyCH4V TGCA 1 cut(s) 696
HpyF10VI GCNNNNNNNGC 1 cut(s) 693
HpyF3I CTNAG 5 cut(s) 28, 186, 279, 374, 591
Hsp92II CATG 7 cut(s) 25, 421, 733, 949, 1021, 1028, 1126
HspAI GCGC 3 cut(s) 196, 325, 360
Ksp22I TGATCA 2 cut(s) 970, 1084
Kzo9I GATC 7 cut(s) 313, 556, 615, 778, 970, 1067, 1084
LmnI GCTCC 5 cut(s) 193, 413, 878, 1021, 1115
Lsp1109I GCAGC 5 cut(s) 129, 334, 664, 674, 1087
LweI GCATC 1 cut(s) 6
MaeI CTAG 3 cut(s) 114, 821, 1186
MaeIII GTNAC 2 cut(s) 226, 1167
MalI GATC 7 cut(s) 315, 558, 617, 780, 972, 1069, 1086
MbiI CCGCTC 1 cut(s) 873
MboI GATC 7 cut(s) 313, 556, 615, 778, 970, 1067, 1084
MfeI CAATTG 1 cut(s) 715
MflI RGATCY 2 cut(s) 556, 615
MhlI GDGCHC 3 cut(s) 44, 592, 630
MluCI AATT 5 cut(s) 233, 715, 959, 1048, 1175
MmeI TCCRAC 2 cut(s) 232, 426
MroXI GAANNNNTTC 2 cut(s) 710, 963
MseI TTAA 2 cut(s) 236, 477
MslI CAYNNNNRTG 3 cut(s) 534, 1095, 1127
MspA1I CMGCKG 2 cut(s) 190, 497
MspI CCGG 7 cut(s) 11, 383, 451, 456, 749, 786, 817
MspR9I CCNGG 1 cut(s) 786
MunI CAATTG 1 cut(s) 715
Mva1269I GAATGC 1 cut(s) 378
MvnI CGCG 1 cut(s) 360
MwoI GCNNNNNNNGC 1 cut(s) 693
NciI CCSGG 1 cut(s) 786
NcoI CCATGG 1 cut(s) 1017
NdeII GATC 7 cut(s) 313, 556, 615, 778, 970, 1067, 1084
NheI GCTAGC 2 cut(s) 113, 820
NlaIII CATG 7 cut(s) 25, 421, 733, 949, 1021, 1028, 1126
NlaIV GGNNCC 2 cut(s) 415, 558
NmuCI GTSAC 2 cut(s) 226, 1167
PagI TCATGA 1 cut(s) 945
PaqCI CACCTGC 1 cut(s) 923
PctI GAATGC 1 cut(s) 378
PdmI GAANNNNTTC 2 cut(s) 710, 963
PfeI GAWTC 7 cut(s) 53, 77, 353, 595, 711, 743, 789
PfoI TCCNGGA 1 cut(s) 784
PinAI ACCGGT 2 cut(s) 10, 455
PkrI GCNGC 7 cut(s) 119, 324, 654, 689, 869, 872, 1102
Psp124BI GAGCTC 1 cut(s) 630
PspFI CCCAGC 3 cut(s) 497, 584, 824
PspN4I GGNNCC 2 cut(s) 415, 558
PspPI GGNCC 3 cut(s) 448, 453, 865
PsuI RGATCY 2 cut(s) 556, 615
PvuII CAGCTG 2 cut(s) 190, 497
RsaI GTAC 6 cut(s) 5, 387, 424, 515, 569, 856
RsaNI GTAC 6 cut(s) 4, 386, 423, 514, 568, 855
RseI CAYNNNNRTG 3 cut(s) 534, 1095, 1127
Rsr2I CGGWCCG 1 cut(s) 453
RsrII CGGWCCG 1 cut(s) 453
SacI GAGCTC 1 cut(s) 630
SaqAI TTAA 2 cut(s) 236, 477
SatI GCNGC 7 cut(s) 118, 323, 653, 688, 868, 871, 1101
Sau3AI GATC 7 cut(s) 313, 556, 615, 778, 970, 1067, 1084
Sau96I GGNCC 3 cut(s) 448, 453, 865
ScaI AGTACT 1 cut(s) 424
ScrFI CCNGG 1 cut(s) 786
SduI GDGCHC 3 cut(s) 44, 592, 630
SfaNI GCATC 1 cut(s) 6
SinI GGWCC 2 cut(s) 448, 453
SmiMI CAYNNNNRTG 3 cut(s) 534, 1095, 1127
Sse9I AATT 5 cut(s) 233, 715, 959, 1048, 1175
SsiI CCGC 4 cut(s) 358, 368, 868, 871
SspI AATATT 1 cut(s) 683
SspMI CTAG 3 cut(s) 114, 821, 1186
SstI GAGCTC 1 cut(s) 630
StyD4I CCNGG 1 cut(s) 784
StyI CCWWGG 1 cut(s) 1017
TaaI ACNGT 3 cut(s) 475, 572, 773
TaqI TCGA 2 cut(s) 620, 792
TasI AATT 5 cut(s) 233, 715, 959, 1048, 1175
TatI WGTACW 2 cut(s) 3, 422
TauI GCSGC 2 cut(s) 870, 873
TfiI GAWTC 7 cut(s) 53, 77, 353, 595, 711, 743, 789
Tru1I TTAA 2 cut(s) 236, 477
Tru9I TTAA 2 cut(s) 236, 477
TscAI CASTG 2 cut(s) 115, 1150
TseFI GTSAC 2 cut(s) 226, 1167
TseI GCWGC 5 cut(s) 117, 322, 652, 687, 1100
Tsp45I GTSAC 2 cut(s) 226, 1167
TspDTI ATGAA 7 cut(s) 518, 662, 663, 902, 962, 972, 1029
TspGWI ACGGA 1 cut(s) 191
TspRI CASTG 2 cut(s) 115, 1150
VpaK11BI GGWCC 2 cut(s) 448, 453
XapI RAATTY 1 cut(s) 959
XcmI CCANNNNNNNNNTGG 1 cut(s) 173
XmnI GAANNNNTTC 2 cut(s) 710, 963
XspI CTAG 3 cut(s) 114, 821, 1186
ZrmI AGTACT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.