FvH4_2g05480

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011916
Physical Location & Seq
Reverse (-)
4518187 .. 4521508
3322 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g05480.t1

Sequence Viewer

Length: 939 bp
ATGTCCTATTATCAATTTCCAACGCAGTCTTCTTCGTCTTCGTCCCCTTACTCATTGTTTCCCATGTCAATTTCTGGCATTCAAGGCCAGCTTCTTGAGGTCACCATCGTTGGGTGTAACAAGTTGAAAGATACTGAATGGATTTCAAGGCAAGATCCTTACGTTTGTATTGAATATGCTAACTCCAAGTTCCGAACAAGAACGCATACTGAATCGAATTTGGACTCGAACTGGACTGAAACTCATGTTGATTTAATTGGTGTAGATGGAGGTAAAAACCCCACATTCCAAGAGAAATTCGTGATTCCTTTGATTGAAGGGCTTAGGGTGATCAATGTTAATGTCTGGAACAGCAACAGTGTCACATATGATGACTTTATTGGCGAGGGAAAAATTCAACTTCAGAAGGTTCTGGATCAGGGTTATGATGATAGTACTTGGCCGCTTCAGACTAAACATGGCAGATATGCAGGAGAAGTGCGGCTTATAATGCATTACACAAAGCCTAACGCAAGTGCAAAGCCTAATAAACCGGCAGCAGGCTATGCCCCATCTGCACCACCATATGGAGCACCACCTGCACCACAAGCTCATTTGTACTCAACTCCCCCTCCAGCATCTGCATATGCACCACCACCTCAAATGTATGCACAACCTCCACCAGCAGCCTACCCCGCTCCATCTCCATACCCTTCGTATTCATCTAATCCTTCATATCCACCCAATCCAGCTGGGTATCCACCTAATCCAGCACCAGGAGGCTATCCACCAAACCCGTACGGTGCCCCTCCACAAGCTGCATATCCTCCTGCCCCATACCCTCCATCTTCAACATATCCTCCCCCTCCATCATCTACATATCCACCACCTCCACAAGCCTCATCCTTTTACCCTCCACCTCAAGGTCCATATGGAATTTATCCTCCACCACCATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04620 map04620
Pfam Domains
Protein Families
C2

Protein Analysis

313

Amino Acids

33.94

Weight (kDa)

6.19

Isoelectric Point (pI)

74.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 92 - 147 7.4e-07 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011916)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34150
fragaria_vesca FvH4_2g05480
malus_domestica MD00G1045300.v1.1 MD05G1087200.v1.1
prunus_persica Prupe.8G131800_v2.0.a1 Prupe.8G131800_v2.0.a1
pyrus_communis pycom05g08650 pycom10g08200
rosa_chinensis RchiOBHm_Chr6g0255751
rosa_laevigata RLG00000014816
rosa_multiflora Rmu_sc0001163.1_g000008 Rmu_sc0001163.1_g000009
rosa_roxburghii Rroxscaffold_7G00209700
rosa_rugosa Rorug05G0561000 Rorug05G0561100
rosa_samantha Rh6BG072200 Rh6CG070400 Rh6DG067600
rosa_wichuraiana Rw6G006820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 488
AarI CACCTGC 1 cut(s) 586
Acc36I ACCTGC 1 cut(s) 586
AccB1I GGYRCC 1 cut(s) 782
AccB7I CCANNNNNTGG 1 cut(s) 566
AccBSI CCGCTC 1 cut(s) 677
AciI CCGC 3 cut(s) 443, 481, 675
AclWI GGATC 2 cut(s) 149, 423
AcoI YGGCCR 1 cut(s) 440
AcsI RAATTY 4 cut(s) 217, 296, 393, 915
AcuI CTGAAG 2 cut(s) 386, 431
AfaI GTAC 3 cut(s) 436, 599, 779
AfiI CCNNNNNNNGG 5 cut(s) 111, 539, 566, 755, 902
AgsI TTSAA 7 cut(s) 83, 127, 147, 173, 317, 398, 831
AjnI CCWGG 1 cut(s) 754
AjuI GAANNNNNNNTTGG 2 cut(s) 13, 45
AluBI AGCT 4 cut(s) 91, 590, 731, 797
AluI AGCT 4 cut(s) 91, 590, 731, 797
Alw21I GWGCWC 1 cut(s) 574
AlwI GGATC 2 cut(s) 149, 423
AlwNI CAGNNNCTG 1 cut(s) 620
AoxI GGCC 2 cut(s) 85, 440
ApeKI GCWGC 3 cut(s) 536, 665, 797
ApoI RAATTY 4 cut(s) 217, 296, 393, 915
AspS9I GGNCC 1 cut(s) 905
AsuHPI GGTGA 2 cut(s) 94, 340
AvaII GGWCC 1 cut(s) 905
BaeGI GKGCMC 1 cut(s) 787
BanI GGYRCC 1 cut(s) 782
BbsI GAAGAC 2 cut(s) 21, 30
Bbv12I GWGCWC 1 cut(s) 574
BbvI GCAGC 3 cut(s) 548, 677, 784
BccI CCATC 6 cut(s) 113, 260, 559, 688, 832, 856
BciT130I CCWGG 1 cut(s) 756
BciVI GTATCC 1 cut(s) 747
BclI TGATCA 1 cut(s) 330
BfuAI ACCTGC 1 cut(s) 586
BfuI GTATCC 1 cut(s) 747
BisI GCNGC 5 cut(s) 443, 482, 537, 666, 798
BlsI GCNGC 5 cut(s) 444, 483, 538, 667, 799
BmcAI AGTACT 1 cut(s) 436
Bme1390I CCNGG 1 cut(s) 756
Bme18I GGWCC 1 cut(s) 905
BmgT120I GGNCC 1 cut(s) 905
BmiI GGNNCC 1 cut(s) 784
BmrFI CCNGG 1 cut(s) 756
BmsI GCATC 1 cut(s) 626
BpiI GAAGAC 2 cut(s) 21, 30
BpmI CTGGAG 1 cut(s) 597
Bpu10I CCTNAGC 1 cut(s) 323
BpuEI CTTGAG 2 cut(s) 116, 885
BsaXI ACNNNNNCTCC 4 cut(s) 595, 625, 823, 853
Bsc4I CCNNNNNNNGG 5 cut(s) 111, 539, 566, 755, 902
Bse118I RCCGGY 1 cut(s) 532
Bse1I ACTGG 1 cut(s) 236
BseBI CCWGG 1 cut(s) 756
BseGI GGATG 1 cut(s) 881
BseLI CCNNNNNNNGG 5 cut(s) 111, 539, 566, 755, 902
BseNI ACTGG 1 cut(s) 236
BseSI GKGCMC 1 cut(s) 787
BseXI GCAGC 3 cut(s) 548, 677, 784
BseYI CCCAGC 1 cut(s) 731
BsgI GTGCAG 2 cut(s) 540, 564
BshFI GGCC 2 cut(s) 87, 442
BshNI GGYRCC 1 cut(s) 782
BsiHKAI GWGCWC 1 cut(s) 574
BsiSI CCGG 1 cut(s) 533
BsiWI CGTACG 1 cut(s) 777
BslFI GGGAC 1 cut(s) 28
BslI CCNNNNNNNGG 5 cut(s) 111, 539, 566, 755, 902
BsmFI GGGAC 1 cut(s) 28
BsmI GAATGC 1 cut(s) 78
BsnI GGCC 2 cut(s) 87, 442
Bsp1286I GDGCHC 2 cut(s) 574, 787
Bsp143I GATC 3 cut(s) 154, 330, 415
BspACI CCGC 3 cut(s) 443, 481, 675
BspANI GGCC 2 cut(s) 87, 442
BspLI GGNNCC 1 cut(s) 784
BspMI ACCTGC 1 cut(s) 586
BspPI GGATC 2 cut(s) 149, 423
BspT107I GGYRCC 1 cut(s) 782
BsrBI CCGCTC 1 cut(s) 677
BsrFI RCCGGY 1 cut(s) 532
BsrI ACTGG 1 cut(s) 236
BssAI RCCGGY 1 cut(s) 532
BssMI GATC 3 cut(s) 154, 330, 415
Bst2UI CCWGG 1 cut(s) 756
Bst4CI ACNGT 2 cut(s) 359, 782
BstAPI GCANNNNNTGC 2 cut(s) 545, 578
BstC8I GCNNGC 2 cut(s) 89, 541
BstDEI CTNAG 1 cut(s) 323
BstEII GGTNACC 1 cut(s) 100
BstF5I GGATG 1 cut(s) 881
BstKTI GATC 3 cut(s) 157, 333, 418
BstMBI GATC 3 cut(s) 154, 330, 415
BstMWI GCNNNNNNNGC 7 cut(s) 84, 490, 545, 554, 578, 587, 674
BstNI CCWGG 1 cut(s) 756
BstPI GGTNACC 1 cut(s) 100
BstSCI CCNGG 1 cut(s) 754
BstSLI GKGCMC 1 cut(s) 787
BstV1I GCAGC 3 cut(s) 548, 677, 784
BstV2I GAAGAC 2 cut(s) 21, 30
BstX2I RGATCY 1 cut(s) 154
BstYI RGATCY 1 cut(s) 154
BsuI GTATCC 1 cut(s) 747
BsuRI GGCC 2 cut(s) 87, 442
BtsCI GGATG 1 cut(s) 881
BtsIMutI CAGTG 1 cut(s) 364
BveI ACCTGC 1 cut(s) 586
Cac8I GCNNGC 2 cut(s) 89, 541
CaiI CAGNNNCTG 1 cut(s) 620
Cfr10I RCCGGY 1 cut(s) 532
Cfr13I GGNCC 1 cut(s) 905
Csp6I GTAC 3 cut(s) 435, 598, 778
CviAII CATG 3 cut(s) 64, 245, 458
CviQI GTAC 3 cut(s) 435, 598, 778
DdeI CTNAG 1 cut(s) 323
DpnI GATC 3 cut(s) 156, 332, 417
DpnII GATC 3 cut(s) 154, 330, 415
EaeI YGGCCR 1 cut(s) 440
Eco47I GGWCC 1 cut(s) 905
Eco57I CTGAAG 2 cut(s) 386, 431
Eco91I GGTNACC 1 cut(s) 100
EcoO65I GGTNACC 1 cut(s) 100
EcoRII CCWGG 1 cut(s) 754
EcoT22I ATGCAT 1 cut(s) 495
FaeI CATG 3 cut(s) 67, 248, 461
FalI AAGNNNNNCTT 4 cut(s) 75, 107, 468, 500
FaqI GGGAC 1 cut(s) 28
FatI CATG 3 cut(s) 63, 244, 457
FauI CCCGC 1 cut(s) 682
FauNDI CATATG 4 cut(s) 367, 565, 625, 910
FbaI TGATCA 1 cut(s) 330
Fnu4HI GCNGC 5 cut(s) 443, 482, 537, 666, 798
FokI GGATG 1 cut(s) 868
Fsp4HI GCNGC 5 cut(s) 443, 482, 537, 666, 798
GluI GCNGC 5 cut(s) 443, 482, 537, 666, 798
GsaI CCCAGC 1 cut(s) 735
GsuI CTGGAG 1 cut(s) 597
HaeIII GGCC 2 cut(s) 87, 442
HapII CCGG 1 cut(s) 533
Hin1II CATG 3 cut(s) 67, 248, 461
HinfI GANTC 3 cut(s) 212, 224, 304
HpaII CCGG 1 cut(s) 533
HphI GGTGA 2 cut(s) 94, 340
Hpy188I TCNGA 3 cut(s) 194, 405, 450
Hpy188III TCNNGA 4 cut(s) 95, 301, 346, 413
HpyAV CCTTC 4 cut(s) 311, 400, 702, 720
HpyCH4III ACNGT 2 cut(s) 359, 782
HpyCH4IV ACGT 1 cut(s) 162
HpyCH4V TGCA 9 cut(s) 470, 493, 518, 557, 581, 623, 629, 650, 800
HpyF10VI GCNNNNNNNGC 7 cut(s) 84, 490, 545, 554, 578, 587, 674
HpyF3I CTNAG 1 cut(s) 323
HpySE526I ACGT 1 cut(s) 162
Hsp92II CATG 3 cut(s) 67, 248, 461
Ksp22I TGATCA 1 cut(s) 330
Kzo9I GATC 3 cut(s) 154, 330, 415
LmnI GCTCC 2 cut(s) 569, 682
Lsp1109I GCAGC 3 cut(s) 548, 677, 784
LweI GCATC 1 cut(s) 626
MaeII ACGT 1 cut(s) 162
MaeIII GTNAC 3 cut(s) 100, 116, 361
MalI GATC 3 cut(s) 156, 332, 417
MbiI CCGCTC 1 cut(s) 677
MboI GATC 3 cut(s) 154, 330, 415
MboII GAAGA 4 cut(s) 21, 24, 30, 819
MflI RGATCY 1 cut(s) 154
MhlI GDGCHC 2 cut(s) 574, 787
MluCI AATT 7 cut(s) 14, 69, 217, 255, 296, 393, 915
MlyI GAGTC 1 cut(s) 218
MmeI TCCRAC 1 cut(s) 44
Mph1103I ATGCAT 1 cut(s) 495
MseI TTAA 2 cut(s) 254, 339
MspA1I CMGCKG 1 cut(s) 731
MspI CCGG 1 cut(s) 533
MspR9I CCNGG 1 cut(s) 756
Mva1269I GAATGC 1 cut(s) 78
MvaI CCWGG 1 cut(s) 756
MwoI GCNNNNNNNGC 7 cut(s) 84, 490, 545, 554, 578, 587, 674
NdeI CATATG 4 cut(s) 367, 565, 625, 910
NdeII GATC 3 cut(s) 154, 330, 415
NlaIII CATG 3 cut(s) 67, 248, 461
NlaIV GGNNCC 1 cut(s) 784
NmuCI GTSAC 2 cut(s) 100, 361
NsiI ATGCAT 1 cut(s) 495
PaqCI CACCTGC 1 cut(s) 586
PctI GAATGC 1 cut(s) 78
PfeI GAWTC 2 cut(s) 212, 304
Pfl23II CGTACG 1 cut(s) 777
PflMI CCANNNNNTGG 1 cut(s) 566
PkrI GCNGC 5 cut(s) 444, 483, 538, 667, 799
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
PsiI TTATAA 1 cut(s) 488
Psp6I CCWGG 1 cut(s) 754
PspEI GGTNACC 1 cut(s) 100
PspFI CCCAGC 1 cut(s) 731
PspGI CCWGG 1 cut(s) 754
PspLI CGTACG 1 cut(s) 777
PspN4I GGNNCC 1 cut(s) 784
PspPI GGNCC 1 cut(s) 905
PstNI CAGNNNCTG 1 cut(s) 620
PsuI RGATCY 1 cut(s) 154
PvuII CAGCTG 1 cut(s) 731
RsaI GTAC 3 cut(s) 436, 599, 779
RsaNI GTAC 3 cut(s) 435, 598, 778
SaqAI TTAA 2 cut(s) 254, 339
SatI GCNGC 5 cut(s) 443, 482, 537, 666, 798
Sau3AI GATC 3 cut(s) 154, 330, 415
Sau96I GGNCC 1 cut(s) 905
ScaI AGTACT 1 cut(s) 436
SchI GAGTC 1 cut(s) 218
ScrFI CCNGG 1 cut(s) 756
SduI GDGCHC 2 cut(s) 574, 787
SfaNI GCATC 1 cut(s) 626
SinI GGWCC 1 cut(s) 905
SmlI CTYRAG 2 cut(s) 95, 900
SmoI CTYRAG 2 cut(s) 95, 900
Sse9I AATT 7 cut(s) 14, 69, 217, 255, 296, 393, 915
SsiI CCGC 3 cut(s) 443, 481, 675
StyD4I CCNGG 1 cut(s) 754
TaaI ACNGT 2 cut(s) 359, 782
TaiI ACGT 1 cut(s) 165
TaqI TCGA 2 cut(s) 215, 227
TasI AATT 7 cut(s) 14, 69, 217, 255, 296, 393, 915
TatI WGTACW 2 cut(s) 434, 597
TauI GCSGC 2 cut(s) 445, 484
TfiI GAWTC 2 cut(s) 212, 304
Tru1I TTAA 2 cut(s) 254, 339
Tru9I TTAA 2 cut(s) 254, 339
TscAI CASTG 1 cut(s) 364
TseFI GTSAC 2 cut(s) 100, 361
TseI GCWGC 3 cut(s) 536, 665, 797
Tsp45I GTSAC 2 cut(s) 100, 361
TspDTI ATGAA 2 cut(s) 690, 702
TspRI CASTG 1 cut(s) 364
Van91I CCANNNNNTGG 1 cut(s) 566
VpaK11BI GGWCC 1 cut(s) 905
XapI RAATTY 4 cut(s) 217, 296, 393, 915
ZrmI AGTACT 1 cut(s) 436
Zsp2I ATGCAT 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.