Rroxscaffold_7G00209700

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0011916
Physical Location & Seq
Forward (+)
59942329 .. 59945037
2709 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00209700.1

Sequence Viewer

Length: 891 bp
ATGTCGGTTTCTGGCATTCAAGGCCAGCTTCTTGAGGTCACTGTTGTTGGGTGCAATAAGTTGAAAGATACAGAATGGATTTCGCGGCAAGATCCCTACGTTTGTGTTGAATATGCTAACTCCAAGCACAGAACAAGAACGCATACTGGTTCAGATCCGAATTTGGACTCGAACTGGACTGAAAGCCATGTTGATTTTAATGGTGTAGACGGTGGTAGAAACCCTACATTCCAAGAGAAGTTCGTGATTCCTCTAATTGAAGGGCTTAGGGAGATGAATGTTAATGTCTGGAACAGCAACAGTGTCACATATGACGACTTTATTGGCGAGGGAAAAGTTCACCTTCAGAAGGTTCTGTCTCAGGGTTACGATGATGGTACTTGGCCACTTCAAACTAAAACGGGCAGATATGCTGGAGAAGTGCGGCTTATAATGCATTACTCGAAGCCTGATATAAGTGCAAAGCCTAATAAACCGGCAGCAGGCTATGCCCCATCTGCACCACCTTATGGAGCACCAGCTGCACCGCAAGCTCATATGTACTCAACTCCCCCTCCAGCATCTGCATATGCACCACCACCTCGAATGTACGCACCACCTCCGCCAGCAGCTTACCCAGCTCCAGCTCCATCTCCCTATCCTTCGTATTCACCTACTCCATATCCACCCAATCCAGTACCAGCTGCGTATCCACCTAATCCCGCACCAGGAGGATATCCACCATCCCCGTACGGTGCCCCTCCACCAGCTGCATATCCTCCTGCACCATACCCACCATCTTCAACTTATCCTCCTCCTCCATCATCTACATATCCACCACCTCCACAAGCCTCATCGTTTTACCCTCCACCTCAAGGTCCGTATGGAGTATATCCACCACCACCATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04620 map04620
Pfam Domains
Protein Families
C2

Protein Analysis

296

Amino Acids

31.88

Weight (kDa)

6.29

Isoelectric Point (pI)

72.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 10 - 122 1e-10 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011916)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34150
fragaria_vesca FvH4_2g05480
malus_domestica MD00G1045300.v1.1 MD05G1087200.v1.1
prunus_persica Prupe.8G131800_v2.0.a1 Prupe.8G131800_v2.0.a1
pyrus_communis pycom05g08650 pycom10g08200
rosa_chinensis RchiOBHm_Chr6g0255751
rosa_laevigata RLG00000014816
rosa_multiflora Rmu_sc0001163.1_g000008 Rmu_sc0001163.1_g000009
rosa_roxburghii Rroxscaffold_7G00209700
rosa_rugosa Rorug05G0561000 Rorug05G0561100
rosa_samantha Rh6BG072200 Rh6CG070400 Rh6DG067600
rosa_wichuraiana Rw6G006820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 431
AccB1I GGYRCC 1 cut(s) 734
AccB7I CCANNNNNTGG 1 cut(s) 509
AccI GTMKAC 1 cut(s) 207
AccII CGCG 1 cut(s) 85
AciI CCGC 5 cut(s) 85, 424, 527, 602, 702
AclWI GGATC 2 cut(s) 86, 149
AcoI YGGCCR 1 cut(s) 383
AcsI RAATTY 1 cut(s) 160
AcuI CTGAAG 1 cut(s) 329
AfaI GTAC 5 cut(s) 379, 542, 590, 678, 731
AfiI CCNNNNNNNGG 5 cut(s) 349, 482, 509, 707, 854
AgsI TTSAA 6 cut(s) 20, 64, 110, 260, 392, 783
AjnI CCWGG 1 cut(s) 706
AluBI AGCT 8 cut(s) 28, 521, 533, 611, 620, 626, 683, 749
AluI AGCT 8 cut(s) 28, 521, 533, 611, 620, 626, 683, 749
Alw21I GWGCWC 1 cut(s) 517
Alw26I GTCTC 1 cut(s) 363
AlwI GGATC 2 cut(s) 86, 149
AlwNI CAGNNNCTG 1 cut(s) 563
AoxI GGCC 2 cut(s) 22, 383
ApeKI GCWGC 5 cut(s) 479, 521, 608, 683, 749
ApoI RAATTY 1 cut(s) 160
AspS9I GGNCC 1 cut(s) 857
AsuHPI GGTGA 2 cut(s) 332, 642
AvaII GGWCC 1 cut(s) 857
BaeGI GKGCMC 1 cut(s) 739
BalI TGGCCA 1 cut(s) 385
BanI GGYRCC 1 cut(s) 734
Bbv12I GWGCWC 1 cut(s) 517
BbvI GCAGC 5 cut(s) 491, 508, 620, 670, 736
BccI CCATC 6 cut(s) 368, 502, 637, 730, 784, 808
BciT130I CCWGG 1 cut(s) 708
BciVI GTATCC 1 cut(s) 699
BcoDI GTCTC 1 cut(s) 363
BfuI GTATCC 1 cut(s) 699
BisI GCNGC 7 cut(s) 86, 425, 480, 522, 609, 684, 750
BlsI GCNGC 7 cut(s) 87, 426, 481, 523, 610, 685, 751
Bme1390I CCNGG 1 cut(s) 708
Bme18I GGWCC 1 cut(s) 857
BmgT120I GGNCC 1 cut(s) 857
BmiI GGNNCC 1 cut(s) 736
BmrFI CCNGG 1 cut(s) 708
BmsI GCATC 1 cut(s) 569
BpmI CTGGAG 3 cut(s) 435, 540, 606
Bpu10I CCTNAGC 1 cut(s) 266
BpuEI CTTGAG 2 cut(s) 53, 837
BsaXI ACNNNNNCTCC 4 cut(s) 538, 568, 775, 805
Bsc4I CCNNNNNNNGG 5 cut(s) 349, 482, 509, 707, 854
Bse118I RCCGGY 1 cut(s) 475
Bse1I ACTGG 3 cut(s) 151, 179, 674
BseBI CCWGG 1 cut(s) 708
BseGI GGATG 1 cut(s) 722
BseLI CCNNNNNNNGG 5 cut(s) 349, 482, 509, 707, 854
BseMII CTCAG 1 cut(s) 374
BseNI ACTGG 3 cut(s) 151, 179, 674
BseRI GAGGAG 2 cut(s) 783, 786
BseSI GKGCMC 1 cut(s) 739
BseXI GCAGC 5 cut(s) 491, 508, 620, 670, 736
BseYI CCCAGC 1 cut(s) 616
BsgI GTGCAG 3 cut(s) 483, 507, 747
Bsh1236I CGCG 1 cut(s) 85
BshFI GGCC 2 cut(s) 24, 385
BshNI GGYRCC 1 cut(s) 734
BsiHKAI GWGCWC 1 cut(s) 517
BsiSI CCGG 1 cut(s) 476
BsiWI CGTACG 1 cut(s) 729
BslI CCNNNNNNNGG 5 cut(s) 349, 482, 509, 707, 854
BsmAI GTCTC 1 cut(s) 363
BsmI GAATGC 1 cut(s) 15
BsnI GGCC 2 cut(s) 24, 385
Bsp1286I GDGCHC 2 cut(s) 517, 739
Bsp143I GATC 2 cut(s) 91, 154
BspACI CCGC 5 cut(s) 85, 424, 527, 602, 702
BspANI GGCC 2 cut(s) 24, 385
BspCNI CTCAG 1 cut(s) 373
BspFNI CGCG 1 cut(s) 85
BspLI GGNNCC 1 cut(s) 736
BspPI GGATC 2 cut(s) 86, 149
BspT107I GGYRCC 1 cut(s) 734
BsrFI RCCGGY 1 cut(s) 475
BsrI ACTGG 3 cut(s) 151, 179, 674
BssAI RCCGGY 1 cut(s) 475
BssMI GATC 2 cut(s) 91, 154
Bst2UI CCWGG 1 cut(s) 708
Bst4CI ACNGT 4 cut(s) 43, 212, 302, 734
BstAPI GCANNNNNTGC 2 cut(s) 488, 521
BstC8I GCNNGC 4 cut(s) 26, 484, 531, 606
BstDEI CTNAG 2 cut(s) 266, 360
BstENI CCTNNNNNAGG 1 cut(s) 347
BstF5I GGATG 1 cut(s) 722
BstFNI CGCG 1 cut(s) 85
BstKTI GATC 2 cut(s) 94, 157
BstMAI GTCTC 1 cut(s) 363
BstMBI GATC 2 cut(s) 91, 154
BstMWI GCNNNNNNNGC 7 cut(s) 21, 433, 488, 497, 521, 530, 617
BstNI CCWGG 1 cut(s) 708
BstSCI CCNGG 1 cut(s) 706
BstSLI GKGCMC 1 cut(s) 739
BstUI CGCG 1 cut(s) 85
BstV1I GCAGC 5 cut(s) 491, 508, 620, 670, 736
BstX2I RGATCY 2 cut(s) 91, 154
BstYI RGATCY 2 cut(s) 91, 154
BsuI GTATCC 1 cut(s) 699
BsuRI GGCC 2 cut(s) 24, 385
BtsCI GGATG 1 cut(s) 722
BtsIMutI CAGTG 2 cut(s) 39, 307
Cac8I GCNNGC 4 cut(s) 26, 484, 531, 606
CaiI CAGNNNCTG 1 cut(s) 563
Cfr10I RCCGGY 1 cut(s) 475
Cfr13I GGNCC 1 cut(s) 857
Csp6I GTAC 5 cut(s) 378, 541, 589, 677, 730
CviAII CATG 1 cut(s) 188
CviQI GTAC 5 cut(s) 378, 541, 589, 677, 730
DdeI CTNAG 2 cut(s) 266, 360
DpnI GATC 2 cut(s) 93, 156
DpnII GATC 2 cut(s) 91, 154
EaeI YGGCCR 1 cut(s) 383
EciI GGCGGA 1 cut(s) 591
Eco32I GATATC 1 cut(s) 716
Eco47I GGWCC 1 cut(s) 857
Eco57I CTGAAG 1 cut(s) 329
EcoNI CCTNNNNNAGG 1 cut(s) 347
EcoRII CCWGG 1 cut(s) 706
EcoRV GATATC 1 cut(s) 716
EcoT22I ATGCAT 1 cut(s) 438
FaeI CATG 1 cut(s) 191
FalI AAGNNNNNCTT 6 cut(s) 12, 44, 327, 359, 411, 443
FatI CATG 1 cut(s) 187
FauI CCCGC 1 cut(s) 709
FauNDI CATATG 3 cut(s) 310, 537, 568
FblI GTMKAC 1 cut(s) 207
Fnu4HI GCNGC 7 cut(s) 86, 425, 480, 522, 609, 684, 750
FokI GGATG 1 cut(s) 709
Fsp4HI GCNGC 7 cut(s) 86, 425, 480, 522, 609, 684, 750
GluI GCNGC 7 cut(s) 86, 425, 480, 522, 609, 684, 750
GsaI CCCAGC 1 cut(s) 620
GsuI CTGGAG 3 cut(s) 435, 540, 606
HaeIII GGCC 2 cut(s) 24, 385
HapII CCGG 1 cut(s) 476
Hin1II CATG 1 cut(s) 191
HinfI GANTC 2 cut(s) 167, 247
HpaII CCGG 1 cut(s) 476
HphI GGTGA 2 cut(s) 332, 642
Hpy166II GTNNAC 2 cut(s) 208, 340
Hpy188I TCNGA 3 cut(s) 154, 159, 348
Hpy188III TCNNGA 3 cut(s) 32, 244, 289
Hpy8I GTNNAC 2 cut(s) 208, 340
HpyAV CCTTC 4 cut(s) 254, 343, 353, 651
HpyCH4III ACNGT 4 cut(s) 43, 212, 302, 734
HpyCH4IV ACGT 1 cut(s) 99
HpyCH4V TGCA 9 cut(s) 54, 436, 461, 500, 524, 566, 572, 752, 764
HpyF10VI GCNNNNNNNGC 7 cut(s) 21, 433, 488, 497, 521, 530, 617
HpyF3I CTNAG 2 cut(s) 266, 360
HpySE526I ACGT 1 cut(s) 99
Hsp92II CATG 1 cut(s) 191
Kzo9I GATC 2 cut(s) 91, 154
LmnI GCTCC 3 cut(s) 512, 625, 631
Lsp1109I GCAGC 5 cut(s) 491, 508, 620, 670, 736
LweI GCATC 1 cut(s) 569
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 3 cut(s) 37, 304, 365
MalI GATC 2 cut(s) 93, 156
MboI GATC 2 cut(s) 91, 154
MboII GAAGA 1 cut(s) 771
MflI RGATCY 2 cut(s) 91, 154
MhlI GDGCHC 2 cut(s) 517, 739
MlsI TGGCCA 1 cut(s) 385
MluCI AATT 2 cut(s) 160, 255
MluNI TGGCCA 1 cut(s) 385
MlyI GAGTC 1 cut(s) 161
Mox20I TGGCCA 1 cut(s) 385
Mph1103I ATGCAT 1 cut(s) 438
MscI TGGCCA 1 cut(s) 385
MseI TTAA 2 cut(s) 198, 282
Msp20I TGGCCA 1 cut(s) 385
MspA1I CMGCKG 3 cut(s) 521, 683, 749
MspI CCGG 1 cut(s) 476
MspR9I CCNGG 1 cut(s) 708
Mva1269I GAATGC 1 cut(s) 15
MvaI CCWGG 1 cut(s) 708
MvnI CGCG 1 cut(s) 85
MwoI GCNNNNNNNGC 7 cut(s) 21, 433, 488, 497, 521, 530, 617
NdeI CATATG 3 cut(s) 310, 537, 568
NdeII GATC 2 cut(s) 91, 154
NlaIII CATG 1 cut(s) 191
NlaIV GGNNCC 1 cut(s) 736
NmuCI GTSAC 2 cut(s) 37, 304
NsiI ATGCAT 1 cut(s) 438
PctI GAATGC 1 cut(s) 15
PfeI GAWTC 1 cut(s) 247
Pfl23II CGTACG 1 cut(s) 729
PflMI CCANNNNNTGG 1 cut(s) 509
PkrI GCNGC 7 cut(s) 87, 426, 481, 523, 610, 685, 751
PleI GAGTC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 161
PsiI TTATAA 1 cut(s) 431
Psp6I CCWGG 1 cut(s) 706
PspFI CCCAGC 1 cut(s) 616
PspGI CCWGG 1 cut(s) 706
PspLI CGTACG 1 cut(s) 729
PspN4I GGNNCC 1 cut(s) 736
PspPI GGNCC 1 cut(s) 857
PstNI CAGNNNCTG 1 cut(s) 563
PsuI RGATCY 2 cut(s) 91, 154
PvuII CAGCTG 3 cut(s) 521, 683, 749
RsaI GTAC 5 cut(s) 379, 542, 590, 678, 731
RsaNI GTAC 5 cut(s) 378, 541, 589, 677, 730
SaqAI TTAA 2 cut(s) 198, 282
SatI GCNGC 7 cut(s) 86, 425, 480, 522, 609, 684, 750
Sau3AI GATC 2 cut(s) 91, 154
Sau96I GGNCC 1 cut(s) 857
SchI GAGTC 1 cut(s) 161
ScrFI CCNGG 1 cut(s) 708
SduI GDGCHC 2 cut(s) 517, 739
SfaNI GCATC 1 cut(s) 569
SinI GGWCC 1 cut(s) 857
SmlI CTYRAG 2 cut(s) 32, 852
SmoI CTYRAG 2 cut(s) 32, 852
Sse9I AATT 2 cut(s) 160, 255
SsiI CCGC 5 cut(s) 85, 424, 527, 602, 702
StyD4I CCNGG 1 cut(s) 706
TaaI ACNGT 4 cut(s) 43, 212, 302, 734
TaiI ACGT 1 cut(s) 102
TaqI TCGA 3 cut(s) 170, 443, 583
TasI AATT 2 cut(s) 160, 255
TatI WGTACW 1 cut(s) 540
TauI GCSGC 2 cut(s) 88, 427
TfiI GAWTC 1 cut(s) 247
Tru1I TTAA 2 cut(s) 198, 282
Tru9I TTAA 2 cut(s) 198, 282
TscAI CASTG 2 cut(s) 46, 307
TseFI GTSAC 2 cut(s) 37, 304
TseI GCWGC 5 cut(s) 479, 521, 608, 683, 749
Tsp45I GTSAC 2 cut(s) 37, 304
TspDTI ATGAA 1 cut(s) 290
TspGWI ACGGA 1 cut(s) 849
TspRI CASTG 2 cut(s) 46, 307
Van91I CCANNNNNTGG 1 cut(s) 509
VpaK11BI GGWCC 1 cut(s) 857
XagI CCTNNNNNAGG 1 cut(s) 347
XapI RAATTY 1 cut(s) 160
XmiI GTMKAC 1 cut(s) 207
Zsp2I ATGCAT 1 cut(s) 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.