RchiOBHm_Chr6g0255751

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0011916
Physical Location & Seq
Reverse (-)
11164569 .. 11167562
2994 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22937

Sequence Viewer

Length: 897 bp
ATGTCATATTATCAATTTCCAACCCAGTCTTCTTCGTCTTCGTCTCCTTACTCCTCTTTTCCCATGTCGACTTCTGGTATTCAAGGCCAGCTTCTTGAGGTCACTGTTGTTGGGTGCAATAAGTTGAAAGATACAGAATGGATTTCGCGGCAAGATCCCTACGTTTGTGTTGAATATGCTAACTCCAAGCACAGAACAAGAACGCATACTGACGGAGGTAGAAACCCTACATTCCAAGAGAAGTTCGTGATTCCTCTAATTGAAGGGCTTAGGGAGATGAATGTTGCTGTCTGGAACAGCAACAGTGTCACATATGACGACTTTATTGGCGAGGGAAAAGTACACCTTCAGAAGGTTCTGTCTCAGGGTTACGATGATGGTACTTGGCCACTTCAGACTAAAACGGGCAGATATGCTGGAGAAGTGCGGCTTATAATGCATTACTCGAAGCCTGATATAAGTGCAAAGCCTAATAAACTGGCAGCAGGCTATGCCCCATCTGCACCACCTTATGGAGCACCACCTGCACCGCAAGCTCATATGTACTCAACTCCCCCTCCAGCATCTGCGTATGCACCACCACCTCAAATGTATGCACCACCTCCACCAGCAGCTTACCCAGCTCCAGCTCCATCTCCCTATCCTTCGTATTCACCTACTCCTTCATATCCACCCAATCCAGCACCAGCTGCGTATCCACCTAATCCCGCACCAGGAGGATATCCACCATCCCCGTACGGTGCCCCTCCACCAGCTGCATATCCTCCTGCACCATACCCACCATCTTCAACTTATCCTCCTCCTCCATCATCTACATATCCACCACCTCCACAAGCCTCATCGTTTTACCCTCCACCTCAAGGTCCGTATGGAGTATATCCACCACCACCATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04620 map04620
Pfam Domains
Protein Families
C2

Protein Analysis

298

Amino Acids

32.09

Weight (kDa)

7.68

Isoelectric Point (pI)

83.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 31 - 123 6.6e-16 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011916)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34150
fragaria_vesca FvH4_2g05480
malus_domestica MD00G1045300.v1.1 MD05G1087200.v1.1
prunus_persica Prupe.8G131800_v2.0.a1 Prupe.8G131800_v2.0.a1
pyrus_communis pycom05g08650 pycom10g08200
rosa_chinensis RchiOBHm_Chr6g0255751
rosa_laevigata RLG00000014816
rosa_multiflora Rmu_sc0001163.1_g000008 Rmu_sc0001163.1_g000009
rosa_roxburghii Rroxscaffold_7G00209700
rosa_rugosa Rorug05G0561000 Rorug05G0561100
rosa_samantha Rh6BG072200 Rh6CG070400 Rh6DG067600
rosa_wichuraiana Rw6G006820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 434
AarI CACCTGC 1 cut(s) 532
Acc36I ACCTGC 1 cut(s) 532
AccB1I GGYRCC 1 cut(s) 740
AccB7I CCANNNNNTGG 1 cut(s) 512
AccI GTMKAC 1 cut(s) 68
AccII CGCG 1 cut(s) 148
AciI CCGC 4 cut(s) 148, 427, 530, 708
AclWI GGATC 1 cut(s) 149
AcoI YGGCCR 1 cut(s) 386
AcuI CTGAAG 2 cut(s) 332, 377
AfaI GTAC 4 cut(s) 342, 382, 545, 737
AfiI CCNNNNNNNGG 4 cut(s) 352, 512, 713, 860
AgsI TTSAA 5 cut(s) 83, 127, 173, 263, 789
AjnI CCWGG 1 cut(s) 712
AjuI GAANNNNNNNTTGG 2 cut(s) 13, 45
AluBI AGCT 7 cut(s) 91, 536, 614, 623, 629, 689, 755
AluI AGCT 7 cut(s) 91, 536, 614, 623, 629, 689, 755
Alw21I GWGCWC 1 cut(s) 520
Alw26I GTCTC 2 cut(s) 48, 366
AlwI GGATC 1 cut(s) 149
AlwNI CAGNNNCTG 1 cut(s) 566
AoxI GGCC 2 cut(s) 85, 386
ApeKI GCWGC 4 cut(s) 482, 611, 689, 755
AspS9I GGNCC 1 cut(s) 863
AsuHPI GGTGA 1 cut(s) 645
AvaII GGWCC 1 cut(s) 863
BaeGI GKGCMC 1 cut(s) 745
BalI TGGCCA 1 cut(s) 388
BanI GGYRCC 1 cut(s) 740
BbsI GAAGAC 2 cut(s) 21, 30
Bbv12I GWGCWC 1 cut(s) 520
BbvI GCAGC 4 cut(s) 494, 623, 676, 742
BccI CCATC 6 cut(s) 371, 505, 640, 736, 790, 814
BciT130I CCWGG 1 cut(s) 714
BciVI GTATCC 1 cut(s) 705
BcoDI GTCTC 2 cut(s) 48, 366
BfuAI ACCTGC 1 cut(s) 532
BfuI GTATCC 1 cut(s) 705
BisI GCNGC 6 cut(s) 149, 428, 483, 612, 690, 756
BlsI GCNGC 6 cut(s) 150, 429, 484, 613, 691, 757
Bme1390I CCNGG 1 cut(s) 714
Bme18I GGWCC 1 cut(s) 863
BmgT120I GGNCC 1 cut(s) 863
BmiI GGNNCC 1 cut(s) 742
BmrFI CCNGG 1 cut(s) 714
BmrI ACTGGG 1 cut(s) 19
BmsI GCATC 1 cut(s) 572
BmuI ACTGGG 1 cut(s) 19
BpiI GAAGAC 2 cut(s) 21, 30
BpmI CTGGAG 3 cut(s) 438, 543, 609
Bpu10I CCTNAGC 1 cut(s) 269
BpuEI CTTGAG 2 cut(s) 116, 843
BsaXI ACNNNNNCTCC 4 cut(s) 541, 571, 781, 811
Bsc4I CCNNNNNNNGG 4 cut(s) 352, 512, 713, 860
Bse1I ACTGG 2 cut(s) 25, 483
BseBI CCWGG 1 cut(s) 714
BseGI GGATG 1 cut(s) 728
BseLI CCNNNNNNNGG 4 cut(s) 352, 512, 713, 860
BseMII CTCAG 1 cut(s) 377
BseNI ACTGG 2 cut(s) 25, 483
BseRI GAGGAG 3 cut(s) 43, 789, 792
BseSI GKGCMC 1 cut(s) 745
BseXI GCAGC 4 cut(s) 494, 623, 676, 742
BseYI CCCAGC 1 cut(s) 619
BsgI GTGCAG 3 cut(s) 486, 510, 753
Bsh1236I CGCG 1 cut(s) 148
BshFI GGCC 2 cut(s) 87, 388
BshNI GGYRCC 1 cut(s) 740
BsiHKAI GWGCWC 1 cut(s) 520
BsiWI CGTACG 1 cut(s) 735
BslI CCNNNNNNNGG 4 cut(s) 352, 512, 713, 860
BsmAI GTCTC 2 cut(s) 48, 366
BsmBI CGTCTC 1 cut(s) 48
BsnI GGCC 2 cut(s) 87, 388
Bsp1286I GDGCHC 2 cut(s) 520, 745
Bsp143I GATC 1 cut(s) 154
BspACI CCGC 4 cut(s) 148, 427, 530, 708
BspANI GGCC 2 cut(s) 87, 388
BspCNI CTCAG 1 cut(s) 376
BspFNI CGCG 1 cut(s) 148
BspLI GGNNCC 1 cut(s) 742
BspMI ACCTGC 1 cut(s) 532
BspPI GGATC 1 cut(s) 149
BspT107I GGYRCC 1 cut(s) 740
BsrI ACTGG 2 cut(s) 25, 483
BssMI GATC 1 cut(s) 154
Bst2UI CCWGG 1 cut(s) 714
Bst4CI ACNGT 3 cut(s) 106, 305, 740
BstAPI GCANNNNNTGC 3 cut(s) 491, 524, 689
BstC8I GCNNGC 3 cut(s) 89, 487, 534
BstDEI CTNAG 2 cut(s) 269, 363
BstENI CCTNNNNNAGG 1 cut(s) 350
BstF5I GGATG 1 cut(s) 728
BstFNI CGCG 1 cut(s) 148
BstKTI GATC 1 cut(s) 157
BstMAI GTCTC 2 cut(s) 48, 366
BstMBI GATC 1 cut(s) 154
BstMWI GCNNNNNNNGC 7 cut(s) 436, 491, 500, 524, 533, 620, 689
BstNI CCWGG 1 cut(s) 714
BstSCI CCNGG 1 cut(s) 712
BstSLI GKGCMC 1 cut(s) 745
BstUI CGCG 1 cut(s) 148
BstV1I GCAGC 4 cut(s) 494, 623, 676, 742
BstV2I GAAGAC 2 cut(s) 21, 30
BstX2I RGATCY 1 cut(s) 154
BstYI RGATCY 1 cut(s) 154
BsuI GTATCC 1 cut(s) 705
BsuRI GGCC 2 cut(s) 87, 388
BtsCI GGATG 1 cut(s) 728
BtsIMutI CAGTG 2 cut(s) 102, 310
BveI ACCTGC 1 cut(s) 532
Cac8I GCNNGC 3 cut(s) 89, 487, 534
CaiI CAGNNNCTG 1 cut(s) 566
Cfr13I GGNCC 1 cut(s) 863
Csp6I GTAC 4 cut(s) 341, 381, 544, 736
CviAII CATG 1 cut(s) 64
CviQI GTAC 4 cut(s) 341, 381, 544, 736
DdeI CTNAG 2 cut(s) 269, 363
DpnI GATC 1 cut(s) 156
DpnII GATC 1 cut(s) 154
EaeI YGGCCR 1 cut(s) 386
Eco32I GATATC 1 cut(s) 722
Eco47I GGWCC 1 cut(s) 863
Eco57I CTGAAG 2 cut(s) 332, 377
EcoNI CCTNNNNNAGG 1 cut(s) 350
EcoRII CCWGG 1 cut(s) 712
EcoRV GATATC 1 cut(s) 722
EcoT22I ATGCAT 1 cut(s) 441
Esp3I CGTCTC 1 cut(s) 48
FaeI CATG 1 cut(s) 67
FalI AAGNNNNNCTT 6 cut(s) 75, 107, 330, 362, 414, 446
FatI CATG 1 cut(s) 63
FauI CCCGC 1 cut(s) 715
FauNDI CATATG 2 cut(s) 313, 540
FblI GTMKAC 1 cut(s) 68
Fnu4HI GCNGC 6 cut(s) 149, 428, 483, 612, 690, 756
FokI GGATG 1 cut(s) 715
Fsp4HI GCNGC 6 cut(s) 149, 428, 483, 612, 690, 756
GluI GCNGC 6 cut(s) 149, 428, 483, 612, 690, 756
GsaI CCCAGC 1 cut(s) 623
GsuI CTGGAG 3 cut(s) 438, 543, 609
HaeIII GGCC 2 cut(s) 87, 388
Hin1II CATG 1 cut(s) 67
HincII GTYRAC 1 cut(s) 69
HindII GTYRAC 1 cut(s) 69
HinfI GANTC 1 cut(s) 250
HphI GGTGA 1 cut(s) 645
Hpy166II GTNNAC 2 cut(s) 69, 343
Hpy188I TCNGA 2 cut(s) 351, 396
Hpy188III TCNNGA 3 cut(s) 95, 247, 292
Hpy8I GTNNAC 2 cut(s) 69, 343
HpyAV CCTTC 5 cut(s) 257, 346, 356, 654, 672
HpyCH4III ACNGT 3 cut(s) 106, 305, 740
HpyCH4IV ACGT 1 cut(s) 162
HpyCH4V TGCA 9 cut(s) 117, 439, 464, 503, 527, 575, 596, 758, 770
HpyF10VI GCNNNNNNNGC 7 cut(s) 436, 491, 500, 524, 533, 620, 689
HpyF3I CTNAG 2 cut(s) 269, 363
HpySE526I ACGT 1 cut(s) 162
Hsp92II CATG 1 cut(s) 67
Kzo9I GATC 1 cut(s) 154
LmnI GCTCC 3 cut(s) 515, 628, 634
Lsp1109I GCAGC 4 cut(s) 494, 623, 676, 742
LweI GCATC 1 cut(s) 572
MaeII ACGT 1 cut(s) 162
MaeIII GTNAC 3 cut(s) 100, 307, 368
MalI GATC 1 cut(s) 156
MboI GATC 1 cut(s) 154
MboII GAAGA 4 cut(s) 21, 24, 30, 777
MflI RGATCY 1 cut(s) 154
MhlI GDGCHC 2 cut(s) 520, 745
MlsI TGGCCA 1 cut(s) 388
MluCI AATT 2 cut(s) 14, 258
MluNI TGGCCA 1 cut(s) 388
MmeI TCCRAC 1 cut(s) 44
Mox20I TGGCCA 1 cut(s) 388
Mph1103I ATGCAT 1 cut(s) 441
MscI TGGCCA 1 cut(s) 388
Msp20I TGGCCA 1 cut(s) 388
MspA1I CMGCKG 2 cut(s) 689, 755
MspR9I CCNGG 1 cut(s) 714
MvaI CCWGG 1 cut(s) 714
MvnI CGCG 1 cut(s) 148
MwoI GCNNNNNNNGC 7 cut(s) 436, 491, 500, 524, 533, 620, 689
NdeI CATATG 2 cut(s) 313, 540
NdeII GATC 1 cut(s) 154
NlaIII CATG 1 cut(s) 67
NlaIV GGNNCC 1 cut(s) 742
NmuCI GTSAC 2 cut(s) 100, 307
NsiI ATGCAT 1 cut(s) 441
PaqCI CACCTGC 1 cut(s) 532
PfeI GAWTC 1 cut(s) 250
Pfl23II CGTACG 1 cut(s) 735
PflMI CCANNNNNTGG 1 cut(s) 512
PkrI GCNGC 6 cut(s) 150, 429, 484, 613, 691, 757
PsiI TTATAA 1 cut(s) 434
Psp6I CCWGG 1 cut(s) 712
PspFI CCCAGC 1 cut(s) 619
PspGI CCWGG 1 cut(s) 712
PspLI CGTACG 1 cut(s) 735
PspN4I GGNNCC 1 cut(s) 742
PspPI GGNCC 1 cut(s) 863
PstNI CAGNNNCTG 1 cut(s) 566
PsuI RGATCY 1 cut(s) 154
PvuII CAGCTG 2 cut(s) 689, 755
RsaI GTAC 4 cut(s) 342, 382, 545, 737
RsaNI GTAC 4 cut(s) 341, 381, 544, 736
SalI GTCGAC 1 cut(s) 67
SatI GCNGC 6 cut(s) 149, 428, 483, 612, 690, 756
Sau3AI GATC 1 cut(s) 154
Sau96I GGNCC 1 cut(s) 863
ScrFI CCNGG 1 cut(s) 714
SduI GDGCHC 2 cut(s) 520, 745
SfaNI GCATC 1 cut(s) 572
SinI GGWCC 1 cut(s) 863
SmlI CTYRAG 2 cut(s) 95, 858
SmoI CTYRAG 2 cut(s) 95, 858
Sse9I AATT 2 cut(s) 14, 258
SsiI CCGC 4 cut(s) 148, 427, 530, 708
StyD4I CCNGG 1 cut(s) 712
TaaI ACNGT 3 cut(s) 106, 305, 740
TaiI ACGT 1 cut(s) 165
TaqI TCGA 2 cut(s) 68, 446
TasI AATT 2 cut(s) 14, 258
TatI WGTACW 2 cut(s) 340, 543
TauI GCSGC 2 cut(s) 151, 430
TfiI GAWTC 1 cut(s) 250
TscAI CASTG 2 cut(s) 109, 310
TseFI GTSAC 2 cut(s) 100, 307
TseI GCWGC 4 cut(s) 482, 611, 689, 755
Tsp45I GTSAC 2 cut(s) 100, 307
TspDTI ATGAA 2 cut(s) 293, 654
TspGWI ACGGA 2 cut(s) 228, 855
TspRI CASTG 2 cut(s) 109, 310
Van91I CCANNNNNTGG 1 cut(s) 512
VpaK11BI GGWCC 1 cut(s) 863
XagI CCTNNNNNAGG 1 cut(s) 350
XmiI GTMKAC 1 cut(s) 68
Zsp2I ATGCAT 1 cut(s) 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.