Rorug05G0561100

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0011916
Physical Location & Seq
Reverse (-)
75133221 .. 75133832
612 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0561100.1

Sequence Viewer

Length: 612 bp
ATGGGCACTTTGTGTGTGCAATCGTTCCAGCATCCTTTTATGGTACGGGAGAGACCGGAGTCGTCGGAGAATCAGTACTTCTTGATGAATATCAGCTTTGTGACTGATCCGAATTATGGGGGTCCTGCCTCCTTGCTCCATCTTCAAACCGGTGACTTACTGAGGGAGAAGCCAATTGTGTCGAGGACTGTCATTGAAGGCGCGCTTTCTAGCTTGCGTGTTCCGTGCCGGTATCACCATTCCGTTGTAGAAAAAATACTAAGCGATGCAAAGGCAAGTGCCAAAGCCAAGGGTTTGAATTTGTGGGTTAAAGTCTACATGGATATTTATGGTCTTGGTCATTCTGACATGGAGGAGGAGGGCACATATTCGGACACGGAGGATGACATGGATTCGGATACGGAGGAGGATGACATGGATTCTAACATGGAGGAGGAGGAGGAGTCTGTTTTCTGTTCCGTTTGTAACGAGGAGATTGTGGTTGGTTCCGAAGAAACTCGTATCCCATGTACGCACATGTACCATAGAGATTGCATTGTCAGGTGGCTGCAAAGTAGTTATTGCCTACCGTGTAGGTATGGTATAAGCCAGCAGTTGATGAAGCCTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04620 map04620
Pfam Domains
Protein Families
C2

Protein Analysis

203

Amino Acids

23.17

Weight (kDa)

4.63

Isoelectric Point (pI)

60.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 152 - 192 1.1e-06 Ring finger domain
zf-RING_11 PF17123 152 - 179 4e-07 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011916)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34150
fragaria_vesca FvH4_2g05480
malus_domestica MD00G1045300.v1.1 MD05G1087200.v1.1
prunus_persica Prupe.8G131800_v2.0.a1 Prupe.8G131800_v2.0.a1
pyrus_communis pycom05g08650 pycom10g08200
rosa_chinensis RchiOBHm_Chr6g0255751
rosa_laevigata RLG00000014816
rosa_multiflora Rmu_sc0001163.1_g000008 Rmu_sc0001163.1_g000009
rosa_roxburghii Rroxscaffold_7G00209700
rosa_rugosa Rorug05G0561000 Rorug05G0561100
rosa_samantha Rh6BG072200 Rh6CG070400 Rh6DG067600
rosa_wichuraiana Rw6G006820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 315
AccII CGCG 1 cut(s) 203
AclWI GGATC 1 cut(s) 101
AcsI RAATTY 1 cut(s) 298
AdeI CACNNNGTG 1 cut(s) 12
AfaI GTAC 4 cut(s) 45, 77, 511, 521
AfiI CCNNNNNNNGG 1 cut(s) 116
AflIII ACRYGT 1 cut(s) 516
AgeI ACCGGT 1 cut(s) 149
AgsI TTSAA 3 cut(s) 146, 197, 298
AluBI AGCT 2 cut(s) 96, 213
AluI AGCT 2 cut(s) 96, 213
Alw26I GTCTC 1 cut(s) 46
AlwI GGATC 1 cut(s) 101
ApeKI GCWGC 1 cut(s) 547
ApoI RAATTY 1 cut(s) 298
AsiGI ACCGGT 1 cut(s) 149
AspLEI GCGC 2 cut(s) 203, 205
AspS9I GGNCC 1 cut(s) 122
AsuHPI GGTGA 2 cut(s) 164, 227
AvaII GGWCC 1 cut(s) 122
BaeGI GKGCMC 2 cut(s) 8, 365
BbvI GCAGC 1 cut(s) 534
BccI CCATC 1 cut(s) 147
BciVI GTATCC 2 cut(s) 391, 512
BcoDI GTCTC 1 cut(s) 46
BfaI CTAG 1 cut(s) 210
BfuI GTATCC 2 cut(s) 391, 512
BisI GCNGC 1 cut(s) 548
BlsI GCNGC 1 cut(s) 549
BmcAI AGTACT 1 cut(s) 77
Bme18I GGWCC 1 cut(s) 122
BmgT120I GGNCC 1 cut(s) 122
BmiI GGNNCC 2 cut(s) 123, 487
BmsI GCATC 2 cut(s) 40, 256
BoxI GACNNNNGTC 1 cut(s) 58
BsaBI GATNNNNATC 1 cut(s) 89
BsaI GGTCTC 1 cut(s) 46
BsaJI CCNNGG 1 cut(s) 288
BsaWI WCCGGW 2 cut(s) 55, 149
BsaXI ACNNNNNCTCC 4 cut(s) 59, 89, 428, 458
Bsc4I CCNNNNNNNGG 1 cut(s) 116
Bse118I RCCGGY 2 cut(s) 149, 228
Bse8I GATNNNNATC 1 cut(s) 89
BseDI CCNNGG 1 cut(s) 288
BseGI GGATG 3 cut(s) 31, 388, 415
BseJI GATNNNNATC 1 cut(s) 89
BseLI CCNNNNNNNGG 1 cut(s) 116
BseMII CTCAG 1 cut(s) 152
BsePI GCGCGC 1 cut(s) 201
BseRI GAGGAG 8 cut(s) 368, 371, 419, 446, 449, 452, 455, 485
BseSI GKGCMC 2 cut(s) 8, 365
BseXI GCAGC 1 cut(s) 534
Bsh1236I CGCG 1 cut(s) 203
BshTI ACCGGT 1 cut(s) 149
BsiSI CCGG 3 cut(s) 56, 150, 229
BslI CCNNNNNNNGG 1 cut(s) 116
BsmAI GTCTC 1 cut(s) 46
Bso31I GGTCTC 1 cut(s) 46
Bsp1286I GDGCHC 2 cut(s) 8, 365
Bsp143I GATC 1 cut(s) 106
BspCNI CTCAG 1 cut(s) 153
BspFNI CGCG 1 cut(s) 203
BspLI GGNNCC 2 cut(s) 123, 487
BspPI GGATC 1 cut(s) 101
BspTNI GGTCTC 1 cut(s) 46
BsrFI RCCGGY 2 cut(s) 149, 228
BssAI RCCGGY 2 cut(s) 149, 228
BssECI CCNNGG 1 cut(s) 288
BssHII GCGCGC 1 cut(s) 201
BssMI GATC 1 cut(s) 106
BssT1I CCWWGG 1 cut(s) 288
Bst4CI ACNGT 2 cut(s) 190, 570
BstC8I GCNNGC 3 cut(s) 203, 215, 590
BstDEI CTNAG 2 cut(s) 161, 260
BstF5I GGATG 3 cut(s) 31, 388, 415
BstFNI CGCG 1 cut(s) 203
BstHHI GCGC 2 cut(s) 203, 205
BstKTI GATC 1 cut(s) 109
BstMAI GTCTC 1 cut(s) 46
BstMBI GATC 1 cut(s) 106
BstNSI RCATGY 1 cut(s) 520
BstPAI GACNNNNGTC 1 cut(s) 58
BstSLI GKGCMC 2 cut(s) 8, 365
BstUI CGCG 1 cut(s) 203
BstV1I GCAGC 1 cut(s) 534
BsuI GTATCC 2 cut(s) 391, 512
BtgZI GCGATG 1 cut(s) 279
BtsCI GGATG 3 cut(s) 31, 388, 415
Cac8I GCNNGC 3 cut(s) 203, 215, 590
CfoI GCGC 2 cut(s) 203, 205
Cfr10I RCCGGY 2 cut(s) 149, 228
Cfr13I GGNCC 1 cut(s) 122
Csp6I GTAC 4 cut(s) 44, 76, 510, 520
CspAI ACCGGT 1 cut(s) 149
CviAII CATG 7 cut(s) 319, 349, 388, 415, 427, 507, 517
CviJI RGCY 7 cut(s) 96, 172, 213, 287, 547, 588, 604
CviKI_1 RGCY 7 cut(s) 96, 172, 213, 287, 547, 588, 604
CviQI GTAC 4 cut(s) 44, 76, 510, 520
DdeI CTNAG 2 cut(s) 161, 260
DpnI GATC 1 cut(s) 108
DpnII GATC 1 cut(s) 106
DraIII CACNNNGTG 1 cut(s) 12
Eco130I CCWWGG 1 cut(s) 288
Eco31I GGTCTC 1 cut(s) 46
Eco47I GGWCC 1 cut(s) 122
EcoO109I RGGNCCY 1 cut(s) 122
EcoT14I CCWWGG 1 cut(s) 288
ErhI CCWWGG 1 cut(s) 288
FaeI CATG 7 cut(s) 322, 352, 391, 418, 430, 510, 520
FalI AAGNNNNNCTT 2 cut(s) 189, 221
FatI CATG 7 cut(s) 318, 348, 387, 414, 426, 506, 516
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 1 cut(s) 548
FokI GGATG 3 cut(s) 18, 395, 422
Fsp4HI GCNGC 1 cut(s) 548
FspBI CTAG 1 cut(s) 210
GlaI GCGC 2 cut(s) 202, 204
GluI GCNGC 1 cut(s) 548
HapII CCGG 3 cut(s) 56, 150, 229
HhaI GCGC 2 cut(s) 203, 205
Hin1II CATG 7 cut(s) 322, 352, 391, 418, 430, 510, 520
Hin6I GCGC 2 cut(s) 201, 203
HinP1I GCGC 2 cut(s) 201, 203
HinfI GANTC 5 cut(s) 59, 70, 392, 419, 443
HpaII CCGG 3 cut(s) 56, 150, 229
HphI GGTGA 2 cut(s) 164, 227
Hpy166II GTNNAC 1 cut(s) 316
Hpy188I TCNGA 6 cut(s) 67, 111, 346, 373, 397, 490
Hpy188III TCNNGA 1 cut(s) 82
Hpy8I GTNNAC 1 cut(s) 316
Hpy99I CGWCG 1 cut(s) 67
HpyAV CCTTC 1 cut(s) 191
HpyCH4III ACNGT 2 cut(s) 190, 570
HpyCH4V TGCA 4 cut(s) 19, 269, 534, 550
HpyF3I CTNAG 2 cut(s) 161, 260
Hsp92II CATG 7 cut(s) 322, 352, 391, 418, 430, 510, 520
HspAI GCGC 2 cut(s) 201, 203
Kzo9I GATC 1 cut(s) 106
LmnI GCTCC 1 cut(s) 141
LpnPI CCDG 7 cut(s) 41, 69, 138, 163, 242, 526, 602
Lsp1109I GCAGC 1 cut(s) 534
LweI GCATC 2 cut(s) 40, 256
MaeI CTAG 1 cut(s) 210
MaeIII GTNAC 3 cut(s) 100, 152, 464
MalI GATC 1 cut(s) 108
MboI GATC 1 cut(s) 106
MboII GAAGA 2 cut(s) 134, 503
MfeI CAATTG 1 cut(s) 174
MhlI GDGCHC 2 cut(s) 8, 365
MluCI AATT 3 cut(s) 112, 174, 298
MlyI GAGTC 2 cut(s) 68, 452
MmeI TCCRAC 1 cut(s) 45
MseI TTAA 1 cut(s) 309
MspI CCGG 3 cut(s) 56, 150, 229
MunI CAATTG 1 cut(s) 174
MvnI CGCG 1 cut(s) 203
NdeII GATC 1 cut(s) 106
NlaIII CATG 7 cut(s) 322, 352, 391, 418, 430, 510, 520
NlaIV GGNNCC 2 cut(s) 123, 487
NmuCI GTSAC 2 cut(s) 100, 152
NspI RCATGY 1 cut(s) 520
PauI GCGCGC 1 cut(s) 201
PciI ACATGT 1 cut(s) 516
PfeI GAWTC 3 cut(s) 70, 392, 419
PinAI ACCGGT 1 cut(s) 149
PkrI GCNGC 1 cut(s) 549
PleI GAGTC 2 cut(s) 67, 451
PpsI GAGTC 2 cut(s) 67, 451
PpuMI RGGWCCY 1 cut(s) 122
PscI ACATGT 1 cut(s) 516
PshAI GACNNNNGTC 1 cut(s) 58
Psp5II RGGWCCY 1 cut(s) 122
PspN4I GGNNCC 2 cut(s) 123, 487
PspPI GGNCC 1 cut(s) 122
PspPPI RGGWCCY 1 cut(s) 122
PteI GCGCGC 1 cut(s) 201
RsaI GTAC 4 cut(s) 45, 77, 511, 521
RsaNI GTAC 4 cut(s) 44, 76, 510, 520
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 1 cut(s) 548
Sau3AI GATC 1 cut(s) 106
Sau96I GGNCC 1 cut(s) 122
ScaI AGTACT 1 cut(s) 77
SchI GAGTC 2 cut(s) 68, 452
SduI GDGCHC 2 cut(s) 8, 365
SetI ASST 4 cut(s) 98, 215, 545, 578
SfaNI GCATC 2 cut(s) 40, 256
SinI GGWCC 1 cut(s) 122
Sse9I AATT 3 cut(s) 112, 174, 298
SspMI CTAG 1 cut(s) 210
StyI CCWWGG 1 cut(s) 288
TaaI ACNGT 2 cut(s) 190, 570
TaqI TCGA 1 cut(s) 182
TasI AATT 3 cut(s) 112, 174, 298
TatI WGTACW 1 cut(s) 75
TfiI GAWTC 3 cut(s) 70, 392, 419
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TseFI GTSAC 2 cut(s) 100, 152
TseI GCWGC 1 cut(s) 547
Tsp45I GTSAC 2 cut(s) 100, 152
TspDTI ATGAA 1 cut(s) 101
TspGWI ACGGA 5 cut(s) 213, 232, 392, 416, 448
VpaK11BI GGWCC 1 cut(s) 122
XapI RAATTY 1 cut(s) 298
XceI RCATGY 1 cut(s) 520
XmiI GTMKAC 1 cut(s) 315
XspI CTAG 1 cut(s) 210
ZrmI AGTACT 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.