RLG00000014816

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0011916
Physical Location & Seq
Forward (+)
57925188 .. 57927516
2329 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014816

Sequence Viewer

Length: 840 bp
ATGTCGACTTCTGGAATTCAAGGCCAGCTTCTTGAGGTCACTGTTGTTGGGTGCAATAAGTTGAAAGATACAGAATGGATTTCGCGGCAAGATCCCTACGTTTGTGTTGAATATGCTAATTCAAAGCACAGAACAAGAACGCATACTGACGGAGGTAGAAACCCTACATTCCAAGAGAAGTTTGTGATTCCTTTAATTGAAGGGCTTAGGGAGATGAATGTTAATGTCTGGAACAGCAACAGTGTCACATATGACGACTTTATTGGCGAGGGAAAAGTTCACCTTCAGAAGGTTCTGTCTCAGGGTTACGATGATGGTACTTGGCCACTTCAGACTAAAACGGGCAGATATGCTGGAGAAGTGCGGCTTATAATGCATTACTCGAAGCCTGATATAAGTGCAAAGCCTAATAAACCGGCAGCAGGCTATGCCCCATCTGCACCACCTTATGGAGCACCACCTGCACTGCAAGCTCATATGTACTCAACTCCCCCTCCAGCATCTGCGTATGCACCACCACCTCAAATGTACGCACCACCTCCACCAGCAGCTTACCCAGCTCCAGCTCCATCTCCCTATCCTTCGTATTCACCTACTCCTTCATATCCACCCAATCCAGCACCAGCTGCGTATCCACCTAATCCCGCACCAGGAGGATATCCACCATCCCCGTACGGTGCCCCTCCACCAGCTGCATATCCTCCTGCACCATACCCACCATCTTCAACTTATCCTCCTCCTCCATCATCTACATATCCACCACCTCCACAAGCCTCATCGTTTTACCCTCCACCTCGAGGTCCGTATGGAGTATATCCACCACCACCACCACCGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04620 map04620
Pfam Domains
Protein Families
C2

Protein Analysis

280

Amino Acids

30.01

Weight (kDa)

8.31

Isoelectric Point (pI)

79.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 10 - 102 4.2e-16 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011916)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34150
fragaria_vesca FvH4_2g05480
malus_domestica MD00G1045300.v1.1 MD05G1087200.v1.1
prunus_persica Prupe.8G131800_v2.0.a1 Prupe.8G131800_v2.0.a1
pyrus_communis pycom05g08650 pycom10g08200
rosa_chinensis RchiOBHm_Chr6g0255751
rosa_laevigata RLG00000014816
rosa_multiflora Rmu_sc0001163.1_g000008 Rmu_sc0001163.1_g000009
rosa_roxburghii Rroxscaffold_7G00209700
rosa_rugosa Rorug05G0561000 Rorug05G0561100
rosa_samantha Rh6BG072200 Rh6CG070400 Rh6DG067600
rosa_wichuraiana Rw6G006820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 371
AarI CACCTGC 1 cut(s) 469
AbsI CCTCGAGG 1 cut(s) 795
Acc36I ACCTGC 1 cut(s) 469
AccB1I GGYRCC 1 cut(s) 677
AccB7I CCANNNNNTGG 1 cut(s) 449
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 85
AciI CCGC 3 cut(s) 85, 364, 645
AclWI GGATC 1 cut(s) 86
AcoI YGGCCR 1 cut(s) 323
AcsI RAATTY 1 cut(s) 15
AcuI CTGAAG 2 cut(s) 269, 314
AfaI GTAC 5 cut(s) 319, 482, 530, 674, 836
AfiI CCNNNNNNNGG 5 cut(s) 289, 422, 449, 650, 797
AgsI TTSAA 6 cut(s) 20, 64, 110, 123, 200, 726
AjnI CCWGG 1 cut(s) 649
AluBI AGCT 7 cut(s) 28, 473, 551, 560, 566, 626, 692
AluI AGCT 7 cut(s) 28, 473, 551, 560, 566, 626, 692
Alw21I GWGCWC 1 cut(s) 457
Alw26I GTCTC 1 cut(s) 303
AlwI GGATC 1 cut(s) 86
AlwNI CAGNNNCTG 1 cut(s) 503
Ama87I CYCGRG 1 cut(s) 795
AoxI GGCC 2 cut(s) 22, 323
ApeKI GCWGC 4 cut(s) 419, 548, 626, 692
ApoI RAATTY 1 cut(s) 15
AspS9I GGNCC 1 cut(s) 800
AsuHPI GGTGA 2 cut(s) 272, 582
AvaI CYCGRG 1 cut(s) 795
AvaII GGWCC 1 cut(s) 800
BaeGI GKGCMC 1 cut(s) 682
BalI TGGCCA 1 cut(s) 325
BanI GGYRCC 1 cut(s) 677
Bbv12I GWGCWC 1 cut(s) 457
BbvI GCAGC 4 cut(s) 431, 560, 613, 679
BccI CCATC 6 cut(s) 308, 442, 577, 673, 727, 751
BciT130I CCWGG 1 cut(s) 651
BciVI GTATCC 1 cut(s) 642
BcoDI GTCTC 1 cut(s) 303
BfuAI ACCTGC 1 cut(s) 469
BfuI GTATCC 1 cut(s) 642
BisI GCNGC 6 cut(s) 86, 365, 420, 549, 627, 693
BlsI GCNGC 6 cut(s) 87, 366, 421, 550, 628, 694
Bme1390I CCNGG 1 cut(s) 651
Bme18I GGWCC 1 cut(s) 800
BmeT110I CYCGRG 1 cut(s) 795
BmgT120I GGNCC 1 cut(s) 800
BmiI GGNNCC 1 cut(s) 679
BmrFI CCNGG 1 cut(s) 651
BmsI GCATC 1 cut(s) 509
BpmI CTGGAG 3 cut(s) 375, 480, 546
Bpu10I CCTNAGC 1 cut(s) 206
BpuEI CTTGAG 1 cut(s) 53
BsaXI ACNNNNNCTCC 4 cut(s) 478, 508, 718, 748
Bsc4I CCNNNNNNNGG 5 cut(s) 289, 422, 449, 650, 797
Bse118I RCCGGY 1 cut(s) 415
BseBI CCWGG 1 cut(s) 651
BseGI GGATG 1 cut(s) 665
BseLI CCNNNNNNNGG 5 cut(s) 289, 422, 449, 650, 797
BseMII CTCAG 1 cut(s) 314
BseRI GAGGAG 2 cut(s) 726, 729
BseSI GKGCMC 1 cut(s) 682
BseXI GCAGC 4 cut(s) 431, 560, 613, 679
BseYI CCCAGC 1 cut(s) 556
BsgI GTGCAG 3 cut(s) 423, 447, 690
Bsh1236I CGCG 1 cut(s) 85
BshFI GGCC 2 cut(s) 24, 325
BshNI GGYRCC 1 cut(s) 677
BsiHKAI GWGCWC 1 cut(s) 457
BsiHKCI CYCGRG 1 cut(s) 795
BsiSI CCGG 1 cut(s) 416
BsiWI CGTACG 1 cut(s) 672
BslI CCNNNNNNNGG 5 cut(s) 289, 422, 449, 650, 797
BsmAI GTCTC 1 cut(s) 303
BsnI GGCC 2 cut(s) 24, 325
BsoBI CYCGRG 1 cut(s) 795
Bsp1286I GDGCHC 2 cut(s) 457, 682
Bsp143I GATC 1 cut(s) 91
BspACI CCGC 3 cut(s) 85, 364, 645
BspANI GGCC 2 cut(s) 24, 325
BspCNI CTCAG 1 cut(s) 313
BspFNI CGCG 1 cut(s) 85
BspLI GGNNCC 1 cut(s) 679
BspMI ACCTGC 1 cut(s) 469
BspPI GGATC 1 cut(s) 86
BspT107I GGYRCC 1 cut(s) 677
BsrFI RCCGGY 1 cut(s) 415
BssAI RCCGGY 1 cut(s) 415
BssMI GATC 1 cut(s) 91
Bst2UI CCWGG 1 cut(s) 651
Bst4CI ACNGT 4 cut(s) 43, 242, 677, 834
BstAPI GCANNNNNTGC 3 cut(s) 428, 461, 626
BstC8I GCNNGC 3 cut(s) 26, 424, 471
BstDEI CTNAG 2 cut(s) 206, 300
BstENI CCTNNNNNAGG 1 cut(s) 287
BstF5I GGATG 1 cut(s) 665
BstFNI CGCG 1 cut(s) 85
BstKTI GATC 1 cut(s) 94
BstMAI GTCTC 1 cut(s) 303
BstMBI GATC 1 cut(s) 91
BstMWI GCNNNNNNNGC 7 cut(s) 373, 428, 437, 461, 470, 557, 626
BstNI CCWGG 1 cut(s) 651
BstSCI CCNGG 1 cut(s) 649
BstSLI GKGCMC 1 cut(s) 682
BstUI CGCG 1 cut(s) 85
BstV1I GCAGC 4 cut(s) 431, 560, 613, 679
BstX2I RGATCY 1 cut(s) 91
BstYI RGATCY 1 cut(s) 91
BsuI GTATCC 1 cut(s) 642
BsuRI GGCC 2 cut(s) 24, 325
BtsCI GGATG 1 cut(s) 665
BtsI GCAGTG 1 cut(s) 464
BtsIMutI CAGTG 3 cut(s) 39, 247, 464
BveI ACCTGC 1 cut(s) 469
Cac8I GCNNGC 3 cut(s) 26, 424, 471
CaiI CAGNNNCTG 1 cut(s) 503
Cfr10I RCCGGY 1 cut(s) 415
Cfr13I GGNCC 1 cut(s) 800
Csp6I GTAC 5 cut(s) 318, 481, 529, 673, 835
CviQI GTAC 5 cut(s) 318, 481, 529, 673, 835
DdeI CTNAG 2 cut(s) 206, 300
DpnI GATC 1 cut(s) 93
DpnII GATC 1 cut(s) 91
EaeI YGGCCR 1 cut(s) 323
Eco32I GATATC 1 cut(s) 659
Eco47I GGWCC 1 cut(s) 800
Eco57I CTGAAG 2 cut(s) 269, 314
Eco88I CYCGRG 1 cut(s) 795
EcoNI CCTNNNNNAGG 1 cut(s) 287
EcoRI GAATTC 1 cut(s) 15
EcoRII CCWGG 1 cut(s) 649
EcoRV GATATC 1 cut(s) 659
EcoT22I ATGCAT 1 cut(s) 378
FalI AAGNNNNNCTT 6 cut(s) 12, 44, 267, 299, 351, 383
FauI CCCGC 1 cut(s) 652
FauNDI CATATG 2 cut(s) 250, 477
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 6 cut(s) 86, 365, 420, 549, 627, 693
FokI GGATG 1 cut(s) 652
Fsp4HI GCNGC 6 cut(s) 86, 365, 420, 549, 627, 693
GluI GCNGC 6 cut(s) 86, 365, 420, 549, 627, 693
GsaI CCCAGC 1 cut(s) 560
GsuI CTGGAG 3 cut(s) 375, 480, 546
HaeIII GGCC 2 cut(s) 24, 325
HapII CCGG 1 cut(s) 416
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 1 cut(s) 187
HpaII CCGG 1 cut(s) 416
HphI GGTGA 2 cut(s) 272, 582
Hpy166II GTNNAC 2 cut(s) 6, 280
Hpy188I TCNGA 2 cut(s) 288, 333
Hpy188III TCNNGA 3 cut(s) 12, 32, 229
Hpy8I GTNNAC 2 cut(s) 6, 280
HpyAV CCTTC 5 cut(s) 194, 283, 293, 591, 609
HpyCH4III ACNGT 4 cut(s) 43, 242, 677, 834
HpyCH4IV ACGT 1 cut(s) 99
HpyCH4V TGCA 9 cut(s) 54, 376, 401, 440, 464, 469, 512, 695, 707
HpyF10VI GCNNNNNNNGC 7 cut(s) 373, 428, 437, 461, 470, 557, 626
HpyF3I CTNAG 2 cut(s) 206, 300
HpySE526I ACGT 1 cut(s) 99
Kzo9I GATC 1 cut(s) 91
LmnI GCTCC 3 cut(s) 452, 565, 571
Lsp1109I GCAGC 4 cut(s) 431, 560, 613, 679
LweI GCATC 1 cut(s) 509
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 3 cut(s) 37, 244, 305
MalI GATC 1 cut(s) 93
MboI GATC 1 cut(s) 91
MboII GAAGA 1 cut(s) 714
MflI RGATCY 1 cut(s) 91
MhlI GDGCHC 2 cut(s) 457, 682
MlsI TGGCCA 1 cut(s) 325
MluCI AATT 3 cut(s) 15, 118, 195
MluNI TGGCCA 1 cut(s) 325
Mox20I TGGCCA 1 cut(s) 325
Mph1103I ATGCAT 1 cut(s) 378
MscI TGGCCA 1 cut(s) 325
MseI TTAA 2 cut(s) 194, 222
Msp20I TGGCCA 1 cut(s) 325
MspA1I CMGCKG 2 cut(s) 626, 692
MspI CCGG 1 cut(s) 416
MspR9I CCNGG 1 cut(s) 651
MvaI CCWGG 1 cut(s) 651
MvnI CGCG 1 cut(s) 85
MwoI GCNNNNNNNGC 7 cut(s) 373, 428, 437, 461, 470, 557, 626
NdeI CATATG 2 cut(s) 250, 477
NdeII GATC 1 cut(s) 91
NlaIV GGNNCC 1 cut(s) 679
NmuCI GTSAC 2 cut(s) 37, 244
NsiI ATGCAT 1 cut(s) 378
PaeR7I CTCGAG 1 cut(s) 795
PaqCI CACCTGC 1 cut(s) 469
PfeI GAWTC 1 cut(s) 187
Pfl23II CGTACG 1 cut(s) 672
PflMI CCANNNNNTGG 1 cut(s) 449
PkrI GCNGC 6 cut(s) 87, 366, 421, 550, 628, 694
PsiI TTATAA 1 cut(s) 371
Psp6I CCWGG 1 cut(s) 649
PspFI CCCAGC 1 cut(s) 556
PspGI CCWGG 1 cut(s) 649
PspLI CGTACG 1 cut(s) 672
PspN4I GGNNCC 1 cut(s) 679
PspPI GGNCC 1 cut(s) 800
PspXI VCTCGAGB 1 cut(s) 795
PstNI CAGNNNCTG 1 cut(s) 503
PsuI RGATCY 1 cut(s) 91
PvuII CAGCTG 2 cut(s) 626, 692
RsaI GTAC 5 cut(s) 319, 482, 530, 674, 836
RsaNI GTAC 5 cut(s) 318, 481, 529, 673, 835
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 2 cut(s) 194, 222
SatI GCNGC 6 cut(s) 86, 365, 420, 549, 627, 693
Sau3AI GATC 1 cut(s) 91
Sau96I GGNCC 1 cut(s) 800
ScrFI CCNGG 1 cut(s) 651
SduI GDGCHC 2 cut(s) 457, 682
SfaNI GCATC 1 cut(s) 509
Sfr274I CTCGAG 1 cut(s) 795
SinI GGWCC 1 cut(s) 800
SlaI CTCGAG 1 cut(s) 795
SmlI CTYRAG 2 cut(s) 32, 795
SmoI CTYRAG 2 cut(s) 32, 795
Sse9I AATT 3 cut(s) 15, 118, 195
SsiI CCGC 3 cut(s) 85, 364, 645
StyD4I CCNGG 1 cut(s) 649
TaaI ACNGT 4 cut(s) 43, 242, 677, 834
TaiI ACGT 1 cut(s) 102
TaqI TCGA 3 cut(s) 5, 383, 796
TasI AATT 3 cut(s) 15, 118, 195
TatI WGTACW 1 cut(s) 480
TauI GCSGC 2 cut(s) 88, 367
TfiI GAWTC 1 cut(s) 187
Tru1I TTAA 2 cut(s) 194, 222
Tru9I TTAA 2 cut(s) 194, 222
TscAI CASTG 3 cut(s) 46, 247, 471
TseFI GTSAC 2 cut(s) 37, 244
TseI GCWGC 4 cut(s) 419, 548, 626, 692
Tsp45I GTSAC 2 cut(s) 37, 244
TspDTI ATGAA 2 cut(s) 230, 591
TspGWI ACGGA 2 cut(s) 165, 792
TspRI CASTG 3 cut(s) 46, 247, 471
Van91I CCANNNNNTGG 1 cut(s) 449
VpaK11BI GGWCC 1 cut(s) 800
XagI CCTNNNNNAGG 1 cut(s) 287
XapI RAATTY 1 cut(s) 15
XhoI CTCGAG 1 cut(s) 795
XmiI GTMKAC 1 cut(s) 5
Zsp2I ATGCAT 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.