MD00G1045300.v1.1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0011916
Physical Location & Seq
Reverse (-)
8300193 .. 8303425
3233 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1045300.v1.1.491

Sequence Viewer

Length: 822 bp
ATGTCAAGATTTGAACTTTCATCATCCTATTCGTCTGCACCATACTACTCATCGTTGCCTCCTGACTATTCCTTCATGTCGATTTCTGGAATTCAAGGCCAGCTTCTTGAAGTTACTGTTGTTGGTTGTAACAAATTGAGAGATACGGAATGGATTTCTCGGCAGGACCCATATGTCTGTCTTGAATATGCTCACAACAAGCACCGCACCAGAACATGCACAGATGGTGGTAAAAATCCCACATTCCAAGAGAAGTTCGTGTTTCCGCTGATTGAAGGTCTTAGGGAACTCAATGTTCTGGTCTGGAACAGCAATACTGTCACATTGGACGACTTCATTGGCGAGGGAAAGGTTCAACTTCAGAAGGTTCTTACTCAGGGTTATGACGACAGCACATGGCAGCTTCAGACTAAACATGGCCGATATGGAGGAGAAGTGCAGCTCATAATGCATTATTCGAAGACAAATAAACCCGCATCAAGCTATGCTCCATCTGCACCACCATATGGAGCACCACATGCATCACGAGCTTATCTATATTCAACCCCCCCACCTGCAGGTCATTATGCACCACCAGCGCCTGGACCTTACCCACCAGCGCCAGCAGGGTACCCAGCTCCATCTCCCTATCCTTTGTACTCGCCTAATACAGCAGGGTATCCACCATCTGCATATGGTCCCCCTCCGCCAGCGTATGGCCATACTCCGTCTGCCTATCCTCCCCCACCATACCCACCAACTTCAACATATCCCCCACTCCCGCAATCCTCACCCTATCCTCCAAGTCCTTTCCCTGGACTTTATCCTCCACCACCGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04620 map04620
Pfam Domains
Protein Families
C2

Protein Analysis

274

Amino Acids

29.93

Weight (kDa)

7.04

Isoelectric Point (pI)

77.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 35 - 133 1.3e-15 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011916)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34150
fragaria_vesca FvH4_2g05480
malus_domestica MD00G1045300.v1.1 MD05G1087200.v1.1
prunus_persica Prupe.8G131800_v2.0.a1 Prupe.8G131800_v2.0.a1
pyrus_communis pycom05g08650 pycom10g08200
rosa_chinensis RchiOBHm_Chr6g0255751
rosa_laevigata RLG00000014816
rosa_multiflora Rmu_sc0001163.1_g000008 Rmu_sc0001163.1_g000009
rosa_roxburghii Rroxscaffold_7G00209700
rosa_rugosa Rorug05G0561000 Rorug05G0561100
rosa_samantha Rh6BG072200 Rh6CG070400 Rh6DG067600
rosa_wichuraiana Rw6G006820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 562
AasI GACNNNNNNGTC 1 cut(s) 173
Acc36I ACCTGC 2 cut(s) 548, 562
Acc65I GGTACC 1 cut(s) 609
AccB1I GGYRCC 1 cut(s) 609
AccB7I CCANNNNNTGG 3 cut(s) 506, 581, 695
AciI CCGC 5 cut(s) 205, 266, 474, 686, 761
AcoI YGGCCR 2 cut(s) 418, 697
AcsI RAATTY 1 cut(s) 90
AcuI CTGAAG 2 cut(s) 344, 389
AfaI GTAC 3 cut(s) 611, 638, 818
AfiI CCNNNNNNNGG 5 cut(s) 506, 557, 581, 695, 794
AgsI TTSAA 8 cut(s) 14, 95, 110, 185, 275, 356, 543, 744
AjnI CCWGG 2 cut(s) 580, 793
AluBI AGCT 6 cut(s) 103, 403, 442, 483, 530, 617
AluI AGCT 6 cut(s) 103, 403, 442, 483, 530, 617
Alw21I GWGCWC 1 cut(s) 514
AlwNI CAGNNNCTG 1 cut(s) 581
AoxI GGCC 3 cut(s) 97, 418, 697
ApeKI GCWGC 2 cut(s) 400, 439
ApoI RAATTY 1 cut(s) 90
Asp718I GGTACC 1 cut(s) 609
AspLEI GCGC 2 cut(s) 580, 601
AspS9I GGNCC 3 cut(s) 166, 584, 677
AsuHPI GGTGA 1 cut(s) 762
AsuII TTCGAA 1 cut(s) 458
AvaII GGWCC 3 cut(s) 166, 584, 677
BalI TGGCCA 1 cut(s) 699
BanI GGYRCC 1 cut(s) 609
BauI CACGAG 1 cut(s) 525
BbsI GAAGAC 1 cut(s) 467
Bbv12I GWGCWC 1 cut(s) 514
BbvI GCAGC 2 cut(s) 412, 451
BccI CCATC 4 cut(s) 218, 499, 628, 673
BciT130I CCWGG 2 cut(s) 582, 795
BciVI GTATCC 1 cut(s) 669
BfmI CTRYAG 1 cut(s) 555
BfoI RGCGCY 2 cut(s) 581, 602
BfuAI ACCTGC 2 cut(s) 548, 562
BfuI GTATCC 1 cut(s) 669
BisI GCNGC 2 cut(s) 401, 440
BlsI GCNGC 2 cut(s) 402, 441
Bme1390I CCNGG 2 cut(s) 582, 795
Bme18I GGWCC 3 cut(s) 166, 584, 677
BmgT120I GGNCC 3 cut(s) 166, 584, 677
BmiI GGNNCC 3 cut(s) 168, 611, 679
BmrFI CCNGG 2 cut(s) 582, 795
BmsI GCATC 2 cut(s) 485, 530
BpiI GAAGAC 1 cut(s) 467
Bpu14I TTCGAA 1 cut(s) 458
BsaJI CCNNGG 1 cut(s) 793
BsaXI ACNNNNNCTCC 2 cut(s) 420, 450
Bsc4I CCNNNNNNNGG 5 cut(s) 506, 557, 581, 695, 794
BseBI CCWGG 2 cut(s) 582, 795
BseDI CCNNGG 1 cut(s) 793
BseGI GGATG 1 cut(s) 23
BseLI CCNNNNNNNGG 5 cut(s) 506, 557, 581, 695, 794
BseMII CTCAG 1 cut(s) 389
BseRI GAGGAG 1 cut(s) 444
BseXI GCAGC 2 cut(s) 412, 451
BseYI CCCAGC 1 cut(s) 613
BsgI GTGCAG 3 cut(s) 21, 458, 480
BshFI GGCC 3 cut(s) 99, 420, 699
BshNI GGYRCC 1 cut(s) 609
BsiHKAI GWGCWC 1 cut(s) 514
BslFI GGGAC 1 cut(s) 663
BslI CCNNNNNNNGG 5 cut(s) 506, 557, 581, 695, 794
BsmFI GGGAC 1 cut(s) 663
BsnI GGCC 3 cut(s) 99, 420, 699
Bsp119I TTCGAA 1 cut(s) 458
Bsp1286I GDGCHC 1 cut(s) 514
BspACI CCGC 5 cut(s) 205, 266, 474, 686, 761
BspANI GGCC 3 cut(s) 99, 420, 699
BspCNI CTCAG 1 cut(s) 388
BspLI GGNNCC 3 cut(s) 168, 611, 679
BspMAI CTGCAG 1 cut(s) 559
BspMI ACCTGC 2 cut(s) 548, 562
BspT104I TTCGAA 1 cut(s) 458
BspT107I GGYRCC 1 cut(s) 609
BssECI CCNNGG 1 cut(s) 793
BssSI CACGAG 1 cut(s) 525
Bst2BI CACGAG 1 cut(s) 525
Bst2UI CCWGG 2 cut(s) 582, 795
Bst4CI ACNGT 3 cut(s) 118, 319, 816
BstAPI GCANNNNNTGC 1 cut(s) 518
BstBI TTCGAA 1 cut(s) 458
BstC8I GCNNGC 3 cut(s) 101, 603, 690
BstDEI CTNAG 2 cut(s) 281, 375
BstF5I GGATG 1 cut(s) 23
BstH2I RGCGCY 2 cut(s) 581, 602
BstHHI GCGC 2 cut(s) 580, 601
BstMWI GCNNNNNNNGC 5 cut(s) 448, 494, 518, 527, 575
BstNI CCWGG 2 cut(s) 582, 795
BstNSI RCATGY 2 cut(s) 219, 521
BstSCI CCNGG 2 cut(s) 580, 793
BstSFI CTRYAG 1 cut(s) 555
BstV1I GCAGC 2 cut(s) 412, 451
BstV2I GAAGAC 1 cut(s) 467
BsuI GTATCC 1 cut(s) 669
BsuRI GGCC 3 cut(s) 99, 420, 699
BtsCI GGATG 1 cut(s) 23
BveI ACCTGC 2 cut(s) 548, 562
Cac8I GCNNGC 3 cut(s) 101, 603, 690
CaiI CAGNNNCTG 1 cut(s) 581
CfoI GCGC 2 cut(s) 580, 601
Cfr13I GGNCC 3 cut(s) 166, 584, 677
Csp6I GTAC 3 cut(s) 610, 637, 817
CviAII CATG 5 cut(s) 76, 216, 396, 416, 518
CviJI RGCY 9 cut(s) 99, 103, 403, 420, 442, 483, 530, 617, 699
CviKI_1 RGCY 9 cut(s) 99, 103, 403, 420, 442, 483, 530, 617, 699
CviQI GTAC 3 cut(s) 610, 637, 817
DdeI CTNAG 2 cut(s) 281, 375
DrdI GACNNNNNNGTC 1 cut(s) 173
DseDI GACNNNNNNGTC 1 cut(s) 173
EaeI YGGCCR 2 cut(s) 418, 697
EciI GGCGGA 1 cut(s) 675
Eco47I GGWCC 3 cut(s) 166, 584, 677
Eco57I CTGAAG 2 cut(s) 344, 389
EcoO109I RGGNCCY 1 cut(s) 166
EcoRI GAATTC 1 cut(s) 90
EcoRII CCWGG 2 cut(s) 580, 793
EcoT22I ATGCAT 2 cut(s) 453, 523
FaeI CATG 5 cut(s) 79, 219, 399, 419, 521
FalI AAGNNNNNCTT 2 cut(s) 87, 119
FaqI GGGAC 1 cut(s) 663
FatI CATG 5 cut(s) 75, 215, 395, 415, 517
FauI CCCGC 2 cut(s) 481, 768
FauNDI CATATG 3 cut(s) 172, 505, 673
Fnu4HI GCNGC 2 cut(s) 401, 440
FokI GGATG 1 cut(s) 10
Fsp4HI GCNGC 2 cut(s) 401, 440
GlaI GCGC 2 cut(s) 579, 600
GluI GCNGC 2 cut(s) 401, 440
GsaI CCCAGC 1 cut(s) 617
HaeII RGCGCY 2 cut(s) 581, 602
HaeIII GGCC 3 cut(s) 99, 420, 699
HhaI GCGC 2 cut(s) 580, 601
Hin1II CATG 5 cut(s) 79, 219, 399, 419, 521
Hin6I GCGC 2 cut(s) 578, 599
HinP1I GCGC 2 cut(s) 578, 599
HphI GGTGA 1 cut(s) 762
Hpy188I TCNGA 2 cut(s) 363, 408
Hpy188III TCNNGA 7 cut(s) 6, 62, 87, 107, 182, 304, 525
HpyAV CCTTC 3 cut(s) 82, 269, 358
HpyCH4III ACNGT 3 cut(s) 118, 319, 816
HpyCH4V TGCA 9 cut(s) 38, 219, 439, 451, 497, 521, 557, 569, 671
HpyF10VI GCNNNNNNNGC 5 cut(s) 448, 494, 518, 527, 575
HpyF3I CTNAG 2 cut(s) 281, 375
Hsp92II CATG 5 cut(s) 79, 219, 399, 419, 521
HspAI GCGC 2 cut(s) 578, 599
KpnI GGTACC 1 cut(s) 613
LmnI GCTCC 3 cut(s) 493, 509, 622
Lsp1109I GCAGC 2 cut(s) 412, 451
LweI GCATC 2 cut(s) 485, 530
MaeIII GTNAC 3 cut(s) 112, 128, 319
MboII GAAGA 1 cut(s) 472
MhlI GDGCHC 1 cut(s) 514
MlsI TGGCCA 1 cut(s) 699
MluCI AATT 2 cut(s) 90, 134
MluNI TGGCCA 1 cut(s) 699
MnlI CCTC 8 cut(s) 69, 337, 422, 693, 729, 778, 789, 816
Mox20I TGGCCA 1 cut(s) 699
Mph1103I ATGCAT 2 cut(s) 453, 523
MscI TGGCCA 1 cut(s) 699
Msp20I TGGCCA 1 cut(s) 699
MspA1I CMGCKG 1 cut(s) 268
MspR9I CCNGG 2 cut(s) 582, 795
MvaI CCWGG 2 cut(s) 582, 795
MwoI GCNNNNNNNGC 5 cut(s) 448, 494, 518, 527, 575
NdeI CATATG 3 cut(s) 172, 505, 673
NlaIII CATG 5 cut(s) 79, 219, 399, 419, 521
NlaIV GGNNCC 3 cut(s) 168, 611, 679
NmeAIII GCCGAG 1 cut(s) 139
NmuCI GTSAC 1 cut(s) 319
NsiI ATGCAT 2 cut(s) 453, 523
NspI RCATGY 2 cut(s) 219, 521
NspV TTCGAA 1 cut(s) 458
PaqCI CACCTGC 1 cut(s) 562
PflMI CCANNNNNTGG 3 cut(s) 506, 581, 695
PkrI GCNGC 2 cut(s) 402, 441
PpuMI RGGWCCY 1 cut(s) 166
Psp5II RGGWCCY 1 cut(s) 166
Psp6I CCWGG 2 cut(s) 580, 793
PspFI CCCAGC 1 cut(s) 613
PspGI CCWGG 2 cut(s) 580, 793
PspN4I GGNNCC 3 cut(s) 168, 611, 679
PspPI GGNCC 3 cut(s) 166, 584, 677
PspPPI RGGWCCY 1 cut(s) 166
PstI CTGCAG 1 cut(s) 559
PstNI CAGNNNCTG 1 cut(s) 581
RsaI GTAC 3 cut(s) 611, 638, 818
RsaNI GTAC 3 cut(s) 610, 637, 817
SatI GCNGC 2 cut(s) 401, 440
Sau96I GGNCC 3 cut(s) 166, 584, 677
SbfI CCTGCAGG 1 cut(s) 559
ScrFI CCNGG 2 cut(s) 582, 795
SdaI CCTGCAGG 1 cut(s) 559
SduI GDGCHC 1 cut(s) 514
SfaNI GCATC 2 cut(s) 485, 530
SfcI CTRYAG 1 cut(s) 555
SfuI TTCGAA 1 cut(s) 458
SinI GGWCC 3 cut(s) 166, 584, 677
Sse8387I CCTGCAGG 1 cut(s) 559
Sse9I AATT 2 cut(s) 90, 134
SsiI CCGC 5 cut(s) 205, 266, 474, 686, 761
StyD4I CCNGG 2 cut(s) 580, 793
TaaI ACNGT 3 cut(s) 118, 319, 816
TaqI TCGA 2 cut(s) 80, 458
TasI AATT 2 cut(s) 90, 134
TatI WGTACW 1 cut(s) 636
TseFI GTSAC 1 cut(s) 319
TseI GCWGC 2 cut(s) 400, 439
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 3 cut(s) 9, 64, 325
TspGWI ACGGA 2 cut(s) 161, 696
Van91I CCANNNNNTGG 3 cut(s) 506, 581, 695
VpaK11BI GGWCC 3 cut(s) 166, 584, 677
XapI RAATTY 1 cut(s) 90
XceI RCATGY 2 cut(s) 219, 521
Zsp2I ATGCAT 2 cut(s) 453, 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.