MD05G1087200.v1.1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011916
Physical Location & Seq
Forward (+)
18585041 .. 18588135
3095 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1087200.v1.1.491

Sequence Viewer

Length: 747 bp
ATGTCGATTTCTGGAATTCAAGGCCAGCTTCTTGAAGTCACTGTTATTGGTTGTAACAAATTGAGAGATACGGAATGGATTTCACGGCAGGACCCATATGTCTGTCTTGAATATGCTAACACCAAGCACCGCACCAGAACATGCACAGACGGCGGTAAACATCCCACATTCCAAGAGAAGTTCGTGTTTCCACTGGTTGAAGGTCTTAGGGAAATCAATGTTATGGTCTGGAACAGCAACACTGTCACATCGGACGACTTCATTGGCGGGGGAAAGGTTCAACTTCAGAAGGTTCTTACTCAGGATTATGACGACAGCGCATGGCCGCTTCAGACTAAACATGGCCGATATGCAGGAGAAGTGCGGCTCATAATGCATTATTCGAAGACCAATAAACCGACATCAAGCTATGCTCCGTCTGCAGCACCATATGGAGCACCACATGCATCACAAGCTCATCTATATTCAGCCCCCCCACCTGCAGGTCATTATGCATCACCAGCGCCTGGACCTTACCCACCAGTGCCAGCAGGCTACCCAGCTCCATCTCCCTATCCTTCATACTTGCCTAATTCAGCAGGGTATCCACCATCTCCATATGGTCCCAATCCATCGGCGTATGGCCATACTCCATCTTCCTATCCTCTCGCACCATACCCACCAACTTCAACATATCCCCCACCCCCACAATCCTCACCCTATCCTCCAAATCCTTTTCCTGGACTTTATCCTCCATCACCGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04620 map04620
Pfam Domains
Protein Families
C2

Protein Analysis

249

Amino Acids

26.99

Weight (kDa)

7.72

Isoelectric Point (pI)

74.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 10 - 101 3.3e-15 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011916)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34150
fragaria_vesca FvH4_2g05480
malus_domestica MD00G1045300.v1.1 MD05G1087200.v1.1
prunus_persica Prupe.8G131800_v2.0.a1 Prupe.8G131800_v2.0.a1
pyrus_communis pycom05g08650 pycom10g08200
rosa_chinensis RchiOBHm_Chr6g0255751
rosa_laevigata RLG00000014816
rosa_multiflora Rmu_sc0001163.1_g000008 Rmu_sc0001163.1_g000009
rosa_roxburghii Rroxscaffold_7G00209700
rosa_rugosa Rorug05G0561000 Rorug05G0561100
rosa_samantha Rh6BG072200 Rh6CG070400 Rh6DG067600
rosa_wichuraiana Rw6G006820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 487
AasI GACNNNNNNGTC 1 cut(s) 98
Acc36I ACCTGC 2 cut(s) 473, 487
AccB7I CCANNNNNTGG 1 cut(s) 506
AciI CCGC 5 cut(s) 130, 153, 267, 326, 364
AcoI YGGCCR 3 cut(s) 323, 343, 622
AcsI RAATTY 1 cut(s) 15
AcuI CTGAAG 2 cut(s) 269, 314
AfaI GTAC 1 cut(s) 743
AfiI CCNNNNNNNGG 3 cut(s) 482, 506, 719
AgsI TTSAA 6 cut(s) 20, 35, 110, 200, 281, 669
AjnI CCWGG 2 cut(s) 505, 718
AluBI AGCT 4 cut(s) 28, 408, 455, 542
AluI AGCT 4 cut(s) 28, 408, 455, 542
Alw21I GWGCWC 1 cut(s) 439
AlwNI CAGNNNCTG 1 cut(s) 506
AoxI GGCC 4 cut(s) 22, 323, 343, 622
ApeKI GCWGC 1 cut(s) 422
ApoI RAATTY 1 cut(s) 15
AspLEI GCGC 2 cut(s) 320, 505
AspS9I GGNCC 3 cut(s) 91, 509, 602
AsuHPI GGTGA 3 cut(s) 489, 687, 729
AsuII TTCGAA 1 cut(s) 383
AvaII GGWCC 3 cut(s) 91, 509, 602
BalI TGGCCA 1 cut(s) 624
BbsI GAAGAC 1 cut(s) 392
Bbv12I GWGCWC 1 cut(s) 439
BbvI GCAGC 1 cut(s) 434
BccI CCATC 5 cut(s) 553, 598, 619, 640, 742
BceAI ACGGC 2 cut(s) 101, 166
BciT130I CCWGG 2 cut(s) 507, 720
BciVI GTATCC 1 cut(s) 594
BfmI CTRYAG 2 cut(s) 420, 480
BfoI RGCGCY 1 cut(s) 506
BfuAI ACCTGC 2 cut(s) 473, 487
BfuI GTATCC 1 cut(s) 594
BisI GCNGC 3 cut(s) 326, 365, 423
BlsI GCNGC 3 cut(s) 327, 366, 424
Bme1390I CCNGG 2 cut(s) 507, 720
Bme18I GGWCC 3 cut(s) 91, 509, 602
BmgT120I GGNCC 3 cut(s) 91, 509, 602
BmiI GGNNCC 2 cut(s) 93, 604
BmrFI CCNGG 2 cut(s) 507, 720
BmsI GCATC 2 cut(s) 455, 503
BpiI GAAGAC 1 cut(s) 392
Bpu14I TTCGAA 1 cut(s) 383
Bsc4I CCNNNNNNNGG 3 cut(s) 482, 506, 719
Bse1I ACTGG 2 cut(s) 198, 521
BseBI CCWGG 2 cut(s) 507, 720
BseGI GGATG 1 cut(s) 160
BseLI CCNNNNNNNGG 3 cut(s) 482, 506, 719
BseMII CTCAG 1 cut(s) 314
BseNI ACTGG 2 cut(s) 198, 521
BseXI GCAGC 1 cut(s) 434
BseYI CCCAGC 1 cut(s) 538
BshFI GGCC 4 cut(s) 24, 325, 345, 624
BsiHKAI GWGCWC 1 cut(s) 439
BslFI GGGAC 1 cut(s) 588
BslI CCNNNNNNNGG 3 cut(s) 482, 506, 719
BsmFI GGGAC 1 cut(s) 588
BsnI GGCC 4 cut(s) 24, 325, 345, 624
Bsp119I TTCGAA 1 cut(s) 383
Bsp1286I GDGCHC 1 cut(s) 439
BspACI CCGC 5 cut(s) 130, 153, 267, 326, 364
BspANI GGCC 4 cut(s) 24, 325, 345, 624
BspCNI CTCAG 1 cut(s) 313
BspLI GGNNCC 2 cut(s) 93, 604
BspMAI CTGCAG 2 cut(s) 424, 484
BspMI ACCTGC 2 cut(s) 473, 487
BspT104I TTCGAA 1 cut(s) 383
BsrI ACTGG 2 cut(s) 198, 521
Bst2UI CCWGG 2 cut(s) 507, 720
Bst4CI ACNGT 3 cut(s) 43, 244, 741
BstAPI GCANNNNNTGC 1 cut(s) 443
BstBI TTCGAA 1 cut(s) 383
BstC8I GCNNGC 3 cut(s) 26, 528, 532
BstDEI CTNAG 2 cut(s) 206, 300
BstF5I GGATG 1 cut(s) 160
BstH2I RGCGCY 1 cut(s) 506
BstHHI GCGC 2 cut(s) 320, 505
BstMWI GCNNNNNNNGC 6 cut(s) 150, 373, 419, 443, 452, 500
BstNI CCWGG 2 cut(s) 507, 720
BstNSI RCATGY 2 cut(s) 144, 446
BstSCI CCNGG 2 cut(s) 505, 718
BstSFI CTRYAG 2 cut(s) 420, 480
BstV1I GCAGC 1 cut(s) 434
BstV2I GAAGAC 1 cut(s) 392
BsuI GTATCC 1 cut(s) 594
BsuRI GGCC 4 cut(s) 24, 325, 345, 624
BtsCI GGATG 1 cut(s) 160
BtsIMutI CAGTG 4 cut(s) 39, 191, 240, 528
BveI ACCTGC 2 cut(s) 473, 487
Cac8I GCNNGC 3 cut(s) 26, 528, 532
CaiI CAGNNNCTG 1 cut(s) 506
CfoI GCGC 2 cut(s) 320, 505
Cfr13I GGNCC 3 cut(s) 91, 509, 602
Csp6I GTAC 1 cut(s) 742
CviAII CATG 4 cut(s) 141, 321, 341, 443
CviQI GTAC 1 cut(s) 742
DdeI CTNAG 2 cut(s) 206, 300
DrdI GACNNNNNNGTC 1 cut(s) 98
DseDI GACNNNNNNGTC 1 cut(s) 98
EaeI YGGCCR 3 cut(s) 323, 343, 622
Eco47I GGWCC 3 cut(s) 91, 509, 602
Eco57I CTGAAG 2 cut(s) 269, 314
EcoO109I RGGNCCY 1 cut(s) 91
EcoRI GAATTC 1 cut(s) 15
EcoRII CCWGG 2 cut(s) 505, 718
EcoT22I ATGCAT 3 cut(s) 378, 448, 496
FaeI CATG 4 cut(s) 144, 324, 344, 446
FalI AAGNNNNNCTT 2 cut(s) 12, 44
FaqI GGGAC 1 cut(s) 588
FatI CATG 4 cut(s) 140, 320, 340, 442
FauI CCCGC 1 cut(s) 260
FauNDI CATATG 3 cut(s) 97, 430, 598
Fnu4HI GCNGC 3 cut(s) 326, 365, 423
FokI GGATG 1 cut(s) 147
Fsp4HI GCNGC 3 cut(s) 326, 365, 423
GlaI GCGC 2 cut(s) 319, 504
GluI GCNGC 3 cut(s) 326, 365, 423
GsaI CCCAGC 1 cut(s) 542
HaeII RGCGCY 1 cut(s) 506
HaeIII GGCC 4 cut(s) 24, 325, 345, 624
HhaI GCGC 2 cut(s) 320, 505
Hin1II CATG 4 cut(s) 144, 324, 344, 446
Hin6I GCGC 2 cut(s) 318, 503
HinP1I GCGC 2 cut(s) 318, 503
HphI GGTGA 3 cut(s) 489, 687, 729
Hpy166II GTNNAC 1 cut(s) 158
Hpy188I TCNGA 3 cut(s) 253, 288, 333
Hpy188III TCNNGA 5 cut(s) 12, 32, 107, 229, 302
Hpy8I GTNNAC 1 cut(s) 158
HpyAV CCTTC 3 cut(s) 194, 283, 567
HpyCH4III ACNGT 3 cut(s) 43, 244, 741
HpyCH4V TGCA 7 cut(s) 144, 353, 376, 422, 446, 482, 494
HpyF10VI GCNNNNNNNGC 6 cut(s) 150, 373, 419, 443, 452, 500
HpyF3I CTNAG 2 cut(s) 206, 300
Hsp92II CATG 4 cut(s) 144, 324, 344, 446
HspAI GCGC 2 cut(s) 318, 503
LmnI GCTCC 3 cut(s) 418, 434, 547
Lsp1109I GCAGC 1 cut(s) 434
LweI GCATC 2 cut(s) 455, 503
MaeIII GTNAC 3 cut(s) 37, 53, 244
MboII GAAGA 2 cut(s) 397, 627
MhlI GDGCHC 1 cut(s) 439
MlsI TGGCCA 1 cut(s) 624
MluCI AATT 3 cut(s) 15, 59, 571
MluNI TGGCCA 1 cut(s) 624
MnlI CCTC 4 cut(s) 654, 703, 714, 741
Mox20I TGGCCA 1 cut(s) 624
Mph1103I ATGCAT 3 cut(s) 378, 448, 496
MscI TGGCCA 1 cut(s) 624
Msp20I TGGCCA 1 cut(s) 624
MspR9I CCNGG 2 cut(s) 507, 720
MvaI CCWGG 2 cut(s) 507, 720
MwoI GCNNNNNNNGC 6 cut(s) 150, 373, 419, 443, 452, 500
NdeI CATATG 3 cut(s) 97, 430, 598
NlaIII CATG 4 cut(s) 144, 324, 344, 446
NlaIV GGNNCC 2 cut(s) 93, 604
NmuCI GTSAC 2 cut(s) 37, 244
NsiI ATGCAT 3 cut(s) 378, 448, 496
NspI RCATGY 2 cut(s) 144, 446
NspV TTCGAA 1 cut(s) 383
PaqCI CACCTGC 1 cut(s) 487
PflMI CCANNNNNTGG 1 cut(s) 506
PfoI TCCNGGA 1 cut(s) 718
PkrI GCNGC 3 cut(s) 327, 366, 424
PpuMI RGGWCCY 1 cut(s) 91
Psp5II RGGWCCY 1 cut(s) 91
Psp6I CCWGG 2 cut(s) 505, 718
PspFI CCCAGC 1 cut(s) 538
PspGI CCWGG 2 cut(s) 505, 718
PspN4I GGNNCC 2 cut(s) 93, 604
PspPI GGNCC 3 cut(s) 91, 509, 602
PspPPI RGGWCCY 1 cut(s) 91
PstI CTGCAG 2 cut(s) 424, 484
PstNI CAGNNNCTG 1 cut(s) 506
RsaI GTAC 1 cut(s) 743
RsaNI GTAC 1 cut(s) 742
SatI GCNGC 3 cut(s) 326, 365, 423
Sau96I GGNCC 3 cut(s) 91, 509, 602
SbfI CCTGCAGG 1 cut(s) 484
ScrFI CCNGG 2 cut(s) 507, 720
SdaI CCTGCAGG 1 cut(s) 484
SduI GDGCHC 1 cut(s) 439
SfaNI GCATC 2 cut(s) 455, 503
SfcI CTRYAG 2 cut(s) 420, 480
SfuI TTCGAA 1 cut(s) 383
SinI GGWCC 3 cut(s) 91, 509, 602
Sse8387I CCTGCAGG 1 cut(s) 484
Sse9I AATT 3 cut(s) 15, 59, 571
SsiI CCGC 5 cut(s) 130, 153, 267, 326, 364
StyD4I CCNGG 2 cut(s) 505, 718
TaaI ACNGT 3 cut(s) 43, 244, 741
TaqI TCGA 2 cut(s) 5, 383
TasI AATT 3 cut(s) 15, 59, 571
TauI GCSGC 2 cut(s) 328, 367
TscAI CASTG 4 cut(s) 46, 198, 247, 528
TseFI GTSAC 2 cut(s) 37, 244
TseI GCWGC 1 cut(s) 422
Tsp45I GTSAC 2 cut(s) 37, 244
TspDTI ATGAA 2 cut(s) 250, 549
TspGWI ACGGA 2 cut(s) 86, 405
TspRI CASTG 4 cut(s) 46, 198, 247, 528
Van91I CCANNNNNTGG 1 cut(s) 506
VpaK11BI GGWCC 3 cut(s) 91, 509, 602
XapI RAATTY 1 cut(s) 15
XceI RCATGY 2 cut(s) 144, 446
Zsp2I ATGCAT 3 cut(s) 378, 448, 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.