Rw6G006820

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0011916
Physical Location & Seq
Reverse (-)
10912549 .. 10915440
2892 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G006820.1

Sequence Viewer

Length: 963 bp
ATGTCATATTATCAATTTCCAACCCAGTCTTCTTCGTCTTCGTCTCCGTCTCCTTACTCCTCTTTTCGCATGTCGACTTCTGGTATTCAAGGCCAGCTTCTTGAGGTCACTGTTGTTGGGTGCAATAAGTTGAAAGATACAGAATGGATTTCGCGGCAAGATCCCTACGTTTGTGTTGAATATGCTAACTCCAAGCACAGAACAAGAACGCATACTGGTTCAAATCCGAATTTGGACTCGAACTGGACTGAAAGCCATGTTGATTTTAATGGTGTAGACGGAGGTAGAAACCCTACATTCCAAGAGAAGTTCGTGATTCCTCTAATTGAAGGGCTTAGGGAGATGAATGTTGCTGTCTGGAACAGCAACAGTGTCACATATGACGACTTTATTGGCGAGGGAAAAGTACACCTTCAGAAGGTTCTGTCTCAGGGTTACGATGATGGTACTTGGCCACTTCAGACTAAAACGGGCAGATATGCTGGAGAAGTGCGGCTTATAATGCATTACTCGAAGCCTGATATAAGTGCAAAGCCTAATAAACCGGCAGCAGGCTATGCCCCATCTGCACCACCTTATGGAGCACCACCTGCACCGCAAGCTCATATGTACTCAACTCCCCCTCCAGCATCTGCGTATGCACCACCACCTCAAATGTATGCACCACCTCCACCAGCAGCTTACCCAGCTCCAGCTCCATCTCCCTATCCTTCGTATTCACCTACTCCTTCATATCCACCCAATCCAGCACCAGCTGCGTATCCACCTAATCCCGCACCAGGAAGATATCCACCATCCCCGTACGGTGCCCCTCCACCAGCTGCATATCCTCCTGCACCATACCCACCATCTTCAACTTATCCTCCTCCTCCATCATCTACATATCCACCACCTCCACAAGCCTCATCGTTTTACCCTCCACCTCAAGGTCCATATGGAGTATATCCACCACCACCATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04620 map04620
Pfam Domains
Protein Families
C2

Protein Analysis

320

Amino Acids

34.59

Weight (kDa)

7.0

Isoelectric Point (pI)

79.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 94 - 145 6e-06 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011916)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34150
fragaria_vesca FvH4_2g05480
malus_domestica MD00G1045300.v1.1 MD05G1087200.v1.1
prunus_persica Prupe.8G131800_v2.0.a1 Prupe.8G131800_v2.0.a1
pyrus_communis pycom05g08650 pycom10g08200
rosa_chinensis RchiOBHm_Chr6g0255751
rosa_laevigata RLG00000014816
rosa_multiflora Rmu_sc0001163.1_g000008 Rmu_sc0001163.1_g000009
rosa_roxburghii Rroxscaffold_7G00209700
rosa_rugosa Rorug05G0561000 Rorug05G0561100
rosa_samantha Rh6BG072200 Rh6CG070400 Rh6DG067600
rosa_wichuraiana Rw6G006820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 500
AarI CACCTGC 1 cut(s) 598
Acc36I ACCTGC 1 cut(s) 598
AccB1I GGYRCC 1 cut(s) 806
AccB7I CCANNNNNTGG 1 cut(s) 578
AccI GTMKAC 2 cut(s) 74, 276
AccII CGCG 1 cut(s) 154
AciI CCGC 4 cut(s) 154, 493, 596, 774
AclWI GGATC 1 cut(s) 155
AcoI YGGCCR 1 cut(s) 452
AcsI RAATTY 1 cut(s) 229
AcuI CTGAAG 2 cut(s) 398, 443
AfaI GTAC 4 cut(s) 408, 448, 611, 803
AfiI CCNNNNNNNGG 5 cut(s) 418, 551, 578, 779, 926
AgsI TTSAA 6 cut(s) 89, 133, 179, 222, 329, 855
AjnI CCWGG 1 cut(s) 778
AjuI GAANNNNNNNTTGG 2 cut(s) 13, 45
AluBI AGCT 7 cut(s) 97, 602, 680, 689, 695, 755, 821
AluI AGCT 7 cut(s) 97, 602, 680, 689, 695, 755, 821
Alw21I GWGCWC 1 cut(s) 586
Alw26I GTCTC 3 cut(s) 48, 54, 432
AlwI GGATC 1 cut(s) 155
AlwNI CAGNNNCTG 1 cut(s) 632
AoxI GGCC 2 cut(s) 91, 452
ApeKI GCWGC 4 cut(s) 548, 677, 755, 821
ApoI RAATTY 1 cut(s) 229
AspS9I GGNCC 1 cut(s) 929
AsuHPI GGTGA 1 cut(s) 711
AvaII GGWCC 1 cut(s) 929
BaeGI GKGCMC 1 cut(s) 811
BalI TGGCCA 1 cut(s) 454
BanI GGYRCC 1 cut(s) 806
BbsI GAAGAC 2 cut(s) 21, 30
Bbv12I GWGCWC 1 cut(s) 586
BbvI GCAGC 4 cut(s) 560, 689, 742, 808
BccI CCATC 6 cut(s) 437, 571, 706, 802, 856, 880
BciT130I CCWGG 1 cut(s) 780
BciVI GTATCC 1 cut(s) 771
BcoDI GTCTC 3 cut(s) 48, 54, 432
BfuAI ACCTGC 1 cut(s) 598
BfuI GTATCC 1 cut(s) 771
BisI GCNGC 6 cut(s) 155, 494, 549, 678, 756, 822
BlsI GCNGC 6 cut(s) 156, 495, 550, 679, 757, 823
Bme1390I CCNGG 1 cut(s) 780
Bme18I GGWCC 1 cut(s) 929
BmgT120I GGNCC 1 cut(s) 929
BmiI GGNNCC 1 cut(s) 808
BmrFI CCNGG 1 cut(s) 780
BmrI ACTGGG 1 cut(s) 19
BmsI GCATC 1 cut(s) 638
BmuI ACTGGG 1 cut(s) 19
BpiI GAAGAC 2 cut(s) 21, 30
BpmI CTGGAG 3 cut(s) 504, 609, 675
Bpu10I CCTNAGC 1 cut(s) 335
BpuEI CTTGAG 2 cut(s) 122, 909
BsaXI ACNNNNNCTCC 4 cut(s) 607, 637, 847, 877
Bsc4I CCNNNNNNNGG 5 cut(s) 418, 551, 578, 779, 926
Bse118I RCCGGY 1 cut(s) 544
Bse1I ACTGG 3 cut(s) 25, 220, 248
BseBI CCWGG 1 cut(s) 780
BseGI GGATG 1 cut(s) 794
BseLI CCNNNNNNNGG 5 cut(s) 418, 551, 578, 779, 926
BseMII CTCAG 1 cut(s) 443
BseNI ACTGG 3 cut(s) 25, 220, 248
BseRI GAGGAG 3 cut(s) 49, 855, 858
BseSI GKGCMC 1 cut(s) 811
BseXI GCAGC 4 cut(s) 560, 689, 742, 808
BseYI CCCAGC 1 cut(s) 685
BsgI GTGCAG 3 cut(s) 552, 576, 819
Bsh1236I CGCG 1 cut(s) 154
BshFI GGCC 2 cut(s) 93, 454
BshNI GGYRCC 1 cut(s) 806
BsiHKAI GWGCWC 1 cut(s) 586
BsiSI CCGG 1 cut(s) 545
BsiWI CGTACG 1 cut(s) 801
BslI CCNNNNNNNGG 5 cut(s) 418, 551, 578, 779, 926
BsmAI GTCTC 3 cut(s) 48, 54, 432
BsmBI CGTCTC 2 cut(s) 48, 54
BsnI GGCC 2 cut(s) 93, 454
Bsp1286I GDGCHC 2 cut(s) 586, 811
Bsp143I GATC 1 cut(s) 160
BspACI CCGC 4 cut(s) 154, 493, 596, 774
BspANI GGCC 2 cut(s) 93, 454
BspCNI CTCAG 1 cut(s) 442
BspFNI CGCG 1 cut(s) 154
BspLI GGNNCC 1 cut(s) 808
BspMI ACCTGC 1 cut(s) 598
BspPI GGATC 1 cut(s) 155
BspT107I GGYRCC 1 cut(s) 806
BsrFI RCCGGY 1 cut(s) 544
BsrI ACTGG 3 cut(s) 25, 220, 248
BssAI RCCGGY 1 cut(s) 544
BssMI GATC 1 cut(s) 160
Bst2UI CCWGG 1 cut(s) 780
Bst4CI ACNGT 3 cut(s) 112, 371, 806
BstAPI GCANNNNNTGC 3 cut(s) 557, 590, 755
BstC8I GCNNGC 3 cut(s) 95, 553, 600
BstDEI CTNAG 2 cut(s) 335, 429
BstENI CCTNNNNNAGG 1 cut(s) 416
BstF5I GGATG 1 cut(s) 794
BstFNI CGCG 1 cut(s) 154
BstKTI GATC 1 cut(s) 163
BstMAI GTCTC 3 cut(s) 48, 54, 432
BstMBI GATC 1 cut(s) 160
BstMWI GCNNNNNNNGC 7 cut(s) 502, 557, 566, 590, 599, 686, 755
BstNI CCWGG 1 cut(s) 780
BstNSI RCATGY 1 cut(s) 73
BstSCI CCNGG 1 cut(s) 778
BstSLI GKGCMC 1 cut(s) 811
BstUI CGCG 1 cut(s) 154
BstV1I GCAGC 4 cut(s) 560, 689, 742, 808
BstV2I GAAGAC 2 cut(s) 21, 30
BstX2I RGATCY 1 cut(s) 160
BstYI RGATCY 1 cut(s) 160
BsuI GTATCC 1 cut(s) 771
BsuRI GGCC 2 cut(s) 93, 454
BtsCI GGATG 1 cut(s) 794
BtsIMutI CAGTG 2 cut(s) 108, 376
BveI ACCTGC 1 cut(s) 598
Cac8I GCNNGC 3 cut(s) 95, 553, 600
CaiI CAGNNNCTG 1 cut(s) 632
Cfr10I RCCGGY 1 cut(s) 544
Cfr13I GGNCC 1 cut(s) 929
Csp6I GTAC 4 cut(s) 407, 447, 610, 802
CviAII CATG 2 cut(s) 70, 257
CviQI GTAC 4 cut(s) 407, 447, 610, 802
DdeI CTNAG 2 cut(s) 335, 429
DpnI GATC 1 cut(s) 162
DpnII GATC 1 cut(s) 160
EaeI YGGCCR 1 cut(s) 452
Eco32I GATATC 1 cut(s) 788
Eco47I GGWCC 1 cut(s) 929
Eco57I CTGAAG 2 cut(s) 398, 443
EcoNI CCTNNNNNAGG 1 cut(s) 416
EcoRII CCWGG 1 cut(s) 778
EcoRV GATATC 1 cut(s) 788
EcoT22I ATGCAT 1 cut(s) 507
Esp3I CGTCTC 2 cut(s) 48, 54
FaeI CATG 2 cut(s) 73, 260
FalI AAGNNNNNCTT 6 cut(s) 81, 113, 396, 428, 480, 512
FatI CATG 2 cut(s) 69, 256
FauI CCCGC 1 cut(s) 781
FauNDI CATATG 3 cut(s) 379, 606, 934
FblI GTMKAC 2 cut(s) 74, 276
Fnu4HI GCNGC 6 cut(s) 155, 494, 549, 678, 756, 822
FokI GGATG 1 cut(s) 781
Fsp4HI GCNGC 6 cut(s) 155, 494, 549, 678, 756, 822
GluI GCNGC 6 cut(s) 155, 494, 549, 678, 756, 822
GsaI CCCAGC 1 cut(s) 689
GsuI CTGGAG 3 cut(s) 504, 609, 675
HaeIII GGCC 2 cut(s) 93, 454
HapII CCGG 1 cut(s) 545
Hin1II CATG 2 cut(s) 73, 260
HincII GTYRAC 1 cut(s) 75
HindII GTYRAC 1 cut(s) 75
HinfI GANTC 2 cut(s) 236, 316
HpaII CCGG 1 cut(s) 545
HphI GGTGA 1 cut(s) 711
Hpy166II GTNNAC 3 cut(s) 75, 277, 409
Hpy188I TCNGA 3 cut(s) 228, 417, 462
Hpy188III TCNNGA 3 cut(s) 101, 313, 358
Hpy8I GTNNAC 3 cut(s) 75, 277, 409
HpyAV CCTTC 5 cut(s) 323, 412, 422, 720, 738
HpyCH4III ACNGT 3 cut(s) 112, 371, 806
HpyCH4IV ACGT 1 cut(s) 168
HpyCH4V TGCA 9 cut(s) 123, 505, 530, 569, 593, 641, 662, 824, 836
HpyF10VI GCNNNNNNNGC 7 cut(s) 502, 557, 566, 590, 599, 686, 755
HpyF3I CTNAG 2 cut(s) 335, 429
HpySE526I ACGT 1 cut(s) 168
Hsp92II CATG 2 cut(s) 73, 260
Kzo9I GATC 1 cut(s) 160
LmnI GCTCC 3 cut(s) 581, 694, 700
Lsp1109I GCAGC 4 cut(s) 560, 689, 742, 808
LweI GCATC 1 cut(s) 638
MaeII ACGT 1 cut(s) 168
MaeIII GTNAC 3 cut(s) 106, 373, 434
MalI GATC 1 cut(s) 162
MboI GATC 1 cut(s) 160
MboII GAAGA 5 cut(s) 21, 24, 30, 795, 843
MflI RGATCY 1 cut(s) 160
MhlI GDGCHC 2 cut(s) 586, 811
MlsI TGGCCA 1 cut(s) 454
MluCI AATT 3 cut(s) 14, 229, 324
MluNI TGGCCA 1 cut(s) 454
MlyI GAGTC 1 cut(s) 230
MmeI TCCRAC 1 cut(s) 44
Mox20I TGGCCA 1 cut(s) 454
Mph1103I ATGCAT 1 cut(s) 507
MscI TGGCCA 1 cut(s) 454
MseI TTAA 1 cut(s) 267
Msp20I TGGCCA 1 cut(s) 454
MspA1I CMGCKG 2 cut(s) 755, 821
MspI CCGG 1 cut(s) 545
MspR9I CCNGG 1 cut(s) 780
MvaI CCWGG 1 cut(s) 780
MvnI CGCG 1 cut(s) 154
MwoI GCNNNNNNNGC 7 cut(s) 502, 557, 566, 590, 599, 686, 755
NdeI CATATG 3 cut(s) 379, 606, 934
NdeII GATC 1 cut(s) 160
NlaIII CATG 2 cut(s) 73, 260
NlaIV GGNNCC 1 cut(s) 808
NmuCI GTSAC 2 cut(s) 106, 373
NsiI ATGCAT 1 cut(s) 507
NspI RCATGY 1 cut(s) 73
PaqCI CACCTGC 1 cut(s) 598
PfeI GAWTC 1 cut(s) 316
Pfl23II CGTACG 1 cut(s) 801
PflMI CCANNNNNTGG 1 cut(s) 578
PkrI GCNGC 6 cut(s) 156, 495, 550, 679, 757, 823
PleI GAGTC 1 cut(s) 230
PpsI GAGTC 1 cut(s) 230
PsiI TTATAA 1 cut(s) 500
Psp6I CCWGG 1 cut(s) 778
PspFI CCCAGC 1 cut(s) 685
PspGI CCWGG 1 cut(s) 778
PspLI CGTACG 1 cut(s) 801
PspN4I GGNNCC 1 cut(s) 808
PspPI GGNCC 1 cut(s) 929
PstNI CAGNNNCTG 1 cut(s) 632
PsuI RGATCY 1 cut(s) 160
PvuII CAGCTG 2 cut(s) 755, 821
RsaI GTAC 4 cut(s) 408, 448, 611, 803
RsaNI GTAC 4 cut(s) 407, 447, 610, 802
SalI GTCGAC 1 cut(s) 73
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 6 cut(s) 155, 494, 549, 678, 756, 822
Sau3AI GATC 1 cut(s) 160
Sau96I GGNCC 1 cut(s) 929
SchI GAGTC 1 cut(s) 230
ScrFI CCNGG 1 cut(s) 780
SduI GDGCHC 2 cut(s) 586, 811
SfaNI GCATC 1 cut(s) 638
SinI GGWCC 1 cut(s) 929
SmlI CTYRAG 2 cut(s) 101, 924
SmoI CTYRAG 2 cut(s) 101, 924
Sse9I AATT 3 cut(s) 14, 229, 324
SsiI CCGC 4 cut(s) 154, 493, 596, 774
StyD4I CCNGG 1 cut(s) 778
TaaI ACNGT 3 cut(s) 112, 371, 806
TaiI ACGT 1 cut(s) 171
TaqI TCGA 3 cut(s) 74, 239, 512
TasI AATT 3 cut(s) 14, 229, 324
TatI WGTACW 2 cut(s) 406, 609
TauI GCSGC 2 cut(s) 157, 496
TfiI GAWTC 1 cut(s) 316
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 2 cut(s) 115, 376
TseFI GTSAC 2 cut(s) 106, 373
TseI GCWGC 4 cut(s) 548, 677, 755, 821
Tsp45I GTSAC 2 cut(s) 106, 373
TspDTI ATGAA 2 cut(s) 359, 720
TspGWI ACGGA 2 cut(s) 36, 294
TspRI CASTG 2 cut(s) 115, 376
Van91I CCANNNNNTGG 1 cut(s) 578
VpaK11BI GGWCC 1 cut(s) 929
XagI CCTNNNNNAGG 1 cut(s) 416
XapI RAATTY 1 cut(s) 229
XceI RCATGY 1 cut(s) 73
XmiI GTMKAC 2 cut(s) 74, 276
Zsp2I ATGCAT 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.