FvH4_2g08100

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010523
Physical Location & Seq
Forward (+)
7006719 .. 7009246
2528 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g08100.t1

Sequence Viewer

Length: 1257 bp
ATGGGCGGTGTTCAAGCAAAGGCTGCAAAGCCCGATAGACGACGACGCCCCGTTTTGGAGCGGAAGACATCCTTTGCTAAGAAGGTTGTTCCCATCATCACTTCCGAACCAAGTGATTCAAGTAGTGAAGACTTGAATCACTTGATGAGATCGTATCAGGCGGATTGCGAAGATGACAAAGACTTGCGAGACTTCGACGACAAACTCAGAACCGGCGCCGAAGAAGTAGCCAGGCACAGAACCACCGGTTTTGATCGGTTGAATAAATGCCTCAAGTATCTGACGCAGTCCAACGAGAAGATTGTGAGCGAAATCTCGGCATGCACACAAAAGATACTTGAGGCAAAGGGTGAGGAGGCGGGTATGGTGATGACGGCAAATGAGAAGCTCTTGGATCATTTGATGACAGAGTACGTGGGCAATAGCTGGCAGACGCTTCAACTCTGCGAAAAGGAATTGCAAGACTTTATCAAGTCGGCTCATGATGTCCATTCATGTATCGCAGATGTGGTACGTGATGGGGAAGTGTCCAAGAAGCTCAACGAGTCTATAATGAAATATCACCATTCTGCAAAAGCAGTTGTTGCAAAGGTGAACTATTTGCAGGACCAACATGCAGACATGCTTAACAAGTTGAAAGAACAAATTCTCAAGTTTGATAAGAAGGTGGAGCTTGCTCGTACTTTAAAGAAGGTTGTAAATGTGGTGTTCATAGGTATGTTGTCTGCTCTCTTTGTTTGCACCATCATGGCGGCAGCTATGGCTGCTCCGCCAGTTGCTTCGGCCTTGACCACTCATCCATGTATAGCCGGAGTTGTTGCTGGTGGTGCCGTTGTTTATGCTTGTTCGCCAGTATTCAAAGAGGGACGACACTGGCTTCTTAATCTGATTCAAGATTATGAGTCAACTCTGGAGGCACAGAAGGGTCTAATTAAGTCGATGGAAGCCGGAACCGGAGAAGCCATTAAAGAGTTGGATGGCATCATGTCTCTTGTTGAGAAGGTCATTGCAAAGGGGAATGATCAAGTATTGTCCAAATTAAACTCTGCGAATGATGAGGGAAGAGTAGATTTGAGAATTGAGGATCCGGAAGTAATTATCATGGGAAAGCTGGCAGACTTTGACCAAGAAATTAAGGAATTGGACAAGAGAAGAGAGGAGTGCCTCAAGATTACAGGAAAGGCAAGGGATCAGGTTTTGATGGAATATGGCGAGTTGAAGAGAAGTGAAGTGTGGAACACGAAGACCAAGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

419

Amino Acids

46.73

Weight (kDa)

6.41

Isoelectric Point (pI)

42.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 79 - 400 2.9e-22 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 215, 827
AccBSI CCGCTC 1 cut(s) 61
AccIII TCCGGA 1 cut(s) 1087
AciI CCGC 6 cut(s) 6, 61, 161, 359, 752, 770
AclWI GGATC 4 cut(s) 402, 1079, 1092, 1197
AcsI RAATTY 1 cut(s) 645
AcyI GRCGYC 2 cut(s) 46, 216
AfaI GTAC 3 cut(s) 413, 513, 682
AfiI CCNNNNNNNGG 1 cut(s) 55
AgeI ACCGGT 1 cut(s) 245
AgsI TTSAA 9 cut(s) 14, 120, 136, 262, 440, 637, 859, 893, 1219
AjnI CCWGG 1 cut(s) 230
AluBI AGCT 6 cut(s) 388, 426, 538, 673, 758, 1111
AluI AGCT 6 cut(s) 388, 426, 538, 673, 758, 1111
Alw26I GTCTC 2 cut(s) 183, 993
AlwI GGATC 4 cut(s) 402, 1079, 1092, 1197
Aor13HI TCCGGA 1 cut(s) 1087
AoxI GGCC 1 cut(s) 783
ApeKI GCWGC 3 cut(s) 23, 755, 764
ApoI RAATTY 1 cut(s) 645
AsiGI ACCGGT 1 cut(s) 245
Asp700I GAANNNNTTC 1 cut(s) 645
AspLEI GCGC 1 cut(s) 218
AspS9I GGNCC 1 cut(s) 607
AsuHPI GGTGA 4 cut(s) 362, 379, 554, 604
AvaII GGWCC 1 cut(s) 607
BamHI GGATCC 1 cut(s) 1084
BanI GGYRCC 2 cut(s) 215, 827
BbsI GAAGAC 3 cut(s) 71, 135, 1250
BbvI GCAGC 3 cut(s) 10, 751, 767
BccI CCATC 6 cut(s) 101, 512, 752, 934, 971, 1195
BceAI ACGGC 2 cut(s) 390, 815
BciT130I CCWGG 1 cut(s) 232
BclI TGATCA 1 cut(s) 1021
BcoDI GTCTC 2 cut(s) 183, 993
BfoI RGCGCY 1 cut(s) 219
BisI GCNGC 4 cut(s) 24, 753, 756, 765
BlsI GCNGC 4 cut(s) 25, 754, 757, 766
Bme1390I CCNGG 1 cut(s) 232
Bme18I GGWCC 1 cut(s) 607
BmgT120I GGNCC 1 cut(s) 607
BmiI GGNNCC 4 cut(s) 217, 829, 952, 1086
BmrFI CCNGG 1 cut(s) 232
BmsI GCATC 1 cut(s) 990
BpiI GAAGAC 3 cut(s) 71, 135, 1250
BpmI CTGGAG 1 cut(s) 932
BpuEI CTTGAG 4 cut(s) 257, 359, 635, 1151
BsaAI YACGTR 2 cut(s) 415, 515
BsaHI GRCGYC 2 cut(s) 46, 216
BsaWI WCCGGW 3 cut(s) 245, 953, 1087
Bsc4I CCNNNNNNNGG 1 cut(s) 55
Bse118I RCCGGY 2 cut(s) 212, 245
Bse1I ACTGG 3 cut(s) 773, 851, 878
Bse3DI GCAATG 1 cut(s) 1005
BseAI TCCGGA 1 cut(s) 1087
BseBI CCWGG 1 cut(s) 232
BseGI GGATG 3 cut(s) 68, 796, 982
BseLI CCNNNNNNNGG 1 cut(s) 55
BseMI GCAATG 1 cut(s) 1005
BseMII CTCAG 1 cut(s) 220
BseNI ACTGG 3 cut(s) 773, 851, 878
BseRI GAGGAG 2 cut(s) 368, 1172
BseXI GCAGC 3 cut(s) 10, 751, 767
BshFI GGCC 1 cut(s) 785
BshNI GGYRCC 2 cut(s) 215, 827
BshTI ACCGGT 1 cut(s) 245
BsiSI CCGG 6 cut(s) 213, 246, 810, 948, 954, 1088
BslFI GGGAC 1 cut(s) 879
BslI CCNNNNNNNGG 1 cut(s) 55
BsmAI GTCTC 2 cut(s) 183, 993
BsmFI GGGAC 1 cut(s) 879
BsnI GGCC 1 cut(s) 785
Bsp13I TCCGGA 1 cut(s) 1087
Bsp143I GATC 6 cut(s) 149, 253, 394, 1021, 1084, 1189
BspACI CCGC 6 cut(s) 6, 61, 161, 359, 752, 770
BspANI GGCC 1 cut(s) 785
BspCNI CTCAG 1 cut(s) 219
BspEI TCCGGA 1 cut(s) 1087
BspHI TCATGA 1 cut(s) 481
BspLI GGNNCC 4 cut(s) 217, 829, 952, 1086
BspPI GGATC 4 cut(s) 402, 1079, 1092, 1197
BspT107I GGYRCC 2 cut(s) 215, 827
BsrBI CCGCTC 1 cut(s) 61
BsrDI GCAATG 1 cut(s) 1005
BsrFI RCCGGY 2 cut(s) 212, 245
BsrI ACTGG 3 cut(s) 773, 851, 878
BssAI RCCGGY 2 cut(s) 212, 245
BssMI GATC 6 cut(s) 149, 253, 394, 1021, 1084, 1189
BssNI GRCGYC 2 cut(s) 46, 216
Bst2UI CCWGG 1 cut(s) 232
Bst6I CTCTTC 3 cut(s) 1057, 1147, 1214
BstACI GRCGYC 2 cut(s) 46, 216
BstAPI GCANNNNNTGC 2 cut(s) 23, 584
BstBAI YACGTR 2 cut(s) 415, 515
BstC8I GCNNGC 4 cut(s) 322, 428, 675, 1113
BstDEI CTNAG 2 cut(s) 78, 206
BstF5I GGATG 3 cut(s) 68, 796, 982
BstH2I RGCGCY 1 cut(s) 219
BstHHI GCGC 1 cut(s) 218
BstKTI GATC 6 cut(s) 152, 256, 397, 1024, 1087, 1192
BstMAI GTCTC 2 cut(s) 183, 993
BstMBI GATC 6 cut(s) 149, 253, 394, 1021, 1084, 1189
BstMWI GCNNNNNNNGC 5 cut(s) 23, 584, 761, 764, 827
BstNI CCWGG 1 cut(s) 232
BstNSI RCATGY 3 cut(s) 324, 617, 625
BstSCI CCNGG 1 cut(s) 230
BstV1I GCAGC 3 cut(s) 10, 751, 767
BstV2I GAAGAC 3 cut(s) 71, 135, 1250
BstX2I RGATCY 1 cut(s) 1084
BstYI RGATCY 1 cut(s) 1084
BsuRI GGCC 1 cut(s) 785
BtsCI GGATG 3 cut(s) 68, 796, 982
BtsIMutI CAGTG 1 cut(s) 871
Cac8I GCNNGC 4 cut(s) 322, 428, 675, 1113
CciI TCATGA 1 cut(s) 481
CfoI GCGC 1 cut(s) 218
Cfr10I RCCGGY 2 cut(s) 212, 245
Cfr13I GGNCC 1 cut(s) 607
CseI GACGC 3 cut(s) 54, 292, 442
Csp6I GTAC 3 cut(s) 412, 512, 681
CspAI ACCGGT 1 cut(s) 245
CspCI CAANNNNNGTGG 2 cut(s) 232, 267
CviAII CATG 9 cut(s) 321, 482, 495, 614, 622, 748, 801, 985, 1102
CviQI GTAC 3 cut(s) 412, 512, 681
DdeI CTNAG 2 cut(s) 78, 206
DinI GGCGCC 1 cut(s) 217
DpnI GATC 6 cut(s) 151, 255, 396, 1023, 1086, 1191
DpnII GATC 6 cut(s) 149, 253, 394, 1021, 1084, 1189
DraI TTTAAA 1 cut(s) 687
Eam1104I CTCTTC 3 cut(s) 1057, 1147, 1214
EarI CTCTTC 3 cut(s) 1057, 1147, 1214
EciI GGCGGA 2 cut(s) 176, 759
Eco47I GGWCC 1 cut(s) 607
EcoRII CCWGG 1 cut(s) 230
EgeI GGCGCC 1 cut(s) 217
EheI GGCGCC 1 cut(s) 217
FaeI CATG 9 cut(s) 324, 485, 498, 617, 625, 751, 804, 988, 1105
FalI AAGNNNNNCTT 2 cut(s) 56, 88
FaqI GGGAC 1 cut(s) 879
FatI CATG 9 cut(s) 320, 481, 494, 613, 621, 747, 800, 984, 1101
FauI CCCGC 1 cut(s) 352
FbaI TGATCA 1 cut(s) 1021
Fnu4HI GCNGC 4 cut(s) 24, 753, 756, 765
FokI GGATG 3 cut(s) 55, 783, 989
Fsp4HI GCNGC 4 cut(s) 24, 753, 756, 765
GlaI GCGC 1 cut(s) 217
GluI GCNGC 4 cut(s) 24, 753, 756, 765
GsuI CTGGAG 1 cut(s) 932
HaeII RGCGCY 1 cut(s) 219
HaeIII GGCC 1 cut(s) 785
HapII CCGG 6 cut(s) 213, 246, 810, 948, 954, 1088
HgaI GACGC 3 cut(s) 54, 292, 442
HhaI GCGC 1 cut(s) 218
Hin1I GRCGYC 2 cut(s) 46, 216
Hin1II CATG 9 cut(s) 324, 485, 498, 617, 625, 751, 804, 988, 1105
Hin6I GCGC 1 cut(s) 216
HinP1I GCGC 1 cut(s) 216
HincII GTYRAC 1 cut(s) 906
HindII GTYRAC 1 cut(s) 906
HinfI GANTC 5 cut(s) 116, 136, 545, 889, 902
HpaII CCGG 6 cut(s) 213, 246, 810, 948, 954, 1088
HphI GGTGA 4 cut(s) 362, 379, 554, 604
Hpy166II GTNNAC 2 cut(s) 595, 906
Hpy188I TCNGA 4 cut(s) 106, 209, 282, 888
Hpy188III TCNNGA 5 cut(s) 482, 893, 911, 1088, 1168
Hpy8I GTNNAC 2 cut(s) 595, 906
Hpy99I CGWCG 3 cut(s) 45, 48, 200
HpyAV CCTTC 5 cut(s) 76, 658, 685, 916, 994
HpyCH4IV ACGT 2 cut(s) 414, 514
HpyCH4V TGCA 9 cut(s) 26, 324, 460, 572, 587, 604, 617, 741, 1010
HpyF10VI GCNNNNNNNGC 5 cut(s) 23, 584, 761, 764, 827
HpyF3I CTNAG 2 cut(s) 78, 206
HpySE526I ACGT 2 cut(s) 414, 514
Hsp92I GRCGYC 2 cut(s) 46, 216
Hsp92II CATG 9 cut(s) 324, 485, 498, 617, 625, 751, 804, 988, 1105
HspAI GCGC 1 cut(s) 216
KasI GGCGCC 1 cut(s) 215
Kpn2I TCCGGA 1 cut(s) 1087
Ksp22I TGATCA 1 cut(s) 1021
Kzo9I GATC 6 cut(s) 149, 253, 394, 1021, 1084, 1189
LmnI GCTCC 3 cut(s) 58, 670, 772
Lsp1109I GCAGC 3 cut(s) 10, 751, 767
LweI GCATC 1 cut(s) 990
MaeII ACGT 2 cut(s) 414, 514
MalI GATC 6 cut(s) 151, 255, 396, 1023, 1086, 1191
MbiI CCGCTC 1 cut(s) 61
MboI GATC 6 cut(s) 149, 253, 394, 1021, 1084, 1189
MboII GAAGA 9 cut(s) 76, 140, 182, 233, 310, 1074, 1164, 1231, 1255
MflI RGATCY 1 cut(s) 1084
MluCI AATT 8 cut(s) 455, 645, 930, 1037, 1077, 1095, 1131, 1139
Mly113I GGCGCC 1 cut(s) 216
MlyI GAGTC 2 cut(s) 554, 911
MmeI TCCRAC 2 cut(s) 315, 954
MroI TCCGGA 1 cut(s) 1087
MroXI GAANNNNTTC 1 cut(s) 645
MseI TTAA 7 cut(s) 627, 686, 882, 933, 966, 1040, 1134
MslI CAYNNNNRTG 2 cut(s) 716, 746
MspI CCGG 6 cut(s) 213, 246, 810, 948, 954, 1088
MspR9I CCNGG 1 cut(s) 232
MvaI CCWGG 1 cut(s) 232
MwoI GCNNNNNNNGC 5 cut(s) 23, 584, 761, 764, 827
NarI GGCGCC 1 cut(s) 216
NdeII GATC 6 cut(s) 149, 253, 394, 1021, 1084, 1189
NlaIII CATG 9 cut(s) 324, 485, 498, 617, 625, 751, 804, 988, 1105
NlaIV GGNNCC 4 cut(s) 217, 829, 952, 1086
NmeAIII GCCGAG 1 cut(s) 296
NspI RCATGY 3 cut(s) 324, 617, 625
PaeI GCATGC 1 cut(s) 324
PagI TCATGA 1 cut(s) 481
PdmI GAANNNNTTC 1 cut(s) 645
PfeI GAWTC 3 cut(s) 116, 136, 889
PflFI GACNNNGTC 1 cut(s) 286
PinAI ACCGGT 1 cut(s) 245
PkrI GCNGC 4 cut(s) 25, 754, 757, 766
PleI GAGTC 2 cut(s) 553, 910
PluTI GGCGCC 1 cut(s) 219
PpsI GAGTC 2 cut(s) 553, 910
Ppu21I YACGTR 2 cut(s) 415, 515
Psp6I CCWGG 1 cut(s) 230
PspGI CCWGG 1 cut(s) 230
PspN4I GGNNCC 4 cut(s) 217, 829, 952, 1086
PspPI GGNCC 1 cut(s) 607
PsuI RGATCY 1 cut(s) 1084
PsyI GACNNNGTC 1 cut(s) 286
RsaI GTAC 3 cut(s) 413, 513, 682
RsaNI GTAC 3 cut(s) 412, 512, 681
RseI CAYNNNNRTG 2 cut(s) 716, 746
SaqAI TTAA 7 cut(s) 627, 686, 882, 933, 966, 1040, 1134
SatI GCNGC 4 cut(s) 24, 753, 756, 765
Sau3AI GATC 6 cut(s) 149, 253, 394, 1021, 1084, 1189
Sau96I GGNCC 1 cut(s) 607
SchI GAGTC 2 cut(s) 554, 911
ScrFI CCNGG 1 cut(s) 232
SfaNI GCATC 1 cut(s) 990
SfoI GGCGCC 1 cut(s) 217
SinI GGWCC 1 cut(s) 607
SmiMI CAYNNNNRTG 2 cut(s) 716, 746
SmlI CTYRAG 4 cut(s) 272, 338, 650, 1166
SmoI CTYRAG 4 cut(s) 272, 338, 650, 1166
SphI GCATGC 1 cut(s) 324
Sse9I AATT 8 cut(s) 455, 645, 930, 1037, 1077, 1095, 1131, 1139
SsiI CCGC 6 cut(s) 6, 61, 161, 359, 752, 770
SspDI GGCGCC 1 cut(s) 215
StyD4I CCNGG 1 cut(s) 230
TaiI ACGT 2 cut(s) 417, 517
TaqI TCGA 2 cut(s) 195, 938
TasI AATT 8 cut(s) 455, 645, 930, 1037, 1077, 1095, 1131, 1139
TauI GCSGC 1 cut(s) 755
TfiI GAWTC 3 cut(s) 116, 136, 889
Tru1I TTAA 7 cut(s) 627, 686, 882, 933, 966, 1040, 1134
Tru9I TTAA 7 cut(s) 627, 686, 882, 933, 966, 1040, 1134
TscAI CASTG 1 cut(s) 878
TseI GCWGC 3 cut(s) 23, 755, 764
TspDTI ATGAA 3 cut(s) 483, 569, 700
TspRI CASTG 1 cut(s) 878
Tth111I GACNNNGTC 1 cut(s) 286
VpaK11BI GGWCC 1 cut(s) 607
XapI RAATTY 1 cut(s) 645
XceI RCATGY 3 cut(s) 324, 617, 625
XcmI CCANNNNNNNNNTGG 1 cut(s) 970
XmnI GAANNNNTTC 1 cut(s) 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.