RchiOBHm_Chr6g0262911

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0010523
Physical Location & Seq
Forward (+)
17689315 .. 17690628
1314 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23579

Sequence Viewer

Length: 1224 bp
ATGGGCCGTGTTCAAGCAAAGCCAGAGAGCGGACGTGCCGGTTTGGAGCGAAAGACATCCCTTGCTAAGGTTGTTCCCATCAATGCCGAATCAAGGCCAACTGGCGACCTCTGCAAGGATTTTGAAGACTTGATGAGATCGTATCAGGCGGATTGCAAAGATGACAAAGACTTGCAAGACTTCGACGCCGAACTCAGCTCCAGCGCCAAAGAAGTCTTCTGCAGTACCGGTTTTGATCGGCTTGAGAAATACCTAAAGTATCTGAAGGATTCCGACGAGAAGATGAAGGAGGTAATCTTGTCATCCATAACACAGATACGTGAGGCAGAGGATGTGCAGGCGATGACGGAGAATGAGAAGATCTTAGATGATTTGATCAAAATGTACACCAACAATAGCGGGCAGACGCATCAACTCTGCGGAGCGGAATTGCGAATTTTCATCGACTCGGCTCGTGCGGTCCATTCATGCATTACAAATGTGCTTGAGAGGGGAGTGTCGAAGAAGTTCAACGAGTCTGTGAGGAAGTATCGCCTTGATGCTGAAGCAGTAGCTGCAAAAGTGAAATCCCTGCGCGACCAACACACAGACATGCTTCACCAGTTGAAAGATCAAATTCAGAAGTGTGATAAGAAGCTGGAGCGTACTCGTACTTTAAAGAAGGTCGTAAATGTGGTGTTCATAGGTATGTTGTCTGGTCTGTTTGTTTGCACCATCATGGCGGCGGCTATGGCTGCTCCGCCAGTTGCGTCAGCCTTGACCACTCATCCATGTATTGCCGGAGTTATTGCTGGTGGTGCCGTTGGTTATGCTTGTTCGTCAGTATTAAAAGAGGGACGACACTGGCTTCTCACTTTGATTCAAGATTGTGAATCAACTCTGGAGGCACATAAGGGTGTAATTCAGTCGATGGAAGCCGGAACCGGAGTCGCCATTAAAGAGTTGGATGACATCATTTGTCTTGTTGAGAAGGTCATTGCAAAGGGGAATGATCCAGTGTTGTCCAATTTATGCTCTGCAAATGATAAGGGAAGAGTAGATGTGGAAATTGATGAGAAAGAGATTATTGAAAAAAAGCGGACAGAGTTTCACCAAGCAATTGAGGAATTGGACAAGAAAAGAGAGGACTGCCTCAGGGTTGTAGAGAAGGCAAGCAAAAAGGTTCAAAGGGAATATGACGAATTGAAGAGGAGCCCAGTGTTAAAGACGAAGATCAAGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

407

Amino Acids

45.44

Weight (kDa)

7.06

Isoelectric Point (pI)

35.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 73 - 388 6.3e-23 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 797
AccBSI CCGCTC 2 cut(s) 30, 425
AccII CGCG 1 cut(s) 576
AclWI GGATC 1 cut(s) 986
AcsI RAATTY 2 cut(s) 435, 615
AcuI CTGAAG 2 cut(s) 284, 564
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 4 cut(s) 226, 386, 646, 652
AfiI CCNNNNNNNGG 4 cut(s) 29, 67, 93, 115
AgeI ACCGGT 1 cut(s) 227
AgsI TTSAA 8 cut(s) 14, 125, 511, 607, 863, 1070, 1166, 1186
AjiI CACGTC 1 cut(s) 35
AjuI GAANNNNNNNTTGG 2 cut(s) 200, 232
AluBI AGCT 3 cut(s) 198, 554, 637
AluI AGCT 3 cut(s) 198, 554, 637
AlwI GGATC 1 cut(s) 986
AlwNI CAGNNNCTG 1 cut(s) 554
AoxI GGCC 2 cut(s) 4, 95
ApeKI GCWGC 2 cut(s) 554, 734
ApoI RAATTY 2 cut(s) 435, 615
AsiGI ACCGGT 1 cut(s) 227
Asp700I GAANNNNTTC 1 cut(s) 506
AspLEI GCGC 2 cut(s) 206, 576
AspS9I GGNCC 2 cut(s) 4, 460
AsuHPI GGTGA 2 cut(s) 590, 1082
AvaII GGWCC 1 cut(s) 460
AxyI CCTNAGG 1 cut(s) 1133
BanI GGYRCC 1 cut(s) 797
BanII GRGCYC 1 cut(s) 1196
BauI CACGAG 1 cut(s) 453
BbsI GAAGAC 2 cut(s) 132, 208
BbvI GCAGC 2 cut(s) 541, 721
BccI CCATC 3 cut(s) 86, 722, 904
BceAI ACGGC 1 cut(s) 785
BclI TGATCA 1 cut(s) 375
BfmI CTRYAG 1 cut(s) 220
BfoI RGCGCY 1 cut(s) 207
BglII AGATCT 1 cut(s) 360
BisI GCNGC 4 cut(s) 555, 723, 726, 735
BlsI GCNGC 4 cut(s) 556, 724, 727, 736
Bme18I GGWCC 1 cut(s) 460
BmgBI CACGTC 1 cut(s) 35
BmgT120I GGNCC 2 cut(s) 4, 460
BmiI GGNNCC 3 cut(s) 799, 922, 1193
BmrI ACTGGG 1 cut(s) 1190
BmsI GCATC 2 cut(s) 418, 529
BmuI ACTGGG 1 cut(s) 1190
BpiI GAAGAC 2 cut(s) 132, 208
BpmI CTGGAG 3 cut(s) 184, 659, 902
Bpu10I CCTNAGC 1 cut(s) 66
BpuEI CTTGAG 2 cut(s) 263, 506
BsaAI YACGTR 1 cut(s) 320
BsaHI GRCGYC 1 cut(s) 186
BsaWI WCCGGW 2 cut(s) 227, 923
Bsc4I CCNNNNNNNGG 4 cut(s) 29, 67, 93, 115
Bse118I RCCGGY 2 cut(s) 38, 227
Bse1I ACTGG 6 cut(s) 106, 601, 743, 848, 995, 1196
Bse21I CCTNAGG 1 cut(s) 1133
Bse3DI GCAATG 1 cut(s) 975
BseGI GGATG 5 cut(s) 56, 302, 337, 766, 952
BseLI CCNNNNNNNGG 4 cut(s) 29, 67, 93, 115
BseMI GCAATG 1 cut(s) 975
BseMII CTCAG 2 cut(s) 208, 1147
BseNI ACTGG 6 cut(s) 106, 601, 743, 848, 995, 1196
BseRI GAGGAG 1 cut(s) 1204
BseXI GCAGC 2 cut(s) 541, 721
BsgI GTGCAG 1 cut(s) 356
Bsh1236I CGCG 1 cut(s) 576
BshFI GGCC 2 cut(s) 6, 97
BshNI GGYRCC 1 cut(s) 797
BshTI ACCGGT 1 cut(s) 227
BsiSI CCGG 5 cut(s) 39, 228, 780, 918, 924
BslFI GGGAC 1 cut(s) 849
BslI CCNNNNNNNGG 4 cut(s) 29, 67, 93, 115
BsmFI GGGAC 1 cut(s) 849
BsnI GGCC 2 cut(s) 6, 97
Bsp1286I GDGCHC 1 cut(s) 1196
Bsp1407I TGTACA 1 cut(s) 384
Bsp143I GATC 7 cut(s) 137, 235, 360, 375, 610, 991, 1212
BspANI GGCC 2 cut(s) 6, 97
BspCNI CTCAG 2 cut(s) 207, 1146
BspFNI CGCG 1 cut(s) 576
BspLI GGNNCC 3 cut(s) 799, 922, 1193
BspMAI CTGCAG 1 cut(s) 224
BspPI GGATC 1 cut(s) 986
BspT107I GGYRCC 1 cut(s) 797
BsrBI CCGCTC 2 cut(s) 30, 425
BsrDI GCAATG 1 cut(s) 975
BsrFI RCCGGY 2 cut(s) 38, 227
BsrGI TGTACA 1 cut(s) 384
BsrI ACTGG 6 cut(s) 106, 601, 743, 848, 995, 1196
BssAI RCCGGY 2 cut(s) 38, 227
BssMI GATC 7 cut(s) 137, 235, 360, 375, 610, 991, 1212
BssNI GRCGYC 1 cut(s) 186
BssSI CACGAG 1 cut(s) 453
Bst2BI CACGAG 1 cut(s) 453
Bst6I CTCTTC 2 cut(s) 1027, 1181
BstACI GRCGYC 1 cut(s) 186
BstAPI GCANNNNNTGC 1 cut(s) 554
BstAUI TGTACA 1 cut(s) 384
BstBAI YACGTR 1 cut(s) 320
BstC8I GCNNGC 3 cut(s) 339, 401, 1153
BstDEI CTNAG 4 cut(s) 66, 194, 364, 1133
BstENI CCTNNNNNAGG 2 cut(s) 65, 113
BstF5I GGATG 5 cut(s) 56, 302, 337, 766, 952
BstFNI CGCG 1 cut(s) 576
BstH2I RGCGCY 1 cut(s) 207
BstHHI GCGC 2 cut(s) 206, 576
BstKTI GATC 7 cut(s) 140, 238, 363, 378, 613, 994, 1215
BstMBI GATC 7 cut(s) 137, 235, 360, 375, 610, 991, 1212
BstMWI GCNNNNNNNGC 5 cut(s) 111, 554, 731, 734, 797
BstNSI RCATGY 1 cut(s) 595
BstSFI CTRYAG 1 cut(s) 220
BstUI CGCG 1 cut(s) 576
BstV1I GCAGC 2 cut(s) 541, 721
BstV2I GAAGAC 2 cut(s) 132, 208
BstX2I RGATCY 1 cut(s) 360
BstYI RGATCY 1 cut(s) 360
Bsu36I CCTNAGG 1 cut(s) 1133
BsuRI GGCC 2 cut(s) 6, 97
BtgZI GCGATG 1 cut(s) 356
BtrI CACGTC 1 cut(s) 35
BtsCI GGATG 5 cut(s) 56, 302, 337, 766, 952
BtsIMutI CAGTG 3 cut(s) 841, 1002, 1203
Cac8I GCNNGC 3 cut(s) 339, 401, 1153
CaiI CAGNNNCTG 1 cut(s) 554
CfoI GCGC 2 cut(s) 206, 576
Cfr10I RCCGGY 2 cut(s) 38, 227
Cfr13I GGNCC 2 cut(s) 4, 460
CseI GACGC 3 cut(s) 194, 415, 738
Csp6I GTAC 4 cut(s) 225, 385, 645, 651
CspAI ACCGGT 1 cut(s) 227
CviAII CATG 4 cut(s) 468, 592, 718, 771
CviQI GTAC 4 cut(s) 225, 385, 645, 651
DdeI CTNAG 4 cut(s) 66, 194, 364, 1133
DpnI GATC 7 cut(s) 139, 237, 362, 377, 612, 993, 1214
DpnII GATC 7 cut(s) 137, 235, 360, 375, 610, 991, 1212
DraI TTTAAA 1 cut(s) 657
Eam1104I CTCTTC 2 cut(s) 1027, 1181
EarI CTCTTC 2 cut(s) 1027, 1181
EciI GGCGGA 2 cut(s) 164, 729
Eco24I GRGCYC 1 cut(s) 1196
Eco47I GGWCC 1 cut(s) 460
Eco57I CTGAAG 2 cut(s) 284, 564
Eco81I CCTNAGG 1 cut(s) 1133
EcoNI CCTNNNNNAGG 2 cut(s) 65, 113
EcoT22I ATGCAT 1 cut(s) 473
EcoT38I GRGCYC 1 cut(s) 1196
FaeI CATG 4 cut(s) 471, 595, 721, 774
FaqI GGGAC 1 cut(s) 849
FatI CATG 4 cut(s) 467, 591, 717, 770
FauI CCCGC 1 cut(s) 392
FbaI TGATCA 1 cut(s) 375
Fnu4HI GCNGC 4 cut(s) 555, 723, 726, 735
FokI GGATG 5 cut(s) 43, 289, 344, 753, 959
FriOI GRGCYC 1 cut(s) 1196
Fsp4HI GCNGC 4 cut(s) 555, 723, 726, 735
GlaI GCGC 2 cut(s) 205, 575
GluI GCNGC 4 cut(s) 555, 723, 726, 735
GsuI CTGGAG 3 cut(s) 184, 659, 902
HaeII RGCGCY 1 cut(s) 207
HaeIII GGCC 2 cut(s) 6, 97
HapII CCGG 5 cut(s) 39, 228, 780, 918, 924
HgaI GACGC 3 cut(s) 194, 415, 738
HhaI GCGC 2 cut(s) 206, 576
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 4 cut(s) 471, 595, 721, 774
Hin6I GCGC 2 cut(s) 204, 574
HinP1I GCGC 2 cut(s) 204, 574
HinfI GANTC 7 cut(s) 89, 269, 446, 515, 859, 872, 927
HpaII CCGG 5 cut(s) 39, 228, 780, 918, 924
HphI GGTGA 2 cut(s) 590, 1082
Hpy166II GTNNAC 1 cut(s) 387
Hpy188I TCNGA 3 cut(s) 264, 274, 621
Hpy188III TCNNGA 3 cut(s) 863, 881, 1216
Hpy8I GTNNAC 1 cut(s) 387
Hpy99I CGWCG 2 cut(s) 188, 278
HpyAV CCTTC 5 cut(s) 259, 280, 655, 964, 1141
HpyCH4IV ACGT 2 cut(s) 34, 319
HpyF10VI GCNNNNNNNGC 5 cut(s) 111, 554, 731, 734, 797
HpyF3I CTNAG 4 cut(s) 66, 194, 364, 1133
HpySE526I ACGT 2 cut(s) 34, 319
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 4 cut(s) 471, 595, 721, 774
HspAI GCGC 2 cut(s) 204, 574
Ksp22I TGATCA 1 cut(s) 375
Kzo9I GATC 7 cut(s) 137, 235, 360, 375, 610, 991, 1212
LmnI GCTCC 6 cut(s) 46, 203, 422, 640, 742, 1191
Lsp1109I GCAGC 2 cut(s) 541, 721
LweI GCATC 2 cut(s) 418, 529
MaeII ACGT 2 cut(s) 34, 319
MalI GATC 7 cut(s) 139, 237, 362, 377, 612, 993, 1214
MbiI CCGCTC 2 cut(s) 30, 425
MboI GATC 7 cut(s) 137, 235, 360, 375, 610, 991, 1212
MboII GAAGA 8 cut(s) 137, 208, 292, 370, 514, 1044, 1198, 1222
MfeI CAATTG 1 cut(s) 1098
MflI RGATCY 1 cut(s) 360
MhlI GDGCHC 1 cut(s) 1196
MluCI AATT 9 cut(s) 428, 435, 615, 900, 1006, 1047, 1098, 1106, 1181
MlyI GAGTC 3 cut(s) 440, 524, 936
MmeI TCCRAC 2 cut(s) 297, 924
Mph1103I ATGCAT 1 cut(s) 473
MroXI GAANNNNTTC 1 cut(s) 506
MseI TTAA 4 cut(s) 656, 827, 936, 1202
MslI CAYNNNNRTG 4 cut(s) 590, 686, 716, 894
MspI CCGG 5 cut(s) 39, 228, 780, 918, 924
MunI CAATTG 1 cut(s) 1098
MvnI CGCG 1 cut(s) 576
MwoI GCNNNNNNNGC 5 cut(s) 111, 554, 731, 734, 797
NdeII GATC 7 cut(s) 137, 235, 360, 375, 610, 991, 1212
NlaIII CATG 4 cut(s) 471, 595, 721, 774
NlaIV GGNNCC 3 cut(s) 799, 922, 1193
NmeAIII GCCGAG 1 cut(s) 428
NsiI ATGCAT 1 cut(s) 473
NspI RCATGY 1 cut(s) 595
PdmI GAANNNNTTC 1 cut(s) 506
PfeI GAWTC 4 cut(s) 89, 269, 859, 872
PinAI ACCGGT 1 cut(s) 227
PkrI GCNGC 4 cut(s) 556, 724, 727, 736
PleI GAGTC 3 cut(s) 440, 523, 935
PpsI GAGTC 3 cut(s) 440, 523, 935
Ppu21I YACGTR 1 cut(s) 320
PspN4I GGNNCC 3 cut(s) 799, 922, 1193
PspPI GGNCC 2 cut(s) 4, 460
PstI CTGCAG 1 cut(s) 224
PstNI CAGNNNCTG 1 cut(s) 554
PsuI RGATCY 1 cut(s) 360
RsaI GTAC 4 cut(s) 226, 386, 646, 652
RsaNI GTAC 4 cut(s) 225, 385, 645, 651
RseI CAYNNNNRTG 4 cut(s) 590, 686, 716, 894
SaqAI TTAA 4 cut(s) 656, 827, 936, 1202
SatI GCNGC 4 cut(s) 555, 723, 726, 735
Sau3AI GATC 7 cut(s) 137, 235, 360, 375, 610, 991, 1212
Sau96I GGNCC 2 cut(s) 4, 460
SchI GAGTC 3 cut(s) 440, 524, 936
SduI GDGCHC 1 cut(s) 1196
SfaNI GCATC 2 cut(s) 418, 529
SfcI CTRYAG 1 cut(s) 220
SinI GGWCC 1 cut(s) 460
SmiMI CAYNNNNRTG 4 cut(s) 590, 686, 716, 894
SmlI CTYRAG 2 cut(s) 242, 485
SmoI CTYRAG 2 cut(s) 242, 485
Sse9I AATT 9 cut(s) 428, 435, 615, 900, 1006, 1047, 1098, 1106, 1181
TaiI ACGT 2 cut(s) 37, 322
TaqI TCGA 4 cut(s) 183, 444, 500, 908
TasI AATT 9 cut(s) 428, 435, 615, 900, 1006, 1047, 1098, 1106, 1181
TatI WGTACW 1 cut(s) 384
TauI GCSGC 2 cut(s) 725, 728
TfiI GAWTC 4 cut(s) 89, 269, 859, 872
Tru1I TTAA 4 cut(s) 656, 827, 936, 1202
Tru9I TTAA 4 cut(s) 656, 827, 936, 1202
TscAI CASTG 3 cut(s) 848, 1002, 1203
TseI GCWGC 2 cut(s) 554, 734
TspDTI ATGAA 4 cut(s) 299, 430, 456, 670
TspGWI ACGGA 1 cut(s) 362
TspRI CASTG 3 cut(s) 848, 1002, 1203
VpaK11BI GGWCC 1 cut(s) 460
XagI CCTNNNNNAGG 2 cut(s) 65, 113
XapI RAATTY 2 cut(s) 435, 615
XceI RCATGY 1 cut(s) 595
XcmI CCANNNNNNNNNTGG 1 cut(s) 940
XmnI GAANNNNTTC 1 cut(s) 506
Zsp2I ATGCAT 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.