RchiOBHm_Chr6g0262781

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0010523
Physical Location & Seq
Forward (+)
17561444 .. 17562836
1393 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23566

Sequence Viewer

Length: 1173 bp
ATGGGCGGTGTTCAAGCAAAGCCAGAGAGACGACCCGTGTTGGAGCGAAAGACATCCTTTGCTAAGAATCATAGGATTGTTCCCATCAATTCCGAGCCAAGAATTTTTGAAGAGTTGATGAGATCGTATCAGGCGGATTGCAAAGATGACAAAGAGTTGCAAGACTTCGACGCAGAACTCAGCTCCGGCGCCGAAGAAGTAGTCCGCAGTACCGGTCTTGATCGGTTCAAGCAATGCCTAAAGTACCTGACGCATTCCAACGAGAAGATTGTGAGGGTAATCTTGTCATGCAAAAAACAGATAGTTGAGGCAGAGGGTGTGGGGGCGAGGATGGTGATGACGGCGAATGAGCAGCTCTTGGATCAATTCTTGATCAAGGAGTACTTCAACAATAGCTTGCAGACACTTCAACTCTGCAGATTGGAATTGCAGGACTTTATCAACTCGGCTCGTGAGCTCAACGAGTCTCAGAAGATGTATCACCGTTCTGCGGAAGCAGTTGTTGCCAAGGTGAAATCTCTGCGCGACCAACATAGAGACATGCTTGACAAGTTGAAAGATCGAATTGTGAAGTTTGATAGGAAGCTGGAGCTTACTCGTACTTTAAAGAAGGTCGTAAATGTGGTGTTCATGGGTATGTTGTTTGGTCTGTTTGTTTGCACCATCATGGCGGCGGCTATGGCAGCTCCGCCAGTTGCGTCGGCCTTGACCTCTCATCCATGTATCACCGGAGTTGTTGCTGGTGGTGCCGTTGGTTATGCTTTTTCGGCAGTATTAAAAGAGGGACAACACTGGCTTCTGACTTTTATTCAAGGTTATAAATCAACTCTGGAGGCACATACGGGTGTAATTAAGTCGATGGAAGCCGGAACTAGAGACGCCATTAAAGAGTTGGATGACATCATGTGTCATGTTAATAAGGTCATTGCAAAAGGGAATGATCCAGTGTTGTCCAATTTAAGCTCCGCAAATGATGAGGGAAGAGTGGATGTGGTTGATCAGGAAGTGACGTCTATTCTGGAGAAGCTGGCAGAGTTTGGCAAGGAAATTGAGGAACTGGACGAGAAAAGAGAGCAGTGCCTCCGGGTTATAGAGACTGCAAGGGATAAGGTTCTAGGGGAATATGGCGAATTGAAGAGTAGCCCAGTGTGGAACACGAAGATCAAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

390

Amino Acids

43.9

Weight (kDa)

7.08

Isoelectric Point (pI)

34.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 145 - 373 1.4e-24 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 819
AatII GACGTC 1 cut(s) 1013
AccB1I GGYRCC 2 cut(s) 188, 746
AccII CGCG 1 cut(s) 525
AciI CCGC 8 cut(s) 6, 134, 205, 491, 671, 674, 689, 966
AclWI GGATC 2 cut(s) 369, 935
AcsI RAATTY 1 cut(s) 102
AcyI GRCGYC 3 cut(s) 189, 879, 1010
AfaI GTAC 4 cut(s) 211, 245, 383, 601
AfiI CCNNNNNNNGG 1 cut(s) 490
AgeI ACCGGT 1 cut(s) 212
AgsI TTSAA 8 cut(s) 14, 110, 229, 388, 410, 556, 812, 1135
AluBI AGCT 9 cut(s) 183, 355, 396, 457, 586, 592, 686, 963, 1027
AluI AGCT 9 cut(s) 183, 355, 396, 457, 586, 592, 686, 963, 1027
Alw21I GWGCWC 1 cut(s) 459
Alw26I GTCTC 5 cut(s) 22, 471, 531, 870, 1088
AlwI GGATC 2 cut(s) 369, 935
AoxI GGCC 1 cut(s) 702
ApeKI GCWGC 2 cut(s) 352, 683
ApoI RAATTY 1 cut(s) 102
ArsI GACNNNNNNTTYG 2 cut(s) 186, 218
AsiGI ACCGGT 1 cut(s) 212
AspLEI GCGC 2 cut(s) 191, 525
AsuC2I CCSGG 1 cut(s) 1085
AsuHPI GGTGA 4 cut(s) 346, 473, 523, 718
BanI GGYRCC 2 cut(s) 188, 746
BanII GRGCYC 1 cut(s) 459
BauI CACGAG 1 cut(s) 450
Bbv12I GWGCWC 1 cut(s) 459
BbvI GCAGC 2 cut(s) 364, 695
BccI CCATC 4 cut(s) 92, 325, 671, 853
BceAI ACGGC 2 cut(s) 357, 734
BclI TGATCA 2 cut(s) 372, 997
BcnI CCSGG 1 cut(s) 1085
BcoDI GTCTC 5 cut(s) 22, 471, 531, 870, 1088
BfaI CTAG 2 cut(s) 873, 1115
BfmI CTRYAG 1 cut(s) 415
BfoI RGCGCY 1 cut(s) 192
BisI GCNGC 4 cut(s) 353, 672, 675, 684
BlsI GCNGC 4 cut(s) 354, 673, 676, 685
BmcAI AGTACT 1 cut(s) 383
Bme1390I CCNGG 1 cut(s) 1085
BmiI GGNNCC 2 cut(s) 190, 748
BmrFI CCNGG 1 cut(s) 1085
BmrI ACTGGG 1 cut(s) 1139
BmuI ACTGGG 1 cut(s) 1139
BpmI CTGGAG 3 cut(s) 608, 851, 1040
BpuMI CCSGG 1 cut(s) 1085
BsaHI GRCGYC 3 cut(s) 189, 879, 1010
BsaJI CCNNGG 1 cut(s) 507
BsaWI WCCGGW 2 cut(s) 212, 728
Bsc4I CCNNNNNNNGG 1 cut(s) 490
Bse118I RCCGGY 1 cut(s) 212
Bse1I ACTGG 5 cut(s) 692, 797, 944, 1062, 1145
Bse3DI GCAATG 2 cut(s) 239, 924
BseDI CCNNGG 1 cut(s) 507
BseGI GGATG 5 cut(s) 53, 336, 715, 901, 994
BseLI CCNNNNNNNGG 1 cut(s) 490
BseMI GCAATG 2 cut(s) 239, 924
BseMII CTCAG 2 cut(s) 193, 482
BseNI ACTGG 5 cut(s) 692, 797, 944, 1062, 1145
BseXI GCAGC 2 cut(s) 364, 695
Bsh1236I CGCG 1 cut(s) 525
BshFI GGCC 1 cut(s) 704
BshNI GGYRCC 2 cut(s) 188, 746
BshTI ACCGGT 1 cut(s) 212
BsiHKAI GWGCWC 1 cut(s) 459
BsiSI CCGG 5 cut(s) 186, 213, 729, 867, 1084
BslFI GGGAC 1 cut(s) 798
BslI CCNNNNNNNGG 1 cut(s) 490
BsmAI GTCTC 5 cut(s) 22, 471, 531, 870, 1088
BsmBI CGTCTC 2 cut(s) 22, 870
BsmFI GGGAC 1 cut(s) 798
BsmI GAATGC 1 cut(s) 253
BsnI GGCC 1 cut(s) 704
Bsp1286I GDGCHC 1 cut(s) 459
Bsp143I GATC 8 cut(s) 122, 220, 361, 372, 559, 940, 997, 1161
BspACI CCGC 8 cut(s) 6, 134, 205, 491, 671, 674, 689, 966
BspANI GGCC 1 cut(s) 704
BspCNI CTCAG 2 cut(s) 192, 481
BspFNI CGCG 1 cut(s) 525
BspLI GGNNCC 2 cut(s) 190, 748
BspMAI CTGCAG 1 cut(s) 419
BspPI GGATC 2 cut(s) 369, 935
BspT107I GGYRCC 2 cut(s) 188, 746
BsrDI GCAATG 2 cut(s) 239, 924
BsrFI RCCGGY 1 cut(s) 212
BsrI ACTGG 5 cut(s) 692, 797, 944, 1062, 1145
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 1 cut(s) 507
BssMI GATC 8 cut(s) 122, 220, 361, 372, 559, 940, 997, 1161
BssNI GRCGYC 3 cut(s) 189, 879, 1010
BssSI CACGAG 1 cut(s) 450
BssT1I CCWWGG 1 cut(s) 507
Bst2BI CACGAG 1 cut(s) 450
Bst4CI ACNGT 1 cut(s) 485
Bst6I CTCTTC 3 cut(s) 105, 976, 1130
BstACI GRCGYC 3 cut(s) 189, 879, 1010
BstAPI GCANNNNNTGC 1 cut(s) 503
BstC8I GCNNGC 2 cut(s) 398, 1029
BstDEI CTNAG 3 cut(s) 63, 179, 468
BstF5I GGATG 5 cut(s) 53, 336, 715, 901, 994
BstFNI CGCG 1 cut(s) 525
BstH2I RGCGCY 1 cut(s) 192
BstHHI GCGC 2 cut(s) 191, 525
BstKTI GATC 8 cut(s) 125, 223, 364, 375, 562, 943, 1000, 1164
BstMAI GTCTC 5 cut(s) 22, 471, 531, 870, 1088
BstMBI GATC 8 cut(s) 122, 220, 361, 372, 559, 940, 997, 1161
BstMWI GCNNNNNNNGC 5 cut(s) 503, 680, 683, 746, 767
BstNSI RCATGY 1 cut(s) 544
BstSCI CCNGG 1 cut(s) 1083
BstSFI CTRYAG 1 cut(s) 415
BstUI CGCG 1 cut(s) 525
BstV1I GCAGC 2 cut(s) 364, 695
BsuRI GGCC 1 cut(s) 704
BtsCI GGATG 5 cut(s) 53, 336, 715, 901, 994
BtsI GCAGTG 1 cut(s) 1082
BtsIMutI CAGTG 4 cut(s) 790, 951, 1082, 1152
Cac8I GCNNGC 2 cut(s) 398, 1029
CfoI GCGC 2 cut(s) 191, 525
Cfr10I RCCGGY 1 cut(s) 212
CseI GACGC 4 cut(s) 179, 259, 687, 887
Csp6I GTAC 4 cut(s) 210, 244, 382, 600
CspAI ACCGGT 1 cut(s) 212
CviAII CATG 7 cut(s) 288, 541, 631, 667, 720, 904, 911
CviQI GTAC 4 cut(s) 210, 244, 382, 600
DdeI CTNAG 3 cut(s) 63, 179, 468
DinI GGCGCC 1 cut(s) 190
DpnI GATC 8 cut(s) 124, 222, 363, 374, 561, 942, 999, 1163
DpnII GATC 8 cut(s) 122, 220, 361, 372, 559, 940, 997, 1161
DraI TTTAAA 1 cut(s) 606
Eam1104I CTCTTC 3 cut(s) 105, 976, 1130
EarI CTCTTC 3 cut(s) 105, 976, 1130
EciI GGCGGA 2 cut(s) 149, 678
Ecl136II GAGCTC 1 cut(s) 457
Eco130I CCWWGG 1 cut(s) 507
Eco24I GRGCYC 1 cut(s) 459
Eco53kI GAGCTC 1 cut(s) 457
EcoICRI GAGCTC 1 cut(s) 457
EcoT14I CCWWGG 1 cut(s) 507
EcoT38I GRGCYC 1 cut(s) 459
EgeI GGCGCC 1 cut(s) 190
EheI GGCGCC 1 cut(s) 190
ErhI CCWWGG 1 cut(s) 507
Esp3I CGTCTC 2 cut(s) 22, 870
FaeI CATG 7 cut(s) 291, 544, 634, 670, 723, 907, 914
FalI AAGNNNNNCTT 4 cut(s) 41, 73, 368, 400
FaqI GGGAC 1 cut(s) 798
FatI CATG 7 cut(s) 287, 540, 630, 666, 719, 903, 910
FbaI TGATCA 2 cut(s) 372, 997
Fnu4HI GCNGC 4 cut(s) 353, 672, 675, 684
FokI GGATG 5 cut(s) 40, 343, 702, 908, 1001
FriOI GRGCYC 1 cut(s) 459
Fsp4HI GCNGC 4 cut(s) 353, 672, 675, 684
FspBI CTAG 2 cut(s) 873, 1115
GlaI GCGC 2 cut(s) 190, 524
GluI GCNGC 4 cut(s) 353, 672, 675, 684
GsuI CTGGAG 3 cut(s) 608, 851, 1040
HaeII RGCGCY 1 cut(s) 192
HaeIII GGCC 1 cut(s) 704
HapII CCGG 5 cut(s) 186, 213, 729, 867, 1084
HgaI GACGC 4 cut(s) 179, 259, 687, 887
HhaI GCGC 2 cut(s) 191, 525
Hin1I GRCGYC 3 cut(s) 189, 879, 1010
Hin1II CATG 7 cut(s) 291, 544, 634, 670, 723, 907, 914
Hin6I GCGC 2 cut(s) 189, 523
HinP1I GCGC 2 cut(s) 189, 523
HinfI GANTC 2 cut(s) 67, 464
HpaII CCGG 5 cut(s) 186, 213, 729, 867, 1084
HphI GGTGA 4 cut(s) 346, 473, 523, 718
Hpy188I TCNGA 3 cut(s) 94, 471, 801
Hpy188III TCNNGA 7 cut(s) 218, 370, 452, 830, 1001, 1019, 1165
Hpy99I CGWCG 2 cut(s) 173, 703
HpyAV CCTTC 1 cut(s) 604
HpyCH4III ACNGT 1 cut(s) 485
HpyCH4IV ACGT 1 cut(s) 1010
HpyCH4V TGCA 9 cut(s) 141, 160, 291, 400, 417, 430, 660, 929, 1100
HpyF10VI GCNNNNNNNGC 5 cut(s) 503, 680, 683, 746, 767
HpyF3I CTNAG 3 cut(s) 63, 179, 468
HpySE526I ACGT 1 cut(s) 1010
Hsp92I GRCGYC 3 cut(s) 189, 879, 1010
Hsp92II CATG 7 cut(s) 291, 544, 634, 670, 723, 907, 914
HspAI GCGC 2 cut(s) 189, 523
KasI GGCGCC 1 cut(s) 188
Ksp22I TGATCA 2 cut(s) 372, 997
Kzo9I GATC 8 cut(s) 122, 220, 361, 372, 559, 940, 997, 1161
LmnI GCTCC 5 cut(s) 43, 188, 589, 691, 968
Lsp1109I GCAGC 2 cut(s) 364, 695
MaeI CTAG 2 cut(s) 873, 1115
MaeII ACGT 1 cut(s) 1010
MaeIII GTNAC 1 cut(s) 1006
MalI GATC 8 cut(s) 124, 222, 363, 374, 561, 942, 999, 1163
MboI GATC 8 cut(s) 122, 220, 361, 372, 559, 940, 997, 1161
MboII GAAGA 7 cut(s) 122, 206, 277, 484, 993, 1147, 1171
MhlI GDGCHC 1 cut(s) 459
MluCI AATT 9 cut(s) 88, 102, 365, 425, 564, 849, 955, 1047, 1130
Mly113I GGCGCC 1 cut(s) 189
MlyI GAGTC 1 cut(s) 473
MmeI TCCRAC 3 cut(s) 21, 282, 873
MseI TTAA 6 cut(s) 605, 776, 852, 885, 915, 959
MslI CAYNNNNRTG 3 cut(s) 635, 665, 843
MspI CCGG 5 cut(s) 186, 213, 729, 867, 1084
MspR9I CCNGG 1 cut(s) 1085
Mva1269I GAATGC 1 cut(s) 253
MvnI CGCG 1 cut(s) 525
MwoI GCNNNNNNNGC 5 cut(s) 503, 680, 683, 746, 767
NarI GGCGCC 1 cut(s) 189
NciI CCSGG 1 cut(s) 1085
NdeII GATC 8 cut(s) 122, 220, 361, 372, 559, 940, 997, 1161
NlaIII CATG 7 cut(s) 291, 544, 634, 670, 723, 907, 914
NlaIV GGNNCC 2 cut(s) 190, 748
NmeAIII GCCGAG 1 cut(s) 425
NmuCI GTSAC 1 cut(s) 1006
NspI RCATGY 1 cut(s) 544
PctI GAATGC 1 cut(s) 253
PfeI GAWTC 1 cut(s) 67
PinAI ACCGGT 1 cut(s) 212
PkrI GCNGC 4 cut(s) 354, 673, 676, 685
PleI GAGTC 1 cut(s) 472
PluTI GGCGCC 1 cut(s) 192
PpsI GAGTC 1 cut(s) 472
PsiI TTATAA 1 cut(s) 819
Psp124BI GAGCTC 1 cut(s) 459
PspN4I GGNNCC 2 cut(s) 190, 748
PstI CTGCAG 1 cut(s) 419
RsaI GTAC 4 cut(s) 211, 245, 383, 601
RsaNI GTAC 4 cut(s) 210, 244, 382, 600
RseI CAYNNNNRTG 3 cut(s) 635, 665, 843
SacI GAGCTC 1 cut(s) 459
SaqAI TTAA 6 cut(s) 605, 776, 852, 885, 915, 959
SatI GCNGC 4 cut(s) 353, 672, 675, 684
Sau3AI GATC 8 cut(s) 122, 220, 361, 372, 559, 940, 997, 1161
ScaI AGTACT 1 cut(s) 383
SchI GAGTC 1 cut(s) 473
ScrFI CCNGG 1 cut(s) 1085
SduI GDGCHC 1 cut(s) 459
SfcI CTRYAG 1 cut(s) 415
SfoI GGCGCC 1 cut(s) 190
SmiMI CAYNNNNRTG 3 cut(s) 635, 665, 843
Sse9I AATT 9 cut(s) 88, 102, 365, 425, 564, 849, 955, 1047, 1130
SsiI CCGC 8 cut(s) 6, 134, 205, 491, 671, 674, 689, 966
SspDI GGCGCC 1 cut(s) 188
SspMI CTAG 2 cut(s) 873, 1115
SstI GAGCTC 1 cut(s) 459
StyD4I CCNGG 1 cut(s) 1083
StyI CCWWGG 1 cut(s) 507
TaaI ACNGT 1 cut(s) 485
TaiI ACGT 1 cut(s) 1013
TaqI TCGA 3 cut(s) 168, 562, 857
TasI AATT 9 cut(s) 88, 102, 365, 425, 564, 849, 955, 1047, 1130
TatI WGTACW 1 cut(s) 381
TauI GCSGC 2 cut(s) 674, 677
TfiI GAWTC 1 cut(s) 67
Tru1I TTAA 6 cut(s) 605, 776, 852, 885, 915, 959
Tru9I TTAA 6 cut(s) 605, 776, 852, 885, 915, 959
TscAI CASTG 4 cut(s) 797, 951, 1082, 1152
TseFI GTSAC 1 cut(s) 1006
TseI GCWGC 2 cut(s) 352, 683
Tsp45I GTSAC 1 cut(s) 1006
TspDTI ATGAA 1 cut(s) 619
TspRI CASTG 4 cut(s) 797, 951, 1082, 1152
XapI RAATTY 1 cut(s) 102
XceI RCATGY 1 cut(s) 544
XcmI CCANNNNNNNNNTGG 1 cut(s) 889
XspI CTAG 2 cut(s) 873, 1115
ZraI GACGTC 1 cut(s) 1011
ZrmI AGTACT 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.